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Papers by oya bulut

Bulgular: 167 adet (%82.6) kan serumu ve 160 adet (%79.2) süt serum örneği BVDV antikorları yönün... more Bulgular: 167 adet (%82.6) kan serumu ve 160 adet (%79.2) süt serum örneği BVDV antikorları yönünden pozitif belirlendi. Araştırmada 148 adet (%73.2) hayvandan alınan hem kan hem de süt serumu pozitif bulundu. 19 adet (%9.4) hayvanın sadece kan serumu BVDV antikor pozitif belirlenirken, 12 adet (%5.94) hayvanın ise sadece süt serumu antikor pozitif tespit edildi. İncelenen kan ve süt serum örneklerinde, BVDV antijen varlığı tespit edilmedi.

Pesquisa Veterinária Brasileira

ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes h... more ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.

Journal of Advances in VetBio Science and Techniques

In the present study, virological and serological investigations were performed to determine the ... more In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA while only one leukocyte sample (0.53%) was detected as antigen positive by IF. Seropositive and antigen identification results were considered as indication of infection. From the results of this study it can be concluded that CCoV infection is widespread in the Turkish dog population and the virus may be attributed to be one of the important viral agents in dogs. In conclusion diagnosis of CCoV is difficult because it can easily be mixed with respiratory, enteric and generalized infections by other viral, bacterial and parasitic agents, but diagnosis and the vaccine application are essential for the control and prevention of CCoV infections.

Journal of Animal and Veterinary Advances, Jul 1, 2012

Annual Research & Review in Biology

The aim of this study was to investigate the presence of BTV infection and possible vector specie... more The aim of this study was to investigate the presence of BTV infection and possible vector species in different regions of Turkey. In the study, blood samples taken from 666 Akkaraman sheep were examined. 2000 Culicoides specimens were captured by light traps from the same provinces and 20 Culicoides spp. were identified. Blood sera samples were investigated by c-ELISA and SNT for detecting Abs to BTV. Sera samples were detected as positive 67 (10.06%) and 160 (24.02%) by SNT and ELISA, respectively. SN 50 values of the 67 positive sera samples by SNT were detected between 1/2.38 and 1/200. All sheep blood samples and pools became Culicoides spp. samples were examined for BTV Ag presence by BTACE. Thirty six (5.40%) blood samples were detected as positive, but no from Culicoides pools. In the meantime, all sheep blood samples and Culicoides Original Research Article

Microbiology Research Journal International

Canine herpesvirus (CHV) seems to be present worldwide in both domestic and wild dogs. Serologic ... more Canine herpesvirus (CHV) seems to be present worldwide in both domestic and wild dogs. Serologic surveys have shown a relatively high prevalence of CHV in household and colony-bred dogs. Blood samples were collected from dogs in dog shelters or dog collection centres in Konya and Antalya that showed clinical signs of the disease. In total, 141 blood samples were examined for antibodies to CHV by commercial enzyme-linked immunosorbent assay, whereas the blood leukocyte samples obtained from the dogs were assessed by immunoflouresence test (IFT) for isolation of CHV. Madin-Darby canine kidney cells were used for virus isolation. Ninety-seven samples (68.8%) were detected as seropositive; while no positive results were identified using IFT. In conclusion, the high seropositivity may indicate that CHV infection is common in dog shelters or dog collection centres in Turkey and infected animals with CHV need to be identified and quarantined.

Journal of Istanbul Veterinary Sciences

Indian Journal of Animal Research, 2016

In the present study, virological and serological investigations were performed to determine the ... more In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA while only one leukocyte sample (0.53%) was detected as antigen positive by IF. Seropositive and antigen identification results were considered as indication of infection. From the results of this study it can be concluded that CCoV infection is widespread in the Turkish dog population and the virus may be attributed to be one of the important viral agents in dogs. In conclusion diagnosis of CCoV is difficult because it can easily be mixed with respiratory, enteric and generalized infections by other viral, bacterial and parasitic agents, but diagnosis and the vaccine application are essential for the control and prevention of CCoV infections.

