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Papers by eman abdo

Journal of the Advances in Agricultural Researches

In the last decade, microbial polysaccharides have received increasing attention, and it has been... more In the last decade, microbial polysaccharides have received increasing attention, and it has been demonstrated that many microbial strains can produce polysaccharides with different compositions and functionality. The isolated and characterized Lactobacillus paracasei has been studied for its ability to produce exopolysaccharides (EPS). In addition, the effect of carbon sources on EPS production was investigated. The effect of Lactobacillus paracasei on the viscosity and sensory properties of yoghurt made from whole milk and skim milk was studied. Lactobacillus paracasei had excellent biomass (4.4 g/L) with good biomass separation (0.11). Using sucrose 100 g/L as a carbon source for 3 days gave the best EPS content (0.7 g/L). Infrared spectra showed that the EPS produced by Lactobacillus paracasei was a heteropolysaccharide. Fortification with Lactobacillus paracasei in the production of yoghurt from skim milk resulted in a relatively similar viscosity (5155.20 ± 4.53 mPa.S) to that of whole milk yoghurt (5264.30 ± 2.40 mPa.S), reflecting the improvement in the sensory characteristics of yoghurt from skim milk, especially mouthfeel. Consequently, fortification with Lactobacillus paracasei is recommended in low-fat yoghurt.

Frontiers in Nutrition

IntroductionAdequate protein and antioxidant intake are crucial for everyone, particularly athlet... more IntroductionAdequate protein and antioxidant intake are crucial for everyone, particularly athletes, to promote muscle performance and prevent muscle damage. Whey proteins are high-quality proteins with high digestibility and bioavailability; beetroot peels are an abundant antioxidant source.MethodsThe present study was designated to develop a functional beverage based on mixing whey protein isolate (5%) with different concentrations of beetroot peel water extract (1, 2.5, and 5%) and flavored with strawberries puree (5%). In addition, we examined the stability of the physicochemical parameters and the bioactive components of the beverages during cold storage (4°C) for 14 days.Results and discussionWhey protein isolates enriched the juices with stable protein content during the storage (4.65–4.69%). Besides, the extract revealed a concentration-dependent effect on the bioactive components, the antioxidant activity, and the microbial load of the juices; it distinguished the fresh jui...

Antioxidants

Plant by-products are safe, sustainable, and abundant natural antioxidant sources. Here we invest... more Plant by-products are safe, sustainable, and abundant natural antioxidant sources. Here we investigated the antioxidant activity of a mixture of lyophilized pomegranate, guava, and grape (PGG) leaves water extract (1:1:1) and examined its ability to retard the rancidity of soybean oil during accelerated storage at 65 °C for 30 days. To achieve this, we evaluated the oxidative stability of soybean oil enriched with PGG extract at 200, 400, and 800 ppm. We also compared the effect of PGG extract with butylated hydroxytoluene (BHT) (400/100 ppm) with that of only BHT (200 ppm). We observed that 8.19 and 1.78 µg/mL of the extract could scavenge 50% of DPPH• and ABTS•, respectively, indicating its enhanced antioxidant activity. Enriching soyabean oil with the extract at 800 ppm improved its oxidative stability by reducing the acid value to 1.71 mg/g and the total oxidation to 99.87 compared to 2.27 mg/g and 150.32 in the raw oil, respectively. Moreover, PGG-800 ppm inhibited oxidation by...

Antioxidants, 2022

Obesity is one of the most prevalent non-communicable diseases and is interlinked with incidences... more Obesity is one of the most prevalent non-communicable diseases and is interlinked with incidences of various diseases. By modulating lifestyle and food quality, obesity can be preventable. The present study investigated the ability of a novel functional beverage based on orange juice and beetroot leaf and stem juice in preventing obesity-associated health issues. To achieve this purpose, we determined the nutritive value of juices and tested their ability to prevent the effect of a high-fat diet on a rat model. Adding leaf and stem juice to orange juice increased the total soluble solids/total titratable acidity ratio, which reflects the high acceptability of the blends, and enhanced their nutritive value. All minerals increased in the blends by increasing the leaf and stem juice percentage. Copper was detected only in the juices containing 10–20% leaf and stem juice (0.01–0.11 mg/100 g). Total flavonoids and betalain increased in the blends, reaching 142.02 µg/mL and 1680 µg/mL, re...

