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Papers by nuray ari

One of the main goals of treatment of diabetes mellitus is to prevent its complications. Oxidativ... more One of the main goals of treatment of diabetes mellitus is to prevent its complications. Oxidative stress is universal in diabetes, being ultimately involved with the development complications. As a result of hyperglycemia, reactive oxygen/nitrogen species are produced in various tissues that leads to tissue damage with lipid peroxidation and protein oxidation, along with disruption in cellular homeostasis and accumulation of damaged molecules. Hence, supplementation with antioxidant compounds may offer some protection against diabetic complications. The pleiotropic effects of statins, including antioxidant and anti-inflammatory properties, represent an area of great interest in prevention and therapy of cardiovascular and neurological disorders. Using biomarkers of oxidative stress, in this study we examined the effect of non cholesterol lowering dose, long term fluvastatin treatment on oxidative stress in streptozotocin-diabetic rats. Experiments were conducted in 24 Wistar adult male rats. Diabetic and non-diabetic rats were treated orally for 6 months with fluvastatin (2 mg/kg/day, p.o) starting one week after streptozotocin injection (55 mg/kg, i.p.), (preventive study). In brain, heart, liver, pancreas and kidney homogenates malondialdehyde, lipid hydroperoxide, protein carbonyl content, advanced oxidation protein products, 3-nitrotyrosine levels and superoxide dismutase, catalase activities were measured. Hyperglycemia and dyslipidemia in diabetic groups remained unchanged after fluvastatin treatment. The drug act as antioxidant in the tissues. Hence, antioxidant property of fluvastatin, independent of cholesterol lowering effect, may play a role in prevention of diabetic complications. Clinical relevance of this effect of fluvastatin seems worthy of further studies.

Universal Journal of Pharmaceutical Research, 2021

Statins, HMG CoA inhibitors, are potent hypolipidemic drugs.They are used for the prevention of c... more Statins, HMG CoA inhibitors, are potent hypolipidemic drugs.They are used for the prevention of cardiovascular diseases and some of the most commonly used drugs worldwide. Long term statin therapy can cause a modest increase in new-onset diabetes risk, and there is great interest in the mechanisms for this adverse effect. Main proposed mechanisms are increased insulin resistance and some defects in insulin secretion. Many factors can affect the risk including pre-existing diabetic risk, older age and potency of statin. But clearly, the benefits of these drugs in preventing cardiovascular disease outweigh the potential risk of diabetes.The aim of this review is to give underlying pathomechanisms and clinical relevance of diabetogenic effect of statins. Peer Review History: Received: 18 May 2021; Revised: 24 June; Accepted: 29 June, Available online: 15 July 2021 Academic Editor: Dr. Ali Abdullah Al-yahawi, Al-Razi university, Department of Pharmacy, Yemen, alyahaw...

IJEB Vol.59(06) [June 2021], Jun 1, 2021

In diabetes, one of the most important causes of morbidity and mortality is vasculopathy. Though ... more In diabetes, one of the most important causes of morbidity and mortality is vasculopathy. Though flaxseed (FXS) is known for improving cardiovascular health, only limited studies are available on FXS concerning diabetic vascular reactivity. Hence, in this study, we studied vascular reactivity changes after FXS treatment on streptozotocin (STZ)-induced diabetic rat aortae. Female Wistar rats were divided into following four groups: control (C), FXS treated (CT), diabetic (D), and FXS treated diabetic (DT) groups. FXS (0,714 g/kg/day; orally) was started after one week of STZ injection and was given for 12 weeks, phenylephrine (Phe)-induced contractions were obtained on isolated aortic rings in the presence of indomethacin, L-NAME and superoxide dismutase (SOD), individually. Phe-responses were increased significantly in D group and completely improved after FXS intake, whereas FXS increased vascular reactivity to Phe in C group. Indomethacin incubation significantly attenuated Phe-induced contractions in all groups of aorta, particularly in D group. L-NAME incubation significantly increased Phe responses in all groups except D group. SOD incubation decreased the contractions efficiently in D group. The decreament was much lower in DT compared to D group, but reverse effects were observed in CT group. Our findings suggest FXS may provide beneficial effects on diabetes-induced vascular reactivity changes through NO and prostaglandin dependent pathways, but in healty condition FXS may have adverse effect probably via pro-oxidant activity.

