eman habib - Academia.edu (original) (raw)
Papers by eman habib
World Journal of Hepatology, Mar 27, 2021
BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. A... more BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. AIM To investigate the promotive action of 2-acetylaminofluorene (2-AAF) when combined with DEN in order to develop a rat model for induction of precancerous lesion and investigate the molecular mechanism underlying the activity of 2-AAF. METHODS The pre-precancerous lesions were initiated by intraperitoneal injection of DEN for three weeks consecutively, followed by one intraperitoneal injection of 2-AAF at three different doses (100, 200 and 300 mg/kg). Rats were separated into naïve, DEN, DEN + 100 mg 2-AAF, DEN + 200 mg 2-AAF, and DEN + 300 mg 2-AAF groups. Rats were sacrificed after 10 wk and 16 wk. Liver functions, level of alpha-fetoprotein, glutathione S-transferase-P and proliferating cell nuclear antigen staining of liver tissues were performed. The mRNA level of RAB11A, BAX, p53, and Cyclin E and epigenetic regulation by long-noncoding RNA (lncRNA) RP11-513I15.6, miR-1262 (microRNA), and miR-1298 were assessed in the sera and liver tissues of the rats. RESULTS 2-AAF administration significantly increased the percent area of the precancerous Hasanin AH et al. 2-AAF promoted hepatic precancerous lesions WJH https://www.wjgnet.com 329 March 27, 2021 Volume 13 Issue 3 additional data are available.
The Egyptian Journal of Anatomy (Print), Mar 30, 2019
Fundamental & Clinical Pharmacology, Mar 15, 2023
The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacolo... more The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacological agent. This study investigated the beneficial effects of thiorphan (TH)/irbesartan (IRB), in myocardial ischemia–reperfusion (IR) injury, compared to each of nitroglycerin and carvedilol. Male Wistar rats were divided into five groups (10 rats/group): Sham, untreated I/R, TH/IRB + IR (0.1/10 mg/kg), nitroglycerin + IR (0.2 mg/kg), and carvedilol + IR (10 mg/kg). Mean arterial blood pressure, cardiac functions and arrhythmia incidence, duration and score were assessed. Cardiac levels of creatine kinase‐MB (CK‐MB), oxidative stress, endothelin‐1, ATP, Na+/K+ ATPase pump activity and mitochondria complexes activities were measured. Histopathological examination, Bcl/Bax immunohistochemistry studies and electron microscopy examination of left ventricle were performed. TH/IRB preserved the cardiac functions and mitochondrial complexes activities, mitigated cardiac damage, reduced oxidative stress and arrhythmia severity, improved the histopathological changes and decreased cardiac apoptosis. TH/IRB showed a comparable effect to each of nitroglycerin and carvedilol in alleviating the IR injury consequences. TH/IRB showed significant preservation of mitochondrial complexes activity I and II compared to nitroglycerin. TH/IRB significantly increased LVdP/dtmax and decreased oxidative stress, cardiac damage and endothelin‐1 along with increasing the ATP content, Na+/K+ ATPase pump activity and mitochondrial complexes activity when compared to carvedilol. TH/IRB showed a cardioprotective effect in reducing IR injury that is comparable to each of nitroglycerin and carvedilol that could be explained in part by its ability to preserve mitochondrial function, increase ATP, decrease oxidative stress as well as endothelin 1.
Ain Shams medical journal, 2013
QJM: An International Journal of Medicine, Oct 1, 2021
drinking water) showed a significant decrease in the immobility time in the FST and TST and enhan... more drinking water) showed a significant decrease in the immobility time in the FST and TST and enhanced pain related behaviour in formalin test in the BCG-inoculated group. However, it did not affect the increase in the pain threshold in the planter incision allodynia model. Adding ibuprofen to drinking water (0.2 g/ L of drinking water), reversed the depressive like behaviour induced by BCG and enhanced pain-related behaviour in formalin test, in both the total pain-related behaviour and phase 2. It also prevented the increase in the base line induced by BCG. On the other hand, the incisional pain model was not affected by BCG inoculation except at the 2-hour time point, where it showed hypoalgesia, as well.
