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Research paper thumbnail of Reduction of Target Volume Post Induction Chemotherapy Using Pet CT in Locally Advanced HNSCC

Research paper thumbnail of 3D printed phantom development and first approach for verification of individual dose in radiotherapy

Research paper thumbnail of Target volume delineation after induction chemotherapy in locally advanced head and neck cancer using PET/CT: is it possible to reduce the target volume?

Research paper thumbnail of RAS šeimos onkogenų mutacijų paplitimo lietuvos populiacijoje ir jų reikšmės išgyvenamumui, sergant metastazavusiu gaubtinės ar tiesiosios žarnos vėžiu, tyrimas

Research paper thumbnail of The Effect of signal - inducing proliferation - associated gene polymorphisms on breast cancer outcomes

Research paper thumbnail of sj-pdf-1-jbm-10.1177_17246008211011177 – Supplemental material for The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival

Supplemental material, sj-pdf-1-jbm-10.1177_17246008211011177 for The role of functional polymorp... more Supplemental material, sj-pdf-1-jbm-10.1177_17246008211011177 for The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival by Erika Korobeinikova, Rasa Ugenskiene, Ruta Insodaite, Viktoras Rudzianskas, Jurgita Gudaitiene and Elona Juozaityte in The International Journal of Biological Markers

Research paper thumbnail of The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival

The International Journal of Biological Markers

Background: Genetic variations in oxidative stress-related genes may alter the coded protein leve... more Background: Genetic variations in oxidative stress-related genes may alter the coded protein level and impact the pathogenesis of breast cancer. Methods: The current study investigated the associations of functional single nucleotide polymorphisms in the NFE2L2, HMOX1, P21, TXNRD2, and ATF3 genes with the early-stage breast cancer clinicopathological characteristics and disease-free survival, metastasis-free survival, and overall survival. A total of 202 Eastern European (Lithuanian) women with primary I–II stage breast cancer were involved. Genotyping of the single nucleotide polymorphisms was performed using TaqMan single nucleotide polymorphisms genotyping assays. Results: The CA+AA genotypes of P21 rs1801270 were significantly less frequent in patients with lymph node metastasis and larger tumor size ( P=0.041 and P=0.022, respectively). The TT genotype in ATF3 rs3125289 had significantly lower risk of estrogen receptor (ER), progesterone receptor (PR) negative, and human epider...

Research paper thumbnail of RESEARCH ARTICLE Open Access The

prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage brea... more prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage breast cancer patients

Research paper thumbnail of The Investigation of Associations between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344, EGFR rs2227983 Polymorphisms and Breast Cancer Phenotype and Prognosis

Diagnostics, 2021

Breast cancer is one of the most common oncological diseases among women worldwide. Cell cycle an... more Breast cancer is one of the most common oncological diseases among women worldwide. Cell cycle and apoptosis—related genes TP53, BBC3, CCND1 and EGFR play an important role in the pathogenesis of breast cancer. However, the roles of single nucleotide polymorphisms (SNPs) in these genes have not been fully defined. Therefore, this study aimed to analyze the association between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344 and EGFR rs2227983 polymorphisms and breast cancer phenotype and prognosis. For the purpose of the analysis, 171 Lithuanian women were enrolled. Genomic DNA was extracted from peripheral blood; PCR-RFLP was used for SNPs analysis. The results showed that BBC3 rs2032809 was associated with age at the time of diagnosis, disease progression, metastasis and death. CCND1 rs9344 was associated with tumor size, however an association resulted in loss of significance after Bonferroni correction. In survival analysis, significant associations were observed between BBC3 rs2032...

Research paper thumbnail of VEGFA gene polymorphism predict locoregional recurrence-free survival in early stage breast cancer patients

Research paper thumbnail of Application of polymer dose gels for millimeter scale target/tumor pre-treatment immitation using gamma knife facility

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms

Abstract Polymer gels are widely used for absorbed dose verification in radiotherapy, providing i... more Abstract Polymer gels are widely used for absorbed dose verification in radiotherapy, providing information about 3D dose distribution in the irradiated volume as well as serving as a simulation phantom, for the observation of radiation induced structural changes, related to gel’s polymerization upon irradiation. Due to high spatial resolution and unable sensitivity through gel’s composition, dosimeter gels is a powerful and precise tool for verification of individual treatment plans especially when high treatment doses are prescribed. The aim of this work was to modify and characterize gel dosimeters that are capable of millimetre scale target/tumour imitation and high dose assessment in small irradiated volume, for their further applications in clinical dosimetry and verification of millimetre scale target treatment procedures using Gamma knife unit.

