hakkı taştan - Academia.edu (original) (raw)
Papers by hakkı taştan
Journal of Oral Science, 2008
Localized aggressive periodontitis (LAgP) is a complex multifactorial periodontal disease to whic... more Localized aggressive periodontitis (LAgP) is a complex multifactorial periodontal disease to which genetic factors are thought to predispose individuals. Interleukin-1 (IL-1) and tumor necrosis factor-α (TNFα) are potent immunomodulators and proinflammatory cytokines that have been implicated in the pathogenesis of autoimmune and infectious diseases and proposed to be risk factors for LAgP. Our aim was to investigate I L-1 α + 4 8 4 5 , I L-1 β + 3 9 5 4 , a n d T N F-α-3 0 8 g e n e polymorphisms in Turkish LAgP patients. We genotyped 31 LAgP patients and 31 healthy controls for IL-1α +4845 , IL-1β +3954 , and TNF-α-308 using standard PCR amplification followed by restriction enzyme digestion and gel electrophoresis. Higher prevalence of heterozygosity for IL-1α +4845 was found in cases (65%) when compared to controls (35%) (P < 0.05). While homozygous allele 1 of IL-1β +3954 was the most frequent genotype in cases (62%), no controls were homozygous for this allele (P < 0.001). Homozygous allele 1 was the most common TNF-α genotype in both groups, however no significant difference in TNF-α genotypes was found between groups. In conclusion, in this Turkish population, susceptibility to LAgP is increased by heterozygosity for allele 1 of IL-1α +4845 or homozygosity for allele 1 of IL-1β +3954. Moreover, IL-1 gene polymorphisms appear to have a role in susceptibility to LAgP, and the above-mentioned genotypes could be an important risk factor for LAgP in the Turkish population.
Frontiers in Bioscience-Landmark
Taiwanese Journal of Obstetrics and Gynecology
Proceedings
The aim of the study was to investigate the effect of paclitaxel loaded nanocarrier systems (chit... more The aim of the study was to investigate the effect of paclitaxel loaded nanocarrier systems (chitosan nanoparticles or quantum dots) on breast cancer. Paclitaxel loaded chitosan nanoparticles and quantum dots were prepared with the particle size of 153.6 nm and 302.8 nm respectively. The activities nanocarrier systems were determined using 7-12-dimethylbenz a antracene (DMBA) induced breast cancer model in six-week-old female, nonpregnant, Wistar albino rats. Paclitaxel loaded nanocarrier systems were administered intraperitoneally to tumor bearing rats and their tumor volumes were measured. At the end of the experiment rats were sacrificed and their tissue sections were analyzed. Both nanocarrier systems (chitosan nanoparticles or quantum dots) successfully reduced the tumors size. This study provides some information about the preparation techniques of paclitaxel loaded nanocarriers and some possible alternatives or strategies for the treatment of breast cancer using these nanocarriers.
Journal of Ethnopharmacology
Journal of Ethnopharmacology
Saudi Pharmaceutical Journal
Open Chemistry
In Turkish folk medicine, the fruits of Pyrus elaeagnifolia subsp. elaeagnifolia have been used t... more In Turkish folk medicine, the fruits of Pyrus elaeagnifolia subsp. elaeagnifolia have been used to treat diarrhea and detoxify poisonous snake bites by enlarging the wound. The aim of the study was to confirm the ethnopharmacological usage of the plant using in vivo and in vitro models. Experimental colitis was performed under anesthesia by intrarectal administration of acetic acid in rats, and the extracts were administered orally. The colonic malondialdehyde (MDA), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nitrite levels, in addition to the myeloperoxidase (MPO) and caspase-3 activities, were measured to determine the effects of the plant extracts. The methanol (MeOH) extract revealed a significant decrease in MPO and caspase-3 levels. The MeOH extract was found to have the highest total tannin content. It was also found to have significant antioxidant (p ˂ 0.01) and anti-inflammatory activities (p ˂ 0.05) in acetic acid induced colitis rat model . According to our ...
Journal of Ethnopharmacology
Ethnobotanical investigations have shown that the Pinus species have been used against rheumatic ... more Ethnobotanical investigations have shown that the Pinus species have been used against rheumatic pain and for wound healing in Turkish folk medicine. In this study, phytochemical composition, antioxidant, anti-inflammatory, and wound healing activities of Maritime Pine (Pinus pinaster Ait.) that is collected in Turkey are investigated. Essential oil composition and the amount of extracts (lipophilic and hydrophilic) of maritime pine wood and fresh cone samples had been tested. The essential oil from cones of P. pinaster revealed the highest activities, whereas other parts of the plant did not display any appreciable wound healing, anti-inflammatory, or antioxidant effects. α-Pinene was the main constituent of the essential oil obtained from the cones of P. pinaster. Experimental studies shown that P. pinaster&amp;#39;s remarkable anti-inflammatory and wound healing activities support the traditional use of the plant, and suggest it could have a place in modern medicine.
