Zisis Kozlakidis - Academia.edu (original) (raw)

Papers by Zisis Kozlakidis

Cybergeo, 2009

... Matthieu Ghilardi, Éric Fouache, George Syrides, Mike Styllas, Stathis Stiros et Zisis Kozlak... more ... Matthieu Ghilardi, Éric Fouache, George Syrides, Mike Styllas, Stathis Stiros et Zisis Kozlakidis. ... à proximité du trait de côte, à l'arrière d'un cordon littoral sableux où subsistent des lagunes ; c'est la vision que nous propose Leake (1835, figure 3). Félix de Beaujour vante à ce ...

International Journal of Molecular Sciences

Long COVID, also called post-acute sequelae of SARS-CoV-2, is characterized by a multitude of lin... more Long COVID, also called post-acute sequelae of SARS-CoV-2, is characterized by a multitude of lingering symptoms, including impaired cognition, that can last for many months. This symptom, often called “brain fog”, affects the life quality of numerous individuals, increasing medical complications as well as healthcare expenditures. The etiopathogenesis of SARS-CoV-2-induced cognitive deficit is unclear, but the most likely cause is chronic inflammation maintained by a viral remnant thriving in select body reservoirs. These viral sanctuaries are likely comprised of fused, senescent cells, including microglia and astrocytes, that the pathogen can convert into neurotoxic phenotypes. Moreover, as the enteric nervous system contains neurons and glia, the virus likely lingers in the gastrointestinal tract as well, accounting for the intestinal symptoms of long COVID. Fusogens are proteins that can overcome the repulsive forces between cell membranes, allowing the virus to coalesce with ho...

Open Research Europe

The exposome is a complex scientific field that has enjoyed consistent growth over the last two d... more The exposome is a complex scientific field that has enjoyed consistent growth over the last two decades, defined as the composite of every exposure to which an individual is subjected from conception to death. The study of the exposome requires consideration of both the nature of those exposures and their changes over time, and as such necessitates high quality data and software solutions. As the exposome is both a broad and a recent concept, it is challenging to define or to introduce in a structured way. Thus, an approach to assist with clear definitions and a structured framework is needed for the wider scientific and public communication. Results: A set of 14 personas were developed through three focus groups and a series of 14 semi-structured interviews. The focus groups defined the broad themes specific to exposome research, while the sub-themes emerged to saturation via the interviews process. Personas are imaginary individuals that represent segments/groups of real people wi...

Applied Microbiology

Gut microbes are immunologically tolerated in the gastrointestinal tract but trigger aggressive i... more Gut microbes are immunologically tolerated in the gastrointestinal tract but trigger aggressive immune responses upon translocation across the gut barrier. Although oral tolerance, a physiological process that dampens immune responses to food proteins and commensal microbiota, remains poorly defined, significant progress was made during and after the Human Immunodeficiency Virus epidemic in the 1980s and the discovery of regulatory T cells in 1995. Additional insight was gained after the discoveries of innate lymphoid cells in 2008 and the functional elucidation of mucosal mast cells. Prior to the historical discovery of human pathogens, the etiologies of most human diseases were considered unknown. The same was true about many genetic disorders prior to the Human Genome Project. Here, we hypothesize that many of the remaining idiopathic conditions, including autoimmune, fibroproliferative, and neuropsychiatric diseases as well as some cancers, can be considered microbial translocat...

Biopreservation and Biobanking

Innovations in Digital Health, Diagnostics, and Biomarkers

Biobanks are foundational infrastructures that collect and provide standardized, high-quality, an... more Biobanks are foundational infrastructures that collect and provide standardized, high-quality, and research-ready biological material and associated data. The advent of high-throughput technologies has further supported the creation of biobanks globally. However, the same rate of growth has not occurred in the field of infectious diseases, where biobanks are significantly fewer as compared to other scientific fields such as oncology. This narrative review presents the main aspects that need to be taken into consideration for the construction and application of biological sample infrastructure for infectious diseases, with a number of examples taken from the recent COVID-19 pandemic.

