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Papers by cigdem topkaya

Journal of Clinical Case Reports, 2017

Background: Endothelial dysfunction in the maternal circulation is an early finding during preecl... more Background: Endothelial dysfunction in the maternal circulation is an early finding during preeclampsia. Early diagnosis is very important to protect from maternal and neonatal morbidity we aimed to investigate the Vascular Endothelial Growth Factor (VEGF), anti-angiogenic VEGFR-1 (sVEGFR-1) or soluble FMS-like tyrosine kinase-1 (sFlt-1), and angiogenic PlGF using uterine artery Doppler ultrasonography and to evaluate placental vascular and fetomaternal blood flow changes. Methods: This cross-sectional study included a total of 64 pregnant women aged between 15 and 40 years who were in their 20 to 24 weeks of gestation and who were at a risk of developing preeclampsia. The patients were reexamined after delivery and divided into two groups according to those who developed preeclampsia/IUGR (n=9/7) and those who did not (n=48). We compared the VEGF, sFlt1 and PlGF using uterine artery Doppler ultrasonography changes. Results: Univariate analysis results of potential factors for preeclampsia were insignificant except age (p=0.047) and body mass index (0.004). In our ROC curve, the pvalue was found to be significant. Conclusion: Our study results demonstrated that biochemical and radiologic markers did not give any clues for early diagnosis at the 20 to 24 weeks of gestation.

Clinical Chemistry, 1998

In the present study, we assessed oxidative stress in patients with dilated cardiomyopathy of isc... more In the present study, we assessed oxidative stress in patients with dilated cardiomyopathy of ischemic or idiopathic etiology. For this reason we measured whole blood reduced glutathione, erythrocyte superoxide dismutase, susceptibility of erythrocyte membranes and erythrocytes to peroxidation, and SH content of erythrocyte membranes in 12 patients (8 men and 4 women, ages 31 to 66 years) with idiopathic dilated cardiomyopathy, in 11 patients (8 men and 3 women, ages 32 to 65 years) with ischemic dilated cardiomyopathy, and in 21 healthy volunteers (12 men and 9 women, ages 25 to 67 years). There was no statistically significant difference between the two patient groups for the indicators studied (P >0.05). Blood glutathione, erythrocyte superoxide dismutase, and membrane SH content of both groups of patients was decreased compared with controls (P <0.05), whereas erythrocyte and membrane susceptibility to peroxidation were increased (P <0.05). We conclude that patients wit...

Investigative and Clinical Urology, 2019

Purpose: To investigate whether measurement of urinary calprotectin can serve as a biomarker in t... more Purpose: To investigate whether measurement of urinary calprotectin can serve as a biomarker in the diagnosis of primary bladder cancer and to confirm its diagnostic role in determining high grade and stage disease. Materials and Methods: Urinary calprotectin was measured in spot urine samples from patients with primary bladder cancer and control subjects. To confirm levels in urine, tissue samples were also obtained from bladder tumor and healthy trigone of bladder by transurethral resection in both groups. Finally, calprotectin levels in tissue and urine of the patients and control subjects were compared and their diagnostic potential was investigated in high grade and stage bladder cancers. Results: Of 82 participants, 52 were patients with bladder cancer and 30 were control subjects. The two groups were comparable in terms of age, smoking status, and comorbidities. Tissue and urinary calprotectin levels were significantly higher in the bladder cancer group. In subgroup analyses, urinary calprotectin levels were significantly higher in patients with high-grade, muscle-invasive tumors. After receiver operating characteristic analyses, the sensitivity and specificity of urinary calprotectin was 100% and 96.7%, respectively, in the diagnosis of primary bladder cancer. High grade and stage bladder cancers were detected with sensitivity and specificity of 70% and 74.2%, and 80% and 84.8%, respectively. Conclusions: Urinary calprotectin may be a valuable parameter in the diagnosis of primary bladder cancer with high sensitivity and specificity. Furthermore, it may be useful in the prediction of high grade and stage disease. However, more investigations are needed.

