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Papers by arti ghabru
Journal of eco-friendly agriculture, Dec 31, 2022
The rhizosphere contains a host of microorganisms that are affected by both abiotic stress and bi... more The rhizosphere contains a host of microorganisms that are affected by both abiotic stress and biotic stress. Low fertility is a major problem in establishing vegetation in degraded areas. Nitrogen (N) is an important plant nutrient needed for plant growth in abundance in the earth's atmosphere however; most tropical soils are deficient in available N. In low-lying ecosystems and without human fertilization or supplementation, the nutrients found in plants come from organic matter or organic matter. The amount of fixed nitrogen depends on the nitrogen level of the soil, the type of rhizobia that attacks the pods, the growth of the plant and the length of the growing season. Nitrogen-fixing tree species can play an important role in stabilizing sandy and eroded soils, utilizing deep groundwater due to its broad roots and thus helping to increase soil fertility. Among the indigenous legume species Albizia procera is best known for the traditional legume species, which are associated with Rhizobium and this association leads to nitrogen fixation in the atmosphere. Soil rhizobial value calculations showed high number of bacteria in the soil sample of Baddi (297.21 cfu g-1) and a small amount in Solan (243.46 cfu g-1). On the basis of verification tests namely, congo red test, ketolactose testing and plant infection testing in all isolated areas, more than 70.58 per cent (26 of 34) were confirmed as rhizoba. All isolates are tested for factors that promote the growth of different plants, namely, p-solubilizers, siderophore producers and IAA producers. Of these 26 rhizobial isolate, 82.38 per cent were p-solubilizers, 54.45 per cent were siderophore producers and 75.47 per cent were IAA producers. This approach was to improve the bio-inoculum to increase soil fertility by injecting the most suitable type of bacteria that would bring the best plant into the natural environment without adding fertilizer from the outside. Rhizobia is very important in improving the availability of nitrogen in the soil.
International journal of plant and soil science, Dec 31, 2023
The Pharma Innovation Journal, 2017
Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world... more Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world enzyme production. The amylases have applications in juice processing, starch processing, desizing of textiles, paper sizing, detergent additives, utilization of waste biomass for valuable products, treatment of waste water and other fermentation processes including malting barley and bakery industries. Amylases can generally extract from plants, microorganisms, fruits etc. Microorganisms serve as a potential source of amylase production. The study was carried out to isolate the amylase producing bacteria from spent mushroom compost from Nauni, of Himachal Pradesh. Subsequently, their screening and characterization was done on the basis of morphological, biochemical and molecular parameters. Out of 3isolates 1 bacterial isolate viz., M13 was identified as amylase producing. The highest amylase activity was obtained in apple pomace as a substrate at pH 9.0, 72 hrs of incubation and 45 o C temperature. Among different carbon sources starch followed by maltose was found to be the best. Among the nitrogen sources, Yeast extract (organic source) followed by NaNO3 for both the isolates was found best. The phylogenetic analysis of 16S rDNA sequence showed isolate M13 belongs to Bacillus sp. vide accession number [KY962809] obtained from NCBI gene bank.
Annals of phytomedicine, Dec 1, 2022
Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it... more Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it is necessary to provide scientific evidence to increase utilization of therapeutic flora in current medication. One of the most common Diplazium esculentum (Retz.) Sw. is used to make vegetables, pickles, salad and remedial drives. This review focuses on the nutritional benefits of D. esculentum. It contained different phytochemicals such as carbohydrates, free fatty acids, phenols, flavonoids and alkaloids. The leafy fern also contained essential micronutrients such as beta-carotene, folic acid and possess antioxidants, antimicrobial, immunomodulatory and antidiabetic activity. The nutritional qualities of this plant will aid in locating a potential source of a remedy for the nutritional issue and aid in biodiversity preservation. Fern provides potential for usage in food or nutritional programmes because it is nutrient-rich.