Reports

Infection with SARS-CoV-2, the causative agent of the COVID-19 pandemic, originated in China and ... more Infection with SARS-CoV-2, the causative agent of the COVID-19 pandemic, originated in China and quickly spread across the globe. Despite tremendous economic and healthcare devastation, research on this virus has contributed to a better understanding of numerous molecular pathways, including those involving γ-aminobutyric acid (GABA), that will positively impact medical science, including neuropsychiatry, in the post-pandemic era. SARS-CoV-2 primarily enters the host cells through the renin–angiotensin system’s component named angiotensin-converting enzyme-2 (ACE-2). Among its many functions, this protein upregulates GABA, protecting not only the central nervous system but also the endothelia, the pancreas, and the gut microbiota. SARS-CoV-2 binding to ACE-2 usurps the neuronal and non-neuronal GABAergic systems, contributing to the high comorbidity of neuropsychiatric illness with gut dysbiosis and endothelial and metabolic dysfunctions. In this perspective article, we take a close...

Archives of Iranian Medicine, 2021

Background: We aim to present the development and the initial results of the Golestan Cancer Biob... more Background: We aim to present the development and the initial results of the Golestan Cancer Biobank (GoCB), in a low resource setting in northern Iran. Methods: The GoCB protocol and its standard operation procedures (SOP) were developed according to internationally accepted standards and protocols with some modifications considering the limited resources in our setting. The main biological samples collected by the GoCB include blood sample, urine sample, fresh endoscopy tissue sample, fresh surgical tissue sample and formalin fixed paraffin embedded (FFPE) tissue sample. The GoCB collects patients' demographic data, tumor characteristics as well as data on risk factors. We developed a specific GoCB software for management of patient data and biological sample information. The GoCB dataset is annually linked with the Golestan cancer registry dataset to add complementary data (e.g., survival data). Results: The GoCB started collection of data and biological samples in December 2016. By November 2020, a total number of 1217 cancer patients participated in the GoCB. The majority of the GoCB participants (n = 942, 77%) were those with gastrointestinal and breast cancers. Data on risk factors were successfully collected in 684 (56.2%) of the participants. Overall, 3563 samples were collected from the GoCB participants and 730 samples were used in 7 national and international research projects. Conclusion: We considered specific strategies to overcome major limitations, especially budget shortage, in the development and maintenance of a cancer-specific biological repositories in our setting. The GoCB may be considered as a model for the development of biobank in low-and middle-income countries (LMICs).

Frontiers in Cellular Neuroscience, 2021

Graphical 1Covid-19 triggers endothelial cell (EC) senescence and dysfunction, likely predisposin... more Graphical 1Covid-19 triggers endothelial cell (EC) senescence and dysfunction, likely predisposing to PTSD by increasing microvascular permeability that enables the extravasation of stress molecules into the brain trauma-processing networks in amygdala, hippocampus and the medial prefrontal cortex. The virus upregulates host angiotensin II (ANG II) (via S1 antigen), usurps furin/plasmin (via S2 antigen), mitochondria (via ORF9b), and Sigma-1 receptors (Sig-1Rs) via NSP6. These structures, previously associated with PTSD, link the SARS-CoV-2 virus to increased susceptibility for stress related disorders. As ECs are major producers of brain derived neurotrophic factor (BDNF), a neurotrophin altered in PTSD, senescent ECs lower this molecule further, predisposing to stress related disorders.

Biopreservation and Biobanking, 2021

Biopreservation and Biobanking, 2018

Biopreservation and Biobanking, 2018

R ecently I (J.V.) visited Shanghai and Changsha, China, for a conference and several other activ... more R ecently I (J.V.) visited Shanghai and Changsha, China, for a conference and several other activities related to Biopreservation and Biobanking and ISBER. First, I was honored, along with ISBER President Zisis Kozlakidis, President-elect David Lewandowski, Andrew Brooks, and others to attend and make presentations at the Biobank Branch, China Medicinal Biotech Association (BBCMBA) conference in Shanghai from March 29 to 31. This was a special 10th anniversary celebration of BBCMBA, attracting wide participation from the Chinese biobanking community and hosted by Professor Hengjun Gao, Head of BBCMBA. More recently, as detailed in an ISBER press release on April 20, ISBER and BBCMBA entered into an affiliation agreement that highlights several joint initiatives:

