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Papers by eri kriswanto

Research paper thumbnail of Combine Approach of Proximal Fibula Osteotomy (PFO) Followed by Intra-Articular Dextrose Prolotherapy in Severe Medial Knee Osteoarthritis

Journal of Pain Research

Background: Knee osteoarthritis (OA) is a chronic and progressive degenerative disease. It result... more Background: Knee osteoarthritis (OA) is a chronic and progressive degenerative disease. It resulted from mechanical and chemical disorders that damage the joint and the underlying bone. The management of knee OA is challenging due to poor self-regeneration of connective tissues. Surgical treatment with prolotherapy approaches was conducted to treat medial compartment knee OA. Aim: To know the injection frequency to reach a 50% improvement in VAS score and WOMAC index. Methods: Six patients who suffered from late-stage medial compartment knee OA underwent PFO followed by twelve sessions of intra-articular dextrose prolotherapy. The subjective pain score, visual analog scale (VAS), was assessed based on the patient subjectiveness before and after treatment. Patients marked the score from 0 to 10 cm to describe the current pain state. The functional index, the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index used to evaluate the Patient's clinical symptoms. It ranges from 0 to 96 points consisting of three main sections: pain (total 20 points), stiffness (total: eight points), and physical function disability (total 68 points). Higher scores indicate severe symptoms and function. Results: Four patients showed pain relief and functional improvement with more than 50% scores of VAS and WOMAC after the treatment. Two patients received more than twelve doses of intra-articular dextrose prolotherapy due to a lack of progress. Conclusion: This study provides clinical evidence for a new treatment strategy for advanced knee OA. This combined therapy improves the patient's daily activity function and postpones the need for total knee arthroplasty (TKA).

Research paper thumbnail of Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

BIOCELL, 2020

Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacte... more Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay, Alizarin RedS , and alkaline phosphatase staining, and real-time PCR. Notably, 300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs, indicating their osteogenic potential. Moreover, a significant enhancement of osteogenesis gene markers and proteins was observed. We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation. These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.

Research paper thumbnail of The effect of green tea extract to angiogenesis in bone regeneration

Eurasian Journal of Biosciences, 2020

Background: Polyphenols contained in tea have anti-angiogenesis effects. Epigallocatechin-3-galla... more Background: Polyphenols contained in tea have anti-angiogenesis effects. Epigallocatechin-3-gallate (EGCC) extracted from green tea is a strong inhibitor of neutrophil-mediated angiogenesis, both in vitro and in vivo. Some studies have shown that oral or IP injection of ECGC could be a strong angiogenesis inhibition. Purpose: This study aims to determine the effect of green tea extract on angiogenesis in bone fracture healing. Method: The study utilized the Randomized Control Group Post Test-Only Design research. In the first draft, the sample was divided into several groups, i.e., K1 as a control group, and P1, P2, P3, and P4 as treatment groups. For the first draft, the experimental animals were terminated on the 10th day. The termination was undertaken on the 14th day for the second draft. This study utilized the Anova test to analyze the data. Results: The recommended dose for administering the green tea extract was 20 mg (p=0.447), then there would be a significant reduction (p...

Research paper thumbnail of Effect of Green Tea Extract to Angiogenesis in Bone Healing Experimental Study

Background Non-union is one of a big problem in orthopaedic field.The development of new blood ve... more Background Non-union is one of a big problem in orthopaedic field.The development of new blood vessels (angiogenesis) plays an essential role in bone healing process. Non-union may occurs in patients who take some anti-angiogenic agents. Green tea has powerful anti-angiogenic properties and our aim was to evaluate these properties in experimental bone healing. Materials and Methods This is Randomized Control Group Post Test-Only Design . 70 mices with right femur fracture was randomly divided in to 2 experiment models ( model I and II), Each model consist of five group which is treated with pure water, 15 mg green tea extract, 20 mg green tea extract, 25 mg green tea extract, and 30 mg green tea extract per sonde. Model I, after 10 days, the cell presenting VEGF was evaluated by immunohistochemistry. Model II, after 14 days, the new micro vessels evaluated by HE staining. The data was analyzed using Anova one-way. Results 20 mg of green tea extract decreases diameter of the b...

