fernando ramos - Academia.edu (original) (raw)

Papers by fernando ramos

Haematologia, 1988

A 57 year old patient in whom Hodgkin's disease (HD) and Chronic Lymphocytic Leukemia (CLL) w... more A 57 year old patient in whom Hodgkin's disease (HD) and Chronic Lymphocytic Leukemia (CLL) was simultaneously diagnosed is described. The infiltration of peripheral blood and bone marrow by mature lymphocytes, with special immunological phenotype (SIg negative, mouse rosette positive and monoclonal antibodies B1+, B4+) consistent with B-CLL and histological findings of lymphnode and axillary mass biopsies were typical of HD. The immunological study of the cell suspension from the axillary mass displayed a phenotype similar to that of the peripheral blood lymphocytes. Whether HD and CLL are two processes of fortuitous association, or a single clinical entity remains to be elucidated. The immunological findings in our patient suggest a common origin for both disorders and that HD could sometimes be the result of a B cell proliferation.

Molecular and biochemical parasitology, 2005

The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an impo... more The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation and distribution of E. dispar strains obtained from cyst passers, in two neighbor rural communities in Morelos Mexico. We analyzed the polymorphic region of the quitinase protein gene in isolates of E. dispar. In 45 isolates from one community we identified 12 different CHI patterns while in 15 isolates from the other community we identified 5 different patterns. However both communities share 4 patterns. This finding suggests the presence of strains with different geographic mobility.

Experimental Parasitology, 2005

The objective of this work was to evaluate the frequency of Entamoeba histolytica/Entamoeba dispa... more The objective of this work was to evaluate the frequency of Entamoeba histolytica/Entamoeba dispar intestinal infection in HIV + / AIDS subjects and their HIV ¡ close relatives or sexual partners. Enteric parasites were investigated in stool samples by microscopic examination and E. histolytica and E. dispar were identiWed by PCR. We found by microscopic analysis in HIV + /AIDS group that the E. histolytica/E. dispar complex was present in 5.9% of the members, while in the HIV ¡ group was 2.9%. With PCR we found that the E. histolytica prevalence was 25.3% in the HIV + /AIDS group and 18.5% in the HIV-group. The diVerence in the results obtained with the microscopic and PCR is due to the diVerent sensibility of the procedures. Besides, we found patients who were infected with E. histolytica in both groups were asymptomatic cyst passers. Our results suggest that E. histolytica strains prevalent in the studied community appear to be of low pathogenic potential.

Experimental Parasitology, 2005

The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural com... more The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural community in the state of Morelos, Mexico, through PCR technique by using specie speciWc primer. The E. histolytica specie was detected in 33 of 290 analyzed stool samples (11.4%), E. dispar specie was observed in 21 samples (7.2%) and both species of Entamoeba were detected in seven samples (2.4%). So a higher E. histolytica than E. dispar frequency infection was detected (13.8 versus 9.6%). Even though in our design we did not considered the follow-up of included individuals, the absence of invasive amebiasis cases in the studied population during our stay in town was unexpected.

Archives of Medical Research, 2000

Background: Differential diagnosis of two protozoan parasites Entamoeba histolytica and E. dispar... more Background: Differential diagnosis of two protozoan parasites Entamoeba histolytica and E. dispar is of great clinical and epidemiological importance, but except in the case of haematophagous trophozoites in acute dysentery, it is not possible to differentiate them by microscopy. The present study was carried out from February 2005 to July 2006 in order to determine the prevalence of E. histolytica and E. dispar in Gonbad City, by using a PCR method. Methods: Five hundred and sixty four fecal samples were collected from primary health care centers of Gonbad both urban and rural areas. The stool specimens were examined by light microscopy (Direct slide smear, Iodine wet mount, Formalether concentration and Trichrome staining) to distinguish E. histolytica/E. dispar complex and differentiate them from other non-pathogenic intestinal amoebae. Results: The microscopy results of stool exams showed a frequency rate of 23 positive samples (4.07%) for cyst of E. histolytica/E. dispar complex. All the microscopy positive isolates appeared to be infected with cyst of E. histolytica/E. dispar complex were cultivated and maintained successfully in HSr + s medium for DNA extraction and identification by the PCR method. The PCR study showed that 16 isolates (69.56 %) of the Entamoeba samples were E. dispar while 7 samples (30.43%) of those microscopy positive samples were not amplified and none of them showed E. histolytica pattern. Conclusion: High frequency rate of E. dispar in this study were in high agreement with the estimation rate of these entamoebas worldwide.

