Zoltan Deim - Academia.edu (original) (raw)
Papers by Zoltan Deim
PubMed, Jul 1, 2007
The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted b... more The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted bovine fetuses. Polymerase chain reaction (PCR) and in situ DNA hybridization proved the presence of BoHV-4 DNA in 7 (29%) of the fetuses. The BoHV-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic Kupffer cells, in all 7 PCR-positive fetuses. However, BoHV-4-specific monoclonal antibody failed to detect viral antigen in the formalin-fixed, paraffin-embedded tissue samples. No bacterial pathogens were found in the tissues of the BoHV-4-positive fetuses. Fungi were detected in 1 sample, and antibody to bovine viral diarrhea virus was detected in another. These results indicate that BoHV-4 could play a role in reproductive disorders of cattle, including abortion.
Frontiers in Microbiology, Dec 2, 2021
Phage-Host Coevolution in Pseudomonas aeruginosa and in vivo studies indicated that combined use ... more Phage-Host Coevolution in Pseudomonas aeruginosa and in vivo studies indicated that combined use of PIAS and antibiotics could effectively inhibit P. aeruginosa growth. The phage can either eradicate bacteria or induce antibiotic sensitivity in MDR-resistant clinical strains. We have explored the potential use of combination therapy as an alternative approach against MDR P. aeruginosa infection.
Acta Veterinaria Hungarica, Mar 1, 2008
Two cases of feline vaccine-associated fibrosarcoma (FVAF) are reported. The excised tumours were... more Two cases of feline vaccine-associated fibrosarcoma (FVAF) are reported. The excised tumours were both characterised as well circumscribed, subcutaneous, firm and white with central necrosis. Histopathologically, they consisted of well-differentiated and variably sized and shaped anaplastic cells, characterised by marked nuclear and cellular pleomorphism including giant cells. The mitotic activity was low. Aluminium was demonstrated in the central necrosis and giant cells. Neoplastic cells were positive for vimentin and negative for desmin and cytokeratin. The presence of feline sarcoma virus and feline immunodeficiency virus could not be detected by PCR in either case.
Avian Pathology, May 11, 2007
Veterinary Record, Jun 1, 2006
ADVENTITIOUS bursitis of the hock is reportedly a common abnormality in pigs (Penny and Hill 1974... more ADVENTITIOUS bursitis of the hock is reportedly a common abnormality in pigs (Penny and Hill 1974). The adventitious bursa is a fluid-filled sac that develops in the subcutaneous connective tissue (Adams 1974). It is not connected to any joint, joint cavity or tendon (Backstrom and Henricson 1966). Some authors have found a significant correlation between the occurrence of adventitious bursitis and the type of floor on which pigs are housed: fully slatted floors have been found to increase the prevalence of the disorder compared with bare solid concrete floors (84 per cent v 20 per cent) (Mouttotou and others 1998). There is no evidence to support the association of this disorder with bone or joint abnormalities (Orsi 1967). Mycoplasma hyorhinis has been isolated from adventitious bursas in fiveand 11-week-old pigs (Nielsen 1988), but the involvement of live pathogens has not been indicated in the majority of reports. A similar disease, the so-called ‘cystic adventitial disease of the popliteal artery’, has been described in human medicine (Scobie and Curry 1975). In this condition, durable strain, microtrauma and intermittent claudication are all frequent factors in the history (Shute and Rothnie 1973). Ultrasonography shows the adventitial cyst surrounding the popliteal artery as a distinct cuff, slightly compressing the artery, and a wall of fibroconnective tissue composed of compact collagen interspersed with strands of elastin fibres (di Marzo and others 1994, Sys and others 1997). This short communication describes cases of adventitious bursitis in a herd of 500 sows on a farrow-to-finish unit in north-west Hungary in February 2004. The herd had both Seghers and Large White genetics. Replacement gilts were selected and reared on-site. The bursitis had a prevalence of 70 per cent in the nursery (piglets up to 28 days old), 74 per cent in the grower phase (from 28 to 80 days old) and 97·5 per cent in finishers (from 80 to approximately 180 to 200 days old). Suckling piglets and weaned pigs were reared on plastic slatted floors, and finishing pigs were kept on bare solid concrete floors without bedding. The affected pigs were reluctant to stand up and move; they ‘toddled’ and generally spent more time than usual at the feeders after eating. The lesions of adventitious bursitis were lateral and lateroplantar distal to the tarsal joint; they were 1 to 6 cm in diameter and had a fluctuant feeling on palpation (Fig 