The aim of this study was to investigate whether atorvastatin ameliorates diabetes-induced cardio... more The aim of this study was to investigate whether atorvastatin ameliorates diabetes-induced cardiomyocyte dysfunction, independently of cholesterol-lowering effect. Streptozotocin-induced diabetic mice..

Journal of Pharmacy and Pharmacology, 2006

Vascular reactivity was investigated in endothelium-denuded human internal mammary artery (IMA) r... more Vascular reactivity was investigated in endothelium-denuded human internal mammary artery (IMA) rings from type 2 diabetic patients. It was also investigated whether insulin sensitizer thiazolidinedione drugs, pioglitazone and rosiglitazone, can directly affect the reactivity of IMA. Using organ bath techniques, cumulative concentration-response curves to phenylephrine (PE), KCl, cromakalim (CRO) and sodium nitroprusside (SNP) were constructed in diabetic and non-diabetic IMA rings. Means of maximal responses (% Emax) and pEC50 values (sensitivity) were compared. Emax values and the sensitivity to PE and KCl were increased while KATP-channel-mediated relaxations were reduced significantly in diabetic rings compared with non-diabetic rings (n = 5–12, P < 0.05). No changes were observed for SNP responses (n = 5, P > 0.05). Incubations with pioglitazone (1 and 10 μM) and rosiglitazone (1 and 20 μM), for 30 min, did not affect KATP-channel-mediated relaxations (n = 5 each, P > ...

Turkiye Klinikleri Tip Bilimleri Dergisi, 2008

Turkish Journal of Pharmaceutical Sciences, 2010

In this study, effect of solvent system on in vitro granisetron HCl release from injectable in si... more In this study, effect of solvent system on in vitro granisetron HCl release from injectable in situ forming implants were investigated also by means of gamma irradiation sterilization. Implant formulations contain 56% solvent, 38% polymer and 6% drug. For the preparation of the formulations, hydrophobic benzyl benzoate and moderately hydrophobic prophylene carbonate were used as solvents while medium molecular weight poly(DL-lactide-co-glycolide) (Resomer RG 503H) was used as polymer and the formulation containing the solvents in 1:1 combination showed low initial burst and acceptable regular release of drug for 21 days. It was determined that drug release from this formulation was increased by application of gamma irradiation and the sterilization effect were investigated on solidified implant system by the morphological analysis, which used as descriptive for in vitro release behavior of drug. In vivo performance of the mentioned sterile formulation was investigated on rabbits and...

A positive correlation has been established between increased oxidative stress and cardiovascular... more A positive correlation has been established between increased oxidative stress and cardiovascular diseases in diabetes mellitus. We evaluated the effects of single or combined treatments with vitamin A (retinol acetate, 30 mg/kg/day, for 12-weeks) and insulin (8-10 IU/rat/day for the final 6-week) on vasomotor activity, oxidative stress and retinol metabolism in 12-week streptozotocin diabetic rats. The vasomotor activity was determined by measuring in vitro responsiveness of aorta rings to phenylephrine (PE) and acetylcholine (ACh) in the absence or in the presence of hydrogen peroxide (H 2 O 2 ). Preincubation with H 2 O 2 (10 µM) produced a significant decrease in PE (1 mM)-induced contraction in untreated-diabetic but not in control rats. Single treatment with insulin counteracted this effect of H 2 O 2 and also reversed the increased contractile response of diabetic aorta to PE, while vitamin A was found to be ineffective. H 2 O 2 (10 µM) also inhibited ACh (1 mM)-stimulated en...

Neuro endocrinology letters, 2010

OBJECTIVES Recent basic research and clinical data have provided new insights into the role of gl... more OBJECTIVES Recent basic research and clinical data have provided new insights into the role of glycoxidative and nitrosative stresses (both oxidative stress) in diabetic complications, such as diabetic nephropathy, suggesting a different and innovative approach to antioxidant therapy. In streptozotocin-induced diabetic rat kidney, the present study investigated the effects of the synthetic pyridoindole antioxidant stobadine (STB) on renal total antioxidant potential (AOP) and protein oxidation parameters such as protein carbonyl content (PCC), advanced oxidation protein products (AOPPs) and nitrotyrosine (NT), a marker specific for protein modification by peroxynitrite. MATERIALS AND METHODS Wistar Albino rats were divided into two groups: normal and streptozotocin-induced diabetic rats. Each group of the animals was further divided into two groups: untreated and treated with stobadine (24.7 mg/kg) during 16 weeks daily by oral gavage. RESULTS The renal tissue AOP and the levels of ...