Fundamental & Clinical Pharmacology, Aug 2, 2022
Background: We aimed to determine whether the angiotensin receptor neprilysin inhibitor (SAC/VAL)... more Background: We aimed to determine whether the angiotensin receptor neprilysin inhibitor (SAC/VAL) promotes myocardial angiogenesis and improves perfusion vs valsartan (VAL) alone or controls. Methods: Rats (n=91) with left coronary artery ligation were randomized at 1-wk post to treatment (SAC/VAL, VAL, CTRL) for either 1 or 5 wk. Angiogenesis was assessed by an RGD peptide (99m Tc-NC100692) with 201 Tl perfusion evaluated at rest (1 wk post-tx) and under dobutamine stress (5 wk post-tx). Images were analyzed as the ratio of ischemic to normal area. Following imaging, animals were sacrificed, and the LV sectioned for well counting with corrected counts converted to % injected dose per gram of tissue (%ID/g). Infarct size, angiogenesis, and cardiomyocyte (CM) size histology was performed. Results: SAC/VAL therapy improved infarct 99m Tc-NC100692 uptake at 1 wk post-tx with no observed difference between groups at 5wk post-tx (Fig1A). At 5wk post-tx dobutamine-induced 201 Tl uptake significantly increased in SAC/VAL compared to CTRL (Fig 1B). SAC/VAL attenuated the increase in peri-infarct CM size at 5 wk post-tx vs CTRL (Fig1C) and both VAL and SAC/VAL prevented CM hypertrophy in remote regions vs CTRL. CM hypertrophy observed at 5 wk post-tx in peri-infarct region was accompanied by an increase in capillary density (Fig1D). Conclusion: SAC/VAL therapy improved angiogenesis with an augmentation in myocardial perfusion. This was observed with an increase in capillary density and attenuation of CM growth.
Biomedicine & Pharmacotherapy, 2019
The present study was conducted to investigate the potential protective effects of coenzyme Q 10 ... more The present study was conducted to investigate the potential protective effects of coenzyme Q 10 (CoQ10) administration on methotrexate induced lung and liver fibrosis in rat model, and to explore our hypothesis regarding its possible mechanism of action through reactivation of autophagy pathway. Methotrexate induced fibrosis was achieved by intraperitoneal injections twice a week for 4 weeks. A combined treatment of CoQ10 and methotrexate were used. Blood samples for biochemical analysis, lung and livers tissue for biochemical and histopathological analysis, were investigated. Concomitant treatment of CoQ10 & methotrexate caused improvement in histological picture of the lung and liver tissues, liver function and oxidative stress biomarkers, modulation of autophagy genes [mammalian target of rapamycin (m-TOR), Microtubule-associated proteins 1 A/ 1B light chain 3 (MAP1LC3B), and Sequestosome 1 ubiquitin-binding protein p62 (p62/SQSTM1)] with simultaneous reduction in High Mobility Group Protein B1 (HMGB1). Based on our results we postulated that CoQ10 up regulates autophagy pathway that could explain its protective properties against lung and liver fibrosis caused by methotrexate treatment in current study rat model.
Biomedicine & Pharmacotherapy
World Journal of Hepatology
BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. A... more BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. AIM To investigate the promotive action of 2-acetylaminofluorene (2-AAF) when combined with DEN in order to develop a rat model for induction of precancerous lesion and investigate the molecular mechanism underlying the activity of 2-AAF. METHODS The pre-precancerous lesions were initiated by intraperitoneal injection of DEN for three weeks consecutively, followed by one intraperitoneal injection of 2-AAF at three different doses (100, 200 and 300 mg/kg). Rats were separated into naïve, DEN, DEN + 100 mg 2-AAF, DEN + 200 mg 2-AAF, and DEN + 300 mg 2-AAF groups. Rats were sacrificed after 10 wk and 16 wk. Liver functions, level of alpha-fetoprotein, glutathione S-transferase-P and proliferating cell nuclear antigen staining of liver tissues were performed. The mRNA level of RAB11A, BAX, p53, and Cyclin E and epigenetic regulation by long-noncoding RNA (lncRNA) RP11-513I15.6, miR-1262 (microRNA), and miR-1298 were assessed in the sera and liver tissues of the rats. RESULTS 2-AAF administration significantly increased the percent area of the precancerous Hasanin AH et al. 2-AAF promoted hepatic precancerous lesions WJH https://www.wjgnet.com 329 March 27, 2021 Volume 13 Issue 3 additional data are available.