1a). The skin above the lesions became thickened, but no discoloration or ulcerations were observed. There were no postural abnormalities and no other foot lesions. Three affected bursas, among 25 carcases, were found associated with the adventitia of the lateral saphenous vein (Fig 1b). The bursas would be removed in their entirety without injuring the vascular wall. There was no association of the adventitious bursa with the subcutaneous bursa of the tarsal joint or the subtendineus bursa, neither of which showed any lesions. The adventitious bursas were fluid-filled sacs, lined with brownish-red, chronic granulation tissue. Aerobic and anaerobic bacterial cultures of the bursal fluids and walls yielded negative results. Mycoplasma and Chlamydia species were not detected by specific PCR, by immunohistochemistry or by transmission electron microscopy. Histopathological examination revealed both acute-subacute and chronic lesions. The bursal walls were lined with synovial-like cells. The outer layer of the wall of the acute-subacute lesions contained angiofibroblast tissue proliferation, which was continuous with the adventitia of the saphenous vein, together with lymphohistiFIG 1: Lateral and lateroplantar lesions of adventitious bursitis in pigs. (a) Bursa observed under the tarsal joint (arrow) during physical examination. (b) On dissection, the bursa was found to be associated with the saphenous vein (VS). The division of the vein into cranial (VCR) and caudal (VCD) branches are exposed and indicated by dotted lines.
Emerging Infectious Diseases, Dec 1, 2022
A, et al. Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interfac... more A, et al. Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis. Sci Rep.
Canadian Journal of Infectious Diseases & Medical Microbiology, Apr 4, 2019
Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to ... more Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.
PubMed, Aug 1, 2007
T umors of chemoreceptor organs are called chemodectomas or paragangliomas. These chemoreceptor o... more T umors of chemoreceptor organs are called chemodectomas or paragangliomas. These chemoreceptor organs are responsible for detecting changes in blood oxygen and carbon dioxide levels, and in blood pH. The aortic and carotid bodies are the most common sites for the development of paragangliomas in dogs (1,2) and cats (3). The aortic body is located in the aortic arch at the bifurcation of the subclavian artery and the carotic body is located at the bifurcation of the common carotid artery. Aortic body tumors are reported to occur 4 to 5 times more frequently than carotid body tumors in dogs (2,4). These tumors usually express neuroendocrine markers that can be detected by using immunohistochemical staining, such as neuron specific enolase (NSE), synaptophysin, and chromogranin A (4-6). Ultrastructurally, the cytoplasm of the tumor cells contains neurosecretory dense core granules (7). Many carotid body tumors are malignant, in contrast to aortic body tumors (8), and occasionally metastasize to regional lymph nodes, lung, liver, pancreas, bone, and kidney (9-11). We diagnosed a carotid body tumor in an 8-year-old, female, white puli dog. The owners observed that the dog became moody and inactive, and developed hoarseness. The dog was given long-acting antibiotics and a combined vitamin (A 1 D 3 1 E) injection. After this treatment, the dog's physical status slightly improved. The dog was returned to the clinic after 37 d with the following signs: distress, hyperpnea, dyspnea, increased intraocular pressure (glaucoma) of the left eye, and bilateral dilation of the pupils. At the beginning of the clinical examination, the dog collapsed and was given oxygen after intubation. On physical examination, an extensive, firm mass surrounding the larynx was found, but the parotid, mandibular, and the retropharyngeal lymph nodes were normal. The owners opted for euthanasia. Necropsy disclosed a 105-3 30-mm, firm, and multilobulated tumor in the left lateral laryngeal region (Figure 1). The swelling extended to the Y-shaped fusion of the linguofacial and maxillary veins forming the external jugular vein, but it was not associated with these veins. Medial to this branching, the tumor masked the bifurcation of the common carotid artery,
Veterinary Record, Jun 1, 2006
