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Papers by steven arianto

Research paper thumbnail of Analisis Kadar Mirisetin Pada Ekstrak Daun Myrica Javanica Reinw. Ex Bl. Dengan Metode HPLC

Binawan Student Journal, Dec 30, 2023

Research paper thumbnail of Epigenetik Pada Sindrom Ovarium Polikistik: Gangguan Proses Folikulogenesis Yang Melibatkan Gen Reseptor Androgen, Reseptor Follicle-Stimulating Hormone Dan Reseptor Luteinizing Hormone

Indonesian Journal of Health Science, Feb 7, 2024

Research paper thumbnail of Pelatihan Verifikasi Jenis Kelamin Menggunakan Metode Barr Body dan Drum Stick

Masalah penentuan jenis kelamin dan kelainan kromosom seks pada manusia merupakah salah satu masa... more Masalah penentuan jenis kelamin dan kelainan kromosom seks pada manusia merupakah salah satu masalah dalam bidang kesehatan yang kerap terjadi. Akibat cukup tabunya masalah mengenai jenis kelamin, para ilmuwan mencoba mengembangkan metode-metode molekuler untuk mengatasi masalah tersebut. Salah satu metode molekuler untuk menganalisis jenis kelamin dan kelainan kromosom seks manusia adalah dengan metode Barr body dan drum stick. Metode ini masih cukup jarang dilakukan di Indonesia. Oleh karena itu pengabdian masyarakat kali ini mengambil tema pelatihan verifikasi jenis kelamin menggunakan metode Barr body dan drum stick pada mahasiswa dan mahasiswi dari institusi di bidang kesehatan. Kegiatan pengabdian masyarakat ini dilaksanakan pada tanggal 11 Maret 2023 di Teaching Laboratorium 2 Politeknik Kesehatan Hermina, Jakarta Timur. Jumlah peserta sebanyak 15 orang mahasiswa dan mahasiswi dari program studi Teknologi Laboratorium Medik di institusi daerah Jakarta dan Banten. Sebelum diberikan penyuluhan materi, para peserta diberikan kuis untuk melihat pemahaman awal peserta terhadap metode Barr body dan drum stick. Kegiatan yang dilakukan berupa workshop. Setelah diberikan penyuluhan materi, peserta langsung membuat preparat serta menganalisis Barr body dan drum stick. Hasil analisis Barr body dan drum stick tidak ditemukan adanya penyakit kromosomal seks pada peserta. Pada akhir kegiatan, peserta diberikan lagi kuis untuk melihat pemahaman peserta setelah workshop analisis Barr body dan drum stick. Berdasarkan hasil kuis, peserta lebih memahami tentang Barr body dan drum stick setelah kegiatan pengabdian kepada masyarakat.

Research paper thumbnail of Pemeriksaan Skrining Alternatif PAP SMEAR

Research paper thumbnail of Penggunaan Long-Lasting Insecticide-Treated Bednets (LLINS) Modifikasi Terbaru (Olyset Duo) Dalam Pengendalian Malaria

Indonesian Journal of Health Science

Malaria adalah penyakit yang disebabkan oleh parasit protozoa genus Plasmodium dan ditularkan mel... more Malaria adalah penyakit yang disebabkan oleh parasit protozoa genus Plasmodium dan ditularkan melalui gigitan nyamuk Anopheles betina. Ada lima jenis Plasmodium yang diketahui menyerang manusia yakni Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, dan yang terakhir ditemukan yaitu Plasmodium knowlesi. Pengendalian malaria dapat dilakukan dengan diagnosa dan pengobatan yang tepat, penggunaan kelambu tidur berinsektisida atau nama lainnya insecticide-treated net (ITN), dan penggunaan spray insektisida (indoor residual spraying with insecticides). Insecticide-treated net (ITN) konvensional memiliki efektivitas selama satu tahun, sedangkan ITN jenis LLINs (Long-lasting insecticide-treated bednets) memiliki efektivitas selama tiga tahun. Senyawa kimia yang terdapat pada LLINs yaitu pyrethroid. Sedangkan Olyset Duo adalah modifikasi terbaru dari LLINs (Long lasting Insecticide Nets) yang merupakan kombinasi dari dua bahan kimia yakni permentrin dan pyriprox...

