fenping shen - Academia.edu (original) (raw)
Papers by fenping shen
目的 研制膜表达热休克蛋白( HSP) 70瘤苗并研究其抗肿瘤治疗作用.方法以膜表达 HSP70的真核表达载体 pDisplay- mbHSP70转染肠癌细胞株 CT26.用 G418抗性筛选获... more 目的 研制膜表达热休克蛋白( HSP) 70瘤苗并研究其抗肿瘤治疗作用.方法以膜表达 HSP70的真核表达载体 pDisplay- mbHSP70转染肠癌细胞株 CT26.用 G418抗性筛选获得阳性克隆,扩增并制备瘤苗.观察瘤苗在体外对小鼠脾淋巴细胞的增殖刺激效应;在 BALB/c小鼠结直肠癌模型中观察瘤苗的抗肿瘤治疗作用;并检测实验鼠的自然杀伤细胞 (NK)和细胞毒性 T淋巴细胞 (CTL)活性.结果激光共聚焦显微镜和流式细胞术均证明,大量 HSP70表达并结合在肠癌瘤苗细胞膜表面.比较对照,胞浆表达 HSP70瘤苗显示出较高的鼠淋巴细胞增殖刺激效应,较强的瘤体生长抑制,并使荷瘤鼠的生存时间延长;而膜表达 HSP70瘤苗比胞浆表达者有着更高的淋巴增殖刺激效应,更强的瘤体生长抑制作用,并使荷瘤鼠的生存时间进一步延长.结论 HSP70表达并结合在细胞膜表面的肠癌细胞瘤苗对结直肠癌 CT26荷瘤小鼠有更强的治疗作用。
Zhejiang Medical Journal, 2004
Objective To construct the vector expressing membrane-bound heat shock protein 70 (HSP70) and to ... more Objective To construct the vector expressing membrane-bound heat shock protein 70 (HSP70) and to transfect it to melanoma cells. Methods The HSP70 cDNA was obtained by RT-PCR of mRNA isolated from human embryo renal cells in reverse transcription. The PCR primers, including Sac Ⅱ and Sal Ⅰrestriction endonuclease sites, were designed. pDisplay, with signal peptide and PDGFR transmembrane sequence lying respectively at the two flanks of the multiple cloning site, was used to construct plasmid expressing membrane-bound HSP70 (mbHSP70) in eukaryotes and the DNA was inserted into the polyclone endonuclease sites in pDisplay. The construct pDisplay-mbHSP70 was verified by sequencing and transduced into melanoma cells. Result DNA sequencing showed that the vector expressing mbHSP70 in eukaryotes was correctly constructed; Electrophoresis for RT-PCR products, Western blotting, laser confocal microscopy and flow cytometry indicated that the melanoma cells expressing the protein onto the cel...
目的 研制超抗原金黄色葡萄球菌肠毒素A(SEA)锚定的膜表达热休克蛋白(HSP)70的肠癌细胞瘤苗,研究其抗肿瘤治疗作用.方法 以先期实验制备的膜表达HSP70肠癌细胞株CT26.扩增后以本研究... more 目的 研制超抗原金黄色葡萄球菌肠毒素A(SEA)锚定的膜表达热休克蛋白(HSP)70的肠癌细胞瘤苗,研究其抗肿瘤治疗作用.方法 以先期实验制备的膜表达HSP70肠癌细胞株CT26.扩增后以本研究组早先研制的跨膜型SEA蛋白转染法锚定肠癌细胞,灭活并制备双重修饰的肠癌细胞瘤苗.观察瘤苗在体外对小鼠脾淋巴细胞的增殖刺激效应;在BALB/c小鼠结直肠癌移植模型中观察瘤苗的抗癌治疗作用:瘤体生长抑制和荷瘤鼠的生存时间;并同时检测实验鼠的自然杀伤细胞(NK)和细胞毒淋巴细胞(CTL)活性.结果 激光共聚焦显微镜和流式细胞术均证明瘤苗细胞的双重修饰:SEA锚定,HSP70表达并结合在瘤苗细胞膜表面.比较对照,SEA锚定或膜表达HSP70的单一修饰瘤苗均显示出较高的鼠淋巴细胞增殖刺激效应;较强的瘤体生长抑制,并使荷瘤鼠的生存时间延长.而SEA锚定的膜表达HSP70的双重修饰瘤苗比单一修饰瘤苗,有着更高的淋巴增殖刺激效应,更强的瘤体生长抑制作用,并使荷瘤鼠的生存时间进一步延长.结论 成功研制了超抗原SEA锚定的膜表达HSP70的肠癌细胞瘤苗,此双重修饰瘤苗对结直肠癌荷瘤小鼠有更强的治疗作用。
Fatigue & Fracture of Engineering Materials & Structures, 2015
paper presents a damage mechanics method applied successfully to assess fatigue life of notched s... more paper presents a damage mechanics method applied successfully to assess fatigue life of notched specimens with plastic deformation at the notch tip. A damage-coupled elasto-plastic constitutive model is employed in which nonlinear kinematic hardening is considered. The accumulated damage is described by a stress-based damage model and a plastic strain-based damage model, which depend on the cyclic stress and accumulated plastic strain, respectively. A three-dimensional finite element implementation of these models is developed to predict the crack initiation life of notched specimens. Two cases, a notched plate under tension-compression loadings and an SAE notched shaft under bending-torsion loadings including non-proportional loadings, are studied and the predicted results are compared with experimental data.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2006
To construct the recombinant plasmid of RCAS1, to express and purify its fusion protein GST-RCAS1... more To construct the recombinant plasmid of RCAS1, to express and purify its fusion protein GST-RCAS1, and to investigate its biological function. RCAS1 encoding gene was amplified by RT-PCR from total RNA extract of MCF-7 cells and was ligated with expression plasmid vector pGEX-2T by T4 DNA ligase after digested by the restricted endonucleases BamH I and EcoR I. Then the ligated products were inserted into competence JM109 E. Coli and the positive recombinants were identified by restriction endonuclease digestion assay and DNA sequencing. The GST-RCAS1 fusion protein expression was induced by IPTG in BL21 E. Coli and was purified with GST column and identified by SDS-PAGE and Western blotting with anti-GST monoclonal antibody, anti-RCAS1 (N-18) and anti-RCAS1 (C-20) polyclonal antibody. The apoptosis of activated T cells induced by GST-RCAS1 fusion protein was detected by flow cytometry with Annexin V and propidium iodide (PI) staining. A 642 bp product was cloned by RT-PCR and the re...