Research paper thumbnail of Association of angiogenesis and inflammation‑related gene functional polymorphisms with early‑stage breast cancer prognosis

Oncology Letters

Genetic variations in inflammation-and angiogenesis-related genes may alter the coded protein lev... more Genetic variations in inflammation-and angiogenesis-related genes may alter the coded protein level and impact the pathogenesis of breast cancer (BC). The present study investigated the association of functional single nucleotide polymorphisms (SNPs) in the VEGFA, IL-1β, IL-1α and IL-6 genes with the early-stage BC phenotype and survival. Genomic DNA and clinical data were collected for 202 adult Eastern European (Lithuanian) women with primary I-II stage BC. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Nine VEGFA, IL-1β, IL-1α and IL-6 polymorphisms were analysed. The VEGFA and IL-6 haplotypes were inferred using Phase software. Patients were prospectively followed-up for recurrence, occurrence of metastasis and mortality until April 30, 2019. All studied genotypes were in Hardy-Weinberg equilibrium and had the same distribution as the 1,000 Genomes project Phase 3 dataset for European population. Significant associations of the studied SNPs with clinicopathologic variables were observed between IL-1α rs1800587 C allele and larger primary tumour size; IL-6 rs1800797 A allele, rs1800797 GA genotype, rs1800795 C allele, IL-6 (rs1800797-re1800795) AC diplotype and hormonal receptor-positive disease; IL-6 rs1800797 A allele and HER2 negative status. In univariate Cox survival analysis, IL-1α rs1800587 CC and IL-6 rs1800797 GG genotype carriers exhibited worse disease-free survival (DFS), metastasis-free survival (MFS) and overall survival (OS). The IL-6 rs1800795 GG genotype was associated with worse OS. IL-6 (rs1800797, rs1800795) GG/GG diplotype carriers had shorter MFS and OS. Multivariate Cox survival analysis revealed that the IL-1α rs1800587 CC genotype was an independent negative prognostic factor for DFS, MFS and OS, and the IL6 GG/GG diplotype was an independent negative prognostic factor for MFS and OS. According to the present study, functional SNPs in the IL-1α and IL-6 genes may contribute to the identification of patients at higher risk of BC recurrence, development of metastases and worse OS among early-stage patients with BC.

Research paper thumbnail of The impact of mitochondria-related POLG and TFAM variants on breast cancer pathomorphological characteristics and patient outcomes

Biomarkers

Abstract Purpose Breast cancer is the most frequent female cancer, leading to relapse with distan... more Abstract Purpose Breast cancer is the most frequent female cancer, leading to relapse with distant metastasis of approximately one-third of patients. Cancer is usually considered a genetic disease involving mutations in nuclear DNA. However, genes, coding for mitochondrial proteins or regulatory molecules, are rarely under consideration. This study aimed to analyse 10 single nucleotide variants in POLG and TFAM genes and assess their association with tumour phenotype and disease outcome. Materials and methods A total of 234 breast cancer patients were included in this study. Variations were determined with Real-Time PCR using TaqMan® probes. Results We found that patients with POLG rs2307441 TT and CT genotypes had a lower probability for vascular invasion than those with CC genotype (p = 0.001). Patients with POLG rs2072267 AG genotype were predisposed for progression compared with GG genotype (p = 0.015). TFAM rs3900887 TT genotype was associated with a higher probability for positive oestrogen receptors (p = 0.003) and lymphatic invasion (p = 0.001) in comparison to AA genotype, patients with TT (p = 0.000) were more likely to have positive lymph nodes. Conclusions Our data suggest that variations in POLG and TFAM genes are important determinacies of tumour phenotype and disease outcome in breast cancer patients.

