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Kinetic analysis of human T-cell Leukemia virus ty by cecilia bender

Journal of Virology, 2009

Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia and is associa... more Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia and is associated with a variety of lymphocyte-mediated disorders. It has been hypothesized that a highly regulated pattern of HTLV-1 gene expression is critical for virus survival and disease pathogenesis. In this study, real-time reverse transcriptase PCR was used to determine the kinetics of viral gene expression in cells transiently transfected with an HTLV-1 proviral plasmid, in newly infected human peripheral blood mononuclear cells (PBMCs), and in PBMCs from newly infected rabbits. The HTLV-1 gene expression profiles in transiently transfected and infected cells were similar; over time, all transcripts increased and then maintained stable levels. gag/pol, tax/rex, and env mRNA were detected first and at the highest levels, whereas the expression levels of the accessory genes, including the antisense Hbz, were significantly lower than the tax/rex levels (ranging from 1 to 4 logs depending on the specific mRNA). In infected rabbits, tax/rex and gag/pol mRNA levels peaked early after inoculation and progressively decreased, which correlated inversely with the proviral load and host antibody response against viral proteins. Interestingly, Hbz mRNA was detectable at 1 week postinfection and increased and stabilized. The expression levels of all other HTLV-1 genes in infected rabbit PBMCs were at or below our limit of detection. This analysis provides insight into viral gene expression under various in vitro and in vivo experimental conditions. Our in vivo data indicate that in infected rabbits, Hbz mRNA expression over time directly correlates with the proviral load, which provides the first evidence linking Hbz expression to proviral load and the survival of the virus-infected cell in the host.

... AND PHOSPHORUS FERTILIZATION ON POST VITRO GROWTH OF MICROPROPAGATED Zingiber officinale ROSC... more ... AND PHOSPHORUS FERTILIZATION ON POST VITRO GROWTH OF MICROPROPAGATED Zingiber officinale ROSCOE (1) Rosilda dos Santos(2), Carla Giovana Girardi(3), Rosete Pescador(4) & Sidney Luiz Stürmer(5) SUMMARY ...

Nutrients, Jan 9, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antiox... more Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated forms like oleuropein and its aglycone. Still, its low dietary intake, poor bioavailability, and high inter-individual variability after absorption through the gastrointestinal tract hamper its full benefits.In a randomized, controlled, blind, cross-over study, we investigated the impact of HT metabolism and bioavailability by comparing two olive-derived watery supplements containing different doses of HT (30.58 and 61.48 mg of HT/dosage, respectively); additionally, HT-fortified olive oil was used in the control group. To this aim, plasma and urine samples were evaluated in 12 healthy volunteers following the intake of a single dose of the supplements or fortified olive oil. Blood and u...

Over the last decades the prevalent research on stevia has been focused on its steviol glycosides... more Over the last decades the prevalent research on stevia has been focused on its steviol glycosides. However, apart from the sweet glycosides, stevia leaves contain antioxidants, mainly chlorogenic acids but also flavonoids. Their antioxidant capacity might be useful for food preservation by preventing oxidative degradation. The antioxidant capacity can be measured in vitro by various assays that are presented in this chapter, of which only the oxygen radical absorbance capacity assay is standardised. Stevia leaves seem to be among the foods with the highest antioxidant capacity. Its antioxidant relevance for human health is questionable. For that, some evidence was obtained, mainly by bioassays, giving promising results. Nevertheless, further in vivo surveys are needed to substantiate the putative protective role of stevia against oxidative stress in humans. Stevia leaves gained attention due to their sweet taste caused by the steviol glycosides. Like all plants, stevia contains a va...

International Journal of Molecular Sciences

In the present study we report the efficacy of two food supplements derived from olives in reduci... more In the present study we report the efficacy of two food supplements derived from olives in reducing lipid oxidation. To this end, 12 healthy volunteers received a single dose (25 mL) of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), followed by an investigation of two reliable markers of oxidative stress. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 h post-intake. Plasma-oxidized low-density lipoprotein (oxLDL) cholesterol levels were measured with ELISA using a monoclonal antibody, while F2-isoprostanes (F2-IsoPs) were quantified in urine with UHPLC-DAD-MS/MS. Despite the great variability observed between individuals, a tendency to reduce lipoxidation reactions was observed in the blood in response to a single intake of the food supplements. In addition, the subgroup of individuals with the highest baseline oxLDL level showed a significant (p < 0.05) decrease in F2-IsoPs at 0.5 an...