Fundamental & Clinical Pharmacology
The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacolo... more The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacological agent. This study investigated the beneficial effects of thiorphan (TH)/irbesartan (IRB), in myocardial ischemia–reperfusion (IR) injury, compared to each of nitroglycerin and carvedilol. Male Wistar rats were divided into five groups (10 rats/group): Sham, untreated I/R, TH/IRB + IR (0.1/10 mg/kg), nitroglycerin + IR (0.2 mg/kg), and carvedilol + IR (10 mg/kg). Mean arterial blood pressure, cardiac functions and arrhythmia incidence, duration and score were assessed. Cardiac levels of creatine kinase‐MB (CK‐MB), oxidative stress, endothelin‐1, ATP, Na+/K+ ATPase pump activity and mitochondria complexes activities were measured. Histopathological examination, Bcl/Bax immunohistochemistry studies and electron microscopy examination of left ventricle were performed. TH/IRB preserved the cardiac functions and mitochondrial complexes activities, mitigated cardiac damage, reduced oxidat...
World Journal of Gastroenterology, 2021
BACKGROUND Cyanidin-3-O-glucoside (cyan) exhibits antioxidant and anticancer properties. The cell... more BACKGROUND Cyanidin-3-O-glucoside (cyan) exhibits antioxidant and anticancer properties. The cell cycle proteins and antimitotic drugs might be promising therapeutic targets in hepatocellular carcinoma. AIM To investigate the effect of cyan administration on cell cycle in hepatic precancerous lesion (PCL) induced by diethylnitrosamine/2-acetylaminofluorene (DEN/2-AAF) in Wistar rats. METHODS In vivo, DEN/2-AAF-induced hepatic PCL, rats were treated with three doses of cyan (10, 15, and 20 mg/kg/d, for four consecutive days per week for 16 wk). Blood and liver tissue samples were collected for measurement of the followings; alpha fetoprotein (AFP) liver function and RNA panel differential expression was evaluated via real time polymerase chain reaction. Histopathological examination of liver sections stained with H&E and immunohistochemical study using glutathione S-transferase placental (GSTP) and proliferating cell nuclear antigen (PCNA) antibodies were assessed. RESULTS Cyan administration mitigated the effect of DEN/2-AFF induced PCL, decreased AFP levels, and improved liver function. Remarkably, treatment with cyan dose dependently decreased the long non-coding RNA MALAT1 and tubulin gamma 1 mRNA expressions and increased the levels of miR-125b, all of which are involved in cell cycle and mitotic spindle assembly. Of note, cyan decreased GSTP foci percent area and PCNA positively stained nuclei. CONCLUSION Our results indicated that cyan could be used as a potential therapeutic agent to inhibit liver carcinogenesis in rat model via modulation of cell cycle.
Egyptian Journal of Histology, 2021
QJM: An International Journal of Medicine, 2021
Background and Aim Diabetic neuropathy (DN) is the most common diabetic complication, which has a... more Background and Aim Diabetic neuropathy (DN) is the most common diabetic complication, which has a lifetime prevalence of about 50%. About 20 to 30% of patients with DN suffer from neuropathic pain. This study was designed to investigate the role of early and late treatment of pentoxifylline (PTX) in management of diabetic neuropathic pain (DNP). Method Diabetic rats were administered PTX (50 and 200 mg/kg/day, in drinking water) either one week after STZ injection and for 7 weeks or 6 weeks after STZ injection and for 2 weeks. Mechanical allodynia were assessed weekly, Iba-1 and GFAP immunorectivity, spinal NFκB & spinal sciatic TNF-α were estimated at the end of the study. Epidermal thickness of the food pad and Na+/K+-ATPase activity in sciatic nerve were measured as an outcome measure for predegenerative markers of DN. Results The results of the present study suggest that activated microglia and not astrocytes are involved in the development of experimental diabetic neuropathy. P...