International journal of molecular sciences, 2008
A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weig... more A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2005
To study lysosomes involvement in the degradation of ricin A chain. A lysosome-targeted singal KF... more To study lysosomes involvement in the degradation of ricin A chain. A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain (rRTA) and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B. The cytotoxicity of recombinant proteins was measured by the MTT method. Recombinant RTA-KFERQ was 49.87%, 54.18% and 88.68% less cytotoxic than RTA itself on the three cell lines HEPG2, Hela and A549, respectively. Lysosomes can degrade, but not completely inactivate RTA in different cells, suggesting cells may have other degradation pathways for RTA.
Journal of Zhejiang University SCIENCE B, 2006
Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (... more Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods: The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.
Journal of International Medical Research, 2009
The case of a 62-year old man diagnosed with radiation-induced meningioma (RIM) after treatment f... more The case of a 62-year old man diagnosed with radiation-induced meningioma (RIM) after treatment for astrocytoma with an unusually short latency period of 7 months is reported. The patient first presented with a 2-month history of memory decline. Magnetic resonance imaging (MRI) showed a tumour in the left parieto-temporal lobe. Gross total resection was performed and the tumour was confirmed to be an astrocytoma. The patient received cranial radiotherapy 2 weeks later, however 7 months after radiation treatment the patient presented with headache and vomiting. MRI showed massive meningeal enhancement in the left frontal lobe, which progressively enlarged. The patient's clinical condition deteriorated and a second craniotomy was performed with complete removal of the secondary tumour, which was shown to be a malignant meningioma. Immunohistochemical staining identified CD133-positive cells in both tumours. A rare fraction of brain tumour stem cells (BTSC) was isolated from the pr...
Journal of Biomaterials Science, Polymer Edition, 2007
Polyethylenimine (PEI) is the polymer most commonly used for transferring plasmids into eukaryote... more Polyethylenimine (PEI) is the polymer most commonly used for transferring plasmids into eukaryotes, but its gene-transfer efficiency is lower compared to viral vectors. Receptors targeting PEI combined with ligands can enhance efficiency of gene transfer into the corresponding receptor-positive cells. Using the double-receptor-mediated pathway of viral infection, in this study we synthesized a novel non-viral vector based on PEI combined with two peptides recognizing FGF receptors (peptide YC25) and integrins (peptide CP9) on the cell surface. The dual targeting vector showed a physicochemical character similar to that of PEI, such as pDNA formation, particle size, zeta potential and lower toxicity. In vitro gene transfer showed that the dual-receptor targeted vector (YC25-PEI-CP9) exhibited a markedly higher transgene efficiency in cell lines with positive expression of FGF receptors and integrins, compared with single-peptide-modified PEI or unmodified PEI. In the cells with only integrin-positive expression, YC25-PEI-CP9 mediated a higher transgene expression than PEI but lower than CP9-PEI. The corresponding free peptides could inhibit the transgene efficiency of the peptide-coupled PEI. In vivo gene transfer in tumor-bearing nude mice also demonstrated that the dual-targeting vectors showed a significantly enhanced transfection efficiency in tumors with positive expression of FGF receptors and integrins. The synthesized polymer YC25-PEI-CP9 has the prospect to act as a novel kind of non-viral vector in gene therapy.