Turkiye Klinikleri Tıp Bilimleri Dergisi, 1996
Turkiye Klinikleri Journal of Medical Sciences, 2004
Turkiye Klinikleri Journal of Medical Sciences, 2008
... ORIGINAL RESEARCH. Sitogenetik Araştırmalarda İnversiyon Analizi Hakkı TAŞTAN, MD a ,Ayla TÜZ... more ... ORIGINAL RESEARCH. Sitogenetik Araştırmalarda İnversiyon Analizi Hakkı TAŞTAN, MD a ,Ayla TÜZÜN, MD a ,Serdar YÜKSEL b a Department of Biology, Ankara University Faculty of Science b Department of Genetic, Dr. Zekai Tahir Burak Women's Hospital's, Ankara. ...
Turkiye Klinikleri Journal of Case Reports, 1998
Gene therapy & molecular biology
Objective: To assess the association between the MTHFR gene (Al298C), MTR gene (A2756G) and MTRR ... more Objective: To assess the association between the MTHFR gene (Al298C), MTR gene (A2756G) and MTRR gene (A66G) polymorphisms and nonsyndromic cleft lip with or without cleft palate(nsCL/P), we investigated patients in the Turkish population. Methodology: Analysis of gene polymorphisms was carried out using polymerase chain reactions and restriction enzyme digestions. Our study included 100 patients with nsCL/P and 125 age matched healty individuals. Results: We found that MTHFR Al298C polymorphism and MTR A2756G polymorphism are significant risk factors for nsCL/P. The frequency of MTRR A66G homozygotes was higher compared to control group, however the difference was not statistically significant. Conclusions: We revealed a statistical significant association between the MTHFR Al298C and MTR A2756G polymorphism and nsCL/P.
Archives of Oral Biology, 2014
Non-syndromic cleft lip with/without cleft palate (nsCL/P) has a complex aetiology involving both... more Non-syndromic cleft lip with/without cleft palate (nsCL/P) has a complex aetiology involving both genetic and environmental factors. The aim of this study was to investigate the association between PVRL1 gene mutations and Turkish patients with nsCL/P. In this study, 80 Turkish patients with nsCL/P and 125 unrealeted individuals were analyzed. Mutations were detected using polymerase chain reactions and DNA sequencing. We found a novel GGA insertion between nucleotide positions c.1311_1313delGGA in exon 6 of the PVRL1 gene. Fifteen of the 80 patients with nsCL/P had the GGA insertion, although no mutation was found in the 125 unrelated individuals. We identified new supportive evidence that the association between PVRL1 gene and nsCL/P.
International Journal of Pediatric Otorhinolaryngology, 2013
Orofacial clefts are common congenital defects, with a birth prevalence of approximately 1/1000 i... more Orofacial clefts are common congenital defects, with a birth prevalence of approximately 1/1000 in Caucasians [1,2]. Prevalence rate varies according to geographical origin [3], sex [4] racial background and ethnicity [5], socioeconomic status [6]. Orofacial clefts are classified based on the presence of other congenital anomalies: nonsyndromic cleft lip with or without cleft palate, nonsyndromic cleft palate only, syndromic cleft lip with or without cleft palate and syndromic cleft palate only about 70% of cleft lip with or without cleft palate cases are nonsyndromic form and syndromic cleft containing part of 30% can be seen together with more than 300 defined mendelian syndromes and uncategorized syndromes [7,8]. Nonsyndromic cleft lip with or without cleft palate (nsCL/P, MIM 119530) is one of the most common birth defects, involving genetic and environmental factors [9-11]. Environmental factors such as cigarette smoking and alcohol consumption, intake antiepileptic drugs, inadequate nutrition especially inadequate folate and B6 B12 intake during pregnancy [12-16]. To identify genetic factors, used linkage and association analyses. Several loci or candidate genes have been identified in nsCL/P by these analyses [11] at the linkage technique, chromosomes of family members with CL/P make comparison by looking at chromosomes of unaffected family members [17]. For association technique, investigations make on only cases with CL/P [7]. There are candidate genes that studied to investigate of CL/P genetically: MTHFR,
Turkiye Klinikleri Journal of Medical Sciences, 2011
The Photogrammetric Record, 2002
Journal of Oral Science, 2008