Epigenomics, 2021
Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus ra... more Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes ( FYCO1, ULK, TECPR1 and WIPI2) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes. Conclusion: We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
Bulletin of Egyptian Society for Physiological Sciences, 2020
Background: Hepatic ischemia reperfusion (IR) injury is common with many medical strategies as li... more Background: Hepatic ischemia reperfusion (IR) injury is common with many medical strategies as liver transplantation and trauma. Aim: is comparing the efficacy and evaluating the possible mechanisms of ischemic preconditioning (IPC) and topical hypothermia (TH) in the hepatic IR injury. Method: 56 adult male albino rats were allocated into four groups: sham control group (n=15), hepatic ischemia reperfusion group (IR) (n=13):where ischemia compromised 70% of the liver for 45 min followed by 90 min of reperfusion, topical hypothermia (TH-IR) group (n=14): liver temperature was maintained during ischemia at 10°C, ischemic preconditioning group (IPC-IR) (n=14): 10 minutes of ischemia followed by 10 minutes of reperfusion prior to hepatic ischemia reperfusion. ALT, AST and ALP were measured in serum while malondialdehyde (MDA), glutathione peroxidase (GPX), hypoxia inducible 1 alpha (HIF1α), tumor necrosis factor alpha (TNFα), cytochrome c (Cyt c), adenosine triphosphate (ATP) and dynamine related protein (DRP1) were measured in hepatic tissue in addition to the histological examination. Results: TH and IPC significantly reduced AST, MDA, TNFα, Cyt c and DRP1 expression. TH preserved hepatic integrity, normalized both AST and ALT, increased hepatic GPX and reduced MDA while IPC did not increase GPX and has less significantly reduced MDA compared to hypothermia. ATP was significantly higher with hypothermia compared to IPC. Hypothermia decreased significantly the level of HIF1α while preconditioning increased its level. Conclusion: Hypothermia was more protective against IR injury compared to IPC despite reduced metabolic reprogramming signal HIF1α. Hypothermia effect was through improvement of REDOX state and mitochondrial bioenergetics. Keywords • Ischemia reperfusion injury • Hepatic hypothermia, • Hepatic ischemic preconditioning • Hypoxia inducible factor1 α (HIF1α) • ATP Bull. of Egyp. Soc. Physiol. Sci.
Current Genomics, 2020
Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in ... more Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control. Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA- 1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers. Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis. Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.
Neurochemistry International, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Journal of Cellular Biochemistry, 2019
The present study aimed to evaluate the potential therapeutic effect of pantoprazole, a proton‐pu... more The present study aimed to evaluate the potential therapeutic effect of pantoprazole, a proton‐pump inhibitor, on precancerous lesion (PCL) in rats. diethylnitrosamine and 2‐acetylaminofluorene were used to induce PCL in rats, in vivo. The rats were treated with three doses of pantoprazole (100, 50, and 25 mg/kg; three times weekly) during the last 4 weeks of the total 10 weeks of the experiment. Blood and liver tissue samples were collected for measurement of the exosomal abundance and exosomal competing endogenous RNA markers. Results revealed that pantoprazole administration had an ameliorating effect on liver function tests and microscopic features of the liver; and decreased exosome abundance in the liver tissue samples and sera of the rats. Meanwhile, the treatment also resulted in a dose‐dependent decrease in exosomal RAB11A mRNA and long noncoding RNA RP11‐513I15.6, which is an important participant in th exosomal secretion process with an increase in exosomal miRNA‐1262. B...
Gene, 2019
, Potential protective effects of Dapagliflozin in gentamicin induced nephrotoxicity rat model vi... more , Potential protective effects of Dapagliflozin in gentamicin induced nephrotoxicity rat model via modulation of apoptosis associated miRNAs, Gene,
QJM: An International Journal of Medicine, 2018
noted in all layers of the cortex in both intermediate and high doses treated groups. Conclusion:... more noted in all layers of the cortex in both intermediate and high doses treated groups. Conclusion: Sildenafil citrate caused hyperaemia and increased cerebellar blood flow in low dose and neurodegeneration with reactive astrocytosis within cerebellar cortex in high doses.
