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Papers by ali karami

International Journal of Control

Open access Macedonian journal of medical sciences, Jan 15, 2017

Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases... more Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases is lung transplantation. Limitation and shortage of donor organs cause to appear tissue engineering field. Decellularization is a hope for producing intact ECM in the development of engineered organs. The goal of the decellularization process is to remove cellular and nuclear material while retaining lung three-dimensional and molecular proteins. Different concentration of detergents was used for finding the best approach in lung decellularization. In this study, three-time approaches (24, 48 and 96 h) with four detergents (CHAPS, SDS, SDC and Triton X-100) were used for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein composition which have significant roles in differentiation and migration of stem cells. This comparative study determined that variable decellularization approaches can cause significantly different effects on decellularize...

Journal of Electrical Engineering and Technology, 2008

This paper presents a method for estimating the transient stability status of the power system us... more This paper presents a method for estimating the transient stability status of the power system using radial basis function (REF) neural network with a fast hybrid training approach. A normalized transient energy margin (ΔV n ) has been obtained by the potential energy boundary ...

ABSTRACT This article may be used for research, teaching, and private study purposes. Any substan... more ABSTRACT This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Http Dx Doi Org 10 1080 00986445 2012 715105, Dec 21, 2012

ABSTRACT This article may be used for research, teaching, and private study purposes. Any substan... more ABSTRACT This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Detection and identification of microbial agents is important step for accurate diagnosis of infe... more Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods like culture, subculture, biochemical, immunological or other typing process are very time consuming, labor intensive, expensive and results are presented very late for the physician or the patient. There have been tremendous efforts to develop new rapid detection methods for microbial agents specially by indirectly analyzing antibodies to the agents or directly detection specific antigens by serological methods. But with advances in Molecular understanding of microbial molecules specially DNA, RNA and proteins and more importantly having rapid genome sequence of infectious agents it hase been great progress in developing new fast detection and identification methods. Molecular Methods like PCR and its derivatives like nested PCR, Real time PCR, RFLP, AFLP, next generation sequencing and development...

One of the major goals of the bio defense programs of every nation is to prevent from threats of ... more One of the major goals of the bio defense programs of every nation is to prevent from threats of Biological agents. The main questions are: which agent? by whom? where? and how it will be delivered? it is very difficult to answer these questions. Various numbers of biological agents, simplicity of agent development, mass production ad delivery and major issue is if agent is natural induced or deliberately released other problems like early warning, rapid detection and identification due incubation periods of most infectious agents. in this regards proactive bio defense programs will be discussed to prevent from development of biological threats. Focus of proactive bio defense program is to prevent the bio threat actors from access to biological agents, production or capacity to release it. in proactive biodefense program we have to work together in the local, regional and international level of to prevent terrorist from access to the agents and capability to develop biological weapo...

Enhancing public health is the major goal of all countries for public welfare. Passive defense is... more Enhancing public health is the major goal of all countries for public welfare. Passive defense is peaceful strategy to tackle emerging and reemerging threats against public safety and security. In this study we have conducted research plan to compare public health strategy of various nations from developed to under development countries on the base of passive defense criteria. With more than 20 years of experiences on research, teaching and education around the world on managing health care crisis, I have identified new component require to add to the public health strategy planning and education system in our region with special threats not available to developed nations. Iran and Middle east region is in forefront of several severe public health treats from war to terrorist activities to regional instability. I have proposed to use passive defense, proactive defense and civil protection criteria on health issues to be prepared against these kind of threats. In this presentation I ...

Point Of Care (POC) Diagnostics from Bench to Market Ali Karami Research Centre of Molecular Biol... more Point Of Care (POC) Diagnostics from Bench to Market Ali Karami Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences. Karami@bmsu.ac.ir Point-of-care test (POC) also known as bedside, near-patient testing is defined as medical testing at or near the site of patient care.The current diagnostic laboratory system has been slow to change, and when properly evaluated is in need of change to a more patient-centered system (personalized medicine and companion diagnostics). The market for in vitro diagnostic (IVD) testing is estimated to be 54billionin2013,withPOC,accordingtoseveralmarketresearchcompanyreports,representing1454 billion in 2013, with POC, according to several market research company reports, representing 14% to 15% of the market. With the evolution of blood glucose, blood gas, urinalysis, pregnancy, and coagulation testing in the mid-1990s, the menu of POCs has expanded from fewer than 10 tests available in 1995 to approximately 110 tests available today. It is predicted that point of care testing market will be approximately US54billionin2013,withPOC,accordingtoseveralmarketresearchcompanyreports,representing14...

