dipak singha - Academia.edu (original) (raw)

Papers by dipak singha

Asian Journal of Pharmaceutical Research and Development

Drug discovery and development is a time taking process, it has high risk also. Sometimes it get ... more Drug discovery and development is a time taking process, it has high risk also. Sometimes it get positive result, sometimes not. To discover, development and bring it into the market; pharmaceuticals companies invest millions of money. From drug discovery to bring it into the market these is a step by step procedure, it requires proper human resources, updated technological support and huge investment. A convenient surveillance is needed into the whole process from discovery to marketization that new entity. So these are the reasons for increasing cost in new drug development and research. But in recent time bioinformatics and pharmacogenomics made a huge impact on new drug development. Its helps in drug target identification, drug designing, clinical trial, not only bioinformatics and pharmacogenomics reduces the cost in healthcare management but also improves the selection of proper effective therapy and development of personalized medicines.

Acanthus ilicifolius plant extract prevents DNA alterations in a transplantable Ehrlich ascites c... more Acanthus ilicifolius plant extract prevents DNA alterations in a transplantable Ehrlich ascites carcinoma-bearing murine model

Acta Scientific Microbiology, 2022

The word Transgenic stands for one or more DNA sequences from another species have been introduce... more The word Transgenic stands for one or more DNA sequences from another species have been introduced by artificial ways. Transgenic animals are used as biomedical models in laboratory research, like rodent species. Rodent species which are important tools for researching human disease. These transgenic animals easily understands gene function in case of any disease and helps to determine the disease. Transgenic animals helps to produce complex human proteins in very large quantities. Transgenic animals have foreign genes in their genome and for this reason they can show some special properties other than normal animals. These properties lead to discover new drugs, helps in gene therapy etc. In case of gene therapy as defected genes could not function, so healthy genes are inserted by the help of vectors in th human body. Other than this, transgenic animals help in xerotransplantation. Pig is the experimental animal in case of xerotransplantation. Transgenic animals also play important role in agricultural field. Amount of milk with good quality and healthy meat have increased and farmers get benefited due to transgenic animals. These genetically modified animals are disease resistant and used in industrial purposes also.

Progress in Biomaterials, 2019

A hybrid hydrogel was obtained from decellularized extract from Wharton's jelly (DEWJ) and silk f... more A hybrid hydrogel was obtained from decellularized extract from Wharton's jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton's jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton's jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β1 (TGF-β1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications.

Rimonabant is an antiobesity drug which is CB1 cannabinoid receptor antagonist. Its main avenue o... more Rimonabant is an antiobesity drug which is CB1 cannabinoid receptor antagonist. Its main avenue of effect is reduction in appetite. So far only HPLC methods of analysis exist for the routine examination of drug. An attempt is hereby made to develop a simple spectrophotometric method for determination of drug from tablet dosage form. Rimonabant shows a maximum absorbance at 230 nm. Beer’s law was obeyed in the concentration range of 10-25 µg/ml. The method was validated statistically and recovery studies carried out.

Therapeutic protein are one of the prime option of biologicals as per their clinical uses. In rec... more Therapeutic protein are one of the prime option of biologicals as per their clinical uses. In recent times, uses of therapeutic protein increases day by day. Protein therapeutics are used extensively to treat various diseases like cancer, AIDS etc. Due to recent advancement in pharmaceutical biotechnology the interest towards therapeutic proteins are augmenting nowadays. Various clinical research are going on in this field to treat different diseases and pharmaceutical industries are also make interest on therapeutic proteins. Among the various treatment options therapeutic protein will provide highest chance of clinical success. Some recent clinical trials demonstrate that therapeutic protein may provide the safe and potential option to treat various diseases, but there are some drawbacks also like some immunogenic issues, safety, stability problem of protein, degradation of protein in various conditions.

