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Papers by ayobami eluwole
Biomedicines
Type 2 diabetes mellitus (T2DM) is characterized by endothelial dysfunction, increased thrombogen... more Type 2 diabetes mellitus (T2DM) is characterized by endothelial dysfunction, increased thrombogenicity, and inflammation. The soluble human F11 receptor (sF11R) and annexin A5 (ANXA5) play crucial roles in inflammatory thrombosis and atherosclerosis. We examined the relationship between circulating sF11R and ANXA5 and their impact on endothelial function. The study included 125 patients with T2DM. Plasma levels of sF11R and ANXA5 were quantified by ELISA. Microvascular function was assessed using the vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (CIMT) was assessed by B-mode ultrasound imaging. The mean age of patients in the study was 59.7 ± 7.8 years, 78% had hypertension, 76% had dyslipidemia, and 12% had CKD. sF11R correlated positively with ANXA5 levels (β = 0.250, p = 0.005), and correlated inversely with VRI and total nitic oxide (NO), (β = −0.201, p = 0.024; β = −0.357, p = 0....
Hypertension, 2017
Rationale: Diabetes is highly prevalent among African Americans and poses a higher risk for vascu... more Rationale: Diabetes is highly prevalent among African Americans and poses a higher risk for vascular complications in this population. Although socioeconomic factors are well known to influence outcomes, true biologic differences in risk factor vulnerability have been suggested. Vascular complications have been traditionally viewed as either macrovascular (myocardial infarction and stroke) or microvascular (retinopathy, nephropathy, and neuropathy). Better glycemic control is known to improve microvascular but not macrovascular complications. In recent years, there has been a growing appreciation that microvascular dysfunction may promote large artery disease and vice versa. Given this notion of vascular “cross-talk” and since subclinical dysfunction is known to precede target organ damage, the objective of this study was to determine whether subclinical microvascular dysfunction is related to large artery stiffness. Methods: A total of 141 patients with type II diabetes were recrui...
Frontiers in Bioscience, 2021
mode ultrasound imaging. In univariate analysis, NT-HDL was associated with VRI in total populati... more mode ultrasound imaging. In univariate analysis, NT-HDL was associated with VRI in total population and in patients with HbA1c ≤7.0% (β=-0.178, p= 0.034; β=-0.265, p= 0.042; respectively). In contrast, NT-LDL was associated with CIMT in total population and in patients with HbA1c >7.0% (β=-0.205, p= 0.022; β=-0.244, p= 0.042; respectively). Multivariableadjusted regression analysis demonstrated that NT-HDL independently predicted VRI outcome in total population and in well-controlled patients (β=-0.282, p= 0.014; β=-0.400, p= 0.035, respectively). These results suggest that NT-HDL could be used as marker to identify diabetic patients at risk of developing early microvascular complications.
Diabetes, 2018
Background: LDL plays a key role in development and progression of atherosclerosis. Elevated LDL ... more Background: LDL plays a key role in development and progression of atherosclerosis. Elevated LDL amplifies the atherosclerotic process but the significance of modified LDL in pathogenesis of vascular complications is unclear. We aimed to assess plasma modified LDL levels and their relationship with vascular function in African American diabetic patients. Methods: 125 patients with type-2 diabetes were enrolled. Levels of glycated LDL (g-LDL), carbamylated LDL (c-LDL), nitrated LDL (n-LDL) and oxidized LDL (ox-LDL) were quantified by ELISA. Microvascular function was assessed by vascular reactivity index (VRI), which assesses changes in digital temperature before and after release of arterial cuff occlusion. Carotid-femoral pulse wave velocity (PWV) assessed arterial stiffness using applanation tonometry. B-mode ultrasound image analysis assessed Carotid intima-media thickness (CIMT). Patient population was divided into well-controlled: HbA1c ≤7.0%, N=54; poorly-controlled: HbA1c >...
Diabetes, 2018
Previous studies have shown that HDL isolated from human atherosclerotic lesions and the blood of... more Previous studies have shown that HDL isolated from human atherosclerotic lesions and the blood of patients with established coronary artery disease contains elevated levels of nitrated apolipoprotein AI (NT-apoAI). The significance of NT-apoAI in the pathogenesis of vascular diabetic complications is not well understood. We aimed to evaluate the relationship between plasma NT-apoAI and vascular function in African-Americans diabetic patients. 125 patients with type 2 diabetes were enrolled over a 6 months period. Levels of plasma NT-apoAI was quantified by enzyme-linked immunosorbent assays (ELISA). Microvascular function was assessed by vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (CIMT) was assessed by B-mode ultrasound image analysis. Patients population was divided in two groups (well-controlled: HbA1c ≤7.0%, N=54; poorly-controlled: HbA1c >7.0%, N=71). Mean age 60±8 years; 64...
