gaelle catanante - Academia.edu (original) (raw)

Papers by gaelle catanante

Research Square (Research Square), Sep 24, 2021

Analytical biochemistry, Jan 29, 2016

In this study, a simple TAMRA (tetramethyl-6-carboxyrhodamine) quenching based aptasenisng platfo... more In this study, a simple TAMRA (tetramethyl-6-carboxyrhodamine) quenching based aptasenisng platform was designed for the detection of aflatoxin B1 (AFB1). Herein, we compared the analytical performance of two aptamer sequences (seqA & seqB). The AFB1 detection was based on the interactions of FAM (carboxyfluorescein) labelled aptamer with TAMRA labelled DNA complementary strand in the presence and absence of target analyte. Under optimized experimental condition, TAMRA labelled strand quenched the fluorescence response of FAM labelled aptamer due to the non-covalent interaction between the two DNA strands. The binding of AFB1 induced the complex formation, weakened the interaction between FAM-labelled aptamer and TAMRA labelled complementary strand, resulting in the fluorescence recovery. By using this principle concept, an assay was constructed for the detection of AFB1. The method exhibited good sensitivity, selectivity with a limit of detection 0.2 ng mL(-1), and a wide linear ra...

Biosensors, 2022

Patulin contamination in fruits, vegetables, and their products is considered a serious health ri... more Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B1 (AFB1), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT d...

Antibiotics, 2020

In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (... more In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (OFL), being a principal quinolone commonly used in both human and animal healthcare. Since the affinity of a 72-mer ssDNA sequence has been previously described without further investigations, this paper demonstrates the first computational prediction of the binding motif between this aptamer and OFL through in silico molecular docking studies. Besides, we suggest the application of the characterized recognition mechanism in a simple FRET (Förster Resonance Energy Transfer) pattern for the rapid aptasensing of the quinolone of interest. Accordingly, our approach harnesses the fluorescence quenching of the fluorescein-tagged aptamer (FAM-APT) induced by its partial hybridization to a tetramethyl rhodamine-labelled complementary ssDNA (TAMRA-cDNA). In such a structure, dye labels brought into close proximity act as a FRET pair. Upon ofloxacin addition, an affinity competition occurs to for...

Food Chemistry, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Microchimica Acta, 2019

A method is described for electrochemical oxidation of polymers on the surface of screen-printed ... more A method is described for electrochemical oxidation of polymers on the surface of screen-printed electrodes (SPCE). These act as scaffold layers for homogeneous deposition of silver nanoparticles (AgNPs). Hexamethylenediamine (HMDA) and poly(ethylene glycol) were immobilized on the SPCE surface via electrochemical oxidation. AgNPs were then electrodeposited on the scaffolds on the SPCE. This type of different carbon chain containing materials like PEG and HMDA act as big tunnels for electron mobility and are useful for the homogenous deposition of AgNPs on the SPCE surface without agglomeration. The resulting sensor was applied to the determination of hydrogen peroxide (H2O2) as a model analyte. It is found to display favorable catalytic and conductive properties towards the reduction of H2O2. Cyclic voltammetry and amperometry revealed that the modified electrode performs better than other modified SPCEs. Best operated at a potential of around −0.61 V (vs Ag|AgCl), the amperometric response is linear in the 10–180 μM H2O2 concentration range and the detection limit is 1.5 μM. The sensor is stable and reproducible. The resultant sensor was appplied to toothpaste analysis, and good recovery values were gained. Graphical abstract Schematic representation of electropolymerization of poly(ethylene glycol) and hexamethylenediamine scaffold layers on screen-printed electrodes for homogeneous electrodeposition of silver nanoparticles. This electrode was applied for the amperometric determination of hydrogen peroxide. Schematic representation of electropolymerization of poly(ethylene glycol) and hexamethylenediamine scaffold layers on screen-printed electrodes for homogeneous electrodeposition of silver nanoparticles. This electrode was applied for the amperometric determination of hydrogen peroxide.

Analytical and Bioanalytical Chemistry, 2019

This work reports on further development of an optical biosensor for the in vitro detection of my... more This work reports on further development of an optical biosensor for the in vitro detection of mycotoxins (in particular, aflatoxin B1) using a highly sensitive planar waveguide transducer in combination with a highly specific aptamer bioreceptor. This sensor is built on a SiO 2-Si 3 N 4-SiO 2 optical planar waveguide (OPW) operating as a polarization interferometer (PI), which detects a phase shift between p-and s-components of polarized light propagating through the waveguide caused by the molecular adsorption. The refractive index sensitivity (RIS) of the recently upgraded PI experimental setup has been improved and reached values of around 9600 rad per refractive index unity (RIU), the highest RIS values reported, which enables the detection of low molecular weight analytes such as mycotoxins in very low concentrations. The biosensing tests yielded remarkable results for the detection of aflatoxin B1 in a wide range of concentrations from 1 pg/mL to 1 μg/mL in direct assay with specific DNA-based aptamers.