Research paper thumbnail of Webinar Awam: Tata Laksana Pemeriksaan COVID-19 Dan Penanggulangan Demam Pada Anak DI Masa Pandemi COVID-19

Data on positive confirmed cases of covid-19 on February 2, 2021, there were 102,942,987, with de... more Data on positive confirmed cases of covid-19 on February 2, 2021, there were 102,942,987, with death cases reaching 2,232,233 in more than 170 countries. Based on the latest data from the Ministry of Health of The Republic of Indonesia, the number of confirmed cases in children aged 0-5 years is 2.8%, and aged 6-18 years is 9.8% of the total confirmed cases Covid-19 in Indonesia. The pandemic period caused the public to be worried if symptoms that led to Covid-19 appeared, one of which was fever in children. Therefore, these webinars aim to educate parents to stay calm when their child gets a fever during the pandemic and provide education about Covid-19 testing in children to understand and are not afraid to carry out these tests. The method used to provide education to the public is to hold webinars through the Zoom Teleconference application, broadcast live on the social media application Youtube and Instagram of Politeknik Kesehatan Hermina. The material provided in this activit...

Research paper thumbnail of Identification of novel variation in introns three and four of the iduronate-2-sulfatase gene in patients with mucopolysaccharidosis type II

Journal of Physics: Conference Series, Aug 1, 2018

To identify and analyze mutations that occur in introns of the iduronate-2-sulfatase (IDS) gene o... more To identify and analyze mutations that occur in introns of the iduronate-2-sulfatase (IDS) gene of patients with mucopolysaccharidosis type II (MPS II) in Indonesia. DNA samples from 6 patients with MPS II and 49 normal individuals were analyzed with direct sequencing of introns three and four. A novel variant was found at the site c.419-132del in intron three and three variants were found at c.507+26G>A, c.507+29G>A, and c.507+63T>G in intron four of the IDS gene. These variations occur in the non-splicing site of the IDS gene in patients with MPS II and normal individuals and do not affect the structure of the IDS gene. Alterations which occur in one site of intron three and three sites of intron four are new variations of the IDS gene found in Indonesian patients. This finding suggests that the IDS gene may show variation in other sites that could be associated with MPS II. These mutations can enrich the single nucleotide polymorphism database for the IDS gene globally. Further genotype studies should be performed to obtain a full profile of the IDS gene in patients with MPS II in Indonesia.

Research paper thumbnail of Protein analysis of exon 8 mutation in iduronate 2-sulfatase gene in mucopolysaccharidosis type II patients in Indonesia

THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019, 2019

Mucopolysaccharidosis II (MPS II, Hunter syndrome) is a rare, X-linked, recessive lysosomal stora... more Mucopolysaccharidosis II (MPS II, Hunter syndrome) is a rare, X-linked, recessive lysosomal storage disease caused by a deficiency of enzyme iduronate-2-sulfatase (I2S), encoded by IDS gene. I2S enzyme catalyzes the degradation of glycosaminoglycans (GAGs), such as dermatan sulfate (DS) and heparan sulfate (HS). Deficiency of I2S leads to the accumulation of these glycosaminoglycans in the tissues. Exon-specific analyses of IDS exon 8 has been analyzed from eight MPS II Indonesian patients at Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia. Identification of IDS exon 8 was performed using PCR and sequencing-based methods. One previously reported deletion mutation (c.1023delA) of exon 8 was identified amongst the patients, causing a frameshift in the corresponding amino acid sequence (p.Glu341AspfsTer19), observed in one patients. This mutation causes a reduction in the number of amino acids and changes in the structure of 3D proteins. There is no reduction and change in the active site of the protein, but the termination that occurs at the 359 th codon causes a reduction of two glycosylation sites. This study provides the first mutation analysis of exon 8 of IDS, and successfully identified mutations within the IDS gene that may be associated with MPS II. This supports the feasibility of early diagnosis and screening for MPS II in the future.

