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Papers by yosra ben youssef

Medical mycology case reports, 2012

International journal of medical and pharmaceutical case reports, Jan 10, 2016

The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most commo... more The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most common cytogenetic abnormalities in acute myeloid leukemia (AML). It is associated with favorable prognosis. The t (5; 17) (q35; q21) is an uncommon translocation, fuses the gene for the nucleophosmin (NPM) to the retinoic acid receptor α(RARA) and was described essentially in acute promyelocytic leukemia (APL) variant. We present the case of a 19-year-old male patient who developed an AML with t (8 ;21) (q22 ;q22) associated to t(5 ;17)(q35 ;21). Morphology and immunophenotype of the leukemic cells were compatible with AML. The patient received chemotherapy based on cytarabine and anthracycline without all-trans retinoic acid (ATRA) followed by allogenic stem cells transplantation in first remission. To the best of our knowledge, this is the first report of an association between a rare translocation t (5; 17) and t (8;21) in AML. In this report, we will discuss the prognosis of this associa...

F1000Research

Background: Little data is actually available on sexual health problems with sexual activity, and... more Background: Little data is actually available on sexual health problems with sexual activity, and intimacy (sexual difficulty) in patients with hemophilia (PWH). We conducted this study to determine the prevalence of sexual difficulty in PWH and to determine factors associated with erectile dysfunction (ED). Methods: Based on The International Index of Erectile Function 15 (IIEF-15) questionnaire, we evaluated ED and other sexual problems in PWH. Results: Forty-Three (43) PWH were included in our study. The mean age was 33 years. Fourteen (32.6%) respondents were identified as having severe disease. The majority (93%) suffered from erectile dysfunction. Lack of desire and orgasm was observed in 76.7% of cases each. Lack of sexual satisfaction and global satisfaction were noted in 83.7% of cases and 88.4% of cases, respectively. Among PWH, older age was associated with ED. Conclusion: Our study illustrates the need for programs to assess and improve the sexual health of PWH in compre...

Pancreatic Cancer - Pathogenesis, Pathology, and Recent Advances in Diagnosis and Therapy [Working Title]

L-asparaginase (L-Aspa) is utilized as a part of the therapy in children with acute lymphoblastic... more L-asparaginase (L-Aspa) is utilized as a part of the therapy in children with acute lymphoblastic leukemia (ALL), achieving remission in 83–95% of the younger patients. Hypersensitivity reactions, as well as liver and pancreatic cytotoxicity, are severe documented side effects. L-Aspa-induced acute pancreatitis (AP) has been observed in 2.5–16% of treated patients. Patients with mild pancreatitis may be retreated with L-Aspa if they have no clinical symptoms within 48 hours, amylase and lipase levels are less than three times the normal’s upper limit, and there is no evidence of pseudocysts or necrosis on imaging. It is crucial to monitor patients under L-Aspa therapy, through careful observation of clinical signs and laboratory follow-up, as well as a continuous checkup for associated medications.

Revue de Médecine Interne, 2019

Introduction Le syndrome d’Evans (SE) est defini par la presence de deux ou plusieurs cytopenies ... more Introduction Le syndrome d’Evans (SE) est defini par la presence de deux ou plusieurs cytopenies auto-immunes survenant de facon simultanee ou sequentielle avec un delai d’apparition et une duree variable. Il s’agit souvent de l’association d’une anemie hemolytique (AHAI) et d’une thrombopenie immunologique (TIP) ou rarement d’une AHAI et d’une neutropenie auto-immune (NAI). Il s’agit d’une condition qui est le plus souvent idiopathique ou secondaire a un syndrome lymphoproliferatif ou une connectivite. Le but de notre travail est de decrire les principales caracteristiques cliniques, paracliniques, therapeutiques et les modalites evolutives des syndromes d’Evans. Patients et methodes Etude retrospective de 37 cas (2000–2018) concernant les patients hospitalises pour le bilan etiologique d’une AHAI et TIP. Les criteres d’inclusions : AHAI definie par une anemie ( Resultats Trente-sept patients ont ete colliges dont 24 femmes et 13 hommes. L’âge median au moment du diagnostic etait d...

Invasive pulmonary aspergillosis (IPA) remains a life threatening complication in immuno-compromi... more Invasive pulmonary aspergillosis (IPA) remains a life threatening complication in immuno-compromised and especially in neutropenic patients. We report our experience in the diagnosis and therapeutic management of IPA in 8 patients with acute leukemia. All patients were neutropenic (PNN< 100/mm3, mean duration = 37 days) when IPA was diagnosed. Clinical signs included fever above 39° and cough in all cases, chest pain in 4 cases, hemoptysis in 3 cases, rales in 5 cases. Chest x ray showed one lesion in 4 cases and multiple lesions in 4 cases. The diagnosis of IPA was established by bronchoalveolar lavage (BAL) in 5 cases, tissue biopsy in one case, positive sputum in one case and it was highly probable in one case. Thoracic computed tomographic (CT) scans were preformed after diagnosis confirmation of IPA and showed one or multiple lesions with air crescent signs.

