anshu singh - Academia.edu (original) (raw)

Papers by anshu singh

Research paper thumbnail of Sett Priming with Plant Growth Promoting Chemicals Affecting Germination and Physio-Biochemical Attributes in Sugarcane

Climate Change and Environmental Sustainability, 2018

Research paper thumbnail of Assessment of diabetes risk in an adult population using Indian diabetes risk score in rural area of Chennai

International Journal of Advanced Community Medicine, 2019

Epidemiological transitions in India in the 21 st century have led to non communicable diseases b... more Epidemiological transitions in India in the 21 st century have led to non communicable diseases becoming a major public health problem of growing magnitude. Diabetes is an iceberg disease. Most of the cases remain asymptomatic. Screening for diabetes can identify patients at an early stage of the disease. A community-based, cross-sectional study was carried out in the rural field practise area of A.C.S. Medical College and hospital (Nazarathpet) in 2018 and analyzed by SPSS 16.0 version. Out of 305 subjects, 30.5% were in low risk category, 52.5% were in moderate risk and 17.0% were at high risk group as per Indian Diabetes Risk Score. Majority of the study subjects were at risk of having diabetes.

Research paper thumbnail of Contribution of NTFPs in the Livelihood of Mangrove Forest Dwellers of Sundarban

Journal of Human Ecology, 2010

Sundarbans are the world's largest mangrove biomes having highest mangrove biodiversity, which ar... more Sundarbans are the world's largest mangrove biomes having highest mangrove biodiversity, which are used for subsistence and commercial purpose by the local inhabitants. It is one of the essential components of the livelihood of the forest dependant population of the area. The paper tries to explore the spectrum of NTFPs being collected from Sundarbans and its contribution in the livelihood of the people. It also attempts to assess the livelihood assets of the people and how much of it is attributed to NTFPs. It explores the livelihood vulnerability and stress coping mechanisms of the local population and how NTFPs help as a "safety net". For the purpose of the study 4 EDCs from 2 ranges of Sundarbans were sampled, viz: Bally II and Bijoynagar, from Bidya Range; and Dulki and Hamiltanabad (Anpur) from Sajnakhali Wildlife Sanctuary Range. The parameters of the study included: (i) range of NTFPs being collected; (ii) seasonality and duration of collection; (iii) method of collection; (iv) extent of dependence; (v) total household income and contribution of NTFPs. The respondents were selected from different occupation class and income groups. The study shows that the contribution of NTFPs is quite high as it contributes almost 79% (Rs. 80,000) on an average to the annual income of the collector's family. Of course not every family of the village goes for NTFP collection but nonetheless their number is significant. The major NTFP that are being collected includes firewood, prawn, fishes, crab, honey and bee wax. The collection of NTFPs is a daunting task, which involves risk from man-eater tigers. High livelihood vulnerability was also observed with little help from government. If, these biomes are to be conserved it is necessary that Sundarban Development Authority must consider the role of NTFPs in the livelihood and develop the Sustainable Livelihood Framework accordingly so that the biomes as well as people's need could be sustained.

Research paper thumbnail of Biotransformation of hydrolysable tannin to ellagic acid by tannase from Aspergillus awamori

Biocatalysis and Biotransformation, 2017

Madhuca indica, locally known as mahua in India is a multipurpose tree species. Mahua, particular... more Madhuca indica, locally known as mahua in India is a multipurpose tree species. Mahua, particularly bark contains a significant amount of hydrolysable tannin (17.31%) which can be utilized for ellagic acid production through biotransformation. In the present study, mahua bark utilized not only as a raw material for tannase production but also for ellagic acid a well-known therapeutic compound. After prior confirmation of hydrolysable tannin content in bark, it has been supplemented, as a substrate for tannase production through solid state fermentation of Aspergillus awamori. Tannase production, as well as biodegradation of the hydrolysable tannin reached a maximum at 72 h of incubation time. The optimum conditions for tannase production are solid to liquid ratio of 1:2, 35 C, pH 5.5 and 72h incubation time which resulted 0.256 mg/mL of an extract of ellagic acid. Maximum tannase activity of 56.16 IU/gds at 35 C and 72h of incubation time is recorded. It seems that tannase production and biotransformation of hydrolysable tannins using bark powder of mahua can be considered as an appropriate alternative to the existing procedures of ellagic acid production.

