Zain Asif Zain - Independent Researcher (original) (raw)

Papers by Zain Asif Zain

Research paper thumbnail of Altered paracellular cation permeability due to a rare CLDN10B variant causes anhidrosis and kidney damage

PLoS genetics, Jan 7, 2017

Claudins constitute the major component of tight junctions and regulate paracellular permeability... more Claudins constitute the major component of tight junctions and regulate paracellular permeability of epithelia. Claudin-10 occurs in two major isoforms that form paracellular channels with ion selectivity. We report on two families segregating an autosomal recessive disorder characterized by generalized anhidrosis, severe heat intolerance and mild kidney failure. All affected individuals carry a rare homozygous missense mutation c.144C>G, p.(N48K) specific for the claudin-10b isoform. Immunostaining of sweat glands from patients suggested that the disease is associated with reduced levels of claudin-10b in the plasma membranes and in canaliculi of the secretory portion. Expression of claudin-10b N48K in a 3D cell model of sweat secretion indicated perturbed paracellular Na+ transport. Analysis of paracellular permeability revealed that claudin-10b N48K maintained cation over anion selectivity but with a reduced general ion conductance. Furthermore, freeze fracture electron micros...

Research paper thumbnail of Kesan luahan kumbahan domestik ke atas sungai dan perairan pantai di Malaysia

Kesan luahan kumbahan domestik ke atas sungai dan perairan pantai di Malaysia

Kajian ke atas luahan kumbahan domestik ke atas sistem sungai dan perairan pantai telah dilakukan... more Kajian ke atas luahan kumbahan domestik ke atas sistem sungai dan perairan pantai telah dilakukan di Kajang (Sg Jeluh dan Langat) dan di Pantai Negeri Sembilan (Port Dickson dan Pantai Tanjung Biru). Parameter kajian tertumpu kepada ciri-ciri utama kumbahan domestik seperti kandungan organik (permintaan oksigen biokimia, BOD dan paras nutrien (fosfat terlarut (PO4) dan nitrat (NO)). Perlepasan kumbahan domestik daripada kolam pengoksidaan di sekitar bandar Kajang telah menyebabkan paras BOD dan PO4 yang tinggi iaitu 1.9-5.1 dan 0.15-2.03 mg/L masing-masing. Peningkatan BOD berkorelasi (r=0.77) dengan penurunan paras oksigen terlarut (DO) dalam air sungai. Pada keseluruhannya kesan kumbahan domestik ke atas Sg. Jeluh dan Langat adalah lebih ketara berbanding dengan yang dialami oleh air marin di persisiran pantai.

Research paper thumbnail of Mechanisms of Mudcake Removal During Flowback

SPE Drilling & Completion, 2001

Summary External filter cakes are used to minimize fluid loss and solids invasion to a formation ... more Summary External filter cakes are used to minimize fluid loss and solids invasion to a formation from drilling and completion fluids. Subsequently, the cake must be removed to increase the flow area and minimize skins, especially for openhole and gravel-packed completions in horizontal and deviated wells. Experimental data is presented to show that the pressure required to initiate flow into the wellbore after building up a filter cake is affected by rock permeability; mud properties, such as particle size and cake yield strength; flowback velocity; and overbalance pressure. Mudcake liftoff tests, mud particle size, and rock pore-throat size distribution measurements were performed to understand the factors that contribute to cake removal. The mechanisms that control filter-cake removal are discussed. In this study, it is clearly shown that the flow-initiation pressure during flowback is controlled by solids and filtrate invasion (i.e., internal formation damage rather than by the e...

Research paper thumbnail of Guidelines for Management of COPD

Guidelines for Management of COPD

Ministry of Health Malaysia, Academy of Medicine of …, 1998

Research paper thumbnail of A Proposal for the Measurement of Quality Position In UK Manufacturing Industries

A Proposal for the Measurement of Quality Position In UK Manufacturing Industries

Research paper thumbnail of Unilateral L4-dorsal root ganglion stimulation evokes pain relief in chronic neuropathic postsurgical knee pain and changes of inflammatory markers: part II whole transcriptome profiling

Journal of Translational Medicine, 2019

Background In our recent clinical trial, increased peripheral concentrations of pro-inflammatory ... more Background In our recent clinical trial, increased peripheral concentrations of pro-inflammatory molecular mediators were determined in complex regional pain syndrome (CRPS) patients. After 3 months adjunctive unilateral, selective L4 dorsal root ganglion stimulation (L4-DRGSTIM), significantly decreased serum IL-10 and increased saliva oxytocin levels were assessed along with an improved pain and functional state. The current study extended molecular profiling towards gene expression analysis of genes known to be involved in the gonadotropin releasing hormone receptor and neuroinflammatory (cytokines/chemokines) signaling pathways. Methods Blood samples were collected from 12 CRPS patients for whole-transcriptome profiling in order to assay 18,845 inflammation-associated genes from frozen blood at baseline and after 3 months L4-DRGSTIM using PANTHER™ pathway enrichment analysis tool. Results Pathway enrichment analyses tools (GOrilla™ and PANTHER™) showed predominant involvement of...