World Journal of Hepatology, Mar 27, 2021
BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. A... more BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. AIM To investigate the promotive action of 2-acetylaminofluorene (2-AAF) when combined with DEN in order to develop a rat model for induction of precancerous lesion and investigate the molecular mechanism underlying the activity of 2-AAF. METHODS The pre-precancerous lesions were initiated by intraperitoneal injection of DEN for three weeks consecutively, followed by one intraperitoneal injection of 2-AAF at three different doses (100, 200 and 300 mg/kg). Rats were separated into naïve, DEN, DEN + 100 mg 2-AAF, DEN + 200 mg 2-AAF, and DEN + 300 mg 2-AAF groups. Rats were sacrificed after 10 wk and 16 wk. Liver functions, level of alpha-fetoprotein, glutathione S-transferase-P and proliferating cell nuclear antigen staining of liver tissues were performed. The mRNA level of RAB11A, BAX, p53, and Cyclin E and epigenetic regulation by long-noncoding RNA (lncRNA) RP11-513I15.6, miR-1262 (microRNA), and miR-1298 were assessed in the sera and liver tissues of the rats. RESULTS 2-AAF administration significantly increased the percent area of the precancerous Hasanin AH et al. 2-AAF promoted hepatic precancerous lesions WJH https://www.wjgnet.com 329 March 27, 2021 Volume 13 Issue 3 additional data are available.
The Egyptian Journal of Anatomy (Print), Mar 30, 2019
Fundamental & Clinical Pharmacology, Mar 15, 2023
The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacolo... more The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacological agent. This study investigated the beneficial effects of thiorphan (TH)/irbesartan (IRB), in myocardial ischemia–reperfusion (IR) injury, compared to each of nitroglycerin and carvedilol. Male Wistar rats were divided into five groups (10 rats/group): Sham, untreated I/R, TH/IRB + IR (0.1/10 mg/kg), nitroglycerin + IR (0.2 mg/kg), and carvedilol + IR (10 mg/kg). Mean arterial blood pressure, cardiac functions and arrhythmia incidence, duration and score were assessed. Cardiac levels of creatine kinase‐MB (CK‐MB), oxidative stress, endothelin‐1, ATP, Na+/K+ ATPase pump activity and mitochondria complexes activities were measured. Histopathological examination, Bcl/Bax immunohistochemistry studies and electron microscopy examination of left ventricle were performed. TH/IRB preserved the cardiac functions and mitochondrial complexes activities, mitigated cardiac damage, reduced oxidative stress and arrhythmia severity, improved the histopathological changes and decreased cardiac apoptosis. TH/IRB showed a comparable effect to each of nitroglycerin and carvedilol in alleviating the IR injury consequences. TH/IRB showed significant preservation of mitochondrial complexes activity I and II compared to nitroglycerin. TH/IRB significantly increased LVdP/dtmax and decreased oxidative stress, cardiac damage and endothelin‐1 along with increasing the ATP content, Na+/K+ ATPase pump activity and mitochondrial complexes activity when compared to carvedilol. TH/IRB showed a cardioprotective effect in reducing IR injury that is comparable to each of nitroglycerin and carvedilol that could be explained in part by its ability to preserve mitochondrial function, increase ATP, decrease oxidative stress as well as endothelin 1.
Ain Shams medical journal, 2013
QJM: An International Journal of Medicine, Oct 1, 2021
drinking water) showed a significant decrease in the immobility time in the FST and TST and enhan... more drinking water) showed a significant decrease in the immobility time in the FST and TST and enhanced pain related behaviour in formalin test in the BCG-inoculated group. However, it did not affect the increase in the pain threshold in the planter incision allodynia model. Adding ibuprofen to drinking water (0.2 g/ L of drinking water), reversed the depressive like behaviour induced by BCG and enhanced pain-related behaviour in formalin test, in both the total pain-related behaviour and phase 2. It also prevented the increase in the base line induced by BCG. On the other hand, the incisional pain model was not affected by BCG inoculation except at the 2-hour time point, where it showed hypoalgesia, as well.