ACTA Pharmaceutica Sciencia, 2016

Diabetes mellitus, a metabolic disorder, is characterized by absolute or relative deficiencies in... more Diabetes mellitus, a metabolic disorder, is characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycemia. The prevalence of diabetes is increasing worldwide, especially in developing countries. The diabetes treatment has higher costs, limited efficacy and side effects. As a result of these factors, patients often have used alternative forms of therapy such as herbal medicines. Plants often contain various amounts of phenolics, flavonoids and tannins and most of the studies are focused on the antidiabetic effects of these phytochemicals due to their antioxidant properties. In this review, the role of oxidative stress on diabetes and the effects of different phytochemicals (limonene, sinnamic acid and ursolic acid) to diabetes mellitus therapy will be discussed.

Interdisciplinary Toxicology

The pentose phosphate pathway and glutathione-associated metabolism are the main antioxidant cell... more The pentose phosphate pathway and glutathione-associated metabolism are the main antioxidant cellular defense systems. This study investigated the effects of the powerful antioxidant SMe1EC2 (2-ethoxycarbonyl-8-methoxy-2,3,4,4a,5,9b-hexahydro-1H-pyrido[4,3-b] indolinium dichloride) on pentose phosphate pathway (PPP) and glutathione-dependent enzyme activities in aged diabetic and aged matched control rats. Diabetes was induced by streptozotocin injection in rats aged 13-15 months. Diabetic and control rats were divided into two subgroups, one untreated and one treated with SMe1EC2 (10 mg/kg/day, orally) for 4 months. SMe1EC2 ameliorated body weight loss, but not hyperglycemia of aged diabetic rats. Diabetes resulted in decreased glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD) and glutathione-S-transferase (GST), yet in unchanged glutathione reductase (GR) in the liver of aged diabetic rats. In the liver of the aged control rats, SMe1EC2 did not affe...

The Turkish Journal of Pharmaceutical Sciences

Amaç: Metabolik ve kronik bir hastalık olan diyabetin birçok ülkede önemli sağlık sorunu olma dur... more Amaç: Metabolik ve kronik bir hastalık olan diyabetin birçok ülkede önemli sağlık sorunu olma durumu artış göstermektedir. Diyabetin artmış serbest radikallerin üretimi ve azalmış antioksidan kapasiteyle ilişkili olduğu iddia edilmektedir. Diyabette oluşan oksiatif stres dokularda DNA hasarına neden olabilir. Antioksidan, antikanser, antiülser özellikler gibi sağlığa yararlı etkileri nedeniyle geleneksel Çin halk tıbbında yaygın olarak kullanılan ursolik asit çok iyi tanımlanmış pentasiklik triterpendir. Bu çalışmanın amacı, streptozotosin ile diyabet oluşturulmuş Wistar albino sıçanların böbreklerine ursolik asidin etkisini değerlendirmektir. Gereç ve Yöntemler: Bu amaçla, böbrek hücrelerindeki DNA hasarı alkali comet yöntemiyle araştırılmıştır. KAT, SOD, GR ve GSH-Px enzim aktiviteleri ve toplam GSH ve MDA düzeyleri gibi oksidatif stres parametreleri de ölçülmüştür. Bulgular: Ursolik asit tedavisinin, DNA hasarını, GR enzim aktivitelerini ve MDA seviyelerini önemli ölçüde azalttığı ve diyabetik sıçanlarda GSH düzeylerini ve CAT, SOD ve GSH-Px enzim aktivitelerini önemli ölçüde artırdığı bulundu. Sonuç: Sonuçlarımıza göre, ursolik asidin diyabetin neden olduğu böbrek hasarında yararlı olabileceği görülmüştür. Anahtar kelimeler: Diyabet, DNA hasarı, oksidatif stres, böbrek Objectives: Diabetes, a heteregenous metabolic and chronic disease, is a growing health problem in most countries. It has been claimed that diabetes is associated with the increased formation of free radicals and decreased in antioxidant potential. Oxidative stress formed in diabetes may cause DNA damage in the tissues. Ursolic acid, a well-known pentacylic triterpene, is commonly used in traditional Chinese medicine due to its beneficial health effects such as antioxidant, anticancer, and antiulcer properties. The aim of this study was to investigate the effects of ursolic acid in the kidneys of Wistar albino rats with streptozotocin-induced diabetes. Materials and Methods: DNA damage was evaluated in the kidney cells of rats using alkaline comet assays. Oxidative stress parameters such as CAT, SOD, GR, and GSH-Px enzyme activities and total GSH and MDA levels were also evaluated. Results: Ursolic acid treatment was found to significantly decrease DNA damage, GR enzyme activities, and MDA levels, and significantly increase GSH levels and CAT, SOD and GSH-Px enzyme activities in diabetic rats. Conclusion: According to our results, it seems that ursolic acid may be beneficial against diabetes-induced renal damage.