Research paper thumbnail of Peran SNP rs3735520 dan rs 12536657 Gen Hepatocyte Growth Factor dan MET+110703 Gen Reseptor cMET Sebagai Faktor Predisposisi Miopia

Hermina Health Sciences Journal, May 18, 2021

Research paper thumbnail of Mutation analysis of exon 8 of the iduronate-2-sulfatase gene in mucopolysaccharidosis type II patients in Indonesia

Journal of Natural Science, Biology and Medicine, 2019

Objective: Mucopolysaccharidosis II (MPS II), also known as Hunter syndrome, is a rare, recessive... more Objective: Mucopolysaccharidosis II (MPS II), also known as Hunter syndrome, is a rare, recessive, X-linked lysosomal storage disorder caused by a deficiency of lysosomal enzyme iduronate-2-sulfatase (IDS), encoded by IDS gene. I2S plays an important role in the lysosomal degradation of dermatan sulfate and heparan sulfate, with I2S deficiency leading to the accumulation of these glycosaminoglycans in the tissues. Materials and Methods: Exon-specific analyses of IDS exon 8 from eight Indonesian patients with MPS II from Cipto Mangunkusumo Hospital, Jakarta, Indonesia, were performed using polymerase chain reaction and sequencing-based methods. Results: Two novel mutations and a deletion variant of exon 8 were identified among the patients. A single-nucleotide deletion variant (c.1023delA), causing a frameshift in the corresponding amino acid sequence (p.Glu341AspfsTer19), was observed in all patients. In addition, a novel missense mutation (c.1033T>C) resulting in a tryptophan to...

Research paper thumbnail of Variant analysis for exon 2 and 5 of iduronate 2-sulfatase gene on mucopolysaccharidosis type II patients in Indonesia

Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is one of lysosomal storage disorder ca... more Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is one of lysosomal storage disorder caused by the presence of pathogenic variant in IDS gene. The variant can be found in various exon locations. This research aimed to identify the presence of disease-causing variant that may occurs at exon 2 and 5 of IDS gene on MPS II patient, especially in Indonesia. Based on the previous research that has been conducted in a number of countries, exon 2 and 5 are the exons with the most number of variations. Analysis was conducted on 7 MPS II patient of Indonesian origin and 50 normal individuals as control that consist of 25 male or 25 female individuals. Analysis was done by going through steps of DNA isolation, amplification by polymerase chain reaction (PCR), visualization by electrophoresis, and sequencing. Research result shows that IDS gene from whole samples were successfully analyzed. This study discovered an adenine base deletion c.708+72delA in intron 5 of one healthy individu...

Research paper thumbnail of The Effect of Honey PS (Pollen Substitute) on The Concentration of Triglyceride in Blood Plasma of Male White Rat (Rattus norvegicus L.) Sprague-Dawley Strain and Potential Benefits on Reducing Cardiovascular Risk Factor

Honey has potential benefits on reducing cardiovascular risk factors which is caused mainly by hi... more Honey has potential benefits on reducing cardiovascular risk factors which is caused mainly by high consumption of triglyceride. This study investigated the effect of honey PS (Pollen Substitute) on triglyceride concentration using 24 adult male Sprague-Dawley rats. Honey PS is produced by Asiatic honey bee, Apis cerana, which is fed on with soy flour, skim milk, and yeast, Candida hawaiiana. Twenty four male white rats Sprague-Dawley strain weighing between 180-200 grams. The rats were randomly assigned into 4 groups, consisting of group 1 (normal control group)-standard normal diet and water, group 2 (treatment control group)-high lipid diet and water, group 3-high lipid diet and 10% honey PS solution, and group 4-high lipid diet and 20% honey PS solution, six in ech group. High lipid diet contained 50% yolk, 40% chicken fat, and 10% of 65% sucrose solution. After 14 days, the rats were sacrificed and blood collected by plexus-retro-orbitalis after a 16 hour of fasting. Their sera...