Journal of Biotechnology, 2016

Ataturk Universitesi Veteriner Bilimleri Dergisi, 2013

Bovine Herpesvirus-1 (BHV-1)’in neden oldugu Infectious Bovine Rhinotracheitis (IBR) tum dunyada ... more Bovine Herpesvirus-1 (BHV-1)’in neden oldugu Infectious Bovine Rhinotracheitis (IBR) tum dunyada gorulmekte ve sigircilik endustrisinde ciddi ekonomik kayiplara neden olmaktadir. Bu calisma sigir kan serum orneklerinde BHV-1’e karsi gelisen antikor varliginin Enzyme-Linked Immunosorbent Assay (ELISA) ve mikro Notralizasyon Test (mNT)’i ile tespit edilmesi ve enfeksiyonun serolojik teshisinde kullanilan bu iki testin sensitivite ve spesifite degerlerinin belirlenmesi amaclari ile yapildi. Calismada Konya’daki ozel isletmelerden rastgele ornekleme ile temin edilen 301 adet sigir (BHV-1 yonunden asilanmamis) kan ornekleri kullanildi. Kan serum ornekleri BHV-1’e karsi olusan antikor varligi yonunden ELISA ve mNT ile incelendi. Titrasyon ve mNT testlerinde BHV-1’in ‘Colorado’ referens susu kullanildi. Virusun cogaltilmasi ve titresinin belirlenmesi icin Madin-Darby Bovine Kidney (MDBK) devamli hucre kulturleri kullanildi. BHV-1’e karsi olusan antikor varligi yonunden 26 adet (%8,6) kan serumu ornegi ELISA ile 22 (%7,3) adedi ise mNT ile seropozitif tespit edildi. Pozitif bulunan serumlarin Serum Notralizasyon 50 (SN50) degerleri 1/1,7 – 1/31,6 arasinda belirlendi. Sonuc olarak, elde edilen degerlere gore testlerin sensitivite ve spesifiteleri ile avantajlari karsilastirildi. ELISA icin sensitivite %100, spesifite %98 olarak belirlenirken; mNT icin sensitivite %84, spesifite ise %100 bulundu.

Acta Scientiae Veterinariae, 2014

Background: The bovine viral diarrhea virus (BVDV) causes mucosal lesions, respiratory disorders,... more Background: The bovine viral diarrhea virus (BVDV) causes mucosal lesions, respiratory disorders, spontaneous abortion, congenital abnormalities, and stillbirth in cattle and wild ruminant populations worldwide. Clinical categories of BVDV infection include persistent subclinical infection, acute transient infection, and mucosal disease. Virus neutralization, enzyme-linked immunosorbent assay (ELISA), and reverse transcription and polymerase chain reaction have been used for the detection of BVDV-infected cattle, but are time-consuming and costly methods, especially when screening large herds for persistent subclinical infections. In the current research, it was hypothesized that hemogram and blood gas values can be valuable indicator in the diagnosis and prognosis of infectious disease like metabolic disorders. The aim of this current study was to determine whether changes in the hematological parameters of BVDV-infected cattle represent potentially useful diagnostic factors. Materials, Methods & Results: Blood samples were collected from the jugular vein of 15 BVDV-antigen-positive (sick group) and 15 BVDV-antigen-negative (control group) Holstein cattle on a dairy farm in Konya Province in the Central Anatolia region of Turkey between January 2012 and September 2012. The presence of the BVDV antigen in the blood samples was determined with commercially available ELISA kit by using ELISA reader. Hemogram parameters [white blood cell counts (WBC), lymphocytes, monocytes, granulocytes, red blood cell counts (RBC), hematocrit (Hct), hemoglobin (Hb) and thrombocyte counts (THR)] obtained from anticoagulated bloods were measured with automatic cell counter. Blood gas values [power of hydrogen (pH), partial pressure of carbon dioxide (pCO 2), partial pressure of oxygen (pO 2), sodium (Na +2), potassium (K +), calcium (Ca +2), glucose (Glu), lactate (Lac), actual bicarbonate (HCO3act), standard bicarbonate (HCO 3 std), total carbon dioxide (tCO 2), base excess in vivo (BEvv), base excess in vitro (BEvt), oxygen content (sO 2)] were measured by blood gas analyzer. The data for the antigen-positive cattle were compared to those of the control cattle using independent-sample t-tests (SPSS 19.0 for windows). The results were expressed as Mean ± Standard Deviation. Difference were considered signifi cant when P <0.05. The white blood cell count, the relative proportions of lymphocytes and monocytes; the pO 2 and sO 2 values; and the serum levels of sodium and potassium in the BVDV-antigen-positive cattle were signifi cantly lower (P < 0.05) than those of the control cattle. By contrast, the relative proportion of granulocytes, the total CO 2 value, and the serum levels of actual bicarbonate and standard bicarbonate in the BVDV-antigen-positive cattle were signifi cantly higher (P < 0.05) than those of the control cattle. Discussion: Bovine viral diarrhea virus infection is a common viral disease in cattle and wild ruminant populations in the world. BVDV may result in mucosal, reproductive and respiratory disorders, abortions, mummifi cation, congenital anomalies, and still-births. Changes in the numbers of the various WBCs, blood gas values, and biochemical parameters might be useful diagnostic factors for the preliminary identifi cation of BVDV infection in cattle. Leukopenia and lymphopenia might also represent important prognosticators for BVDV-infected cattle because of the increased risk of secondary infections.