Research paper thumbnail of Altered paracellular cation permeability due to a rare CLDN10B variant causes anhidrosis and kidney damage

PLoS genetics, Jan 7, 2017

Claudins constitute the major component of tight junctions and regulate paracellular permeability... more Claudins constitute the major component of tight junctions and regulate paracellular permeability of epithelia. Claudin-10 occurs in two major isoforms that form paracellular channels with ion selectivity. We report on two families segregating an autosomal recessive disorder characterized by generalized anhidrosis, severe heat intolerance and mild kidney failure. All affected individuals carry a rare homozygous missense mutation c.144C>G, p.(N48K) specific for the claudin-10b isoform. Immunostaining of sweat glands from patients suggested that the disease is associated with reduced levels of claudin-10b in the plasma membranes and in canaliculi of the secretory portion. Expression of claudin-10b N48K in a 3D cell model of sweat secretion indicated perturbed paracellular Na+ transport. Analysis of paracellular permeability revealed that claudin-10b N48K maintained cation over anion selectivity but with a reduced general ion conductance. Furthermore, freeze fracture electron micros...

Research paper thumbnail of Kesan luahan kumbahan domestik ke atas sungai dan perairan pantai di Malaysia

Kesan luahan kumbahan domestik ke atas sungai dan perairan pantai di Malaysia

Kajian ke atas luahan kumbahan domestik ke atas sistem sungai dan perairan pantai telah dilakukan... more Kajian ke atas luahan kumbahan domestik ke atas sistem sungai dan perairan pantai telah dilakukan di Kajang (Sg Jeluh dan Langat) dan di Pantai Negeri Sembilan (Port Dickson dan Pantai Tanjung Biru). Parameter kajian tertumpu kepada ciri-ciri utama kumbahan domestik seperti kandungan organik (permintaan oksigen biokimia, BOD dan paras nutrien (fosfat terlarut (PO4) dan nitrat (NO)). Perlepasan kumbahan domestik daripada kolam pengoksidaan di sekitar bandar Kajang telah menyebabkan paras BOD dan PO4 yang tinggi iaitu 1.9-5.1 dan 0.15-2.03 mg/L masing-masing. Peningkatan BOD berkorelasi (r=0.77) dengan penurunan paras oksigen terlarut (DO) dalam air sungai. Pada keseluruhannya kesan kumbahan domestik ke atas Sg. Jeluh dan Langat adalah lebih ketara berbanding dengan yang dialami oleh air marin di persisiran pantai.

Research paper thumbnail of Mechanisms of Mudcake Removal During Flowback

SPE Drilling & Completion, 2001

Summary External filter cakes are used to minimize fluid loss and solids invasion to a formation ... more Summary External filter cakes are used to minimize fluid loss and solids invasion to a formation from drilling and completion fluids. Subsequently, the cake must be removed to increase the flow area and minimize skins, especially for openhole and gravel-packed completions in horizontal and deviated wells. Experimental data is presented to show that the pressure required to initiate flow into the wellbore after building up a filter cake is affected by rock permeability; mud properties, such as particle size and cake yield strength; flowback velocity; and overbalance pressure. Mudcake liftoff tests, mud particle size, and rock pore-throat size distribution measurements were performed to understand the factors that contribute to cake removal. The mechanisms that control filter-cake removal are discussed. In this study, it is clearly shown that the flow-initiation pressure during flowback is controlled by solids and filtrate invasion (i.e., internal formation damage rather than by the e...

Research paper thumbnail of Guidelines for Management of COPD

Guidelines for Management of COPD

Ministry of Health Malaysia, Academy of Medicine of …, 1998

Research paper thumbnail of A Proposal for the Measurement of Quality Position In UK Manufacturing Industries

A Proposal for the Measurement of Quality Position In UK Manufacturing Industries

Research paper thumbnail of Unilateral L4-dorsal root ganglion stimulation evokes pain relief in chronic neuropathic postsurgical knee pain and changes of inflammatory markers: part II whole transcriptome profiling

Journal of Translational Medicine, 2019

Background In our recent clinical trial, increased peripheral concentrations of pro-inflammatory ... more Background In our recent clinical trial, increased peripheral concentrations of pro-inflammatory molecular mediators were determined in complex regional pain syndrome (CRPS) patients. After 3 months adjunctive unilateral, selective L4 dorsal root ganglion stimulation (L4-DRGSTIM), significantly decreased serum IL-10 and increased saliva oxytocin levels were assessed along with an improved pain and functional state. The current study extended molecular profiling towards gene expression analysis of genes known to be involved in the gonadotropin releasing hormone receptor and neuroinflammatory (cytokines/chemokines) signaling pathways. Methods Blood samples were collected from 12 CRPS patients for whole-transcriptome profiling in order to assay 18,845 inflammation-associated genes from frozen blood at baseline and after 3 months L4-DRGSTIM using PANTHER™ pathway enrichment analysis tool. Results Pathway enrichment analyses tools (GOrilla™ and PANTHER™) showed predominant involvement of...