Zorica Arsova-Sarafinovska - Academia.edu (original) (raw)

Papers by Zorica Arsova-Sarafinovska

Macedonian Pharmaceutical Bulletin

Metrofood-RI Infrastructure for Promoting Metrology in Food and Nutrition will be built on a Hub ... more Metrofood-RI Infrastructure for Promoting Metrology in Food and Nutrition will be built on a Hub and Node model constituted by: a Central Hub in Italy and a network of 18 Countries. Macedonian Node comprises of two Institutions: Institute of Public Health (IJZRSM) and Faculty of Agriculture and Food Science (FASF) named as Joint Research Unit -Metrofood-MK. Metrofood-RI aimed to promote scientific excellence in the field of food quality and safety. The Infrastructure includes both the physical and electronic infrastructure. Concerning the P-RI, the facilities that will be networked include plants and laboratories for Reference Material (RMs), analytical laboratories, experimental Fields/Farms for food production, facilities for food processing, storage and preparation. The following activities will be performed: characterization of new RMs, the organization and management of interlaboratory tests; the development and validation of new analytical methods and devices. Regarding, agrof...

Introduction - Flavonoids are a large group of polyphenolic components possessing benzo-γ-pyronic... more Introduction - Flavonoids are a large group of polyphenolic components possessing benzo-γ-pyronic structure and widely distributed in plants. The chemical nature and the biological activity of flavonoids depends on the structural class to which they belong, the degree of hydroxylation, the degree of polymerization, and the presence of other substituents and bonds. Today, there are large number of herbal preparations containing plant extracts rich in flavonoids used as OTC drugs, and therefore it is necessary to develop methods for controlling and monitoring their quality. Aim - Development and validation of HPLC method for determination of rutin and quercetin in tablets containing 1200 mg dry leaf of Ginkgo biloba. Method - Simple HPLC method with gradient elution (acetonitrile: 0.3% phosphoric acid), flow rate of 1,2 mL/min, column temperature of 25°C and UV detection (rutin at 255 nm, quercetin at 375 nm) of rutin and quercetin in herbal preparation containing dry leaf of Ginkgo b...

Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substan... more Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substance in the orphan drug used for the treatment of hereditary tyrosinemia type I, a rare genetic disease caused by mutations in the gene for the enzyme fumarylacetoacetase. The aim of our study was to develop a simple and accurate RP–HPLC method with UV detection for routine determination of nitisinone in commercially available pharmaceutical forms. The chromatographic separation was achieved on a reversed-phase column Purospher STAR® RP–8 end–capped (150 x 4.6 mm i.d., particle size 5 μm), with a mobile phase consisted of a mixture of acetonitrile and water acidified with o-phosphoric acid with pH adjusted to 3.0, 65:35 (V/V), filtered through 0.45μm nylon filter. The flow rate was kept at 1 mL/min. A diode array detector measured the UV absorbance at 272 nm. The injection volume was 10 μl. The method was validated by a determination of system suitability, specificity, linearity, range, ac...

Drugs with biological origin or biopharmaceuticals are products where the active substance is com... more Drugs with biological origin or biopharmaceuticals are products where the active substance is composed of or derived from living organisms and often represent the crossing of the boundaries of medical science and different fields of research (Walsh, 2003). They can be composed of carbohydrates, nucleic acids, proteins, or complex combinations of these substances, or may be composed from live cells or tissues. Unlike chemically synthesized small molecules that have a well-defined structure and are thoroughly characterized, the pharmaceutical products with biological origin are complex in structure and therefore their full characterization is great achievement. Analytical methodology that is currently in use enables a comprehensive characterization and identification of biopharmaceuticals (determination of molecular weight and particle size, physico-chemical properties, identity, purity and homogeneity, biological activity, immunochemical properties, purity/impurities (if present), pr...