International journal of pharmaceutical science and research, Jul 1, 2018
The objective of the present study was to isolate, identify and optimize potential amylase produc... more The objective of the present study was to isolate, identify and optimize potential amylase producing bacteria and actinomycetes from soil samples. The soil samples were collected from Maraki and Tewedros campus, University of Gondar. Isolation was done by serial dilution and spread plate method. Primary screening of amylolytic activity of the isolates was performed by starch agar plate method. The submerged state fermentation method followed for the production of amylase by the optimization of temperature, pH, fermentation time and substrate concentration. From the soil samples, 18 isolates were identified and subjected to primary screening for amylolytic activity. Of which, five isolates were observed with maximum amylolytic activity during the primary screening. During the submerged state fermentation, maximum amylase activity was observed at 48 h and then declined. The optimum temperature observed for maximum amylase activity of Bacillus was 40°C and Streptomyces at 37°C. The highest amylase activity was observed at neutral pH and 4% of starch concentration. The colony morphology, Gram reaction, biochemical tests and Bergey's manual of determinative bacteriology confirm the promising isolates belong to the genus Bacillus and Streptomyces. This preliminary study could provide base line information for the discovery of novel microbes from the natural resources for the production of amylase which will be used for multipurpose.
The Pharma Innovation
Intensive agriculture which depends on unmanageable processes of agrochemical inputs is environme... more Intensive agriculture which depends on unmanageable processes of agrochemical inputs is environmentally dangerous. The development of these practices to fulfil needs isn't always economically viable. Different practical aspects must be taken into consideration to satisfy the global meals protection undertaking. The plant microbiome has been related to stepped forwards plant productiveness for decades. Rhizospheric bacteria were studied for their capability to promote crop growth and control pathogens. In recent years studies on endophytes have accelerated as a likely alternative to rhizobacteria, for the improvement of Microbial inoculants able to changing some agrochemicals and reducing the environmental impact of agronomic management of crops. This review summarizes the maximum vital characteristics and traits of endophytic microorganism. The presence of rhizomes in soil favours the boom of numerous microbial groups in its rhizosphere. Presently endophytic microorganisms are gaining attention through researchers because of their functionality to synthesizing novel bioactive compounds which might be useful in ailment management of phytopathogens, and some of these compounds are critical in novel drug discovery. For sustainable agriculture, a number of the bacterial and fungal endophytes can be used as plant and soil inoculants to enhance yield and productiveness of plants. Using endophytes as biofertilizers is beneficial and has no unfavorable consequences on the weather, or texture and productiveness of soils, unlike chemical fertilizers. These endophytes may be act as essential biofertilizers, biocontrol agent and help plants to cope up with biotic and abiotic stresses.
Asian Journal of Advances in Agricultural Research
Agriculture is an essential component to maintain the human health but due to sudden increase in ... more Agriculture is an essential component to maintain the human health but due to sudden increase in population the demand has increased and to meet the requirement the use of chemical fertilizer has also increase. The continuous use of chemical fertilizer and pesticides degrade the quality and fertility of soil and also reduce the yield of various crops. The organic sources such as panchgavya, jeevamrit and Farm Yard Manure consists load of beneficial microorganisms that promote the plant growth, yield and promote natural resources. The present study was carried out by isolating the bacteria from organic sources (panchgavya, jeevamrit and FYM) because minute amount of bacterial culture is suffient to prepare large one according to need and can be stored for longer time as compare to organic input. The samples of panchgavya, jeevamrit and FYM were collected from different location of Himachal Pradesh. A total of 32 bacterial isolates from panchgavya, 43 from jeevamrit and 59 from FYM we...
Journal of Soil and Water Conservation
Annals of Phytomedicine: An International Journal
Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it... more Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it is necessary to provide scientific evidence to increase utilization of therapeutic flora in current medication. One of the most common Diplazium esculentum (Retz.) Sw. is used to make vegetables, pickles, salad and remedial drives. This review focuses on the nutritional benefits of D. esculentum. It contained different phytochemicals such as carbohydrates, free fatty acids, phenols, flavonoids and alkaloids. The leafy fern also contained essential micronutrients such as beta-carotene, folic acid and possess antioxidants, antimicrobial, immunomodulatory and antidiabetic activity. The nutritional qualities of this plant will aid in locating a potential source of a remedy for the nutritional issue and aid in biodiversity preservation. Fern provides potential for usage in food or nutritional programmes because it is nutrient-rich.
Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Nitro... more Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Nitrogen fixation is the reduction of N2 (atmospheric nitrogen) to NH3 (ammonia) which is made possible by the enzyme nitrogenase. Strains of root-nodulating bacteria were isolated from root a nodule of Albizia. After confirmation test with yeast mannitol agar (YMA), isolates were collected for morphological and biochemical characterization. Twenty six nodule isolates from Albizia procera grown in YEM broth were studied for their physiological and biochemical characteristics. Most isolates tolerated high salt concentration (5% NaCl), able to grow at a temperature of 45 °C, survive in a pH range from 5 to 8 upto incubation of 120hrs.
Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world... more Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world enzyme production. The amylases have applications in juice processing, starch processing, desizing of textiles, paper sizing, detergent additives, utilization of waste biomass for valuable products, treatment of waste water and other fermentation processes including malting barley and bakery industries. Amylases can generally extract from plants, microorganisms, fruits etc. Microorganisms serve as a potential source of amylase production. The study was carried out to isolate the amylase producing bacteria from spent mushroom compost from Nauni, of Himachal Pradesh. Subsequently, their screening and characterization was done on the basis of morphological, biochemical and molecular parameters. Out of 3isolates 1 bacterial isolate viz., M13 was identified as amylase producing. The highest amylase activity was obtained in apple pomace as a substrate at pH 9.0, 72 hrs of incubation and 45C t...
Due to the increasing demand for enzymes in various industries, there is enormous interest in res... more Due to the increasing demand for enzymes in various industries, there is enormous interest in research on enzymes suitable for commercial applications and their cost effective production techniques. Thermophilic microorganisms have gained a great deal of attention. Hence, the enzyme from these microorganisms is of special interest because these are not usually denatured by high temperature and are active at elevated temperature. The amylase from thermophilic bacteria of hot springs which are supposed to be unexploited niches may have wide industrial applications. The application of extracted amylase from the bacterial isolates was evaluated for apple and kiwi juice yield and clarification. An application of 0.75 per cent of amylase yielded 58 per cent of apple juice and yielded 54 percent of kiwi juice from J2 isolate. The bacterial amylases were also evaluated for the preparation of bun where the maximum leavening activity of 2.60 ml/h at 0.75 per cent concentration for J2. Further, quality of buns from the selected concentration was also recorded. The loaf volume was recorded to be 177.43 cm 3 and 179.11cm 3 for J2 at the amylase concentration of 0.75 per cent. Hence, amylase yield, stability and the low cost substrate production supported the hypothesis that microbial enzymes have potential in food industries. These natural resources need to be exploited for commercial enzymes.
International Journal of Current Microbiology and Applied Sciences, 2017
For more than 1000 years green tea has been used in China and Japan as a medical herb and a healt... more For more than 1000 years green tea has been used in China and Japan as a medical herb and a healthful beverage. The traditional Chinese medicine has recommended this plant for headaches, body aches and pains, digestion, depression, detoxification, as an energizer and, in general, to prolong life. Tea is made from young shoots and leaves of the plant Camellia sinensis (Willson and Clifford 1992; Cabrera et al., 2006; Sharma et al., 2007).
In present study pattern of variation among seeds, pods and germination behavior of Albizia proce... more In present study pattern of variation among seeds, pods and germination behavior of Albizia procera seeds, collected from different provenances of Himachal Pradesh, was evaluated. Various locations of Himachal Pradesh were visited to mark and select the trees of Albizia procera for collection of planting material. Morphometricl characters of collected pods and seeds as well as germination behavior of seeds were studied to select the best seed source for plantation and agroforestry programs. Various pod and seed parameters viz., pod length (cm), number of seeds per pod, pod weight of 100 pods (gm), pod width (cm), pod thickness (cm) and seeds viz., seed length (cm), seed weight of 100 seeds (gm), seed width (cm) and seed thickness (cm) were evaluated for selection of quality seeds. Further the seeds from best seed source were tested for the determination of viability. Best response for all pod and seed parameters were obtained with seeds from Baddi. So, on the basis of results obtain...
Journal of Pharmacognosy and Phytochemistry, 2018
Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the worl... more Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the world. All cultivated tea is generally assigned to one species Camellia sinensis (L) O Kuntze, most of the tea grown in the world today is highly heterogeneous as a result of the large-scale dispersal of the tea plant during the long history of its cultivation, its out-breeding nature, and the free hybridization between geographical races. Based on the extent of fermentation the tea shoots undergo during processing, teas divided into three major groups: unfermented tea (green tea), semi-fermented tea (oolong tea) and fully fermented tea (black tea). Fresh tea leaves are rich in flavonoids - a group of phenolic compounds known as catechins. Tea is being advocated to be a functional food and an important source of dietary flavonoids. Epidemiological studies carried out during the last three decades suggested that green tea catechins have nutraceutical and therapeutic attributes. Green tea cate...
