abu saleh - Academia.edu (original) (raw)
Related Authors
Patuakhali Science and Technology University, Bangladesh
Uploads
Papers by abu saleh
The study was conducted at the nuclear breeder farm of black Bengal goat belongs to the Departmen... more The study was conducted at the nuclear breeder farm of black Bengal goat belongs to the Department of Pathology, Faculty of Veterinary Science and Department of Animal breeding and Genetics, Bangladesh Agricultural University, Mymensingh from March to September, 2008. A total of 10 breeding bucks were used as experimental animal. The aim of the investigation was to analyze of goat semen by using Eosin-Nigrosin stain and Rose Bengal stain under differential interference microscope. The study included investigation of the age in month, ejaculation volume (ml), mass activity of the semen, spermatozoa motility (%), viable % spermatozoa and normal spermatozoa, and morphologically abnormal sperm especially in head that included the acrosome, midpiece, and tail. Semen was collected twice in a week from each buck by using Artificial Vagina method. Age of bucks were 14.9±4.1 months, ejaculate volume of semen was 0.5±0.3 ml, motility was 80.8±3.5 (%), mass activity was 4.The percentage of via...
Journal of Biological Chemistry, 2002
Oncogene, 2004
The type I interferons (IFNs) bind surface receptors, induce JAK kinases and activate STAT transc... more The type I interferons (IFNs) bind surface receptors, induce JAK kinases and activate STAT transcription factors to stimulate the transcription of genes downstream of IFN-stimulated response elements (ISREs). In this study, we demonstrate that IFNaR2, a subunit of the type I IFN receptor, is proteolytically cleaved in a regulated manner. Immunoblotting shows that multi-step cleavage occurs in response to phorbol ester (PMA) and IFN-a, generating both a transmembrane 'stub' and the intracellular domain (ICD), similar to Notch proteolysis. Isolated membrane fractions process IFNaR2 to release the ICD. A chimeric receptor construct is utilized to show that cleavage requires the presenilins and occurs in response to epidermal growth factor and protein kinase C-d overexpression, as well as PMA and type I IFNs. Fluorescence microscopy demonstrates that a green fluorescent protein-ICD fusion localizes predominantly to the nucleus. A fusion between the ICD and the Gal4 DNA-binding domain represses transcription, in a histone deacetylasedependent manner, of a Gal4 upstream activating sequence-regulated reporter, while overexpression of the ICD alone represses transcription of a reporter linked to an ISRE. Proteolytic cleavage events may facilitate receptor turnover or, more likely, function as a mechanism for signaling similar to that employed by Notch and the Alzheimer's precursor protein.
The study was conducted at the nuclear breeder farm of black Bengal goat belongs to the Departmen... more The study was conducted at the nuclear breeder farm of black Bengal goat belongs to the Department of Pathology, Faculty of Veterinary Science and Department of Animal breeding and Genetics, Bangladesh Agricultural University, Mymensingh from March to September, 2008. A total of 10 breeding bucks were used as experimental animal. The aim of the investigation was to analyze of goat semen by using Eosin-Nigrosin stain and Rose Bengal stain under differential interference microscope. The study included investigation of the age in month, ejaculation volume (ml), mass activity of the semen, spermatozoa motility (%), viable % spermatozoa and normal spermatozoa, and morphologically abnormal sperm especially in head that included the acrosome, midpiece, and tail. Semen was collected twice in a week from each buck by using Artificial Vagina method. Age of bucks were 14.9±4.1 months, ejaculate volume of semen was 0.5±0.3 ml, motility was 80.8±3.5 (%), mass activity was 4.The percentage of via...
Journal of Biological Chemistry, 2002
Oncogene, 2004
The type I interferons (IFNs) bind surface receptors, induce JAK kinases and activate STAT transc... more The type I interferons (IFNs) bind surface receptors, induce JAK kinases and activate STAT transcription factors to stimulate the transcription of genes downstream of IFN-stimulated response elements (ISREs). In this study, we demonstrate that IFNaR2, a subunit of the type I IFN receptor, is proteolytically cleaved in a regulated manner. Immunoblotting shows that multi-step cleavage occurs in response to phorbol ester (PMA) and IFN-a, generating both a transmembrane 'stub' and the intracellular domain (ICD), similar to Notch proteolysis. Isolated membrane fractions process IFNaR2 to release the ICD. A chimeric receptor construct is utilized to show that cleavage requires the presenilins and occurs in response to epidermal growth factor and protein kinase C-d overexpression, as well as PMA and type I IFNs. Fluorescence microscopy demonstrates that a green fluorescent protein-ICD fusion localizes predominantly to the nucleus. A fusion between the ICD and the Gal4 DNA-binding domain represses transcription, in a histone deacetylasedependent manner, of a Gal4 upstream activating sequence-regulated reporter, while overexpression of the ICD alone represses transcription of a reporter linked to an ISRE. Proteolytic cleavage events may facilitate receptor turnover or, more likely, function as a mechanism for signaling similar to that employed by Notch and the Alzheimer's precursor protein.