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Zsolt Dajnoki

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Papers by Zsolt Dajnoki

Research paper thumbnail of Revealing Barrier Alterations in Rosacea Skin at the Molecular Level

Research paper thumbnail of Examination of peripheral blood dendritic cells in atopic dermatitis

Research paper thumbnail of Immune-mediated skin inflammation is the same atopic dermatitis patients with or without filaggrin mutation

Research paper thumbnail of IFNg/IL-17 cytokine milieu is characteristic of papulopustular rosacea

Research paper thumbnail of Allergen specific immunotherapy in atopic dermatitis

Research paper thumbnail of A bőr immunrendszerének vizsgálata rosaceában

Research paper thumbnail of Investigation of the role of Thymic Stromal Lymphopoietin in healthy skin and in immune-mediated skin inflammation

Research paper thumbnail of Detection of Antimicrobial Peptides in Stratum Corneum by Mass Spectrometry

Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maint... more Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maintenance of skin barrier functions. Here, we developed a novel approach for the examination of AMPs in the outermost layer of the epidermis, namely stratum corneum (SC). The SC sample collection by tape stripping was coupled with detection by highly specific and sensitive parallel reaction monitoring (PRM)-based mass spectrometry. We found that hexane-free processing of SC samples produced higher protein yield compared to hexane-based extraction. Of the 18 investigated peptides, 9 could be detected either in healthy or in inflamed skin specimens. Regarding the amount of S100A8, LCN2, LACRT and LYZ significant topographical differences were described among gland poor (GP), sebaceous gland rich (SGR) and apocrine gland rich (AGR) healthy skin regions. We applied a minimally invasive, reproducible approach for sampling, which can be assessed for research and diagnostic purposes and for monito...

Research paper thumbnail of 227 Primary Changes in the Development of Hidradenitis Suppurativa

Journal of Investigative Dermatology, 2021

Research paper thumbnail of 193 Different Skin Regions are Characterized by Distinct Epimmunome Patterns

Journal of Investigative Dermatology, 2021

Research paper thumbnail of Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases

International Journal of Molecular Sciences, 2021

The chemical milieu, microbiota composition, and immune activity show prominent differences in di... more The chemical milieu, microbiota composition, and immune activity show prominent differences in distinct healthy skin areas. The objective of the current study was to compare the major permeability barrier components (stratum corneum and tight junction (TJ)), investigate the distribution of (corneo)desmosomes and TJs, and measure barrier function in healthy sebaceous gland-rich (SGR), apocrine gland-rich (AGR), and gland-poor (GP) skin regions. Molecules involved in cornified envelope (CE) formation, desquamation, and (corneo)desmosome and TJ organization were investigated at the mRNA and protein levels using qRT-PCR and immunohistochemistry. The distribution of junction structures was visualized using confocal microscopy. Transepidermal water loss (TEWL) functional measurements were also performed. CE intracellular structural components were similarly expressed in gland-rich (SGR and AGR) and GP areas. In contrast, significantly lower extracellular protein levels of (corneo)desmosom...

Research paper thumbnail of Regional variability of melanoma incidence and prevalence in Hungary. Epidemiological impact of ambient UV radiation and socioeconomic factors

European Journal of Cancer Prevention, 2021

Research paper thumbnail of Weak Links in the Permeability Barrier of theSkin

Research paper thumbnail of Permeability and immune barrier differences of healthy human skin

Research paper thumbnail of Novel insights into the TRPV3-mediated itch in atopic dermatitis

Journal of Allergy and Clinical Immunology, 2020

Research paper thumbnail of Comparison of Immune and Barrier Characteristics in Scalp and Skin Psoriasis

Acta Dermato Venereologica, 2020

Research paper thumbnail of Rosacea Is Characterized by a Profoundly Diminished Skin Barrier

Journal of Investigative Dermatology, 2020

Research paper thumbnail of Acne: Transient Arrest in the Homeostatic Host–Microbiota Dialog?