In the present study we report the efficacy of food matrices derived from olives in preventing ox... more In the present study we report the efficacy of food matrices derived from olives in preventing oxidation of low-density lipoprotein cholesterol (LDL) and lipid peroxidation. To this end, 12 healthy volunteers were divided into 3 groups and cross-received a single dose of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), or as fortified extra virgin olive oil (12.19 mg HT + tyrosol). Blood and urine samples were collected at baseline and up to 12 hours after ingestion. Plasma oxidized LDL levels were measured by ELISA using a monoclonal antibody, while F2-isoprostanes were quantified in urine by UHPLC-MS/MS. Despite the great variability between individuals, a tendency to reduce lipoxidation reactions has been observed after olive phenolics intake in both, blood and urine. In addition, the subgroup of individuals with the highest baseline lipoxidation level showed a decrease in F2-isoprostanes (p < 0.05) after taking the foo...

This approach was used to first determine the steady-state levels of expression for the different... more This approach was used to first determine the steady-state levels of expression for the different viral transcripts in three different cell lines in log phase of growth (Fig. 3). Experiments performed indicated that gag/pol is the most abundant transcript. The expression level of env was comparable in the two T-cell lines, Mo-T and C344, infected by the 2A subtype, and was considerably higher than in the B-cells infected with HTLV-2B subtype, where p10/p11 and p? transcripts were below the limit of detection.

International Journal of Applied Sciences and Biotechnology, 2014

Objective: The aim of the present work was designed to study cellular antioxidant properties of s... more Objective: The aim of the present work was designed to study cellular antioxidant properties of selected plant extracts of Caralluma and Boucerosia on cell lines. Materials and Methods: The present study was carried out in four species of Caralluma R.Br. such as Caralluma adscendens (Roxb.) R. Brown var. attenuata (Wight) Grav. & Mayur. (CAA), Caralluma adscendens (Roxb.) R. Brown var. fimbriata (Wall.) Gravely & Mayur. (CAF), Caralluma stalagmifera C.E.C. Fisch. (CS) and Caralluma stalagmifera C.E.C. Fisch. var. longipetala Karupp. & Pull. (CSL) and as well as two species of Boucerosia Wight & Arn. such as Boucerosia lasiantha Wight. (BL) and Boucerosia umbellata (Haw.) Wight & Arn. (BU). The antioxidant efficacy was evaluated by ability of antioxidant molecules in the extract to scavenge ROS produced by normal metabolism by cells, observed by the oxidation of 2, 7- Dichloro fluorescein diacetate to dichlorofluorescein, followed by decrease in fluorescence intensity. Results: Cel...

Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal ... more Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal cancer progression has been inferred by the identification in tumour tissues of HCMV antigens and specific viral DNA or RNA sequences. To further investigate the relationship between HCMV and colorectal cancers we developed qualitative and quantitative PCR assay to detect HCMV DNA in 56 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients belonging to 4 different histological phenotypes: adenoma; poorly, moderately and well differentiated adenocarcinomas. Results: Of the 56 FFPE tested tissue samples, 6 (11%) were positive for HCMV nested PCR amplification, and more precisely 1 (5%) of 20 cases of adenoma and 5 (21%) of 24 cases of moderately differentiated adenocarcinoma. No PCR positivity was obtained in samples from well and poorly differentiated adenocarcinomas. Conclusion: Our observations suggest that there is no evidence of a direct association between HCMV and col...

This approach was used to first determine the steady-state levels of expression for the different... more This approach was used to first determine the steady-state levels of expression for the different viral transcripts in three different cell lines in log phase of growth (Fig. 3). Experiments performed indicated that gag/pol is the most abundant transcript. The expression level of env was comparable in the two T-cell lines, Mo-T and C344, infected by the 2A subtype, and was considerably higher than in the B-cells infected with HTLV-2B subtype, where p10/p11 and p? transcripts were below the limit of detection.