Research paper thumbnail of Present Status on Enzymatic Hydrolysis of Lignocellulosic Biomass for Bioethanol Production

Advances in Biofeedstocks and Biofuels, 2017

Research paper thumbnail of Changes in Growth, Yield, Juice Quality and Biochemical Attributes of Sugarcane in Response to Orthosilicic Acid Granules

Sugar Tech, 2016

A field experiment was conducted to study the effect of silica granules in the form of orthosilic... more A field experiment was conducted to study the effect of silica granules in the form of orthosilicic acid on growth, yield and juice quality attributes of sugarcane variety CoLk 94184 at IISR experimental farm, Lucknow. The treatments comprised of control (T1), orthosilicic granules @20 (T2), 40 (T3) and 80 kg/ha (T4) and calcium silicate @2 t/ha (T5). Silica application showed higher shoot population, specific leaf weight and total dry matter accumulation relative to control. Cane girth, cane height and cane yield were increased due to silica application. Highest increase in these traits was obtained in T3 treatment. Juice quality attributes, viz.°Brix, sucrose percent juice, juice purity, juice extraction, CCS percent juice, S/ R ratio and SPS activity, were higher in silica-treated plants relative to control. CCS increase was recorded about 15.2-31.8 % over control with highest increase (31.8 %) in T4 treatment. Soluble silica content was significantly higher in leaf and root tissues of treated plants. Findings suggest that application of orthosilicic acid may be beneficial in improving cane yield and juice quality of sugar cane.

Research paper thumbnail of Development of Calibration Curves for Quick Tests Used in Estimating Nutrients from Dairy Wastes in Kentucky

2004, Ottawa, Canada August 1 - 4, 2004, 2004

The authors are solely responsible for the content of this technical presentation. The technical ... more The authors are solely responsible for the content of this technical presentation. The technical presentation does not necessarily reflect the official position of ASAE, and its printing and distribution does not constitute an endorsement of views which may be expressed. Technical ...

Research paper thumbnail of Influence of selenium on metallothionein gene expression and physiological characteristics of sugarcane plants

Plant Growth Regulation, 2015

Selenium (Se), one of the most widely distributed elements of the earth's crust, is required in t... more Selenium (Se), one of the most widely distributed elements of the earth's crust, is required in trace amounts for normal growth and development of biological activity but its increasing level in soil poses productivity problems in many crops including sugarcane. In the present investigation, a promising line of sugarcane (CoLk 94184) was used to assess the impact of selenium on growth, physio-biochemical attributes vis-à-vis expression of metallothionein (MT) gene. Single bud setts of sugarcane (Saccharum spp. hybrids) was planted with differential levels of selenium (sodium selenite) viz., 0, 10, 50 and 100 ppm under soil tray culture conditions. At higher concentrations (50 and 100 ppm Se), symptoms of metal toxicity as stunted growth, reduced plant height, vigor, root, shoot weight and leaf chlorosis were observed. Biochemical analysis revealed reduction in content of chlorophylls, carotenoids, proline and induction of lipid peroxidation in terms of malondialdehyde content and higher activity of peroxidase enzyme. qRT-PCR analysis indicated increase in expression of MT gene in leaf tissue with an increase in Se supply and highest expression was observed at 50 ppm Se. At 100 ppm supply, adverse effect of Se was very severe and a minor increase in expression of MT gene was observed. Results suggest that MT gene is related to the Se homeostasis which in turn helps in tolerance to Se toxicity in sugarcane.

Research paper thumbnail of Significance of upper airway influence among patients of vocal cord dysfunction for its diagnosis: Role of impulse oscillometry

Lung India, 2009

Original Article Background: To identify the patients of bronchial asthma (suspected or proven), ... more Original Article Background: To identify the patients of bronchial asthma (suspected or proven), not responding to optimal therapy, for the presence of vocal cord dysfunction (VCD) and to compare the diagnostic ability of fl ow volume (FV) loop and impulse oscillometry (IOS). Materials and Methods: Fifty one patients of suspected/proven bronchial asthma not responding to optimal therapy were included for the study. Each patient was subjected to both FV loop and IOS studies. Direct visualization of the vocal cords with fl exible fi beroptic bronchoscope for the presence of inspiratory vocal cord adduction during quiet respiration, with speech, and while performing provocative maneuvers was carried out. All patients were subjected to simple pulmonary function tests and recording of FV loop. IOS was performed on each patient to look for the site of obstruction and upper airway infl uence. The observations of both FV loop and IO studies were compared. Results: Among 51 patients participated, 12 (23.53%) had bronchoscopical evidence of VCD and were labeled as VCD-positive group and rest 39 were designated VCD negative. No statistically signifi cant difference in pulmonary function test (prereversibility) results between the VCD-positive and VCD-negative patients was found. Reversible airway obstruction was observed in 75% of the patients of VCD-positive group and 67.65% of the patients in the VCD-negative group. Only one patient in the VCD-positive and none in VCD-negative group had inspiratory limb fl attening of FV loop. Upper airway infl uence was evident by IOS in 58.3% of patients in the VCD-positive group and in 15.4% of patients in the VCD-negative group. This difference was statistically signifi cant (P < 0.005). Conclusion: VCD was a common fi nding in patients with symptoms suggestive of asthma and frequently coexists with asthma. IOS was found to be a useful screening test for VCD and was more sensitive than FV loop.