INCLUSION body rhinitis in pigs, caused by porcine cytomegalovirus (PCMV), occurs sporadically wo... more INCLUSION body rhinitis in pigs, caused by porcine cytomegalovirus (PCMV), occurs sporadically worldwide (Done 1955, Harding 1958, Edington 1999). The virus belongs to the Betaherpesvirinae family, and although it is species specific, it shares homologies with the cyto megaloviruses of human beings and other animals (Edington 1999). PCMV infection remains mostly inapparent in older pigs, but infection of the dam during gestation can lead to fetal death and mummification, abortion, low viability of piglets at birth and consequential preweaning mortality (Edington and others 1988). Infection in threeto five-week-old piglets may occur under unfavourable conditions, with clinical signs of sneezing, nasal discharge and respiratory distress, and may even result in 20 to 25 per cent mortality. In growers, the immunosuppressive effect of the virus can lead to exacerbation of certain diseases in the herd. The virus is also thought to contribute to pneumonia in growers. Infection can be diagnosed by the presence of characteristic large, basophilic, intranuclear inclusion bodies in cytomegalic cells of the nasal glandular epithelium. Such inclusion bodies and mononuclear cellular infiltration can also be detected in the tubular epithelia of the kidneys, as well as the epithelia of the salivary and tear glands. Other, less frequent, lesions include interstitial pneumonia and lymphocytic perivasculitis in the brain (Kelly 1967, Edington and others 1976). Predisposing factors of the infection are not fully known. A low level of immunity within the herd (for example, in newly established herds) and immunosuppressive effects may play a role (Edington 1999). Seroconversion due to PCMV is probably much more frequent than clinical disease (Lim and others 2002). A PCR and nested PCR have recently been developed for the specific detection of PCMV nucleic acid (Hamel and others 1999, Lim and others 2002). This short communication describes inclusion body rhinitis in pigs in Hungary for the first time. From mid-December 2002, severe losses occurred among sucking piglets in a herd of 350 farrow-to-finish pigs in southern Hungary. Piglets with apparently normal vigour at birth died without clinical signs at two to three days of age; a similar problem was observed later in twoto three-weekold piglets. Total piglet mortality in the farrowing houses approached 40 per cent at its worst, with several episodes of high mortality observed between mid-December 2002 and mid-April 2003. Sow mortality was low and unaffected, and the incidence of postparturient hypogalactia syndrome and/or discharges after farrowing did not increase. Litters from primiparous and older sows were equally affected. The number of stillbirths per litter rose slightly, but the number of mummified piglets per litter was unaffected. There was a decrease in farrowing rate of approximately 10 per cent starting from mid-September 2002 and ending in March 2003. Pregnancy losses were attributed to an increase in both regular and irregular repeats and the number of sows ‘not in pig’. Almost the whole herd of breeding sows had to be replaced. Replacement breeding gilts were purchased from the original source, which did not display any clinical signs of infection at that time. Unfortunately, there was no close veterinary supervision when the problem started, so diagnostic efforts were not initiated until the end of March 2003. FIG 1: (a) Cytomegaly and large basophilic inclusion body formation in glandular epithelial cells (arrow). Haematoxylin and eosin. x 100. (b) Porcine cytomegalovirus (arrow) in glandular epithelial cells. Transmission electron microscopy. x 25,000
Pathogens, 2021
In the last couple of years, the implementation of long-read sequencing (LRS) technologies for tr... more In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencing with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5′-truncations of larger putative mRNAs being 3′-co-terminal with canonical mRNAs of PRV. A large fraction of these putative RNAs contain in-frame ATGs, which might initiate translation of N-terminally truncated polypeptides. Our analyses indicate that CTO-...
Journal of sports science & medicine, 2018
Cardiovascular morbidity and mortality of premenopausal women are significantly lower compared to... more Cardiovascular morbidity and mortality of premenopausal women are significantly lower compared to men of similar age. However, this protective effect evidently decreases after the onset of menopause. We hypothesized that physical exercise could be a potential therapeutic strategy to improve inflammatory processes and cardiovascular antioxidant homeostasis, which can be affected by the loss of estrogen and the adverse environmental factors, such as overnutrition. Ovariectomized (OVX, n= 40) and sham-operated (SO, n= 40) female Wistar rats were randomized to exercising (R) and non-exercising (NR) groups. Feeding parameters were chosen to make a standard chow (CTRL) or a high triglyceride diet (HT) for 12 weeks. Aortic and cardiac heme oxygenase (HO) activity and HO-1 concentrations significantly decreased in all of the NR OVX and SO HT groups. However, the 12-week physical exercise was found to improve HO-1 values. Plasma IL-6 concentrations were higher in the NR OVX animals and rats ...
