hadeeqa raza - Academia.edu (original) (raw)

Papers by hadeeqa raza

Overview of Cancer Genomics, Organization, and Variations in the Human Genome

'Essentials of Cancer Genomic, Computational Approaches and Precision Medicine, 2020

Clinical manifestations of complex diseases like cancer oblige deep knowledge of each attribute. ... more Clinical manifestations of complex diseases like cancer oblige deep knowledge of each attribute. Both structural and functional aspects of the genome are essential for the characterization of DNA intended for sequencing the whole genome, accompanied with the knowledge of bioinformatics analysis and computational simulation, improving the selection of target biomarkers and drug designing strategies. In this chapter, we have given a brief outline of cancer genomics, sequencing methods (conventional and next-generation), biomarkers, DNA, and tissue microarrays. Recent investigations in different types of cancer demonstrate its relationship with gene mutations which assign the specific chromosome and physical mapping of genome structure using both experimental and computational models. This chapter discusses the cost-effectiveness and labor intensity of techniques utilized for gene manipulations which advance the practices for biomedical and clinical diagnostics strategies. On the other hand, biomarkers are the biological molecules which act as indicators for certain diseases and can be elevated or reduced during certain pathological conditions, consequently revealing the route to the detection, diagnosis, prognosis, and predictions for many disease classifications. Mass-spectrometric analysis of whole protein sequences is also getting attention due to its promising approaches, facilitating diagnosis in the field of infections as well as in the indication of pathological stages, with the help of protein release profiling and expression both qualitatively and quantitatively. DNA microarrays and tissue microarrays are the techniques that have been evolved for the economization of cost and time for both DNA and tissue analysis, respectively, minimizing the laborious procedures for diagnosis. The advantages of these aforementioned techniques continue to progress, thus, mesmerizing the scientific world.

Identification and Characterization of Natural and Semisynthetic Quinones as Aurora Kinase Inhibitors

Journal of Natural Products

Overview of Cancer Genomics, Organization, and Variations in the Human Genome

'Essentials of Cancer Genomic, Computational Approaches and Precision Medicine

The FEBS Journal

Cyclic guanosine 3 0 ,5 0-monophosphate (cGMP) is an intracellular signalling molecule involved i... more Cyclic guanosine 3 0 ,5 0-monophosphate (cGMP) is an intracellular signalling molecule involved in many sensory and developmental processes. Synthesis of cGMP from GTP is catalysed by guanylate cyclase (GC) in a reaction analogous to cAMP formation by adenylate cyclase (AC). Although detailed structural information is available on the catalytic region of nucleotidyl cyclases (NCs) in various states, these atomic models do not provide a sufficient explanation for the substrate selectivity between GC and AC family members. Detailed structural information on the GC domain in its active conformation is largely missing, and no crystal structure of a GTP-bound wild-type GC domain has been published to date. Here, we describe the crystal structure of the catalytic domain of rhodopsin-GC (RhGC) from Catenaria anguillulae in complex with GTP at 1.7 A resolution. Our study reveals the organization of a eukaryotic GC domain in its active conformation. We observe that the binding mode of the substrate GTP is similar to that of AC-ATP interaction, although surprisingly not all of the interactions predicted to be responsible for base recognition are present. The structure provides insights into potential mechanisms of substrate discrimination and activity regulation that may be common to all class III purine NCs. Database Structural data are available in Protein Data Bank database under the accession number 6SIR. Enzymes EC 4.6.1.2. Abbreviations AC, adenylate cyclase; ATP, adenosine-5 0-triphosphate; cAMP, cyclic 3 0 ,5 0-adenosine monophosphate; cGMP, cyclic 3 0 ,5 0-guanosine monophosphate; GC, guanylate cyclase; GTP, guanosine-5 0-triphosphate; NC, nucleotidyl cyclase; sGC, soluble guanylate cyclase.

Overview of Cancer Genomics, Organization, and Variations in the Human Genome

'Essentials of Cancer Genomic, Computational Approaches and Precision Medicine, 2020

Clinical manifestations of complex diseases like cancer oblige deep knowledge of each attribute. ... more Clinical manifestations of complex diseases like cancer oblige deep knowledge of each attribute. Both structural and functional aspects of the genome are essential for the characterization of DNA intended for sequencing the whole genome, accompanied with the knowledge of bioinformatics analysis and computational simulation, improving the selection of target biomarkers and drug designing strategies. In this chapter, we have given a brief outline of cancer genomics, sequencing methods (conventional and next-generation), biomarkers, DNA, and tissue microarrays. Recent investigations in different types of cancer demonstrate its relationship with gene mutations which assign the specific chromosome and physical mapping of genome structure using both experimental and computational models. This chapter discusses the cost-effectiveness and labor intensity of techniques utilized for gene manipulations which advance the practices for biomedical and clinical diagnostics strategies. On the other hand, biomarkers are the biological molecules which act as indicators for certain diseases and can be elevated or reduced during certain pathological conditions, consequently revealing the route to the detection, diagnosis, prognosis, and predictions for many disease classifications. Mass-spectrometric analysis of whole protein sequences is also getting attention due to its promising approaches, facilitating diagnosis in the field of infections as well as in the indication of pathological stages, with the help of protein release profiling and expression both qualitatively and quantitatively. DNA microarrays and tissue microarrays are the techniques that have been evolved for the economization of cost and time for both DNA and tissue analysis, respectively, minimizing the laborious procedures for diagnosis. The advantages of these aforementioned techniques continue to progress, thus, mesmerizing the scientific world.

Identification and Characterization of Natural and Semisynthetic Quinones as Aurora Kinase Inhibitors

Journal of Natural Products

Overview of Cancer Genomics, Organization, and Variations in the Human Genome

'Essentials of Cancer Genomic, Computational Approaches and Precision Medicine

The FEBS Journal

Cyclic guanosine 3 0 ,5 0-monophosphate (cGMP) is an intracellular signalling molecule involved i... more Cyclic guanosine 3 0 ,5 0-monophosphate (cGMP) is an intracellular signalling molecule involved in many sensory and developmental processes. Synthesis of cGMP from GTP is catalysed by guanylate cyclase (GC) in a reaction analogous to cAMP formation by adenylate cyclase (AC). Although detailed structural information is available on the catalytic region of nucleotidyl cyclases (NCs) in various states, these atomic models do not provide a sufficient explanation for the substrate selectivity between GC and AC family members. Detailed structural information on the GC domain in its active conformation is largely missing, and no crystal structure of a GTP-bound wild-type GC domain has been published to date. Here, we describe the crystal structure of the catalytic domain of rhodopsin-GC (RhGC) from Catenaria anguillulae in complex with GTP at 1.7 A resolution. Our study reveals the organization of a eukaryotic GC domain in its active conformation. We observe that the binding mode of the substrate GTP is similar to that of AC-ATP interaction, although surprisingly not all of the interactions predicted to be responsible for base recognition are present. The structure provides insights into potential mechanisms of substrate discrimination and activity regulation that may be common to all class III purine NCs. Database Structural data are available in Protein Data Bank database under the accession number 6SIR. Enzymes EC 4.6.1.2. Abbreviations AC, adenylate cyclase; ATP, adenosine-5 0-triphosphate; cAMP, cyclic 3 0 ,5 0-adenosine monophosphate; cGMP, cyclic 3 0 ,5 0-guanosine monophosphate; GC, guanylate cyclase; GTP, guanosine-5 0-triphosphate; NC, nucleotidyl cyclase; sGC, soluble guanylate cyclase.