b kolena - Academia.edu (original) (raw)
Papers by b kolena
Global NEST International Conference on Environmental Science & Technology
Consumer practices are considered to be the main source of human exposure to phthalates. The aim ... more Consumer practices are considered to be the main source of human exposure to phthalates. The aim of our study was biomonitoring of low molecular weight phthalate (LMWP) and high molecular weight phthalate (HMWP) metabolites in Slovak pre-school (n=100; age range 1-6 years) and school age children (n= 96; age range 7-15 years) and relate exposures to consumer practices. We used high performance liquid chromatography and tandem mass spectrometry for spot urine sample analysis to determine concentrations of 21 phthalate monoesters, metabolites of 11 LMWP and HMWP diesters. Median concentrations of ∑LMWP (177.32 µg.L⁻¹) were close to the ∑HMWP (169.83 µg.L⁻¹) metabolites. We observed significantly (p≤0.05) higher concentrations of 5 LMWP and 4 HMWP metabolites in school-age compared to preschool-age children. Data showed several statistically significant associations (p≤0.05) between concentrations of phthalate metabolites and the consumption of food and beverages. Interestingly, we obs...
Children
Phthalates alter the hormonal balance in humans during pregnancy, potentially affecting embryonic... more Phthalates alter the hormonal balance in humans during pregnancy, potentially affecting embryonic and fetal development. We studied the joint effect of exposure to phthalates, quantified by urinary phthalate metabolite concentration, and perceived psychological stress on the concentration of hormones in pregnant women (n = 90) from the Nitra region, Slovakia, up to the 15th week of pregnancy. We used high-performance liquid chromatography, tandem mass spectrometry (HPLC-MS/MS), and electro-chemiluminescence immunoassay to determine urinary concentrations of phthalates and serum concentrations of hormones, respectively. We used Cohen perceived stress scale (PSS) to evaluate the human perception of stressful situations. Our results showed that mono(carboxy-methyl-heptyl) phthalate (cx-MiNP) and a molar sum of di-iso-nonyl phthalate metabolites (ΣDiNP) were negatively associated with luteinizing hormone (LH) (p ≤ 0.05). Mono(hydroxy-methyl-octyl) phthalate (OH-MiNP) and the molar sum o...
Toxics
Information about the effects of phthalates and non-phthalate substitute cyclohexane-1,2-dicarbox... more Information about the effects of phthalates and non-phthalate substitute cyclohexane-1,2-dicarboxylic acid diisononyl ester (HEXAMOLL® DINCH) on children’s neurodevelopment is limited. The aim of the present research is to evaluate the association between phthalate/HEXAMOLL® DINCH exposure and child neurodevelopment in three European cohorts involved in HBM4EU Aligned Studies. Participating subjects were school-aged children belonging to the Northern Adriatic cohort II (NAC-II), Italy, Odense Child Cohort (OCC), Denmark, and PCB cohort, Slovakia. In each cohort, children’s neurodevelopment was assessed through the Full-Scale Intelligence Quotient score (FSIQ) of the Wechsler Intelligence Scale of Children test using three different editions. The children’s urine samples, collected for one point in time concurrently with the neurodevelopmental evaluation, were analyzed for several phthalates/HEXAMOLL® DINCH biomarkers. The relation between phthalates/HEXAMOLL® DINCH and FSIQ was expl...