World Journal of Gastroenterology, 2007

AIM: To investigate the chemopreventive effi cacy of the Indian medicinal plant Acanthus ilicifol... more AIM: To investigate the chemopreventive effi cacy of the Indian medicinal plant Acanthus ilicifolius L Acanthaceae in a transplantable Ehrlich ascites carcinoma (EAC)bearing murine model. METHODS: Male Swiss albino mice were divided into four groups: Group A was the untreated normal control; Group B was the EAC control mice group that received serial, intraperitoneal (ip) inoculations of rapidly proliferating 2 x 10 5 viable EAC cells in 0.2 mL of sterile phosphate buffered saline; Group C was the plant extract-treated group that received the aqueous leaf extract (ALE) of the plant at a dose of 2.5 mg/kg body weight by single ip injections, once daily for 10, 20 and 30 consecutive days following tumour inoculation (ALE control); and Group D was the EAC + ALEtreatment group. The chemopreventive potential of the ALE was evaluated in a murine model by studying various biological parameters and genotoxic markers, such as tumour cell count, mean survival of the animals, haematological indices, hepatocellular histology, immunohistochemical expression of liver metallothionein (MT) protein, sister-chromatid exchanges (SCEs), and DNA alterations. RESULTS: Treatment of the EAC-bearing mice with the ALE signifi cantly (P < 0.001) reduced viable tumour cell count by 68.34% (228.7 x 10 6 ± 0.53) when compared to EAC control mice (72.4 x 10 6 ± 0.49), and restored body and organ weights almost to the normal values. ALE administration also increased (P < 0.001) mean survival of the hosts from 35 ± 3.46 d in EAC control mice to 83 ± 2.69 d in EAC + ALE-treated mice. Haematological indices also showed marked improvement with administration of ALE in EAC-bearing animals. There was a significant increase in RBC count (P < 0.001), hemoglobin percent (P < 0.001), and haematocrit value (P < 0.001) from 4.3 ± 0.12, 6.4 ± 0.93, and 17.63 ± 0.72 respectively in EAC control mice to 7.1 ± 0.13, 12.1 ± 0.77, and 30.23 ± 0.57 respectively in EAC + ALE-treated group, along with concurrent decrement (P < 0.001) in WBC count from 18.8 ± 0.54 in EAC control to 8.4 ± 0.71 in EAC + ALE. Furthermore, treatment with ALE substantially improved hepatocellular architecture and no noticeable neoplastic lesions or foci of cellular alteration were observed. Daily administration of the ALE was found to limit liver MT expression, an important marker of cell proliferation with concomitant reduction in MT immunoreactivity (62.25 ± 2.58 vs 86.24 ± 5.69, P < 0.01). ALE was also potentially effective in reducing (P < 0.001) the frequency of SCEs from 14.94 ± 2.14 in EAC control to 5.12 ± 1.16 in EAC + ALE-treated group. Finally, in comparison to the EAC control, ALE was able to suppress in vivo DNA damage by abating the generations of 'tailed' DNA by 53.59% (98.65 ± 2.31 vs 45.06 ± 1.14, P < 0.001), and DNA single-strand breaks (SSBs) by 38.53% (3.14 ± 0.31 vs 1.93 ± 0.23, P < 0.01) in EAC-bearing murine liver. CONCLUSION: Our data indicate that, ALE is benefi cial in restoring haematological and hepatic histological profi les and in lengthening the survival of the animals against the proliferation of ascites tumour in vivo. Finally, the chemopreventive effi cacy of the ALE is manifested in limiting MT expression and in preventing DNA alterations in murine liver. The promising results of this study suggest further investigation into the chemopreventive mechanisms of the medicinal plant A. ilicifolius in vivo and in vitro .

World Journal of …, 2007

Tridib Chakraborty, Dipak Bhuniya, Mary Chatterjee, Mosiur Rahaman, Dipak Singha, Kartick Samanta... more Tridib Chakraborty, Dipak Bhuniya, Mary Chatterjee, Mosiur Rahaman, Dipak Singha, Kartick Samanta, Sunil Srivastawa, Malay Chatterjee, Chemical Carcinogenesis and Chemoprevention Laboratory, Division of Biochemistry, Department of Pharmaceutical Technology, ...

