Zeynal Akparov - Academia.edu (original) (raw)

Papers by Zeynal Akparov

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2018

The genetic relationships among 48 melon (Cucumis melo L.) genotypes collected from various parts... more The genetic relationships among 48 melon (Cucumis melo L.) genotypes collected from various parts of Azerbaijan were determined by comparing their phenotypic and molecular traits. Eleven agromorphological traits and 10 polymorphic inter-simple sequence repeat (ISSR) primers were used to define the genetic diversity. Principal component analysis grouped the agromorphological traits into the first four axes, describing 78% of the total variations. The highest genetic variation coefficient was found for yield per hectare (20.32%) and for fruit length (17.35%). Calculated heritability for yield was 0.96. The analysis of morphological traits grouped the accessions into four clusters. The 10 ISSR primers yielded 35 polymorphic alleles, representing 85.4% of all the amplified loci. The average genetic diversity index determined was 0.70. The highest and the lowest similarity indexes were equal to 0.97 and 0.36, respectively. The 48 accessions were grouped into 10 clusters based on ISSR markers. Correlation between distance matrices based on agromorphological traits and ISSR markers was not statistically significant (r = 0.012).

These studies aimed creation of universal models suitable for plants belonging to various taxonom... more These studies aimed creation of universal models suitable for plants belonging to various taxonomic groups, and enabling to give functional diagnostics and genetic prognosis of viability potentials and longevity of their seed samples. In the example of plants belonging to diverse taxonomic groups, new approach is proposed for functional diagnostics of viability potential of seeds and their longevity in individual samples and for making genetic prognosis. Based on this, universal practical models with high decisive capacity have been elaborated and quality factors have been worked out.

BackgroundMembers of the eukaryotic translation initiation complex are co-opted in viral infectio... more BackgroundMembers of the eukaryotic translation initiation complex are co-opted in viral infection, leading to susceptibility in many crop species, including stone fruit trees (Prunus spp). Therefore, modification of one of those eukaryotic translation initiation factors or changes in their gene expression may result in resistance.ObjectiveWe searched the crop and wildPrunusgermplasm from the Armeniaca and Amygdalus taxonomic sections for allelic variants in theeIF4EandeIFiso4Egenes, to identify alleles potentially linked to resistance to thePlum Pox Virus(PPV).Methodology and resultsOver one thousand stone fruit accessions (1,397) were screened for variation in eIF4E and eIFiso4E transcript sequences which are in single copy within the diploidPrunusgenome. We identified new alleles for both genes that are not evident in haplotypes associated with PPV susceptible individuals. Overall, analyses showed thateIFiso4Eis genetically more constrained since it displayed less polymorphism th...

Plants

The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, an... more The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalf...

Journal of Life Sciences and Biomedicine of ANAS, 2021

This paper gives an overview of the collection history for the species from the Triticeae Dum. tr... more This paper gives an overview of the collection history for the species from the Triticeae Dum. tribe in the Karabakh region of Azerbaijan up to date of its occupation. By covering most of the Lesser Caucasus with the semi-desert and dry steppe climate, the Karabakh region of Azerbaijan has favorable conditions for grass diversity. There are 14 genera from the tribe Triticeae in Azerbaijan, of which historically the 10 genera also were found in the Karabakh region. The data in this article have been presented as a summary of information recorded in different literature sources, that would be useful for the planned monitoring of status, distribution, and preserving issues for the Triticeae species in the liberated areas.

This file contains two sheets: the first one describes the samples and their locations while the ... more This file contains two sheets: the first one describes the samples and their locations while the second sheet presents the genotypes at 20 SSR and SSLP loci as they were used for diversity and differentiation analysis in the MEC-16-0220 manuscript.N/A: missing data