Research paper thumbnail of Analisis Kadar Mirisetin Pada Ekstrak Daun Myrica Javanica Reinw. Ex Bl. Dengan Metode HPLC

Binawan Student Journal, Dec 30, 2023

Research paper thumbnail of Epigenetik Pada Sindrom Ovarium Polikistik: Gangguan Proses Folikulogenesis Yang Melibatkan Gen Reseptor Androgen, Reseptor Follicle-Stimulating Hormone Dan Reseptor Luteinizing Hormone

Indonesian Journal of Health Science, Feb 7, 2024

Research paper thumbnail of Pelatihan Verifikasi Jenis Kelamin Menggunakan Metode Barr Body dan Drum Stick

Masalah penentuan jenis kelamin dan kelainan kromosom seks pada manusia merupakah salah satu masa... more Masalah penentuan jenis kelamin dan kelainan kromosom seks pada manusia merupakah salah satu masalah dalam bidang kesehatan yang kerap terjadi. Akibat cukup tabunya masalah mengenai jenis kelamin, para ilmuwan mencoba mengembangkan metode-metode molekuler untuk mengatasi masalah tersebut. Salah satu metode molekuler untuk menganalisis jenis kelamin dan kelainan kromosom seks manusia adalah dengan metode Barr body dan drum stick. Metode ini masih cukup jarang dilakukan di Indonesia. Oleh karena itu pengabdian masyarakat kali ini mengambil tema pelatihan verifikasi jenis kelamin menggunakan metode Barr body dan drum stick pada mahasiswa dan mahasiswi dari institusi di bidang kesehatan. Kegiatan pengabdian masyarakat ini dilaksanakan pada tanggal 11 Maret 2023 di Teaching Laboratorium 2 Politeknik Kesehatan Hermina, Jakarta Timur. Jumlah peserta sebanyak 15 orang mahasiswa dan mahasiswi dari program studi Teknologi Laboratorium Medik di institusi daerah Jakarta dan Banten. Sebelum diberikan penyuluhan materi, para peserta diberikan kuis untuk melihat pemahaman awal peserta terhadap metode Barr body dan drum stick. Kegiatan yang dilakukan berupa workshop. Setelah diberikan penyuluhan materi, peserta langsung membuat preparat serta menganalisis Barr body dan drum stick. Hasil analisis Barr body dan drum stick tidak ditemukan adanya penyakit kromosomal seks pada peserta. Pada akhir kegiatan, peserta diberikan lagi kuis untuk melihat pemahaman peserta setelah workshop analisis Barr body dan drum stick. Berdasarkan hasil kuis, peserta lebih memahami tentang Barr body dan drum stick setelah kegiatan pengabdian kepada masyarakat.

Research paper thumbnail of Pemeriksaan Skrining Alternatif PAP SMEAR

Research paper thumbnail of Penggunaan Long-Lasting Insecticide-Treated Bednets (LLINS) Modifikasi Terbaru (Olyset Duo) Dalam Pengendalian Malaria

Indonesian Journal of Health Science

Malaria adalah penyakit yang disebabkan oleh parasit protozoa genus Plasmodium dan ditularkan mel... more Malaria adalah penyakit yang disebabkan oleh parasit protozoa genus Plasmodium dan ditularkan melalui gigitan nyamuk Anopheles betina. Ada lima jenis Plasmodium yang diketahui menyerang manusia yakni Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, dan yang terakhir ditemukan yaitu Plasmodium knowlesi. Pengendalian malaria dapat dilakukan dengan diagnosa dan pengobatan yang tepat, penggunaan kelambu tidur berinsektisida atau nama lainnya insecticide-treated net (ITN), dan penggunaan spray insektisida (indoor residual spraying with insecticides). Insecticide-treated net (ITN) konvensional memiliki efektivitas selama satu tahun, sedangkan ITN jenis LLINs (Long-lasting insecticide-treated bednets) memiliki efektivitas selama tiga tahun. Senyawa kimia yang terdapat pada LLINs yaitu pyrethroid. Sedangkan Olyset Duo adalah modifikasi terbaru dari LLINs (Long lasting Insecticide Nets) yang merupakan kombinasi dari dua bahan kimia yakni permentrin dan pyriprox...

