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Papers by Elahe Soltani Fard

Research paper thumbnail of Exosomal lncRNAs in gastrointestinal cancer

Research paper thumbnail of Nattokinase: Structure, applications and sources

Biocatalysis and Agricultural Biotechnology

Research paper thumbnail of Designing a Humanized Immunotoxin Based on HER2 Specific scFv and DFF40 Toxin Against Breast Cancer: An In-Silico Study

International Journal of Peptide Research and Therapeutics

Research paper thumbnail of Aptamer-based biosensors for Pseudomonas aeruginosa detection

Molecular and Cellular Probes

Research paper thumbnail of Insights into the Function of Regulatory RNAs in Bacteria and Archaea

International Journal of Translational Medicine

Non-coding RNAs (ncRNAs) are functional RNA molecules that comprise about 80% of both mammals and... more Non-coding RNAs (ncRNAs) are functional RNA molecules that comprise about 80% of both mammals and prokaryotes genomes. Recent studies have identified a large number of small regulatory RNAs in Escherichia coli and other bacteria. In prokaryotes, RNA regulators are a diverse group of molecules that modulate a wide range of physiological responses through a variety of mechanisms. Similar to eukaryotes, bacterial microRNAs are an important class of ncRNAs that play an important role in the development and secretion of proteins and in the regulation of gene expression. Similarly, riboswitches are cis-regulatory structured RNA elements capable of directly controlling the expression of downstream genes in response to small molecule ligands. As a result, riboswitches detect and respond to the availability of various metabolic changes within cells. The most extensive and most widely studied set of small RNA regulators act through base pairing with RNAs. These types of RNAs are vital for pro...

Research paper thumbnail of Exosomal microRNAs and long noncoding RNAs: Novel mediators of drug resistance in lung cancer

Journal of Cellular Physiology

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and

Research paper thumbnail of Molecular Diversity of Methicillin-resistant Staphylococcus aureus Isolates Originated from Patients in Ahvaz Hospitals, Iran

Introduction: Staphylococcus aureus is among the primary cause of hospitals and community-acquire... more Introduction: Staphylococcus aureus is among the primary cause of hospitals and community-acquired infections. The emergence of methicillin-resistant S. aureus (MRSA) strains has resulted in the treatment failure of the infections caused by these bacteria. Hence, regional data on antibiotic resistance of S. aureus strains is necessary to adopt appropriate treatment regimens. This study aims to identify MRSA isolates' diversities and frequencies by molecular analysis of four genes. Methods: In a crosssectional study, 100 S. aureus isolates from patients hospitalized in two hospitals of Ahvaz, Iran were collected and identified. The MRSA isolates were identified by phenotypic method and amplification of the mecA gene. The diversity of MRSA isolates was investigated by amplification of the coa, spa, aroA, and gap genes followed by RFLP analysis using the AluI, HindIII, TaqI and RsaI restriction enzymes. Results: In this study, we identified 50 MRSA isolates. Based on the analysis of coa gene, 8 types, spa gene 5 types and 17 subtypes, coa gene with AluI 13 types, and spa with HindIII 13 types were identified. Also, the RFLP analysis of gap gene with AluI revealed 3 types, and of aroA gene with TaqI and RsaI, 3 types and 2 subtypes, respectively. Conclusion: Our PCR-RFLP analysis revealed that diversities are present among MRSA isolates originated from clinical samples and showed that this method is simple, reproducible, and cost-effective.

Research paper thumbnail of Glucose oxidase: Applications, sources, and recombinant production

Biotechnology and Applied Biochemistry

Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons fr... more Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external electron acceptor that releases hydrogen peroxide (H2 O2 ). Glucose oxidase has many applications in commercial processes, including improving the color and taste, increasing the persistence of food materials, removing the glucose from the dried egg, and eliminating the oxygen from different juices and beverages. Moreover, glucose oxidase, along with catalase, is used in glucose testing kits (esp., in biosensors) to detect and measure the presence of glucose in industrial and biological solutions (e.g., blood and urine specimens). Hence, glucose oxidase is a valuable enzyme in the industry and medical diagnostics. Therefore, evaluating the structure and function of glucose oxidase is crucial for modifying as well as improving its catalytic properties. Finding different sources of glucose oxidase is an effective way ...

Research paper thumbnail of A review on medical plants affecting Staphylococcus aureus

Background and aims: Infectious diseases are among the most important causes of mortality around ... more Background and aims: Infectious diseases are among the most important causes of mortality around the world and according to the WHO report 10.5 million cases of death were happened due to infections. Staphylococcus aureus is one of the most important pathogen that its infections are varied from local infections to life threatening infections. With regard to high antibiotic resistance of this agent its control and treatment is a challenge in medicine. The aim of this study was to review the effective medicinal plants against S. aureus with special attention of native plants of Iran. Methods: A comprehensive literature review was performed on papers that have been published from 2004 till 2016 in data resources such as NCBI, Sciencedirect, Springer, Web of science and as well as local databases such as Irandoc, Islamic science citation (ISC) and magiran with special focus on those that have been reported native medicinal plants in Iran. The selected keywords were Staphylococcus aureus...