Studying domesticated species and their wild relatives allows understanding of the mechanisms of ... more Studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. Apricot is an important fruit in the Northern hemisphere, where it is threatened by the Plum pox virus (PPV), causing the sharka disease. The histories of apricot domestication and of its resistance to sharka are however still poorly understood. We used 18 microsatellite markers to genotype a collection of 230 wild trees from Central Asia and 142 cultivated apricots as representatives of the worldwide cultivated apricot germplasm; we also performed experimental PPV inoculation tests. The genetic markers revealed highest levels of diversity in Central Asian and Chinese wild and cultivated apricots, confirming an origin in this region. In cultivated apricots, Chinese accessions were differentiated from more Western accessions, while cultivated apricots were differentiated from wild apricots. An approximate Bayesian approach indicated that apricots likely underwent two independent domestication events, with bottlenecks, from the same wild population. Central Asian native apricots exhibited genetic subdivision and high frequency of resistance to sharka. Altogether, our results contribute to the understanding of the domestication history of cultivated apricot and point to valuable genetic diversity in the extant genetic resources of wild apricots

Journal of Life Sciences and Biomedicine of ANAS, 2021

Agrobiological features of new perspective durum wheat varieties "Maya", "Alliance" and bread whe... more Agrobiological features of new perspective durum wheat varieties "Maya", "Alliance" and bread wheat varieties "Leyla", "Start", "Janub", "Khamsa", "Almaz", "Oguz", "Vilash" created as a result of many years of research (2005-2020) of the Genetic Resources Institute (GRI) of ANAS and the Scientific Research Institute of Crop Husbandry (RICH) in the Karabakh region were compared. The varieties were characterized by high adaptability and potential productivity. Among them "Start" (patent № 00246), "Janub" (patent № 00274), "Leyla" (patent № 00305) and "Almaz" (patent № 00312) varieties of bread wheat, "Maya" (patent № 00250) and "Alliance" (patent № 00316) varieties of durum wheat were patented by the Agrarian Services Agency and regionalized. The studied varieties were analyzed for height, productivity and structural elements, resistance to rust diseases and adaptability in the competitive variety testing nursery. In the year of epiphytosis (2020), durum wheat varieties and "Almaz" and "Khamsa" bread wheat varieties were resistant to yellow rust. Significant differences were also observed in terms of productivity and structural elements. In addition, the new durum and bread wheat varieties were characterized by morphobiological and agronomic features and characteristics. Optimal cultivation technology of regionalized varieties, predecessors, fertilizer norms and sowing period were also given. It is recommended to cultivate new varieties in the irrigated plains and moisture-supplied foothills of the Karabakh region.

Biodiversity, Conservation and Sustainability in Asia, 2021

The role of agrodiversity is of great importance for Azerbaijan because it ensures food security ... more The role of agrodiversity is of great importance for Azerbaijan because it ensures food security in the country. Cereals (mainly wheat, barley), grain legumes (mainly chickpea, lentil), vegetable-melon crops (mainly tomato, watermelon, melon), leguminous-forage crops (alfalfa), fruits (apple, pear, pomegranate, fig, walnut, hazelnut), grape, and industrial crops (cotton) have been cultivated historically and developed by means of selection by the local farmers to meet the demands of its population. These are considered as the major crops of the agricultural system in the country even today. These crops and their wild relatives along with other wild plants are used by people for food, as medicinal plants, and for other purposes. About 1230 species of the flora of Azerbaijan serve as wild relatives of crops (with the exception of ornamentals and medicinal plants). Azerbaijan is considered the center of origin of some cereals such as barley, nine species of Aegilops, five species of rye, as well as different fodders, fruits, vegetables, melons, and other crops. This chapter reviews the general features of agrodiversity of the country.

The aim of this study was the assessment of the genetic diversity of 60 accessions, comprising 30... more The aim of this study was the assessment of the genetic diversity of 60 accessions, comprising 30 local table grape varieties ( Vitis vinifera ssp. sativa ) and 30 wild types ( Vitis vinifera ssp. sylvestris) originating from regions near the Caspian Sea of Azerbaijan Republic by the multivariate statistics analysis of morphological traits. All plant materials are belonged to grape collections of the Azerbaijan Genetic Resources Institute of ANAS and Azerbaijan Scientific Research Institute of Viticulture and Wine-making. The experiment was arranged in a randomized completely block design (RCBD) with three two-vine replications. For each accession, 23 morphological traits (length of annual bud, length and width leave, length of stalk leave, length of central vessel, number of bunches per bush, length and width bunch, length of stalk bunch, number of berry per bunch, length and width berry, weight of bunch, number of seeds, length and width seed, 100 weight of berries, 100 weight of ...