Research paper thumbnail of Utilization of Vegetable Wastes for Bioenergy Generation

Agricultural Research, 2012

Vegetable wastes occur throughout the supply chain and vary widely depending on its processing. G... more Vegetable wastes occur throughout the supply chain and vary widely depending on its processing. Globally, more than 30 % of the loss occurs at the retail and consumer levels, of which the post-harvest and processing level wastages account for the major share. The wastes so generated pose an environmental threat and call for the development of a pollution-free model. Studies on the characterization of unutilized, rotten, and discarded fractions of the vegetable wastes indicate their potential candidature for reprocessing. Generation of renewable energy by bioconversion of vegetable wastes is gaining importance as it has proved to be a proficient means of utilizing the perishable vegetable residues. Researchers and industries are now fully engaged in a number of projects involving the technology of ''waste to fuel'' with a view to overcome the disposal problems. The present article deals with the studies conducted on vegetable wastes for production of various types of biofuel.

Research paper thumbnail of Enzymatic polishing of cereal grains for improved nutrient retainment

Journal of Food Science and Technology, 2014

Consumer acceptance of food products is largely driven by the dietary and functional quality of t... more Consumer acceptance of food products is largely driven by the dietary and functional quality of their ingredients. Though whole cereal grains are well known for bioactive components, scientists are facing dire need for better technologies to prevent the nutritional losses incurred through the conventional food processing technologies. Application of enzyme for depolymerisation of carbohydrates present in bran layer of grain is becoming an efficient method for phenolic mobilization and dietary fiber solubilisation. The present article emphasizes deep insights about the application of enzyme as an alternative technology for cereal grain processing to improve the product quality while forbidding the nutritional losses in an eco-friendly manner.

Research paper thumbnail of Process optimization for aqueous extraction of reducing sugar from cashew apple bagasse: A potential, low cost substrate

LWT - Food Science and Technology, 2011

The present investigation deals with optimization of aqueous extraction of reducing sugar from ca... more The present investigation deals with optimization of aqueous extraction of reducing sugar from cashew apple bagaase, a potential low cost substrate. Response Surface Methodology (RSM) based Box Behnken Design (BBD) was employed to obtain the best possible combination of liquid: solid, pH, extraction time and extraction temperature for maximum reducing sugar extraction. The optimum extraction conditions were as follows: liquid: solid 3.26 (mL/g), pH 6.42, extraction time 6.30 h and temperature 52.27 C. Under these conditions, the experimental yield was 56.89 (g/100 g dry substrate), which was well matched with the predictive yield 57.64 (g/100 g dry substrate). Further analysis of sugar was done by HPLC which revealed glucose (34.28 g/100 g dry substrate), fructose (18.57 g/100 g dry substrate) and arabinose (3.42 g/100 g dry substrate).

Research paper thumbnail of Suprascapular neuropathy secondary to reverse shoulder arthroplasty: A case report

Journal of Shoulder and Elbow Surgery, 2010

Suprascapular neuropathy (SSN) is a relatively rare cause of shoulder pain that is usually made a... more Suprascapular neuropathy (SSN) is a relatively rare cause of shoulder pain that is usually made as a diagnosis of exclusion. Reported causes of SSN include compression from adjacent ganglia, 7,8,12,13 abnormal morphology of the suprascapular notch, 15,17 neuritis, 11 direct trauma or traction injury, 10,20 massive rotator cuff tear, 2,18 and iatrogenic injury. 4,16,18 We present a case of SSN as a consequence of errant superior screw placement during revision reverse shoulder arthroplasty. Case report A 55-year-old man had had 5 prior surgeries for rotator cuff repair which had failed and had then undergone a reverse prosthesis. Due to an infection following this procedure, he then underwent resection of the prosthesis and placement of an antibiotic-impregnated spacer (prostalac) device. The prostalac device fractured after 2 months and the subsequent infection work-up showed that ESR and CRP were 54 mm/hr and 0.6 mg/l, respectively. After 3 more months, the infection work up demonstrated all negative infection markers (ESR, CRP, cultures from aspiration), so an inverse shoulder replacement was

Research paper thumbnail of Ultrasound-Guided Interscalene Block Anesthesia for Shoulder Arthroscopy