Haematologica, 2010

15th Annual Meeting of the European-Hematology-Association, Barcelona, SPAIN, jun-13, 2010Interna... more 15th Annual Meeting of the European-Hematology-Association, Barcelona, SPAIN, jun-13, 2010International audienceno abstrac

Brazilian Journal of Medical and Biological Research, 2017

IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessm... more IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in BALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm BALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed BALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven BALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of BALL , introduced for the first time in Tunisian laboratories.

Revue Française d'Allergologie, 2017

Introduction Les effets indesirables de l’ondansetron sont generalement benins, toutefois des cho... more Introduction Les effets indesirables de l’ondansetron sont generalement benins, toutefois des chocs anaphylactiques ont ete rapportes. Methodes Nous rapportons un cas de choc anaphylactique induit par l’ondansetron notifie a notre service. Resultats Patiente âgee de 33 ans sans antecedent pathologique notable suivie pour une leucemie myeloblastique aigue traitee par chimiotherapie et de l’ondansetron pour prevenir les nausees et les vomissements. L’induction et la premiere cure de consolidation sont deroulees sans incident. Au cours de la perfusion de l’ondansetron durant la 2 e cure, la patiente se plaint de vertige, sueur froide et rapidement elle a developpe une hypotension (80/40 mmHg) et une bradycardie (45 bat/min). La perfusion a ete arretee et elle a recu des corticoides mais l’hypotension persiste necessitant des bolus d’adrenaline et une perfusion continue de la noradrenaline. L’evolution a ete favorable avec stabilite hemodynamique. La patiente a recu sa troisieme et sa derniere cure de chimiotherapie sans ondansetron. Des prick-tests cutanes etaient negatifs a l’ondansetron et l’intradermoreaction n’a pas ete pratiquee vue la gravite de la reaction. Discussion Le diagnostic retenu chez notre patiente est celui d’un choc anaphylactique a l’ondansetron, ce type de reaction quoique rare, a ete deja decrit. La cause de ce type de reaction est tres peu connue. Il apparait impossible de discerner un mecanisme anaphylactique (medie par les IgE), d’un mecanisme anaphylactoide, en rapport avec une histaminoliberation non specifique. La negativite des prick-tests cutanes comme c’est le cas chez notre patiente, plaide contre la possibilite d’un mecanisme IgE-dependant sans l’exclure formellement. Les medicaments utilises dans la chimiotherapie sont connus pour pouvoir provoquer eux aussi ce type de reaction mais l’evolution favorable malgre leur reutilisation durant la 3 e et la derniere cure va a l’encontre de leur responsabilite. Conclusion Les chocs anaphylactiques sous ondansetron pourraient se produire chez les patients qui ont precedemment recu le produit sans incident.

Archives de Pédiatrie, 2016

Journal de Mycologie Médicale / Journal of Medical Mycology, 2005

Geotrichum capitatum septicemias are rare, occurring exclusively in immunocompromised patients. W... more Geotrichum capitatum septicemias are rare, occurring exclusively in immunocompromised patients. We report a case of a 55-year-old man with acute myeloid leukemia who developed a G. capitatum septicemia with lung and skin lesions in the post chemotherapy aplasic phase. Treatment with amphotericin B (cumulative dose of 2 g) and maintenance with itraconazole resolved the clinical and radiological manifestations and controlled reactivation of the infection during the subsequent courses of cytotoxic chemotherapy. The emerging role of G. capitatum as an opportunistic agent in immunocompromised patient is stressed.

La Revue de Médecine Interne, 2012

Immuno-analyse & Biologie Spécialisée, 2013

Multiple myeloma (MM) is characterized by plasma cells proliferation within the bone marrow and a... more Multiple myeloma (MM) is characterized by plasma cells proliferation within the bone marrow and an excess of secreted monoclonal immunoglobulins. The authors report the results of an epidemiological, clinical, biological diagnosis and evolutive retrospective study including 54 cases of MM gathered in the hematology clinic at the CHU Farhat Hached Sousse during a three year period. The median age of the population is 67 years with sex-ratio: 1.7. Initial findings are bone pain (74.1% of patients), anemia syndrome (37%), and impaired general condition (31.5%). Serum protein electrophoresis showed a spike in 75.9%. Isotypes repartition is: IgG (61.8%), IgA (29.4%), monoclonal light chains (5.9%). A plasmocytosis more than 10% is detected in 76% of cases. Renal insufficiency is observed in 19% of cases. According to the Durie and Salmon classification, all of our patients are classified as stage III. Among other parameters, CRP and ␤2-microglobulin level are increased (≥ 6 mg/L) in respectively 36.2% and 28.6% of the cases. The cytogenetic abnormalities are detected in six cases. The median survival is 10 months.