Diabetes, 2018
Patients with type 2 diabetes mellitus exhibit non-enzymatic glycation of lipoproteins that are c... more Patients with type 2 diabetes mellitus exhibit non-enzymatic glycation of lipoproteins that are considered proatherogenic modification contributing to increased susceptibility of patients with diabetes to atherosclerosis. We postulated that glycated lipoproteins might be associated with vascular outcome. To explore this, we studied the relationship between glycated HDL (gHDL) and glycated LDL (gLDL) with vascular function in African American diabetic patients. Total of 146 diabetic were enrolled over a 6 month period. Levels of plasma gHDL and gLDL were measured by enzyme-linked immunosorbent assay (ELISA). Microvascular function was assessed by vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (C-IMT) was assessed by B-mode ultrasound image analysis. Mean patient age was 60±8 years, 64% were female. 80% had hypertension, 90% had dyslipidemia and 15% had chronic kidney disease. Mean HbA1c...
Diabetes, 2018
Background: Diabetes is a robust risk factor for cardiovascular events which is marked in African... more Background: Diabetes is a robust risk factor for cardiovascular events which is marked in African Americans for reasons that may be attributed to socioeconomic and biologic vulnerabilities. Both structural (arterial thickening) and functional (arterial stiffness) abnormalities of the vasculature are predictive of incidental clinical events. Study objective was to determine the risk factors and relationship between large artery stiffness and carotid intimal thickness in African Americans with type II diabetes. Methods: 125 patients with diabetes were recruited from medical clinics. Medical information was obtained via interview and electronic medical record review. Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV) using applanation tonometry. Carotid Intimal Medial Thickness (CIMT) was obtained using B-mode ultrasound image analysis of the common carotid artery. Statistical analysis was done using SPSS version 23. Results: Mean age 60±8 years, 64% femal...
Journal of the American College of Cardiology, 2018
Journal of the American College of Cardiology, 2019
Background: Human F11 receptor (F11R) is an important cell adhesion molecule implicated in inflam... more Background: Human F11 receptor (F11R) is an important cell adhesion molecule implicated in inflammatory thrombosis. F11R is associated with the presence and severity of cardiovascular disease. Endothelial dysfunction in diabetes has been attributed to lack of bioavailability of nitric oxide (NO), another key player of platelet adhesion and aggregation. Our objective was to examine the relationship between F11R and NO and their impact on vascular function in African Americans with diabetes. Methods: 146 patients with diabetes were recruited from medical clinics over 6-month period. Plasma F11R and total NO were measured by ELISA. Microvascular function was assessed by vascular reactivity index (VRI), which assess changes in digital temperature before and after release of arterial cuff occlusion. Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV) using applanation tonometry. Patient population was categorized into two groups: good control (HbA1c≤7; N=85) and poor control (HbA1c>7.0; N=61). Results: Age 60±8 years, female 64%, hypertension 82%, dyslipidemia 83%, diabetes duration 10.2±7.6 years, HbA1C= 8.1±2.2%. In the total population, F11R was negatively correlated with NO (r=-0.341, p=0.0001) and VRI (r=-0.191, P=0.022), but not PWV (r=0.082, p=0.335). In poor control group, F11R was significantly correlated with NO (r=-0.397, p=0.0001) but not VRI (r=-0.193, p=0.079). Univariate analysis showed plasma F11R predicted NO availability (β=-0.273, p=0.001) but not VRI (r=-0.234, p=0.475). Multiple linear regression analysis showed that F11R was independently associated with total NO levels (β=-0.339, p=0.009) after adjustment for age, gender, weight, hypertension, stroke, dyslipidemia, smoking, HbA1c, duration of diabetes, total cholesterol, LDLc, HDLc, triglycerides, medications. Conclusion: Our findings demonstrated significant inverse association between F11R and NO levels but neither were significantly associated with microvascular dysfunction or arterial stiffness in this cohort, suggesting F11R and NO may not be reliable markers of early vascular changes in diabetes. Larger prospective studies are needed to clarify these findings.
Journal of the American College of Cardiology, 2018
American Journal of Medical Case Reports, Jan 23, 2014
Circulation, 2019
Background: Endothelial dysfunction in diabetes has been attributed to lack of nitric oxide (NO) ... more Background: Endothelial dysfunction in diabetes has been attributed to lack of nitric oxide (NO) bioavailability. Oxidized LDL (OxLDL) and NO play antagonist roles in endothelium dysfunction. OxLDL...
Rationale: Diabetes mellitus is a risk factor for large and small vessel disease and portends a d... more Rationale: Diabetes mellitus is a risk factor for large and small vessel disease and portends a disproportionate morbidity and mortality burden in African Americans. Diabetes is often accompanied b...