The Journal of Bone and Joint Surgery-American Volume, 2012

Background: Ultrasound guidance improves the localization of anesthetic placement during regional... more Background: Ultrasound guidance improves the localization of anesthetic placement during regional anesthesia, but a decreased rate of adverse events has not been demonstrated in the current literature. In this large prospective study, we evaluated the safety, efficacy, and patient satisfaction associated with ultrasound-guided interscalene block. Methods: A cohort of 1319 patients undergoing arthroscopic shoulder surgery at an outpatient surgery center was prospectively evaluated. Interscalene blocks were performed by experienced anesthesiologists and trainees with use of ultrasound guidance. Patients were queried by a physician twenty-four hours postoperatively regarding their satisfaction with the interscalene block and were screened for a comprehensive register of minor and major adverse events. Individuals with adverse events were followed until symptoms resolved. Results: Interscalene block was ultimately successful in 99.6% of the cases. A total of thirty-eight adverse events (prevalence, 2.88%) were noted. At the time of the latest follow-up, permanent sequelae were present in three patients (0.23%), all of whom had relevant comorbidities. With regard to patient satisfaction, 99.06% of the respondents were ''satisfied'' or ''very satisfied'' with the interscalene block, whereas 0.94% of respondents were unsatisfied. In addition, 97.8% of the patients stated that they would elect to have an interscalene block again in the future. Conclusions: The present study supports the use of ultrasound-guided interscalene block by trained anesthesiologists for well-screened patients undergoing shoulder arthroscopy, given the high rate of patient satisfaction and the low rate of adverse events.

Research paper thumbnail of Runoff and drainage water quality from geotextile and gravel pads used in livestock feeding and loafing areas

Bioresource Technology, 2008

Geotextile and gravel pads offer a low-cost alternative to concrete for providing all-weather sur... more Geotextile and gravel pads offer a low-cost alternative to concrete for providing all-weather surfaces for cattle and vehicle traffic, and are used in many livestock facilities to minimize mud, runoff and erosion of heavy traffic areas. The objective of this study was to compare different combinations of geotextile and gravel used in heavy livestock traffic areas that minimize the potential for water pollution. Three different pad combinations were constructed in 2.4 • 6-m plots as follows: (i) woven geotextile + 100 mm of gravel + 50 mm Dense Grade Aggregate (DGA); (ii) woven geotextile + geoweb Ò + 100 mm DGA; and (iii) non-woven geotextile + 152 mm of gravel + 50 mm DGA; (iv) mud lots as control. The third combination was equivalent to one of the base treatments specified by the Kentucky Natural Resource and Conservation Service (NRCS). All treatment combinations were duplicated. Lysimeter pans were installed in four out of eight plots for the collection of leachate or drainage water. Runoff was collected at the lower end of the plots. About 14 kg of beef cattle manure were added evenly to the plots. Rainfall at 50 mm/h was applied using rainfall simulators. In the first five of ten experiments, manure was removed from the surface of the pads after each experiment. In the remaining five experiments manure accumulated on the surface of the pads. The effect of pad treatment was significant on the electrical conductivity (EC), total solids (TS), chemical oxygen demand (COD), nitrite (NO 2-N), total nitrogen (TN) and total phosphorus (TP) values in surface runoff at the 5% level. Manure removal did not have any significant effect on the nutrient content of runoff or leachate samples except for ammonia (NH 4-N) values. Although a mass balance indicated relatively small amounts of organic matter and nutrients were lost by runoff and leaching, the actual contamination level of both runoff and leachate samples were high; TP levels as high as 12 mg/l (5.4 mg/m 2) in runoff and nitrate (NO 3-N) values as high as 10.8 mg/l (1.6 mg/m 2) in leachate were observed.

Research paper thumbnail of Comparative analysis of solid-state bioprocessing and enzymatic treatment of finger millet for mobilization of bound phenolics

Bioprocess and Biosystems Engineering, 2013

The present work investigates the probable bioprocessing technique to mobilize the bound phenolic... more The present work investigates the probable bioprocessing technique to mobilize the bound phenolics naturally found in finger millet cell wall for enriching it with dietary antioxidants. Comparative study was performed between the exogenous enzymatic treatment and solid-state fermentation of grain (SSF) with a food grade organism Rhizopus oryzae. SSF results indicated that at the 6th day of incubation, total phenolic content (18.64 mg gallic acid equivalent/gds) and antioxidant property (DPPH radical scavenging activity of 39.03 %, metal chelating ability of 54 % and better reducing power) of finger millet were drastically enhanced when fermented with GRAS filamentous fungi. During the enzymatic bioprocessing, most of the phenolics released during the hydrolysis, leached out into the liquid portion rather than retaining them within the millet grain, resulting in overall loss of dietary antioxidant. The present study establishes the most effective strategy to enrich the finger millet with phenolic antioxidants.

Research paper thumbnail of Optimiranje ekstrakcije polifenola iz okare

Food Technology and Biotechnology, Aug 10, 2011

Sažetak Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti... more Sažetak Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti održivi, ekološki postupak izdvajanja maksimalne količine polifenola. Da bi se ispitao utjecaj temperature, udjela otapala i vremena inkubacije na ekstrakciju polifenola, upotrijebljen je Box-Behnken dizajn metodologije odzivnih površina uz pomoć softvera MINITAB 15. Upotrijebljen je aceton kao otapalo za ekstrakciju fenolnih spojeva, te su ispitana svojstva polifenola, i to: sposobnost uklanjanja DPPH radikala, reducirajuća snaga i aktivnost ...