The Institute of Public Health is the first public health institution in the FYROM that has estab... more The Institute of Public Health is the first public health institution in the FYROM that has established Quality Management System according to ISO/IEC 17025. The accreditation has been granted by the National Institute for Accreditation in December 2006. Since then, the maintenance and improvement of the Quality Management System remains a key element in our policy. It is well-known that one of the elements by which laboratories can demonstrate their competence is through participation in Proficiency Testing (PT) activities. Therefore, our laboratory adopted practice to participate in at least one PT scheme relating to each sub-discipline per a year. Since the first accreditation, our laboratory participated in 15 PT schemes, organized by different providers, such as: Muva Kempten Quality Management & Laboratory Services; FAPAS; DRRR Deutsches Referenzbüro für Lebensmittel-Ringversuche und Referenzmaterialien; LGC; Asia Pacific laboratory Accreditation Cooperation etc. The most freq...

The development of radiolabeled small peptide or peptidomimetic ligands can bind platelets and th... more The development of radiolabeled small peptide or peptidomimetic ligands can bind platelets and their specific expressed receptor have been suggested as a new approach to detect the clot location and, more essentially, to determine the age and morphology of the evolving thrombus. This new approach is focused on the use of a series of radiolabeled platelet GPIIb/IIIa receptor antagonists. Tirofiban N-(butylsulfonyl)- 4-O-(4-(4-piperidyl)-L-tyrosine is a non-peptide tyrosine derivate. The aim of the study was to introduce radioactive-labeled tirofiban as a specific imaging agent for acute DVT and to determine the serum concentrations in normotensive male Wister rats with and without deep acute venous thrombosis in order to confirm the animal model of acute venous thrombosis

University *Goce Delcev* Stip, Jun 1, 2015

Makedonsko Farmacevtski Bilten, 2019

Ibandronate sodium (IBN) [(1-hydroxy-3-(methyl pentyl amino) propylidene bisphosphonic acid monos... more Ibandronate sodium (IBN) [(1-hydroxy-3-(methyl pentyl amino) propylidene bisphosphonic acid monosodium monohydrate)] is the sodium salt of ibandronic acid, a synthetic nitrogen-containing bisphosphonate drug. The aim of this study was to develop a sensitive and accurate RP-HPLC method with indirect UV detection for determination of IBN in pharmaceutical formulations. Chromatographic separation was performed on a Waters Bridge C18 reversed-phase column (250 x 4.6 mm I.D.; particle size 5 µm), in an isocratic mode with a mobile phase constituted of 90% buffer: 10% acetonitrile (V/V). The buffer was made using 1.5 mL ortho-phosphoric acid, 990 mg 1-Hexanesulfonic acid sodium salt 98%, 140 mg EDTA in 1000 mL flask diluted with HPLC grade water. The elution was carried out at a flow rate of 1.0 mL minˉ1. A diode array detector measured the UV absorbance at 198 nm, in inverse mode. The method was validated for specificity/selectivity, linearity, LOD, LOQ, accuracy, precision and robustness according to ICH validation guidelines. The limits of detection and quantification were calculated at 0.0163 µg/mL and 0.0495 µg/mL, respectively. The method was effectively used for determination of IBN from commercial tablets and provided good results without any interference from commonly used excipients.

The atomic emission spectrometry with inductively coupled plasma (ICP-AES) tehnique was used for ... more The atomic emission spectrometry with inductively coupled plasma (ICP-AES) tehnique was used for determination of trace elements presence and their contents in Camellia sinensis teas and its tea mixtures. Samples were collected from macedonian market originating from Macedonia, Serbia and Poland. For sample preparation microwave digestion system using HNO3 and H2O2 was applied. The following trace elements: Al,

Macedonian Pharmaceutical Bulletin, 2019

Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substan... more Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substance in the orphan drug used for the treatment of hereditary tyrosinemia type I, a rare genetic disease caused by mutations in the gene for the enzyme fumarylacetoacetase. The aim of our study was to develop a simple and accurate RP–HPLC method with UV detection for routine determination of nitisinone in commercially available pharmaceutical forms. The chromatographic separation was achieved on a reversed-phase column Purospher STAR® RP–8 end–capped (150 x 4.6 mm i.d., particle size 5 μm), with a mobile phase consisted of a mixture of acetonitrile and water acidified with o-phosphoric acid with pH adjusted to 3.0, 65:35 (V/V), filtered through 0.45μm nylon filter. The flow rate was kept at 1 mL/min. A diode array detector measured the UV absorbance at 272 nm. The injection volume was 10 μL. The method was validated by a determination of system suitability, specificity, linearity, range, ac...