La Revue de Médecine Interne, 2012

A154 66 e Congrès de la Société nationale franç aise de médecine interne-12 au 14 décembre 2012, ... more A154 66 e Congrès de la Société nationale franç aise de médecine interne-12 au 14 décembre 2012, Nice / La Revue de médecine interne 33S (2012) A90-A198

Medical Oncology, 2013

Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation bet... more Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation between chromosomes 15 and 17, t(15;17)(q22;q21), which results in the fusion gene PML-RARA. A small proportion of patients with APL have complex or simple variants of this translocation. With conventional cytogenetic methods, these translocations are detected in about 70-90 % of patients, with most of the negative results due to technical problems or cryptic variants. Those masked PML/RARA fusions can be identified by molecular analyses such as reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH). We report the case of a 58-year-old man showing morphological, cytochemical, and immunophenotypic features of hypergranular APL (FAB-M4). PML-RARA transcripts were not evident on RT-PCR. Although cytogenetic tests revealed the presence of an apparently balanced translocation t(15;17) (q24;q11) with an abnormal chromosome 12 that characterized a M3 leukemia. This karyotypic interpretation was confirmed by FISH with the use of painting probes of chromosomes 12, 15, and 17 and a PML-RARA dual-color DNA probe. FISH showed a PML-RARA fusion gene on the der(12) instead of the der(15). The patient was treated with an all-trans retinoic acid (ATRA) plus anthracycline-based protocol and achieved complete remission, with no recurrence to date. These results illustrate the usefulness of combining cytogenetics and FISH methods to evidence the PML/RARA fusion gene in cases with morphologic suspicion of APL with variant or cryptic t(15;17).

La Revue de Médecine Interne, 2013

La Revue de Médecine Interne, 2012

Medical mycology case reports, 2012

International journal of medical and pharmaceutical case reports, Jan 10, 2016

The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most commo... more The t (8; 21) (q22; q22) with the resulting RUNX1- RUNX1T1 rearrangement is one of the most common cytogenetic abnormalities in acute myeloid leukemia (AML). It is associated with favorable prognosis. The t (5; 17) (q35; q21) is an uncommon translocation, fuses the gene for the nucleophosmin (NPM) to the retinoic acid receptor α(RARA) and was described essentially in acute promyelocytic leukemia (APL) variant. We present the case of a 19-year-old male patient who developed an AML with t (8 ;21) (q22 ;q22) associated to t(5 ;17)(q35 ;21). Morphology and immunophenotype of the leukemic cells were compatible with AML. The patient received chemotherapy based on cytarabine and anthracycline without all-trans retinoic acid (ATRA) followed by allogenic stem cells transplantation in first remission. To the best of our knowledge, this is the first report of an association between a rare translocation t (5; 17) and t (8;21) in AML. In this report, we will discuss the prognosis of this associa...

F1000Research

Background: Little data is actually available on sexual health problems with sexual activity, and... more Background: Little data is actually available on sexual health problems with sexual activity, and intimacy (sexual difficulty) in patients with hemophilia (PWH). We conducted this study to determine the prevalence of sexual difficulty in PWH and to determine factors associated with erectile dysfunction (ED). Methods: Based on The International Index of Erectile Function 15 (IIEF-15) questionnaire, we evaluated ED and other sexual problems in PWH. Results: Forty-Three (43) PWH were included in our study. The mean age was 33 years. Fourteen (32.6%) respondents were identified as having severe disease. The majority (93%) suffered from erectile dysfunction. Lack of desire and orgasm was observed in 76.7% of cases each. Lack of sexual satisfaction and global satisfaction were noted in 83.7% of cases and 88.4% of cases, respectively. Among PWH, older age was associated with ED. Conclusion: Our study illustrates the need for programs to assess and improve the sexual health of PWH in compre...