Localized aggressive periodontitis (LAgP) is a complex multifactorial periodontal disease to whic... more Localized aggressive periodontitis (LAgP) is a complex multifactorial periodontal disease to which genetic factors are thought to predispose individuals. Interleukin-1 (IL-1) and tumor necrosis factor-α (TNFα) are potent immunomodulators and proinflammatory cytokines that have been implicated in the pathogenesis of autoimmune and infectious diseases and proposed to be risk factors for LAgP. Our aim was to investigate I L-1 α + 4 8 4 5 , I L-1 β + 3 9 5 4 , a n d T N F-α-3 0 8 g e n e polymorphisms in Turkish LAgP patients. We genotyped 31 LAgP patients and 31 healthy controls for IL-1α +4845 , IL-1β +3954 , and TNF-α-308 using standard PCR amplification followed by restriction enzyme digestion and gel electrophoresis. Higher prevalence of heterozygosity for IL-1α +4845 was found in cases (65%) when compared to controls (35%) (P < 0.05). While homozygous allele 1 of IL-1β +3954 was the most frequent genotype in cases (62%), no controls were homozygous for this allele (P < 0.001). Homozygous allele 1 was the most common TNF-α genotype in both groups, however no significant difference in TNF-α genotypes was found between groups. In conclusion, in this Turkish population, susceptibility to LAgP is increased by heterozygosity for allele 1 of IL-1α +4845 or homozygosity for allele 1 of IL-1β +3954. Moreover, IL-1 gene polymorphisms appear to have a role in susceptibility to LAgP, and the above-mentioned genotypes could be an important risk factor for LAgP in the Turkish population.
Frontiers in Bioscience-Landmark
Taiwanese Journal of Obstetrics and Gynecology
Proceedings
The aim of the study was to investigate the effect of paclitaxel loaded nanocarrier systems (chit... more The aim of the study was to investigate the effect of paclitaxel loaded nanocarrier systems (chitosan nanoparticles or quantum dots) on breast cancer. Paclitaxel loaded chitosan nanoparticles and quantum dots were prepared with the particle size of 153.6 nm and 302.8 nm respectively. The activities nanocarrier systems were determined using 7-12-dimethylbenz a antracene (DMBA) induced breast cancer model in six-week-old female, nonpregnant, Wistar albino rats. Paclitaxel loaded nanocarrier systems were administered intraperitoneally to tumor bearing rats and their tumor volumes were measured. At the end of the experiment rats were sacrificed and their tissue sections were analyzed. Both nanocarrier systems (chitosan nanoparticles or quantum dots) successfully reduced the tumors size. This study provides some information about the preparation techniques of paclitaxel loaded nanocarriers and some possible alternatives or strategies for the treatment of breast cancer using these nanocarriers.
Journal of Ethnopharmacology
Journal of Ethnopharmacology
Saudi Pharmaceutical Journal
Open Chemistry
In Turkish folk medicine, the fruits of Pyrus elaeagnifolia subsp. elaeagnifolia have been used t... more In Turkish folk medicine, the fruits of Pyrus elaeagnifolia subsp. elaeagnifolia have been used to treat diarrhea and detoxify poisonous snake bites by enlarging the wound. The aim of the study was to confirm the ethnopharmacological usage of the plant using in vivo and in vitro models. Experimental colitis was performed under anesthesia by intrarectal administration of acetic acid in rats, and the extracts were administered orally. The colonic malondialdehyde (MDA), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nitrite levels, in addition to the myeloperoxidase (MPO) and caspase-3 activities, were measured to determine the effects of the plant extracts. The methanol (MeOH) extract revealed a significant decrease in MPO and caspase-3 levels. The MeOH extract was found to have the highest total tannin content. It was also found to have significant antioxidant (p ˂ 0.01) and anti-inflammatory activities (p ˂ 0.05) in acetic acid induced colitis rat model . According to our ...
Journal of Ethnopharmacology
Ethnobotanical investigations have shown that the Pinus species have been used against rheumatic ... more Ethnobotanical investigations have shown that the Pinus species have been used against rheumatic pain and for wound healing in Turkish folk medicine. In this study, phytochemical composition, antioxidant, anti-inflammatory, and wound healing activities of Maritime Pine (Pinus pinaster Ait.) that is collected in Turkey are investigated. Essential oil composition and the amount of extracts (lipophilic and hydrophilic) of maritime pine wood and fresh cone samples had been tested. The essential oil from cones of P. pinaster revealed the highest activities, whereas other parts of the plant did not display any appreciable wound healing, anti-inflammatory, or antioxidant effects. α-Pinene was the main constituent of the essential oil obtained from the cones of P. pinaster. Experimental studies shown that P. pinaster&amp;#39;s remarkable anti-inflammatory and wound healing activities support the traditional use of the plant, and suggest it could have a place in modern medicine.