Journal of Agriculture and Plant Sciences, 2017

Bioindication abbility of Hypnum cupressiforme and Homolothecium lutescens for determination of a... more Bioindication abbility of Hypnum cupressiforme and Homolothecium lutescens for determination of arsenic distribution in environment .

Petunia (Petunia sp.) and pink dianthus (Dianthus sp.) are important ornamental plants which are ... more Petunia (Petunia sp.) and pink dianthus (Dianthus sp.) are important ornamental plants which are grown on balconies, in flower gardens, parks and other during summer period. Approximately 156 ornamental species are cultivated via tissue culture in different commercial laboratories worldwide. In vitro plant culture is one of the key tools in plant biotechnology that utilizes the totipotency of plant cells. In this research the influence of different concentrations and combinations of BA, GA3, IAA и NAA on meristem buds and cotyledons of petunia and pink dianthus as starting explants was studied. During the research development of starting explants was observed and certain changes were noted. The meristem buds cultivated on MS medium supplemented with certain growth regulators responded with development of callus firstly, and then leaf rosette and shoots. On the other hand, the meristem buds of pink dianthus developed merely shoots. All combinations of growth regulators under this stu...

Prof. Neven Zarkovic 6. Nicotinamide adenine dinucleotide biosynthesis and consumption in dysfunc... more Prof. Neven Zarkovic 6. Nicotinamide adenine dinucleotide biosynthesis and consumption in dysfunctional white adipocytes Author: Prof. Tatjana Ruskovska 7. New insights into the chemistry and functions of Coenzyme Q Author: Prof. Rubin Gulaboski 8. Genetically modified systems to study muscular dystrophies Author: Prof. Darko Bosnakovski 9. Counteracting microbial biofilm formation onto dental materials Author: Prof. Lia Rimondini 10. The endless potential of the umbilical cord blood for investigation, research and treatment Author: Prof d-r Elizabeta Zisovska 11. The cytokinesis-blocked micronucleus assay: Good choice for detection and evaluation of genotoxity in human cells Author: Ass. Misko Milev 12. Multiple predictive model for clinical and subclinical depression in adolescence Author: Prof. Lence Miloseva 13. Molecular Imaging and therapy with radionuclides of prostate cancer Author: Prof. Adriano Duatti 14. Where Nanotechnology and Nanomedicine meet-Applications and Potential Risks Author: Prof. Vaso Taleski 15. The role of molecular cocrystals in drug development Author: Ass. Aleksandar Cvetkovski 16. Drug development based on radiolabeled antibodies

Different forms of tocopherols, together with tocotrienols, are collectively named as vitamin E (... more Different forms of tocopherols, together with tocotrienols, are collectively named as vitamin E (a lipid-soluble vitamin with antioxidation effect in several stages of peroxidation of lipids in vivo) and each possesses different degree of medical, biological and physiochemical significance. Among four tocopherols (α-, β-, γ- and δ-tocopherol), alpha-tocopherol has been reported to possess the highest biological activity. In order to obtain the larger amounts, vitamin E is synthesized as a racemic mixture of D- and L- forms which are optical isomers, unlike a natural tocopherol that is found only in the D-form. Synthetic vitamin E is derived from petroleum products as allracemic alpha tocopherol and its ester form all-racemic alpha tocopheryl acetate (or DL-tocopheryl acetate). The tocopheryl esters are more stable for an extended time of use. In the belief that vitamin E is a natural skin conditioner and humidifier, it is used in cosmetic industry in the preparation of lipsticks, sh...