Journal of Biomaterials Applications, 2006
To obtain new nonviral vectors with high gene delivery efficiency and special cell targeting abil... more To obtain new nonviral vectors with high gene delivery efficiency and special cell targeting ability, an attractive strategy is to link ligands to polyethylenimine (PEI). Fibroblast growth factor receptors (FGFRs) are highly expressed on a variety of human cancer cells and are potential targets for cancer gene therapy. In this study, the peptides NH2-Met-Gln-Leu-Pro-Leu-Ala-ThrGly-Gly-Gly-Cys-COOH (MC11) which have been proved to combine specially with the FGFR on cell membrane are coupled to PEI using N-Succinimidyl-3-(2-pyridyldithio) propionate (SPDP) as a linker with different molar ratios (1 : 0.3, 1 : 0.75, 1 : 1.5, and 1 : 3.0) and the new polymer PEI-MC11 is verified by a series of physicochemical methods including 1H-NMR and FTIR. The agarose gel electrophoresis assay, particle size test, zeta potential test, and electron microscope observation show that PEI-MC11 can efficiently condense plasmid DNA into nanoparticles with about 200 nm in diameter and with positive surface ...
Chem. Commun., 2006
Two novel polymers of low molecular weight polyethylenimine cross-linked by (2-hydroxypropyl)-bet... more Two novel polymers of low molecular weight polyethylenimine cross-linked by (2-hydroxypropyl)-beta-cyclodextrin or (2-hydroxypropyl)-gamma-cyclodextrin showed lower cytotoxicity and higher transfection efficiency for the delivery of plasmid DNA compared with those of polyethylenimine (PEI, 25 kDa).
目的 研制膜表达热休克蛋白( HSP) 70瘤苗并研究其抗肿瘤治疗作用.方法以膜表达 HSP70的真核表达载体 pDisplay- mbHSP70转染肠癌细胞株 CT26.用 G418抗性筛选获... more 目的 研制膜表达热休克蛋白( HSP) 70瘤苗并研究其抗肿瘤治疗作用.方法以膜表达 HSP70的真核表达载体 pDisplay- mbHSP70转染肠癌细胞株 CT26.用 G418抗性筛选获得阳性克隆,扩增并制备瘤苗.观察瘤苗在体外对小鼠脾淋巴细胞的增殖刺激效应;在 BALB/c小鼠结直肠癌模型中观察瘤苗的抗肿瘤治疗作用;并检测实验鼠的自然杀伤细胞 (NK)和细胞毒性 T淋巴细胞 (CTL)活性.结果激光共聚焦显微镜和流式细胞术均证明,大量 HSP70表达并结合在肠癌瘤苗细胞膜表面.比较对照,胞浆表达 HSP70瘤苗显示出较高的鼠淋巴细胞增殖刺激效应,较强的瘤体生长抑制,并使荷瘤鼠的生存时间延长;而膜表达 HSP70瘤苗比胞浆表达者有着更高的淋巴增殖刺激效应,更强的瘤体生长抑制作用,并使荷瘤鼠的生存时间进一步延长.结论 HSP70表达并结合在细胞膜表面的肠癌细胞瘤苗对结直肠癌 CT26荷瘤小鼠有更强的治疗作用。
Zhejiang Medical Journal, 2004
Objective To construct the vector expressing membrane-bound heat shock protein 70 (HSP70) and to ... more Objective To construct the vector expressing membrane-bound heat shock protein 70 (HSP70) and to transfect it to melanoma cells. Methods The HSP70 cDNA was obtained by RT-PCR of mRNA isolated from human embryo renal cells in reverse transcription. The PCR primers, including Sac Ⅱ and Sal Ⅰrestriction endonuclease sites, were designed. pDisplay, with signal peptide and PDGFR transmembrane sequence lying respectively at the two flanks of the multiple cloning site, was used to construct plasmid expressing membrane-bound HSP70 (mbHSP70) in eukaryotes and the DNA was inserted into the polyclone endonuclease sites in pDisplay. The construct pDisplay-mbHSP70 was verified by sequencing and transduced into melanoma cells. Result DNA sequencing showed that the vector expressing mbHSP70 in eukaryotes was correctly constructed; Electrophoresis for RT-PCR products, Western blotting, laser confocal microscopy and flow cytometry indicated that the melanoma cells expressing the protein onto the cel...
