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Papers by basavaraj chougule
PLOS ONE, Feb 20, 2013
<p>Flow cytometer CD Marker Expression for control hMSC (without Ag NPs).</p
Journal of Stem Cell Research & Therapy, 2013
a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,<... more a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,</p>d<p>Met: methionine,</p>e<p>Trp: tryptophan,</p>f<p>NA: Nucleic Acids.</p><p>A,U,G,T,C denote ring breathing modes of nucleic acid bases adenine, uracil, guanine, thymine, and cytosine.</p
<p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one ... more <p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one datum).</p
a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,<... more a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,</p>d<p>Met: methionine,</p>e<p>Trp: tryptophan,</p>f<p>NA: Nucleic Acids.</p><p>A,U,G,T,C denote ring breathing modes of nucleic acid bases adenine, uracil, guanine, thymine, and cytosine.</p
<p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one ... more <p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one datum).</p
<p>Magnification 4×.</p
<p>Magnification 4×.</p
<p>Magnification 4×.</p
<p>(a) Schematic representation of the Raman Tweezers set-up. (b) Picture of the Raman Twee... more <p>(a) Schematic representation of the Raman Tweezers set-up. (b) Picture of the Raman Tweezers set-up showing the geometry of the trapping laser beam and the Raman probe laser beam.</p
<p>The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum... more <p>The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum was measured using a laser power of ∼20 mW (785 nm wavelength); the acquisition time was 120 s and 5 accumulations were made.</p
PLOS ONE, 2013
<p>Flow cytometer CD Marker Expression for control hMSC (without Ag NPs).</p
<p>Top panel shows normalized Raman spectra of hMSCs exposed to different concentrations of... more <p>Top panel shows normalized Raman spectra of hMSCs exposed to different concentrations of Ag nano-particles (1–4 µg/mL). The normalization was with respect to the 999 cm<sup>−1</sup> peak. The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum was measured using a laser power of ∼20 mW (785 nm wavelength). Each spectrum is an average of spectra from five individual hMSCs. Acquisition time: 120 s and 5 accumulations. The lower panel shows the same spectra over an extended range (see text).</p
La presente invention concerne un procede d'isolement, de purification et d'expansion a l... more La presente invention concerne un procede d'isolement, de purification et d'expansion a l'echelle industrielle de cellules souches mesenchymateuses (MSC) derivees de tissu adipeux canin. L'invention concerne egalement un procede de traitement et un produit therapeutique pour traiter l'arthrose, une lesion de la moelle epiniere, la dermatite atopique, une cardiomyopathie dilatee, le diabete de type 1, l'insuffisance renale, une maladie hepatique et des plaies ne cicatrisant pas, comprenant des MSC.
We report here the application of Raman Tweezers system developed by our group for live, single c... more We report here the application of Raman Tweezers system developed by our group for live, single cell spectroscopy. Nanoparticle-induced biochemical changes in optically-trapped single cells have been monitored using the above set up. The study reveals that hMSCs treated with ZnO nanoparticles undergo oxidative stress at doping levels as low as 1 µg/Ml. This study suggests that there is strong concentration dependent oxidative stress induced by the ZnO nanoparticles in hMSCs.
Cytotherapy, 2012
Infections with a range of common community viruses remain a major cause of mortality and morbidi... more Infections with a range of common community viruses remain a major cause of mortality and morbidity after allogeneic hematopoietic stem cell transplantation. T cells specific for cytomegalovirus (CMV), Epstein-Barr virus (EBV) and adenoviruses can safely prevent and infections with these three most common culprits, but the manufacture of individual T cell lines for each virus would be prohibitive in terms of time and cost. We have demonstrated that T cells specific for all three viruses can be manufactured in a single culture using monocytes and EBVtransformed B lymphoblastoid cell lines (LCLs), both transduced with an adenovirus vector expressing pp65 of CMV, as antigen-presenting cells. Trivirus-specific T cell lines produced from healthy stem cell donors could prevent and treat infections with all three viruses, not only in the designated recipient, but in unrelated, partially-HLA-matched third party recipients. We now provide the details and logistics of T cell manufacture.
We report here results of a single-cell Raman spectroscopy study of stress effects induced by sil... more We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 mg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 mg/ml.
