nazia chaudhary - Academia.edu (original) (raw)

Papers by nazia chaudhary

Research paper thumbnail of Internal Unbonded Post-Tensioning in Direct Comparison to Internal Bonded Post-Tensioning by the Example of the Schallermuehle Viaduct

Research paper thumbnail of Plakophilin3 loss leads to an increase in autophagy and radio-resistance

Biochemical and Biophysical Research Communications

Research paper thumbnail of Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer

International Journal of Cancer, 2021

Lipocalin 2 is a siderophore‐binding protein that regulates iron homeostasis. Lipocalin 2 express... more Lipocalin 2 is a siderophore‐binding protein that regulates iron homeostasis. Lipocalin 2 expression is elevated in multiple tumor types; however, the mechanisms that drive tumor progression upon Lipocalin 2 expression remain unclear. When Lipocalin 2 is over‐expressed, it leads to resistance to 5‐fluorouracil in colon cancer cell lines in vitro and in vivo by inhibiting ferroptosis. Lipocalin 2 inhibits ferroptosis by decreasing intracellular iron levels and stimulating the expression of glutathione peroxidase4 and a component of the cysteine glutamate antiporter, xCT. The increase in xCT levels is dependent on increased levels of ETS1 in Lipocalin 2 over‐expressing cells. Inhibiting Lipocalin 2 function with a monoclonal antibody leads to a decrease in chemo‐resistance and transformation in vitro, and a decrease in tumor progression and chemo‐resistance in xenograft mouse models. Lipocalin 2 and xCT levels exhibit a positive correlation in human tumor samples suggesting that the p...

Research paper thumbnail of Tumor-specific overexpression of histone gene, H3C14 in gastric cancer is mediated through EGFR-FOXC1 axis

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

Research paper thumbnail of Thermophilic Actinomycetes from Hot Water Spring Capable of Producing Enzymes of Industrial Importance

International Journal of Research Studies in Biosciences, 2016

With the increasing demand for industrial enzymes with novel properties, the production of enzyme... more With the increasing demand for industrial enzymes with novel properties, the production of enzymes tolerant to extreme conditions has also increased. Due to their unique macromolecular properties, thermophiles possess high metabolic rates, physically and chemically stable enzymes and higher end product yields than similar mesophilic species. Actinomycetes are bacteria which require minimal nutritional requirement for their growth and hence are preferred over thermophilic bacteria as source of industrial enzymes. In this study, thermophilic actinomycetes were isolated from hot water springs situated at Vajreshwari near Mumbai, Maharashtra and screened for the production of cellulase and amylase. Five isolates of thermophilic actinomyces obtained, tested positive for both amylolytic and cellulolytic activity. Two out of the five isolates were chosen for large scale production of the enzyme. The enzyme activity of the crude extract was determined using DNSA assay for reducing sugar. The optimum conditions for activity of amylase from isolate no. 4 were 55 o C at pH 4.8 and for cellulase were 55 o C at pH 9. Amylase and cellulase with high stability and good activity at elevated temperature and acidic /alkaline conditions may find commercial applications in various fields such as such starch processing, food industries and detergents industries.

Research paper thumbnail of LCN2 and colon cancer — Have we hit the jackpot

Journal of Clinical Oncology

e15608 Background: Lipocalin2 (LCN2, also known as neutrophil gelatinase-associated lipocalin) is... more e15608 Background: Lipocalin2 (LCN2, also known as neutrophil gelatinase-associated lipocalin) is a protein that in humans is encoded by the LCN2 gene. Its abnormal expression serves critical roles in EMT transition, angiogenesis, cell migration and invasion in many cancers. We aim to assess the in vitro and in vivo effects of LCN2 as a potential chemo and radiosensitizer. Methods: Normalized RNAseq RSEM values of LCN2 were compared between normal and tumour samples from TCGA. Differences between median expression levels were assessed using Wilcoxon rank sum test. Kaplan-Meier model was used for survival analysis. Immune cell population in publicly available Colon Adenocarcinoma dataset was estimated using MCP Counter tool. Cell systems used to experimentally study the role of LCN2 in therapy resistance and tumor progression were HCT116, HT29 and DLD1. PKP3 and/or LCN2 were knocked down by shRNA. Tumor regression and therapy (5FU and radiation) sensitivity upon Anti-LCN2 treatment w...

Research paper thumbnail of Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation

Experimental cell research, Jan 24, 2018

An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To ide... more An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and this is consistent with expression data from human tumour samples suggesting that PKP3 loss correlates with an increase in LCN2 expression. PKP3 loss leads to an increase in invasion, tumour formation and metastasis and these phenotypes were dependent on the increase in LCN2 expression. The increased LCN2 expression was due to an increase in the activation of p38 MAPK in the HCT116 derived PKP3 knockdown clones as LCN2 expression decreased upon inhibition of p38 MAPK. The phosphorylated active form of p38 MAPK is translocated to the nucleus upon PKP3 loss and is dependent on complex formation between p38 MAPK and PKP3. WT PKP3 inhibits LCN2 reporter activity in PKP3 kn...