Fundamental & Clinical Pharmacology, Aug 2, 2022
Background: We aimed to determine whether the angiotensin receptor neprilysin inhibitor (SAC/VAL)... more Background: We aimed to determine whether the angiotensin receptor neprilysin inhibitor (SAC/VAL) promotes myocardial angiogenesis and improves perfusion vs valsartan (VAL) alone or controls. Methods: Rats (n=91) with left coronary artery ligation were randomized at 1-wk post to treatment (SAC/VAL, VAL, CTRL) for either 1 or 5 wk. Angiogenesis was assessed by an RGD peptide (99m Tc-NC100692) with 201 Tl perfusion evaluated at rest (1 wk post-tx) and under dobutamine stress (5 wk post-tx). Images were analyzed as the ratio of ischemic to normal area. Following imaging, animals were sacrificed, and the LV sectioned for well counting with corrected counts converted to % injected dose per gram of tissue (%ID/g). Infarct size, angiogenesis, and cardiomyocyte (CM) size histology was performed. Results: SAC/VAL therapy improved infarct 99m Tc-NC100692 uptake at 1 wk post-tx with no observed difference between groups at 5wk post-tx (Fig1A). At 5wk post-tx dobutamine-induced 201 Tl uptake significantly increased in SAC/VAL compared to CTRL (Fig 1B). SAC/VAL attenuated the increase in peri-infarct CM size at 5 wk post-tx vs CTRL (Fig1C) and both VAL and SAC/VAL prevented CM hypertrophy in remote regions vs CTRL. CM hypertrophy observed at 5 wk post-tx in peri-infarct region was accompanied by an increase in capillary density (Fig1D). Conclusion: SAC/VAL therapy improved angiogenesis with an augmentation in myocardial perfusion. This was observed with an increase in capillary density and attenuation of CM growth.
Biomedicine & Pharmacotherapy, 2019
The present study was conducted to investigate the potential protective effects of coenzyme Q 10 ... more The present study was conducted to investigate the potential protective effects of coenzyme Q 10 (CoQ10) administration on methotrexate induced lung and liver fibrosis in rat model, and to explore our hypothesis regarding its possible mechanism of action through reactivation of autophagy pathway. Methotrexate induced fibrosis was achieved by intraperitoneal injections twice a week for 4 weeks. A combined treatment of CoQ10 and methotrexate were used. Blood samples for biochemical analysis, lung and livers tissue for biochemical and histopathological analysis, were investigated. Concomitant treatment of CoQ10 & methotrexate caused improvement in histological picture of the lung and liver tissues, liver function and oxidative stress biomarkers, modulation of autophagy genes [mammalian target of rapamycin (m-TOR), Microtubule-associated proteins 1 A/ 1B light chain 3 (MAP1LC3B), and Sequestosome 1 ubiquitin-binding protein p62 (p62/SQSTM1)] with simultaneous reduction in High Mobility Group Protein B1 (HMGB1). Based on our results we postulated that CoQ10 up regulates autophagy pathway that could explain its protective properties against lung and liver fibrosis caused by methotrexate treatment in current study rat model.
Biomedicine & Pharmacotherapy
World Journal of Hepatology
BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. A... more BACKGROUND Diethylnitrosamine (DEN) induces hepatic neoplastic lesions over a prolonged period. AIM To investigate the promotive action of 2-acetylaminofluorene (2-AAF) when combined with DEN in order to develop a rat model for induction of precancerous lesion and investigate the molecular mechanism underlying the activity of 2-AAF. METHODS The pre-precancerous lesions were initiated by intraperitoneal injection of DEN for three weeks consecutively, followed by one intraperitoneal injection of 2-AAF at three different doses (100, 200 and 300 mg/kg). Rats were separated into naïve, DEN, DEN + 100 mg 2-AAF, DEN + 200 mg 2-AAF, and DEN + 300 mg 2-AAF groups. Rats were sacrificed after 10 wk and 16 wk. Liver functions, level of alpha-fetoprotein, glutathione S-transferase-P and proliferating cell nuclear antigen staining of liver tissues were performed. The mRNA level of RAB11A, BAX, p53, and Cyclin E and epigenetic regulation by long-noncoding RNA (lncRNA) RP11-513I15.6, miR-1262 (microRNA), and miR-1298 were assessed in the sera and liver tissues of the rats. RESULTS 2-AAF administration significantly increased the percent area of the precancerous Hasanin AH et al. 2-AAF promoted hepatic precancerous lesions WJH https://www.wjgnet.com 329 March 27, 2021 Volume 13 Issue 3 additional data are available.