Pancreatic Cancer - Pathogenesis, Pathology, and Recent Advances in Diagnosis and Therapy [Working Title]

L-asparaginase (L-Aspa) is utilized as a part of the therapy in children with acute lymphoblastic... more L-asparaginase (L-Aspa) is utilized as a part of the therapy in children with acute lymphoblastic leukemia (ALL), achieving remission in 83–95% of the younger patients. Hypersensitivity reactions, as well as liver and pancreatic cytotoxicity, are severe documented side effects. L-Aspa-induced acute pancreatitis (AP) has been observed in 2.5–16% of treated patients. Patients with mild pancreatitis may be retreated with L-Aspa if they have no clinical symptoms within 48 hours, amylase and lipase levels are less than three times the normal’s upper limit, and there is no evidence of pseudocysts or necrosis on imaging. It is crucial to monitor patients under L-Aspa therapy, through careful observation of clinical signs and laboratory follow-up, as well as a continuous checkup for associated medications.

Revue de Médecine Interne, 2019

Introduction Le syndrome d’Evans (SE) est defini par la presence de deux ou plusieurs cytopenies ... more Introduction Le syndrome d’Evans (SE) est defini par la presence de deux ou plusieurs cytopenies auto-immunes survenant de facon simultanee ou sequentielle avec un delai d’apparition et une duree variable. Il s’agit souvent de l’association d’une anemie hemolytique (AHAI) et d’une thrombopenie immunologique (TIP) ou rarement d’une AHAI et d’une neutropenie auto-immune (NAI). Il s’agit d’une condition qui est le plus souvent idiopathique ou secondaire a un syndrome lymphoproliferatif ou une connectivite. Le but de notre travail est de decrire les principales caracteristiques cliniques, paracliniques, therapeutiques et les modalites evolutives des syndromes d’Evans. Patients et methodes Etude retrospective de 37 cas (2000–2018) concernant les patients hospitalises pour le bilan etiologique d’une AHAI et TIP. Les criteres d’inclusions : AHAI definie par une anemie ( Resultats Trente-sept patients ont ete colliges dont 24 femmes et 13 hommes. L’âge median au moment du diagnostic etait d...

Invasive pulmonary aspergillosis (IPA) remains a life threatening complication in immuno-compromi... more Invasive pulmonary aspergillosis (IPA) remains a life threatening complication in immuno-compromised and especially in neutropenic patients. We report our experience in the diagnosis and therapeutic management of IPA in 8 patients with acute leukemia. All patients were neutropenic (PNN< 100/mm3, mean duration = 37 days) when IPA was diagnosed. Clinical signs included fever above 39° and cough in all cases, chest pain in 4 cases, hemoptysis in 3 cases, rales in 5 cases. Chest x ray showed one lesion in 4 cases and multiple lesions in 4 cases. The diagnosis of IPA was established by bronchoalveolar lavage (BAL) in 5 cases, tissue biopsy in one case, positive sputum in one case and it was highly probable in one case. Thoracic computed tomographic (CT) scans were preformed after diagnosis confirmation of IPA and showed one or multiple lesions with air crescent signs.

Haematologica, 2010

15th Annual Meeting of the European-Hematology-Association, Barcelona, SPAIN, jun-13, 2010Interna... more 15th Annual Meeting of the European-Hematology-Association, Barcelona, SPAIN, jun-13, 2010International audienceno abstrac

Brazilian Journal of Medical and Biological Research, 2017

IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessm... more IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in BALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm BALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed BALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven BALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of BALL , introduced for the first time in Tunisian laboratories.

Revue Française d'Allergologie, 2017

Introduction Les effets indesirables de l’ondansetron sont generalement benins, toutefois des cho... more Introduction Les effets indesirables de l’ondansetron sont generalement benins, toutefois des chocs anaphylactiques ont ete rapportes. Methodes Nous rapportons un cas de choc anaphylactique induit par l’ondansetron notifie a notre service. Resultats Patiente âgee de 33 ans sans antecedent pathologique notable suivie pour une leucemie myeloblastique aigue traitee par chimiotherapie et de l’ondansetron pour prevenir les nausees et les vomissements. L’induction et la premiere cure de consolidation sont deroulees sans incident. Au cours de la perfusion de l’ondansetron durant la 2 e cure, la patiente se plaint de vertige, sueur froide et rapidement elle a developpe une hypotension (80/40 mmHg) et une bradycardie (45 bat/min). La perfusion a ete arretee et elle a recu des corticoides mais l’hypotension persiste necessitant des bolus d’adrenaline et une perfusion continue de la noradrenaline. L’evolution a ete favorable avec stabilite hemodynamique. La patiente a recu sa troisieme et sa derniere cure de chimiotherapie sans ondansetron. Des prick-tests cutanes etaient negatifs a l’ondansetron et l’intradermoreaction n’a pas ete pratiquee vue la gravite de la reaction. Discussion Le diagnostic retenu chez notre patiente est celui d’un choc anaphylactique a l’ondansetron, ce type de reaction quoique rare, a ete deja decrit. La cause de ce type de reaction est tres peu connue. Il apparait impossible de discerner un mecanisme anaphylactique (medie par les IgE), d’un mecanisme anaphylactoide, en rapport avec une histaminoliberation non specifique. La negativite des prick-tests cutanes comme c’est le cas chez notre patiente, plaide contre la possibilite d’un mecanisme IgE-dependant sans l’exclure formellement. Les medicaments utilises dans la chimiotherapie sont connus pour pouvoir provoquer eux aussi ce type de reaction mais l’evolution favorable malgre leur reutilisation durant la 3 e et la derniere cure va a l’encontre de leur responsabilite. Conclusion Les chocs anaphylactiques sous ondansetron pourraient se produire chez les patients qui ont precedemment recu le produit sans incident.