Genotyping-by-sequencing (GBS) is a genetic screening method for discovering and genotyping novel... more Genotyping-by-sequencing (GBS) is a genetic screening method for discovering and genotyping novel single nucleotide polymorphisms (SNPs) in crop genomes and populations. In the current research a phenotypic and genotypic assessment of 76 durum wheat (Triticum durum Desf.) accessions of Azerbaijan origin was made using six phenotypic traits and GBS technology. After screening for leaf and stem rust resistance at the seedling stage, 16 genotypes displayed resistance to leaf rust and 14 to stem rust. Some relationships were found between resistance to leaf rust and phenotypic traits of botanical varieties. The highest Pearson’s correlation (r=0.53; p < 0.001) was noted between awn color and pubescence. The durum wheat genotypes fell into four main groups in the clustered heat map; grouping according to botanical variety. A total of 748 SNP markers were obtained for the collection. The average polymorphic information content and genetic diversity index for the entire collection were ...

Legume Research, 2018

Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using I... more Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.

The wild relatives of major fruits and nuts like grapevine, apple, pear, quince, medlar, pomegran... more The wild relatives of major fruits and nuts like grapevine, apple, pear, quince, medlar, pomegranate, fig, cherry, apricot, almond, hazelnut, walnut, chestnut, pistachio and others are presented along with many genera of the flora of Azerbaijan. The activity of the Genetic Resources Institute (GRI) of the Azerbaijan National Academy of Science (ANAS) is collections, reproduction, studies, documentation and conservation of the gene pool of local resources of landraces of fruit plants, including their crop wild relatives (CWRs). The CWRs in Azerbaijan are conserved in protected areas and botanical gardens, as well as ex situ and on-farm conservation in field collections of the National Genebank. Collecting of plant genetic resources (PGR) of fruit-berry crops and grape has been enhanced in the last years. At present 2490 accessions of fruit plants are maintained in field collections of GRI. Nearly 300 grape landraces and about 50 samples of wild grapes have been collected by scientist...

To understand genotypic responses of wheat genotypes to drought stress, this project studied 64 d... more To understand genotypic responses of wheat genotypes to drought stress, this project studied 64 different wheat genotypes under both irrigated and drought stress conditions, using simple lattice experimental design with two replication in research area of agricultural research station of Ardabil and research area and laboratory of genetic resources institute of Azerbaijan during two seasons 2006 to 2007. Drought reduced wheat grain yield by 61.9% and stress intensity (SI) for wheat grain yield was 0.62. The genotypes studied were classified into three groups; tolerant, moderate tolerance and susceptible by genotypic responses of wheat genotypes under both irrigated and drought stress conditions and on the basis of values of SSI and STI indices. Results of germination capability of wheat genotypes evaluated under 16 atmosphere drought stress of PEG and control (water) treatments showed the same results as field experiments on the basis of the classifications of wheat genotypes for th...

Molecular Biology Reports, 2021

Olive (Olea europaea L.) is one of the most economically important crop from east to the west aro... more Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalık 1'and 'Ayvalık 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalık" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.

Faktori eksperimental'noi evolucii organizmiv, 2018

Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected... more Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected from different regions of Azerbaijan. Methods. Molecular-genetic. Results. The mean polymorphism level for the studied genotypes of bread wheat, using 8 ISSR markers, was 72 %. An index of genetic diversity calculated for the studied collection of T. aestivum specie was equal to 0.91. Among the bread wheat accessions, the highest genetic diversity was established for botanical variety var. erythrospermum (GDI=0.90). Conclusions. Primers UBC 112, UBC 841, UBC 857 and UBC 873 were identified to be more effective in identification of bread wheat accessions. As a result of the GGE biplot analysis, a trait collection was created by selecting 20 bread wheat samples with broad genetic variation. А core collection of bread wheat was created, which coversthe most distant genotypes and represents the genetic diversity of all 110 accessions studied. Keywords: Triticum aestivum Desf., genetic polym...