Journal of Translational Science
Journal of Translational Science
Biomedicines
Type 2 diabetes mellitus (T2DM) is characterized by endothelial dysfunction, increased thrombogen... more Type 2 diabetes mellitus (T2DM) is characterized by endothelial dysfunction, increased thrombogenicity, and inflammation. The soluble human F11 receptor (sF11R) and annexin A5 (ANXA5) play crucial roles in inflammatory thrombosis and atherosclerosis. We examined the relationship between circulating sF11R and ANXA5 and their impact on endothelial function. The study included 125 patients with T2DM. Plasma levels of sF11R and ANXA5 were quantified by ELISA. Microvascular function was assessed using the vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (CIMT) was assessed by B-mode ultrasound imaging. The mean age of patients in the study was 59.7 ± 7.8 years, 78% had hypertension, 76% had dyslipidemia, and 12% had CKD. sF11R correlated positively with ANXA5 levels (β = 0.250, p = 0.005), and correlated inversely with VRI and total nitic oxide (NO), (β = −0.201, p = 0.024; β = −0.357, p = 0....
Hypertension, 2017
Rationale: Diabetes is highly prevalent among African Americans and poses a higher risk for vascu... more Rationale: Diabetes is highly prevalent among African Americans and poses a higher risk for vascular complications in this population. Although socioeconomic factors are well known to influence outcomes, true biologic differences in risk factor vulnerability have been suggested. Vascular complications have been traditionally viewed as either macrovascular (myocardial infarction and stroke) or microvascular (retinopathy, nephropathy, and neuropathy). Better glycemic control is known to improve microvascular but not macrovascular complications. In recent years, there has been a growing appreciation that microvascular dysfunction may promote large artery disease and vice versa. Given this notion of vascular “cross-talk” and since subclinical dysfunction is known to precede target organ damage, the objective of this study was to determine whether subclinical microvascular dysfunction is related to large artery stiffness. Methods: A total of 141 patients with type II diabetes were recrui...
Frontiers in Bioscience, 2021
mode ultrasound imaging. In univariate analysis, NT-HDL was associated with VRI in total populati... more mode ultrasound imaging. In univariate analysis, NT-HDL was associated with VRI in total population and in patients with HbA1c ≤7.0% (β=-0.178, p= 0.034; β=-0.265, p= 0.042; respectively). In contrast, NT-LDL was associated with CIMT in total population and in patients with HbA1c >7.0% (β=-0.205, p= 0.022; β=-0.244, p= 0.042; respectively). Multivariableadjusted regression analysis demonstrated that NT-HDL independently predicted VRI outcome in total population and in well-controlled patients (β=-0.282, p= 0.014; β=-0.400, p= 0.035, respectively). These results suggest that NT-HDL could be used as marker to identify diabetic patients at risk of developing early microvascular complications.
Diabetes, 2018
Background: LDL plays a key role in development and progression of atherosclerosis. Elevated LDL ... more Background: LDL plays a key role in development and progression of atherosclerosis. Elevated LDL amplifies the atherosclerotic process but the significance of modified LDL in pathogenesis of vascular complications is unclear. We aimed to assess plasma modified LDL levels and their relationship with vascular function in African American diabetic patients. Methods: 125 patients with type-2 diabetes were enrolled. Levels of glycated LDL (g-LDL), carbamylated LDL (c-LDL), nitrated LDL (n-LDL) and oxidized LDL (ox-LDL) were quantified by ELISA. Microvascular function was assessed by vascular reactivity index (VRI), which assesses changes in digital temperature before and after release of arterial cuff occlusion. Carotid-femoral pulse wave velocity (PWV) assessed arterial stiffness using applanation tonometry. B-mode ultrasound image analysis assessed Carotid intima-media thickness (CIMT). Patient population was divided into well-controlled: HbA1c ≤7.0%, N=54; poorly-controlled: HbA1c >...
Diabetes, 2018
Previous studies have shown that HDL isolated from human atherosclerotic lesions and the blood of... more Previous studies have shown that HDL isolated from human atherosclerotic lesions and the blood of patients with established coronary artery disease contains elevated levels of nitrated apolipoprotein AI (NT-apoAI). The significance of NT-apoAI in the pathogenesis of vascular diabetic complications is not well understood. We aimed to evaluate the relationship between plasma NT-apoAI and vascular function in African-Americans diabetic patients. 125 patients with type 2 diabetes were enrolled over a 6 months period. Levels of plasma NT-apoAI was quantified by enzyme-linked immunosorbent assays (ELISA). Microvascular function was assessed by vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (CIMT) was assessed by B-mode ultrasound image analysis. Patients population was divided in two groups (well-controlled: HbA1c ≤7.0%, N=54; poorly-controlled: HbA1c >7.0%, N=71). Mean age 60±8 years; 64...