Research paper thumbnail of Use of 18F-FDG PET/CT Imaging for Radiotherapy Target Volume Delineation after Induction Chemotherapy and for Prognosis of Locally Advanced Squamous Cell Carcinoma of the Head and Neck

Medicina

Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gi... more Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gives high response rates in locally advanced squamous cell carcinoma of the head and neck (LA-SCCHN). However, pre-ICT gross tumor volume (GTV) for radiotherapy (RT) planning is still recommended. As 18F-FDG PET/CT has an advantage of biological tumor information comparing to standard imaging methods, we aimed to evaluate the feasibility of 18F-FDG PET/CT-based post-ICT GTV delineation for RT planning in LA-SCCHN and to assess the prognostic value of PET parameters: maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion glycolysis (TLG). Methods: 47 LA-SCCHN patients were treated with 3 cycles of ICT (docetaxel, cisplatin, and 5-fluorouracil) followed by CRT (70 Gy in 35 fractions with weekly cisplatin). Pre- and post-ICT PET/CT examinations were acquired. Planning CT was co-registered with post-ICT PET/CT and RT target volumes were contoured according t...

Research paper thumbnail of Use of 18F-FDG PET/CT Imaging for Radiotherapy Target Volume Delineation after Induction Chemotherapy and for Prognosis of Locally Advanced Squamous Cell Carcinoma of the Head and Neck (LA-SCCHN)

Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gi... more Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gives high response rates in LA-SCCHN. However, pre-ICT gross tumour volume (GTV) for radiotherapy (RT) planning is still recommended. As 18 F-FDG PET/CT has an advantage of biological tumour information comparing to standard imaging methods, we aimed to evaluate the feasibility of 18 F-FDG PET/CT-based post-ICT GTV delineation for RT planning in LA-SCCHN and to assess the prognostic value of PET parameters: maximum standardized uptake value (SUVmax), metabolic tumour volume (MTV) and total lesion glycolysis (TLG). Methods: 47 LA-SCCHN patients were treated with 3 cycles of ICT (docetaxel, cisplatin, and 5-fluorouracil) followed by CRT (70 Gy in 35 fractions with weekly cisplatin). Pre-and post-ICT PET/CT examinations were acquired. Planning CT was co-registered with post-ICT PET/CT and RT target volumes were contoured according to post-ICT PET. Post-ICT percentage decrease of SUVmax, MTV and TLG in primary tumour and metastatic regional lymphnodes (LN) was counted. Loco-regional failure patterns, 3-year progression free (PFS) and overall survival (OS) were evaluated. Results: 3-year PFS and OS rates for study population were 67% and 61% respectively. 31.9% of patients progressed loco-regionally. All progresses were localised in high-to-intermediate dose (60-70Gy) RT volumes and none in low dose (50 Gy) volumes. Decrease of SUVmax ≥74% (p = 0.03), MTV ≥68% (p = 0.04), TLG ≥76% (p = 0.02) in primary tumour, and LN TLG decrease ≥74% (p = 0.03) were associated with PFS. Decrease of primary tumour SUVmax ≥74% (p = 0.04), MTV ≥69% (p = 0.04), TLG ≥74% (p = 0.02) and LN TLG ≥73% (p = 0.02) were prognostic factors for OS. Conclusions: According to our results, 18 F-FDG PET/CT-based post-ICT GTV delineation is feasible strategy without negative impact on loco-regional control and survival. Percentage decrease of metabolic PET parameters SUVmax, MTV and TLG has a prognostic value in LA-SCCHN.

Research paper thumbnail of Associations of MDM2 and MDM4 Polymorphisms with Early-Stage Breast Cancer

Journal of Clinical Medicine

Breast cancer is one of the most common cancers worldwide. Single nucleotide polymorphisms (SNPs)... more Breast cancer is one of the most common cancers worldwide. Single nucleotide polymorphisms (SNPs) in MDM2 and MDM4 have been associated with various cancers. However, the influence on clinical characteristics of breast cancer has not been sufficiently investigated yet. Thus, this study aimed to investigate the relationship between SNPs in MDM2 (rs2279744, rs937283, rs937282) and MDM4 (rs1380576, rs4245739) and I–II stage breast cancer. For analysis, the genomic DNA was extracted from 100 unrelated women peripheral blood. Polymorphisms were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The study showed that MDM2 rs937283 and rs937282 were significantly associated with estrogen receptor status and human epidermal growth factor receptor 2 (HER2) status. SNPs rs1380576 and rs4245739, located in MDM4, were significantly associated with status of estrogen and progesterone receptors. Our findings suggest that rs937283 AG, rs937282 CG, rs...