Oncology, 2020

Background: To date, the clinical characteristics of coronavirus disease 19 (COVID-19)-infected u... more Background: To date, the clinical characteristics of coronavirus disease 19 (COVID-19)-infected urologic cancer patients are unknown. Methods: We have analyzed all patients with prostate cancer undergoing hormonal or chemotherapy treatment and receiving telephone and in person pre-triage between March 1 and 27, 2020, at the Tortora Hospital, Pagani, Italy. Results: Among 72 patients, 48 and 24 were hormone-sensitive (HS) and castration-resistant prostate cancer (CRPC), respectively; 0 HS and 2 (8.3%) CRPC (p < 0.05) were positive for COVID-19. Both patients were receiving LHRH agonist therapy, and 1 patient was receiving enzalutamide. Urgent intensive care unit admission was required due to clinical worsening. Blood tests showed severe lymphopenia, anemia, and an increase in platelets. Retroviral therapy, antibiotics, heparin, and chloroquine were prescribed at the beginning. One patient also received tocilizumab as a salvage treatment. After 3 weeks of hospitalization, the patie...

Biopreservation and Biobanking, 2021

Biopreservation and Biobanking, 2020

PLOS ONE, 2020

Background Since the first cases reported in Wuhan, China, in December 2019, the Severe Acute Res... more Background Since the first cases reported in Wuhan, China, in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide. In Indonesia, the first case was reported in early March 2020, and the numbers of confirmed infections have been increasing until now. Efforts to contain the virus globally and in Indonesia are ongoing. This is the very first manuscript using a spatial-temporal model to describe the SARS-CoV-2 transmission in Indonesia, as well as providing a patient profile for all confirmed COVID-19 cases. Method Data was collected from the official website of the Indonesia National Task Force for the Acceleration of COVID-19, from the period of 02 March 2020–02 August 2020. The data from RT-PCR confirmed, SARS-CoV-2 positive patients was categorized according to demographics, symptoms and comorbidities based on case categorization (confirmed, recovered, dead). The data collected provides granular and thorough information on time and ge...

The Lancet Global Health, 2020

Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on ... more Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre-including this research content-immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

Frontiers in Medicine, 2020

Cybergeo, 2009

... Matthieu Ghilardi, Éric Fouache, George Syrides, Mike Styllas, Stathis Stiros et Zisis Kozlak... more ... Matthieu Ghilardi, Éric Fouache, George Syrides, Mike Styllas, Stathis Stiros et Zisis Kozlakidis. ... à proximité du trait de côte, à l'arrière d'un cordon littoral sableux où subsistent des lagunes ; c'est la vision que nous propose Leake (1835, figure 3). Félix de Beaujour vante à ce ...

International Journal of Molecular Sciences

Long COVID, also called post-acute sequelae of SARS-CoV-2, is characterized by a multitude of lin... more Long COVID, also called post-acute sequelae of SARS-CoV-2, is characterized by a multitude of lingering symptoms, including impaired cognition, that can last for many months. This symptom, often called “brain fog”, affects the life quality of numerous individuals, increasing medical complications as well as healthcare expenditures. The etiopathogenesis of SARS-CoV-2-induced cognitive deficit is unclear, but the most likely cause is chronic inflammation maintained by a viral remnant thriving in select body reservoirs. These viral sanctuaries are likely comprised of fused, senescent cells, including microglia and astrocytes, that the pathogen can convert into neurotoxic phenotypes. Moreover, as the enteric nervous system contains neurons and glia, the virus likely lingers in the gastrointestinal tract as well, accounting for the intestinal symptoms of long COVID. Fusogens are proteins that can overcome the repulsive forces between cell membranes, allowing the virus to coalesce with ho...

Open Research Europe

The exposome is a complex scientific field that has enjoyed consistent growth over the last two d... more The exposome is a complex scientific field that has enjoyed consistent growth over the last two decades, defined as the composite of every exposure to which an individual is subjected from conception to death. The study of the exposome requires consideration of both the nature of those exposures and their changes over time, and as such necessitates high quality data and software solutions. As the exposome is both a broad and a recent concept, it is challenging to define or to introduce in a structured way. Thus, an approach to assist with clear definitions and a structured framework is needed for the wider scientific and public communication. Results: A set of 14 personas were developed through three focus groups and a series of 14 semi-structured interviews. The focus groups defined the broad themes specific to exposome research, while the sub-themes emerged to saturation via the interviews process. Personas are imaginary individuals that represent segments/groups of real people wi...