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2018

The genetic relationships among 48 melon (Cucumis melo L.) genotypes collected from various parts... more The genetic relationships among 48 melon (Cucumis melo L.) genotypes collected from various parts of Azerbaijan were determined by comparing their phenotypic and molecular traits. Eleven agromorphological traits and 10 polymorphic inter-simple sequence repeat (ISSR) primers were used to define the genetic diversity. Principal component analysis grouped the agromorphological traits into the first four axes, describing 78% of the total variations. The highest genetic variation coefficient was found for yield per hectare (20.32%) and for fruit length (17.35%). Calculated heritability for yield was 0.96. The analysis of morphological traits grouped the accessions into four clusters. The 10 ISSR primers yielded 35 polymorphic alleles, representing 85.4% of all the amplified loci. The average genetic diversity index determined was 0.70. The highest and the lowest similarity indexes were equal to 0.97 and 0.36, respectively. The 48 accessions were grouped into 10 clusters based on ISSR markers. Correlation between distance matrices based on agromorphological traits and ISSR markers was not statistically significant (r = 0.012).

These studies aimed creation of universal models suitable for plants belonging to various taxonom... more These studies aimed creation of universal models suitable for plants belonging to various taxonomic groups, and enabling to give functional diagnostics and genetic prognosis of viability potentials and longevity of their seed samples. In the example of plants belonging to diverse taxonomic groups, new approach is proposed for functional diagnostics of viability potential of seeds and their longevity in individual samples and for making genetic prognosis. Based on this, universal practical models with high decisive capacity have been elaborated and quality factors have been worked out.

BackgroundMembers of the eukaryotic translation initiation complex are co-opted in viral infectio... more BackgroundMembers of the eukaryotic translation initiation complex are co-opted in viral infection, leading to susceptibility in many crop species, including stone fruit trees (Prunus spp). Therefore, modification of one of those eukaryotic translation initiation factors or changes in their gene expression may result in resistance.ObjectiveWe searched the crop and wildPrunusgermplasm from the Armeniaca and Amygdalus taxonomic sections for allelic variants in theeIF4EandeIFiso4Egenes, to identify alleles potentially linked to resistance to thePlum Pox Virus(PPV).Methodology and resultsOver one thousand stone fruit accessions (1,397) were screened for variation in eIF4E and eIFiso4E transcript sequences which are in single copy within the diploidPrunusgenome. We identified new alleles for both genes that are not evident in haplotypes associated with PPV susceptible individuals. Overall, analyses showed thateIFiso4Eis genetically more constrained since it displayed less polymorphism th...

Plants

The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, an... more The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalf...

Journal of Life Sciences and Biomedicine of ANAS, 2021

This paper gives an overview of the collection history for the species from the Triticeae Dum. tr... more This paper gives an overview of the collection history for the species from the Triticeae Dum. tribe in the Karabakh region of Azerbaijan up to date of its occupation. By covering most of the Lesser Caucasus with the semi-desert and dry steppe climate, the Karabakh region of Azerbaijan has favorable conditions for grass diversity. There are 14 genera from the tribe Triticeae in Azerbaijan, of which historically the 10 genera also were found in the Karabakh region. The data in this article have been presented as a summary of information recorded in different literature sources, that would be useful for the planned monitoring of status, distribution, and preserving issues for the Triticeae species in the liberated areas.

This file contains two sheets: the first one describes the samples and their locations while the ... more This file contains two sheets: the first one describes the samples and their locations while the second sheet presents the genotypes at 20 SSR and SSLP loci as they were used for diversity and differentiation analysis in the MEC-16-0220 manuscript.N/A: missing data