The Scientific World Journal, 2013

Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes i... more Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188-54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188-45.2%) blood samples were negative. Blood leukocyte samples from dogs were processed and inoculated onto confluent monolayers of MDCK cells using standard virological techniques. After third passage, cells were examined by direct immunoflourescence test for virus isolation. But positive result was not detected. In conclusion, this study clearly demonstrates the high prevalence of CAV infection in dogs.

Journal of Veterinary Medical Science, 2015

The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammat... more The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intraperitoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.

The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Jan 20, 2015

The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammat... more The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intra peritoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.

Eurasian Journal of Veterinary Sciences, 2014

The Scientific World Journal, 2013

Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes i... more Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188–54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188–45.2%) ...

Journal of Animal and Veterinary Advances, 2012

Journal of Animal and Veterinary Advances, 2012

Bulgular: 167 adet (%82.6) kan serumu ve 160 adet (%79.2) süt serum örneği BVDV antikorları yönün... more Bulgular: 167 adet (%82.6) kan serumu ve 160 adet (%79.2) süt serum örneği BVDV antikorları yönünden pozitif belirlendi. Araştırmada 148 adet (%73.2) hayvandan alınan hem kan hem de süt serumu pozitif bulundu. 19 adet (%9.4) hayvanın sadece kan serumu BVDV antikor pozitif belirlenirken, 12 adet (%5.94) hayvanın ise sadece süt serumu antikor pozitif tespit edildi. İncelenen kan ve süt serum örneklerinde, BVDV antijen varlığı tespit edilmedi.

Pesquisa Veterinária Brasileira

ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes h... more ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.

Journal of Advances in VetBio Science and Techniques

In the present study, virological and serological investigations were performed to determine the ... more In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA while only one leukocyte sample (0.53%) was detected as antigen positive by IF. Seropositive and antigen identification results were considered as indication of infection. From the results of this study it can be concluded that CCoV infection is widespread in the Turkish dog population and the virus may be attributed to be one of the important viral agents in dogs. In conclusion diagnosis of CCoV is difficult because it can easily be mixed with respiratory, enteric and generalized infections by other viral, bacterial and parasitic agents, but diagnosis and the vaccine application are essential for the control and prevention of CCoV infections.

Journal of Animal and Veterinary Advances, Jul 1, 2012

Annual Research & Review in Biology

The aim of this study was to investigate the presence of BTV infection and possible vector specie... more The aim of this study was to investigate the presence of BTV infection and possible vector species in different regions of Turkey. In the study, blood samples taken from 666 Akkaraman sheep were examined. 2000 Culicoides specimens were captured by light traps from the same provinces and 20 Culicoides spp. were identified. Blood sera samples were investigated by c-ELISA and SNT for detecting Abs to BTV. Sera samples were detected as positive 67 (10.06%) and 160 (24.02%) by SNT and ELISA, respectively. SN 50 values of the 67 positive sera samples by SNT were detected between 1/2.38 and 1/200. All sheep blood samples and pools became Culicoides spp. samples were examined for BTV Ag presence by BTACE. Thirty six (5.40%) blood samples were detected as positive, but no from Culicoides pools. In the meantime, all sheep blood samples and Culicoides Original Research Article