![Research paper thumbnail of Qualitative and quantitative evaluation of flavanols in Green tea [Camellia sinensis (L) O Kuntze]](https://attachments.academia-assets.com/77869208/thumbnails/1.jpg)
](The Pharma Innovation Journal, 2017
Plants are commonly used in treating or preventing specific ailments or diseases and are playing ... more Plants are commonly used in treating or preventing specific ailments or diseases and are playing valuable role in health care. Probably, around 60% of world’s population is relying on medicinal plants for their primary healthcare. Bioactive plant extracts are considered as a promising source of biological friendly antibacterial agents. The relationship between the quality and chemical components in green tea have been studied, and have shown that free amino acids, caffeine and polyphenols are qualitatively important components. Especially, catechins, the main component of polyphenols, are well known for their antioxidant properties, which have led to their evaluation in many diseases associated with free radicals. Significant seasonal variations of phenolic content were observed. The order of variations of flavan-3-ols was (–)-epigallocatechin gallate > (–)-epigallocatechin > (–)-epicatechin gallate > epicatechin > (+)-catechin.
Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the worl... more Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the world. Tea originated in China where its legendary history dates to as far back as 2737 B. C. Several centuries later, tea was brought to Japan in the 6th century and to Europe in 1559 A. D. (Wickremasinghe 1978). Although the first tea plantation was established in 1839 with seeds brought from China; however, it is believed that the indigenous tea [Camellia sinesis var. assamica] was cultivated by one of the indigenous north-east tribes, Singhpo, since time immemorial. The East India Company was the first to develop plantation in North-East India which was subsequently extended across Brahmaputra valley, Darjeeling and other Himalayan hills in North India. In the equatorial South, tea cultivation was taken up in the Nilgiris, Karnataka and Highlands of Kerala, simultaneously (Jain 2007). Tea plant grows best in slightly acidic soil conditions under tropical and sub-tropical areas with adequ...
Lantana (Lantana camara L.) is a hepatotoxic plant with a number of medicinal properties. Lantade... more Lantana (Lantana camara L.) is a hepatotoxic plant with a number of medicinal properties. Lantadene A (LA), a pentacyclic triterpenoid present in the leaves of lantana plant is one of the most important bioactive constituents of this plant. Pentacyclic triterpenoids exhibit a number of bioactivities like antitumor, anti-HIV, anti-inflammatory, antitubercular, inhibition of nitric oxide production, inhibition of DNA polymerase and DNA topoisomerase. LA was isolated from lantana leaves and purified by batch processes, column chromatography and fractional crystallization. The purity as ascertained by HPLC analysis. The chemical structure was confirmed as 22β-angeloyloxy-3-oxoolean-12-en-28-oic acid by spectroscopic analysis. LA-DNA interaction was ascertained y electrophoresis. The observations on interaction of LA with DNA as evaluated by agrose gel electrophoresis were very interesting. LA masked the binding of ethidium bromide to DNA implying thereby that lantadene. An interacted wi...
The Journal of Phytopharmacology
In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthor... more In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthorn leaves on microsomal enzyme system, antioxidant enzymes and biochemical parameters i.e. serum triglyceride, total plasma protein, aminopyrine demethylase, aniline hydroxylase, NADPH cytochrome P450 reductase, catalase, LPO, superoxide dismutase, GSH, blood urea nitrogen (BUN) and creatinine levels in poultry. The poultry birds were divided into six groups containing six birds each. Aflatoxin (400 ppb) and seabuckthorn leaves (10000ppm) was administered continuously in poultry feed. Aflatoxin increased serum triglyceride, blood urea nitrogen and creatinine levels where as seabuckthorn leaves supplementation at 10000ppm significantly decreased triglyceride (P<0.05), blood urea nitrogen (P<0.05) and creatinine levels in birds. Toxin decreased liver, kidney and blood superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activity, whereas, seabuckthorn leaves (SBTL) increas...