Studying domesticated species and their wild relatives allows understanding of the mechanisms of ... more Studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. Apricot is an important fruit in the Northern hemisphere, where it is threatened by the Plum pox virus (PPV), causing the sharka disease. The histories of apricot domestication and of its resistance to sharka are however still poorly understood. We used 18 microsatellite markers to genotype a collection of 230 wild trees from Central Asia and 142 cultivated apricots as representatives of the worldwide cultivated apricot germplasm; we also performed experimental PPV inoculation tests. The genetic markers revealed highest levels of diversity in Central Asian and Chinese wild and cultivated apricots, confirming an origin in this region. In cultivated apricots, Chinese accessions were differentiated from more Western accessions, while cultivated apricots were differentiated from wild apricots. An approximate Bayesian approach indicated that apricots likely underwent two independent domestication events, with bottlenecks, from the same wild population. Central Asian native apricots exhibited genetic subdivision and high frequency of resistance to sharka. Altogether, our results contribute to the understanding of the domestication history of cultivated apricot and point to valuable genetic diversity in the extant genetic resources of wild apricots

Journal of Life Sciences and Biomedicine of ANAS, 2021

Agrobiological features of new perspective durum wheat varieties "Maya", "Alliance" and bread whe... more Agrobiological features of new perspective durum wheat varieties "Maya", "Alliance" and bread wheat varieties "Leyla", "Start", "Janub", "Khamsa", "Almaz", "Oguz", "Vilash" created as a result of many years of research (2005-2020) of the Genetic Resources Institute (GRI) of ANAS and the Scientific Research Institute of Crop Husbandry (RICH) in the Karabakh region were compared. The varieties were characterized by high adaptability and potential productivity. Among them "Start" (patent № 00246), "Janub" (patent № 00274), "Leyla" (patent № 00305) and "Almaz" (patent № 00312) varieties of bread wheat, "Maya" (patent № 00250) and "Alliance" (patent № 00316) varieties of durum wheat were patented by the Agrarian Services Agency and regionalized. The studied varieties were analyzed for height, productivity and structural elements, resistance to rust diseases and adaptability in the competitive variety testing nursery. In the year of epiphytosis (2020), durum wheat varieties and "Almaz" and "Khamsa" bread wheat varieties were resistant to yellow rust. Significant differences were also observed in terms of productivity and structural elements. In addition, the new durum and bread wheat varieties were characterized by morphobiological and agronomic features and characteristics. Optimal cultivation technology of regionalized varieties, predecessors, fertilizer norms and sowing period were also given. It is recommended to cultivate new varieties in the irrigated plains and moisture-supplied foothills of the Karabakh region.

Biodiversity, Conservation and Sustainability in Asia, 2021

The role of agrodiversity is of great importance for Azerbaijan because it ensures food security ... more The role of agrodiversity is of great importance for Azerbaijan because it ensures food security in the country. Cereals (mainly wheat, barley), grain legumes (mainly chickpea, lentil), vegetable-melon crops (mainly tomato, watermelon, melon), leguminous-forage crops (alfalfa), fruits (apple, pear, pomegranate, fig, walnut, hazelnut), grape, and industrial crops (cotton) have been cultivated historically and developed by means of selection by the local farmers to meet the demands of its population. These are considered as the major crops of the agricultural system in the country even today. These crops and their wild relatives along with other wild plants are used by people for food, as medicinal plants, and for other purposes. About 1230 species of the flora of Azerbaijan serve as wild relatives of crops (with the exception of ornamentals and medicinal plants). Azerbaijan is considered the center of origin of some cereals such as barley, nine species of Aegilops, five species of rye, as well as different fodders, fruits, vegetables, melons, and other crops. This chapter reviews the general features of agrodiversity of the country.

The aim of this study was the assessment of the genetic diversity of 60 accessions, comprising 30... more The aim of this study was the assessment of the genetic diversity of 60 accessions, comprising 30 local table grape varieties ( Vitis vinifera ssp. sativa ) and 30 wild types ( Vitis vinifera ssp. sylvestris) originating from regions near the Caspian Sea of Azerbaijan Republic by the multivariate statistics analysis of morphological traits. All plant materials are belonged to grape collections of the Azerbaijan Genetic Resources Institute of ANAS and Azerbaijan Scientific Research Institute of Viticulture and Wine-making. The experiment was arranged in a randomized completely block design (RCBD) with three two-vine replications. For each accession, 23 morphological traits (length of annual bud, length and width leave, length of stalk leave, length of central vessel, number of bunches per bush, length and width bunch, length of stalk bunch, number of berry per bunch, length and width berry, weight of bunch, number of seeds, length and width seed, 100 weight of berries, 100 weight of ...