Microbiology Research Journal International

Canine herpesvirus (CHV) seems to be present worldwide in both domestic and wild dogs. Serologic ... more Canine herpesvirus (CHV) seems to be present worldwide in both domestic and wild dogs. Serologic surveys have shown a relatively high prevalence of CHV in household and colony-bred dogs. Blood samples were collected from dogs in dog shelters or dog collection centres in Konya and Antalya that showed clinical signs of the disease. In total, 141 blood samples were examined for antibodies to CHV by commercial enzyme-linked immunosorbent assay, whereas the blood leukocyte samples obtained from the dogs were assessed by immunoflouresence test (IFT) for isolation of CHV. Madin-Darby canine kidney cells were used for virus isolation. Ninety-seven samples (68.8%) were detected as seropositive; while no positive results were identified using IFT. In conclusion, the high seropositivity may indicate that CHV infection is common in dog shelters or dog collection centres in Turkey and infected animals with CHV need to be identified and quarantined.

Journal of Istanbul Veterinary Sciences

Indian Journal of Animal Research, 2016

In the present study, virological and serological investigations were performed to determine the ... more In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA while only one leukocyte sample (0.53%) was detected as antigen positive by IF. Seropositive and antigen identification results were considered as indication of infection. From the results of this study it can be concluded that CCoV infection is widespread in the Turkish dog population and the virus may be attributed to be one of the important viral agents in dogs. In conclusion diagnosis of CCoV is difficult because it can easily be mixed with respiratory, enteric and generalized infections by other viral, bacterial and parasitic agents, but diagnosis and the vaccine application are essential for the control and prevention of CCoV infections.

Journal of Biotechnology, 2016

Ataturk Universitesi Veteriner Bilimleri Dergisi, 2013

Bovine Herpesvirus-1 (BHV-1)’in neden oldugu Infectious Bovine Rhinotracheitis (IBR) tum dunyada ... more Bovine Herpesvirus-1 (BHV-1)’in neden oldugu Infectious Bovine Rhinotracheitis (IBR) tum dunyada gorulmekte ve sigircilik endustrisinde ciddi ekonomik kayiplara neden olmaktadir. Bu calisma sigir kan serum orneklerinde BHV-1’e karsi gelisen antikor varliginin Enzyme-Linked Immunosorbent Assay (ELISA) ve mikro Notralizasyon Test (mNT)’i ile tespit edilmesi ve enfeksiyonun serolojik teshisinde kullanilan bu iki testin sensitivite ve spesifite degerlerinin belirlenmesi amaclari ile yapildi. Calismada Konya’daki ozel isletmelerden rastgele ornekleme ile temin edilen 301 adet sigir (BHV-1 yonunden asilanmamis) kan ornekleri kullanildi. Kan serum ornekleri BHV-1’e karsi olusan antikor varligi yonunden ELISA ve mNT ile incelendi. Titrasyon ve mNT testlerinde BHV-1’in ‘Colorado’ referens susu kullanildi. Virusun cogaltilmasi ve titresinin belirlenmesi icin Madin-Darby Bovine Kidney (MDBK) devamli hucre kulturleri kullanildi. BHV-1’e karsi olusan antikor varligi yonunden 26 adet (%8,6) kan serumu ornegi ELISA ile 22 (%7,3) adedi ise mNT ile seropozitif tespit edildi. Pozitif bulunan serumlarin Serum Notralizasyon 50 (SN50) degerleri 1/1,7 – 1/31,6 arasinda belirlendi. Sonuc olarak, elde edilen degerlere gore testlerin sensitivite ve spesifiteleri ile avantajlari karsilastirildi. ELISA icin sensitivite %100, spesifite %98 olarak belirlenirken; mNT icin sensitivite %84, spesifite ise %100 bulundu.