Asian Journal of Pharmaceutical Research and Development

Drug discovery and development is a time taking process, it has high risk also. Sometimes it get ... more Drug discovery and development is a time taking process, it has high risk also. Sometimes it get positive result, sometimes not. To discover, development and bring it into the market; pharmaceuticals companies invest millions of money. From drug discovery to bring it into the market these is a step by step procedure, it requires proper human resources, updated technological support and huge investment. A convenient surveillance is needed into the whole process from discovery to marketization that new entity. So these are the reasons for increasing cost in new drug development and research. But in recent time bioinformatics and pharmacogenomics made a huge impact on new drug development. Its helps in drug target identification, drug designing, clinical trial, not only bioinformatics and pharmacogenomics reduces the cost in healthcare management but also improves the selection of proper effective therapy and development of personalized medicines.

Acanthus ilicifolius plant extract prevents DNA alterations in a transplantable Ehrlich ascites c... more Acanthus ilicifolius plant extract prevents DNA alterations in a transplantable Ehrlich ascites carcinoma-bearing murine model

Acta Scientific Microbiology, 2022

The word Transgenic stands for one or more DNA sequences from another species have been introduce... more The word Transgenic stands for one or more DNA sequences from another species have been introduced by artificial ways. Transgenic animals are used as biomedical models in laboratory research, like rodent species. Rodent species which are important tools for researching human disease. These transgenic animals easily understands gene function in case of any disease and helps to determine the disease. Transgenic animals helps to produce complex human proteins in very large quantities. Transgenic animals have foreign genes in their genome and for this reason they can show some special properties other than normal animals. These properties lead to discover new drugs, helps in gene therapy etc. In case of gene therapy as defected genes could not function, so healthy genes are inserted by the help of vectors in th human body. Other than this, transgenic animals help in xerotransplantation. Pig is the experimental animal in case of xerotransplantation. Transgenic animals also play important role in agricultural field. Amount of milk with good quality and healthy meat have increased and farmers get benefited due to transgenic animals. These genetically modified animals are disease resistant and used in industrial purposes also.

Progress in Biomaterials, 2019

A hybrid hydrogel was obtained from decellularized extract from Wharton's jelly (DEWJ) and silk f... more A hybrid hydrogel was obtained from decellularized extract from Wharton's jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton's jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton's jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β1 (TGF-β1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications.

Rimonabant is an antiobesity drug which is CB1 cannabinoid receptor antagonist. Its main avenue o... more Rimonabant is an antiobesity drug which is CB1 cannabinoid receptor antagonist. Its main avenue of effect is reduction in appetite. So far only HPLC methods of analysis exist for the routine examination of drug. An attempt is hereby made to develop a simple spectrophotometric method for determination of drug from tablet dosage form. Rimonabant shows a maximum absorbance at 230 nm. Beer’s law was obeyed in the concentration range of 10-25 µg/ml. The method was validated statistically and recovery studies carried out.

Therapeutic protein are one of the prime option of biologicals as per their clinical uses. In rec... more Therapeutic protein are one of the prime option of biologicals as per their clinical uses. In recent times, uses of therapeutic protein increases day by day. Protein therapeutics are used extensively to treat various diseases like cancer, AIDS etc. Due to recent advancement in pharmaceutical biotechnology the interest towards therapeutic proteins are augmenting nowadays. Various clinical research are going on in this field to treat different diseases and pharmaceutical industries are also make interest on therapeutic proteins. Among the various treatment options therapeutic protein will provide highest chance of clinical success. Some recent clinical trials demonstrate that therapeutic protein may provide the safe and potential option to treat various diseases, but there are some drawbacks also like some immunogenic issues, safety, stability problem of protein, degradation of protein in various conditions.