Research paper thumbnail of The prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage breast cancer patients

Research paper thumbnail of Reduction of Target Volume Post Induction Chemotherapy Using Pet CT in Locally Advanced HNSCC

Research paper thumbnail of 3D printed phantom development and first approach for verification of individual dose in radiotherapy

Research paper thumbnail of Target volume delineation after induction chemotherapy in locally advanced head and neck cancer using PET/CT: is it possible to reduce the target volume?

Research paper thumbnail of RAS šeimos onkogenų mutacijų paplitimo lietuvos populiacijoje ir jų reikšmės išgyvenamumui, sergant metastazavusiu gaubtinės ar tiesiosios žarnos vėžiu, tyrimas

Research paper thumbnail of The Effect of signal - inducing proliferation - associated gene polymorphisms on breast cancer outcomes

Research paper thumbnail of sj-pdf-1-jbm-10.1177_17246008211011177 – Supplemental material for The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival

Supplemental material, sj-pdf-1-jbm-10.1177_17246008211011177 for The role of functional polymorp... more Supplemental material, sj-pdf-1-jbm-10.1177_17246008211011177 for The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival by Erika Korobeinikova, Rasa Ugenskiene, Ruta Insodaite, Viktoras Rudzianskas, Jurgita Gudaitiene and Elona Juozaityte in The International Journal of Biological Markers

Research paper thumbnail of The role of functional polymorphisms in oxidative stress-related genes on early-stage breast cancer survival

The International Journal of Biological Markers

Background: Genetic variations in oxidative stress-related genes may alter the coded protein leve... more Background: Genetic variations in oxidative stress-related genes may alter the coded protein level and impact the pathogenesis of breast cancer. Methods: The current study investigated the associations of functional single nucleotide polymorphisms in the NFE2L2, HMOX1, P21, TXNRD2, and ATF3 genes with the early-stage breast cancer clinicopathological characteristics and disease-free survival, metastasis-free survival, and overall survival. A total of 202 Eastern European (Lithuanian) women with primary I–II stage breast cancer were involved. Genotyping of the single nucleotide polymorphisms was performed using TaqMan single nucleotide polymorphisms genotyping assays. Results: The CA+AA genotypes of P21 rs1801270 were significantly less frequent in patients with lymph node metastasis and larger tumor size ( P=0.041 and P=0.022, respectively). The TT genotype in ATF3 rs3125289 had significantly lower risk of estrogen receptor (ER), progesterone receptor (PR) negative, and human epider...

Research paper thumbnail of RESEARCH ARTICLE Open Access The

prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage brea... more prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage breast cancer patients

Research paper thumbnail of The Investigation of Associations between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344, EGFR rs2227983 Polymorphisms and Breast Cancer Phenotype and Prognosis

Diagnostics, 2021

Breast cancer is one of the most common oncological diseases among women worldwide. Cell cycle an... more Breast cancer is one of the most common oncological diseases among women worldwide. Cell cycle and apoptosis—related genes TP53, BBC3, CCND1 and EGFR play an important role in the pathogenesis of breast cancer. However, the roles of single nucleotide polymorphisms (SNPs) in these genes have not been fully defined. Therefore, this study aimed to analyze the association between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344 and EGFR rs2227983 polymorphisms and breast cancer phenotype and prognosis. For the purpose of the analysis, 171 Lithuanian women were enrolled. Genomic DNA was extracted from peripheral blood; PCR-RFLP was used for SNPs analysis. The results showed that BBC3 rs2032809 was associated with age at the time of diagnosis, disease progression, metastasis and death. CCND1 rs9344 was associated with tumor size, however an association resulted in loss of significance after Bonferroni correction. In survival analysis, significant associations were observed between BBC3 rs2032...

Research paper thumbnail of VEGFA gene polymorphism predict locoregional recurrence-free survival in early stage breast cancer patients

Research paper thumbnail of Application of polymer dose gels for millimeter scale target/tumor pre-treatment immitation using gamma knife facility

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms

Abstract Polymer gels are widely used for absorbed dose verification in radiotherapy, providing i... more Abstract Polymer gels are widely used for absorbed dose verification in radiotherapy, providing information about 3D dose distribution in the irradiated volume as well as serving as a simulation phantom, for the observation of radiation induced structural changes, related to gel’s polymerization upon irradiation. Due to high spatial resolution and unable sensitivity through gel’s composition, dosimeter gels is a powerful and precise tool for verification of individual treatment plans especially when high treatment doses are prescribed. The aim of this work was to modify and characterize gel dosimeters that are capable of millimetre scale target/tumour imitation and high dose assessment in small irradiated volume, for their further applications in clinical dosimetry and verification of millimetre scale target treatment procedures using Gamma knife unit.