目的 研制超抗原金黄色葡萄球菌肠毒素A(SEA)锚定的膜表达热休克蛋白(HSP)70的肠癌细胞瘤苗,研究其抗肿瘤治疗作用.方法 以先期实验制备的膜表达HSP70肠癌细胞株CT26.扩增后以本研究... more 目的 研制超抗原金黄色葡萄球菌肠毒素A(SEA)锚定的膜表达热休克蛋白(HSP)70的肠癌细胞瘤苗,研究其抗肿瘤治疗作用.方法 以先期实验制备的膜表达HSP70肠癌细胞株CT26.扩增后以本研究组早先研制的跨膜型SEA蛋白转染法锚定肠癌细胞,灭活并制备双重修饰的肠癌细胞瘤苗.观察瘤苗在体外对小鼠脾淋巴细胞的增殖刺激效应;在BALB/c小鼠结直肠癌移植模型中观察瘤苗的抗癌治疗作用:瘤体生长抑制和荷瘤鼠的生存时间;并同时检测实验鼠的自然杀伤细胞(NK)和细胞毒淋巴细胞(CTL)活性.结果 激光共聚焦显微镜和流式细胞术均证明瘤苗细胞的双重修饰:SEA锚定,HSP70表达并结合在瘤苗细胞膜表面.比较对照,SEA锚定或膜表达HSP70的单一修饰瘤苗均显示出较高的鼠淋巴细胞增殖刺激效应;较强的瘤体生长抑制,并使荷瘤鼠的生存时间延长.而SEA锚定的膜表达HSP70的双重修饰瘤苗比单一修饰瘤苗,有着更高的淋巴增殖刺激效应,更强的瘤体生长抑制作用,并使荷瘤鼠的生存时间进一步延长.结论 成功研制了超抗原SEA锚定的膜表达HSP70的肠癌细胞瘤苗,此双重修饰瘤苗对结直肠癌荷瘤小鼠有更强的治疗作用。
Fatigue & Fracture of Engineering Materials & Structures, 2015
paper presents a damage mechanics method applied successfully to assess fatigue life of notched s... more paper presents a damage mechanics method applied successfully to assess fatigue life of notched specimens with plastic deformation at the notch tip. A damage-coupled elasto-plastic constitutive model is employed in which nonlinear kinematic hardening is considered. The accumulated damage is described by a stress-based damage model and a plastic strain-based damage model, which depend on the cyclic stress and accumulated plastic strain, respectively. A three-dimensional finite element implementation of these models is developed to predict the crack initiation life of notched specimens. Two cases, a notched plate under tension-compression loadings and an SAE notched shaft under bending-torsion loadings including non-proportional loadings, are studied and the predicted results are compared with experimental data.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2006
To construct the recombinant plasmid of RCAS1, to express and purify its fusion protein GST-RCAS1... more To construct the recombinant plasmid of RCAS1, to express and purify its fusion protein GST-RCAS1, and to investigate its biological function. RCAS1 encoding gene was amplified by RT-PCR from total RNA extract of MCF-7 cells and was ligated with expression plasmid vector pGEX-2T by T4 DNA ligase after digested by the restricted endonucleases BamH I and EcoR I. Then the ligated products were inserted into competence JM109 E. Coli and the positive recombinants were identified by restriction endonuclease digestion assay and DNA sequencing. The GST-RCAS1 fusion protein expression was induced by IPTG in BL21 E. Coli and was purified with GST column and identified by SDS-PAGE and Western blotting with anti-GST monoclonal antibody, anti-RCAS1 (N-18) and anti-RCAS1 (C-20) polyclonal antibody. The apoptosis of activated T cells induced by GST-RCAS1 fusion protein was detected by flow cytometry with Annexin V and propidium iodide (PI) staining. A 642 bp product was cloned by RT-PCR and the re...
International journal of molecular sciences, 2008
A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weig... more A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2005
To study lysosomes involvement in the degradation of ricin A chain. A lysosome-targeted singal KF... more To study lysosomes involvement in the degradation of ricin A chain. A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain (rRTA) and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B. The cytotoxicity of recombinant proteins was measured by the MTT method. Recombinant RTA-KFERQ was 49.87%, 54.18% and 88.68% less cytotoxic than RTA itself on the three cell lines HEPG2, Hela and A549, respectively. Lysosomes can degrade, but not completely inactivate RTA in different cells, suggesting cells may have other degradation pathways for RTA.