Applied Microbiology

Gut microbes are immunologically tolerated in the gastrointestinal tract but trigger aggressive i... more Gut microbes are immunologically tolerated in the gastrointestinal tract but trigger aggressive immune responses upon translocation across the gut barrier. Although oral tolerance, a physiological process that dampens immune responses to food proteins and commensal microbiota, remains poorly defined, significant progress was made during and after the Human Immunodeficiency Virus epidemic in the 1980s and the discovery of regulatory T cells in 1995. Additional insight was gained after the discoveries of innate lymphoid cells in 2008 and the functional elucidation of mucosal mast cells. Prior to the historical discovery of human pathogens, the etiologies of most human diseases were considered unknown. The same was true about many genetic disorders prior to the Human Genome Project. Here, we hypothesize that many of the remaining idiopathic conditions, including autoimmune, fibroproliferative, and neuropsychiatric diseases as well as some cancers, can be considered microbial translocat...

Biopreservation and Biobanking

Innovations in Digital Health, Diagnostics, and Biomarkers

Biobanks are foundational infrastructures that collect and provide standardized, high-quality, an... more Biobanks are foundational infrastructures that collect and provide standardized, high-quality, and research-ready biological material and associated data. The advent of high-throughput technologies has further supported the creation of biobanks globally. However, the same rate of growth has not occurred in the field of infectious diseases, where biobanks are significantly fewer as compared to other scientific fields such as oncology. This narrative review presents the main aspects that need to be taken into consideration for the construction and application of biological sample infrastructure for infectious diseases, with a number of examples taken from the recent COVID-19 pandemic.

Reports

Infection with SARS-CoV-2, the causative agent of the COVID-19 pandemic, originated in China and ... more Infection with SARS-CoV-2, the causative agent of the COVID-19 pandemic, originated in China and quickly spread across the globe. Despite tremendous economic and healthcare devastation, research on this virus has contributed to a better understanding of numerous molecular pathways, including those involving γ-aminobutyric acid (GABA), that will positively impact medical science, including neuropsychiatry, in the post-pandemic era. SARS-CoV-2 primarily enters the host cells through the renin–angiotensin system’s component named angiotensin-converting enzyme-2 (ACE-2). Among its many functions, this protein upregulates GABA, protecting not only the central nervous system but also the endothelia, the pancreas, and the gut microbiota. SARS-CoV-2 binding to ACE-2 usurps the neuronal and non-neuronal GABAergic systems, contributing to the high comorbidity of neuropsychiatric illness with gut dysbiosis and endothelial and metabolic dysfunctions. In this perspective article, we take a close...

Archives of Iranian Medicine, 2021

Background: We aim to present the development and the initial results of the Golestan Cancer Biob... more Background: We aim to present the development and the initial results of the Golestan Cancer Biobank (GoCB), in a low resource setting in northern Iran. Methods: The GoCB protocol and its standard operation procedures (SOP) were developed according to internationally accepted standards and protocols with some modifications considering the limited resources in our setting. The main biological samples collected by the GoCB include blood sample, urine sample, fresh endoscopy tissue sample, fresh surgical tissue sample and formalin fixed paraffin embedded (FFPE) tissue sample. The GoCB collects patients' demographic data, tumor characteristics as well as data on risk factors. We developed a specific GoCB software for management of patient data and biological sample information. The GoCB dataset is annually linked with the Golestan cancer registry dataset to add complementary data (e.g., survival data). Results: The GoCB started collection of data and biological samples in December 2016. By November 2020, a total number of 1217 cancer patients participated in the GoCB. The majority of the GoCB participants (n = 942, 77%) were those with gastrointestinal and breast cancers. Data on risk factors were successfully collected in 684 (56.2%) of the participants. Overall, 3563 samples were collected from the GoCB participants and 730 samples were used in 7 national and international research projects. Conclusion: We considered specific strategies to overcome major limitations, especially budget shortage, in the development and maintenance of a cancer-specific biological repositories in our setting. The GoCB may be considered as a model for the development of biobank in low-and middle-income countries (LMICs).