Research paper thumbnail of Sett Priming with Plant Growth Promoting Chemicals Affecting Germination and Physio-Biochemical Attributes in Sugarcane

Climate Change and Environmental Sustainability, 2018

Research paper thumbnail of Assessment of diabetes risk in an adult population using Indian diabetes risk score in rural area of Chennai

International Journal of Advanced Community Medicine, 2019

Epidemiological transitions in India in the 21 st century have led to non communicable diseases b... more Epidemiological transitions in India in the 21 st century have led to non communicable diseases becoming a major public health problem of growing magnitude. Diabetes is an iceberg disease. Most of the cases remain asymptomatic. Screening for diabetes can identify patients at an early stage of the disease. A community-based, cross-sectional study was carried out in the rural field practise area of A.C.S. Medical College and hospital (Nazarathpet) in 2018 and analyzed by SPSS 16.0 version. Out of 305 subjects, 30.5% were in low risk category, 52.5% were in moderate risk and 17.0% were at high risk group as per Indian Diabetes Risk Score. Majority of the study subjects were at risk of having diabetes.

Research paper thumbnail of Contribution of NTFPs in the Livelihood of Mangrove Forest Dwellers of Sundarban

Journal of Human Ecology, 2010

Sundarbans are the world's largest mangrove biomes having highest mangrove biodiversity, which ar... more Sundarbans are the world's largest mangrove biomes having highest mangrove biodiversity, which are used for subsistence and commercial purpose by the local inhabitants. It is one of the essential components of the livelihood of the forest dependant population of the area. The paper tries to explore the spectrum of NTFPs being collected from Sundarbans and its contribution in the livelihood of the people. It also attempts to assess the livelihood assets of the people and how much of it is attributed to NTFPs. It explores the livelihood vulnerability and stress coping mechanisms of the local population and how NTFPs help as a "safety net". For the purpose of the study 4 EDCs from 2 ranges of Sundarbans were sampled, viz: Bally II and Bijoynagar, from Bidya Range; and Dulki and Hamiltanabad (Anpur) from Sajnakhali Wildlife Sanctuary Range. The parameters of the study included: (i) range of NTFPs being collected; (ii) seasonality and duration of collection; (iii) method of collection; (iv) extent of dependence; (v) total household income and contribution of NTFPs. The respondents were selected from different occupation class and income groups. The study shows that the contribution of NTFPs is quite high as it contributes almost 79% (Rs. 80,000) on an average to the annual income of the collector's family. Of course not every family of the village goes for NTFP collection but nonetheless their number is significant. The major NTFP that are being collected includes firewood, prawn, fishes, crab, honey and bee wax. The collection of NTFPs is a daunting task, which involves risk from man-eater tigers. High livelihood vulnerability was also observed with little help from government. If, these biomes are to be conserved it is necessary that Sundarban Development Authority must consider the role of NTFPs in the livelihood and develop the Sustainable Livelihood Framework accordingly so that the biomes as well as people's need could be sustained.

Research paper thumbnail of Biotransformation of hydrolysable tannin to ellagic acid by tannase from Aspergillus awamori

Biocatalysis and Biotransformation, 2017

Madhuca indica, locally known as mahua in India is a multipurpose tree species. Mahua, particular... more Madhuca indica, locally known as mahua in India is a multipurpose tree species. Mahua, particularly bark contains a significant amount of hydrolysable tannin (17.31%) which can be utilized for ellagic acid production through biotransformation. In the present study, mahua bark utilized not only as a raw material for tannase production but also for ellagic acid a well-known therapeutic compound. After prior confirmation of hydrolysable tannin content in bark, it has been supplemented, as a substrate for tannase production through solid state fermentation of Aspergillus awamori. Tannase production, as well as biodegradation of the hydrolysable tannin reached a maximum at 72 h of incubation time. The optimum conditions for tannase production are solid to liquid ratio of 1:2, 35 C, pH 5.5 and 72h incubation time which resulted 0.256 mg/mL of an extract of ellagic acid. Maximum tannase activity of 56.16 IU/gds at 35 C and 72h of incubation time is recorded. It seems that tannase production and biotransformation of hydrolysable tannins using bark powder of mahua can be considered as an appropriate alternative to the existing procedures of ellagic acid production.