... CICLO XXII STUDY OF HUMAN T-LYMPHOTROPIC VIRUS TYPE 2 mRNA KINETICS OF EXPRESSION AND IDENTIF... more ... CICLO XXII STUDY OF HUMAN T-LYMPHOTROPIC VIRUS TYPE 2 mRNA KINETICS OF EXPRESSION AND IDENTIFICATION OF A NOVEL SPLICING SITE Dottoranda: CECILIABENDER Coordinatore: Prof.ssa Carla Voltattorni Tutor: Prof. Umberto Bertazzoni Page 2. ...

Several teas and herbal infusions are asserted as health promoters due to their content in antiox... more Several teas and herbal infusions are asserted as health promoters due to their content in antioxidant compounds. However the chemical methods of analysis to assess the antioxidants content do not give information regarding the biological activity of the studied substances. In the present study eight medicinal plants (3 C. Sinensis, R. Canina, Menta Piperita, S. Officinalis, I. Paraguariensis and C. Arabica, C. Robusta blend) as well as various antioxidant compounds naturally contained in these plants were screened to their antioxidant capacities, through chemical and cellular assays. Such medicinal plants were selected due to their wide utilization, both as infusions and as ingredients in cosmetics products and in food formulations. The main purpose was to assess their biological antioxidative capability measured in human cells, to assess their efficiency of protection against peroxyl radicals under physiological conditions, and later predict their in vivo activity. The plant extra...

Molecules

This work aimed to establish the synergic role of arbuscular mycorrhizal fungi (AMF) symbiosis, p... more This work aimed to establish the synergic role of arbuscular mycorrhizal fungi (AMF) symbiosis, phosphorus (P) fertilization and harvest time on the contents of stevia secondary metabolites. Consequently, steviol glycosides (SVglys) concentration and profile, total phenols and flavonoids as well as antioxidant assays, have been assessed in inoculated and no-inoculated plants, grown with or without P supply and collected at different growth stages(69, 89 and 123 days after transplanting).The obtained results suggest that the synthesis of stevia secondary metabolites is induced and/or modulated by all the investigated variability factors. In particular, AMF symbiosis promoted total SVglys content and positively influenced the concentration of some minor compounds (steviolbioside, dulcoside A and rebaudioside B), indicating a clear effect of mycorrhizal inoculation on SVglys biosynthetic pathway. Interestingly, only the mycorrhizal plants were able to synthesize rebaudioside B. In addi...

Applied Sciences

The present study evaluates the effect of olive (Olea europaea L.) vegetation water on human cell... more The present study evaluates the effect of olive (Olea europaea L.) vegetation water on human cells regarding its antioxidant properties and radical scavenger bioactivities. To this aim, two food supplements containing concentrated olive water in combination with 6% lemon juice or 70% grape juice, respectively, were assessed in different oxidation assays. From the investigated polyphenols, hydroxytyrosol, present in olives and in a lesser extent in grapes, was found to be the most abundant in both formulations, followed by tyrosol and oleuropein for the olive-derived concentrate with lemon juice, and by proanthocyanidins and tyrosol for the olive concentrate with grape juice. Cellular studies suggest that both formulations are effective antioxidants. In particular, the combination of olive and grape extracts showed a remarkable superoxides-, hydroxyl radicals-, and hydrogen peroxides-scavenging activity, while the formulation containing 94% olive concentrate wasmore potent in protect...

Beverages

The demand for antioxidant-rich beverages is steadily expanding. At the same time, the possibilit... more The demand for antioxidant-rich beverages is steadily expanding. At the same time, the possibility of manufacturing products with reduced sugar content, sweetened with natural ingredients, represents a decisive aspect of obtaining quality products with health benefits. Stevia rebaudiana Bertoni (stevia) is a natural sweetener that can help to control caloric intake and is also a good source of antioxidant compounds. The present study was designed to assess the feasibility of producing high-quality berry juices sweetened with dry-grinded stevia leaves or their crude extract. The effect of the stevia supplementation on the antioxidant activities of raspberry juices was determined at two different production steps by means of the Folin–Ciocalteu, the oxygen radical absorbance capacity (ORAC), and the cellular antioxidant activity (CAA) assays. The addition of stevia significantly increased the antioxidant activity of the juices and resulted in significantly higher ascorbic acid and tot...