Fundamental & Clinical Pharmacology
The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacolo... more The combined angiotensin receptor neprilysin inhibitor is a promising cardioprotective pharmacological agent. This study investigated the beneficial effects of thiorphan (TH)/irbesartan (IRB), in myocardial ischemia–reperfusion (IR) injury, compared to each of nitroglycerin and carvedilol. Male Wistar rats were divided into five groups (10 rats/group): Sham, untreated I/R, TH/IRB + IR (0.1/10 mg/kg), nitroglycerin + IR (0.2 mg/kg), and carvedilol + IR (10 mg/kg). Mean arterial blood pressure, cardiac functions and arrhythmia incidence, duration and score were assessed. Cardiac levels of creatine kinase‐MB (CK‐MB), oxidative stress, endothelin‐1, ATP, Na+/K+ ATPase pump activity and mitochondria complexes activities were measured. Histopathological examination, Bcl/Bax immunohistochemistry studies and electron microscopy examination of left ventricle were performed. TH/IRB preserved the cardiac functions and mitochondrial complexes activities, mitigated cardiac damage, reduced oxidat...
World Journal of Gastroenterology, 2021
BACKGROUND Cyanidin-3-O-glucoside (cyan) exhibits antioxidant and anticancer properties. The cell... more BACKGROUND Cyanidin-3-O-glucoside (cyan) exhibits antioxidant and anticancer properties. The cell cycle proteins and antimitotic drugs might be promising therapeutic targets in hepatocellular carcinoma. AIM To investigate the effect of cyan administration on cell cycle in hepatic precancerous lesion (PCL) induced by diethylnitrosamine/2-acetylaminofluorene (DEN/2-AAF) in Wistar rats. METHODS In vivo, DEN/2-AAF-induced hepatic PCL, rats were treated with three doses of cyan (10, 15, and 20 mg/kg/d, for four consecutive days per week for 16 wk). Blood and liver tissue samples were collected for measurement of the followings; alpha fetoprotein (AFP) liver function and RNA panel differential expression was evaluated via real time polymerase chain reaction. Histopathological examination of liver sections stained with H&E and immunohistochemical study using glutathione S-transferase placental (GSTP) and proliferating cell nuclear antigen (PCNA) antibodies were assessed. RESULTS Cyan administration mitigated the effect of DEN/2-AFF induced PCL, decreased AFP levels, and improved liver function. Remarkably, treatment with cyan dose dependently decreased the long non-coding RNA MALAT1 and tubulin gamma 1 mRNA expressions and increased the levels of miR-125b, all of which are involved in cell cycle and mitotic spindle assembly. Of note, cyan decreased GSTP foci percent area and PCNA positively stained nuclei. CONCLUSION Our results indicated that cyan could be used as a potential therapeutic agent to inhibit liver carcinogenesis in rat model via modulation of cell cycle.
Egyptian Journal of Histology, 2021
QJM: An International Journal of Medicine, 2021
Background and Aim Diabetic neuropathy (DN) is the most common diabetic complication, which has a... more Background and Aim Diabetic neuropathy (DN) is the most common diabetic complication, which has a lifetime prevalence of about 50%. About 20 to 30% of patients with DN suffer from neuropathic pain. This study was designed to investigate the role of early and late treatment of pentoxifylline (PTX) in management of diabetic neuropathic pain (DNP). Method Diabetic rats were administered PTX (50 and 200 mg/kg/day, in drinking water) either one week after STZ injection and for 7 weeks or 6 weeks after STZ injection and for 2 weeks. Mechanical allodynia were assessed weekly, Iba-1 and GFAP immunorectivity, spinal NFκB & spinal sciatic TNF-α were estimated at the end of the study. Epidermal thickness of the food pad and Na+/K+-ATPase activity in sciatic nerve were measured as an outcome measure for predegenerative markers of DN. Results The results of the present study suggest that activated microglia and not astrocytes are involved in the development of experimental diabetic neuropathy. P...