Frontiers in Cellular Neuroscience, 2021

Graphical 1Covid-19 triggers endothelial cell (EC) senescence and dysfunction, likely predisposin... more Graphical 1Covid-19 triggers endothelial cell (EC) senescence and dysfunction, likely predisposing to PTSD by increasing microvascular permeability that enables the extravasation of stress molecules into the brain trauma-processing networks in amygdala, hippocampus and the medial prefrontal cortex. The virus upregulates host angiotensin II (ANG II) (via S1 antigen), usurps furin/plasmin (via S2 antigen), mitochondria (via ORF9b), and Sigma-1 receptors (Sig-1Rs) via NSP6. These structures, previously associated with PTSD, link the SARS-CoV-2 virus to increased susceptibility for stress related disorders. As ECs are major producers of brain derived neurotrophic factor (BDNF), a neurotrophin altered in PTSD, senescent ECs lower this molecule further, predisposing to stress related disorders.

Biopreservation and Biobanking, 2021

Biopreservation and Biobanking, 2018

Biopreservation and Biobanking, 2018

R ecently I (J.V.) visited Shanghai and Changsha, China, for a conference and several other activ... more R ecently I (J.V.) visited Shanghai and Changsha, China, for a conference and several other activities related to Biopreservation and Biobanking and ISBER. First, I was honored, along with ISBER President Zisis Kozlakidis, President-elect David Lewandowski, Andrew Brooks, and others to attend and make presentations at the Biobank Branch, China Medicinal Biotech Association (BBCMBA) conference in Shanghai from March 29 to 31. This was a special 10th anniversary celebration of BBCMBA, attracting wide participation from the Chinese biobanking community and hosted by Professor Hengjun Gao, Head of BBCMBA. More recently, as detailed in an ISBER press release on April 20, ISBER and BBCMBA entered into an affiliation agreement that highlights several joint initiatives:

Oncology, 2020

Background: To date, the clinical characteristics of coronavirus disease 19 (COVID-19)-infected u... more Background: To date, the clinical characteristics of coronavirus disease 19 (COVID-19)-infected urologic cancer patients are unknown. Methods: We have analyzed all patients with prostate cancer undergoing hormonal or chemotherapy treatment and receiving telephone and in person pre-triage between March 1 and 27, 2020, at the Tortora Hospital, Pagani, Italy. Results: Among 72 patients, 48 and 24 were hormone-sensitive (HS) and castration-resistant prostate cancer (CRPC), respectively; 0 HS and 2 (8.3%) CRPC (p < 0.05) were positive for COVID-19. Both patients were receiving LHRH agonist therapy, and 1 patient was receiving enzalutamide. Urgent intensive care unit admission was required due to clinical worsening. Blood tests showed severe lymphopenia, anemia, and an increase in platelets. Retroviral therapy, antibiotics, heparin, and chloroquine were prescribed at the beginning. One patient also received tocilizumab as a salvage treatment. After 3 weeks of hospitalization, the patie...

Biopreservation and Biobanking, 2021

Biopreservation and Biobanking, 2020

PLOS ONE, 2020

Background Since the first cases reported in Wuhan, China, in December 2019, the Severe Acute Res... more Background Since the first cases reported in Wuhan, China, in December 2019, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide. In Indonesia, the first case was reported in early March 2020, and the numbers of confirmed infections have been increasing until now. Efforts to contain the virus globally and in Indonesia are ongoing. This is the very first manuscript using a spatial-temporal model to describe the SARS-CoV-2 transmission in Indonesia, as well as providing a patient profile for all confirmed COVID-19 cases. Method Data was collected from the official website of the Indonesia National Task Force for the Acceleration of COVID-19, from the period of 02 March 2020–02 August 2020. The data from RT-PCR confirmed, SARS-CoV-2 positive patients was categorized according to demographics, symptoms and comorbidities based on case categorization (confirmed, recovered, dead). The data collected provides granular and thorough information on time and ge...

The Lancet Global Health, 2020

Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on ... more Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre-including this research content-immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

Frontiers in Medicine, 2020