Journal of Virology, 2009

Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia and is associa... more Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia and is associated with a variety of lymphocyte-mediated disorders. It has been hypothesized that a highly regulated pattern of HTLV-1 gene expression is critical for virus survival and disease pathogenesis. In this study, real-time reverse transcriptase PCR was used to determine the kinetics of viral gene expression in cells transiently transfected with an HTLV-1 proviral plasmid, in newly infected human peripheral blood mononuclear cells (PBMCs), and in PBMCs from newly infected rabbits. The HTLV-1 gene expression profiles in transiently transfected and infected cells were similar; over time, all transcripts increased and then maintained stable levels. gag/pol, tax/rex, and env mRNA were detected first and at the highest levels, whereas the expression levels of the accessory genes, including the antisense Hbz, were significantly lower than the tax/rex levels (ranging from 1 to 4 logs depending on the specific mRNA). In infected rabbits, tax/rex and gag/pol mRNA levels peaked early after inoculation and progressively decreased, which correlated inversely with the proviral load and host antibody response against viral proteins. Interestingly, Hbz mRNA was detectable at 1 week postinfection and increased and stabilized. The expression levels of all other HTLV-1 genes in infected rabbit PBMCs were at or below our limit of detection. This analysis provides insight into viral gene expression under various in vitro and in vivo experimental conditions. Our in vivo data indicate that in infected rabbits, Hbz mRNA expression over time directly correlates with the proviral load, which provides the first evidence linking Hbz expression to proviral load and the survival of the virus-infected cell in the host.

... AND PHOSPHORUS FERTILIZATION ON POST VITRO GROWTH OF MICROPROPAGATED Zingiber officinale ROSC... more ... AND PHOSPHORUS FERTILIZATION ON POST VITRO GROWTH OF MICROPROPAGATED Zingiber officinale ROSCOE (1) Rosilda dos Santos(2), Carla Giovana Girardi(3), Rosete Pescador(4) & Sidney Luiz Stürmer(5) SUMMARY ...

Nutrients, Jan 9, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antiox... more Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated forms like oleuropein and its aglycone. Still, its low dietary intake, poor bioavailability, and high inter-individual variability after absorption through the gastrointestinal tract hamper its full benefits.In a randomized, controlled, blind, cross-over study, we investigated the impact of HT metabolism and bioavailability by comparing two olive-derived watery supplements containing different doses of HT (30.58 and 61.48 mg of HT/dosage, respectively); additionally, HT-fortified olive oil was used in the control group. To this aim, plasma and urine samples were evaluated in 12 healthy volunteers following the intake of a single dose of the supplements or fortified olive oil. Blood and u...

Over the last decades the prevalent research on stevia has been focused on its steviol glycosides... more Over the last decades the prevalent research on stevia has been focused on its steviol glycosides. However, apart from the sweet glycosides, stevia leaves contain antioxidants, mainly chlorogenic acids but also flavonoids. Their antioxidant capacity might be useful for food preservation by preventing oxidative degradation. The antioxidant capacity can be measured in vitro by various assays that are presented in this chapter, of which only the oxygen radical absorbance capacity assay is standardised. Stevia leaves seem to be among the foods with the highest antioxidant capacity. Its antioxidant relevance for human health is questionable. For that, some evidence was obtained, mainly by bioassays, giving promising results. Nevertheless, further in vivo surveys are needed to substantiate the putative protective role of stevia against oxidative stress in humans. Stevia leaves gained attention due to their sweet taste caused by the steviol glycosides. Like all plants, stevia contains a va...

International Journal of Molecular Sciences

In the present study we report the efficacy of two food supplements derived from olives in reduci... more In the present study we report the efficacy of two food supplements derived from olives in reducing lipid oxidation. To this end, 12 healthy volunteers received a single dose (25 mL) of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), followed by an investigation of two reliable markers of oxidative stress. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 h post-intake. Plasma-oxidized low-density lipoprotein (oxLDL) cholesterol levels were measured with ELISA using a monoclonal antibody, while F2-isoprostanes (F2-IsoPs) were quantified in urine with UHPLC-DAD-MS/MS. Despite the great variability observed between individuals, a tendency to reduce lipoxidation reactions was observed in the blood in response to a single intake of the food supplements. In addition, the subgroup of individuals with the highest baseline oxLDL level showed a significant (p < 0.05) decrease in F2-IsoPs at 0.5 an...