Research paper thumbnail of Combine Approach of Proximal Fibula Osteotomy (PFO) Followed by Intra-Articular Dextrose Prolotherapy in Severe Medial Knee Osteoarthritis

Journal of Pain Research

Background: Knee osteoarthritis (OA) is a chronic and progressive degenerative disease. It result... more Background: Knee osteoarthritis (OA) is a chronic and progressive degenerative disease. It resulted from mechanical and chemical disorders that damage the joint and the underlying bone. The management of knee OA is challenging due to poor self-regeneration of connective tissues. Surgical treatment with prolotherapy approaches was conducted to treat medial compartment knee OA. Aim: To know the injection frequency to reach a 50% improvement in VAS score and WOMAC index. Methods: Six patients who suffered from late-stage medial compartment knee OA underwent PFO followed by twelve sessions of intra-articular dextrose prolotherapy. The subjective pain score, visual analog scale (VAS), was assessed based on the patient subjectiveness before and after treatment. Patients marked the score from 0 to 10 cm to describe the current pain state. The functional index, the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index used to evaluate the Patient's clinical symptoms. It ranges from 0 to 96 points consisting of three main sections: pain (total 20 points), stiffness (total: eight points), and physical function disability (total 68 points). Higher scores indicate severe symptoms and function. Results: Four patients showed pain relief and functional improvement with more than 50% scores of VAS and WOMAC after the treatment. Two patients received more than twelve doses of intra-articular dextrose prolotherapy due to a lack of progress. Conclusion: This study provides clinical evidence for a new treatment strategy for advanced knee OA. This combined therapy improves the patient's daily activity function and postpones the need for total knee arthroplasty (TKA).

Research paper thumbnail of Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

BIOCELL, 2020

Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacte... more Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay, Alizarin RedS , and alkaline phosphatase staining, and real-time PCR. Notably, 300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs, indicating their osteogenic potential. Moreover, a significant enhancement of osteogenesis gene markers and proteins was observed. We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation. These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.

Research paper thumbnail of The effect of green tea extract to angiogenesis in bone regeneration

Eurasian Journal of Biosciences, 2020

Background: Polyphenols contained in tea have anti-angiogenesis effects. Epigallocatechin-3-galla... more Background: Polyphenols contained in tea have anti-angiogenesis effects. Epigallocatechin-3-gallate (EGCC) extracted from green tea is a strong inhibitor of neutrophil-mediated angiogenesis, both in vitro and in vivo. Some studies have shown that oral or IP injection of ECGC could be a strong angiogenesis inhibition. Purpose: This study aims to determine the effect of green tea extract on angiogenesis in bone fracture healing. Method: The study utilized the Randomized Control Group Post Test-Only Design research. In the first draft, the sample was divided into several groups, i.e., K1 as a control group, and P1, P2, P3, and P4 as treatment groups. For the first draft, the experimental animals were terminated on the 10th day. The termination was undertaken on the 14th day for the second draft. This study utilized the Anova test to analyze the data. Results: The recommended dose for administering the green tea extract was 20 mg (p=0.447), then there would be a significant reduction (p...

Research paper thumbnail of Effect of Green Tea Extract to Angiogenesis in Bone Healing Experimental Study

Background Non-union is one of a big problem in orthopaedic field.The development of new blood ve... more Background Non-union is one of a big problem in orthopaedic field.The development of new blood vessels (angiogenesis) plays an essential role in bone healing process. Non-union may occurs in patients who take some anti-angiogenic agents. Green tea has powerful anti-angiogenic properties and our aim was to evaluate these properties in experimental bone healing. Materials and Methods This is Randomized Control Group Post Test-Only Design . 70 mices with right femur fracture was randomly divided in to 2 experiment models ( model I and II), Each model consist of five group which is treated with pure water, 15 mg green tea extract, 20 mg green tea extract, 25 mg green tea extract, and 30 mg green tea extract per sonde. Model I, after 10 days, the cell presenting VEGF was evaluated by immunohistochemistry. Model II, after 14 days, the new micro vessels evaluated by HE staining. The data was analyzed using Anova one-way. Results 20 mg of green tea extract decreases diameter of the b...