Research paper thumbnail of Association of angiogenesis and inflammation‑related gene functional polymorphisms with early‑stage breast cancer prognosis

Oncology Letters

Genetic variations in inflammation-and angiogenesis-related genes may alter the coded protein lev... more Genetic variations in inflammation-and angiogenesis-related genes may alter the coded protein level and impact the pathogenesis of breast cancer (BC). The present study investigated the association of functional single nucleotide polymorphisms (SNPs) in the VEGFA, IL-1β, IL-1α and IL-6 genes with the early-stage BC phenotype and survival. Genomic DNA and clinical data were collected for 202 adult Eastern European (Lithuanian) women with primary I-II stage BC. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Nine VEGFA, IL-1β, IL-1α and IL-6 polymorphisms were analysed. The VEGFA and IL-6 haplotypes were inferred using Phase software. Patients were prospectively followed-up for recurrence, occurrence of metastasis and mortality until April 30, 2019. All studied genotypes were in Hardy-Weinberg equilibrium and had the same distribution as the 1,000 Genomes project Phase 3 dataset for European population. Significant associations of the studied SNPs with clinicopathologic variables were observed between IL-1α rs1800587 C allele and larger primary tumour size; IL-6 rs1800797 A allele, rs1800797 GA genotype, rs1800795 C allele, IL-6 (rs1800797-re1800795) AC diplotype and hormonal receptor-positive disease; IL-6 rs1800797 A allele and HER2 negative status. In univariate Cox survival analysis, IL-1α rs1800587 CC and IL-6 rs1800797 GG genotype carriers exhibited worse disease-free survival (DFS), metastasis-free survival (MFS) and overall survival (OS). The IL-6 rs1800795 GG genotype was associated with worse OS. IL-6 (rs1800797, rs1800795) GG/GG diplotype carriers had shorter MFS and OS. Multivariate Cox survival analysis revealed that the IL-1α rs1800587 CC genotype was an independent negative prognostic factor for DFS, MFS and OS, and the IL6 GG/GG diplotype was an independent negative prognostic factor for MFS and OS. According to the present study, functional SNPs in the IL-1α and IL-6 genes may contribute to the identification of patients at higher risk of BC recurrence, development of metastases and worse OS among early-stage patients with BC.

Research paper thumbnail of The impact of mitochondria-related POLG and TFAM variants on breast cancer pathomorphological characteristics and patient outcomes

Biomarkers

Abstract Purpose Breast cancer is the most frequent female cancer, leading to relapse with distan... more Abstract Purpose Breast cancer is the most frequent female cancer, leading to relapse with distant metastasis of approximately one-third of patients. Cancer is usually considered a genetic disease involving mutations in nuclear DNA. However, genes, coding for mitochondrial proteins or regulatory molecules, are rarely under consideration. This study aimed to analyse 10 single nucleotide variants in POLG and TFAM genes and assess their association with tumour phenotype and disease outcome. Materials and methods A total of 234 breast cancer patients were included in this study. Variations were determined with Real-Time PCR using TaqMan® probes. Results We found that patients with POLG rs2307441 TT and CT genotypes had a lower probability for vascular invasion than those with CC genotype (p = 0.001). Patients with POLG rs2072267 AG genotype were predisposed for progression compared with GG genotype (p = 0.015). TFAM rs3900887 TT genotype was associated with a higher probability for positive oestrogen receptors (p = 0.003) and lymphatic invasion (p = 0.001) in comparison to AA genotype, patients with TT (p = 0.000) were more likely to have positive lymph nodes. Conclusions Our data suggest that variations in POLG and TFAM genes are important determinacies of tumour phenotype and disease outcome in breast cancer patients.