triphosphates (dNTPs) to generate double-stranded products [Figure 1]. By raising and lowering th... more triphosphates (dNTPs) to generate double-stranded products [Figure 1]. By raising and lowering the temperature of the reaction mixture, the two strands of the DNA product are separated and can serve as templates for the next round of annealing and extension, and the process is repeated. [2] Although PCR has been widely used by researchers, it requires thermocycling to separate the two DNA strands. This characteristic has limited its application in the field. On the other hand, several isothermal target amplification methods have been developed in the two past decades without using a thermocycler machine. These non-PCR-based techniques have been developed according to new findings in molecular biology of DNA/RNA synthesis and some accessory proteins and their mimicking in vitro for nucleic acid amplification. We describe here the best-known isothermal amplification methods (such as transcriptionmediated amplification [TMA] or self-sustained sequence replication [3SR], nucleic acid sequence-based amplification [NASBA], signal-mediated amplification of RNA Polymerase chain reaction is the most widely used method for DNA amplification for the detection and identification of infectious diseases, genetic disorders and for other research purposes. However, it requires a thermocycling machine to separate the two DNA strands and then amplify the required fragment. Novel developments in molecular biology of DNA synthesis in vivo demonstrate the possibility of amplifying DNA in isothermal temperature without the need of a thermocycling apparatus. DNA polymerase replicates DNA with various accessory proteins. Therefore, with identification of these proteins, we are able to develop new in vitro isothermal DNA amplification methods by mimicking these in vivo mechanisms. There are several isothermal nucleic acid amplifications, such as transcription-mediated amplification or self-sustained sequence replication, nucleic acid sequence-based amplification, signal-mediated amplification of RNA technology, strand displacement amplification, rolling circle amplification, loop-mediated isothermal amplification of DNA, isothermal multiple displacement amplification, helicase-dependent amplification, single-primer isothermal amplification and circular helicase-dependent amplification. In this paper, we reviewed the nucleic acid amplification methods and their applications in molecular biology. We reviewed the best-known isothermal techniques for DNA/RNA amplification. The above information can be used for the application of valuable molecular diagnostic techniques for the detection of infectious agents in small-scale hospital laboratories in the field. With regards to the properties of isothermal DNA/RNA amplification techniques, the design and development of novel rapid molecular tests for application in field may be possible.

International Journal of Control

Open access Macedonian journal of medical sciences, Jan 15, 2017

Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases... more Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases is lung transplantation. Limitation and shortage of donor organs cause to appear tissue engineering field. Decellularization is a hope for producing intact ECM in the development of engineered organs. The goal of the decellularization process is to remove cellular and nuclear material while retaining lung three-dimensional and molecular proteins. Different concentration of detergents was used for finding the best approach in lung decellularization. In this study, three-time approaches (24, 48 and 96 h) with four detergents (CHAPS, SDS, SDC and Triton X-100) were used for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein composition which have significant roles in differentiation and migration of stem cells. This comparative study determined that variable decellularization approaches can cause significantly different effects on decellularize...

Journal of Electrical Engineering and Technology, 2008

This paper presents a method for estimating the transient stability status of the power system us... more This paper presents a method for estimating the transient stability status of the power system using radial basis function (REF) neural network with a fast hybrid training approach. A normalized transient energy margin (ΔV n ) has been obtained by the potential energy boundary ...

ABSTRACT This article may be used for research, teaching, and private study purposes. Any substan... more ABSTRACT This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Http Dx Doi Org 10 1080 00986445 2012 715105, Dec 21, 2012

ABSTRACT This article may be used for research, teaching, and private study purposes. Any substan... more ABSTRACT This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Detection and identification of microbial agents is important step for accurate diagnosis of infe... more Detection and identification of microbial agents is important step for accurate diagnosis of infectious diseases for treatment or prevention from spread to the society. Classical methods like culture, subculture, biochemical, immunological or other typing process are very time consuming, labor intensive, expensive and results are presented very late for the physician or the patient. There have been tremendous efforts to develop new rapid detection methods for microbial agents specially by indirectly analyzing antibodies to the agents or directly detection specific antigens by serological methods. But with advances in Molecular understanding of microbial molecules specially DNA, RNA and proteins and more importantly having rapid genome sequence of infectious agents it hase been great progress in developing new fast detection and identification methods. Molecular Methods like PCR and its derivatives like nested PCR, Real time PCR, RFLP, AFLP, next generation sequencing and development...