Journal of eco-friendly agriculture, Dec 31, 2022
The rhizosphere contains a host of microorganisms that are affected by both abiotic stress and bi... more The rhizosphere contains a host of microorganisms that are affected by both abiotic stress and biotic stress. Low fertility is a major problem in establishing vegetation in degraded areas. Nitrogen (N) is an important plant nutrient needed for plant growth in abundance in the earth's atmosphere however; most tropical soils are deficient in available N. In low-lying ecosystems and without human fertilization or supplementation, the nutrients found in plants come from organic matter or organic matter. The amount of fixed nitrogen depends on the nitrogen level of the soil, the type of rhizobia that attacks the pods, the growth of the plant and the length of the growing season. Nitrogen-fixing tree species can play an important role in stabilizing sandy and eroded soils, utilizing deep groundwater due to its broad roots and thus helping to increase soil fertility. Among the indigenous legume species Albizia procera is best known for the traditional legume species, which are associated with Rhizobium and this association leads to nitrogen fixation in the atmosphere. Soil rhizobial value calculations showed high number of bacteria in the soil sample of Baddi (297.21 cfu g-1) and a small amount in Solan (243.46 cfu g-1). On the basis of verification tests namely, congo red test, ketolactose testing and plant infection testing in all isolated areas, more than 70.58 per cent (26 of 34) were confirmed as rhizoba. All isolates are tested for factors that promote the growth of different plants, namely, p-solubilizers, siderophore producers and IAA producers. Of these 26 rhizobial isolate, 82.38 per cent were p-solubilizers, 54.45 per cent were siderophore producers and 75.47 per cent were IAA producers. This approach was to improve the bio-inoculum to increase soil fertility by injecting the most suitable type of bacteria that would bring the best plant into the natural environment without adding fertilizer from the outside. Rhizobia is very important in improving the availability of nitrogen in the soil.
International journal of plant and soil science, Dec 31, 2023
The Pharma Innovation Journal, 2017
Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world... more Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world enzyme production. The amylases have applications in juice processing, starch processing, desizing of textiles, paper sizing, detergent additives, utilization of waste biomass for valuable products, treatment of waste water and other fermentation processes including malting barley and bakery industries. Amylases can generally extract from plants, microorganisms, fruits etc. Microorganisms serve as a potential source of amylase production. The study was carried out to isolate the amylase producing bacteria from spent mushroom compost from Nauni, of Himachal Pradesh. Subsequently, their screening and characterization was done on the basis of morphological, biochemical and molecular parameters. Out of 3isolates 1 bacterial isolate viz., M13 was identified as amylase producing. The highest amylase activity was obtained in apple pomace as a substrate at pH 9.0, 72 hrs of incubation and 45 o C temperature. Among different carbon sources starch followed by maltose was found to be the best. Among the nitrogen sources, Yeast extract (organic source) followed by NaNO3 for both the isolates was found best. The phylogenetic analysis of 16S rDNA sequence showed isolate M13 belongs to Bacillus sp. vide accession number [KY962809] obtained from NCBI gene bank.
Annals of phytomedicine, Dec 1, 2022
Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it... more Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it is necessary to provide scientific evidence to increase utilization of therapeutic flora in current medication. One of the most common Diplazium esculentum (Retz.) Sw. is used to make vegetables, pickles, salad and remedial drives. This review focuses on the nutritional benefits of D. esculentum. It contained different phytochemicals such as carbohydrates, free fatty acids, phenols, flavonoids and alkaloids. The leafy fern also contained essential micronutrients such as beta-carotene, folic acid and possess antioxidants, antimicrobial, immunomodulatory and antidiabetic activity. The nutritional qualities of this plant will aid in locating a potential source of a remedy for the nutritional issue and aid in biodiversity preservation. Fern provides potential for usage in food or nutritional programmes because it is nutrient-rich.