Research paper thumbnail of Present Status on Enzymatic Hydrolysis of Lignocellulosic Biomass for Bioethanol Production

Advances in Biofeedstocks and Biofuels, 2017

Research paper thumbnail of Changes in Growth, Yield, Juice Quality and Biochemical Attributes of Sugarcane in Response to Orthosilicic Acid Granules

Sugar Tech, 2016

A field experiment was conducted to study the effect of silica granules in the form of orthosilic... more A field experiment was conducted to study the effect of silica granules in the form of orthosilicic acid on growth, yield and juice quality attributes of sugarcane variety CoLk 94184 at IISR experimental farm, Lucknow. The treatments comprised of control (T1), orthosilicic granules @20 (T2), 40 (T3) and 80 kg/ha (T4) and calcium silicate @2 t/ha (T5). Silica application showed higher shoot population, specific leaf weight and total dry matter accumulation relative to control. Cane girth, cane height and cane yield were increased due to silica application. Highest increase in these traits was obtained in T3 treatment. Juice quality attributes, viz.°Brix, sucrose percent juice, juice purity, juice extraction, CCS percent juice, S/ R ratio and SPS activity, were higher in silica-treated plants relative to control. CCS increase was recorded about 15.2-31.8 % over control with highest increase (31.8 %) in T4 treatment. Soluble silica content was significantly higher in leaf and root tissues of treated plants. Findings suggest that application of orthosilicic acid may be beneficial in improving cane yield and juice quality of sugar cane.

Research paper thumbnail of Development of Calibration Curves for Quick Tests Used in Estimating Nutrients from Dairy Wastes in Kentucky

2004, Ottawa, Canada August 1 - 4, 2004, 2004

The authors are solely responsible for the content of this technical presentation. The technical ... more The authors are solely responsible for the content of this technical presentation. The technical presentation does not necessarily reflect the official position of ASAE, and its printing and distribution does not constitute an endorsement of views which may be expressed. Technical ...

Research paper thumbnail of Influence of selenium on metallothionein gene expression and physiological characteristics of sugarcane plants

Plant Growth Regulation, 2015

Selenium (Se), one of the most widely distributed elements of the earth's crust, is required in t... more Selenium (Se), one of the most widely distributed elements of the earth's crust, is required in trace amounts for normal growth and development of biological activity but its increasing level in soil poses productivity problems in many crops including sugarcane. In the present investigation, a promising line of sugarcane (CoLk 94184) was used to assess the impact of selenium on growth, physio-biochemical attributes vis-à-vis expression of metallothionein (MT) gene. Single bud setts of sugarcane (Saccharum spp. hybrids) was planted with differential levels of selenium (sodium selenite) viz., 0, 10, 50 and 100 ppm under soil tray culture conditions. At higher concentrations (50 and 100 ppm Se), symptoms of metal toxicity as stunted growth, reduced plant height, vigor, root, shoot weight and leaf chlorosis were observed. Biochemical analysis revealed reduction in content of chlorophylls, carotenoids, proline and induction of lipid peroxidation in terms of malondialdehyde content and higher activity of peroxidase enzyme. qRT-PCR analysis indicated increase in expression of MT gene in leaf tissue with an increase in Se supply and highest expression was observed at 50 ppm Se. At 100 ppm supply, adverse effect of Se was very severe and a minor increase in expression of MT gene was observed. Results suggest that MT gene is related to the Se homeostasis which in turn helps in tolerance to Se toxicity in sugarcane.

Research paper thumbnail of Significance of upper airway influence among patients of vocal cord dysfunction for its diagnosis: Role of impulse oscillometry

Lung India, 2009

Original Article Background: To identify the patients of bronchial asthma (suspected or proven), ... more Original Article Background: To identify the patients of bronchial asthma (suspected or proven), not responding to optimal therapy, for the presence of vocal cord dysfunction (VCD) and to compare the diagnostic ability of fl ow volume (FV) loop and impulse oscillometry (IOS). Materials and Methods: Fifty one patients of suspected/proven bronchial asthma not responding to optimal therapy were included for the study. Each patient was subjected to both FV loop and IOS studies. Direct visualization of the vocal cords with fl exible fi beroptic bronchoscope for the presence of inspiratory vocal cord adduction during quiet respiration, with speech, and while performing provocative maneuvers was carried out. All patients were subjected to simple pulmonary function tests and recording of FV loop. IOS was performed on each patient to look for the site of obstruction and upper airway infl uence. The observations of both FV loop and IO studies were compared. Results: Among 51 patients participated, 12 (23.53%) had bronchoscopical evidence of VCD and were labeled as VCD-positive group and rest 39 were designated VCD negative. No statistically signifi cant difference in pulmonary function test (prereversibility) results between the VCD-positive and VCD-negative patients was found. Reversible airway obstruction was observed in 75% of the patients of VCD-positive group and 67.65% of the patients in the VCD-negative group. Only one patient in the VCD-positive and none in VCD-negative group had inspiratory limb fl attening of FV loop. Upper airway infl uence was evident by IOS in 58.3% of patients in the VCD-positive group and in 15.4% of patients in the VCD-negative group. This difference was statistically signifi cant (P < 0.005). Conclusion: VCD was a common fi nding in patients with symptoms suggestive of asthma and frequently coexists with asthma. IOS was found to be a useful screening test for VCD and was more sensitive than FV loop.

