hamdy nassar - Academia.edu (original) (raw)
Papers by hamdy nassar
Al-Azhar Journal of Dentistry, 2024
This study aimed to investigate the possible association of bone mineral density with alveolar bo... more This study aimed to investigate the possible association of bone mineral density with alveolar bone loss in women during postmenopause. Patient and methods: Totally 60 women after menopause between the ages of 45 and 65 were divided equally into the following three bands using a dual radiography absorptiometry scan (DXA): 20 participants in group I were healthy (T-score of À1). Cluster II consisted of 20 women during postmenopause with osteopenia (À1 > T-score > À2.5). 20 osteoporotic females with menopause form cohort III (T-score À2.5 or less). The cementoenamel junction and alveolar crest distance CE-JAC were measured using cone-beam computed tomography images. Results: The relationship between clinical attachment loss and linear measures of bone loss at six locations surrounding the most damaged tooth in the maxilla and mandible was favorable in osteopenic and osteoporotic groups (weak to moderate), while negative association in the normal group (poor to mediate). Conclusions: Osteoporosis and periodontal disease were correlated, with the greatest linkage in women who were in post-menopausal stage. So the oral health promotion, including routine dental examination and oral hygiene care, is very beneficial.
Al-Azhar Journal of Dentistry, Dec 31, 2022
Purpose: The present study aimed to examine serum cortisol concentrations and salivary immunoglob... more Purpose: The present study aimed to examine serum cortisol concentrations and salivary immunoglobulin A (S-IgA) levels under psychological stress during the COVID-19 virus threat and its relationship to periodontal condition. Materials and methods: A total of 30 participants who had particularly high scores on the perceived stress scale (PSS) were involved in this study. Psychological stress was assessed via the PSS questionnaire. The Clinical periodontal evaluation included assessment of gingival index (GI), probing depth (PD) and clinical attachment level (CAL). Biochemical quantification of serum cortisol concentrations and S-IgA levels was performed using the enzyme-linked immunosorbent assays method (ELIZA). Every step was performed at the baseline during the lockdown, 3 and 6 months follow up after lockdown. Results: An increase in stress levels was detected over the course of the study and was statistically significant. Clinically, worsening of GI, PD and CAL records was observed over the follow-ups, yet this increase is non-significant for all indices (P>0.05). The correlation between serum cortisol and stress was positive and statistically significant over the three pickups (P<0.05). A weak negative correlation between stress and S-IgA was reported and wasn't statistically significant. Conclusion: The impact of stress caused by lockdown and fear of pandemic was a continuous process where the time factor was very crucial in this study. High cortisol levels as a stress response and consequently S-IgA diminution were associated with worsening of the periodontal condition. Prolonged periods of stress may have an exaggerated impact on the periodontal condition of the subjects.
Al-Azhar Journal of Dentistry
Al-Azhar Journal of Dentistry
Acta odontológica latinoamericana : AOL, 2014
Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim o... more Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascu... more Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascular Endothelial Growth factor (VEGF) biomarker present in (GCF) in response to LLLT combined with Platelet Rich Fibrin (PRF) & bovine xenograft to treat infra-bony defects. Material and methods: The current study was conducted on twenty (20) sites from ten (10) patients. A spilt-mouth design used, sites were randomly selected from each patient, into two groups: laser group; ten (10) sites received PRF system without irradiation, and control group; ten (10) sites received PRF system and it will be irradiated with low level laser. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), Plaque index (PI), and gingival index (GI). ELISA quantification analysis of VEGF was done. Results: comparison between the groups at 6 months showed significant difference between both Probing depth and clinical attachment level, while Plaque index and Gingival index showe...
