awal meer - Academia.edu (original) (raw)
Papers by awal meer
Journal of Medical Sciences, Sep 15, 2015
Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests whi... more Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests which are currently used in tertiary care hospitals for the indentification of S aureus and to identify an optimal phenotypic test that is reliagle and cost effective and can be used with confidence for confirmation of S. aureus. Material and Methods: The present study was conducted at clinical microbiology laboratory department of Pathology of Northwest General Hospital and Research Centre, Peshawar from January 2012 to September 2013. The study group consisted of 300 samples were collected from different clinical sources i.e. patient's blood, body fluids, pus swabs, wound swabs, urine and sputum. During sampling safety methods were adopted and data were collect regarding age, sex, types of specimens and present health condition were also recorded. Results: All clinical samples evaluated with slide coagulase (SCT), mannitol salt fermented test (MSA) and DNase test the result were 95%, 87% and 86%, respectively. Combination of these tests with different sera used in SCT their sensitivity results increased considerably. The human plasma when used in SCT test the positive results were 95%, but when used in combination with MSA test and DNase test then sensitivity increased to 98% and 96%, respectively. Similarly, while using horse plasma, 90% were slide coagulase positive and combination of SCT with mannitol test and DNase test depicted 94% and 91% positive results respectively. In similar manner, when cow plasma was used, slide Coagulase test showed 85% sensitivity and in combination with mannitol fermentation test and DNase test depicted 87% and 86% sensitivity, respectively. Conclusion: It is concluded that there is no single phenotypic test that can give 100% reliable identification of S. aureus and we have to use combination of tests for maximum reliable results. The combination of slide coagulase test with mannitol fermentation test provide the maximum reliable results while using human plasma followed by using horse and cow plasma.
Pakistan Journal of Medical and Health Sciences, Jun 30, 2022
Background: Parasitic infection is one of the most common infections in school age children becau... more Background: Parasitic infection is one of the most common infections in school age children because of poor quality of water, deprived sanitation system and lack of enough health care facilities in underdeveloped countries. Parasitic infections lead to anemia, growth retardation, weight loss, and other physical and mental health problems in children. Current study is designed to evaluate frequency of different intestinal parasitic infections and possible associated risk factors among school students in of District Buner Pakistan. Material and method: This Descriptive cross-sectional study was conducted at Riaz Clinical Laboratory District Buner. All asymptomatic school going children between 5 to 15 years' age of both genders were included in the study. Those students who had present with symptoms of gastrointestinal infection were excluded from the study. A total of 440 samples were collected from different public and private school's children. Out of total 240 samples were collected from public sector schools and 200 samples from private sector school's children. A pretested structured questionnaire was used to gather data on sociodemographic and associated risk factors. The adequate stool specimens were collected in sterile, screw caped disposable plastic container. All samples were examined under microscope using a direct wet mount and formal ether concentration technique. Data was analyzed through SPSS-22 and presented in the form of tables, Pie chart and bar graphs. Results: Out of 440 samples, 23.63% (104/440) were positive for an ova and cysts of different parasites. The parasite positivity ratio is greater in public sector school children (30%) compare to private sector school children (16%). Taenia saginata were most common parasite (8.4%) in school age children followed by Hymenolepis nana (5.68%), Ascaris lumbricoides (5.45%), Enterobius vermicularis 1.13%, and Strongyloides stecoralis (0.68%) were least common parasite in District Buner, Khyber Pukhtoonkhwa. Conclusion: Major contributor for the high prevalence of parasitic infections in public school children were found to be poor personal and environmental hygiene, lack of proper sanitation, contaminated food and water supply, personal habits of the children like nail biting and finger sucking, illiterate mother and low socioeconomic status of the individuals.
Pakistan Journal of Medical and Health Sciences
Background: Parasitic infection is one of the most common infections in school age children becau... more Background: Parasitic infection is one of the most common infections in school age children because of poor quality of water, deprived sanitation system and lack of enough health care facilities in underdeveloped countries. Parasitic infections lead to anemia, growth retardation, weight loss, and other physical and mental health problems in children. Current study is designed to evaluate frequency of different intestinal parasitic infections and possible associated risk factors among school students in of District Buner Pakistan. Material and method: This Descriptive cross-sectional study was conducted at Riaz Clinical Laboratory District Buner. All asymptomatic school going children between 5 to 15 years’ age of both genders were included in the study. Those students who had present with symptoms of gastrointestinal infection were excluded from the study. A total of 440 samples were collected from different public and private school’s children. Out of total 240 samples were collect...
Journal of Medical Sciences, 2015
Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests whi... more Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests which are currently used in tertiary care hospitals for the indentification of S aureus and to identify an optimal phenotypic test that is reliagle and cost effective and can be used with confidence for confirmation of S. aureus.Material and Methods: The present study was conducted at clinical microbiology laboratory department of Pathology of Northwest General Hospital and Research Centre, Peshawar from January 2012 to September 2013. The study group consisted of 300 samples were collected from different clinical sources i.e. patient’s blood, body fluids, pus swabs, wound swabs, urine and sputum. During sampling safety methods were adopted and data were collect regarding age, sex, types of specimens and present health condition were also recorded.Results: All clinical samples evaluated with slide coagulase (SCT), mannitol salt fermented test (MSA) and DNase test the result were 95%, 87% an...