Trends in Immunology, 2019

Research paper thumbnail of 243 Permeability and immune barrier differences of healthy human skin

Journal of Investigative Dermatology, 2019

Adiponectin enhances human keratinocyte lipid synthesis via SIRT1 and nuclear hormone receptor si... more Adiponectin enhances human keratinocyte lipid synthesis via SIRT1 and nuclear hormone receptor signaling. In an effort to put adiponectin signaling into practical use for the improvement of skin barrier function, the effects of a newly synthesized adiponectin-derived peptide (Adi-pep D) on keratinocyte lipid synthesis and differentiation were evaluated in vitro and in vivo. Adi-pep D induced adiponectin receptor activity by receptor-mediated endocytosis in HaCaT cells Further, in Adi-pep D-treated primary human keratinocytes, Adi-pep D increased the expression of the lipid synthesis enzymes HMG CoA reductase and serinepalmitoyl transferase mRNA as well as the lipid transporter ABCA12, which is related to the homeostasis of skin barrier function. The expression levels of the transcription factors SIRT1, LXRa, and SREBP also increased. Nile red fluorescence staining showed an increase in the neutral lipid content in Adi-pep-treated keratinocytes. Adi-pep D also increased the expression of epidermal differentiation marker proteins (e.g., filaggrin, involucrin, and loricrin) in HaCaT cells. We further evaluated whether the synthetic peptide can reduce the deterioration of barrier function by the topical application of a highly potent corticosteroid on murine skin. Functionally, the basal transepidermal waterloss and stratum corneum hydration levels were not different but the barrier recovery rate and integrity tended to be better than those of the non-treated skin. When the epidermis was observed by transmission electron microscopy, the count and secretion of the lamellar body in the stratum corneum-granulosa interface were significantly increased. These results suggest that the newly synthesized Adi-pep can improve lipid synthesis, differentiation, and the recovery of skin barrier function.

Research paper thumbnail of 393 Immune characteristics of scalp and skin psoriasis

Journal of Investigative Dermatology, 2019

Research paper thumbnail of Revealing Barrier Alterations in Rosacea Skin at the Molecular Level

Research paper thumbnail of Examination of peripheral blood dendritic cells in atopic dermatitis

Research paper thumbnail of Immune-mediated skin inflammation is the same atopic dermatitis patients with or without filaggrin mutation

Research paper thumbnail of IFNg/IL-17 cytokine milieu is characteristic of papulopustular rosacea

Research paper thumbnail of Allergen specific immunotherapy in atopic dermatitis

Research paper thumbnail of A bőr immunrendszerének vizsgálata rosaceában

Research paper thumbnail of Investigation of the role of Thymic Stromal Lymphopoietin in healthy skin and in immune-mediated skin inflammation

Research paper thumbnail of Detection of Antimicrobial Peptides in Stratum Corneum by Mass Spectrometry

Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maint... more Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maintenance of skin barrier functions. Here, we developed a novel approach for the examination of AMPs in the outermost layer of the epidermis, namely stratum corneum (SC). The SC sample collection by tape stripping was coupled with detection by highly specific and sensitive parallel reaction monitoring (PRM)-based mass spectrometry. We found that hexane-free processing of SC samples produced higher protein yield compared to hexane-based extraction. Of the 18 investigated peptides, 9 could be detected either in healthy or in inflamed skin specimens. Regarding the amount of S100A8, LCN2, LACRT and LYZ significant topographical differences were described among gland poor (GP), sebaceous gland rich (SGR) and apocrine gland rich (AGR) healthy skin regions. We applied a minimally invasive, reproducible approach for sampling, which can be assessed for research and diagnostic purposes and for monito...