Research paper thumbnail of Webinar Awam: Tata Laksana Pemeriksaan COVID-19 Dan Penanggulangan Demam Pada Anak DI Masa Pandemi COVID-19

Data on positive confirmed cases of covid-19 on February 2, 2021, there were 102,942,987, with de... more Data on positive confirmed cases of covid-19 on February 2, 2021, there were 102,942,987, with death cases reaching 2,232,233 in more than 170 countries. Based on the latest data from the Ministry of Health of The Republic of Indonesia, the number of confirmed cases in children aged 0-5 years is 2.8%, and aged 6-18 years is 9.8% of the total confirmed cases Covid-19 in Indonesia. The pandemic period caused the public to be worried if symptoms that led to Covid-19 appeared, one of which was fever in children. Therefore, these webinars aim to educate parents to stay calm when their child gets a fever during the pandemic and provide education about Covid-19 testing in children to understand and are not afraid to carry out these tests. The method used to provide education to the public is to hold webinars through the Zoom Teleconference application, broadcast live on the social media application Youtube and Instagram of Politeknik Kesehatan Hermina. The material provided in this activit...

Research paper thumbnail of Identification of novel variation in introns three and four of the iduronate-2-sulfatase gene in patients with mucopolysaccharidosis type II

Journal of Physics: Conference Series, Aug 1, 2018

To identify and analyze mutations that occur in introns of the iduronate-2-sulfatase (IDS) gene o... more To identify and analyze mutations that occur in introns of the iduronate-2-sulfatase (IDS) gene of patients with mucopolysaccharidosis type II (MPS II) in Indonesia. DNA samples from 6 patients with MPS II and 49 normal individuals were analyzed with direct sequencing of introns three and four. A novel variant was found at the site c.419-132del in intron three and three variants were found at c.507+26G>A, c.507+29G>A, and c.507+63T>G in intron four of the IDS gene. These variations occur in the non-splicing site of the IDS gene in patients with MPS II and normal individuals and do not affect the structure of the IDS gene. Alterations which occur in one site of intron three and three sites of intron four are new variations of the IDS gene found in Indonesian patients. This finding suggests that the IDS gene may show variation in other sites that could be associated with MPS II. These mutations can enrich the single nucleotide polymorphism database for the IDS gene globally. Further genotype studies should be performed to obtain a full profile of the IDS gene in patients with MPS II in Indonesia.

Research paper thumbnail of Protein analysis of exon 8 mutation in iduronate 2-sulfatase gene in mucopolysaccharidosis type II patients in Indonesia

THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019, 2019

Mucopolysaccharidosis II (MPS II, Hunter syndrome) is a rare, X-linked, recessive lysosomal stora... more Mucopolysaccharidosis II (MPS II, Hunter syndrome) is a rare, X-linked, recessive lysosomal storage disease caused by a deficiency of enzyme iduronate-2-sulfatase (I2S), encoded by IDS gene. I2S enzyme catalyzes the degradation of glycosaminoglycans (GAGs), such as dermatan sulfate (DS) and heparan sulfate (HS). Deficiency of I2S leads to the accumulation of these glycosaminoglycans in the tissues. Exon-specific analyses of IDS exon 8 has been analyzed from eight MPS II Indonesian patients at Cipto Mangunkusumo National Referral Hospital, Jakarta, Indonesia. Identification of IDS exon 8 was performed using PCR and sequencing-based methods. One previously reported deletion mutation (c.1023delA) of exon 8 was identified amongst the patients, causing a frameshift in the corresponding amino acid sequence (p.Glu341AspfsTer19), observed in one patients. This mutation causes a reduction in the number of amino acids and changes in the structure of 3D proteins. There is no reduction and change in the active site of the protein, but the termination that occurs at the 359 th codon causes a reduction of two glycosylation sites. This study provides the first mutation analysis of exon 8 of IDS, and successfully identified mutations within the IDS gene that may be associated with MPS II. This supports the feasibility of early diagnosis and screening for MPS II in the future.

