amira ahmed abdelsallam mohamed - Academia.edu (original) (raw)
Papers by amira ahmed abdelsallam mohamed
Assiut University Journal of Multidisciplinary Scientific Research
The Journal of Infection in Developing Countries, 2015
Introduction: Shigella flexneri is a Gram-negative bacteria that has the ability to invade the ep... more Introduction: Shigella flexneri is a Gram-negative bacteria that has the ability to invade the epithelium of the colon and cause colon ulcers. Methodology: The ability of isolated Shigella flexneri from bloody diarrhea to cause colon ulcers was investigated by histopathological examination via oral administration of the bacteria to adult male albino Sprague-Dawley rats. The antibacterial activity of thyme oil, ciprofloxacin, and their combination were evaluated in vitro and in vivo. Results: Oral administration of 12 × 108 CFU/mL of S. flexneri was able to cause colon ulcers. Thyme oil had the highest antibacterial activity among other investigated oils (minimum inhibitory concentration [MIC] 150 µL/L). Ciprofloxacin had the highest antimicrobial activity against S. flexneri (MIC 0.4 mg/L). The synergism between thyme oil and ciprofloxacin showed the maximum growth inhibition of S. flexneri. The synergistic activity of thyme oil and ciprofloxacin succeeded in healing the epithelial ...
Journal of the Egyptian Society of Parasitology, 2007
The reaction of the haemolymph and the tissue of infected intermediate hosts, Biomphalaria alexan... more The reaction of the haemolymph and the tissue of infected intermediate hosts, Biomphalaria alexandrina and Bulinus truncatus to Schistosoma mansoni and S. haematobium antigens were investigated using the indirect immunoperoxidase technique. A new technique, Agarose cell block was used in collection of haemolymph which helped in collecting plenty of well formed cells in comparison to the ordinary one using the cytospin. Collected haemolymph and prepared tissues of uninfected and infected B. alexandria and B. truncatus were fixed and then reacted with anti-S. mansoni and anti-S. haematobium IgG polyclonal antibodies. The haemolymph and tissue of infected B. alexandrina and B. truncatus gave a positive peroxidase reaction represented by a brown colour. In haemolymph, the positive peroxidase reaction was detected mainly in the cytoplasm of the amoebocytes. In the tissue, it was detected in epithelial cells lining the tubules, male cells in the lumen of the tubules and in female oogonia ...
MedGenMed : Medscape general medicine, Jan 23, 2007
The activity of a novel enaminone derivative of 4-hydroxyquinoline, BDHQ, was screened for its ef... more The activity of a novel enaminone derivative of 4-hydroxyquinoline, BDHQ, was screened for its effectiveness against murine schistosomiasis by electron microscopy and parasitologic studies. The correlation of these studies with serum levels of IFN-gamma and IgE is described. Two groups of 10 mice each were treated with different doses of BDHQ, and their results were correlated with the control and praziquantel (PZQ)-treated groups. Parasitologic study revealed significant reduction in mature worms and tissue egg loads in BDHQ- and PZQ-treated groups, whereas immature worms revealed significant reduction in BDHQ groups only. The group treated with a higher dose of BDHQ showed significant reductions in intestinal ova count when compared with the PZQ-treated group. Ultrastructural examination of the worm revealed significant degeneration of the spines and tegument in all treated groups, while the genital system was affected in BDHQ-treated groups only. BDHQ showed considerable effect o...
Journal of the Egyptian Society of Parasitology, 2006
The effect of cyclooxygenase-2 (COX-2) inhibitor, such (as meloxicam, and pyocyanin pigment of Ps... more The effect of cyclooxygenase-2 (COX-2) inhibitor, such (as meloxicam, and pyocyanin pigment of Pseudomonas aeruginosa) with and without praziquantel (PZQ) on worms, ova count, bone marrow and blood cells in 7 groups of Schistosoma mansoni infected mice was studied. The results revealed significant decrease of worm burden and ova count in all treated groups as compared to the infected untreated group, while those with combined treatment of PZQ and meloxicam or pyocyanin showed complete eradication of the worm with the highest reduction in the tissue egg load. EM showed extensive swelling and vesiculation of the tegument, completely implanted spines that overlie degenerated muscle layer were obvious in groups treated with either meloxicam or pyocyanin. Hematological study revealed significant increase (P<0.05) of total leucocytic count of PZQ treated group while that treated with either meloxicam or pyocyanin showed significant decrease (P<0.05), but in combination of PZQ with m...