Clinical Laboratory, 2014

The aim of this study was to compare the use of the ideal weight with the use of the patient&... more The aim of this study was to compare the use of the ideal weight with the use of the patient&amp;amp;amp;amp;amp;amp;amp;#39;s actual weight in the C-G (Cockcroft-Gault) formula for the measurement of the GFR (Glomerular Filtration Rate). We also aimed to compare the results of the calculations explained above with the results of the MDRD (Modification of Diet in Renal Disease) formula and CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) results of the classical 24-hour creatinine clearance method. Creatinine clearance values, which were obtained from 24-hour urine collection, were compared with the values from the C-G formula in which each patient&amp;amp;amp;amp;amp;amp;amp;#39;s ideal weight was used, with the values from the C-G for- mula in which each patient&amp;amp;amp;amp;amp;amp;amp;#39;s actual body weight was used, and with the MDRD Formula and CKD-EPI. The correlation analysis between 24-hour creatinine clearance and the GFR obtained from the C-G formula with adjusted ideal weight in the Control group, Group I (patients with diabetes mellitus) and Group II resulted in values of r = 0.526, 0.576, and 0.850 (p &amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), respectively. The correlation analysis between 24-hour creatinine clearance and the MDRD formula among the same groups resulted in r = 0.814, 0.682, and 0.861 (p &amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), respectively. The correlation analysis between creatinine clearance and the CKD-EPI formula among the same groups resulted in r = 0.821, 0.679, and 0.871 (p &amp;amp;amp;amp;amp;amp;amp;lt; 0.0001), respectively. The results of the CKD-EPI formula were the most compatible with the results of 24-hour urine cre- atinine clearance which is used in clinical practice, especially in the control and diabetic group.

Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 2011

The aim of this study was to investigate the role of carbohydrate-rich drink (CHO) on perioperati... more The aim of this study was to investigate the role of carbohydrate-rich drink (CHO) on perioperative discomfort, hemodynamic changes, and insulin response in patients undergoing surgery with spinal anesthesia. Forty-four adult patients were assigned to one of the two groups of 22, namely preparation with CHO (CHO group) or fasting from midnight (control group). Ten different discomfort variables, blood glucose and insulin concentrations, and hemodynamic changes were recorded during the perioperative period. Preparation with CHO was effective in reducing hunger, thirst, malaise, unfitness, and, to some extent, anxiety (p < 0.05). Plasma glucose and insulin concentrations were increased in the CHO group (p < 0.05). Plasma glucose increased and insulin decreased in the control group (p < 0.05). In the control group, mean arterial pressure was lower compared to the CHO group (p < 0.05). Preparation with CHO before spinal anesthesia is advantageous due to reducing perioperativ...

Turkiye Klinikleri Journal of Medical Sciences, 2012

In this study, we investigated a cartilage degradation marker; cross-linked C-terminal telopeptid... more In this study, we investigated a cartilage degradation marker; cross-linked C-terminal telopeptide of collagen, CTX-I (serum) and CTX-II (urine) along with other laboratory variables in postmenopausal women with osteoarthritis (OA) of the knee with/without associated osteoporosis (OP). In addition, we aimed to investigate whether CTX-II which is released into the synovial fluid and systemic circulation and excreted by urine was an effective marker for the diagnosis of OA. Furthermore, we evaluated a possible relation of CTX-II with radiological grades of OA. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Postmenopausal women without any history of OA or OP costituted the control group (n=21). Study Group I consisted of 30 post-menopausal women with primary OA of the knee, and Group II consisted of 14 postmenopausal women with primary OA of the knee along with OP. Group I and Group II were categorized by radiological stages according to Kellgren-Lawrence system as stage 2, stage 3 and stage 4. R Re es su ul lt ts s: : The control group was not statistically significantly different from Group I, II, or radiologically 2 nd , 3 rd , 4 th stage of OA groups for age, body mass index, duration of menopause or levels of alkaline phosphatase, erythrocyte sedimentation rate, C-reactive protein, osteocalcin, CTX-I and CTX-II. The results of CTX-II values (ng/mmol) of the control and staged groups were as follows; median (25th-75th) level:341 (216-850); 609.34 (363-1072); 492 (319-927); 928 (400-1245) respectively; (p=0.210). Percentage change of median values between the control group and group I, II and radiologically 2 nd , 3 th , 4 th stage of OA groups were 61%+, 80%+, 78%+, 44%,+ 172%+, respectively. C Co on nc cl lu us si io on n: : CTX-II levels of the control group were similar to those of the knee osteoarthritis patients with or without osteoporosis as well as radiologically graded subgroups, and statistical analysis did not show statistically significant differences. , However, percentage change of median values between the control group and group I, II, and between the control group and radiologically 2 nd , 3 th , 4 th stage of OA groups were significant. K Ke ey y W Wo or rd ds s: : Postmenopause; osteoporosis; osteoarthritis, knee; collagen type I trimeric cross-linked peptide Ö ÖZ ZE ET T A Am ma aç ç: : Bu çalışmada osteoporozun (OP) eşlik ettiği veya etmediği diz osteoartritli (OA) postmenopozal kadınlarda diğer laboratuvar değişkenlerinin yanı sıra kollagen çapraz bağlı C-terminal telopeptidi CTX-I (serum) ve CTX-II (idrar) olarak adlandırılan kıkırdak parçalanma belirtecini araştırdık. Sinovyal sıvı ve sistemik dolaşıma salınan ve idrarla atılan CTX-II'nin OA tanısı için etkili bir belirteç olup olmadığını araştırmayı da amaçladık. Ayrıca CTX-II ile OA'in radyolojik dereceleri arasında olası bir ilişkiyi değerlendirdik. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : OA veya OP öyküsü olmayan postmenopozal kadınlar kontrol grubunu oluşturdu (n=21). Çalışma grubu I primer diz OA olan 30 postmenopozal kadından oluşurken, Grup II OP'nin yanı sıra primer diz OA'sı olan 14 kadından oluşuyordu. Grup I ve Grup II Kellgren-Lawrence sistemine göre evre 2, evre 3 ve evre 4 olarak radyolojik olarak kategorize edildi. B Bu ul lg gu ul la ar r: : Kontrol grubu ve Grup I, II ve radyolojik olarak 2., 3., 4. evre OA grupları arasında özellikler ve yaş, beden kitle indeksi, menopoz süresi ve alkalen fosfataz, eritrosit sedimentasyon hızı, C-reaktif protein, osteokalsin, CTX-I ve CTX-II düzeyleri gibi hasta verileri bakımından istatistiksel olarak anlamlı fark bulmadık. Kontrol ve evre gruplarının CTX-II değerleri (ng/mmol) sonuçları aşağıdaki gibiydi: Ortanca olarak sırasıyla (25-75): 341 (216-850); 609,34 (363-1072); 492 (319-927); 928 (400-1245); (p=0,210). Kontrol grubu ve grup I, II ve radyolojik olarak 2., 3., 4. evre OA grupları arasında ortanca değerlerin yüzde değişimi sırasıyla %61+, %80+, %78+, %44+, %172+ idi. S So on nu uç ç: : Osteoporozu olan ve olmayan diz osteoartriti hastaların ve radyolojik olarak derecelendirilmiş alt grupların CTX-II düzeyleri kontrol grubuna göre istatistiksel olarak anlamlı fark göstermedi. Fakat kontrol grubu ve grup I,II arasında ve kontrol grubu ve radyolojik olarak 2., 3., 4. evre OA grupları arasında ortanca değerlerin yüzde değişimi belirgindi. A An na ah ht ta ar r K Ke el li im me el le er r: : Postmenopoz; osteoporoz; osteoartrit,diz; kollajen tip I üç parçalı çaprazlayan peptid T Tu ur rk ki iy ye e K Kl li in ni ik kl le er ri i J J M Me ed d S Sc ci i 2 20 01 12 2; ;3 32 2((2 2)): :4 45 59 9-6 65 5

Istanbul Medical Journal, 2011

Klinik biyokimya analiz sonuçlarını etkileyen birçok faktör tanımlanmıştır. Lipemi de biyokimya s... more Klinik biyokimya analiz sonuçlarını etkileyen birçok faktör tanımlanmıştır. Lipemi de biyokimya sonuçlarını etkileyen bu faktörler arasında önemli bir yer tutmaktadır. Bu çalışmada, laboratuvarımızda kullandığımız Simens Advia sistemlerindeki glukoz ölçümlerinde eksojen lipit ilavesiyle bildirilen lipemi interferansını araştırmayı planladık. Gereç ve Yöntem: Çalışmamızda eksojen lipit ilavesi yerine, trigliserit (TG) düzeyi yüksek hastalardan oluşturduğumuz 5 farklı düzeydeki serum havuzunu kullandık ve farklı glukoz konsantrasyonları için dışardan glukoz ilave ettik. Bulgular: Normal glukoz düzeylerindeki havuzlarımızın referans yöntem (hekzokinaz yöntemi) ile karşılaştırıldığında pozitif % değişim oranları sırasıyla: 2,28; 2,08; 2,11; 2,10; 2,85 iken; glukoz ilavesiyle düzeyi 160 mg/dl'ye getirilen havuzlarda % değişim oranları: 3,17; 2,97; 3,09; 3,09; 4,06 olarak bulundu. Sonuç: Çalışma sonuçlarımız, lipemi interferansı çalışmalarında eksojen ve endojen TG kullanımının farklı interferans oranları bildirimine yol açabileceği görüşünü desteklemektedir.

Journal of Pediatric Endocrinology and Metabolism, 2007

Obesity is a common health problem that is rapidly increasing among children. Obesity is accompan... more Obesity is a common health problem that is rapidly increasing among children. Obesity is accompanied by a high incidence of atherosclerosis, arterial hypertension and type 2 diabetes mellitus. We tested the hypothesis that the risk for atherosclerosis may be increased in obese children and measured some risk factors for atherosclerosis. We determined sialic acid levels and investigated correlations with malondialdehyde (MDA), susceptibility to oxidation, total thiol concentrations, glucose and lipid profile in 39 obese (BMI 26.6 +/- 3.9) and 33 sex- and age-matched healthy children (BMI 15.9 +/- 1.7). MDA concentrations, susceptibility to oxidation and hs-CRP were significantly higher in obese children than controls. Sialic acid and total thiol concentrations were higher in controls but this did not reach statistical significance. Total cholesterol, LDL-cholesterol and glucose concentrations were significantly higher in the obese group and there was a positive correlation between BMI and MDA, susceptibility to oxidation, hs-CRP and glucose concentrations, and a negative correlation between BMI and HDL-cholesterol. Although sialic acid levels were not different between the groups, they showed a correlation with hs-CRP. A higher risk was found in obese children in relation to oxidative stress parameters, hs-CRP and lipid profile, and this risk showed a positive correlation with BMI. These results are important because children will encounter this increased risk for a longer time than adults, and taking care of obesity in childhood is especially important.

Journal of Neurosurgical Anesthesiology, 2008

We tested dexmedetomidine, an alpha2 agonist, for its ability to decrease heart rate, arterial bl... more We tested dexmedetomidine, an alpha2 agonist, for its ability to decrease heart rate, arterial blood pressure, and neuroendocrinal responses to skull-pin head-holder application during craniotomy. In a randomized, double-blinded, placebo-controlled study, 40 patients undergoing craniotomy with attachment of a pin head-holder were randomly assigned to one of 2 equal groups. The placebo group received saline, whereas the treatment group (DEX group) received a single bolus dose of dexmedetomidine (1 microg/kg) intravenously over 10 minutes before induction of anesthesia. Arterial blood pressure, heart rate, and sequential concentrations of circulating cortisol, prolactin, insulin, and blood glucose were measured. Relative to baseline and the other group, arterial blood pressure and heart rate decreased significantly after the administration of dexmedetomidine through skull pinning (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). In the placebo group, patients&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; heart rate and arterial blood pressure measures increased at 1 and 5 minutes after skull-pin insertion, compared with baseline and the DEX group (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). In both groups, plasma cortisol, prolactin, and blood glucose increased significantly relative to baseline after skull-pin insertion. However, the values were significantly higher in the placebo group compared with the DEX group (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). Although insulin levels were not significantly altered in the DEX group, the plasma concentrations of insulin decreased significantly after pin insertion in the placebo group. Our results suggested that, a single bolus dose of dexmedetomidine before induction of anesthesia attenuated the hemodynamic and neuroendocrinal responses to skull-pin insertion in patients undergoing craniotomy.

Annals of Clinical Biochemistry, 2009

Background: Percutaneous coronary intervention (PCI) is accepted as a model of myocardial ischaem... more Background: Percutaneous coronary intervention (PCI) is accepted as a model of myocardial ischaemia in studies of ischaemia markers, especially of ischaemia-modified albumin (IMA). However, there is concern that IMA levels may reflect changes in albumin concentrations rather than myocardial ischaemia also during PCI. Methods: Twenty-one consecutive patients (17 men and 4 women) undergoing single-vessel percutaneous coronary angioplasty were enrolled in the study. IMA and albumin levels were measured together with myoglobin, creatine kinase 2 and cardiac troponin I, before (Group 1), immediately after (Group 2) and 6 h after (Group 3) the procedure of PCI. Results: The IMA levels of Group 2 were significantly higher than those of Group 1 and Group 3 (P , 0.05 for both). However, correction of IMA by multiplying with the (individual albumin concentration of the patient/median albumin concentration of Group 1) ratio gave no statistical differences between the groups (P. 0.05). There were strong negative correlations between IMA levels and albumin concentrations within individual groups (r

Clinical Chemical Laboratory Medicine, 2008

Background: Cardiomyopathy (CMP) is a common debilitating illness, associated with a high mortali... more Background: Cardiomyopathy (CMP) is a common debilitating illness, associated with a high mortality and poor quality of life. There is extensive evidence from in vitro and animal experiments that CMP is a state of increased oxidative stress. Coenzyme Q10 (CoQ10) and high-sensitivity C-reactive protein (hs-CRP) are important markers to evaluate the oxidative stress and inflammatory status of patients with CMP. Methods: A total of 28 patients with chronic stable heart failure (21 men and 7 women, ages 18-76 years) were included in the study. Causes of heart failure were ischemic CMP in 17 patients and idiopathic dilated CMP in 11 patients. A total of 28 patients (12 men and 16 women; ages 30-71 years) with normal coronary angiography were enrolled as a control group. Levels of CoQ10, albumin, total thiol groups (T-SH), bilirubin, uric acid as plasma antioxidants, hs-CRP as an inflammation marker and lipid profile were studied in patients and controls. Results: Plasma CoQ10, T-SH and albumin levels were significantly decreased in patients compared to controls. Uric acid, bilirubin and hs-CRP levels were found to be significantly increased compared to controls. Conclusions: In this study, evidence of decreased antioxidant status was determined in CMP patients together with vascular inflammation. CoQ10, other plasma antioxidants and hs-CRP measured routinely can reflect decreased antioxidant status and inflammatory process in patients with dilated CMP. These markers can be used to monitor the status of patients with CMP.

Gazi Medical Journal, 2008

b2-glycoprotein I is a glycoprotein that is normally present in the plasma, but its physiologic s... more b2-glycoprotein I is a glycoprotein that is normally present in the plasma, but its physiologic significance is unknown. In vitro, it is shown to inhibit prothrombinase activity, contact pathway activation, ADP induced thrombocyte aggregation, and factor Xa formation by thrombocytes. Therefore, it is a weak, natural anticoagulant.11,12 In SLE, b2-glycoprotein I has autoantigenic properties for both B and T lymphocytes.13,14 In a multi-center study in Europe, medium-high titers of aCL IgG and anti-b2GP1 were found to be associated with thrombosis,

Clinical laboratory, 2014

Advanced oxidation protein product (AOPP) was first described as an oxidative protein marker in c... more Advanced oxidation protein product (AOPP) was first described as an oxidative protein marker in chronic uremic patients and measured with a semi-automatic end-point method. Subsequently, the kinetic method was introduced for AOPP assay. We aimed to compare these two methods by adapting them to a chemistry analyzer and to investigate the correlation between AOPP and fibrinogen, the key molecule responsible for human plasma AOPP reactivity, microalbumin, and HbA1c in patients with type II diabetes mellitus (DM II). The effects of EDTA and citrate-anticogulated tubes on these two methods were incorporated into the study. This study included 93 DM II patients (36 women, 57 men) with HbA1c levels > or = 7%, who were admitted to the diabetes and nephrology clinics. The samples were collected in EDTA and in citrate-anticoagulated tubes. Both methods were adapted to a chemistry analyzer and the samples were studied in parallel. In both types of samples, we found a moderate correlation be...

European Journal of Anaesthesiology, 2007

Scandinavian Journal of Clinical & Laboratory Investigation, 2002

The heart cannot supply sufficient blood for tissue metabolic needs in patients with congestive h... more The heart cannot supply sufficient blood for tissue metabolic needs in patients with congestive heart failure. Hypoxia and organ hypoperfusion increase oxidative activity. It has been reported that free radicals are involved in the genesis of heart failure. The aim of this study was to assess the status of oxidative stress by simple measurements in patients with dilated cardiomyopathy of ischemic or idiopathic etiology. Eleven patients (8 M, 3 F, age range 32 to 65 years) with dilated cardiomyopathy of ischemic etiology and 12 patients (8 M, 4 F, age range 31 to 66 years) with dilated cardiomyopathy of idiopathic etiology were included in the study. A control group included 21 healthy subjects (12 M, 9 F, age range 25 to 67 years). Levels of thiobarbituric acid-reactive substances, total thiols, and fluorescent products of lipid peroxidation were measured in plasma/serum samples of patients and controls. No statistically significant differences were found between the two patient groups for the parameters studied (p>0.05). Levels of thiobarbituric acid-reactive substances and fluorescent products of lipid peroxidation were higher in both patient groups than in controls (p<0.05), whereas concentrations of total thiols were decreased (p<0.05). In conclusion, in patients with idiopathic or ischemic dilated cardiomyopathy, there are associated abnormalities of a range of markers of increased oxidative stress and lipid peroxidation. The plasma/serum constituents studied can be routinely measured in order to monitor patients during antioxidant therapy.