International journal of pharmaceutical science and research, Jul 1, 2018
The objective of the present study was to isolate, identify and optimize potential amylase produc... more The objective of the present study was to isolate, identify and optimize potential amylase producing bacteria and actinomycetes from soil samples. The soil samples were collected from Maraki and Tewedros campus, University of Gondar. Isolation was done by serial dilution and spread plate method. Primary screening of amylolytic activity of the isolates was performed by starch agar plate method. The submerged state fermentation method followed for the production of amylase by the optimization of temperature, pH, fermentation time and substrate concentration. From the soil samples, 18 isolates were identified and subjected to primary screening for amylolytic activity. Of which, five isolates were observed with maximum amylolytic activity during the primary screening. During the submerged state fermentation, maximum amylase activity was observed at 48 h and then declined. The optimum temperature observed for maximum amylase activity of Bacillus was 40°C and Streptomyces at 37°C. The highest amylase activity was observed at neutral pH and 4% of starch concentration. The colony morphology, Gram reaction, biochemical tests and Bergey's manual of determinative bacteriology confirm the promising isolates belong to the genus Bacillus and Streptomyces. This preliminary study could provide base line information for the discovery of novel microbes from the natural resources for the production of amylase which will be used for multipurpose.
The Pharma Innovation
Intensive agriculture which depends on unmanageable processes of agrochemical inputs is environme... more Intensive agriculture which depends on unmanageable processes of agrochemical inputs is environmentally dangerous. The development of these practices to fulfil needs isn't always economically viable. Different practical aspects must be taken into consideration to satisfy the global meals protection undertaking. The plant microbiome has been related to stepped forwards plant productiveness for decades. Rhizospheric bacteria were studied for their capability to promote crop growth and control pathogens. In recent years studies on endophytes have accelerated as a likely alternative to rhizobacteria, for the improvement of Microbial inoculants able to changing some agrochemicals and reducing the environmental impact of agronomic management of crops. This review summarizes the maximum vital characteristics and traits of endophytic microorganism. The presence of rhizomes in soil favours the boom of numerous microbial groups in its rhizosphere. Presently endophytic microorganisms are gaining attention through researchers because of their functionality to synthesizing novel bioactive compounds which might be useful in ailment management of phytopathogens, and some of these compounds are critical in novel drug discovery. For sustainable agriculture, a number of the bacterial and fungal endophytes can be used as plant and soil inoculants to enhance yield and productiveness of plants. Using endophytes as biofertilizers is beneficial and has no unfavorable consequences on the weather, or texture and productiveness of soils, unlike chemical fertilizers. These endophytes may be act as essential biofertilizers, biocontrol agent and help plants to cope up with biotic and abiotic stresses.
Asian Journal of Advances in Agricultural Research
Agriculture is an essential component to maintain the human health but due to sudden increase in ... more Agriculture is an essential component to maintain the human health but due to sudden increase in population the demand has increased and to meet the requirement the use of chemical fertilizer has also increase. The continuous use of chemical fertilizer and pesticides degrade the quality and fertility of soil and also reduce the yield of various crops. The organic sources such as panchgavya, jeevamrit and Farm Yard Manure consists load of beneficial microorganisms that promote the plant growth, yield and promote natural resources. The present study was carried out by isolating the bacteria from organic sources (panchgavya, jeevamrit and FYM) because minute amount of bacterial culture is suffient to prepare large one according to need and can be stored for longer time as compare to organic input. The samples of panchgavya, jeevamrit and FYM were collected from different location of Himachal Pradesh. A total of 32 bacterial isolates from panchgavya, 43 from jeevamrit and 59 from FYM we...
Journal of Soil and Water Conservation
Annals of Phytomedicine: An International Journal
Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it... more Despite hundreds of floras being utilized worldwide in the direction to cure/prevent ailments; it is necessary to provide scientific evidence to increase utilization of therapeutic flora in current medication. One of the most common Diplazium esculentum (Retz.) Sw. is used to make vegetables, pickles, salad and remedial drives. This review focuses on the nutritional benefits of D. esculentum. It contained different phytochemicals such as carbohydrates, free fatty acids, phenols, flavonoids and alkaloids. The leafy fern also contained essential micronutrients such as beta-carotene, folic acid and possess antioxidants, antimicrobial, immunomodulatory and antidiabetic activity. The nutritional qualities of this plant will aid in locating a potential source of a remedy for the nutritional issue and aid in biodiversity preservation. Fern provides potential for usage in food or nutritional programmes because it is nutrient-rich.
Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Nitro... more Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Nitrogen fixation is the reduction of N2 (atmospheric nitrogen) to NH3 (ammonia) which is made possible by the enzyme nitrogenase. Strains of root-nodulating bacteria were isolated from root a nodule of Albizia. After confirmation test with yeast mannitol agar (YMA), isolates were collected for morphological and biochemical characterization. Twenty six nodule isolates from Albizia procera grown in YEM broth were studied for their physiological and biochemical characteristics. Most isolates tolerated high salt concentration (5% NaCl), able to grow at a temperature of 45 °C, survive in a pH range from 5 to 8 upto incubation of 120hrs.
Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world... more Amylases constitute a class of industrial enzymes representing approximately 30 per cent of world enzyme production. The amylases have applications in juice processing, starch processing, desizing of textiles, paper sizing, detergent additives, utilization of waste biomass for valuable products, treatment of waste water and other fermentation processes including malting barley and bakery industries. Amylases can generally extract from plants, microorganisms, fruits etc. Microorganisms serve as a potential source of amylase production. The study was carried out to isolate the amylase producing bacteria from spent mushroom compost from Nauni, of Himachal Pradesh. Subsequently, their screening and characterization was done on the basis of morphological, biochemical and molecular parameters. Out of 3isolates 1 bacterial isolate viz., M13 was identified as amylase producing. The highest amylase activity was obtained in apple pomace as a substrate at pH 9.0, 72 hrs of incubation and 45C t...
Due to the increasing demand for enzymes in various industries, there is enormous interest in res... more Due to the increasing demand for enzymes in various industries, there is enormous interest in research on enzymes suitable for commercial applications and their cost effective production techniques. Thermophilic microorganisms have gained a great deal of attention. Hence, the enzyme from these microorganisms is of special interest because these are not usually denatured by high temperature and are active at elevated temperature. The amylase from thermophilic bacteria of hot springs which are supposed to be unexploited niches may have wide industrial applications. The application of extracted amylase from the bacterial isolates was evaluated for apple and kiwi juice yield and clarification. An application of 0.75 per cent of amylase yielded 58 per cent of apple juice and yielded 54 percent of kiwi juice from J2 isolate. The bacterial amylases were also evaluated for the preparation of bun where the maximum leavening activity of 2.60 ml/h at 0.75 per cent concentration for J2. Further, quality of buns from the selected concentration was also recorded. The loaf volume was recorded to be 177.43 cm 3 and 179.11cm 3 for J2 at the amylase concentration of 0.75 per cent. Hence, amylase yield, stability and the low cost substrate production supported the hypothesis that microbial enzymes have potential in food industries. These natural resources need to be exploited for commercial enzymes.
International Journal of Current Microbiology and Applied Sciences, 2017
For more than 1000 years green tea has been used in China and Japan as a medical herb and a healt... more For more than 1000 years green tea has been used in China and Japan as a medical herb and a healthful beverage. The traditional Chinese medicine has recommended this plant for headaches, body aches and pains, digestion, depression, detoxification, as an energizer and, in general, to prolong life. Tea is made from young shoots and leaves of the plant Camellia sinensis (Willson and Clifford 1992; Cabrera et al., 2006; Sharma et al., 2007).
In present study pattern of variation among seeds, pods and germination behavior of Albizia proce... more In present study pattern of variation among seeds, pods and germination behavior of Albizia procera seeds, collected from different provenances of Himachal Pradesh, was evaluated. Various locations of Himachal Pradesh were visited to mark and select the trees of Albizia procera for collection of planting material. Morphometricl characters of collected pods and seeds as well as germination behavior of seeds were studied to select the best seed source for plantation and agroforestry programs. Various pod and seed parameters viz., pod length (cm), number of seeds per pod, pod weight of 100 pods (gm), pod width (cm), pod thickness (cm) and seeds viz., seed length (cm), seed weight of 100 seeds (gm), seed width (cm) and seed thickness (cm) were evaluated for selection of quality seeds. Further the seeds from best seed source were tested for the determination of viability. Best response for all pod and seed parameters were obtained with seeds from Baddi. So, on the basis of results obtain...