In the last couple of years, the implementation of long-read sequencing (LRS) technologies for tr... more In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencings with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5’-truncated putative mRNAs embedded into larger host mRNAs. A large fraction of these RNA molecules contain in-frame ORFs, which may encode N-terminally truncated polypeptides. These study demonstrates that the PRV transcriptome is much more complex than ...
Veterinary Record, 2019
Porcine circovirus 3 (PCV3) infection has been reported in piglets and sows with porcine dermatit... more Porcine circovirus 3 (PCV3) infection has been reported in piglets and sows with porcine dermatitis and nephropathy syndrome, reproductive failure, and cardiac and multisystemic inflammation. Few studies linked PCV3 infection to increased incidence of abortion and weak‐born piglets. This is the first report of a detection of PCV3 Hungarian strain in several organs of aborted and weak‐born piglets, including the thymus, lymph node, placenta, spleen, kidney and the liver. The tissue tropism of PCV3 in affected litters was analysed using real‐time quantitative PCR, and the result showed the highest load of viral DNA in the thymus and lymph nodes. The ORF2 of Hungarian PCV3 strains was 524 nucleotides in length, and the sequence identity to GenBank sequences ranged from 98.5 per cent to 99.2 per cent. The results suggest that PCV3 may have a relevant role in reproductive failure in gilts.
Magyar Allatorvosok Lapja
Acta Veterinaria Hungarica, 2007
Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning mul... more Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). This report describes the first detection of PCV2 and associated diseases in a Romanian swine herd located in Transylvania. The clinical signs, pathological and histopathological changes observed in affected pigs were similar to those previously described for PDNS and PMWS. Polymerase chain reaction and in situ hybridisation were used for the detection of PCV2 nucleic acids from tissues and serum samples. Complete PCV2 genomes of both PMWS and PDNS cases were sequenced and analysed, and by comparing them with each other no genomic differences could be detected. The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary.
PubMed, Jul 1, 2007
The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted b... more The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted bovine fetuses. Polymerase chain reaction (PCR) and in situ DNA hybridization proved the presence of BoHV-4 DNA in 7 (29%) of the fetuses. The BoHV-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic Kupffer cells, in all 7 PCR-positive fetuses. However, BoHV-4-specific monoclonal antibody failed to detect viral antigen in the formalin-fixed, paraffin-embedded tissue samples. No bacterial pathogens were found in the tissues of the BoHV-4-positive fetuses. Fungi were detected in 1 sample, and antibody to bovine viral diarrhea virus was detected in another. These results indicate that BoHV-4 could play a role in reproductive disorders of cattle, including abortion.
Frontiers in Microbiology, Dec 2, 2021
Phage-Host Coevolution in Pseudomonas aeruginosa and in vivo studies indicated that combined use ... more Phage-Host Coevolution in Pseudomonas aeruginosa and in vivo studies indicated that combined use of PIAS and antibiotics could effectively inhibit P. aeruginosa growth. The phage can either eradicate bacteria or induce antibiotic sensitivity in MDR-resistant clinical strains. We have explored the potential use of combination therapy as an alternative approach against MDR P. aeruginosa infection.
Acta Veterinaria Hungarica, Mar 1, 2008
Two cases of feline vaccine-associated fibrosarcoma (FVAF) are reported. The excised tumours were... more Two cases of feline vaccine-associated fibrosarcoma (FVAF) are reported. The excised tumours were both characterised as well circumscribed, subcutaneous, firm and white with central necrosis. Histopathologically, they consisted of well-differentiated and variably sized and shaped anaplastic cells, characterised by marked nuclear and cellular pleomorphism including giant cells. The mitotic activity was low. Aluminium was demonstrated in the central necrosis and giant cells. Neoplastic cells were positive for vimentin and negative for desmin and cytokeratin. The presence of feline sarcoma virus and feline immunodeficiency virus could not be detected by PCR in either case.