Haematologia, 1988

A 57 year old patient in whom Hodgkin's disease (HD) and Chronic Lymphocytic Leukemia (CLL) w... more A 57 year old patient in whom Hodgkin's disease (HD) and Chronic Lymphocytic Leukemia (CLL) was simultaneously diagnosed is described. The infiltration of peripheral blood and bone marrow by mature lymphocytes, with special immunological phenotype (SIg negative, mouse rosette positive and monoclonal antibodies B1+, B4+) consistent with B-CLL and histological findings of lymphnode and axillary mass biopsies were typical of HD. The immunological study of the cell suspension from the axillary mass displayed a phenotype similar to that of the peripheral blood lymphocytes. Whether HD and CLL are two processes of fortuitous association, or a single clinical entity remains to be elucidated. The immunological findings in our patient suggest a common origin for both disorders and that HD could sometimes be the result of a B cell proliferation.

Molecular and biochemical parasitology, 2005

The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an impo... more The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation and distribution of E. dispar strains obtained from cyst passers, in two neighbor rural communities in Morelos Mexico. We analyzed the polymorphic region of the quitinase protein gene in isolates of E. dispar. In 45 isolates from one community we identified 12 different CHI patterns while in 15 isolates from the other community we identified 5 different patterns. However both communities share 4 patterns. This finding suggests the presence of strains with different geographic mobility.

Experimental Parasitology, 2005

The objective of this work was to evaluate the frequency of Entamoeba histolytica/Entamoeba dispa... more The objective of this work was to evaluate the frequency of Entamoeba histolytica/Entamoeba dispar intestinal infection in HIV + / AIDS subjects and their HIV ¡ close relatives or sexual partners. Enteric parasites were investigated in stool samples by microscopic examination and E. histolytica and E. dispar were identiWed by PCR. We found by microscopic analysis in HIV + /AIDS group that the E. histolytica/E. dispar complex was present in 5.9% of the members, while in the HIV ¡ group was 2.9%. With PCR we found that the E. histolytica prevalence was 25.3% in the HIV + /AIDS group and 18.5% in the HIV-group. The diVerence in the results obtained with the microscopic and PCR is due to the diVerent sensibility of the procedures. Besides, we found patients who were infected with E. histolytica in both groups were asymptomatic cyst passers. Our results suggest that E. histolytica strains prevalent in the studied community appear to be of low pathogenic potential.

Experimental Parasitology, 2005

The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural com... more The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural community in the state of Morelos, Mexico, through PCR technique by using specie speciWc primer. The E. histolytica specie was detected in 33 of 290 analyzed stool samples (11.4%), E. dispar specie was observed in 21 samples (7.2%) and both species of Entamoeba were detected in seven samples (2.4%). So a higher E. histolytica than E. dispar frequency infection was detected (13.8 versus 9.6%). Even though in our design we did not considered the follow-up of included individuals, the absence of invasive amebiasis cases in the studied population during our stay in town was unexpected.

Archives of Medical Research, 2000

Background: Differential diagnosis of two protozoan parasites Entamoeba histolytica and E. dispar... more Background: Differential diagnosis of two protozoan parasites Entamoeba histolytica and E. dispar is of great clinical and epidemiological importance, but except in the case of haematophagous trophozoites in acute dysentery, it is not possible to differentiate them by microscopy. The present study was carried out from February 2005 to July 2006 in order to determine the prevalence of E. histolytica and E. dispar in Gonbad City, by using a PCR method. Methods: Five hundred and sixty four fecal samples were collected from primary health care centers of Gonbad both urban and rural areas. The stool specimens were examined by light microscopy (Direct slide smear, Iodine wet mount, Formalether concentration and Trichrome staining) to distinguish E. histolytica/E. dispar complex and differentiate them from other non-pathogenic intestinal amoebae. Results: The microscopy results of stool exams showed a frequency rate of 23 positive samples (4.07%) for cyst of E. histolytica/E. dispar complex. All the microscopy positive isolates appeared to be infected with cyst of E. histolytica/E. dispar complex were cultivated and maintained successfully in HSr + s medium for DNA extraction and identification by the PCR method. The PCR study showed that 16 isolates (69.56 %) of the Entamoeba samples were E. dispar while 7 samples (30.43%) of those microscopy positive samples were not amplified and none of them showed E. histolytica pattern. Conclusion: High frequency rate of E. dispar in this study were in high agreement with the estimation rate of these entamoebas worldwide.