Research paper thumbnail of Internal Unbonded Post-Tensioning in Direct Comparison to Internal Bonded Post-Tensioning by the Example of the Schallermuehle Viaduct

Research paper thumbnail of Plakophilin3 loss leads to an increase in autophagy and radio-resistance

Biochemical and Biophysical Research Communications

Research paper thumbnail of Lipocalin 2 expression promotes tumor progression and therapy resistance by inhibiting ferroptosis in colorectal cancer

International Journal of Cancer, 2021

Lipocalin 2 is a siderophore‐binding protein that regulates iron homeostasis. Lipocalin 2 express... more Lipocalin 2 is a siderophore‐binding protein that regulates iron homeostasis. Lipocalin 2 expression is elevated in multiple tumor types; however, the mechanisms that drive tumor progression upon Lipocalin 2 expression remain unclear. When Lipocalin 2 is over‐expressed, it leads to resistance to 5‐fluorouracil in colon cancer cell lines in vitro and in vivo by inhibiting ferroptosis. Lipocalin 2 inhibits ferroptosis by decreasing intracellular iron levels and stimulating the expression of glutathione peroxidase4 and a component of the cysteine glutamate antiporter, xCT. The increase in xCT levels is dependent on increased levels of ETS1 in Lipocalin 2 over‐expressing cells. Inhibiting Lipocalin 2 function with a monoclonal antibody leads to a decrease in chemo‐resistance and transformation in vitro, and a decrease in tumor progression and chemo‐resistance in xenograft mouse models. Lipocalin 2 and xCT levels exhibit a positive correlation in human tumor samples suggesting that the p...

Research paper thumbnail of Tumor-specific overexpression of histone gene, H3C14 in gastric cancer is mediated through EGFR-FOXC1 axis

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

Research paper thumbnail of Thermophilic Actinomycetes from Hot Water Spring Capable of Producing Enzymes of Industrial Importance

International Journal of Research Studies in Biosciences, 2016

With the increasing demand for industrial enzymes with novel properties, the production of enzyme... more With the increasing demand for industrial enzymes with novel properties, the production of enzymes tolerant to extreme conditions has also increased. Due to their unique macromolecular properties, thermophiles possess high metabolic rates, physically and chemically stable enzymes and higher end product yields than similar mesophilic species. Actinomycetes are bacteria which require minimal nutritional requirement for their growth and hence are preferred over thermophilic bacteria as source of industrial enzymes. In this study, thermophilic actinomycetes were isolated from hot water springs situated at Vajreshwari near Mumbai, Maharashtra and screened for the production of cellulase and amylase. Five isolates of thermophilic actinomyces obtained, tested positive for both amylolytic and cellulolytic activity. Two out of the five isolates were chosen for large scale production of the enzyme. The enzyme activity of the crude extract was determined using DNSA assay for reducing sugar. The optimum conditions for activity of amylase from isolate no. 4 were 55 o C at pH 4.8 and for cellulase were 55 o C at pH 9. Amylase and cellulase with high stability and good activity at elevated temperature and acidic /alkaline conditions may find commercial applications in various fields such as such starch processing, food industries and detergents industries.

Research paper thumbnail of LCN2 and colon cancer — Have we hit the jackpot

Journal of Clinical Oncology

e15608 Background: Lipocalin2 (LCN2, also known as neutrophil gelatinase-associated lipocalin) is... more e15608 Background: Lipocalin2 (LCN2, also known as neutrophil gelatinase-associated lipocalin) is a protein that in humans is encoded by the LCN2 gene. Its abnormal expression serves critical roles in EMT transition, angiogenesis, cell migration and invasion in many cancers. We aim to assess the in vitro and in vivo effects of LCN2 as a potential chemo and radiosensitizer. Methods: Normalized RNAseq RSEM values of LCN2 were compared between normal and tumour samples from TCGA. Differences between median expression levels were assessed using Wilcoxon rank sum test. Kaplan-Meier model was used for survival analysis. Immune cell population in publicly available Colon Adenocarcinoma dataset was estimated using MCP Counter tool. Cell systems used to experimentally study the role of LCN2 in therapy resistance and tumor progression were HCT116, HT29 and DLD1. PKP3 and/or LCN2 were knocked down by shRNA. Tumor regression and therapy (5FU and radiation) sensitivity upon Anti-LCN2 treatment w...

Research paper thumbnail of Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation

Experimental cell research, Jan 24, 2018

An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To ide... more An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and this is consistent with expression data from human tumour samples suggesting that PKP3 loss correlates with an increase in LCN2 expression. PKP3 loss leads to an increase in invasion, tumour formation and metastasis and these phenotypes were dependent on the increase in LCN2 expression. The increased LCN2 expression was due to an increase in the activation of p38 MAPK in the HCT116 derived PKP3 knockdown clones as LCN2 expression decreased upon inhibition of p38 MAPK. The phosphorylated active form of p38 MAPK is translocated to the nucleus upon PKP3 loss and is dependent on complex formation between p38 MAPK and PKP3. WT PKP3 inhibits LCN2 reporter activity in PKP3 kn...