Journal of Functional Foods, 2021

Abstract The present study incorporated beetroot pomace powder in biscuit preparation at concentr... more Abstract The present study incorporated beetroot pomace powder in biscuit preparation at concentrations of 5–20%. Biscuits' chemical composition, and sensory were evaluated. A feeding experiment was conducted using three groups (n = 3); control and anemic (An+) groups fed a standard diet, and anemic group fed 90% standard-diet and 10% biscuit containing 15% pomace (AnB). Anemia induced by injecting rats with phenylhydrazine (40 mg/kg) for two consecutive days. Hematological and biochemical blood parameters were determined at days 3, 14, and 28. An enhancement in protein, fiber, calcium, phosphorus, and iron was observed in the biscuit by increasing the pomace. Consuming biscuits containing 15% pomace increased hemoglobin, RBCs, and antioxidant enzymes in (AnB) rats after 28 days. The minor kidney and liver dysfunction in the anemic groups were recovered in (AnB) after 28 days. Consequently, incorporation beet pomace in biscuit revealed a promising role in anemia treatment and oxidative stress managing.

Food and Nutrition Sciences, 2014

Bt corn "MON810: Ajeeb YG" produces delta endotoxins in the whole plant due to the genetic modifi... more Bt corn "MON810: Ajeeb YG" produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart "Ajeeb". Further, feeding studies on rats were designed to complete assessing the safety of "MON810: Ajeeb YG". Three groups of rats (6 males and 6 females' rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.

Plants, 2020

Beetroot is a good source of minerals, fibers, and bioactive components. The present research wor... more Beetroot is a good source of minerals, fibers, and bioactive components. The present research work was conducted to evaluate the nutritional quality of beetroots (juice, peels, leaves and pomace) enhancing the extracted bioactive components, and developing a functional probiotic beverage. Chemical composition and minerals content of beetroot parts were estimated. The bioactive components were extracted by instant extraction method (IEM) and overnight extraction method (at −20 °C) (OEM) to determine total phenolics, flavonoids, and DPPH inhibition ratio. The extracted beetroot juice was mixed with milk for valorization of the beverage nutritional value and fermented with LA-5 and ABT-5 cultures to create a novel functional beverage. Chemical composition, minerals content, and bioactive components of beverages were estimated. The leaves exhibited the highest calcium content (1200 mg/100 g). Juice showed the highest amount of all minerals except for calcium and magnesium. Overnight ext...

Journal of the Advances in Agricultural Researches

In the last decade, microbial polysaccharides have received increasing attention, and it has been... more In the last decade, microbial polysaccharides have received increasing attention, and it has been demonstrated that many microbial strains can produce polysaccharides with different compositions and functionality. The isolated and characterized Lactobacillus paracasei has been studied for its ability to produce exopolysaccharides (EPS). In addition, the effect of carbon sources on EPS production was investigated. The effect of Lactobacillus paracasei on the viscosity and sensory properties of yoghurt made from whole milk and skim milk was studied. Lactobacillus paracasei had excellent biomass (4.4 g/L) with good biomass separation (0.11). Using sucrose 100 g/L as a carbon source for 3 days gave the best EPS content (0.7 g/L). Infrared spectra showed that the EPS produced by Lactobacillus paracasei was a heteropolysaccharide. Fortification with Lactobacillus paracasei in the production of yoghurt from skim milk resulted in a relatively similar viscosity (5155.20 ± 4.53 mPa.S) to that of whole milk yoghurt (5264.30 ± 2.40 mPa.S), reflecting the improvement in the sensory characteristics of yoghurt from skim milk, especially mouthfeel. Consequently, fortification with Lactobacillus paracasei is recommended in low-fat yoghurt.

Frontiers in Nutrition

IntroductionAdequate protein and antioxidant intake are crucial for everyone, particularly athlet... more IntroductionAdequate protein and antioxidant intake are crucial for everyone, particularly athletes, to promote muscle performance and prevent muscle damage. Whey proteins are high-quality proteins with high digestibility and bioavailability; beetroot peels are an abundant antioxidant source.MethodsThe present study was designated to develop a functional beverage based on mixing whey protein isolate (5%) with different concentrations of beetroot peel water extract (1, 2.5, and 5%) and flavored with strawberries puree (5%). In addition, we examined the stability of the physicochemical parameters and the bioactive components of the beverages during cold storage (4°C) for 14 days.Results and discussionWhey protein isolates enriched the juices with stable protein content during the storage (4.65–4.69%). Besides, the extract revealed a concentration-dependent effect on the bioactive components, the antioxidant activity, and the microbial load of the juices; it distinguished the fresh jui...

