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Research paper thumbnail of Prevalence of human immunodeficiency virus and tuberculosis among homeless individuals

Immunopathologia Persa, Jun 26, 2017

Research paper thumbnail of Study of the Epidemiology and Use of Condoms in Vulnerable Women Referring to Damage Reduction Centers in Shiraz During 2015-2016

International Journal of Epidemiologic Research

Background and aims: Female sex workers (FSWs) play an essential role in the transmission of HIV ... more Background and aims: Female sex workers (FSWs) play an essential role in the transmission of HIV and are considered critical groups compared to the public groups of society and their customers. Therefore, this study was conducted to investigate the status of sexual intercourse among prostitutes in Shiraz in 2015-2016. Methods: This is a cross-sectional study conducted on 162 prostitutes referred to Shiraz transitional damage reduction centers and the information was gathered through a checklist. Results: The results showed that the mean age at the first vaginal intercourse was 16.57±3.65. In 62.3% of people, the main reason for prostitution was the need for money to help the family. It was also found that 39.5% of prostitutes had anal sex, and more than 95% of them did not use condoms during sex. There was also a statistically significant relationship (P<0.01) between the age of sexual intercourse and education level. Conclusion: The results of this study demonstrated that prosti...

Research paper thumbnail of Animal Models of Human Artificial Ovary, Valuable Tools for Fertility Preservation in Cancer Patients

Research paper thumbnail of A study of the relationship between tuberculosis disease and the severity of COVID-19; a systematic review study

Journal of Preventive Epidemiology, 2021

Introduction: Studies on COVID-19 disease in patients with tuberculosis (TB) have not yet been wi... more Introduction: Studies on COVID-19 disease in patients with tuberculosis (TB) have not yet been widely performed and clear results have not been presented. It was found that; COVID-19 infection in TB patients quickly leads to disease and causes death in a small number of them. However, the prognosis of co-infection has not been evaluated in long run, since the association between TB and covid-19 cannot be ruled out, and therefore appropriate precautions and health system preparation are needed to deal with the impending burden of co-infection. The aim of this study was to provide a clear picture of the effects of these two diseases with each other. Methods: This study is considered as a systematic review of secondary studies. The statistical population is the studies that have been conducted in relation to the above topic and in the world. These articles were extracted from the databases of PubMed, Magiran, Science Direct, and Google Scholar. Out of 180 articles, 52 articles were rel...

Research paper thumbnail of Investigating the relationship between dietary acid load and risk of diabetes; a meta-analysis

Journal of Preventive Epidemiology, 2021

Introduction: Diabetes is the most common endocrine disease that annually causes four million dea... more Introduction: Diabetes is the most common endocrine disease that annually causes four million deaths in the world. It is not just a disease, however a series of metabolic disorders caused by defects in insulin secretion, insulin action, or both. Considering the importance of diet in the incidence and complications of diabetes, this study aimed to investigate the relationship between the dietary acid load with diabetes, which has recently been considered. Methods: This meta-analysis was first performed as a systematic review by searching in Google Scholar, Science Direct, PubMed, Wiley, Directory of Open Access Journals (DOAJ), Springer, Cochrane, ProQuest, and Scopus, as well as domestic databases including Magiran, SID, Irandoc, and Google Scholar. A total of 1511 articles were found that after reviewing and excluding irrelevant articles, five were included in the study. Results: In the present study, a significantly high heterogeneity was observed (I2 = 61.5, P = 0.01). A random e...

Research paper thumbnail of Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Considering the effects of epigenetic changes on the embryonic development through altering the g... more Considering the effects of epigenetic changes on the embryonic development through altering the gene expression of pluripotency, trophectoderm, and imprinting genes, we determined the pattern of epigenetic alterations in transgenic embryos produced by injecting transgenic mESCs into the morula. To aim this, RAG1 knock-out mESCs were produced using CRISPR-Cas9 editing system and then microinjected into morulas to develop chimeric embryos. Afterward, the expression of pluripotency, trophectoderm genes, and imprinting genes in these embryos were evaluated using real-time PCR. Immunohistochemical analysis was carried out to determine the methylation rates of H3K9me3 and H3K4me3. In the following, since epigenetic alterations can be at least one of the possible reasons of male infertility and the loss of germ-line transmission capacity, the germ-line transmission ability in chimeric mice was also evaluated by breeding them and subsequent backcrosses to wild type strains. Our findings sho...