Journal of Zhejiang University SCIENCE B, 2006
Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (... more Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods: The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.
Journal of International Medical Research, 2009
The case of a 62-year old man diagnosed with radiation-induced meningioma (RIM) after treatment f... more The case of a 62-year old man diagnosed with radiation-induced meningioma (RIM) after treatment for astrocytoma with an unusually short latency period of 7 months is reported. The patient first presented with a 2-month history of memory decline. Magnetic resonance imaging (MRI) showed a tumour in the left parieto-temporal lobe. Gross total resection was performed and the tumour was confirmed to be an astrocytoma. The patient received cranial radiotherapy 2 weeks later, however 7 months after radiation treatment the patient presented with headache and vomiting. MRI showed massive meningeal enhancement in the left frontal lobe, which progressively enlarged. The patient's clinical condition deteriorated and a second craniotomy was performed with complete removal of the secondary tumour, which was shown to be a malignant meningioma. Immunohistochemical staining identified CD133-positive cells in both tumours. A rare fraction of brain tumour stem cells (BTSC) was isolated from the pr...
Journal of Biomaterials Science, Polymer Edition, 2007
Polyethylenimine (PEI) is the polymer most commonly used for transferring plasmids into eukaryote... more Polyethylenimine (PEI) is the polymer most commonly used for transferring plasmids into eukaryotes, but its gene-transfer efficiency is lower compared to viral vectors. Receptors targeting PEI combined with ligands can enhance efficiency of gene transfer into the corresponding receptor-positive cells. Using the double-receptor-mediated pathway of viral infection, in this study we synthesized a novel non-viral vector based on PEI combined with two peptides recognizing FGF receptors (peptide YC25) and integrins (peptide CP9) on the cell surface. The dual targeting vector showed a physicochemical character similar to that of PEI, such as pDNA formation, particle size, zeta potential and lower toxicity. In vitro gene transfer showed that the dual-receptor targeted vector (YC25-PEI-CP9) exhibited a markedly higher transgene efficiency in cell lines with positive expression of FGF receptors and integrins, compared with single-peptide-modified PEI or unmodified PEI. In the cells with only integrin-positive expression, YC25-PEI-CP9 mediated a higher transgene expression than PEI but lower than CP9-PEI. The corresponding free peptides could inhibit the transgene efficiency of the peptide-coupled PEI. In vivo gene transfer in tumor-bearing nude mice also demonstrated that the dual-targeting vectors showed a significantly enhanced transfection efficiency in tumors with positive expression of FGF receptors and integrins. The synthesized polymer YC25-PEI-CP9 has the prospect to act as a novel kind of non-viral vector in gene therapy.
Journal of Biomaterials Applications, 2006
To obtain new nonviral vectors with high gene delivery efficiency and special cell targeting abil... more To obtain new nonviral vectors with high gene delivery efficiency and special cell targeting ability, an attractive strategy is to link ligands to polyethylenimine (PEI). Fibroblast growth factor receptors (FGFRs) are highly expressed on a variety of human cancer cells and are potential targets for cancer gene therapy. In this study, the peptides NH2-Met-Gln-Leu-Pro-Leu-Ala-ThrGly-Gly-Gly-Cys-COOH (MC11) which have been proved to combine specially with the FGFR on cell membrane are coupled to PEI using N-Succinimidyl-3-(2-pyridyldithio) propionate (SPDP) as a linker with different molar ratios (1 : 0.3, 1 : 0.75, 1 : 1.5, and 1 : 3.0) and the new polymer PEI-MC11 is verified by a series of physicochemical methods including 1H-NMR and FTIR. The agarose gel electrophoresis assay, particle size test, zeta potential test, and electron microscope observation show that PEI-MC11 can efficiently condense plasmid DNA into nanoparticles with about 200 nm in diameter and with positive surface ...
Chem. Commun., 2006
Two novel polymers of low molecular weight polyethylenimine cross-linked by (2-hydroxypropyl)-bet... more Two novel polymers of low molecular weight polyethylenimine cross-linked by (2-hydroxypropyl)-beta-cyclodextrin or (2-hydroxypropyl)-gamma-cyclodextrin showed lower cytotoxicity and higher transfection efficiency for the delivery of plasmid DNA compared with those of polyethylenimine (PEI, 25 kDa).