In the present study we report the efficacy of food matrices derived from olives in preventing ox... more In the present study we report the efficacy of food matrices derived from olives in preventing oxidation of low-density lipoprotein cholesterol (LDL) and lipid peroxidation. To this end, 12 healthy volunteers were divided into 3 groups and cross-received a single dose of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), or as fortified extra virgin olive oil (12.19 mg HT + tyrosol). Blood and urine samples were collected at baseline and up to 12 hours after ingestion. Plasma oxidized LDL levels were measured by ELISA using a monoclonal antibody, while F2-isoprostanes were quantified in urine by UHPLC-MS/MS. Despite the great variability between individuals, a tendency to reduce lipoxidation reactions has been observed after olive phenolics intake in both, blood and urine. In addition, the subgroup of individuals with the highest baseline lipoxidation level showed a decrease in F2-isoprostanes (p < 0.05) after taking the foo...

This approach was used to first determine the steady-state levels of expression for the different... more This approach was used to first determine the steady-state levels of expression for the different viral transcripts in three different cell lines in log phase of growth (Fig. 3). Experiments performed indicated that gag/pol is the most abundant transcript. The expression level of env was comparable in the two T-cell lines, Mo-T and C344, infected by the 2A subtype, and was considerably higher than in the B-cells infected with HTLV-2B subtype, where p10/p11 and p? transcripts were below the limit of detection.

International Journal of Applied Sciences and Biotechnology, 2014

Objective: The aim of the present work was designed to study cellular antioxidant properties of s... more Objective: The aim of the present work was designed to study cellular antioxidant properties of selected plant extracts of Caralluma and Boucerosia on cell lines. Materials and Methods: The present study was carried out in four species of Caralluma R.Br. such as Caralluma adscendens (Roxb.) R. Brown var. attenuata (Wight) Grav. & Mayur. (CAA), Caralluma adscendens (Roxb.) R. Brown var. fimbriata (Wall.) Gravely & Mayur. (CAF), Caralluma stalagmifera C.E.C. Fisch. (CS) and Caralluma stalagmifera C.E.C. Fisch. var. longipetala Karupp. & Pull. (CSL) and as well as two species of Boucerosia Wight & Arn. such as Boucerosia lasiantha Wight. (BL) and Boucerosia umbellata (Haw.) Wight & Arn. (BU). The antioxidant efficacy was evaluated by ability of antioxidant molecules in the extract to scavenge ROS produced by normal metabolism by cells, observed by the oxidation of 2, 7- Dichloro fluorescein diacetate to dichlorofluorescein, followed by decrease in fluorescence intensity. Results: Cel...

Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal ... more Background: A possible association between human cytomegalovirus (HCMV) infection and colorectal cancer progression has been inferred by the identification in tumour tissues of HCMV antigens and specific viral DNA or RNA sequences. To further investigate the relationship between HCMV and colorectal cancers we developed qualitative and quantitative PCR assay to detect HCMV DNA in 56 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients belonging to 4 different histological phenotypes: adenoma; poorly, moderately and well differentiated adenocarcinomas. Results: Of the 56 FFPE tested tissue samples, 6 (11%) were positive for HCMV nested PCR amplification, and more precisely 1 (5%) of 20 cases of adenoma and 5 (21%) of 24 cases of moderately differentiated adenocarcinoma. No PCR positivity was obtained in samples from well and poorly differentiated adenocarcinomas. Conclusion: Our observations suggest that there is no evidence of a direct association between HCMV and col...

This approach was used to first determine the steady-state levels of expression for the different... more This approach was used to first determine the steady-state levels of expression for the different viral transcripts in three different cell lines in log phase of growth (Fig. 3). Experiments performed indicated that gag/pol is the most abundant transcript. The expression level of env was comparable in the two T-cell lines, Mo-T and C344, infected by the 2A subtype, and was considerably higher than in the B-cells infected with HTLV-2B subtype, where p10/p11 and p? transcripts were below the limit of detection.