Acta Scientiae Veterinariae, 2014

Background: The bovine viral diarrhea virus (BVDV) causes mucosal lesions, respiratory disorders,... more Background: The bovine viral diarrhea virus (BVDV) causes mucosal lesions, respiratory disorders, spontaneous abortion, congenital abnormalities, and stillbirth in cattle and wild ruminant populations worldwide. Clinical categories of BVDV infection include persistent subclinical infection, acute transient infection, and mucosal disease. Virus neutralization, enzyme-linked immunosorbent assay (ELISA), and reverse transcription and polymerase chain reaction have been used for the detection of BVDV-infected cattle, but are time-consuming and costly methods, especially when screening large herds for persistent subclinical infections. In the current research, it was hypothesized that hemogram and blood gas values can be valuable indicator in the diagnosis and prognosis of infectious disease like metabolic disorders. The aim of this current study was to determine whether changes in the hematological parameters of BVDV-infected cattle represent potentially useful diagnostic factors. Materials, Methods & Results: Blood samples were collected from the jugular vein of 15 BVDV-antigen-positive (sick group) and 15 BVDV-antigen-negative (control group) Holstein cattle on a dairy farm in Konya Province in the Central Anatolia region of Turkey between January 2012 and September 2012. The presence of the BVDV antigen in the blood samples was determined with commercially available ELISA kit by using ELISA reader. Hemogram parameters [white blood cell counts (WBC), lymphocytes, monocytes, granulocytes, red blood cell counts (RBC), hematocrit (Hct), hemoglobin (Hb) and thrombocyte counts (THR)] obtained from anticoagulated bloods were measured with automatic cell counter. Blood gas values [power of hydrogen (pH), partial pressure of carbon dioxide (pCO 2), partial pressure of oxygen (pO 2), sodium (Na +2), potassium (K +), calcium (Ca +2), glucose (Glu), lactate (Lac), actual bicarbonate (HCO3act), standard bicarbonate (HCO 3 std), total carbon dioxide (tCO 2), base excess in vivo (BEvv), base excess in vitro (BEvt), oxygen content (sO 2)] were measured by blood gas analyzer. The data for the antigen-positive cattle were compared to those of the control cattle using independent-sample t-tests (SPSS 19.0 for windows). The results were expressed as Mean ± Standard Deviation. Difference were considered signifi cant when P <0.05. The white blood cell count, the relative proportions of lymphocytes and monocytes; the pO 2 and sO 2 values; and the serum levels of sodium and potassium in the BVDV-antigen-positive cattle were signifi cantly lower (P < 0.05) than those of the control cattle. By contrast, the relative proportion of granulocytes, the total CO 2 value, and the serum levels of actual bicarbonate and standard bicarbonate in the BVDV-antigen-positive cattle were signifi cantly higher (P < 0.05) than those of the control cattle. Discussion: Bovine viral diarrhea virus infection is a common viral disease in cattle and wild ruminant populations in the world. BVDV may result in mucosal, reproductive and respiratory disorders, abortions, mummifi cation, congenital anomalies, and still-births. Changes in the numbers of the various WBCs, blood gas values, and biochemical parameters might be useful diagnostic factors for the preliminary identifi cation of BVDV infection in cattle. Leukopenia and lymphopenia might also represent important prognosticators for BVDV-infected cattle because of the increased risk of secondary infections.

The Scientific World Journal, 2013

Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes i... more Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188-54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188-45.2%) blood samples were negative. Blood leukocyte samples from dogs were processed and inoculated onto confluent monolayers of MDCK cells using standard virological techniques. After third passage, cells were examined by direct immunoflourescence test for virus isolation. But positive result was not detected. In conclusion, this study clearly demonstrates the high prevalence of CAV infection in dogs.

Journal of Veterinary Medical Science, 2015

The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammat... more The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intraperitoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.

The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Jan 20, 2015

The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammat... more The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intra peritoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.

Eurasian Journal of Veterinary Sciences, 2014

The Scientific World Journal, 2013

Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes i... more Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188–54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188–45.2%) ...

Journal of Animal and Veterinary Advances, 2012

Journal of Animal and Veterinary Advances, 2012