Research paper thumbnail of Development of sheep primordial follicles encapsulated in alginate or in ovarian tissue in fresh and vitrified samples

Cryobiology, Jan 8, 2016

In vitro follicle growth is a promising strategy for female fertility preservation. This study wa... more In vitro follicle growth is a promising strategy for female fertility preservation. This study was conducted to compare the development of ovine follicles either isolated or in the context of ovarian cortical pieces after short term (8 days) three-dimensional culture in fresh and vitrified samples. Four different experiments were conducted; I) culture of ovarian cortical pieces encapsulated in 0.5% and 1% alginate and without alginate encapsulation (CP-0.5%, CP-1% and CP, respectively), II) culture of isolated primordial and primary follicles encapsulated in 1% and 2% alginate (IF-1% and IF-2%, respectively), III) culture of fresh and vitrified-warmed cortical pieces (F-CP and Vit-CP, respectively), and IV) culture of fresh and vitrified-warmed encapsulated isolated follicles (F-IF and Vit-IF, respectively). The number of secondary follicles after culture was negatively influenced by encapsulation of ovarian cortical pieces (6.3 ± 3.3 and 10.6 ± 0.9 vs 21.5 ± 2.3 in CP-0.5% and CP-1...

Research paper thumbnail of Transplantation of Goat Spermatogonial Stem Cells into the Mouse Rete Testis

Research paper thumbnail of Enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating

Avicenna journal of medical biotechnology, 2014

The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). ... more The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). They are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. The aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type A spermatogonia in dissociated cellular suspension of goat testes. Additionally, we evaluated the separated fractions of the gradients in percoll and samples in differential plating at different times for cell number, viability and purification rate of goat SSCs in culture. Testicular cells were successfully isolated from one month old goat testis using two-step enzymatic digestion and followed by two purification protocols, differential plating with different times of culture (3, 4, 5, and 6 hr) and discontinuous percoll density with different gradients (20, 28, 30, and 32%). The difference of percentage of undifferentiated SS...

Research paper thumbnail of The effect of aldosterone on Na+/K+/ATPase expression and development of embryos derived from vitrified-warmed sheep oocytes

Small Ruminant Research, 2015

Research paper thumbnail of Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Journal of Assisted Reproduction and Genetics, 2014

Research paper thumbnail of Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

Journal of Assisted Reproduction and Genetics, 2012

Research paper thumbnail of Platelet-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage

Reproductive biomedicine online, Jan 15, 2017

This study aimed to assess the effects of platelet-rich plasma (PRP) on growth and survival of is... more This study aimed to assess the effects of platelet-rich plasma (PRP) on growth and survival of isolated early human follicles in a three-dimensional culture system. After fresh and vitrified-warmed ovarian tissue was digested, isolated early preantral follicles and ovarian cells were separately encapsulated in 1% alginate (w/v). The encapsulated follicles and ovarian cells were cultured together in a medium supplemented with foetal bovine serum (FBS), PRP, PRP + FBS, or human serum albumin (HSA) for 10 days. Growth and survival of the follicles were assessed by measurement of diameter and staining with trypan blue. Follicular integrity was assessed by histological analysis. After culturing, all follicles increased in size, but growth rate was greater in follicles isolated from fresh samples than those from vitrified-warmed ones (P < 0.001). Similarly, follicular viability of fresh samples after culturing was higher than that of vitrified-warmed ones. The growth and survival rates...

Research paper thumbnail of Cultural and interpersonal risk factors for suicide ideation and suicide attempts among Muslim college students from 11 nations

Journal of Affective Disorders, 2021

BACKGROUND Research on suicidality in low to middle-income countries is scarce. We addressed this... more BACKGROUND Research on suicidality in low to middle-income countries is scarce. We addressed this issue by investigating suicidality in a cross-national college student samples from 11 predominantly low to middle-income majority Muslim countries. METHODS The sample consisted of 7427 college students (56% female) who reported to be affiliated with Islam. Data on self-construal, social support, negative life-events, acceptability of suicide, suicidal ideation, and suicide attempts were collected with self-administered questionnaires. RESULTS Acceptability of suicide and the experience of negative life-events were positively, and perceived social support was negatively associated with suicidal ideation. Interdependent self-construal was negatively related to the acceptability of suicide and positively associated with perceived social support, implying a negative indirect effect on suicidal ideation although its direct effect was positive. The number of negative life-events was the strongest positive predictor of ever attempting suicide. The interdependent self-construal moderated the association of negative life-events with suicide attempts. LIMITATIONS Cross-sectional and self-report nature of the study were its major limitations. Participants may have suppressed their responses about suicide because of religious and legal reasons. CONCLUSION Remarkable similarities across 11 country samples emerged in the linkages between cultural and interpersonal factors with suicidality. Our findings highlight the value of a nuanced approach to suicidality, that can recognize the differences in the processes associated with suicidal ideation and suicide attempts, as well as the need to consider the interplay of a broad range of personal, interpersonal, and cultural influences.