One of the major goals of the bio defense programs of every nation is to prevent from threats of ... more One of the major goals of the bio defense programs of every nation is to prevent from threats of Biological agents. The main questions are: which agent? by whom? where? and how it will be delivered? it is very difficult to answer these questions. Various numbers of biological agents, simplicity of agent development, mass production ad delivery and major issue is if agent is natural induced or deliberately released other problems like early warning, rapid detection and identification due incubation periods of most infectious agents. in this regards proactive bio defense programs will be discussed to prevent from development of biological threats. Focus of proactive bio defense program is to prevent the bio threat actors from access to biological agents, production or capacity to release it. in proactive biodefense program we have to work together in the local, regional and international level of to prevent terrorist from access to the agents and capability to develop biological weapo...

Enhancing public health is the major goal of all countries for public welfare. Passive defense is... more Enhancing public health is the major goal of all countries for public welfare. Passive defense is peaceful strategy to tackle emerging and reemerging threats against public safety and security. In this study we have conducted research plan to compare public health strategy of various nations from developed to under development countries on the base of passive defense criteria. With more than 20 years of experiences on research, teaching and education around the world on managing health care crisis, I have identified new component require to add to the public health strategy planning and education system in our region with special threats not available to developed nations. Iran and Middle east region is in forefront of several severe public health treats from war to terrorist activities to regional instability. I have proposed to use passive defense, proactive defense and civil protection criteria on health issues to be prepared against these kind of threats. In this presentation I ...

Point Of Care (POC) Diagnostics from Bench to Market Ali Karami Research Centre of Molecular Biol... more Point Of Care (POC) Diagnostics from Bench to Market Ali Karami Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences. Karami@bmsu.ac.ir Point-of-care test (POC) also known as bedside, near-patient testing is defined as medical testing at or near the site of patient care.The current diagnostic laboratory system has been slow to change, and when properly evaluated is in need of change to a more patient-centered system (personalized medicine and companion diagnostics). The market for in vitro diagnostic (IVD) testing is estimated to be 54billionin2013,withPOC,accordingtoseveralmarketresearchcompanyreports,representing1454 billion in 2013, with POC, according to several market research company reports, representing 14% to 15% of the market. With the evolution of blood glucose, blood gas, urinalysis, pregnancy, and coagulation testing in the mid-1990s, the menu of POCs has expanded from fewer than 10 tests available in 1995 to approximately 110 tests available today. It is predicted that point of care testing market will be approximately US54billionin2013,withPOC,accordingtoseveralmarketresearchcompanyreports,representing14...

triphosphates (dNTPs) to generate double-stranded products [Figure 1]. By raising and lowering th... more triphosphates (dNTPs) to generate double-stranded products [Figure 1]. By raising and lowering the temperature of the reaction mixture, the two strands of the DNA product are separated and can serve as templates for the next round of annealing and extension, and the process is repeated. [2] Although PCR has been widely used by researchers, it requires thermocycling to separate the two DNA strands. This characteristic has limited its application in the field. On the other hand, several isothermal target amplification methods have been developed in the two past decades without using a thermocycler machine. These non-PCR-based techniques have been developed according to new findings in molecular biology of DNA/RNA synthesis and some accessory proteins and their mimicking in vitro for nucleic acid amplification. We describe here the best-known isothermal amplification methods (such as transcriptionmediated amplification [TMA] or self-sustained sequence replication [3SR], nucleic acid sequence-based amplification [NASBA], signal-mediated amplification of RNA Polymerase chain reaction is the most widely used method for DNA amplification for the detection and identification of infectious diseases, genetic disorders and for other research purposes. However, it requires a thermocycling machine to separate the two DNA strands and then amplify the required fragment. Novel developments in molecular biology of DNA synthesis in vivo demonstrate the possibility of amplifying DNA in isothermal temperature without the need of a thermocycling apparatus. DNA polymerase replicates DNA with various accessory proteins. Therefore, with identification of these proteins, we are able to develop new in vitro isothermal DNA amplification methods by mimicking these in vivo mechanisms. There are several isothermal nucleic acid amplifications, such as transcription-mediated amplification or self-sustained sequence replication, nucleic acid sequence-based amplification, signal-mediated amplification of RNA technology, strand displacement amplification, rolling circle amplification, loop-mediated isothermal amplification of DNA, isothermal multiple displacement amplification, helicase-dependent amplification, single-primer isothermal amplification and circular helicase-dependent amplification. In this paper, we reviewed the nucleic acid amplification methods and their applications in molecular biology. We reviewed the best-known isothermal techniques for DNA/RNA amplification. The above information can be used for the application of valuable molecular diagnostic techniques for the detection of infectious agents in small-scale hospital laboratories in the field. With regards to the properties of isothermal DNA/RNA amplification techniques, the design and development of novel rapid molecular tests for application in field may be possible.