Journal of the Egyptian Society of Parasitology, 2014
The Scientific World Journal, 2012
The present experiment was conducted to study the effect of ethanolic extract of Egyptian propoli... more The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivatedPasteurella multocidavaccine on rabbits challenged with a virulent strain ofPasteurella multocida. Fifty-six New-Zealand rabbits, 6–8 weeks old and non-vaccinated against pasteurellosis, were randomly divided into eight equal groups. The first group was kept as a control for the experiment. The other groups received different treatments with propolis extract, inactivated vaccine, or both. The experiment continued for seven weeks during which clinical signs, body weight, and mortality rate were monitored, and blood samples were collected weekly for evaluating the leukogram, serum biochemistry, and immune response in all groups of animals. At the end of the seventh week, the animals were subjected to challenge with a virulent strain ofPasteurella multocida. Two weeks later, tissue specimens were collected from different organs for histopathol...
International Journal of Stem Cells, 2014
BioMed Research International, 2013
The present study was conducted to study the protective effect of ethanolic extract of propolis g... more The present study was conducted to study the protective effect of ethanolic extract of propolis given subcutaneously (S/C) either alone or in combination with inactivated formalizedPasteurella multocida(P. multocida) vaccine in rabbits challenged with virulentP. multocidastrain. Twenty-eight New-Zealand rabbits, 6–8 weeks old and not vaccinated against pasteurellosis, were randomly divided into four equal groups. Group (1) was kept as nonvaccinated control. Group (2) was injected S/C with propolis. Group (3) was vaccinated (S/C) withP. multocidavaccine only. Group (4) was injected with vaccine mixed with propolis as adjuvant. Groups (2, 3, and 4) received the same doses of propolis and vaccine after 4 weeks as a booster dose. The experiment continued for six weeks during which clinical signs, body weight, and mortality rate were recorded. Blood samples were collected every 2 weeks of treatment for evaluating the erythrogram and biochemical parameters. At the end of six weeks, all gr...
Journal of Men's Health, 2009
journal of men&#x27;s health, Volume 6, Issue 3, Pages 230, September 2009, Authors:Amira... more journal of men&#x27;s health, Volume 6, Issue 3, Pages 230, September 2009, Authors:Amira Helmy Mohamed; Olfat Ali Hammam; Tarek Ramzy El Lithy; Mohamed Mohi El Din Wishahi.
Infection, Genetics and Evolution, 2007
Hepatitis E virus (HEV) is an important cause of hepatitis among young Egyptian adults with high ... more Hepatitis E virus (HEV) is an important cause of hepatitis among young Egyptian adults with high seroprevalence rates seen in both rural areas of the Nile Delta and in suburban Cairo. Because natural antibodies to HEV have been detected in animals and zoonotic transmission is postulated, we surveyed work horses in Cairo for evidence of HEV exposure and viremia. Sera from 200 Cairo work horses were tested by ELISA for the presence of IgG anti-HEV antibody revealed a seropositivity of 13%. Among 100 samples processed for detection of viral genome by means of nested polymerase chain reaction (N-PCR), 4% were positive and indicative of viremia. Viremic animals were less than 1 year old. Relative to PCR-negative horses, PCR-positive animals demonstrated significant elevation of AST (p=0.03). Phylogenetic analysis of a 253-bp fragment, in the ORF-1,2,3 overlap region of the HEV genome from the viremic animals showed that three of these viral strains to be identical, and closely related (97-100% nucleotide identity) to two human isolates from Egypt, and distant (78-96%) from 16 other HEV isolates from human and animals and shared 99.6% NI with the fourth strain. The consensus sequence of the four strains was origin obtained elsewhere. These data indicated that horses acquire HEV infection and suggest that cross-species transmission may occur. Whether horses play a role in the transmission of HEV needs further investigation.
Asian Journal of Animal and Veterinary Advances, 2008
Journal of Neurosurgery, 2017
OBJECTIVEMeningiomas express somatostatin receptor subtype 2 (SST2), which is targeted by the som... more OBJECTIVEMeningiomas express somatostatin receptor subtype 2 (SST2), which is targeted by the somatostatin analog octreotide. However, to date, using somatostatin analog therapy for the treatment of these tumors in clinical practice has been debated. This study aims to clarify the in vitro effects of octreotide on meningiomas for precise clinical applications.METHODSThe effects of octreotide were analyzed in a large series of 80 meningiomas, including 31 World Health Organization (WHO) Grade II and 4 WHO Grade III tumors, using fresh primary cell cultures to study the impact on cell viability, apoptosis, and signal transduction pathways.RESULTSSST2 mRNA was detected in 100% of the tested meningiomas at levels similar to those observed in other SST2-expressing tumors, neuroendocrine tumors, or pituitary adenomas. Octreotide significantly decreased cell proliferation in 88% of meningiomas but did not induce cell death. On average, cell proliferation was more inhibited in the meningiom...