Turkiye Klinikleri Tıp Bilimleri Dergisi, 1996
Turkiye Klinikleri Journal of Medical Sciences, 2004
Turkiye Klinikleri Journal of Medical Sciences, 2008
... ORIGINAL RESEARCH. Sitogenetik Araştırmalarda İnversiyon Analizi Hakkı TAŞTAN, MD a ,Ayla TÜZ... more ... ORIGINAL RESEARCH. Sitogenetik Araştırmalarda İnversiyon Analizi Hakkı TAŞTAN, MD a ,Ayla TÜZÜN, MD a ,Serdar YÜKSEL b a Department of Biology, Ankara University Faculty of Science b Department of Genetic, Dr. Zekai Tahir Burak Women's Hospital's, Ankara. ...
Turkiye Klinikleri Journal of Case Reports, 1998
Gene therapy & molecular biology
Objective: To assess the association between the MTHFR gene (Al298C), MTR gene (A2756G) and MTRR ... more Objective: To assess the association between the MTHFR gene (Al298C), MTR gene (A2756G) and MTRR gene (A66G) polymorphisms and nonsyndromic cleft lip with or without cleft palate(nsCL/P), we investigated patients in the Turkish population. Methodology: Analysis of gene polymorphisms was carried out using polymerase chain reactions and restriction enzyme digestions. Our study included 100 patients with nsCL/P and 125 age matched healty individuals. Results: We found that MTHFR Al298C polymorphism and MTR A2756G polymorphism are significant risk factors for nsCL/P. The frequency of MTRR A66G homozygotes was higher compared to control group, however the difference was not statistically significant. Conclusions: We revealed a statistical significant association between the MTHFR Al298C and MTR A2756G polymorphism and nsCL/P.
Archives of Oral Biology, 2014
Non-syndromic cleft lip with/without cleft palate (nsCL/P) has a complex aetiology involving both... more Non-syndromic cleft lip with/without cleft palate (nsCL/P) has a complex aetiology involving both genetic and environmental factors. The aim of this study was to investigate the association between PVRL1 gene mutations and Turkish patients with nsCL/P. In this study, 80 Turkish patients with nsCL/P and 125 unrealeted individuals were analyzed. Mutations were detected using polymerase chain reactions and DNA sequencing. We found a novel GGA insertion between nucleotide positions c.1311_1313delGGA in exon 6 of the PVRL1 gene. Fifteen of the 80 patients with nsCL/P had the GGA insertion, although no mutation was found in the 125 unrelated individuals. We identified new supportive evidence that the association between PVRL1 gene and nsCL/P.
International Journal of Pediatric Otorhinolaryngology, 2013
Orofacial clefts are common congenital defects, with a birth prevalence of approximately 1/1000 i... more Orofacial clefts are common congenital defects, with a birth prevalence of approximately 1/1000 in Caucasians [1,2]. Prevalence rate varies according to geographical origin [3], sex [4] racial background and ethnicity [5], socioeconomic status [6]. Orofacial clefts are classified based on the presence of other congenital anomalies: nonsyndromic cleft lip with or without cleft palate, nonsyndromic cleft palate only, syndromic cleft lip with or without cleft palate and syndromic cleft palate only about 70% of cleft lip with or without cleft palate cases are nonsyndromic form and syndromic cleft containing part of 30% can be seen together with more than 300 defined mendelian syndromes and uncategorized syndromes [7,8]. Nonsyndromic cleft lip with or without cleft palate (nsCL/P, MIM 119530) is one of the most common birth defects, involving genetic and environmental factors [9-11]. Environmental factors such as cigarette smoking and alcohol consumption, intake antiepileptic drugs, inadequate nutrition especially inadequate folate and B6 B12 intake during pregnancy [12-16]. To identify genetic factors, used linkage and association analyses. Several loci or candidate genes have been identified in nsCL/P by these analyses [11] at the linkage technique, chromosomes of family members with CL/P make comparison by looking at chromosomes of unaffected family members [17]. For association technique, investigations make on only cases with CL/P [7]. There are candidate genes that studied to investigate of CL/P genetically: MTHFR,
Turkiye Klinikleri Journal of Medical Sciences, 2011
The Photogrammetric Record, 2002