Journal of Electroanalytical Chemistry, 2018

One step growth of electro-assisted BSA functionalized screen-printed carbon interface with impro... more One step growth of electro-assisted BSA functionalized screen-printed carbon interface with improved antifouling characteristics. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Jeac(2017),

The International Conference on Chemical and Environmental Engineering, 2018

Toxic substances such as heavy metals and environmental pollutants may contaminate the aquatic re... more Toxic substances such as heavy metals and environmental pollutants may contaminate the aquatic resources and portable water systems, posing serious threats to the humans, agriculture, livestock and wildlife. In the last decades, discharge of industrial effluents containing xenobiotic compounds such as pharmaceuticals, endocrine disruptors, surfactants, and industrial additives to water resources has become a point of major concern for the society. The worldwide different regulatory authorities has set the regulatory standards for these contaminants with very low permissible limits i.e. pg/L or ng/kg. In consideration of toxicity and ubiquity of these compounds, the development of fast, sensitive and reliable detection methods is highly needed. Conventional analytical methods are commonly used to insure the aquatic environment safety. However, analytical figures of merit such as relatively higher limited of detection, lack of portability and unsuitability for on-site analysis, elevated cost and requirement of highly trained professionals limit their real time applications [1, 2], and are therefore difficult to implement specially in the developing countries. To overcome the above limitation, researchers have focused to design novel screening methods to maintain water quality. The current tendency has driven the development of biosensors as new analytical tools with potential to provide low cost, fast, reliable and sensitive measurements, while many of them are aimed for on-site analysis. Biosensors are defined as analytical devices incorporating a biological material, or biomimic, intimately associated with or integrated within a physicochemical transducer or transducing microsystem. The main advantages of biosensors include short analysis time, low cost per assay, possible portability, suitability for real-time/ on-site measurements. Biosensors may not completely replace the official analytical methods, but can be used both by regulatory authorities and industry to add up the information for routine testing and screening of samples. These new technologies have been applied in quantitative analysis of various target analytes for diverse applications. The present state of art suggests the evidence of several screening and biosensing methods for the detection of xeno-compounds. In this context, the current activities and results of our research group will be discussed with particular focus on the advantages and drawbacks of each developed methodologies. It is anticipated that the future will see implementation of bio sensing methods such as those based on the use of multiple array biosensors to monitor the emerging environmental contaminants.

Molecules, 2019

In the present work, an aptasensing platform was developed for the detection of a carcinogenic my... more In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electroche...

Sensors and Actuators B: Chemical, 2018

The current work is a continuation of our research targeted the development of novel optical sens... more The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant K D = 1.8. 10-8 Mol which is characteristic for highly specific aptamer/OTA binding.

Analytica chimica acta, Jan 5, 2018

The present study represents fabrication of nonstructural antibody (NS1) based immunosensor coupl... more The present study represents fabrication of nonstructural antibody (NS1) based immunosensor coupled with bovine serum albumin (BSA) modified screen printed carbon electrodes (SPCE) as transducing substrate for the early diagnosis of dengue virus. The anti-NS1 monoclonal antibody was immobilized on electro grafted BSA surface of working electrode. The electrons transfer resistance before and after NS1 attachment was monitored as a function of its concentration to perform the qualitative and quantitative analysis. The as prepared impedimetric immunosensor successfully detected the dengue virus protein with enhanced limit of detection (0.3 ng/mL) and linear range (1-200 ng/mL). The selectivity of the designed device was further elaborated with several interfering analytes and was finally demonstrated with human serum samples. The extravagant selectivity, sensitivity and easier fabrication protocol corroborate the potential applications of such immunosensor for practical diagnosis of de...

Toxins, 2018

This work focuses on the development of the novel label-free optical apta-sensors for detection o... more This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10−8–10−7 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers’ DNA chain length on their binding cha...