Avian Pathology, May 11, 2007
Veterinary Record, Jun 1, 2006
ADVENTITIOUS bursitis of the hock is reportedly a common abnormality in pigs (Penny and Hill 1974... more ADVENTITIOUS bursitis of the hock is reportedly a common abnormality in pigs (Penny and Hill 1974). The adventitious bursa is a fluid-filled sac that develops in the subcutaneous connective tissue (Adams 1974). It is not connected to any joint, joint cavity or tendon (Backstrom and Henricson 1966). Some authors have found a significant correlation between the occurrence of adventitious bursitis and the type of floor on which pigs are housed: fully slatted floors have been found to increase the prevalence of the disorder compared with bare solid concrete floors (84 per cent v 20 per cent) (Mouttotou and others 1998). There is no evidence to support the association of this disorder with bone or joint abnormalities (Orsi 1967). Mycoplasma hyorhinis has been isolated from adventitious bursas in fiveand 11-week-old pigs (Nielsen 1988), but the involvement of live pathogens has not been indicated in the majority of reports. A similar disease, the so-called ‘cystic adventitial disease of the popliteal artery’, has been described in human medicine (Scobie and Curry 1975). In this condition, durable strain, microtrauma and intermittent claudication are all frequent factors in the history (Shute and Rothnie 1973). Ultrasonography shows the adventitial cyst surrounding the popliteal artery as a distinct cuff, slightly compressing the artery, and a wall of fibroconnective tissue composed of compact collagen interspersed with strands of elastin fibres (di Marzo and others 1994, Sys and others 1997). This short communication describes cases of adventitious bursitis in a herd of 500 sows on a farrow-to-finish unit in north-west Hungary in February 2004. The herd had both Seghers and Large White genetics. Replacement gilts were selected and reared on-site. The bursitis had a prevalence of 70 per cent in the nursery (piglets up to 28 days old), 74 per cent in the grower phase (from 28 to 80 days old) and 97·5 per cent in finishers (from 80 to approximately 180 to 200 days old). Suckling piglets and weaned pigs were reared on plastic slatted floors, and finishing pigs were kept on bare solid concrete floors without bedding. The affected pigs were reluctant to stand up and move; they ‘toddled’ and generally spent more time than usual at the feeders after eating. The lesions of adventitious bursitis were lateral and lateroplantar distal to the tarsal joint; they were 1 to 6 cm in diameter and had a fluctuant feeling on palpation (Fig 1a). The skin above the lesions became thickened, but no discoloration or ulcerations were observed. There were no postural abnormalities and no other foot lesions. Three affected bursas, among 25 carcases, were found associated with the adventitia of the lateral saphenous vein (Fig 1b). The bursas would be removed in their entirety without injuring the vascular wall. There was no association of the adventitious bursa with the subcutaneous bursa of the tarsal joint or the subtendineus bursa, neither of which showed any lesions. The adventitious bursas were fluid-filled sacs, lined with brownish-red, chronic granulation tissue. Aerobic and anaerobic bacterial cultures of the bursal fluids and walls yielded negative results. Mycoplasma and Chlamydia species were not detected by specific PCR, by immunohistochemistry or by transmission electron microscopy. Histopathological examination revealed both acute-subacute and chronic lesions. The bursal walls were lined with synovial-like cells. The outer layer of the wall of the acute-subacute lesions contained angiofibroblast tissue proliferation, which was continuous with the adventitia of the saphenous vein, together with lymphohistiFIG 1: Lateral and lateroplantar lesions of adventitious bursitis in pigs. (a) Bursa observed under the tarsal joint (arrow) during physical examination. (b) On dissection, the bursa was found to be associated with the saphenous vein (VS). The division of the vein into cranial (VCR) and caudal (VCD) branches are exposed and indicated by dotted lines.
Emerging Infectious Diseases, Dec 1, 2022
A, et al. Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interfac... more A, et al. Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis. Sci Rep.
Canadian Journal of Infectious Diseases & Medical Microbiology, Apr 4, 2019
Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to ... more Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.