Research paper thumbnail of Peran SNP rs3735520 dan rs 12536657 Gen Hepatocyte Growth Factor dan MET+110703 Gen Reseptor cMET Sebagai Faktor Predisposisi Miopia

Hermina Health Sciences Journal, May 18, 2021

Research paper thumbnail of Mutation analysis of exon 8 of the iduronate-2-sulfatase gene in mucopolysaccharidosis type II patients in Indonesia

Journal of Natural Science, Biology and Medicine, 2019

Objective: Mucopolysaccharidosis II (MPS II), also known as Hunter syndrome, is a rare, recessive... more Objective: Mucopolysaccharidosis II (MPS II), also known as Hunter syndrome, is a rare, recessive, X-linked lysosomal storage disorder caused by a deficiency of lysosomal enzyme iduronate-2-sulfatase (IDS), encoded by IDS gene. I2S plays an important role in the lysosomal degradation of dermatan sulfate and heparan sulfate, with I2S deficiency leading to the accumulation of these glycosaminoglycans in the tissues. Materials and Methods: Exon-specific analyses of IDS exon 8 from eight Indonesian patients with MPS II from Cipto Mangunkusumo Hospital, Jakarta, Indonesia, were performed using polymerase chain reaction and sequencing-based methods. Results: Two novel mutations and a deletion variant of exon 8 were identified among the patients. A single-nucleotide deletion variant (c.1023delA), causing a frameshift in the corresponding amino acid sequence (p.Glu341AspfsTer19), was observed in all patients. In addition, a novel missense mutation (c.1033T>C) resulting in a tryptophan to...

Research paper thumbnail of Variant analysis for exon 2 and 5 of iduronate 2-sulfatase gene on mucopolysaccharidosis type II patients in Indonesia

Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is one of lysosomal storage disorder ca... more Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is one of lysosomal storage disorder caused by the presence of pathogenic variant in IDS gene. The variant can be found in various exon locations. This research aimed to identify the presence of disease-causing variant that may occurs at exon 2 and 5 of IDS gene on MPS II patient, especially in Indonesia. Based on the previous research that has been conducted in a number of countries, exon 2 and 5 are the exons with the most number of variations. Analysis was conducted on 7 MPS II patient of Indonesian origin and 50 normal individuals as control that consist of 25 male or 25 female individuals. Analysis was done by going through steps of DNA isolation, amplification by polymerase chain reaction (PCR), visualization by electrophoresis, and sequencing. Research result shows that IDS gene from whole samples were successfully analyzed. This study discovered an adenine base deletion c.708+72delA in intron 5 of one healthy individu...

Research paper thumbnail of The Effect of Honey PS (Pollen Substitute) on The Concentration of Triglyceride in Blood Plasma of Male White Rat (Rattus norvegicus L.) Sprague-Dawley Strain and Potential Benefits on Reducing Cardiovascular Risk Factor

Honey has potential benefits on reducing cardiovascular risk factors which is caused mainly by hi... more Honey has potential benefits on reducing cardiovascular risk factors which is caused mainly by high consumption of triglyceride. This study investigated the effect of honey PS (Pollen Substitute) on triglyceride concentration using 24 adult male Sprague-Dawley rats. Honey PS is produced by Asiatic honey bee, Apis cerana, which is fed on with soy flour, skim milk, and yeast, Candida hawaiiana. Twenty four male white rats Sprague-Dawley strain weighing between 180-200 grams. The rats were randomly assigned into 4 groups, consisting of group 1 (normal control group)-standard normal diet and water, group 2 (treatment control group)-high lipid diet and water, group 3-high lipid diet and 10% honey PS solution, and group 4-high lipid diet and 20% honey PS solution, six in ech group. High lipid diet contained 50% yolk, 40% chicken fat, and 10% of 65% sucrose solution. After 14 days, the rats were sacrificed and blood collected by plexus-retro-orbitalis after a 16 hour of fasting. Their sera...