Archives de Pédiatrie, 2016

Journal de Mycologie Médicale / Journal of Medical Mycology, 2005

Geotrichum capitatum septicemias are rare, occurring exclusively in immunocompromised patients. W... more Geotrichum capitatum septicemias are rare, occurring exclusively in immunocompromised patients. We report a case of a 55-year-old man with acute myeloid leukemia who developed a G. capitatum septicemia with lung and skin lesions in the post chemotherapy aplasic phase. Treatment with amphotericin B (cumulative dose of 2 g) and maintenance with itraconazole resolved the clinical and radiological manifestations and controlled reactivation of the infection during the subsequent courses of cytotoxic chemotherapy. The emerging role of G. capitatum as an opportunistic agent in immunocompromised patient is stressed.

La Revue de Médecine Interne, 2012

Immuno-analyse & Biologie Spécialisée, 2013

Multiple myeloma (MM) is characterized by plasma cells proliferation within the bone marrow and a... more Multiple myeloma (MM) is characterized by plasma cells proliferation within the bone marrow and an excess of secreted monoclonal immunoglobulins. The authors report the results of an epidemiological, clinical, biological diagnosis and evolutive retrospective study including 54 cases of MM gathered in the hematology clinic at the CHU Farhat Hached Sousse during a three year period. The median age of the population is 67 years with sex-ratio: 1.7. Initial findings are bone pain (74.1% of patients), anemia syndrome (37%), and impaired general condition (31.5%). Serum protein electrophoresis showed a spike in 75.9%. Isotypes repartition is: IgG (61.8%), IgA (29.4%), monoclonal light chains (5.9%). A plasmocytosis more than 10% is detected in 76% of cases. Renal insufficiency is observed in 19% of cases. According to the Durie and Salmon classification, all of our patients are classified as stage III. Among other parameters, CRP and ␤2-microglobulin level are increased (≥ 6 mg/L) in respectively 36.2% and 28.6% of the cases. The cytogenetic abnormalities are detected in six cases. The median survival is 10 months.

La Revue de Médecine Interne, 2012

A154 66 e Congrès de la Société nationale franç aise de médecine interne-12 au 14 décembre 2012, ... more A154 66 e Congrès de la Société nationale franç aise de médecine interne-12 au 14 décembre 2012, Nice / La Revue de médecine interne 33S (2012) A90-A198

Medical Oncology, 2013

Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation bet... more Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation between chromosomes 15 and 17, t(15;17)(q22;q21), which results in the fusion gene PML-RARA. A small proportion of patients with APL have complex or simple variants of this translocation. With conventional cytogenetic methods, these translocations are detected in about 70-90 % of patients, with most of the negative results due to technical problems or cryptic variants. Those masked PML/RARA fusions can be identified by molecular analyses such as reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH). We report the case of a 58-year-old man showing morphological, cytochemical, and immunophenotypic features of hypergranular APL (FAB-M4). PML-RARA transcripts were not evident on RT-PCR. Although cytogenetic tests revealed the presence of an apparently balanced translocation t(15;17) (q24;q11) with an abnormal chromosome 12 that characterized a M3 leukemia. This karyotypic interpretation was confirmed by FISH with the use of painting probes of chromosomes 12, 15, and 17 and a PML-RARA dual-color DNA probe. FISH showed a PML-RARA fusion gene on the der(12) instead of the der(15). The patient was treated with an all-trans retinoic acid (ATRA) plus anthracycline-based protocol and achieved complete remission, with no recurrence to date. These results illustrate the usefulness of combining cytogenetics and FISH methods to evidence the PML/RARA fusion gene in cases with morphologic suspicion of APL with variant or cryptic t(15;17).

La Revue de Médecine Interne, 2013

La Revue de Médecine Interne, 2012