Journal of Pharmacognosy and Phytochemistry, 2018
Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the worl... more Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the world. All cultivated tea is generally assigned to one species Camellia sinensis (L) O Kuntze, most of the tea grown in the world today is highly heterogeneous as a result of the large-scale dispersal of the tea plant during the long history of its cultivation, its out-breeding nature, and the free hybridization between geographical races. Based on the extent of fermentation the tea shoots undergo during processing, teas divided into three major groups: unfermented tea (green tea), semi-fermented tea (oolong tea) and fully fermented tea (black tea). Fresh tea leaves are rich in flavonoids - a group of phenolic compounds known as catechins. Tea is being advocated to be a functional food and an important source of dietary flavonoids. Epidemiological studies carried out during the last three decades suggested that green tea catechins have nutraceutical and therapeutic attributes. Green tea cate...
The Pharma Innovation Journal, 2017
Plants are commonly used in treating or preventing specific ailments or diseases and are playing ... more Plants are commonly used in treating or preventing specific ailments or diseases and are playing valuable role in health care. Probably, around 60% of world’s population is relying on medicinal plants for their primary healthcare. Bioactive plant extracts are considered as a promising source of biological friendly antibacterial agents. The relationship between the quality and chemical components in green tea have been studied, and have shown that free amino acids, caffeine and polyphenols are qualitatively important components. Especially, catechins, the main component of polyphenols, are well known for their antioxidant properties, which have led to their evaluation in many diseases associated with free radicals. Significant seasonal variations of phenolic content were observed. The order of variations of flavan-3-ols was (–)-epigallocatechin gallate > (–)-epigallocatechin > (–)-epicatechin gallate > epicatechin > (+)-catechin.
Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the worl... more Tea, throughout its history, has been one of the most popular non-alcoholic beverages in the world. Tea originated in China where its legendary history dates to as far back as 2737 B. C. Several centuries later, tea was brought to Japan in the 6th century and to Europe in 1559 A. D. (Wickremasinghe 1978). Although the first tea plantation was established in 1839 with seeds brought from China; however, it is believed that the indigenous tea [Camellia sinesis var. assamica] was cultivated by one of the indigenous north-east tribes, Singhpo, since time immemorial. The East India Company was the first to develop plantation in North-East India which was subsequently extended across Brahmaputra valley, Darjeeling and other Himalayan hills in North India. In the equatorial South, tea cultivation was taken up in the Nilgiris, Karnataka and Highlands of Kerala, simultaneously (Jain 2007). Tea plant grows best in slightly acidic soil conditions under tropical and sub-tropical areas with adequ...
Lantana (Lantana camara L.) is a hepatotoxic plant with a number of medicinal properties. Lantade... more Lantana (Lantana camara L.) is a hepatotoxic plant with a number of medicinal properties. Lantadene A (LA), a pentacyclic triterpenoid present in the leaves of lantana plant is one of the most important bioactive constituents of this plant. Pentacyclic triterpenoids exhibit a number of bioactivities like antitumor, anti-HIV, anti-inflammatory, antitubercular, inhibition of nitric oxide production, inhibition of DNA polymerase and DNA topoisomerase. LA was isolated from lantana leaves and purified by batch processes, column chromatography and fractional crystallization. The purity as ascertained by HPLC analysis. The chemical structure was confirmed as 22β-angeloyloxy-3-oxoolean-12-en-28-oic acid by spectroscopic analysis. LA-DNA interaction was ascertained y electrophoresis. The observations on interaction of LA with DNA as evaluated by agrose gel electrophoresis were very interesting. LA masked the binding of ethidium bromide to DNA implying thereby that lantadene. An interacted wi...
The Journal of Phytopharmacology
In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthor... more In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthorn leaves on microsomal enzyme system, antioxidant enzymes and biochemical parameters i.e. serum triglyceride, total plasma protein, aminopyrine demethylase, aniline hydroxylase, NADPH cytochrome P450 reductase, catalase, LPO, superoxide dismutase, GSH, blood urea nitrogen (BUN) and creatinine levels in poultry. The poultry birds were divided into six groups containing six birds each. Aflatoxin (400 ppb) and seabuckthorn leaves (10000ppm) was administered continuously in poultry feed. Aflatoxin increased serum triglyceride, blood urea nitrogen and creatinine levels where as seabuckthorn leaves supplementation at 10000ppm significantly decreased triglyceride (P<0.05), blood urea nitrogen (P<0.05) and creatinine levels in birds. Toxin decreased liver, kidney and blood superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activity, whereas, seabuckthorn leaves (SBTL) increas...