Turkish journal of medical sciences, Jan 23, 2018

Background/aim: Diabetes mellitus (DM) is a major health problem worldwide. Cinnamic acid (CA) an... more Background/aim: Diabetes mellitus (DM) is a major health problem worldwide. Cinnamic acid (CA) and its derivatives are synthesized in plants and increasing attention has been given to them in recent years due to the high number of beneficial health properties attributed to their consumption. The aim of this study was to investigate the effects of CA on streptozotocin-induced diabetes in Wistar albino rats. Materials and methods: DNA damage was evaluated in the blood, liver, and kidney cells of rats by the alkaline comet assay. Oxidative stress parameters such as catalase, superoxide dismutase, glutathione reductase, glutathione-S-transferase, and glutathione peroxidase activities and 8-hydroxy-2-deoxyguanosine, total glutathione, and malondialdehyde levels; biochemical parameters including insulin, total bilirubin, and BCA protein levels; hepatic enzyme levels such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase; and lipi...

Food and Chemical Toxicology

Turkiye Klinikleri Journal of Pharmacy Sciences, 2017

Turkiye Klinikleri Journal of Internal Medical Sciences, 2005

Turkiye Klinikleri Journal of Cosmetic Dermatology Special Topics, 2008

Turkiye Klinikleri Journal of Internal Medical Sciences, 2005

Turkiye Klinikleri Journal of Medical Sciences, 2008

One of the main goals of treatment of diabetes mellitus is to prevent its complications. Oxidativ... more One of the main goals of treatment of diabetes mellitus is to prevent its complications. Oxidative stress is universal in diabetes, being ultimately involved with the development complications. As a result of hyperglycemia, reactive oxygen/nitrogen species are produced in various tissues that leads to tissue damage with lipid peroxidation and protein oxidation, along with disruption in cellular homeostasis and accumulation of damaged molecules. Hence, supplementation with antioxidant compounds may offer some protection against diabetic complications. The pleiotropic effects of statins, including antioxidant and anti-inflammatory properties, represent an area of great interest in prevention and therapy of cardiovascular and neurological disorders. Using biomarkers of oxidative stress, in this study we examined the effect of non cholesterol lowering dose, long term fluvastatin treatment on oxidative stress in streptozotocin-diabetic rats. Experiments were conducted in 24 Wistar adult male rats. Diabetic and non-diabetic rats were treated orally for 6 months with fluvastatin (2 mg/kg/day, p.o) starting one week after streptozotocin injection (55 mg/kg, i.p.), (preventive study). In brain, heart, liver, pancreas and kidney homogenates malondialdehyde, lipid hydroperoxide, protein carbonyl content, advanced oxidation protein products, 3-nitrotyrosine levels and superoxide dismutase, catalase activities were measured. Hyperglycemia and dyslipidemia in diabetic groups remained unchanged after fluvastatin treatment. The drug act as antioxidant in the tissues. Hence, antioxidant property of fluvastatin, independent of cholesterol lowering effect, may play a role in prevention of diabetic complications. Clinical relevance of this effect of fluvastatin seems worthy of further studies.