Journal of Educational and Social Research, 2014
Background: Di(2-ethylhexyl) phthalate and di-n-butyl phthalate (DnBP) presents two of the most c... more Background: Di(2-ethylhexyl) phthalate and di-n-butyl phthalate (DnBP) presents two of the most commonly used phthalate plasticizers with a wide use mainly in production of PVC products, food packaging, medical devices, cosmetics and personal care products. Objectives: The aim of study was to assess exposure to phthalates based on the determination of urinary concentrations of mono(2-ethylhexyl) phthalate (MEHP)and monobutyl phthalate (MnBP) as primary metabolites of DEHP and DnBP in Roma children (n= 26; boys=12; girls= 14; average age 9.08 ± 3.57) in condition of Slovak republic. Methods: The urinary concentrations of MEHP and MnBP were determined by high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Results: The distribution of the MEHP levels ranged from 4.11 to 177.32 ng.ml-1 and from 56,41 to 628,52 ng.ml-1 in MnBP. The median value reached 14.53 ng.ml-1 in MEHP and 117.06 ng.ml-1 in MnBP. Statistically significant difference between the MEHP exc...
Mediterranean Journal of Social Sciences, 2013
The exposure of the general population to phthalates is of increasing public health concern. Most... more The exposure of the general population to phthalates is of increasing public health concern. Most substantial phthalate in the environment is di-(2-ethylhexyl) phthalate (DEHP); higher molecular weight is the reason for use in construction material, clothing and furnishings. By far, their largest application is to impart exibility to polyvinyl chloride plastic (PVC). Study focuses on the biomonitoring of DEHP in adults (n=108; males=42, females=66; average age 39.67 ± 14.01) from Nitra, because of the potential human toxicities of phthalates as a particle of plastic material. In response to determine human exposure to phthalates, we used high performance liquid chromatography and tandem mass spectrometry analysis to estimate trace levels phthalate metabolite mono-(2-ethylhexyl) phthalate (MEHP) in human urine. The mean daily intake for DEHP ranged from 4.66 to 141.58 μg/kg per day, in 18 subjects were higher than corresponding tolerable daily intake (TDI) for DEHP (50 μg/kg per day) set by the European Food Safety Authority. The presence of DEHP in more than 92% of the urine samples indicating a ubiquitous exposure of people living in Nitra. This study is the result of implementation of projects: "Environmental aspects of urban area" (ITMS: 26220220110) supported by the Research & Development Operational Programme funded by the ERDF; VEGA (V1/0042/12).
Academic Journal of Interdisciplinary Studies, 2013
The paper presents pathological findings of one individual from the St. Emmeram Cathedral, where ... more The paper presents pathological findings of one individual from the St. Emmeram Cathedral, where osteological remains were excavated during archaeological examination of the site Nitra-Castle hill, especially Nitra-Cathedral in 2008 and where bishops, canons and children (probably from aristocratic families) were buried since 14th till 18th century AD. The purpose of this study was determining pathological finding of skeletal remains of an individual from grave no. 235. Skeletal remains consist of right and left tibia, part of the left fibula (diaphysis with distal epiphysis) and foot bones, making ankylosis. Skeletal remains were anthropological analysed and sex of the individual was confirmed by aDNA analysis for increasing accuracy and reliability in sex determining. Presented osteological remains were anthropomorphological and paleopathological determined and diagnosed by RTG and CT scans to establish inflammatory disease of feet. After anthropological examination and widespread carefully study of osteo-archaeological and paleopathological literature we conclude on fact, that ankylosis was likely caused by rheumatoid arthritis (RA).
Human & Experimental Toxicology, 2016
We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressi... more We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressing apprentices by HPLC-MS/MS analyses and association with health status. Metabolites of mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono ethyl phthalate (MEP), mono- n-butyl phthalate (MnBP) and mono-iso-butyl phthalate (MiBP) were detected in all urine samples, followed by metabolites mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) and mono(2-etylhexyl) phthalate (MEHP) occurring in 97.06% and 86.76% of samples, respectively. Positive associations for females were observed between MnBP and fat-free mass index (FFMI) and age; negative associations were found between MEHP, MEOHP, MEHHP, sum of MEHP, MEHHP, MEOHP and vital capacity and also between MEHP and forced vital capacity (FVC of predicted value (PV)). Lengths of exposure were associated to MnBP, MEHHP, and MEP. We also documented positive associations between anthropometry (body mass index, waist-to-height ratio (WHtR), FFMI, fat m...
