alireza javadi - Academia.edu (original) (raw)

Papers by alireza javadi

Research paper thumbnail of Extracellular vesicles from serum samples of mycobacteria patients induced cell death of THP-1 monocyte and PBMC

BMC Pulmonary Medicine, 2022

Background Extracellular vesicles (EVs) play a key role in cell communication and the pathogenesi... more Background Extracellular vesicles (EVs) play a key role in cell communication and the pathogenesis of some diseases. EVs may accelerate cell death during the course of mycobacterial infection and are also considered as a new vaccine design, drug delivery, and biomarker candidates. The current study evaluates the effects of EVs from serum samples of mycobacteria-infected patients on THP-1 monocytes and PBMC cells. Method EVs were purified from the serum, then cultured separately with THP-1 monocytes and PBMCs. The cell death was determined through annexin V-FITC and PI staining. GW4869, an EVs inhibitor, was used to determine if EVs released from serum could increase THP-1 monocytes cell death. Results The cell death was significantly increased in the presence of 10 µg/ml and 5 µg/ml concentrations of the purified EVs (p

Research paper thumbnail of Sarcoidosis or Tuberculosis? Detecting Mycobacterium tuberculosis Complex DNA in Sarcoidosis Granulomas

Current Respiratory Medicine Reviews, 2018

Background: Sarcoidosis is a granulomatous inflammatory disorder with unknown etiology and its re... more Background: Sarcoidosis is a granulomatous inflammatory disorder with unknown etiology and its relation with Mycobacterium tuberculosis (M. tuberculosis) has been debated for years. In this study, we have investigated the presence of mycobacterial DNA in sarcoidosis tissue samples. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) tissues of 33 patients with sarcoidosis were analyzed for the presence of mycobacterial DNA. Genomic DNA extraction was done by QIAamp DNA FFPE Tissue Kit. Polymerase chain reaction using insertion element IS6110 of M. tuberculosis complex (MTC) was applied by commercial kits (GeneProof) for all individuals. The results were compared with 27 patients with tuberculosis and 5 other patients associated with granulomatous disease of the lung. All cases had confirmed granulomatous inflammation in their histopathological examination. Results: In this study, the IS6110 repetitive DNA element of (MTC) was not detected in any of the tissue samples from the patients with sarcoidosis. Of the 33 sarcoidosis patients, 30 (90.1%) had negative results for IS6110 and despite the repeated attempts of DNA extraction for three patients (9.1%), strong inhibitor made constant negative outcomes. In contrast, in patients with tuberculosis, 22 (81.5%) had positive results, three had (11.1%) negative results and 2 patients (7.4%) showed negative results with strong inhibitor. IS6110 was not found in any of the control group patients. Discussion: This study does not support the presence of M. tuberculosis in tissues of patients with sarcoidosis as a microbial pathogen or trigger of the immune response. Due to difficulties in diagnosis of sarcoidosis and different methods for diagnosis of M. tuberculosis, the impact of M. tuberculosis as a possible aetiological agent in sarcoidosis has been the point of debate.

Research paper thumbnail of Insertion/Deletion Polymorphisms and Serum Angiotensin-converting Enzyme Levels in Iranian Patients with Sarcoidosis

Iranian journal of public health, 2016

Sarcoidosis is a multisystem inflammatory disease of unknown origin with characterization of smal... more Sarcoidosis is a multisystem inflammatory disease of unknown origin with characterization of small granulomas. Angiotensin-converting enzyme (ACE) is a pathophysiologic marker of sarcoidosis. We present the ACE insertion/deletion (I/D) polymorphism in correlation with serum ACE level in Iranian patients with sarcoidosis. From Jan 2014 to Jan 2015, 102 Iranian patients who histopathologically diagnosed for sarcoidosis and 192 healthy age and sex-matched controls were recruited. PCR was used for detection of I/D polymorphism in ACE gene. Frequency of II/ID/DD genotype in sarcoidosis disease was 17%, 35.5%, and 47.1%, respectively. The frequency of D allele was 0.65. A significant association between I/D genotypes and mean of sACE level was seen (DD=85.2±22.9, P<0.001). More frequent genotype in sarcoidosis patients was DD (47%), ID genotype (45.9%) was found more in controls. Logistic regression analysis adjusting age and sex showed that ID to II (OR=0.35, 95%CI=0.17-0.73, P=0.005)...