Genotyping-by-sequencing (GBS) is a genetic screening method for discovering and genotyping novel... more Genotyping-by-sequencing (GBS) is a genetic screening method for discovering and genotyping novel single nucleotide polymorphisms (SNPs) in crop genomes and populations. In the current research a phenotypic and genotypic assessment of 76 durum wheat (Triticum durum Desf.) accessions of Azerbaijan origin was made using six phenotypic traits and GBS technology. After screening for leaf and stem rust resistance at the seedling stage, 16 genotypes displayed resistance to leaf rust and 14 to stem rust. Some relationships were found between resistance to leaf rust and phenotypic traits of botanical varieties. The highest Pearson’s correlation (r=0.53; p < 0.001) was noted between awn color and pubescence. The durum wheat genotypes fell into four main groups in the clustered heat map; grouping according to botanical variety. A total of 748 SNP markers were obtained for the collection. The average polymorphic information content and genetic diversity index for the entire collection were ...

Legume Research, 2018

Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using I... more Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.

The wild relatives of major fruits and nuts like grapevine, apple, pear, quince, medlar, pomegran... more The wild relatives of major fruits and nuts like grapevine, apple, pear, quince, medlar, pomegranate, fig, cherry, apricot, almond, hazelnut, walnut, chestnut, pistachio and others are presented along with many genera of the flora of Azerbaijan. The activity of the Genetic Resources Institute (GRI) of the Azerbaijan National Academy of Science (ANAS) is collections, reproduction, studies, documentation and conservation of the gene pool of local resources of landraces of fruit plants, including their crop wild relatives (CWRs). The CWRs in Azerbaijan are conserved in protected areas and botanical gardens, as well as ex situ and on-farm conservation in field collections of the National Genebank. Collecting of plant genetic resources (PGR) of fruit-berry crops and grape has been enhanced in the last years. At present 2490 accessions of fruit plants are maintained in field collections of GRI. Nearly 300 grape landraces and about 50 samples of wild grapes have been collected by scientist...

To understand genotypic responses of wheat genotypes to drought stress, this project studied 64 d... more To understand genotypic responses of wheat genotypes to drought stress, this project studied 64 different wheat genotypes under both irrigated and drought stress conditions, using simple lattice experimental design with two replication in research area of agricultural research station of Ardabil and research area and laboratory of genetic resources institute of Azerbaijan during two seasons 2006 to 2007. Drought reduced wheat grain yield by 61.9% and stress intensity (SI) for wheat grain yield was 0.62. The genotypes studied were classified into three groups; tolerant, moderate tolerance and susceptible by genotypic responses of wheat genotypes under both irrigated and drought stress conditions and on the basis of values of SSI and STI indices. Results of germination capability of wheat genotypes evaluated under 16 atmosphere drought stress of PEG and control (water) treatments showed the same results as field experiments on the basis of the classifications of wheat genotypes for th...

Molecular Biology Reports, 2021

Olive (Olea europaea L.) is one of the most economically important crop from east to the west aro... more Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalık 1'and 'Ayvalık 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalık" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.

Faktori eksperimental'noi evolucii organizmiv, 2018

Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected... more Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected from different regions of Azerbaijan. Methods. Molecular-genetic. Results. The mean polymorphism level for the studied genotypes of bread wheat, using 8 ISSR markers, was 72 %. An index of genetic diversity calculated for the studied collection of T. aestivum specie was equal to 0.91. Among the bread wheat accessions, the highest genetic diversity was established for botanical variety var. erythrospermum (GDI=0.90). Conclusions. Primers UBC 112, UBC 841, UBC 857 and UBC 873 were identified to be more effective in identification of bread wheat accessions. As a result of the GGE biplot analysis, a trait collection was created by selecting 20 bread wheat samples with broad genetic variation. А core collection of bread wheat was created, which coversthe most distant genotypes and represents the genetic diversity of all 110 accessions studied. Keywords: Triticum aestivum Desf., genetic polym...