... CICLO XXII STUDY OF HUMAN T-LYMPHOTROPIC VIRUS TYPE 2 mRNA KINETICS OF EXPRESSION AND IDENTIF... more ... CICLO XXII STUDY OF HUMAN T-LYMPHOTROPIC VIRUS TYPE 2 mRNA KINETICS OF EXPRESSION AND IDENTIFICATION OF A NOVEL SPLICING SITE Dottoranda: CECILIABENDER Coordinatore: Prof.ssa Carla Voltattorni Tutor: Prof. Umberto Bertazzoni Page 2. ...

Several teas and herbal infusions are asserted as health promoters due to their content in antiox... more Several teas and herbal infusions are asserted as health promoters due to their content in antioxidant compounds. However the chemical methods of analysis to assess the antioxidants content do not give information regarding the biological activity of the studied substances. In the present study eight medicinal plants (3 C. Sinensis, R. Canina, Menta Piperita, S. Officinalis, I. Paraguariensis and C. Arabica, C. Robusta blend) as well as various antioxidant compounds naturally contained in these plants were screened to their antioxidant capacities, through chemical and cellular assays. Such medicinal plants were selected due to their wide utilization, both as infusions and as ingredients in cosmetics products and in food formulations. The main purpose was to assess their biological antioxidative capability measured in human cells, to assess their efficiency of protection against peroxyl radicals under physiological conditions, and later predict their in vivo activity. The plant extra...

Molecules

This work aimed to establish the synergic role of arbuscular mycorrhizal fungi (AMF) symbiosis, p... more This work aimed to establish the synergic role of arbuscular mycorrhizal fungi (AMF) symbiosis, phosphorus (P) fertilization and harvest time on the contents of stevia secondary metabolites. Consequently, steviol glycosides (SVglys) concentration and profile, total phenols and flavonoids as well as antioxidant assays, have been assessed in inoculated and no-inoculated plants, grown with or without P supply and collected at different growth stages(69, 89 and 123 days after transplanting).The obtained results suggest that the synthesis of stevia secondary metabolites is induced and/or modulated by all the investigated variability factors. In particular, AMF symbiosis promoted total SVglys content and positively influenced the concentration of some minor compounds (steviolbioside, dulcoside A and rebaudioside B), indicating a clear effect of mycorrhizal inoculation on SVglys biosynthetic pathway. Interestingly, only the mycorrhizal plants were able to synthesize rebaudioside B. In addi...

Applied Sciences

The present study evaluates the effect of olive (Olea europaea L.) vegetation water on human cell... more The present study evaluates the effect of olive (Olea europaea L.) vegetation water on human cells regarding its antioxidant properties and radical scavenger bioactivities. To this aim, two food supplements containing concentrated olive water in combination with 6% lemon juice or 70% grape juice, respectively, were assessed in different oxidation assays. From the investigated polyphenols, hydroxytyrosol, present in olives and in a lesser extent in grapes, was found to be the most abundant in both formulations, followed by tyrosol and oleuropein for the olive-derived concentrate with lemon juice, and by proanthocyanidins and tyrosol for the olive concentrate with grape juice. Cellular studies suggest that both formulations are effective antioxidants. In particular, the combination of olive and grape extracts showed a remarkable superoxides-, hydroxyl radicals-, and hydrogen peroxides-scavenging activity, while the formulation containing 94% olive concentrate wasmore potent in protect...

Beverages

The demand for antioxidant-rich beverages is steadily expanding. At the same time, the possibilit... more The demand for antioxidant-rich beverages is steadily expanding. At the same time, the possibility of manufacturing products with reduced sugar content, sweetened with natural ingredients, represents a decisive aspect of obtaining quality products with health benefits. Stevia rebaudiana Bertoni (stevia) is a natural sweetener that can help to control caloric intake and is also a good source of antioxidant compounds. The present study was designed to assess the feasibility of producing high-quality berry juices sweetened with dry-grinded stevia leaves or their crude extract. The effect of the stevia supplementation on the antioxidant activities of raspberry juices was determined at two different production steps by means of the Folin–Ciocalteu, the oxygen radical absorbance capacity (ORAC), and the cellular antioxidant activity (CAA) assays. The addition of stevia significantly increased the antioxidant activity of the juices and resulted in significantly higher ascorbic acid and tot...