➢To investigate the contemporary literary knowledge of the pathogenesis of benign prostatic hyper... more ➢To investigate the contemporary literary knowledge of the pathogenesis of benign prostatic hyperplasia.

Скриптата ,,Клиничка фармација и фармакотерапија” е наменета првенствено на студентите на студиск... more Скриптата ,,Клиничка фармација и фармакотерапија” е наменета првенствено на студентите на студиската програма - магистер по фармација, кои го слушаат предметот Клиничка фармација и фармакотерапија на Факултетот за медицински науки, на Универзитетот ,,Гоце Делчев” во Штип. Текстот е претставен во согласност со наставниот план и програма по клиничка фармација и фармакотерапија, а подготвен со цел да им помогне на студентите во совладување на предвидениот материјал и да им го направи учењето полесно и поинтересно. Со помош на овој текст студентите ќе се запознаат со поимот и дефиницијата за клиничка фармација, главната цел и нивоата на дејствување на клиничките фармацевти, нивната улога и задачи во современиот интегриран здравствен систем. Оваа скрипта ќе им овозможи на студентите да ги прошират своите знаења за интеракциите на лекови и механизмите на нивно настанување, да ги препознаваат лековите со висок потенцијал за појава на интеракции, како и групите пациенти со зголемен ризик за...

In the last few decades, capsaicin has exerted potent anti-cancer properties by enouncing anti-pr... more In the last few decades, capsaicin has exerted potent anti-cancer properties by enouncing anti-proliferative, apoptotic and anti-metastatic activity. To the best of our knowledge, the specific cell targets and successive mechanism of cytotoxic activity of capsaicin in different type of malignant cells, still remains unclear and contradictory. Therefore, this mini review, explains some of the most significant chemopreventive mechanisms of the action of capsaicin at a cellular level, reported in the recent literature.

Journal of Agriculture and Plant Sciences, 2017

Abstract Capsaicin is the main compound in the group of protoalkaloids, so called capsaicinoids. ... more Abstract Capsaicin is the main compound in the group of protoalkaloids, so called capsaicinoids. Biosynthesis of capsaicinoids is specific characteristic only for secondary metabolism of the plant metabolism of genus Capsicum. Hot peppers contain vanylil conjugates of amid type, which are absent or switched with their not hot esters or isosters (capsinoids) in the mild types of peppers. The pepper, Capsicum annuum L., fam. Solanaceae, is one of the leading agricultures in Republic of Macedonia. Its fruits have been used for a long time, mainly because of its nutritive value, throught the period of all seasons, in their botanical or technical maturity. Therefore, the aim of this paper was to analyze the percentage ratio of the most common capsaicinoids, present in hot peppers, as: capsaicin, dihydrocapsaicin and nordihydrocapsaicin. In the extracts obtained from the fruits of fifteen different genotypes of Capsicum annuum L

Macedonian Pharmaceutical Bulletin

University *Goce Delcev* Stip, Jun 1, 2015

Makedonsko Farmacevtski Bilten, 2019

Macedonian Pharmaceutical Bulletin, 2019

Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substan... more Nitisinone is a reversible inhibitor of 4-hydroxyphenylpyruvate dioxygenase and an active substance in the orphan drug used for the treatment of hereditary tyrosinemia type I, a rare genetic disease caused by mutations in the gene for the enzyme fumarylacetoacetase. The aim of our study was to develop a simple and accurate RP–HPLC method with UV detection for routine determination of nitisinone in commercially available pharmaceutical forms. The chromatographic separation was achieved on a reversed-phase column Purospher STAR® RP–8 end–capped (150 x 4.6 mm i.d., particle size 5 μm), with a mobile phase consisted of a mixture of acetonitrile and water acidified with o-phosphoric acid with pH adjusted to 3.0, 65:35 (V/V), filtered through 0.45μm nylon filter. The flow rate was kept at 1 mL/min. A diode array detector measured the UV absorbance at 272 nm. The injection volume was 10 μL. The method was validated by a determination of system suitability, specificity, linearity, range, ac...

Journal of Agriculture and Plant Sciences, 2017