Global NEST International Conference on Environmental Science & Technology
Consumer practices are considered to be the main source of human exposure to phthalates. The aim ... more Consumer practices are considered to be the main source of human exposure to phthalates. The aim of our study was biomonitoring of low molecular weight phthalate (LMWP) and high molecular weight phthalate (HMWP) metabolites in Slovak pre-school (n=100; age range 1-6 years) and school age children (n= 96; age range 7-15 years) and relate exposures to consumer practices. We used high performance liquid chromatography and tandem mass spectrometry for spot urine sample analysis to determine concentrations of 21 phthalate monoesters, metabolites of 11 LMWP and HMWP diesters. Median concentrations of ∑LMWP (177.32 µg.L⁻¹) were close to the ∑HMWP (169.83 µg.L⁻¹) metabolites. We observed significantly (p≤0.05) higher concentrations of 5 LMWP and 4 HMWP metabolites in school-age compared to preschool-age children. Data showed several statistically significant associations (p≤0.05) between concentrations of phthalate metabolites and the consumption of food and beverages. Interestingly, we obs...
Children
Phthalates alter the hormonal balance in humans during pregnancy, potentially affecting embryonic... more Phthalates alter the hormonal balance in humans during pregnancy, potentially affecting embryonic and fetal development. We studied the joint effect of exposure to phthalates, quantified by urinary phthalate metabolite concentration, and perceived psychological stress on the concentration of hormones in pregnant women (n = 90) from the Nitra region, Slovakia, up to the 15th week of pregnancy. We used high-performance liquid chromatography, tandem mass spectrometry (HPLC-MS/MS), and electro-chemiluminescence immunoassay to determine urinary concentrations of phthalates and serum concentrations of hormones, respectively. We used Cohen perceived stress scale (PSS) to evaluate the human perception of stressful situations. Our results showed that mono(carboxy-methyl-heptyl) phthalate (cx-MiNP) and a molar sum of di-iso-nonyl phthalate metabolites (ΣDiNP) were negatively associated with luteinizing hormone (LH) (p ≤ 0.05). Mono(hydroxy-methyl-octyl) phthalate (OH-MiNP) and the molar sum o...
Toxics
Information about the effects of phthalates and non-phthalate substitute cyclohexane-1,2-dicarbox... more Information about the effects of phthalates and non-phthalate substitute cyclohexane-1,2-dicarboxylic acid diisononyl ester (HEXAMOLL® DINCH) on children’s neurodevelopment is limited. The aim of the present research is to evaluate the association between phthalate/HEXAMOLL® DINCH exposure and child neurodevelopment in three European cohorts involved in HBM4EU Aligned Studies. Participating subjects were school-aged children belonging to the Northern Adriatic cohort II (NAC-II), Italy, Odense Child Cohort (OCC), Denmark, and PCB cohort, Slovakia. In each cohort, children’s neurodevelopment was assessed through the Full-Scale Intelligence Quotient score (FSIQ) of the Wechsler Intelligence Scale of Children test using three different editions. The children’s urine samples, collected for one point in time concurrently with the neurodevelopmental evaluation, were analyzed for several phthalates/HEXAMOLL® DINCH biomarkers. The relation between phthalates/HEXAMOLL® DINCH and FSIQ was expl...
Journal of Educational and Social Research, 2014
Background: Di(2-ethylhexyl) phthalate and di-n-butyl phthalate (DnBP) presents two of the most c... more Background: Di(2-ethylhexyl) phthalate and di-n-butyl phthalate (DnBP) presents two of the most commonly used phthalate plasticizers with a wide use mainly in production of PVC products, food packaging, medical devices, cosmetics and personal care products. Objectives: The aim of study was to assess exposure to phthalates based on the determination of urinary concentrations of mono(2-ethylhexyl) phthalate (MEHP)and monobutyl phthalate (MnBP) as primary metabolites of DEHP and DnBP in Roma children (n= 26; boys=12; girls= 14; average age 9.08 ± 3.57) in condition of Slovak republic. Methods: The urinary concentrations of MEHP and MnBP were determined by high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Results: The distribution of the MEHP levels ranged from 4.11 to 177.32 ng.ml-1 and from 56,41 to 628,52 ng.ml-1 in MnBP. The median value reached 14.53 ng.ml-1 in MEHP and 117.06 ng.ml-1 in MnBP. Statistically significant difference between the MEHP exc...