Epigenomics, 2021
Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus ra... more Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes ( FYCO1, ULK, TECPR1 and WIPI2) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes. Conclusion: We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
Bulletin of Egyptian Society for Physiological Sciences, 2020
Background: Hepatic ischemia reperfusion (IR) injury is common with many medical strategies as li... more Background: Hepatic ischemia reperfusion (IR) injury is common with many medical strategies as liver transplantation and trauma. Aim: is comparing the efficacy and evaluating the possible mechanisms of ischemic preconditioning (IPC) and topical hypothermia (TH) in the hepatic IR injury. Method: 56 adult male albino rats were allocated into four groups: sham control group (n=15), hepatic ischemia reperfusion group (IR) (n=13):where ischemia compromised 70% of the liver for 45 min followed by 90 min of reperfusion, topical hypothermia (TH-IR) group (n=14): liver temperature was maintained during ischemia at 10°C, ischemic preconditioning group (IPC-IR) (n=14): 10 minutes of ischemia followed by 10 minutes of reperfusion prior to hepatic ischemia reperfusion. ALT, AST and ALP were measured in serum while malondialdehyde (MDA), glutathione peroxidase (GPX), hypoxia inducible 1 alpha (HIF1α), tumor necrosis factor alpha (TNFα), cytochrome c (Cyt c), adenosine triphosphate (ATP) and dynamine related protein (DRP1) were measured in hepatic tissue in addition to the histological examination. Results: TH and IPC significantly reduced AST, MDA, TNFα, Cyt c and DRP1 expression. TH preserved hepatic integrity, normalized both AST and ALT, increased hepatic GPX and reduced MDA while IPC did not increase GPX and has less significantly reduced MDA compared to hypothermia. ATP was significantly higher with hypothermia compared to IPC. Hypothermia decreased significantly the level of HIF1α while preconditioning increased its level. Conclusion: Hypothermia was more protective against IR injury compared to IPC despite reduced metabolic reprogramming signal HIF1α. Hypothermia effect was through improvement of REDOX state and mitochondrial bioenergetics. Keywords • Ischemia reperfusion injury • Hepatic hypothermia, • Hepatic ischemic preconditioning • Hypoxia inducible factor1 α (HIF1α) • ATP Bull. of Egyp. Soc. Physiol. Sci.
Current Genomics, 2020
Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in ... more Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control. Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA- 1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers. Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis. Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.
Neurochemistry International, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Journal of Cellular Biochemistry, 2019
The present study aimed to evaluate the potential therapeutic effect of pantoprazole, a proton‐pu... more The present study aimed to evaluate the potential therapeutic effect of pantoprazole, a proton‐pump inhibitor, on precancerous lesion (PCL) in rats. diethylnitrosamine and 2‐acetylaminofluorene were used to induce PCL in rats, in vivo. The rats were treated with three doses of pantoprazole (100, 50, and 25 mg/kg; three times weekly) during the last 4 weeks of the total 10 weeks of the experiment. Blood and liver tissue samples were collected for measurement of the exosomal abundance and exosomal competing endogenous RNA markers. Results revealed that pantoprazole administration had an ameliorating effect on liver function tests and microscopic features of the liver; and decreased exosome abundance in the liver tissue samples and sera of the rats. Meanwhile, the treatment also resulted in a dose‐dependent decrease in exosomal RAB11A mRNA and long noncoding RNA RP11‐513I15.6, which is an important participant in th exosomal secretion process with an increase in exosomal miRNA‐1262. B...
Gene, 2019
, Potential protective effects of Dapagliflozin in gentamicin induced nephrotoxicity rat model vi... more , Potential protective effects of Dapagliflozin in gentamicin induced nephrotoxicity rat model via modulation of apoptosis associated miRNAs, Gene,
QJM: An International Journal of Medicine, 2018
noted in all layers of the cortex in both intermediate and high doses treated groups. Conclusion:... more noted in all layers of the cortex in both intermediate and high doses treated groups. Conclusion: Sildenafil citrate caused hyperaemia and increased cerebellar blood flow in low dose and neurodegeneration with reactive astrocytosis within cerebellar cortex in high doses.