Universal Journal of Pharmaceutical Research, 2021

Statins, HMG CoA inhibitors, are potent hypolipidemic drugs.They are used for the prevention of c... more Statins, HMG CoA inhibitors, are potent hypolipidemic drugs.They are used for the prevention of cardiovascular diseases and some of the most commonly used drugs worldwide. Long term statin therapy can cause a modest increase in new-onset diabetes risk, and there is great interest in the mechanisms for this adverse effect. Main proposed mechanisms are increased insulin resistance and some defects in insulin secretion. Many factors can affect the risk including pre-existing diabetic risk, older age and potency of statin. But clearly, the benefits of these drugs in preventing cardiovascular disease outweigh the potential risk of diabetes.The aim of this review is to give underlying pathomechanisms and clinical relevance of diabetogenic effect of statins. Peer Review History: Received: 18 May 2021; Revised: 24 June; Accepted: 29 June, Available online: 15 July 2021 Academic Editor: Dr. Ali Abdullah Al-yahawi, Al-Razi university, Department of Pharmacy, Yemen, alyahaw...

IJEB Vol.59(06) [June 2021], Jun 1, 2021

In diabetes, one of the most important causes of morbidity and mortality is vasculopathy. Though ... more In diabetes, one of the most important causes of morbidity and mortality is vasculopathy. Though flaxseed (FXS) is known for improving cardiovascular health, only limited studies are available on FXS concerning diabetic vascular reactivity. Hence, in this study, we studied vascular reactivity changes after FXS treatment on streptozotocin (STZ)-induced diabetic rat aortae. Female Wistar rats were divided into following four groups: control (C), FXS treated (CT), diabetic (D), and FXS treated diabetic (DT) groups. FXS (0,714 g/kg/day; orally) was started after one week of STZ injection and was given for 12 weeks, phenylephrine (Phe)-induced contractions were obtained on isolated aortic rings in the presence of indomethacin, L-NAME and superoxide dismutase (SOD), individually. Phe-responses were increased significantly in D group and completely improved after FXS intake, whereas FXS increased vascular reactivity to Phe in C group. Indomethacin incubation significantly attenuated Phe-induced contractions in all groups of aorta, particularly in D group. L-NAME incubation significantly increased Phe responses in all groups except D group. SOD incubation decreased the contractions efficiently in D group. The decreament was much lower in DT compared to D group, but reverse effects were observed in CT group. Our findings suggest FXS may provide beneficial effects on diabetes-induced vascular reactivity changes through NO and prostaglandin dependent pathways, but in healty condition FXS may have adverse effect probably via pro-oxidant activity.

The aim of this study was to investigate whether atorvastatin ameliorates diabetes-induced cardio... more The aim of this study was to investigate whether atorvastatin ameliorates diabetes-induced cardiomyocyte dysfunction, independently of cholesterol-lowering effect. Streptozotocin-induced diabetic mice..

Journal of Pharmacy and Pharmacology, 2006

Vascular reactivity was investigated in endothelium-denuded human internal mammary artery (IMA) r... more Vascular reactivity was investigated in endothelium-denuded human internal mammary artery (IMA) rings from type 2 diabetic patients. It was also investigated whether insulin sensitizer thiazolidinedione drugs, pioglitazone and rosiglitazone, can directly affect the reactivity of IMA. Using organ bath techniques, cumulative concentration-response curves to phenylephrine (PE), KCl, cromakalim (CRO) and sodium nitroprusside (SNP) were constructed in diabetic and non-diabetic IMA rings. Means of maximal responses (% Emax) and pEC50 values (sensitivity) were compared. Emax values and the sensitivity to PE and KCl were increased while KATP-channel-mediated relaxations were reduced significantly in diabetic rings compared with non-diabetic rings (n = 5–12, P < 0.05). No changes were observed for SNP responses (n = 5, P > 0.05). Incubations with pioglitazone (1 and 10 μM) and rosiglitazone (1 and 20 μM), for 30 min, did not affect KATP-channel-mediated relaxations (n = 5 each, P > ...

Turkiye Klinikleri Tip Bilimleri Dergisi, 2008

Turkish Journal of Pharmaceutical Sciences, 2010

In this study, effect of solvent system on in vitro granisetron HCl release from injectable in si... more In this study, effect of solvent system on in vitro granisetron HCl release from injectable in situ forming implants were investigated also by means of gamma irradiation sterilization. Implant formulations contain 56% solvent, 38% polymer and 6% drug. For the preparation of the formulations, hydrophobic benzyl benzoate and moderately hydrophobic prophylene carbonate were used as solvents while medium molecular weight poly(DL-lactide-co-glycolide) (Resomer RG 503H) was used as polymer and the formulation containing the solvents in 1:1 combination showed low initial burst and acceptable regular release of drug for 21 days. It was determined that drug release from this formulation was increased by application of gamma irradiation and the sterilization effect were investigated on solidified implant system by the morphological analysis, which used as descriptive for in vitro release behavior of drug. In vivo performance of the mentioned sterile formulation was investigated on rabbits and...