Assiut University Journal of Multidisciplinary Scientific Research
The Journal of Infection in Developing Countries, 2015
Introduction: Shigella flexneri is a Gram-negative bacteria that has the ability to invade the ep... more Introduction: Shigella flexneri is a Gram-negative bacteria that has the ability to invade the epithelium of the colon and cause colon ulcers. Methodology: The ability of isolated Shigella flexneri from bloody diarrhea to cause colon ulcers was investigated by histopathological examination via oral administration of the bacteria to adult male albino Sprague-Dawley rats. The antibacterial activity of thyme oil, ciprofloxacin, and their combination were evaluated in vitro and in vivo. Results: Oral administration of 12 × 108 CFU/mL of S. flexneri was able to cause colon ulcers. Thyme oil had the highest antibacterial activity among other investigated oils (minimum inhibitory concentration [MIC] 150 µL/L). Ciprofloxacin had the highest antimicrobial activity against S. flexneri (MIC 0.4 mg/L). The synergism between thyme oil and ciprofloxacin showed the maximum growth inhibition of S. flexneri. The synergistic activity of thyme oil and ciprofloxacin succeeded in healing the epithelial ...
Journal of the Egyptian Society of Parasitology, 2007
The reaction of the haemolymph and the tissue of infected intermediate hosts, Biomphalaria alexan... more The reaction of the haemolymph and the tissue of infected intermediate hosts, Biomphalaria alexandrina and Bulinus truncatus to Schistosoma mansoni and S. haematobium antigens were investigated using the indirect immunoperoxidase technique. A new technique, Agarose cell block was used in collection of haemolymph which helped in collecting plenty of well formed cells in comparison to the ordinary one using the cytospin. Collected haemolymph and prepared tissues of uninfected and infected B. alexandria and B. truncatus were fixed and then reacted with anti-S. mansoni and anti-S. haematobium IgG polyclonal antibodies. The haemolymph and tissue of infected B. alexandrina and B. truncatus gave a positive peroxidase reaction represented by a brown colour. In haemolymph, the positive peroxidase reaction was detected mainly in the cytoplasm of the amoebocytes. In the tissue, it was detected in epithelial cells lining the tubules, male cells in the lumen of the tubules and in female oogonia ...
MedGenMed : Medscape general medicine, Jan 23, 2007
The activity of a novel enaminone derivative of 4-hydroxyquinoline, BDHQ, was screened for its ef... more The activity of a novel enaminone derivative of 4-hydroxyquinoline, BDHQ, was screened for its effectiveness against murine schistosomiasis by electron microscopy and parasitologic studies. The correlation of these studies with serum levels of IFN-gamma and IgE is described. Two groups of 10 mice each were treated with different doses of BDHQ, and their results were correlated with the control and praziquantel (PZQ)-treated groups. Parasitologic study revealed significant reduction in mature worms and tissue egg loads in BDHQ- and PZQ-treated groups, whereas immature worms revealed significant reduction in BDHQ groups only. The group treated with a higher dose of BDHQ showed significant reductions in intestinal ova count when compared with the PZQ-treated group. Ultrastructural examination of the worm revealed significant degeneration of the spines and tegument in all treated groups, while the genital system was affected in BDHQ-treated groups only. BDHQ showed considerable effect o...
Journal of the Egyptian Society of Parasitology, 2006
The effect of cyclooxygenase-2 (COX-2) inhibitor, such (as meloxicam, and pyocyanin pigment of Ps... more The effect of cyclooxygenase-2 (COX-2) inhibitor, such (as meloxicam, and pyocyanin pigment of Pseudomonas aeruginosa) with and without praziquantel (PZQ) on worms, ova count, bone marrow and blood cells in 7 groups of Schistosoma mansoni infected mice was studied. The results revealed significant decrease of worm burden and ova count in all treated groups as compared to the infected untreated group, while those with combined treatment of PZQ and meloxicam or pyocyanin showed complete eradication of the worm with the highest reduction in the tissue egg load. EM showed extensive swelling and vesiculation of the tegument, completely implanted spines that overlie degenerated muscle layer were obvious in groups treated with either meloxicam or pyocyanin. Hematological study revealed significant increase (P<0.05) of total leucocytic count of PZQ treated group while that treated with either meloxicam or pyocyanin showed significant decrease (P<0.05), but in combination of PZQ with m...