PLOS ONE, Feb 20, 2013
<p>Flow cytometer CD Marker Expression for control hMSC (without Ag NPs).</p
Journal of Stem Cell Research & Therapy, 2013
a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,<... more a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,</p>d<p>Met: methionine,</p>e<p>Trp: tryptophan,</p>f<p>NA: Nucleic Acids.</p><p>A,U,G,T,C denote ring breathing modes of nucleic acid bases adenine, uracil, guanine, thymine, and cytosine.</p
<p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one ... more <p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one datum).</p
a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,<... more a<p>p:protein,</p>b<p>Phe: phenylalanine,</p>c<p>Tyr: tyrosine,</p>d<p>Met: methionine,</p>e<p>Trp: tryptophan,</p>f<p>NA: Nucleic Acids.</p><p>A,U,G,T,C denote ring breathing modes of nucleic acid bases adenine, uracil, guanine, thymine, and cytosine.</p
<p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one ... more <p>Control, 1 µg/mL and 2 µg/mL NP treated cell spectra are clustered together (except one datum).</p
<p>Magnification 4×.</p
<p>Magnification 4×.</p
<p>Magnification 4×.</p
<p>(a) Schematic representation of the Raman Tweezers set-up. (b) Picture of the Raman Twee... more <p>(a) Schematic representation of the Raman Tweezers set-up. (b) Picture of the Raman Tweezers set-up showing the geometry of the trapping laser beam and the Raman probe laser beam.</p
<p>The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum... more <p>The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum was measured using a laser power of ∼20 mW (785 nm wavelength); the acquisition time was 120 s and 5 accumulations were made.</p
PLOS ONE, 2013
<p>Flow cytometer CD Marker Expression for control hMSC (without Ag NPs).</p
<p>Top panel shows normalized Raman spectra of hMSCs exposed to different concentrations of... more <p>Top panel shows normalized Raman spectra of hMSCs exposed to different concentrations of Ag nano-particles (1–4 µg/mL). The normalization was with respect to the 999 cm<sup>−1</sup> peak. The laser power used for trapping (1064 nm wavelength) was 5 mW while the Raman spectrum was measured using a laser power of ∼20 mW (785 nm wavelength). Each spectrum is an average of spectra from five individual hMSCs. Acquisition time: 120 s and 5 accumulations. The lower panel shows the same spectra over an extended range (see text).</p
La presente invention concerne un procede d'isolement, de purification et d'expansion a l... more La presente invention concerne un procede d'isolement, de purification et d'expansion a l'echelle industrielle de cellules souches mesenchymateuses (MSC) derivees de tissu adipeux canin. L'invention concerne egalement un procede de traitement et un produit therapeutique pour traiter l'arthrose, une lesion de la moelle epiniere, la dermatite atopique, une cardiomyopathie dilatee, le diabete de type 1, l'insuffisance renale, une maladie hepatique et des plaies ne cicatrisant pas, comprenant des MSC.
We report here the application of Raman Tweezers system developed by our group for live, single c... more We report here the application of Raman Tweezers system developed by our group for live, single cell spectroscopy. Nanoparticle-induced biochemical changes in optically-trapped single cells have been monitored using the above set up. The study reveals that hMSCs treated with ZnO nanoparticles undergo oxidative stress at doping levels as low as 1 µg/Ml. This study suggests that there is strong concentration dependent oxidative stress induced by the ZnO nanoparticles in hMSCs.
Cytotherapy, 2012
Infections with a range of common community viruses remain a major cause of mortality and morbidi... more Infections with a range of common community viruses remain a major cause of mortality and morbidity after allogeneic hematopoietic stem cell transplantation. T cells specific for cytomegalovirus (CMV), Epstein-Barr virus (EBV) and adenoviruses can safely prevent and infections with these three most common culprits, but the manufacture of individual T cell lines for each virus would be prohibitive in terms of time and cost. We have demonstrated that T cells specific for all three viruses can be manufactured in a single culture using monocytes and EBVtransformed B lymphoblastoid cell lines (LCLs), both transduced with an adenovirus vector expressing pp65 of CMV, as antigen-presenting cells. Trivirus-specific T cell lines produced from healthy stem cell donors could prevent and treat infections with all three viruses, not only in the designated recipient, but in unrelated, partially-HLA-matched third party recipients. We now provide the details and logistics of T cell manufacture.
We report here results of a single-cell Raman spectroscopy study of stress effects induced by sil... more We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 mg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 mg/ml.