A positive correlation has been established between increased oxidative stress and cardiovascular... more A positive correlation has been established between increased oxidative stress and cardiovascular diseases in diabetes mellitus. We evaluated the effects of single or combined treatments with vitamin A (retinol acetate, 30 mg/kg/day, for 12-weeks) and insulin (8-10 IU/rat/day for the final 6-week) on vasomotor activity, oxidative stress and retinol metabolism in 12-week streptozotocin diabetic rats. The vasomotor activity was determined by measuring in vitro responsiveness of aorta rings to phenylephrine (PE) and acetylcholine (ACh) in the absence or in the presence of hydrogen peroxide (H 2 O 2 ). Preincubation with H 2 O 2 (10 µM) produced a significant decrease in PE (1 mM)-induced contraction in untreated-diabetic but not in control rats. Single treatment with insulin counteracted this effect of H 2 O 2 and also reversed the increased contractile response of diabetic aorta to PE, while vitamin A was found to be ineffective. H 2 O 2 (10 µM) also inhibited ACh (1 mM)-stimulated en...

Neuro endocrinology letters, 2010

OBJECTIVES Recent basic research and clinical data have provided new insights into the role of gl... more OBJECTIVES Recent basic research and clinical data have provided new insights into the role of glycoxidative and nitrosative stresses (both oxidative stress) in diabetic complications, such as diabetic nephropathy, suggesting a different and innovative approach to antioxidant therapy. In streptozotocin-induced diabetic rat kidney, the present study investigated the effects of the synthetic pyridoindole antioxidant stobadine (STB) on renal total antioxidant potential (AOP) and protein oxidation parameters such as protein carbonyl content (PCC), advanced oxidation protein products (AOPPs) and nitrotyrosine (NT), a marker specific for protein modification by peroxynitrite. MATERIALS AND METHODS Wistar Albino rats were divided into two groups: normal and streptozotocin-induced diabetic rats. Each group of the animals was further divided into two groups: untreated and treated with stobadine (24.7 mg/kg) during 16 weeks daily by oral gavage. RESULTS The renal tissue AOP and the levels of ...

ACTA Pharmaceutica Sciencia, 2016

Diabetes mellitus, a metabolic disorder, is characterized by absolute or relative deficiencies in... more Diabetes mellitus, a metabolic disorder, is characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycemia. The prevalence of diabetes is increasing worldwide, especially in developing countries. The diabetes treatment has higher costs, limited efficacy and side effects. As a result of these factors, patients often have used alternative forms of therapy such as herbal medicines. Plants often contain various amounts of phenolics, flavonoids and tannins and most of the studies are focused on the antidiabetic effects of these phytochemicals due to their antioxidant properties. In this review, the role of oxidative stress on diabetes and the effects of different phytochemicals (limonene, sinnamic acid and ursolic acid) to diabetes mellitus therapy will be discussed.

Interdisciplinary Toxicology

The pentose phosphate pathway and glutathione-associated metabolism are the main antioxidant cell... more The pentose phosphate pathway and glutathione-associated metabolism are the main antioxidant cellular defense systems. This study investigated the effects of the powerful antioxidant SMe1EC2 (2-ethoxycarbonyl-8-methoxy-2,3,4,4a,5,9b-hexahydro-1H-pyrido[4,3-b] indolinium dichloride) on pentose phosphate pathway (PPP) and glutathione-dependent enzyme activities in aged diabetic and aged matched control rats. Diabetes was induced by streptozotocin injection in rats aged 13-15 months. Diabetic and control rats were divided into two subgroups, one untreated and one treated with SMe1EC2 (10 mg/kg/day, orally) for 4 months. SMe1EC2 ameliorated body weight loss, but not hyperglycemia of aged diabetic rats. Diabetes resulted in decreased glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD) and glutathione-S-transferase (GST), yet in unchanged glutathione reductase (GR) in the liver of aged diabetic rats. In the liver of the aged control rats, SMe1EC2 did not affe...

