ghada agiz - Academia.edu (original) (raw)

Papers by ghada agiz

Food additives are substances internationally added to food, this may be natural or synthetic. Th... more Food additives are substances internationally added to food, this may be natural or synthetic. The safety of repeated exposure to permitted synthetic food additives (colorants or preservatives) has been questioned. Sodium nitrite (NaNO2, a food preservative agent) was used at 80 mg/kg/day and orally administered to the rats either alone or in conjugation with aqueous green tea extract (GTE, 10%) for 2 months. Blood and organs samples were collected for biochemical and histopathological examinations. Ingestion of NaNO2, induced a significant decline in blood glutathione reduced (GSH), plasma total antioxidant capacity (TAC) and glutathione-S-transferase (GST). Furthermore, significant increase was recorded in the level of serum lipid peroxide (MDA) and plasma nitric oxide (NO). Moreover, serum alanine transaminase (ALT), aspartate aminotransferase (AST) as well as bilirubin levels were increased significantly, while albumin level was decreased. Also, serum urea and creatinine levels were significantly increased. Plasma lactate dehydrogenase (LDH) and serum Creatine phosphokinase (CPK) activities were increased. Histopathological examination in sodium nitrite group rats revealed marked alterations in liver as apoptosis, periportal necrosis Sometimes, there were areas of focal necrosis which infiltrated with mononuclear cells giving the picture of granuloma like lesions, in addition to severe vacuolar degeneration. Also, kidney showed severe vacuolar degeneration and massive areas of tubular necrosis. Furthermore, heart showed necrosis of myocardial muscle cells. Fortunately, administration of green tea extract in conjugation with sodium nitrite showed significant amelioration of the investigated parameters and pathological changes. The results indicated that green tea extract has a potential to be developed as a preventive extract against sodium nitrite induced toxicity and the mechanism involved in the protection could be due to its antioxidant activity.

Ninety samples of raw, ultra heat temperature (UHT) and flavoured UHT milk (30 samples each) were... more Ninety samples of raw, ultra heat temperature (UHT) and flavoured UHT milk (30 samples each) were obtained from supermarkets in Kafr El- Sheikh, Egypt. The occurrence and concentration range of Aflatoxin M1 (AFM1) in the samples were investigated by competitive enzyme-linked immunoabsorbent assay (ELISA) method. AFM1 was found in 63 (70%) out of 90 milk samples examined. The levels of AFM1 in 16 (25.4%) samples were higher than the maximum tolerance limit (50 ng/l) accepted by some European countries while none of the samples exceeded the prescribed limit of US regulations (500 ng/l). The highest mean concentration of AFM1 was recorded in raw milk samples (55.7 ± 6.7ng/l). The lowest mean concentration of AFM1 was recorded in flavoured UHT milk samples (18.8± 4.8 ng/l). While, the mean concentration in UHT milk samples was 23.1± 4.7 ng/l. It was therefore concluded that, the levels of AFM1 in milk especially raw samples consumed in Kafr El-Sheikh, Egypt were high and seemed to pose ...

Fe, Zn, Cu, Pb and Cd concentrations were determined in muscle, gills and liver of fresh and salt... more Fe, Zn, Cu, Pb and Cd concentrations were determined in muscle, gills and liver of fresh and salted (either by analytical or industrial salt) mullet fish caught from El-Burullus Lake. Heavy metal concentrations varied significantly depending on the type of the tissue and salt. Generally, the lowest concentrations of the studied metals were found in fresh fish, while the highest concentrations were recorded in salted fish by industrial salt which were higher than salted fish by analytical one. Fish organs of fresh and salted fish by analytical salt contain metals followed the order: liver>gills>muscle, while in salted fish by industrial one showed: gills>muscle>liver. The metal levels were ranked as follow: Fe>Zn>Cu>Pb>Cd. Fe and Cd concentrations exceeded the upper limit of standards while, Zn one recorded much below level in all deliberate fish organs. Cu content was slightly higher than the permissible limit in muscle and gills of salted fish by industrial ...

Assiut veterinary medical journal, Jul 1, 2011