Research paper thumbnail of LncRNAs roles in Chemoresistance of Cancer cells

Current Molecular Medicine

: Cancer is an important health issue worldwide. Cancer therapy is multifaceted, and drug resista... more : Cancer is an important health issue worldwide. Cancer therapy is multifaceted, and drug resistance is still the major limiting factor in treatment of patients with this disease. Although the mechanisms of anticancer drug resistance have been broadly investigated, a massive biological signal pathway of Non-coding RNAs (ncRNAs) involved in this process has not been completely understood. Long noncoding RNAs (lncRNAs) are a kind of transcripts with a minimum length of 200 nucleotides in size which have a limited potential for coding proteins. The roles of these RNA molecules have been evaluated in relation to several pathological processes including tumor formation and progression. Increasing evidence haverecently reported that non-coding RNAs (ncRNAs), particularly long non-coding RNAs have significant roles in many cellular and genomic processes, and because of their potential in regulation specific genes, they are also involved in drug resistance. In this review, we review the literature on the features oflncRNA, their regulation roles in the gene expression related to chemoresistance and the potential of these RNAs as targeted therapies for personalized treatment in cancers.

Research paper thumbnail of Glucose oxidase: Applications, sources, and recombinant production

Biotechnology and Applied Biochemistry

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and

Research paper thumbnail of Exosomal lncRNAs in gastrointestinal cancer

Research paper thumbnail of Nattokinase: Structure, applications and sources

Biocatalysis and Agricultural Biotechnology

Research paper thumbnail of Designing a Humanized Immunotoxin Based on HER2 Specific scFv and DFF40 Toxin Against Breast Cancer: An In-Silico Study

International Journal of Peptide Research and Therapeutics

Research paper thumbnail of Aptamer-based biosensors for Pseudomonas aeruginosa detection

Molecular and Cellular Probes

Research paper thumbnail of Insights into the Function of Regulatory RNAs in Bacteria and Archaea

International Journal of Translational Medicine

Non-coding RNAs (ncRNAs) are functional RNA molecules that comprise about 80% of both mammals and... more Non-coding RNAs (ncRNAs) are functional RNA molecules that comprise about 80% of both mammals and prokaryotes genomes. Recent studies have identified a large number of small regulatory RNAs in Escherichia coli and other bacteria. In prokaryotes, RNA regulators are a diverse group of molecules that modulate a wide range of physiological responses through a variety of mechanisms. Similar to eukaryotes, bacterial microRNAs are an important class of ncRNAs that play an important role in the development and secretion of proteins and in the regulation of gene expression. Similarly, riboswitches are cis-regulatory structured RNA elements capable of directly controlling the expression of downstream genes in response to small molecule ligands. As a result, riboswitches detect and respond to the availability of various metabolic changes within cells. The most extensive and most widely studied set of small RNA regulators act through base pairing with RNAs. These types of RNAs are vital for pro...

Research paper thumbnail of Exosomal microRNAs and long noncoding RNAs: Novel mediators of drug resistance in lung cancer

Journal of Cellular Physiology

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and

Research paper thumbnail of Molecular Diversity of Methicillin-resistant Staphylococcus aureus Isolates Originated from Patients in Ahvaz Hospitals, Iran

Introduction: Staphylococcus aureus is among the primary cause of hospitals and community-acquire... more Introduction: Staphylococcus aureus is among the primary cause of hospitals and community-acquired infections. The emergence of methicillin-resistant S. aureus (MRSA) strains has resulted in the treatment failure of the infections caused by these bacteria. Hence, regional data on antibiotic resistance of S. aureus strains is necessary to adopt appropriate treatment regimens. This study aims to identify MRSA isolates' diversities and frequencies by molecular analysis of four genes. Methods: In a crosssectional study, 100 S. aureus isolates from patients hospitalized in two hospitals of Ahvaz, Iran were collected and identified. The MRSA isolates were identified by phenotypic method and amplification of the mecA gene. The diversity of MRSA isolates was investigated by amplification of the coa, spa, aroA, and gap genes followed by RFLP analysis using the AluI, HindIII, TaqI and RsaI restriction enzymes. Results: In this study, we identified 50 MRSA isolates. Based on the analysis of coa gene, 8 types, spa gene 5 types and 17 subtypes, coa gene with AluI 13 types, and spa with HindIII 13 types were identified. Also, the RFLP analysis of gap gene with AluI revealed 3 types, and of aroA gene with TaqI and RsaI, 3 types and 2 subtypes, respectively. Conclusion: Our PCR-RFLP analysis revealed that diversities are present among MRSA isolates originated from clinical samples and showed that this method is simple, reproducible, and cost-effective.