Journal of Medical Sciences, Sep 15, 2015
Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests whi... more Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests which are currently used in tertiary care hospitals for the indentification of S aureus and to identify an optimal phenotypic test that is reliagle and cost effective and can be used with confidence for confirmation of S. aureus. Material and Methods: The present study was conducted at clinical microbiology laboratory department of Pathology of Northwest General Hospital and Research Centre, Peshawar from January 2012 to September 2013. The study group consisted of 300 samples were collected from different clinical sources i.e. patient's blood, body fluids, pus swabs, wound swabs, urine and sputum. During sampling safety methods were adopted and data were collect regarding age, sex, types of specimens and present health condition were also recorded. Results: All clinical samples evaluated with slide coagulase (SCT), mannitol salt fermented test (MSA) and DNase test the result were 95%, 87% and 86%, respectively. Combination of these tests with different sera used in SCT their sensitivity results increased considerably. The human plasma when used in SCT test the positive results were 95%, but when used in combination with MSA test and DNase test then sensitivity increased to 98% and 96%, respectively. Similarly, while using horse plasma, 90% were slide coagulase positive and combination of SCT with mannitol test and DNase test depicted 94% and 91% positive results respectively. In similar manner, when cow plasma was used, slide Coagulase test showed 85% sensitivity and in combination with mannitol fermentation test and DNase test depicted 87% and 86% sensitivity, respectively. Conclusion: It is concluded that there is no single phenotypic test that can give 100% reliable identification of S. aureus and we have to use combination of tests for maximum reliable results. The combination of slide coagulase test with mannitol fermentation test provide the maximum reliable results while using human plasma followed by using horse and cow plasma.
Pakistan Journal of Medical and Health Sciences, Jun 30, 2022
Background: Parasitic infection is one of the most common infections in school age children becau... more Background: Parasitic infection is one of the most common infections in school age children because of poor quality of water, deprived sanitation system and lack of enough health care facilities in underdeveloped countries. Parasitic infections lead to anemia, growth retardation, weight loss, and other physical and mental health problems in children. Current study is designed to evaluate frequency of different intestinal parasitic infections and possible associated risk factors among school students in of District Buner Pakistan. Material and method: This Descriptive cross-sectional study was conducted at Riaz Clinical Laboratory District Buner. All asymptomatic school going children between 5 to 15 years' age of both genders were included in the study. Those students who had present with symptoms of gastrointestinal infection were excluded from the study. A total of 440 samples were collected from different public and private school's children. Out of total 240 samples were collected from public sector schools and 200 samples from private sector school's children. A pretested structured questionnaire was used to gather data on sociodemographic and associated risk factors. The adequate stool specimens were collected in sterile, screw caped disposable plastic container. All samples were examined under microscope using a direct wet mount and formal ether concentration technique. Data was analyzed through SPSS-22 and presented in the form of tables, Pie chart and bar graphs. Results: Out of 440 samples, 23.63% (104/440) were positive for an ova and cysts of different parasites. The parasite positivity ratio is greater in public sector school children (30%) compare to private sector school children (16%). Taenia saginata were most common parasite (8.4%) in school age children followed by Hymenolepis nana (5.68%), Ascaris lumbricoides (5.45%), Enterobius vermicularis 1.13%, and Strongyloides stecoralis (0.68%) were least common parasite in District Buner, Khyber Pukhtoonkhwa. Conclusion: Major contributor for the high prevalence of parasitic infections in public school children were found to be poor personal and environmental hygiene, lack of proper sanitation, contaminated food and water supply, personal habits of the children like nail biting and finger sucking, illiterate mother and low socioeconomic status of the individuals.
Pakistan Journal of Medical and Health Sciences
Background: Parasitic infection is one of the most common infections in school age children becau... more Background: Parasitic infection is one of the most common infections in school age children because of poor quality of water, deprived sanitation system and lack of enough health care facilities in underdeveloped countries. Parasitic infections lead to anemia, growth retardation, weight loss, and other physical and mental health problems in children. Current study is designed to evaluate frequency of different intestinal parasitic infections and possible associated risk factors among school students in of District Buner Pakistan. Material and method: This Descriptive cross-sectional study was conducted at Riaz Clinical Laboratory District Buner. All asymptomatic school going children between 5 to 15 years’ age of both genders were included in the study. Those students who had present with symptoms of gastrointestinal infection were excluded from the study. A total of 440 samples were collected from different public and private school’s children. Out of total 240 samples were collect...
Journal of Medical Sciences, 2015
Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests whi... more Objective: To evaluate performance (sensitivity & specificity) of a range of phenotypic tests which are currently used in tertiary care hospitals for the indentification of S aureus and to identify an optimal phenotypic test that is reliagle and cost effective and can be used with confidence for confirmation of S. aureus.Material and Methods: The present study was conducted at clinical microbiology laboratory department of Pathology of Northwest General Hospital and Research Centre, Peshawar from January 2012 to September 2013. The study group consisted of 300 samples were collected from different clinical sources i.e. patient’s blood, body fluids, pus swabs, wound swabs, urine and sputum. During sampling safety methods were adopted and data were collect regarding age, sex, types of specimens and present health condition were also recorded.Results: All clinical samples evaluated with slide coagulase (SCT), mannitol salt fermented test (MSA) and DNase test the result were 95%, 87% an...