Research paper thumbnail of Utilization of Vegetable Wastes for Bioenergy Generation

Agricultural Research, 2012

Vegetable wastes occur throughout the supply chain and vary widely depending on its processing. G... more Vegetable wastes occur throughout the supply chain and vary widely depending on its processing. Globally, more than 30 % of the loss occurs at the retail and consumer levels, of which the post-harvest and processing level wastages account for the major share. The wastes so generated pose an environmental threat and call for the development of a pollution-free model. Studies on the characterization of unutilized, rotten, and discarded fractions of the vegetable wastes indicate their potential candidature for reprocessing. Generation of renewable energy by bioconversion of vegetable wastes is gaining importance as it has proved to be a proficient means of utilizing the perishable vegetable residues. Researchers and industries are now fully engaged in a number of projects involving the technology of ''waste to fuel'' with a view to overcome the disposal problems. The present article deals with the studies conducted on vegetable wastes for production of various types of biofuel.

Research paper thumbnail of Enzymatic polishing of cereal grains for improved nutrient retainment

Journal of Food Science and Technology, 2014

Consumer acceptance of food products is largely driven by the dietary and functional quality of t... more Consumer acceptance of food products is largely driven by the dietary and functional quality of their ingredients. Though whole cereal grains are well known for bioactive components, scientists are facing dire need for better technologies to prevent the nutritional losses incurred through the conventional food processing technologies. Application of enzyme for depolymerisation of carbohydrates present in bran layer of grain is becoming an efficient method for phenolic mobilization and dietary fiber solubilisation. The present article emphasizes deep insights about the application of enzyme as an alternative technology for cereal grain processing to improve the product quality while forbidding the nutritional losses in an eco-friendly manner.

Research paper thumbnail of Process optimization for aqueous extraction of reducing sugar from cashew apple bagasse: A potential, low cost substrate

LWT - Food Science and Technology, 2011

The present investigation deals with optimization of aqueous extraction of reducing sugar from ca... more The present investigation deals with optimization of aqueous extraction of reducing sugar from cashew apple bagaase, a potential low cost substrate. Response Surface Methodology (RSM) based Box Behnken Design (BBD) was employed to obtain the best possible combination of liquid: solid, pH, extraction time and extraction temperature for maximum reducing sugar extraction. The optimum extraction conditions were as follows: liquid: solid 3.26 (mL/g), pH 6.42, extraction time 6.30 h and temperature 52.27 C. Under these conditions, the experimental yield was 56.89 (g/100 g dry substrate), which was well matched with the predictive yield 57.64 (g/100 g dry substrate). Further analysis of sugar was done by HPLC which revealed glucose (34.28 g/100 g dry substrate), fructose (18.57 g/100 g dry substrate) and arabinose (3.42 g/100 g dry substrate).

Research paper thumbnail of Suprascapular neuropathy secondary to reverse shoulder arthroplasty: A case report

Journal of Shoulder and Elbow Surgery, 2010

Suprascapular neuropathy (SSN) is a relatively rare cause of shoulder pain that is usually made a... more Suprascapular neuropathy (SSN) is a relatively rare cause of shoulder pain that is usually made as a diagnosis of exclusion. Reported causes of SSN include compression from adjacent ganglia, 7,8,12,13 abnormal morphology of the suprascapular notch, 15,17 neuritis, 11 direct trauma or traction injury, 10,20 massive rotator cuff tear, 2,18 and iatrogenic injury. 4,16,18 We present a case of SSN as a consequence of errant superior screw placement during revision reverse shoulder arthroplasty. Case report A 55-year-old man had had 5 prior surgeries for rotator cuff repair which had failed and had then undergone a reverse prosthesis. Due to an infection following this procedure, he then underwent resection of the prosthesis and placement of an antibiotic-impregnated spacer (prostalac) device. The prostalac device fractured after 2 months and the subsequent infection work-up showed that ESR and CRP were 54 mm/hr and 0.6 mg/l, respectively. After 3 more months, the infection work up demonstrated all negative infection markers (ESR, CRP, cultures from aspiration), so an inverse shoulder replacement was

Research paper thumbnail of Ultrasound-Guided Interscalene Block Anesthesia for Shoulder Arthroscopy

