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Papers by ad fas

Digestive Diseases and Sciences, 1997

Active cell death induced by ligation of the Fasantigen (Fas-Ag) with its antibody, Fas ligand (F... more Active cell death induced by ligation of the Fasantigen (Fas-Ag) with its antibody, Fas ligand (Fas-L),has been known to play a major role in cell killing viaapoptosis by cytotoxic T lymphocytes (CTL). Thus, in liver transplantation, Fas-Agexpression of hepatocytes and its modification byimmunosuppressive agents such as FK 506 or CsA cantheoretically influence allograft survival. Mousehepatocytes (BALB/c) were isolated and cultured with orwithout FK 506 or CsA, and Fas-Ag expression wasdetermined by flow cytometry. Fas-Ag expression in thecontrol was 17.2 ± 2.5% after 24 hr of culture.When FK 506 or CsA was added, Fas-Ag expression withFK 506 at a concentration of 0.01-0.1 μg/ml wassignificantly lower than that with CsA (P < 0.05).When the cells were incubated with apoptosis-inducing anti-Fas-Ag monoclonal antibody, agarose gelelectrophoresis of the control cells yielded a typicalpattern of DNA fragmentations. The cells with FK 506 at0.01 μg/ml yielded the least DNA fragmentation. These findings suggested that in the in vivosetting, the hepatocytes of the allograft would have alower chance of being attacked by CTL in the hosttreated with FK 506.

Journal of Surgical Research, 2003

man colon cancer cells were studied. DOX and INDO were used at clinically achievable concentratio... more man colon cancer cells were studied. DOX and INDO were used at clinically achievable concentrations. Beta-catenin levels were determined by western blotting. Beta-catenin-dependent transcription was measured by TOPFlash reporter assay. Cell viability was determined by tiazolyl blue (MTT) reduction and growth was determined by direct cell counting. In vivo, tumor growth was determined by measurement of subcutaneous xenografts in BALB/c nude mice. DOX was administered in drinking water, and INDO by IP injection. Results: In vitro, both DOX and INDO exposure result in a dose dependent down-regulation of beta-catenin protein and beta-catenindependent gene transcription, resulting in inhibition of cell viability and proliferation. A synergistic relationship of combining these two agents is evident. In vivo studies show an enhanced anti-tumor effect of combined DOX and INDO treatment on SW480 xenograft growth ( ). Conclusions: DOX and INDO suppress beta-catenin expression and transcription activity in colon cancer cells, inhibiting cancer cell growth in vitro and in vivo. DOX and INDO may be of clinical use in therapeutic or chemopreventative strategies for colon cancer.

$Research paper thumbnail of Effects of fulvic fractions on the pH-dependent sorption of Cu(II) to kaolinite$

Water Research, 2006

Sorption of copper on kaolinite in the absence and presence of four fulvic acid (FA) fractions fr... more Sorption of copper on kaolinite in the absence and presence of four fulvic acid (FA) fractions fractionated using XAD-8 resin, including F4.8, F7.0, F11.0 and Feth fractions (eluted by pH4.8 buffer, pH7.0 buffer, pH11.0 buffer, and ethanol (95%), respectively, was investigated by batch experiments. Results showed that the binding of Cu(II) by pure kaolinite increased with an increase in pH values. The presence of each FA fraction significantly affects the sorption of Cu(II) to kaolinite. Below pH 6.3, Cu(II) sorption was pronouncedly promoted after adding FA fraction to binary systems, compared to that in pure kaolinite suspensions.

Proceedings of The National Academy of Sciences, 1994

Al momento attuale non è possibile avere un quadro epidemiologico completo del diabete nella popo... more Al momento attuale non è possibile avere un quadro epidemiologico completo del diabete nella popolazione marchigiana, ma sono disponibili i risultati di indagini condotte su problemi specifici e riferiti ad alcune aree della Regione . Fa eccezione il diabete mellito di tipo 1 per cui sono disponibili i dati del Registro di Incidenza attivo dal 1990. Fonti informative: -RIDI (Registro Italiano Diabete mellito Insulino-dipendente); -ISTAT; -Studio sulla Prevalenza del Diabete Mellito tipo II sulla popolazione della ZT 6, Fabriano (1999-2000); -Sorveglianza delle amputazioni non traumatiche degli arti inferiori, regione Marche (1997-1998); -Studio Q.U.A.D.R.I. (Qualità dell'Assistenza alle persone Diabetiche nelle Regioni Italiane), 2004; -Studio sulla mortalità dei soggetti diabetici residenti nella provincia di Ancona (coorte 1988-1993)

Cancer Gene Therapy, 2008

Ad-PPE-Fas-c is an adenovector that expresses Fas-c under the control of the modified pre-proendo... more Ad-PPE-Fas-c is an adenovector that expresses Fas-c under the control of the modified pre-proendothelin-1 (PPE-1) promoter. Fas-c is a chimeric death receptor containing the extracellular portion of tumour necrosis factor 1 receptor (TNFR1) and the transmembrane and intracellular portion of Fas. We recently demonstrated that Ad-PPE-Fas-c induced Fas-receptor-mediated endothelial cell apoptosis. Previously, doxorubicin was shown to enhance Fas-receptor clustering and the induction of its cascade. Therefore, the goal of this work was to test whether doxorubicin augments the capacity of Ad-PPE-Fas-c to induce endothelial cell apoptosis and to elucidate whether either the death-receptor-mediated apoptotic cascade or the mitochondria-associated apoptotic cascade is involved in the combined treatment effect. We found that a combined treatment of Ad-PPE-Fas-c and doxorubicin synergistically induced a reduction in endothelial cell viability and apoptosis. z-IETD-FMK, a caspase-8 inhibitor, and z-LEHD-FMK, a caspase-9 inhibitor, significantly decreased apoptosis induced by the combined treatment. Systemically administered combined therapy significantly reduced the lung metastases burden (70%) in mice as compared to each treatment alone. Thus, a combined treatment of Ad-PPE-Fas-c gene therapy and chemotherapy may be effective in the treatment of metastatic diseases and both the Fas cascade and the mitochondria-associated cascade are essential for this effect.

Food and Chemical Toxicology, 1999

AbstractÐFumonisins and fusaric acid (FA) are mycotoxins produced by Fusarium moniliforme and oth... more AbstractÐFumonisins and fusaric acid (FA) are mycotoxins produced by Fusarium moniliforme and other Fusarium which grow on corn. Fumonisins cause animal toxicities associated with F. moniliforme and, like F. moniliforme, they are suspected human oesophageal carcinogens. Toxic synergism was obtained by simultaneous administration of FA and fumonisin B 1 to chicks in ovo. To determine the eect of FA on in vivo toxicity of F. moniliforme culture material (CM), male rats (12 groups, n = 5/ group) were fed diets containing 0.025, 0.10 or 2.5% CM (providing dietary levels of 3.4, 18.4 or 437 ppm fumonisins, respectively) to which, at each CM level, 0, 20, 100 or 400 ppm FA were added. Additionally, an FA control group was fed 400 ppm FA only and an untreated control group was given neither FA nor culture material. Apoptosis and other eects consistent with those caused by fumonisins were present in the kidneys of animals fed 0.025% or more CM and in the livers of animals fed 2.5% CM. FA was without eect. No dierences between the untreated and FA control groups were noted and no dierences among the four groups (0±400 ppm FA) fed 0.025% CM, the four groups fed 0.10% CM or the four groups fed 2.5% CM were apparent. Thus, FA exerted no synergistic, additive or antagonistic eects on the subchronic in vivo toxicity of fumonisin-producing F. moniliforme. #