Diabetes, 2018
Patients with type 2 diabetes mellitus exhibit non-enzymatic glycation of lipoproteins that are c... more Patients with type 2 diabetes mellitus exhibit non-enzymatic glycation of lipoproteins that are considered proatherogenic modification contributing to increased susceptibility of patients with diabetes to atherosclerosis. We postulated that glycated lipoproteins might be associated with vascular outcome. To explore this, we studied the relationship between glycated HDL (gHDL) and glycated LDL (gLDL) with vascular function in African American diabetic patients. Total of 146 diabetic were enrolled over a 6 month period. Levels of plasma gHDL and gLDL were measured by enzyme-linked immunosorbent assay (ELISA). Microvascular function was assessed by vascular reactivity index (VRI). Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV). Carotid intima-media thickness (C-IMT) was assessed by B-mode ultrasound image analysis. Mean patient age was 60±8 years, 64% were female. 80% had hypertension, 90% had dyslipidemia and 15% had chronic kidney disease. Mean HbA1c...
Diabetes, 2018
Background: Diabetes is a robust risk factor for cardiovascular events which is marked in African... more Background: Diabetes is a robust risk factor for cardiovascular events which is marked in African Americans for reasons that may be attributed to socioeconomic and biologic vulnerabilities. Both structural (arterial thickening) and functional (arterial stiffness) abnormalities of the vasculature are predictive of incidental clinical events. Study objective was to determine the risk factors and relationship between large artery stiffness and carotid intimal thickness in African Americans with type II diabetes. Methods: 125 patients with diabetes were recruited from medical clinics. Medical information was obtained via interview and electronic medical record review. Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV) using applanation tonometry. Carotid Intimal Medial Thickness (CIMT) was obtained using B-mode ultrasound image analysis of the common carotid artery. Statistical analysis was done using SPSS version 23. Results: Mean age 60±8 years, 64% femal...
Journal of the American College of Cardiology, 2018
Journal of the American College of Cardiology, 2019
Background: Human F11 receptor (F11R) is an important cell adhesion molecule implicated in inflam... more Background: Human F11 receptor (F11R) is an important cell adhesion molecule implicated in inflammatory thrombosis. F11R is associated with the presence and severity of cardiovascular disease. Endothelial dysfunction in diabetes has been attributed to lack of bioavailability of nitric oxide (NO), another key player of platelet adhesion and aggregation. Our objective was to examine the relationship between F11R and NO and their impact on vascular function in African Americans with diabetes. Methods: 146 patients with diabetes were recruited from medical clinics over 6-month period. Plasma F11R and total NO were measured by ELISA. Microvascular function was assessed by vascular reactivity index (VRI), which assess changes in digital temperature before and after release of arterial cuff occlusion. Large artery stiffness was assessed by carotid-femoral pulse wave velocity (PWV) using applanation tonometry. Patient population was categorized into two groups: good control (HbA1c≤7; N=85) and poor control (HbA1c>7.0; N=61). Results: Age 60±8 years, female 64%, hypertension 82%, dyslipidemia 83%, diabetes duration 10.2±7.6 years, HbA1C= 8.1±2.2%. In the total population, F11R was negatively correlated with NO (r=-0.341, p=0.0001) and VRI (r=-0.191, P=0.022), but not PWV (r=0.082, p=0.335). In poor control group, F11R was significantly correlated with NO (r=-0.397, p=0.0001) but not VRI (r=-0.193, p=0.079). Univariate analysis showed plasma F11R predicted NO availability (β=-0.273, p=0.001) but not VRI (r=-0.234, p=0.475). Multiple linear regression analysis showed that F11R was independently associated with total NO levels (β=-0.339, p=0.009) after adjustment for age, gender, weight, hypertension, stroke, dyslipidemia, smoking, HbA1c, duration of diabetes, total cholesterol, LDLc, HDLc, triglycerides, medications. Conclusion: Our findings demonstrated significant inverse association between F11R and NO levels but neither were significantly associated with microvascular dysfunction or arterial stiffness in this cohort, suggesting F11R and NO may not be reliable markers of early vascular changes in diabetes. Larger prospective studies are needed to clarify these findings.
Journal of the American College of Cardiology, 2018
American Journal of Medical Case Reports, Jan 23, 2014
Circulation, 2019
Background: Endothelial dysfunction in diabetes has been attributed to lack of nitric oxide (NO) ... more Background: Endothelial dysfunction in diabetes has been attributed to lack of nitric oxide (NO) bioavailability. Oxidized LDL (OxLDL) and NO play antagonist roles in endothelium dysfunction. OxLDL...
Rationale: Diabetes mellitus is a risk factor for large and small vessel disease and portends a d... more Rationale: Diabetes mellitus is a risk factor for large and small vessel disease and portends a disproportionate morbidity and mortality burden in African Americans. Diabetes is often accompanied b...
Journal of Translational Science
Journal of Translational Science