Research paper thumbnail of Prevalence of human immunodeficiency virus and tuberculosis among homeless individuals

Immunopathologia Persa, Jun 26, 2017

Research paper thumbnail of Study of the Epidemiology and Use of Condoms in Vulnerable Women Referring to Damage Reduction Centers in Shiraz During 2015-2016

International Journal of Epidemiologic Research

Background and aims: Female sex workers (FSWs) play an essential role in the transmission of HIV ... more Background and aims: Female sex workers (FSWs) play an essential role in the transmission of HIV and are considered critical groups compared to the public groups of society and their customers. Therefore, this study was conducted to investigate the status of sexual intercourse among prostitutes in Shiraz in 2015-2016. Methods: This is a cross-sectional study conducted on 162 prostitutes referred to Shiraz transitional damage reduction centers and the information was gathered through a checklist. Results: The results showed that the mean age at the first vaginal intercourse was 16.57±3.65. In 62.3% of people, the main reason for prostitution was the need for money to help the family. It was also found that 39.5% of prostitutes had anal sex, and more than 95% of them did not use condoms during sex. There was also a statistically significant relationship (P<0.01) between the age of sexual intercourse and education level. Conclusion: The results of this study demonstrated that prosti...

Research paper thumbnail of Animal Models of Human Artificial Ovary, Valuable Tools for Fertility Preservation in Cancer Patients

Research paper thumbnail of A study of the relationship between tuberculosis disease and the severity of COVID-19; a systematic review study

Journal of Preventive Epidemiology, 2021

Introduction: Studies on COVID-19 disease in patients with tuberculosis (TB) have not yet been wi... more Introduction: Studies on COVID-19 disease in patients with tuberculosis (TB) have not yet been widely performed and clear results have not been presented. It was found that; COVID-19 infection in TB patients quickly leads to disease and causes death in a small number of them. However, the prognosis of co-infection has not been evaluated in long run, since the association between TB and covid-19 cannot be ruled out, and therefore appropriate precautions and health system preparation are needed to deal with the impending burden of co-infection. The aim of this study was to provide a clear picture of the effects of these two diseases with each other. Methods: This study is considered as a systematic review of secondary studies. The statistical population is the studies that have been conducted in relation to the above topic and in the world. These articles were extracted from the databases of PubMed, Magiran, Science Direct, and Google Scholar. Out of 180 articles, 52 articles were rel...

Research paper thumbnail of Investigating the relationship between dietary acid load and risk of diabetes; a meta-analysis

Journal of Preventive Epidemiology, 2021

Introduction: Diabetes is the most common endocrine disease that annually causes four million dea... more Introduction: Diabetes is the most common endocrine disease that annually causes four million deaths in the world. It is not just a disease, however a series of metabolic disorders caused by defects in insulin secretion, insulin action, or both. Considering the importance of diet in the incidence and complications of diabetes, this study aimed to investigate the relationship between the dietary acid load with diabetes, which has recently been considered. Methods: This meta-analysis was first performed as a systematic review by searching in Google Scholar, Science Direct, PubMed, Wiley, Directory of Open Access Journals (DOAJ), Springer, Cochrane, ProQuest, and Scopus, as well as domestic databases including Magiran, SID, Irandoc, and Google Scholar. A total of 1511 articles were found that after reviewing and excluding irrelevant articles, five were included in the study. Results: In the present study, a significantly high heterogeneity was observed (I2 = 61.5, P = 0.01). A random e...

Research paper thumbnail of Epigenetic Events and Germ Line Transmission in Rag1 knock-out Chimeric Mice Produced by CRISPR/Cas9 Technology

Considering the effects of epigenetic changes on the embryonic development through altering the g... more Considering the effects of epigenetic changes on the embryonic development through altering the gene expression of pluripotency, trophectoderm, and imprinting genes, we determined the pattern of epigenetic alterations in transgenic embryos produced by injecting transgenic mESCs into the morula. To aim this, RAG1 knock-out mESCs were produced using CRISPR-Cas9 editing system and then microinjected into morulas to develop chimeric embryos. Afterward, the expression of pluripotency, trophectoderm genes, and imprinting genes in these embryos were evaluated using real-time PCR. Immunohistochemical analysis was carried out to determine the methylation rates of H3K9me3 and H3K4me3. In the following, since epigenetic alterations can be at least one of the possible reasons of male infertility and the loss of germ-line transmission capacity, the germ-line transmission ability in chimeric mice was also evaluated by breeding them and subsequent backcrosses to wild type strains. Our findings sho...