Antioxidants

Plant by-products are safe, sustainable, and abundant natural antioxidant sources. Here we invest... more Plant by-products are safe, sustainable, and abundant natural antioxidant sources. Here we investigated the antioxidant activity of a mixture of lyophilized pomegranate, guava, and grape (PGG) leaves water extract (1:1:1) and examined its ability to retard the rancidity of soybean oil during accelerated storage at 65 °C for 30 days. To achieve this, we evaluated the oxidative stability of soybean oil enriched with PGG extract at 200, 400, and 800 ppm. We also compared the effect of PGG extract with butylated hydroxytoluene (BHT) (400/100 ppm) with that of only BHT (200 ppm). We observed that 8.19 and 1.78 µg/mL of the extract could scavenge 50% of DPPH• and ABTS•, respectively, indicating its enhanced antioxidant activity. Enriching soyabean oil with the extract at 800 ppm improved its oxidative stability by reducing the acid value to 1.71 mg/g and the total oxidation to 99.87 compared to 2.27 mg/g and 150.32 in the raw oil, respectively. Moreover, PGG-800 ppm inhibited oxidation by...

Antioxidants, 2022

Obesity is one of the most prevalent non-communicable diseases and is interlinked with incidences... more Obesity is one of the most prevalent non-communicable diseases and is interlinked with incidences of various diseases. By modulating lifestyle and food quality, obesity can be preventable. The present study investigated the ability of a novel functional beverage based on orange juice and beetroot leaf and stem juice in preventing obesity-associated health issues. To achieve this purpose, we determined the nutritive value of juices and tested their ability to prevent the effect of a high-fat diet on a rat model. Adding leaf and stem juice to orange juice increased the total soluble solids/total titratable acidity ratio, which reflects the high acceptability of the blends, and enhanced their nutritive value. All minerals increased in the blends by increasing the leaf and stem juice percentage. Copper was detected only in the juices containing 10–20% leaf and stem juice (0.01–0.11 mg/100 g). Total flavonoids and betalain increased in the blends, reaching 142.02 µg/mL and 1680 µg/mL, re...

Journal of Functional Foods, 2021

Abstract The present study incorporated beetroot pomace powder in biscuit preparation at concentr... more Abstract The present study incorporated beetroot pomace powder in biscuit preparation at concentrations of 5–20%. Biscuits' chemical composition, and sensory were evaluated. A feeding experiment was conducted using three groups (n = 3); control and anemic (An+) groups fed a standard diet, and anemic group fed 90% standard-diet and 10% biscuit containing 15% pomace (AnB). Anemia induced by injecting rats with phenylhydrazine (40 mg/kg) for two consecutive days. Hematological and biochemical blood parameters were determined at days 3, 14, and 28. An enhancement in protein, fiber, calcium, phosphorus, and iron was observed in the biscuit by increasing the pomace. Consuming biscuits containing 15% pomace increased hemoglobin, RBCs, and antioxidant enzymes in (AnB) rats after 28 days. The minor kidney and liver dysfunction in the anemic groups were recovered in (AnB) after 28 days. Consequently, incorporation beet pomace in biscuit revealed a promising role in anemia treatment and oxidative stress managing.

Food and Nutrition Sciences, 2014

Bt corn "MON810: Ajeeb YG" produces delta endotoxins in the whole plant due to the genetic modifi... more Bt corn "MON810: Ajeeb YG" produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart "Ajeeb". Further, feeding studies on rats were designed to complete assessing the safety of "MON810: Ajeeb YG". Three groups of rats (6 males and 6 females' rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.

Plants, 2020

Beetroot is a good source of minerals, fibers, and bioactive components. The present research wor... more Beetroot is a good source of minerals, fibers, and bioactive components. The present research work was conducted to evaluate the nutritional quality of beetroots (juice, peels, leaves and pomace) enhancing the extracted bioactive components, and developing a functional probiotic beverage. Chemical composition and minerals content of beetroot parts were estimated. The bioactive components were extracted by instant extraction method (IEM) and overnight extraction method (at −20 °C) (OEM) to determine total phenolics, flavonoids, and DPPH inhibition ratio. The extracted beetroot juice was mixed with milk for valorization of the beverage nutritional value and fermented with LA-5 and ABT-5 cultures to create a novel functional beverage. Chemical composition, minerals content, and bioactive components of beverages were estimated. The leaves exhibited the highest calcium content (1200 mg/100 g). Juice showed the highest amount of all minerals except for calcium and magnesium. Overnight ext...