Journal of the Egyptian Society of Parasitology, 2014
The Scientific World Journal, 2012
The present experiment was conducted to study the effect of ethanolic extract of Egyptian propoli... more The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivatedPasteurella multocidavaccine on rabbits challenged with a virulent strain ofPasteurella multocida. Fifty-six New-Zealand rabbits, 6–8 weeks old and non-vaccinated against pasteurellosis, were randomly divided into eight equal groups. The first group was kept as a control for the experiment. The other groups received different treatments with propolis extract, inactivated vaccine, or both. The experiment continued for seven weeks during which clinical signs, body weight, and mortality rate were monitored, and blood samples were collected weekly for evaluating the leukogram, serum biochemistry, and immune response in all groups of animals. At the end of the seventh week, the animals were subjected to challenge with a virulent strain ofPasteurella multocida. Two weeks later, tissue specimens were collected from different organs for histopathol...
International Journal of Stem Cells, 2014
BioMed Research International, 2013
The present study was conducted to study the protective effect of ethanolic extract of propolis g... more The present study was conducted to study the protective effect of ethanolic extract of propolis given subcutaneously (S/C) either alone or in combination with inactivated formalizedPasteurella multocida(P. multocida) vaccine in rabbits challenged with virulentP. multocidastrain. Twenty-eight New-Zealand rabbits, 6–8 weeks old and not vaccinated against pasteurellosis, were randomly divided into four equal groups. Group (1) was kept as nonvaccinated control. Group (2) was injected S/C with propolis. Group (3) was vaccinated (S/C) withP. multocidavaccine only. Group (4) was injected with vaccine mixed with propolis as adjuvant. Groups (2, 3, and 4) received the same doses of propolis and vaccine after 4 weeks as a booster dose. The experiment continued for six weeks during which clinical signs, body weight, and mortality rate were recorded. Blood samples were collected every 2 weeks of treatment for evaluating the erythrogram and biochemical parameters. At the end of six weeks, all gr...
Journal of Men's Health, 2009
journal of men&#x27;s health, Volume 6, Issue 3, Pages 230, September 2009, Authors:Amira... more journal of men&#x27;s health, Volume 6, Issue 3, Pages 230, September 2009, Authors:Amira Helmy Mohamed; Olfat Ali Hammam; Tarek Ramzy El Lithy; Mohamed Mohi El Din Wishahi.
Infection, Genetics and Evolution, 2007
Hepatitis E virus (HEV) is an important cause of hepatitis among young Egyptian adults with high ... more Hepatitis E virus (HEV) is an important cause of hepatitis among young Egyptian adults with high seroprevalence rates seen in both rural areas of the Nile Delta and in suburban Cairo. Because natural antibodies to HEV have been detected in animals and zoonotic transmission is postulated, we surveyed work horses in Cairo for evidence of HEV exposure and viremia. Sera from 200 Cairo work horses were tested by ELISA for the presence of IgG anti-HEV antibody revealed a seropositivity of 13%. Among 100 samples processed for detection of viral genome by means of nested polymerase chain reaction (N-PCR), 4% were positive and indicative of viremia. Viremic animals were less than 1 year old. Relative to PCR-negative horses, PCR-positive animals demonstrated significant elevation of AST (p=0.03). Phylogenetic analysis of a 253-bp fragment, in the ORF-1,2,3 overlap region of the HEV genome from the viremic animals showed that three of these viral strains to be identical, and closely related (97-100% nucleotide identity) to two human isolates from Egypt, and distant (78-96%) from 16 other HEV isolates from human and animals and shared 99.6% NI with the fourth strain. The consensus sequence of the four strains was origin obtained elsewhere. These data indicated that horses acquire HEV infection and suggest that cross-species transmission may occur. Whether horses play a role in the transmission of HEV needs further investigation.
Asian Journal of Animal and Veterinary Advances, 2008
Journal of Neurosurgery, 2017
OBJECTIVEMeningiomas express somatostatin receptor subtype 2 (SST2), which is targeted by the som... more OBJECTIVEMeningiomas express somatostatin receptor subtype 2 (SST2), which is targeted by the somatostatin analog octreotide. However, to date, using somatostatin analog therapy for the treatment of these tumors in clinical practice has been debated. This study aims to clarify the in vitro effects of octreotide on meningiomas for precise clinical applications.METHODSThe effects of octreotide were analyzed in a large series of 80 meningiomas, including 31 World Health Organization (WHO) Grade II and 4 WHO Grade III tumors, using fresh primary cell cultures to study the impact on cell viability, apoptosis, and signal transduction pathways.RESULTSSST2 mRNA was detected in 100% of the tested meningiomas at levels similar to those observed in other SST2-expressing tumors, neuroendocrine tumors, or pituitary adenomas. Octreotide significantly decreased cell proliferation in 88% of meningiomas but did not induce cell death. On average, cell proliferation was more inhibited in the meningiom...