Research paper thumbnail of Development of sheep primordial follicles encapsulated in alginate or in ovarian tissue in fresh and vitrified samples

Cryobiology, Jan 8, 2016

In vitro follicle growth is a promising strategy for female fertility preservation. This study wa... more In vitro follicle growth is a promising strategy for female fertility preservation. This study was conducted to compare the development of ovine follicles either isolated or in the context of ovarian cortical pieces after short term (8 days) three-dimensional culture in fresh and vitrified samples. Four different experiments were conducted; I) culture of ovarian cortical pieces encapsulated in 0.5% and 1% alginate and without alginate encapsulation (CP-0.5%, CP-1% and CP, respectively), II) culture of isolated primordial and primary follicles encapsulated in 1% and 2% alginate (IF-1% and IF-2%, respectively), III) culture of fresh and vitrified-warmed cortical pieces (F-CP and Vit-CP, respectively), and IV) culture of fresh and vitrified-warmed encapsulated isolated follicles (F-IF and Vit-IF, respectively). The number of secondary follicles after culture was negatively influenced by encapsulation of ovarian cortical pieces (6.3 ± 3.3 and 10.6 ± 0.9 vs 21.5 ± 2.3 in CP-0.5% and CP-1...

Research paper thumbnail of Transplantation of Goat Spermatogonial Stem Cells into the Mouse Rete Testis

Research paper thumbnail of Enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating

Avicenna journal of medical biotechnology, 2014

The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). ... more The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). They are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. The aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type A spermatogonia in dissociated cellular suspension of goat testes. Additionally, we evaluated the separated fractions of the gradients in percoll and samples in differential plating at different times for cell number, viability and purification rate of goat SSCs in culture. Testicular cells were successfully isolated from one month old goat testis using two-step enzymatic digestion and followed by two purification protocols, differential plating with different times of culture (3, 4, 5, and 6 hr) and discontinuous percoll density with different gradients (20, 28, 30, and 32%). The difference of percentage of undifferentiated SS...

Research paper thumbnail of The effect of aldosterone on Na+/K+/ATPase expression and development of embryos derived from vitrified-warmed sheep oocytes

Small Ruminant Research, 2015

Research paper thumbnail of Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Journal of Assisted Reproduction and Genetics, 2014

Research paper thumbnail of Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

Journal of Assisted Reproduction and Genetics, 2012

Research paper thumbnail of Platelet-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage

Reproductive biomedicine online, Jan 15, 2017

This study aimed to assess the effects of platelet-rich plasma (PRP) on growth and survival of is... more This study aimed to assess the effects of platelet-rich plasma (PRP) on growth and survival of isolated early human follicles in a three-dimensional culture system. After fresh and vitrified-warmed ovarian tissue was digested, isolated early preantral follicles and ovarian cells were separately encapsulated in 1% alginate (w/v). The encapsulated follicles and ovarian cells were cultured together in a medium supplemented with foetal bovine serum (FBS), PRP, PRP + FBS, or human serum albumin (HSA) for 10 days. Growth and survival of the follicles were assessed by measurement of diameter and staining with trypan blue. Follicular integrity was assessed by histological analysis. After culturing, all follicles increased in size, but growth rate was greater in follicles isolated from fresh samples than those from vitrified-warmed ones (P < 0.001). Similarly, follicular viability of fresh samples after culturing was higher than that of vitrified-warmed ones. The growth and survival rates...

Research paper thumbnail of Cultural and interpersonal risk factors for suicide ideation and suicide attempts among Muslim college students from 11 nations

Journal of Affective Disorders, 2021

BACKGROUND Research on suicidality in low to middle-income countries is scarce. We addressed this... more BACKGROUND Research on suicidality in low to middle-income countries is scarce. We addressed this issue by investigating suicidality in a cross-national college student samples from 11 predominantly low to middle-income majority Muslim countries. METHODS The sample consisted of 7427 college students (56% female) who reported to be affiliated with Islam. Data on self-construal, social support, negative life-events, acceptability of suicide, suicidal ideation, and suicide attempts were collected with self-administered questionnaires. RESULTS Acceptability of suicide and the experience of negative life-events were positively, and perceived social support was negatively associated with suicidal ideation. Interdependent self-construal was negatively related to the acceptability of suicide and positively associated with perceived social support, implying a negative indirect effect on suicidal ideation although its direct effect was positive. The number of negative life-events was the strongest positive predictor of ever attempting suicide. The interdependent self-construal moderated the association of negative life-events with suicide attempts. LIMITATIONS Cross-sectional and self-report nature of the study were its major limitations. Participants may have suppressed their responses about suicide because of religious and legal reasons. CONCLUSION Remarkable similarities across 11 country samples emerged in the linkages between cultural and interpersonal factors with suicidality. Our findings highlight the value of a nuanced approach to suicidality, that can recognize the differences in the processes associated with suicidal ideation and suicide attempts, as well as the need to consider the interplay of a broad range of personal, interpersonal, and cultural influences.