The Turkish Journal of Pharmaceutical Sciences

Amaç: Metabolik ve kronik bir hastalık olan diyabetin birçok ülkede önemli sağlık sorunu olma dur... more Amaç: Metabolik ve kronik bir hastalık olan diyabetin birçok ülkede önemli sağlık sorunu olma durumu artış göstermektedir. Diyabetin artmış serbest radikallerin üretimi ve azalmış antioksidan kapasiteyle ilişkili olduğu iddia edilmektedir. Diyabette oluşan oksiatif stres dokularda DNA hasarına neden olabilir. Antioksidan, antikanser, antiülser özellikler gibi sağlığa yararlı etkileri nedeniyle geleneksel Çin halk tıbbında yaygın olarak kullanılan ursolik asit çok iyi tanımlanmış pentasiklik triterpendir. Bu çalışmanın amacı, streptozotosin ile diyabet oluşturulmuş Wistar albino sıçanların böbreklerine ursolik asidin etkisini değerlendirmektir. Gereç ve Yöntemler: Bu amaçla, böbrek hücrelerindeki DNA hasarı alkali comet yöntemiyle araştırılmıştır. KAT, SOD, GR ve GSH-Px enzim aktiviteleri ve toplam GSH ve MDA düzeyleri gibi oksidatif stres parametreleri de ölçülmüştür. Bulgular: Ursolik asit tedavisinin, DNA hasarını, GR enzim aktivitelerini ve MDA seviyelerini önemli ölçüde azalttığı ve diyabetik sıçanlarda GSH düzeylerini ve CAT, SOD ve GSH-Px enzim aktivitelerini önemli ölçüde artırdığı bulundu. Sonuç: Sonuçlarımıza göre, ursolik asidin diyabetin neden olduğu böbrek hasarında yararlı olabileceği görülmüştür. Anahtar kelimeler: Diyabet, DNA hasarı, oksidatif stres, böbrek Objectives: Diabetes, a heteregenous metabolic and chronic disease, is a growing health problem in most countries. It has been claimed that diabetes is associated with the increased formation of free radicals and decreased in antioxidant potential. Oxidative stress formed in diabetes may cause DNA damage in the tissues. Ursolic acid, a well-known pentacylic triterpene, is commonly used in traditional Chinese medicine due to its beneficial health effects such as antioxidant, anticancer, and antiulcer properties. The aim of this study was to investigate the effects of ursolic acid in the kidneys of Wistar albino rats with streptozotocin-induced diabetes. Materials and Methods: DNA damage was evaluated in the kidney cells of rats using alkaline comet assays. Oxidative stress parameters such as CAT, SOD, GR, and GSH-Px enzyme activities and total GSH and MDA levels were also evaluated. Results: Ursolic acid treatment was found to significantly decrease DNA damage, GR enzyme activities, and MDA levels, and significantly increase GSH levels and CAT, SOD and GSH-Px enzyme activities in diabetic rats. Conclusion: According to our results, it seems that ursolic acid may be beneficial against diabetes-induced renal damage.

Turkish journal of medical sciences, Jan 23, 2018

Background/aim: Diabetes mellitus (DM) is a major health problem worldwide. Cinnamic acid (CA) an... more Background/aim: Diabetes mellitus (DM) is a major health problem worldwide. Cinnamic acid (CA) and its derivatives are synthesized in plants and increasing attention has been given to them in recent years due to the high number of beneficial health properties attributed to their consumption. The aim of this study was to investigate the effects of CA on streptozotocin-induced diabetes in Wistar albino rats. Materials and methods: DNA damage was evaluated in the blood, liver, and kidney cells of rats by the alkaline comet assay. Oxidative stress parameters such as catalase, superoxide dismutase, glutathione reductase, glutathione-S-transferase, and glutathione peroxidase activities and 8-hydroxy-2-deoxyguanosine, total glutathione, and malondialdehyde levels; biochemical parameters including insulin, total bilirubin, and BCA protein levels; hepatic enzyme levels such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase; and lipi...

Food and Chemical Toxicology

Turkiye Klinikleri Journal of Pharmacy Sciences, 2017

Turkiye Klinikleri Journal of Internal Medical Sciences, 2005

Turkiye Klinikleri Journal of Cosmetic Dermatology Special Topics, 2008

Turkiye Klinikleri Journal of Internal Medical Sciences, 2005

Turkiye Klinikleri Journal of Medical Sciences, 2008