Research paper thumbnail of The first designed PCR-RFLP technique for butyrophilin-like 2 rs2076530 polymorphism in sarcoidosis

European Respiratory Journal, 2016

Background: Sarcoidosis is a multifactorial immune disorder with uncertain origin. A single nucle... more Background: Sarcoidosis is a multifactorial immune disorder with uncertain origin. A single nucleotide polymorphism (G→A, rs2076530) in the butyrophilin-like 2 (BTNL2) gene results in formation of truncating protein. This study aimed to genotype predisposition of the BTNL2 rs2076530 polymorphism in Iranian patients with sarcoidosis. Materials and Methods: In this study, 80 patients with sarcoidosis and 80 healthy individuals were included. The rs2076530 polymorphism of BTNL2 gene was genotyped using the first designed PCR-RFLP method by AvrII restriction enzyme and confirmed by DNA sequencing (Capillary electrophoresis 3130, ABI). Results: A statistical significant was observed in proportion of patient9s AA (67.9%) in comparison to AA (40.7%) (OR=3.07,95%CI:1.40-6.7, P=0.004) in controls. Also, the significant difference was statistically found between A (62.5%) in subjects and A (43.8%) allele in controls (OR=2.14,95%CI:1.37-3.35, P=0.001). However, no statistical difference was seen between case and controls based on AA and AG proportion. Conclusion: Our data emphasized the association of BTNL2 rs2076530 A allele with sarcoidosis susceptibility in Iranian patients. The first designed PCR-RFLP method for rs2076530 A allele can be applicable in resource-limited countries.

Research paper thumbnail of Waterpipe tobacco smoking may potentiate risk of fungal and bacterial infections

European Respiratory Journal, 2016

Background: Hookah, as a popular practice among adults, was first introduced by the Asian countri... more Background: Hookah, as a popular practice among adults, was first introduced by the Asian countries; however, it is now increasing worldwide. Although several clinical or experimental studies on waterpipe (WP) tobacco smoking highlights that this addictive behavior causes some devastating diseases such as lung cancer and respiratory diseases, further investigations on health effects of a WP tobacco smoking are still required as a serious attention. In the current study, we aimed to investigate the possible infectious agents in Iranian hookahs. Materials and Methods: Fifty water samples were taken from the hoses and water jars of the hookahs and cultured on Blood agar, MacConkey agar and Sabouraud agar mediums. Mycobacterial culture has been done on Lowenstein–Jensen medium and direct smear examination for mycobacterium were performed for all of the fifty samples. Results: The findings of this study show that 4 gram-negative bacterial species including Pseudomonas aeruginosa , Klebsiella spp , Escherichia coli , and Enterobacter and 3 gram-positive bacteria strains including Choagulase Neg. Staph , Strep viridians and Micrococcus were detected. Yeast-fungi like Candida , and Mycelial-fungi including Fusarium and Aspergillous Sp were also determined by fungal cultures on Sabouraud agar plates. The results of mycobacterial microscopic examinations and culture were all negative. Conclusions: Our results indicated to infectious potential in water of WP. Thus, simply believing of people for safety of WP than cigarettes could be considered as a wrong idea and should be taking care in human health. Keywords: waterpipe tobacco smoking, conventional TB culture, conventional fungal and microbiological culture.

Research paper thumbnail of Extracellular vesicles from serum samples of mycobacteria patients induced cell death of THP-1 monocyte and PBMC

BMC Pulmonary Medicine, 2022

Background Extracellular vesicles (EVs) play a key role in cell communication and the pathogenesi... more Background Extracellular vesicles (EVs) play a key role in cell communication and the pathogenesis of some diseases. EVs may accelerate cell death during the course of mycobacterial infection and are also considered as a new vaccine design, drug delivery, and biomarker candidates. The current study evaluates the effects of EVs from serum samples of mycobacteria-infected patients on THP-1 monocytes and PBMC cells. Method EVs were purified from the serum, then cultured separately with THP-1 monocytes and PBMCs. The cell death was determined through annexin V-FITC and PI staining. GW4869, an EVs inhibitor, was used to determine if EVs released from serum could increase THP-1 monocytes cell death. Results The cell death was significantly increased in the presence of 10 µg/ml and 5 µg/ml concentrations of the purified EVs (p

Research paper thumbnail of Sarcoidosis or Tuberculosis? Detecting Mycobacterium tuberculosis Complex DNA in Sarcoidosis Granulomas

Current Respiratory Medicine Reviews, 2018

Background: Sarcoidosis is a granulomatous inflammatory disorder with unknown etiology and its re... more Background: Sarcoidosis is a granulomatous inflammatory disorder with unknown etiology and its relation with Mycobacterium tuberculosis (M. tuberculosis) has been debated for years. In this study, we have investigated the presence of mycobacterial DNA in sarcoidosis tissue samples. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) tissues of 33 patients with sarcoidosis were analyzed for the presence of mycobacterial DNA. Genomic DNA extraction was done by QIAamp DNA FFPE Tissue Kit. Polymerase chain reaction using insertion element IS6110 of M. tuberculosis complex (MTC) was applied by commercial kits (GeneProof) for all individuals. The results were compared with 27 patients with tuberculosis and 5 other patients associated with granulomatous disease of the lung. All cases had confirmed granulomatous inflammation in their histopathological examination. Results: In this study, the IS6110 repetitive DNA element of (MTC) was not detected in any of the tissue samples from the patients with sarcoidosis. Of the 33 sarcoidosis patients, 30 (90.1%) had negative results for IS6110 and despite the repeated attempts of DNA extraction for three patients (9.1%), strong inhibitor made constant negative outcomes. In contrast, in patients with tuberculosis, 22 (81.5%) had positive results, three had (11.1%) negative results and 2 patients (7.4%) showed negative results with strong inhibitor. IS6110 was not found in any of the control group patients. Discussion: This study does not support the presence of M. tuberculosis in tissues of patients with sarcoidosis as a microbial pathogen or trigger of the immune response. Due to difficulties in diagnosis of sarcoidosis and different methods for diagnosis of M. tuberculosis, the impact of M. tuberculosis as a possible aetiological agent in sarcoidosis has been the point of debate.