PubMed, Aug 1, 2007
T umors of chemoreceptor organs are called chemodectomas or paragangliomas. These chemoreceptor o... more T umors of chemoreceptor organs are called chemodectomas or paragangliomas. These chemoreceptor organs are responsible for detecting changes in blood oxygen and carbon dioxide levels, and in blood pH. The aortic and carotid bodies are the most common sites for the development of paragangliomas in dogs (1,2) and cats (3). The aortic body is located in the aortic arch at the bifurcation of the subclavian artery and the carotic body is located at the bifurcation of the common carotid artery. Aortic body tumors are reported to occur 4 to 5 times more frequently than carotid body tumors in dogs (2,4). These tumors usually express neuroendocrine markers that can be detected by using immunohistochemical staining, such as neuron specific enolase (NSE), synaptophysin, and chromogranin A (4-6). Ultrastructurally, the cytoplasm of the tumor cells contains neurosecretory dense core granules (7). Many carotid body tumors are malignant, in contrast to aortic body tumors (8), and occasionally metastasize to regional lymph nodes, lung, liver, pancreas, bone, and kidney (9-11). We diagnosed a carotid body tumor in an 8-year-old, female, white puli dog. The owners observed that the dog became moody and inactive, and developed hoarseness. The dog was given long-acting antibiotics and a combined vitamin (A 1 D 3 1 E) injection. After this treatment, the dog's physical status slightly improved. The dog was returned to the clinic after 37 d with the following signs: distress, hyperpnea, dyspnea, increased intraocular pressure (glaucoma) of the left eye, and bilateral dilation of the pupils. At the beginning of the clinical examination, the dog collapsed and was given oxygen after intubation. On physical examination, an extensive, firm mass surrounding the larynx was found, but the parotid, mandibular, and the retropharyngeal lymph nodes were normal. The owners opted for euthanasia. Necropsy disclosed a 105-3 30-mm, firm, and multilobulated tumor in the left lateral laryngeal region (Figure 1). The swelling extended to the Y-shaped fusion of the linguofacial and maxillary veins forming the external jugular vein, but it was not associated with these veins. Medial to this branching, the tumor masked the bifurcation of the common carotid artery,
Veterinary Record, Jun 1, 2006
INCLUSION body rhinitis in pigs, caused by porcine cytomegalovirus (PCMV), occurs sporadically wo... more INCLUSION body rhinitis in pigs, caused by porcine cytomegalovirus (PCMV), occurs sporadically worldwide (Done 1955, Harding 1958, Edington 1999). The virus belongs to the Betaherpesvirinae family, and although it is species specific, it shares homologies with the cyto megaloviruses of human beings and other animals (Edington 1999). PCMV infection remains mostly inapparent in older pigs, but infection of the dam during gestation can lead to fetal death and mummification, abortion, low viability of piglets at birth and consequential preweaning mortality (Edington and others 1988). Infection in threeto five-week-old piglets may occur under unfavourable conditions, with clinical signs of sneezing, nasal discharge and respiratory distress, and may even result in 20 to 25 per cent mortality. In growers, the immunosuppressive effect of the virus can lead to exacerbation of certain diseases in the herd. The virus is also thought to contribute to pneumonia in growers. Infection can be diagnosed by the presence of characteristic large, basophilic, intranuclear inclusion bodies in cytomegalic cells of the nasal glandular epithelium. Such inclusion bodies and mononuclear cellular infiltration can also be detected in the tubular epithelia of the kidneys, as well as the epithelia of the salivary and tear glands. Other, less frequent, lesions include interstitial pneumonia and lymphocytic perivasculitis in the brain (Kelly 1967, Edington and others 1976). Predisposing factors of the infection are not fully known. A low level of immunity within the herd (for example, in newly established herds) and immunosuppressive effects may play a role (Edington 1999). Seroconversion due to PCMV is probably much more frequent than clinical disease (Lim and others 2002). A PCR and nested PCR have recently been developed for the specific detection of PCMV nucleic acid (Hamel and others 1999, Lim and others 2002). This short communication describes inclusion body rhinitis in pigs in Hungary for the first time. From mid-December 2002, severe losses occurred among sucking piglets in a herd of 350 farrow-to-finish pigs in southern Hungary. Piglets with apparently normal vigour at birth died without clinical signs at two to three days of age; a similar problem was observed later in twoto three-weekold piglets. Total piglet mortality in the farrowing houses approached 40 per cent at its worst, with several episodes of high mortality observed between mid-December 2002 and mid-April 2003. Sow mortality was low and unaffected, and the incidence of