Mediterranean Journal of Social Sciences, 2013
The exposure of the general population to phthalates is of increasing public health concern. Most... more The exposure of the general population to phthalates is of increasing public health concern. Most substantial phthalate in the environment is di-(2-ethylhexyl) phthalate (DEHP); higher molecular weight is the reason for use in construction material, clothing and furnishings. By far, their largest application is to impart exibility to polyvinyl chloride plastic (PVC). Study focuses on the biomonitoring of DEHP in adults (n=108; males=42, females=66; average age 39.67 ± 14.01) from Nitra, because of the potential human toxicities of phthalates as a particle of plastic material. In response to determine human exposure to phthalates, we used high performance liquid chromatography and tandem mass spectrometry analysis to estimate trace levels phthalate metabolite mono-(2-ethylhexyl) phthalate (MEHP) in human urine. The mean daily intake for DEHP ranged from 4.66 to 141.58 μg/kg per day, in 18 subjects were higher than corresponding tolerable daily intake (TDI) for DEHP (50 μg/kg per day) set by the European Food Safety Authority. The presence of DEHP in more than 92% of the urine samples indicating a ubiquitous exposure of people living in Nitra. This study is the result of implementation of projects: "Environmental aspects of urban area" (ITMS: 26220220110) supported by the Research & Development Operational Programme funded by the ERDF; VEGA (V1/0042/12).
Academic Journal of Interdisciplinary Studies, 2013
The paper presents pathological findings of one individual from the St. Emmeram Cathedral, where ... more The paper presents pathological findings of one individual from the St. Emmeram Cathedral, where osteological remains were excavated during archaeological examination of the site Nitra-Castle hill, especially Nitra-Cathedral in 2008 and where bishops, canons and children (probably from aristocratic families) were buried since 14th till 18th century AD. The purpose of this study was determining pathological finding of skeletal remains of an individual from grave no. 235. Skeletal remains consist of right and left tibia, part of the left fibula (diaphysis with distal epiphysis) and foot bones, making ankylosis. Skeletal remains were anthropological analysed and sex of the individual was confirmed by aDNA analysis for increasing accuracy and reliability in sex determining. Presented osteological remains were anthropomorphological and paleopathological determined and diagnosed by RTG and CT scans to establish inflammatory disease of feet. After anthropological examination and widespread carefully study of osteo-archaeological and paleopathological literature we conclude on fact, that ankylosis was likely caused by rheumatoid arthritis (RA).
Human & Experimental Toxicology, 2016
We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressi... more We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressing apprentices by HPLC-MS/MS analyses and association with health status. Metabolites of mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono ethyl phthalate (MEP), mono- n-butyl phthalate (MnBP) and mono-iso-butyl phthalate (MiBP) were detected in all urine samples, followed by metabolites mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) and mono(2-etylhexyl) phthalate (MEHP) occurring in 97.06% and 86.76% of samples, respectively. Positive associations for females were observed between MnBP and fat-free mass index (FFMI) and age; negative associations were found between MEHP, MEOHP, MEHHP, sum of MEHP, MEHHP, MEOHP and vital capacity and also between MEHP and forced vital capacity (FVC of predicted value (PV)). Lengths of exposure were associated to MnBP, MEHHP, and MEP. We also documented positive associations between anthropometry (body mass index, waist-to-height ratio (WHtR), FFMI, fat m...