Research paper thumbnail of 227 Primary Changes in the Development of Hidradenitis Suppurativa

Journal of Investigative Dermatology, 2021

Research paper thumbnail of 193 Different Skin Regions are Characterized by Distinct Epimmunome Patterns

Journal of Investigative Dermatology, 2021

Research paper thumbnail of Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases

International Journal of Molecular Sciences, 2021

The chemical milieu, microbiota composition, and immune activity show prominent differences in di... more The chemical milieu, microbiota composition, and immune activity show prominent differences in distinct healthy skin areas. The objective of the current study was to compare the major permeability barrier components (stratum corneum and tight junction (TJ)), investigate the distribution of (corneo)desmosomes and TJs, and measure barrier function in healthy sebaceous gland-rich (SGR), apocrine gland-rich (AGR), and gland-poor (GP) skin regions. Molecules involved in cornified envelope (CE) formation, desquamation, and (corneo)desmosome and TJ organization were investigated at the mRNA and protein levels using qRT-PCR and immunohistochemistry. The distribution of junction structures was visualized using confocal microscopy. Transepidermal water loss (TEWL) functional measurements were also performed. CE intracellular structural components were similarly expressed in gland-rich (SGR and AGR) and GP areas. In contrast, significantly lower extracellular protein levels of (corneo)desmosom...

Research paper thumbnail of Regional variability of melanoma incidence and prevalence in Hungary. Epidemiological impact of ambient UV radiation and socioeconomic factors

European Journal of Cancer Prevention, 2021

Research paper thumbnail of Weak Links in the Permeability Barrier of theSkin

Research paper thumbnail of Permeability and immune barrier differences of healthy human skin

Research paper thumbnail of Novel insights into the TRPV3-mediated itch in atopic dermatitis

Journal of Allergy and Clinical Immunology, 2020

Research paper thumbnail of Comparison of Immune and Barrier Characteristics in Scalp and Skin Psoriasis

Acta Dermato Venereologica, 2020

Research paper thumbnail of Rosacea Is Characterized by a Profoundly Diminished Skin Barrier

Journal of Investigative Dermatology, 2020

Research paper thumbnail of Acne: Transient Arrest in the Homeostatic Host–Microbiota Dialog?

Trends in Immunology, 2019

Research paper thumbnail of 243 Permeability and immune barrier differences of healthy human skin

Journal of Investigative Dermatology, 2019

Adiponectin enhances human keratinocyte lipid synthesis via SIRT1 and nuclear hormone receptor si... more Adiponectin enhances human keratinocyte lipid synthesis via SIRT1 and nuclear hormone receptor signaling. In an effort to put adiponectin signaling into practical use for the improvement of skin barrier function, the effects of a newly synthesized adiponectin-derived peptide (Adi-pep D) on keratinocyte lipid synthesis and differentiation were evaluated in vitro and in vivo. Adi-pep D induced adiponectin receptor activity by receptor-mediated endocytosis in HaCaT cells Further, in Adi-pep D-treated primary human keratinocytes, Adi-pep D increased the expression of the lipid synthesis enzymes HMG CoA reductase and serinepalmitoyl transferase mRNA as well as the lipid transporter ABCA12, which is related to the homeostasis of skin barrier function. The expression levels of the transcription factors SIRT1, LXRa, and SREBP also increased. Nile red fluorescence staining showed an increase in the neutral lipid content in Adi-pep-treated keratinocytes. Adi-pep D also increased the expression of epidermal differentiation marker proteins (e.g., filaggrin, involucrin, and loricrin) in HaCaT cells. We further evaluated whether the synthetic peptide can reduce the deterioration of barrier function by the topical application of a highly potent corticosteroid on murine skin. Functionally, the basal transepidermal waterloss and stratum corneum hydration levels were not different but the barrier recovery rate and integrity tended to be better than those of the non-treated skin. When the epidermis was observed by transmission electron microscopy, the count and secretion of the lamellar body in the stratum corneum-granulosa interface were significantly increased. These results suggest that the newly synthesized Adi-pep can improve lipid synthesis, differentiation, and the recovery of skin barrier function.

Research paper thumbnail of 393 Immune characteristics of scalp and skin psoriasis

Journal of Investigative Dermatology, 2019

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