Research paper thumbnail of Glucose oxidase: Applications, sources, and recombinant production

Biotechnology and Applied Biochemistry

Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons fr... more Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external electron acceptor that releases hydrogen peroxide (H2 O2 ). Glucose oxidase has many applications in commercial processes, including improving the color and taste, increasing the persistence of food materials, removing the glucose from the dried egg, and eliminating the oxygen from different juices and beverages. Moreover, glucose oxidase, along with catalase, is used in glucose testing kits (esp., in biosensors) to detect and measure the presence of glucose in industrial and biological solutions (e.g., blood and urine specimens). Hence, glucose oxidase is a valuable enzyme in the industry and medical diagnostics. Therefore, evaluating the structure and function of glucose oxidase is crucial for modifying as well as improving its catalytic properties. Finding different sources of glucose oxidase is an effective way ...

Research paper thumbnail of A review on medical plants affecting Staphylococcus aureus

Background and aims: Infectious diseases are among the most important causes of mortality around ... more Background and aims: Infectious diseases are among the most important causes of mortality around the world and according to the WHO report 10.5 million cases of death were happened due to infections. Staphylococcus aureus is one of the most important pathogen that its infections are varied from local infections to life threatening infections. With regard to high antibiotic resistance of this agent its control and treatment is a challenge in medicine. The aim of this study was to review the effective medicinal plants against S. aureus with special attention of native plants of Iran. Methods: A comprehensive literature review was performed on papers that have been published from 2004 till 2016 in data resources such as NCBI, Sciencedirect, Springer, Web of science and as well as local databases such as Irandoc, Islamic science citation (ISC) and magiran with special focus on those that have been reported native medicinal plants in Iran. The selected keywords were Staphylococcus aureus...

Research paper thumbnail of LncRNAs roles in Chemoresistance of Cancer cells

Current Molecular Medicine

: Cancer is an important health issue worldwide. Cancer therapy is multifaceted, and drug resista... more : Cancer is an important health issue worldwide. Cancer therapy is multifaceted, and drug resistance is still the major limiting factor in treatment of patients with this disease. Although the mechanisms of anticancer drug resistance have been broadly investigated, a massive biological signal pathway of Non-coding RNAs (ncRNAs) involved in this process has not been completely understood. Long noncoding RNAs (lncRNAs) are a kind of transcripts with a minimum length of 200 nucleotides in size which have a limited potential for coding proteins. The roles of these RNA molecules have been evaluated in relation to several pathological processes including tumor formation and progression. Increasing evidence haverecently reported that non-coding RNAs (ncRNAs), particularly long non-coding RNAs have significant roles in many cellular and genomic processes, and because of their potential in regulation specific genes, they are also involved in drug resistance. In this review, we review the literature on the features oflncRNA, their regulation roles in the gene expression related to chemoresistance and the potential of these RNAs as targeted therapies for personalized treatment in cancers.

Research paper thumbnail of Glucose oxidase: Applications, sources, and recombinant production

Biotechnology and Applied Biochemistry

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and

Research paper thumbnail of SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Avicenna Journal of Clinical Microbiology and Infection

Background Staphylococcus aureus is a very important hospital and community-acquired pathogen tha... more Background Staphylococcus aureus is a very important hospital and community-acquired pathogen that causes extendedspectrum infections. The infectious potential is related to various bacterial surface cell components and extracellular secreting proteins (1). Various factors such as toxins, invasion and antibiotic resistance are involved in pathogenicity of this bacterium. Resistant strains against a wide variety of antimicrobial agents are emerging frequently due to expression of new resistance mechanisms by this pathogen (2,3). Methicillin resistant S. aureus (MRSA) strains are the main resistant strains of this pathogen around the world and vancomycin is the only choice to fight them. But unfortunately, vancomycin resistant strains are also growing among hospital and/or community-acquired infections (4). The mecA gene on the staphylococcal cassette chromosome mec (SCCmec) element is responsible for methicillin resistance (5). SCCmec genomic island contains mec gene complex and ccr (cassette chromosome recombinase) gene complex. Eleven major types (I-XI) are known for SCCmec elements some of which are divided into subtypes. In cases of hospital-acquired MRSA (HA-MRSA), more frequently detected SCCmec types have been I, II, and III, while in community-acquired MRSA (CA-MRSA), the types IV and V have been mostly involved (5). Detection of the SCCmec type of an isolate is necessary for control of hospital infections and prevention of the transmission of infections (6). The arginine catabolic mobile element (ACME) inhibits polymorphonuclear cell production and plays an important role in bacterial growth and survival, colonization in human skin and extensive dissemination. In MRSA strains, ACME is always integrated with SCCmec elements. SCCmec recombinase is likely to mediate its integration and excision (4). Physical relationship between SCCmec and ACME genes suggest that the pathogenicity and