The Journal of Bone and Joint Surgery-American Volume, 2012

Background: Ultrasound guidance improves the localization of anesthetic placement during regional... more Background: Ultrasound guidance improves the localization of anesthetic placement during regional anesthesia, but a decreased rate of adverse events has not been demonstrated in the current literature. In this large prospective study, we evaluated the safety, efficacy, and patient satisfaction associated with ultrasound-guided interscalene block. Methods: A cohort of 1319 patients undergoing arthroscopic shoulder surgery at an outpatient surgery center was prospectively evaluated. Interscalene blocks were performed by experienced anesthesiologists and trainees with use of ultrasound guidance. Patients were queried by a physician twenty-four hours postoperatively regarding their satisfaction with the interscalene block and were screened for a comprehensive register of minor and major adverse events. Individuals with adverse events were followed until symptoms resolved. Results: Interscalene block was ultimately successful in 99.6% of the cases. A total of thirty-eight adverse events (prevalence, 2.88%) were noted. At the time of the latest follow-up, permanent sequelae were present in three patients (0.23%), all of whom had relevant comorbidities. With regard to patient satisfaction, 99.06% of the respondents were ''satisfied'' or ''very satisfied'' with the interscalene block, whereas 0.94% of respondents were unsatisfied. In addition, 97.8% of the patients stated that they would elect to have an interscalene block again in the future. Conclusions: The present study supports the use of ultrasound-guided interscalene block by trained anesthesiologists for well-screened patients undergoing shoulder arthroscopy, given the high rate of patient satisfaction and the low rate of adverse events.

Research paper thumbnail of Runoff and drainage water quality from geotextile and gravel pads used in livestock feeding and loafing areas

Bioresource Technology, 2008

Geotextile and gravel pads offer a low-cost alternative to concrete for providing all-weather sur... more Geotextile and gravel pads offer a low-cost alternative to concrete for providing all-weather surfaces for cattle and vehicle traffic, and are used in many livestock facilities to minimize mud, runoff and erosion of heavy traffic areas. The objective of this study was to compare different combinations of geotextile and gravel used in heavy livestock traffic areas that minimize the potential for water pollution. Three different pad combinations were constructed in 2.4 • 6-m plots as follows: (i) woven geotextile + 100 mm of gravel + 50 mm Dense Grade Aggregate (DGA); (ii) woven geotextile + geoweb Ò + 100 mm DGA; and (iii) non-woven geotextile + 152 mm of gravel + 50 mm DGA; (iv) mud lots as control. The third combination was equivalent to one of the base treatments specified by the Kentucky Natural Resource and Conservation Service (NRCS). All treatment combinations were duplicated. Lysimeter pans were installed in four out of eight plots for the collection of leachate or drainage water. Runoff was collected at the lower end of the plots. About 14 kg of beef cattle manure were added evenly to the plots. Rainfall at 50 mm/h was applied using rainfall simulators. In the first five of ten experiments, manure was removed from the surface of the pads after each experiment. In the remaining five experiments manure accumulated on the surface of the pads. The effect of pad treatment was significant on the electrical conductivity (EC), total solids (TS), chemical oxygen demand (COD), nitrite (NO 2-N), total nitrogen (TN) and total phosphorus (TP) values in surface runoff at the 5% level. Manure removal did not have any significant effect on the nutrient content of runoff or leachate samples except for ammonia (NH 4-N) values. Although a mass balance indicated relatively small amounts of organic matter and nutrients were lost by runoff and leaching, the actual contamination level of both runoff and leachate samples were high; TP levels as high as 12 mg/l (5.4 mg/m 2) in runoff and nitrate (NO 3-N) values as high as 10.8 mg/l (1.6 mg/m 2) in leachate were observed.

Research paper thumbnail of Comparative analysis of solid-state bioprocessing and enzymatic treatment of finger millet for mobilization of bound phenolics

Bioprocess and Biosystems Engineering, 2013

The present work investigates the probable bioprocessing technique to mobilize the bound phenolic... more The present work investigates the probable bioprocessing technique to mobilize the bound phenolics naturally found in finger millet cell wall for enriching it with dietary antioxidants. Comparative study was performed between the exogenous enzymatic treatment and solid-state fermentation of grain (SSF) with a food grade organism Rhizopus oryzae. SSF results indicated that at the 6th day of incubation, total phenolic content (18.64 mg gallic acid equivalent/gds) and antioxidant property (DPPH radical scavenging activity of 39.03 %, metal chelating ability of 54 % and better reducing power) of finger millet were drastically enhanced when fermented with GRAS filamentous fungi. During the enzymatic bioprocessing, most of the phenolics released during the hydrolysis, leached out into the liquid portion rather than retaining them within the millet grain, resulting in overall loss of dietary antioxidant. The present study establishes the most effective strategy to enrich the finger millet with phenolic antioxidants.

Research paper thumbnail of Optimiranje ekstrakcije polifenola iz okare

Food Technology and Biotechnology, Aug 10, 2011

Sažetak Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti... more Sažetak Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti održivi, ekološki postupak izdvajanja maksimalne količine polifenola. Da bi se ispitao utjecaj temperature, udjela otapala i vremena inkubacije na ekstrakciju polifenola, upotrijebljen je Box-Behnken dizajn metodologije odzivnih površina uz pomoć softvera MINITAB 15. Upotrijebljen je aceton kao otapalo za ekstrakciju fenolnih spojeva, te su ispitana svojstva polifenola, i to: sposobnost uklanjanja DPPH radikala, reducirajuća snaga i aktivnost ...