World Journal of Gastroenterology, 2007

AIM: To investigate the chemopreventive effi cacy of the Indian medicinal plant Acanthus ilicifol... more AIM: To investigate the chemopreventive effi cacy of the Indian medicinal plant Acanthus ilicifolius L Acanthaceae in a transplantable Ehrlich ascites carcinoma (EAC)bearing murine model. METHODS: Male Swiss albino mice were divided into four groups: Group A was the untreated normal control; Group B was the EAC control mice group that received serial, intraperitoneal (ip) inoculations of rapidly proliferating 2 x 10 5 viable EAC cells in 0.2 mL of sterile phosphate buffered saline; Group C was the plant extract-treated group that received the aqueous leaf extract (ALE) of the plant at a dose of 2.5 mg/kg body weight by single ip injections, once daily for 10, 20 and 30 consecutive days following tumour inoculation (ALE control); and Group D was the EAC + ALEtreatment group. The chemopreventive potential of the ALE was evaluated in a murine model by studying various biological parameters and genotoxic markers, such as tumour cell count, mean survival of the animals, haematological indices, hepatocellular histology, immunohistochemical expression of liver metallothionein (MT) protein, sister-chromatid exchanges (SCEs), and DNA alterations. RESULTS: Treatment of the EAC-bearing mice with the ALE signifi cantly (P < 0.001) reduced viable tumour cell count by 68.34% (228.7 x 10 6 ± 0.53) when compared to EAC control mice (72.4 x 10 6 ± 0.49), and restored body and organ weights almost to the normal values. ALE administration also increased (P < 0.001) mean survival of the hosts from 35 ± 3.46 d in EAC control mice to 83 ± 2.69 d in EAC + ALE-treated mice. Haematological indices also showed marked improvement with administration of ALE in EAC-bearing animals. There was a significant increase in RBC count (P < 0.001), hemoglobin percent (P < 0.001), and haematocrit value (P < 0.001) from 4.3 ± 0.12, 6.4 ± 0.93, and 17.63 ± 0.72 respectively in EAC control mice to 7.1 ± 0.13, 12.1 ± 0.77, and 30.23 ± 0.57 respectively in EAC + ALE-treated group, along with concurrent decrement (P < 0.001) in WBC count from 18.8 ± 0.54 in EAC control to 8.4 ± 0.71 in EAC + ALE. Furthermore, treatment with ALE substantially improved hepatocellular architecture and no noticeable neoplastic lesions or foci of cellular alteration were observed. Daily administration of the ALE was found to limit liver MT expression, an important marker of cell proliferation with concomitant reduction in MT immunoreactivity (62.25 ± 2.58 vs 86.24 ± 5.69, P < 0.01). ALE was also potentially effective in reducing (P < 0.001) the frequency of SCEs from 14.94 ± 2.14 in EAC control to 5.12 ± 1.16 in EAC + ALE-treated group. Finally, in comparison to the EAC control, ALE was able to suppress in vivo DNA damage by abating the generations of 'tailed' DNA by 53.59% (98.65 ± 2.31 vs 45.06 ± 1.14, P < 0.001), and DNA single-strand breaks (SSBs) by 38.53% (3.14 ± 0.31 vs 1.93 ± 0.23, P < 0.01) in EAC-bearing murine liver. CONCLUSION: Our data indicate that, ALE is benefi cial in restoring haematological and hepatic histological profi les and in lengthening the survival of the animals against the proliferation of ascites tumour in vivo. Finally, the chemopreventive effi cacy of the ALE is manifested in limiting MT expression and in preventing DNA alterations in murine liver. The promising results of this study suggest further investigation into the chemopreventive mechanisms of the medicinal plant A. ilicifolius in vivo and in vitro .

World Journal of …, 2007

Tridib Chakraborty, Dipak Bhuniya, Mary Chatterjee, Mosiur Rahaman, Dipak Singha, Kartick Samanta... more Tridib Chakraborty, Dipak Bhuniya, Mary Chatterjee, Mosiur Rahaman, Dipak Singha, Kartick Samanta, Sunil Srivastawa, Malay Chatterjee, Chemical Carcinogenesis and Chemoprevention Laboratory, Division of Biochemistry, Department of Pharmaceutical Technology, ...