Research paper thumbnail of Use of 18F-FDG PET/CT Imaging for Radiotherapy Target Volume Delineation after Induction Chemotherapy and for Prognosis of Locally Advanced Squamous Cell Carcinoma of the Head and Neck

Medicina

Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gi... more Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gives high response rates in locally advanced squamous cell carcinoma of the head and neck (LA-SCCHN). However, pre-ICT gross tumor volume (GTV) for radiotherapy (RT) planning is still recommended. As 18F-FDG PET/CT has an advantage of biological tumor information comparing to standard imaging methods, we aimed to evaluate the feasibility of 18F-FDG PET/CT-based post-ICT GTV delineation for RT planning in LA-SCCHN and to assess the prognostic value of PET parameters: maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion glycolysis (TLG). Methods: 47 LA-SCCHN patients were treated with 3 cycles of ICT (docetaxel, cisplatin, and 5-fluorouracil) followed by CRT (70 Gy in 35 fractions with weekly cisplatin). Pre- and post-ICT PET/CT examinations were acquired. Planning CT was co-registered with post-ICT PET/CT and RT target volumes were contoured according t...

Research paper thumbnail of Use of 18F-FDG PET/CT Imaging for Radiotherapy Target Volume Delineation after Induction Chemotherapy and for Prognosis of Locally Advanced Squamous Cell Carcinoma of the Head and Neck (LA-SCCHN)

Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gi... more Background and objectives: Induction chemotherapy (ICT) before definitive chemoradiation (CRT) gives high response rates in LA-SCCHN. However, pre-ICT gross tumour volume (GTV) for radiotherapy (RT) planning is still recommended. As 18 F-FDG PET/CT has an advantage of biological tumour information comparing to standard imaging methods, we aimed to evaluate the feasibility of 18 F-FDG PET/CT-based post-ICT GTV delineation for RT planning in LA-SCCHN and to assess the prognostic value of PET parameters: maximum standardized uptake value (SUVmax), metabolic tumour volume (MTV) and total lesion glycolysis (TLG). Methods: 47 LA-SCCHN patients were treated with 3 cycles of ICT (docetaxel, cisplatin, and 5-fluorouracil) followed by CRT (70 Gy in 35 fractions with weekly cisplatin). Pre-and post-ICT PET/CT examinations were acquired. Planning CT was co-registered with post-ICT PET/CT and RT target volumes were contoured according to post-ICT PET. Post-ICT percentage decrease of SUVmax, MTV and TLG in primary tumour and metastatic regional lymphnodes (LN) was counted. Loco-regional failure patterns, 3-year progression free (PFS) and overall survival (OS) were evaluated. Results: 3-year PFS and OS rates for study population were 67% and 61% respectively. 31.9% of patients progressed loco-regionally. All progresses were localised in high-to-intermediate dose (60-70Gy) RT volumes and none in low dose (50 Gy) volumes. Decrease of SUVmax ≥74% (p = 0.03), MTV ≥68% (p = 0.04), TLG ≥76% (p = 0.02) in primary tumour, and LN TLG decrease ≥74% (p = 0.03) were associated with PFS. Decrease of primary tumour SUVmax ≥74% (p = 0.04), MTV ≥69% (p = 0.04), TLG ≥74% (p = 0.02) and LN TLG ≥73% (p = 0.02) were prognostic factors for OS. Conclusions: According to our results, 18 F-FDG PET/CT-based post-ICT GTV delineation is feasible strategy without negative impact on loco-regional control and survival. Percentage decrease of metabolic PET parameters SUVmax, MTV and TLG has a prognostic value in LA-SCCHN.

Research paper thumbnail of Associations of MDM2 and MDM4 Polymorphisms with Early-Stage Breast Cancer

Journal of Clinical Medicine

Breast cancer is one of the most common cancers worldwide. Single nucleotide polymorphisms (SNPs)... more Breast cancer is one of the most common cancers worldwide. Single nucleotide polymorphisms (SNPs) in MDM2 and MDM4 have been associated with various cancers. However, the influence on clinical characteristics of breast cancer has not been sufficiently investigated yet. Thus, this study aimed to investigate the relationship between SNPs in MDM2 (rs2279744, rs937283, rs937282) and MDM4 (rs1380576, rs4245739) and I–II stage breast cancer. For analysis, the genomic DNA was extracted from 100 unrelated women peripheral blood. Polymorphisms were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The study showed that MDM2 rs937283 and rs937282 were significantly associated with estrogen receptor status and human epidermal growth factor receptor 2 (HER2) status. SNPs rs1380576 and rs4245739, located in MDM4, were significantly associated with status of estrogen and progesterone receptors. Our findings suggest that rs937283 AG, rs937282 CG, rs...

Research paper thumbnail of The prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage breast cancer patients