Research paper thumbnail of Insertion/Deletion Polymorphisms and Serum Angiotensin-converting Enzyme Levels in Iranian Patients with Sarcoidosis

Iranian journal of public health, 2016

Sarcoidosis is a multisystem inflammatory disease of unknown origin with characterization of smal... more Sarcoidosis is a multisystem inflammatory disease of unknown origin with characterization of small granulomas. Angiotensin-converting enzyme (ACE) is a pathophysiologic marker of sarcoidosis. We present the ACE insertion/deletion (I/D) polymorphism in correlation with serum ACE level in Iranian patients with sarcoidosis. From Jan 2014 to Jan 2015, 102 Iranian patients who histopathologically diagnosed for sarcoidosis and 192 healthy age and sex-matched controls were recruited. PCR was used for detection of I/D polymorphism in ACE gene. Frequency of II/ID/DD genotype in sarcoidosis disease was 17%, 35.5%, and 47.1%, respectively. The frequency of D allele was 0.65. A significant association between I/D genotypes and mean of sACE level was seen (DD=85.2±22.9, P<0.001). More frequent genotype in sarcoidosis patients was DD (47%), ID genotype (45.9%) was found more in controls. Logistic regression analysis adjusting age and sex showed that ID to II (OR=0.35, 95%CI=0.17-0.73, P=0.005)...

Research paper thumbnail of The first designed PCR-RFLP technique for butyrophilin-like 2 rs2076530 polymorphism in sarcoidosis

European Respiratory Journal, 2016

Background: Sarcoidosis is a multifactorial immune disorder with uncertain origin. A single nucle... more Background: Sarcoidosis is a multifactorial immune disorder with uncertain origin. A single nucleotide polymorphism (G→A, rs2076530) in the butyrophilin-like 2 (BTNL2) gene results in formation of truncating protein. This study aimed to genotype predisposition of the BTNL2 rs2076530 polymorphism in Iranian patients with sarcoidosis. Materials and Methods: In this study, 80 patients with sarcoidosis and 80 healthy individuals were included. The rs2076530 polymorphism of BTNL2 gene was genotyped using the first designed PCR-RFLP method by AvrII restriction enzyme and confirmed by DNA sequencing (Capillary electrophoresis 3130, ABI). Results: A statistical significant was observed in proportion of patient9s AA (67.9%) in comparison to AA (40.7%) (OR=3.07,95%CI:1.40-6.7, P=0.004) in controls. Also, the significant difference was statistically found between A (62.5%) in subjects and A (43.8%) allele in controls (OR=2.14,95%CI:1.37-3.35, P=0.001). However, no statistical difference was seen between case and controls based on AA and AG proportion. Conclusion: Our data emphasized the association of BTNL2 rs2076530 A allele with sarcoidosis susceptibility in Iranian patients. The first designed PCR-RFLP method for rs2076530 A allele can be applicable in resource-limited countries.

Research paper thumbnail of Waterpipe tobacco smoking may potentiate risk of fungal and bacterial infections

European Respiratory Journal, 2016

Background: Hookah, as a popular practice among adults, was first introduced by the Asian countri... more Background: Hookah, as a popular practice among adults, was first introduced by the Asian countries; however, it is now increasing worldwide. Although several clinical or experimental studies on waterpipe (WP) tobacco smoking highlights that this addictive behavior causes some devastating diseases such as lung cancer and respiratory diseases, further investigations on health effects of a WP tobacco smoking are still required as a serious attention. In the current study, we aimed to investigate the possible infectious agents in Iranian hookahs. Materials and Methods: Fifty water samples were taken from the hoses and water jars of the hookahs and cultured on Blood agar, MacConkey agar and Sabouraud agar mediums. Mycobacterial culture has been done on Lowenstein–Jensen medium and direct smear examination for mycobacterium were performed for all of the fifty samples. Results: The findings of this study show that 4 gram-negative bacterial species including Pseudomonas aeruginosa , Klebsiella spp , Escherichia coli , and Enterobacter and 3 gram-positive bacteria strains including Choagulase Neg. Staph , Strep viridians and Micrococcus were detected. Yeast-fungi like Candida , and Mycelial-fungi including Fusarium and Aspergillous Sp were also determined by fungal cultures on Sabouraud agar plates. The results of mycobacterial microscopic examinations and culture were all negative. Conclusions: Our results indicated to infectious potential in water of WP. Thus, simply believing of people for safety of WP than cigarettes could be considered as a wrong idea and should be taking care in human health. Keywords: waterpipe tobacco smoking, conventional TB culture, conventional fungal and microbiological culture.