Toxins, Jan 11, 2018

Small molecule toxins such as mycotoxins with low molecular weight are the most widely studied bi... more Small molecule toxins such as mycotoxins with low molecular weight are the most widely studied biological toxins. These biological toxins are responsible for food poisoning and have the potential to be used as biological warfare agents at the toxic dose. Due to the poisonous nature of mycotoxins, effective analysis techniques for quantifying their toxicity are indispensable. In this context, biosensors have been emerged as a powerful tool to monitors toxins at extremely low level. Recently, biosensors based on fluorescence detection have attained special interest with the incorporation of nanomaterials. This review paper will focus on the development of fluorescence-based biosensors for mycotoxin detection, with particular emphasis on their design as well as properties such as sensitivity and specificity. A number of these fluorescent biosensors have shown promising results in food samples for the detection of mycotoxins, suggesting their future potential for food applications.

Sensors (Basel, Switzerland), Jan 24, 2017

Anti-microbial drugs are widely employed for the treatment and cure of diseases in animals, promo... more Anti-microbial drugs are widely employed for the treatment and cure of diseases in animals, promotion of animal growth, and feed efficiency. However, the scientific literature has indicated the possible presence of antimicrobial drug residues in animal-derived food, making it one of the key public concerns for food safety. Therefore, it is highly desirable to design fast and accurate methodologies to monitor antimicrobial drug residues in animal-derived food. Legislation is in place in many countries to ensure antimicrobial drug residue quantities are less than the maximum residue limits (MRL) defined on the basis of food safety. In this context, the recent years have witnessed a special interest in the field of electrochemical biosensors for food safety, based on their unique analytical features. This review article is focused on the recent progress in the domain of electrochemical biosensors to monitor antimicrobial drug residues in animal-derived food.

Sensors (Basel, Switzerland), Jan 18, 2016

Various methodologies have been reported in the literature for the qualitative and quantitative m... more Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs i...

Talanta, 2017

Lysozyme (Lys) commonly presents in wines and are known to cause toxicological impact on human he... more Lysozyme (Lys) commonly presents in wines and are known to cause toxicological impact on human health. The need of highly sensitive and reliable detection methods are evident in such matrix. In this work, we developed a competitive aptamer based assay for detection of Lys by employing carboxylated magnetic beads as a support to immobilize the target molecule Lys. The used aptamer sequence was biotinylated which further binds with Streptavidin-Alkaline phosphatase (Stp-ALP) in the micro wells. Colorimetric tests were performed in order to optimize different experimental parameters. The Lys assay showed a good linearity in the range of 5-140 nM with a limit of detection (LOD) 10 nM. The mid-point value (IC 50) 110 nM and the analysis time (60 min) validated the developed aptasensor as a promising tool for routine use. The assay displayed good recoveries of Lys in the range 99.00-99.27% and was demonstrated for the detection of Lys in wine samples. Highlights x Highly sensitive lysozyme detection route. x First report on MB coupled aptamer assay. x Assay based on unique combination of biotinylated aptamer and Streptavidin interaction.

SIMI 2016, 2016

With the increasing incidences of hazards to human health, agriculture and ecosystems, the regula... more With the increasing incidences of hazards to human health, agriculture and ecosystems, the regular monitoring of environmental contaminants such as toxins, pathogens, pharmaceuticals, resulting in various environmental borne diseases are highly recommended. As an example, according to World Health Organization (WHO), the increased mortality of food borne and waterborne diarrheal diseases resulting in 2.2 million deaths per annum, out of which mostly (1.9 million) are children's. In the recent years, the stubbornly increasing public health problems and food contamination of environmental pollutants has increased the demands of designing and fabricating the more reliable and field suitable technologies for cost effective and on-site analysis. Recently, the advances in the nucleic acid research led to the identification of specific oligonucleotides acid sequences, which are highly specific to their cognate targets and known as "aptamers". Aptamers as synthetic bio-recognition element can be in-vivo generated against a variety of analytes such as organic ions/protein/macromolecules with dissociation constants typically varying from the micro to pico-molar range. Recently, the aptamer have been widely exploited to develop the aptamerbased biosensors, commonly known as aptasensors. Aptasensors represents the novelty in the environmental and analytical science with their promising performance of high selectivity, sensitivity and stability over conventional analytical tools. In the present work, the aptasensors presented has focused on the recent advances in the development of aptamer based electrochemical or optical sensors for environmental applications with special emphasis on emerging pollutants described in the literature or develop in our laboratory BAE.

Sensors (Basel, Switzerland), Jan 6, 2016

Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aq... more Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett- Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed methodology makes use of a minimum amount of CNTs and toxic solvents, and is successfully demonstrated to form thin films over macroscopic areas of screen printed carbon tra...