postparturient hypogalactia syndrome and/or discharges after farrowing did not increase. Litters from primiparous and older sows were equally affected. The number of stillbirths per litter rose slightly, but the number of mummified piglets per litter was unaffected. There was a decrease in farrowing rate of approximately 10 per cent starting from mid-September 2002 and ending in March 2003. Pregnancy losses were attributed to an increase in both regular and irregular repeats and the number of sows ‘not in pig’. Almost the whole herd of breeding sows had to be replaced. Replacement breeding gilts were purchased from the original source, which did not display any clinical signs of infection at that time. Unfortunately, there was no close veterinary supervision when the problem started, so diagnostic efforts were not initiated until the end of March 2003. FIG 1: (a) Cytomegaly and large basophilic inclusion body formation in glandular epithelial cells (arrow). Haematoxylin and eosin. x 100. (b) Porcine cytomegalovirus (arrow) in glandular epithelial cells. Transmission electron microscopy. x 25,000
Pathogens, 2021
In the last couple of years, the implementation of long-read sequencing (LRS) technologies for tr... more In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencing with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5′-truncations of larger putative mRNAs being 3′-co-terminal with canonical mRNAs of PRV. A large fraction of these putative RNAs contain in-frame ATGs, which might initiate translation of N-terminally truncated polypeptides. Our analyses indicate that CTO-...
Journal of sports science & medicine, 2018
Cardiovascular morbidity and mortality of premenopausal women are significantly lower compared to... more Cardiovascular morbidity and mortality of premenopausal women are significantly lower compared to men of similar age. However, this protective effect evidently decreases after the onset of menopause. We hypothesized that physical exercise could be a potential therapeutic strategy to improve inflammatory processes and cardiovascular antioxidant homeostasis, which can be affected by the loss of estrogen and the adverse environmental factors, such as overnutrition. Ovariectomized (OVX, n= 40) and sham-operated (SO, n= 40) female Wistar rats were randomized to exercising (R) and non-exercising (NR) groups. Feeding parameters were chosen to make a standard chow (CTRL) or a high triglyceride diet (HT) for 12 weeks. Aortic and cardiac heme oxygenase (HO) activity and HO-1 concentrations significantly decreased in all of the NR OVX and SO HT groups. However, the 12-week physical exercise was found to improve HO-1 values. Plasma IL-6 concentrations were higher in the NR OVX animals and rats ...
In the last couple of years, the implementation of long-read sequencing (LRS) technologies for tr... more In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencings with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5’-truncated putative mRNAs embedded into larger host mRNAs. A large fraction of these RNA molecules contain in-frame ORFs, which may encode N-terminally truncated polypeptides. These study demonstrates that the PRV transcriptome is much more complex than ...
Veterinary Record, 2019
Porcine circovirus 3 (PCV3) infection has been reported in piglets and sows with porcine dermatit... more Porcine circovirus 3 (PCV3) infection has been reported in piglets and sows with porcine dermatitis and nephropathy syndrome, reproductive failure, and cardiac and multisystemic inflammation. Few studies linked PCV3 infection to increased incidence of abortion and weak‐born piglets. This is the first report of a detection of PCV3 Hungarian strain in several organs of aborted and weak‐born piglets, including the thymus, lymph node, placenta, spleen, kidney and the liver. The tissue tropism of PCV3 in affected litters was analysed using real‐time quantitative PCR, and the result showed the highest load of viral DNA in the thymus and lymph nodes. The ORF2 of Hungarian PCV3 strains was 524 nucleotides in length, and the sequence identity to GenBank sequences ranged from 98.5 per cent to 99.2 per cent. The results suggest that PCV3 may have a relevant role in reproductive failure in gilts.
Magyar Allatorvosok Lapja
Acta Veterinaria Hungarica, 2007
Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning mul... more Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). This report describes the first detection of PCV2 and associated diseases in a Romanian swine herd located in Transylvania. The clinical signs, pathological and histopathological changes observed in affected pigs were similar to those previously described for PDNS and PMWS. Polymerase chain reaction and in situ hybridisation were used for the detection of PCV2 nucleic acids from tissues and serum samples. Complete PCV2 genomes of both PMWS and PDNS cases were sequenced and analysed, and by comparing them with each other no genomic differences could be detected. The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary.