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Papers by cristian flores

Research paper thumbnail of Plataforma educativa comunitaria para el desarrollo de energías renovables en la reserva de biosfera Yabotí, Misiones

ReDDI: Revista Digital del Departamento de Ingeniería

El siguiente trabajo es un proyecto de extension interdisciplinario que presenta la metodologia u... more El siguiente trabajo es un proyecto de extension interdisciplinario que presenta la metodologia utilizada y los resultados obtenidos en el desarrollo de la plataforma educativa comunitaria para el desarrollo de energias renovables efectuado en la Colonia Pepiri, dentro de la Reserva de Biosfera Yaboti, El Soberbio, Misiones, Argentina. En la actualidad y en virtud de la crisis energetica, se impulsa la creacion de prototipos demostrativos de pequena escala que permitan resolver parcialmente el deficit energetico y oficien de plataforma educativa para las comunidades. La implantacion del prototipo de microturbina hidraulica en el salto del Arroyo Trigeno para autogeneracion electrica es la primera iniciativa en esa direccion.

Research paper thumbnail of Linking Open Access Movement to the Indonesian Islamic Higher Education

Proceedings of the 3rd Asian Education Symposium (AES 2018), 2019

Research paper thumbnail of Linking Open Access Movement to the Indonesian Islamic Higher Education

Proceedings of the 3rd Asian Education Symposium (AES 2018), 2019

Research paper thumbnail of Ultrasound-guided intervention in thoracic pathology

Introduction: The utility of ultrasound scans for thoracic conditions is nowadays demonstrated. I... more Introduction: The utility of ultrasound scans for thoracic conditions is nowadays demonstrated. It has a series of advantages over other imaging techniques, which include the absence of ionising radiation, the possibility of bedside exploration without moving the patient, real-time assessment and equipment accessibility. These advantages make it the study of choice for guiding minimally-invasive procedures in several thoracic conditions, thus achieving the management of outpatients, avoiding hospitalizations or conventional surgical interventions, and therefore minimizing health system costs. Objective: Highlight the role of ultrasound scans and of minimally-invasive methods for the various thoracic conditions. Design: Retrospective, observational, descriptive study. Material and method: Bibliographic review and assessment of patients treated in Thoracic Surgery Service. Results: Between January 2011 and March 2012, 19 ultrasoundguided thoracic minimally-invasive procedures were car...

Research paper thumbnail of 36) 実験的低および高K血症と腎機能(第13回日本循環器学会関東・甲信越地方学会総会)

Japanese Circulation Journal-english Edition, 1960

Research paper thumbnail of Unidad piloto educativa y comunitaria para el desarrollo de energías renovables

El siguiente trabajo es un proyecto de extensión interdisciplinario que presenta la metodología u... more El siguiente trabajo es un proyecto de extensión interdisciplinario que presenta la metodología utilizada en el desarrollo de la unidad piloto educativa y comunitaria para el desarrollo de energías renovables efectuado en la Colonia Pepirí, dentro de la Reserva de Biosfera Yabotí, El Soberbio, Misiones, Argentina. Presentando alternativas de generación eléctrica que permitan darles luz a las personas de la zona observada. Los resultados experimentales obtenidos demuestran que el fomento de la autogeneración de energía a pequeña escala y las prácticas educativas en zonas naturales protegidas, permiten el abastecimiento energético de manera limpia y no contaminante, a fin de priorizar el ambiente y resolver de esta forma antiguas prácticas predatorias.

Research paper thumbnail of 200) 馬杉腎炎における機能的, 組織學的および電氣顯微鏡的研究(第21回日本循環器學曾總曾)

Research paper thumbnail of 230) Coil型人工腎臟の實驗と臨床的鷹用(第21回日本循環器學會總會)

Research paper thumbnail of Siringoma Condroide Maligno: a Propósito de un Caso Malignant chondroid syringoma: Case Report

Introduction: Although the malignant chondroid syringoma is a very rare skin tumor, it must be co... more Introduction: Although the malignant chondroid syringoma is a very rare skin tumor, it must be considered in the differential diagnosis of axillary tumors. Objectives: The following clinical case is analyzed and presented in order to consider this entity as differential diagnosis of axillary tumors. Case Report: 82 year-old, female patient who presents with a skin tumor of 3 years evolution in the right axilla. Biopsy (PUNCH) reports infiltration due to moderately differentiated carcinoma. Surgical extirpation is decided. Discussion: The malignant chondroid syringoma is a rare tumor, but can metastasize, with extended survival times. It prefers limbs and trunk. Its prognosis is good when the extirpation is complete, but patients must be followed-up anyway.

Research paper thumbnail of MON-LB6 Association Between Sex Steroid and Metabolic Parameters in Cord Blood With Placental Fatty Acid Transporter in Obese Pregnant Women

Journal of the Endocrine Society, 2020

Obesity reduces maternal insulin sensitivity and alters sex steroid serum concentrations. However... more Obesity reduces maternal insulin sensitivity and alters sex steroid serum concentrations. However, it is not clear if these changes are reflected in the fetal circulation. On the other hand, similar to other metabolic tissues, modifications in sex steroid concentrations and metabolic parameters could modify the transport and metabolism of fatty acids (FA) in the placenta increasing their availability for the fetus. Therefore, we aimed to study, in pregnant women with normal-weight and obesity, sex steroid serum concentrations in cord blood and their relationship with the gene expression of FA transporters and of molecules related with FA metabolism in the placenta. We included 26 pregnant women with normal-weight and 26 pregnant women with obesity without pregnancy complications. At term of pregnancy, mixed cord blood and placenta samples were collected and stored at -80°C. Serum concentrations of dehydroepiandrosterone (DHEA), DHEA sulfate (DHEAS), androstenedione, testosterone, es...

Research paper thumbnail of SUN-047 Cosmetic Injection of Silicone in a Transgender Person Leading to Granulomatous Disease with Hypercalcemia and Terminal Kidney Failure

Journal of the Endocrine Society, 2020

Background: Granulomatous disease secondary to cosmetic injection of silicone is an uncommon caus... more Background: Granulomatous disease secondary to cosmetic injection of silicone is an uncommon cause of hypercalcemia. Transgender persons with limited access to appropriate surgery commonly use this procedure as an alternative, which can cause serious complications. Clinical Case: Forty year old transgender women with a 7-year history of nephrolithiasis treated with lithotripsy, pyelotomy and requiring the installation of a pig tail. She is admitted at the emergency room due to renal failure. Non-contrast CT rules out acute obstruction and lab tests conclude terminal kidney failure so she is started with dialysis and discharged. Two months later she returns with fever, she is diagnosed with central venous catheter-related bloodstream infection associated to the dialysis catheter and is started with antibiotics. Physical exam reveals pigmented, indurated nodular areas in her buttocks, hips and legs. The patient is re-interrogated and admits to have had injections of industrial silicon...

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of Design of a piping system to measure constant accessories

The values of the constant accessories are huge importance to calculate the hydraulic losses in a... more The values of the constant accessories are huge importance to calculate the hydraulic losses in a pipeline system when a fluid is transported, i.e., the flow rates, diameters, lengths and load losses can be determined in sections considering the characteristics of fluid. Here, we analyze the water hardness to choose the most appropriated material for pipes and fittings, which allows the design and construction of an appropriated pipe system. With the system in operation, the pressure is measured before and after the passage through the different accessories, thus, the pressure losses of constants are computed by using the Bernoulli equation. To validate the values of the constants on the different accessories of the proposed system, we compare the data with the values reported in the literature (90° Elbow, check valve, ball valve, butterfly valve and T). Furthermore, a reasonable relative error percentage of 9.73 %, 12.24 %, 19.88 %, 16.48 % and 19.32 % respectively, is observed. The latter can be attributed to the points at which the data are taken could be not adequate. With this in mind, the proposed system can be improved by changing the experimental set up or measurement instruments. However, this work can be employed as a useful instructive guideline for future

Research paper thumbnail of Marshall parameters for quality control of hot mix asphalt after pavement construction

Revista de la construcción, 2019

Highways have been and are built using Hot Mix Asphalt (HMA) as surface layer. The most common HM... more Highways have been and are built using Hot Mix Asphalt (HMA) as surface layer. The most common HMA design procedure is the Marshall Method, a method also used for quality control (QC) at the time of road construction. Due to several factors, sometimes it is not possible to sample the surface layer when constructed. When this occurs, QC is performed after construction by analyzing cores. Current regulations, however, fail having clear procedures regarding the collection and analysis of pavement cores after the complete placement of the asphalt mixture. For this reason, this study proposes a comparative analysis of Marshall parameters, mixture densities, and aggregates gradation obtained from specimens obtained during construction, versus cores samples extracted 30 and 60 days later. The research revealed that all the parameters measured on core samples, except flow, could be used to approve asphalt paving works after its construction.

Research paper thumbnail of Diversity of genomic variants and population genetics of ethnic and regional groups across Russia

импозиум «Биоразнообразие: геномика и эволюция», 2018

Research paper thumbnail of Abstracts from the 13th WINFOCUS World Congress on Ultrasound in Emergency & Critical Care

Critical ultrasound journal, Jan 21, 2017

Background: Though an extremely rare complication of arterial cannulation, the incidence of radia... more Background: Though an extremely rare complication of arterial cannulation, the incidence of radial pseudoaneurysm may increase with the growing use of extended radial artery access for coronary angiography. Case report: A 57 year-old female presented to the emergency department with painful swelling to the volar radial surface of her right wrist 5 days after a non-emergent transradial coronary angiography. An emergency physician used point-of-care ultrasound to diagnose a radial artery pseudoaneurysm. The high-frequency linear transducer allowed visualization of the arterial wall defect and connection between artery and hematoma on B-mode, turbulent pulsatile flow into the adjacent hematoma using color flow Doppler, and a to-and-fro waveform at the wall defect using spectral Doppler. Due to the size and characteristics of the pseudoaneurysm, as well as her pain and mild distal sensory deficits, it was determined that the patient required prompt operative repair. Discussion: Bedside ultrasound is the most rapid and dynamic imaging modality for making diagnosing a radial artery pseudoaneurysm. Different techniques for treating this condition include conservative care, extended compression, thrombin injection, and surgery. Management primarily depends on the size of the pseudoaneurysm and its associated symptoms. Conclusion: In addition to understanding the pathophysiology and risk factors for this condition, the emergency physician must be adept at using point-of-care ultrasound to both make the diagnosis and characterize its findings to determine management. Consent for publication: The authors confirm that written informed consent was obtained for publication. A2 Type A thoracic aortic dissection suspected on resident performed bedside transthoracic echocardiography (TTE) in a patient with initial electrocardiogram (ECG) demonstrating an ST elevation myocardial infarction (STEMI)

Research paper thumbnail of Análise Comparativa Entre a Modalidade Cold-Wire e Hot- Wire No Processo De Soldagem Tig

Anais do IX Congresso Brasileiro de Engenharia de Fabricação, 2017

Research paper thumbnail of Testosterone increases CCL-2 expression in visceral adipose tissue from obese women of reproductive age

Molecular and cellular endocrinology, Mar 15, 2017

Hyperandrogenic states and obesity in women are associated with insulin-resistance. Androgens red... more Hyperandrogenic states and obesity in women are associated with insulin-resistance. Androgens reduce glucose uptake in adipose cells and increase TNFα production in peripheral monocytes. Inflammatory cytokines have a known detrimental effect on insulin resistance. The aim of the present study was to explore the role of testosterone in local cytokine production in visceral adipose tissue from women of reproductive age. Twenty-four women 18-40 years old, undergoing elective abdominal surgery for benign and non-inflammatory conditions, were recruited for the study. Women with clinical hyperandrogenism, diabetes, hepatic or renal dysfunction, hypothyroidism, BMI> 40 or drugs known to interfere with hormonal levels or fat metabolism were excluded. Women were classified into two groups according to BMI, non-obese (NO; BMI < 30) and obese (O; BMI 30-40). A basal blood sample was drawn at the time of surgery for the measurement of glucose, insulin, total testosterone, lipid profile an...

Research paper thumbnail of Plataforma educativa comunitaria para el desarrollo de energías renovables en la reserva de biosfera Yabotí, Misiones

ReDDI: Revista Digital del Departamento de Ingeniería

El siguiente trabajo es un proyecto de extension interdisciplinario que presenta la metodologia u... more El siguiente trabajo es un proyecto de extension interdisciplinario que presenta la metodologia utilizada y los resultados obtenidos en el desarrollo de la plataforma educativa comunitaria para el desarrollo de energias renovables efectuado en la Colonia Pepiri, dentro de la Reserva de Biosfera Yaboti, El Soberbio, Misiones, Argentina. En la actualidad y en virtud de la crisis energetica, se impulsa la creacion de prototipos demostrativos de pequena escala que permitan resolver parcialmente el deficit energetico y oficien de plataforma educativa para las comunidades. La implantacion del prototipo de microturbina hidraulica en el salto del Arroyo Trigeno para autogeneracion electrica es la primera iniciativa en esa direccion.

Research paper thumbnail of Linking Open Access Movement to the Indonesian Islamic Higher Education

Proceedings of the 3rd Asian Education Symposium (AES 2018), 2019

Research paper thumbnail of Linking Open Access Movement to the Indonesian Islamic Higher Education

Proceedings of the 3rd Asian Education Symposium (AES 2018), 2019

Research paper thumbnail of Ultrasound-guided intervention in thoracic pathology

Introduction: The utility of ultrasound scans for thoracic conditions is nowadays demonstrated. I... more Introduction: The utility of ultrasound scans for thoracic conditions is nowadays demonstrated. It has a series of advantages over other imaging techniques, which include the absence of ionising radiation, the possibility of bedside exploration without moving the patient, real-time assessment and equipment accessibility. These advantages make it the study of choice for guiding minimally-invasive procedures in several thoracic conditions, thus achieving the management of outpatients, avoiding hospitalizations or conventional surgical interventions, and therefore minimizing health system costs. Objective: Highlight the role of ultrasound scans and of minimally-invasive methods for the various thoracic conditions. Design: Retrospective, observational, descriptive study. Material and method: Bibliographic review and assessment of patients treated in Thoracic Surgery Service. Results: Between January 2011 and March 2012, 19 ultrasoundguided thoracic minimally-invasive procedures were car...

Research paper thumbnail of 36) 実験的低および高K血症と腎機能(第13回日本循環器学会関東・甲信越地方学会総会)

Japanese Circulation Journal-english Edition, 1960

Research paper thumbnail of Unidad piloto educativa y comunitaria para el desarrollo de energías renovables

El siguiente trabajo es un proyecto de extensión interdisciplinario que presenta la metodología u... more El siguiente trabajo es un proyecto de extensión interdisciplinario que presenta la metodología utilizada en el desarrollo de la unidad piloto educativa y comunitaria para el desarrollo de energías renovables efectuado en la Colonia Pepirí, dentro de la Reserva de Biosfera Yabotí, El Soberbio, Misiones, Argentina. Presentando alternativas de generación eléctrica que permitan darles luz a las personas de la zona observada. Los resultados experimentales obtenidos demuestran que el fomento de la autogeneración de energía a pequeña escala y las prácticas educativas en zonas naturales protegidas, permiten el abastecimiento energético de manera limpia y no contaminante, a fin de priorizar el ambiente y resolver de esta forma antiguas prácticas predatorias.

Research paper thumbnail of 200) 馬杉腎炎における機能的, 組織學的および電氣顯微鏡的研究(第21回日本循環器學曾總曾)

Research paper thumbnail of 230) Coil型人工腎臟の實驗と臨床的鷹用(第21回日本循環器學會總會)

Research paper thumbnail of Siringoma Condroide Maligno: a Propósito de un Caso Malignant chondroid syringoma: Case Report

Introduction: Although the malignant chondroid syringoma is a very rare skin tumor, it must be co... more Introduction: Although the malignant chondroid syringoma is a very rare skin tumor, it must be considered in the differential diagnosis of axillary tumors. Objectives: The following clinical case is analyzed and presented in order to consider this entity as differential diagnosis of axillary tumors. Case Report: 82 year-old, female patient who presents with a skin tumor of 3 years evolution in the right axilla. Biopsy (PUNCH) reports infiltration due to moderately differentiated carcinoma. Surgical extirpation is decided. Discussion: The malignant chondroid syringoma is a rare tumor, but can metastasize, with extended survival times. It prefers limbs and trunk. Its prognosis is good when the extirpation is complete, but patients must be followed-up anyway.

Research paper thumbnail of MON-LB6 Association Between Sex Steroid and Metabolic Parameters in Cord Blood With Placental Fatty Acid Transporter in Obese Pregnant Women

Journal of the Endocrine Society, 2020

Obesity reduces maternal insulin sensitivity and alters sex steroid serum concentrations. However... more Obesity reduces maternal insulin sensitivity and alters sex steroid serum concentrations. However, it is not clear if these changes are reflected in the fetal circulation. On the other hand, similar to other metabolic tissues, modifications in sex steroid concentrations and metabolic parameters could modify the transport and metabolism of fatty acids (FA) in the placenta increasing their availability for the fetus. Therefore, we aimed to study, in pregnant women with normal-weight and obesity, sex steroid serum concentrations in cord blood and their relationship with the gene expression of FA transporters and of molecules related with FA metabolism in the placenta. We included 26 pregnant women with normal-weight and 26 pregnant women with obesity without pregnancy complications. At term of pregnancy, mixed cord blood and placenta samples were collected and stored at -80°C. Serum concentrations of dehydroepiandrosterone (DHEA), DHEA sulfate (DHEAS), androstenedione, testosterone, es...

Research paper thumbnail of SUN-047 Cosmetic Injection of Silicone in a Transgender Person Leading to Granulomatous Disease with Hypercalcemia and Terminal Kidney Failure

Journal of the Endocrine Society, 2020

Background: Granulomatous disease secondary to cosmetic injection of silicone is an uncommon caus... more Background: Granulomatous disease secondary to cosmetic injection of silicone is an uncommon cause of hypercalcemia. Transgender persons with limited access to appropriate surgery commonly use this procedure as an alternative, which can cause serious complications. Clinical Case: Forty year old transgender women with a 7-year history of nephrolithiasis treated with lithotripsy, pyelotomy and requiring the installation of a pig tail. She is admitted at the emergency room due to renal failure. Non-contrast CT rules out acute obstruction and lab tests conclude terminal kidney failure so she is started with dialysis and discharged. Two months later she returns with fever, she is diagnosed with central venous catheter-related bloodstream infection associated to the dialysis catheter and is started with antibiotics. Physical exam reveals pigmented, indurated nodular areas in her buttocks, hips and legs. The patient is re-interrogated and admits to have had injections of industrial silicon...

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of DNA methylation in promoter regions of genes involved in the reproductive and metabolic function of children born to women with PCOS

Epigenetics, 2020

Clinical and experimental evidences indicate that epigenetic modifications induced by the prenata... more Clinical and experimental evidences indicate that epigenetic modifications induced by the prenatal environment are related to metabolic and reproductive derangements in polycystic ovary syndrome (PCOS). Alterations in the leptin and adiponectin systems, androgen signalling and antimüllerian hormone (AMH) levels have been observed in PCOS women and in their offspring. Using a targeted Next-Generation Sequencing (NGS), we studied DNA methylation in promoter regions of the leptin (LEP), leptin receptor (LEPR), adiponectin (ADIPOQ), adiponectin receptor 1 and 2 (ADIPOR1 and ADIPOR2), AMH and androgen receptor (AR) genes in 24 sons and daughters of women with PCOS (12 treated with metformin during pregnancy) and 24 children born to non-PCOS women during early infancy (2-3 months of age). Genomic DNA was extracted from whole blood, bisulphite converted and sequenced by NGS. Girls showed differences between groups in 1 CpG site of LEPR, 2 of LEP, 1 of ADIPOR2 and 2 of AR. Boys showed differences in 5 CpG sites of LEP, 3 of AMH and 9 of AR. Maternal metformin treatment prevented some of these changes in LEP, ADIPOR2 and partially in AR in girls, and in LEP and AMH in boys. Maternal BMI at early pregnancy was inversely correlated with the methylation levels of the ChrX-67544981 site in the whole group of girls (r = −0.530, p = 0.008) and with the global Z-score in all boys (r = −0.539, p = 0.007). These data indicate that the intrauterine PCOS environment predisposes the offspring to acquire certain sex-dependent DNA methylation patterns in the promoter regions of metabolic and reproductive genes.

Research paper thumbnail of Design of a piping system to measure constant accessories

The values of the constant accessories are huge importance to calculate the hydraulic losses in a... more The values of the constant accessories are huge importance to calculate the hydraulic losses in a pipeline system when a fluid is transported, i.e., the flow rates, diameters, lengths and load losses can be determined in sections considering the characteristics of fluid. Here, we analyze the water hardness to choose the most appropriated material for pipes and fittings, which allows the design and construction of an appropriated pipe system. With the system in operation, the pressure is measured before and after the passage through the different accessories, thus, the pressure losses of constants are computed by using the Bernoulli equation. To validate the values of the constants on the different accessories of the proposed system, we compare the data with the values reported in the literature (90° Elbow, check valve, ball valve, butterfly valve and T). Furthermore, a reasonable relative error percentage of 9.73 %, 12.24 %, 19.88 %, 16.48 % and 19.32 % respectively, is observed. The latter can be attributed to the points at which the data are taken could be not adequate. With this in mind, the proposed system can be improved by changing the experimental set up or measurement instruments. However, this work can be employed as a useful instructive guideline for future

Research paper thumbnail of Marshall parameters for quality control of hot mix asphalt after pavement construction

Revista de la construcción, 2019

Highways have been and are built using Hot Mix Asphalt (HMA) as surface layer. The most common HM... more Highways have been and are built using Hot Mix Asphalt (HMA) as surface layer. The most common HMA design procedure is the Marshall Method, a method also used for quality control (QC) at the time of road construction. Due to several factors, sometimes it is not possible to sample the surface layer when constructed. When this occurs, QC is performed after construction by analyzing cores. Current regulations, however, fail having clear procedures regarding the collection and analysis of pavement cores after the complete placement of the asphalt mixture. For this reason, this study proposes a comparative analysis of Marshall parameters, mixture densities, and aggregates gradation obtained from specimens obtained during construction, versus cores samples extracted 30 and 60 days later. The research revealed that all the parameters measured on core samples, except flow, could be used to approve asphalt paving works after its construction.

Research paper thumbnail of Diversity of genomic variants and population genetics of ethnic and regional groups across Russia

импозиум «Биоразнообразие: геномика и эволюция», 2018

Research paper thumbnail of Abstracts from the 13th WINFOCUS World Congress on Ultrasound in Emergency & Critical Care

Critical ultrasound journal, Jan 21, 2017

Background: Though an extremely rare complication of arterial cannulation, the incidence of radia... more Background: Though an extremely rare complication of arterial cannulation, the incidence of radial pseudoaneurysm may increase with the growing use of extended radial artery access for coronary angiography. Case report: A 57 year-old female presented to the emergency department with painful swelling to the volar radial surface of her right wrist 5 days after a non-emergent transradial coronary angiography. An emergency physician used point-of-care ultrasound to diagnose a radial artery pseudoaneurysm. The high-frequency linear transducer allowed visualization of the arterial wall defect and connection between artery and hematoma on B-mode, turbulent pulsatile flow into the adjacent hematoma using color flow Doppler, and a to-and-fro waveform at the wall defect using spectral Doppler. Due to the size and characteristics of the pseudoaneurysm, as well as her pain and mild distal sensory deficits, it was determined that the patient required prompt operative repair. Discussion: Bedside ultrasound is the most rapid and dynamic imaging modality for making diagnosing a radial artery pseudoaneurysm. Different techniques for treating this condition include conservative care, extended compression, thrombin injection, and surgery. Management primarily depends on the size of the pseudoaneurysm and its associated symptoms. Conclusion: In addition to understanding the pathophysiology and risk factors for this condition, the emergency physician must be adept at using point-of-care ultrasound to both make the diagnosis and characterize its findings to determine management. Consent for publication: The authors confirm that written informed consent was obtained for publication. A2 Type A thoracic aortic dissection suspected on resident performed bedside transthoracic echocardiography (TTE) in a patient with initial electrocardiogram (ECG) demonstrating an ST elevation myocardial infarction (STEMI)

Research paper thumbnail of Análise Comparativa Entre a Modalidade Cold-Wire e Hot- Wire No Processo De Soldagem Tig

Anais do IX Congresso Brasileiro de Engenharia de Fabricação, 2017

Research paper thumbnail of Testosterone increases CCL-2 expression in visceral adipose tissue from obese women of reproductive age

Molecular and cellular endocrinology, Mar 15, 2017

Hyperandrogenic states and obesity in women are associated with insulin-resistance. Androgens red... more Hyperandrogenic states and obesity in women are associated with insulin-resistance. Androgens reduce glucose uptake in adipose cells and increase TNFα production in peripheral monocytes. Inflammatory cytokines have a known detrimental effect on insulin resistance. The aim of the present study was to explore the role of testosterone in local cytokine production in visceral adipose tissue from women of reproductive age. Twenty-four women 18-40 years old, undergoing elective abdominal surgery for benign and non-inflammatory conditions, were recruited for the study. Women with clinical hyperandrogenism, diabetes, hepatic or renal dysfunction, hypothyroidism, BMI> 40 or drugs known to interfere with hormonal levels or fat metabolism were excluded. Women were classified into two groups according to BMI, non-obese (NO; BMI < 30) and obese (O; BMI 30-40). A basal blood sample was drawn at the time of surgery for the measurement of glucose, insulin, total testosterone, lipid profile an...