Journal of the International Academy of Periodontology, 2012
Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone ... more Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone formation. The purpose of this study was to evaluate the effectiveness of different times of initiating the active orthodontic tooth movement on the regenerative potential of the intrabony defects. Fifteen adult patients with at least three intrabony defects and malocclusion were included. A total of 45 defects were divided into three groups and treated in a split mouth design. The defects were treated with combined orthodontic regenerative therapy with immediate application of orthodontic tooth movement or delayed application of orthodontic tooth movement (test groups) or with regenerative therapy alone (control group). The following hard and soft tissue measurements were recorded prior to initial surgery and after six months and one year: probing depth (DP), clinical attachment level (CAL), bone density (BD) and bone fill (BF). The most significant results were greater for the group tr...
Perio J, 2021
Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, m... more Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, mixed with a nanohydroxyapatite graft (NHG) covered by an occlusive resorbable collagen membrane (CM) in the surgical treatment of grade II furcation defects and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), as a marker of oxidative injury. Methods: In this randomized controlled clinical study a total of 24 patients with grade II furcation defects were randomly assigned into three equal groups. Furcation defects in group I were managed with lycopene gel mixed with NHG and CM, group II with NHG and CM, and group III with open flap debridement only. Site-specific changes in clinical parameters including probing depth (PD), vertical clinical attachment level (VCAL), horizontal clinical attachment level (HCAL), radiographic maximum vertical depth (MAX V), and maximum horizontal depth (MAX H) were measured at baseline and six months postoperatively. Gingiva...
Infection and Immunity, 2002
Recent cross-sectional and prospective epidemiological studies have demonstrated an association b... more Recent cross-sectional and prospective epidemiological studies have demonstrated an association between periodontal disease and atherosclerosis and human coronary heart disease. Previously, we have established that the periodontal pathogen Porphyromonas gingivalis is capable of invading aortic, heart, and human umbilical vein endothelial cells (HUVEC). Since atherosclerosis is a chronic inflammatory response initiated at the vascular wall, interactions of P. gingivalis with endothelial cells and the subsequent host cell response to infection may be important in the pathogenesis of atherosclerosis. In this study we examined the consequences of P. gingivalis infection of HUVEC on the expression of the chemokines interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1). HUVEC were found to constitutively produce low levels of IL-8 and MCP-1. The addition of P. gingivalis fimbrillin-specific peptides, lipopolysaccharides (LPS), or heat-killed whole cell preparations to HUVEC stim...
Periodontology 2000, 2007
Al-Azhar Dental Journal for Girls, Apr 1, 2020
Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascu... more Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascular Endothelial Growth factor (VEGF) biomarker present in (GCF) in response to LLLT combined with Platelet Rich Fibrin (PRF) & bovine xenograft to treat infra-bony defects. Material and methods: The current study was conducted on twenty (20) sites from ten (10) patients. A spilt-mouth design used, sites were randomly selected from each patient, into two groups: laser group; ten (10) sites received PRF system without irradiation, and control group; ten (10) sites received PRF system and it will be irradiated with low level laser. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), Plaque index (PI), and gingival index (GI). ELISA quantification analysis of VEGF was done. Results: comparison between the groups at 6 months showed significant difference between both Probing depth and clinical attachment level, while Plaque index and Gingival index showed a significance difference starting from 2 weeks postoperatively. VEGF revealed no significant difference among groups. Conclusion: The using of low intensity laser with PRF system showed an improvement in the outcome of infra bony defects treatment.
BACKGROUND The aim of this study was to histologically evaluate the effectiveness of different ti... more BACKGROUND The aim of this study was to histologically evaluate the effectiveness of different times of initiating orthodontic tooth movement on enhancement of bone formation in surgical bony defects. METHODS In 18 male guinea pigs, 3-4 months of age, a bony defect was created in the alveolar process midway between the central incisor and mandibular 1st molar. These bony defects were implanted with bioactive glass particles and collagen membrane. According to the application of the orthodontic tooth movement, the animals were divided into three groups, each comprised of six guinea pigs. In Group I, the orthodontic tooth movement was initiated immediately after the surgical procedure. In Group II, the orthodontic tooth movement was applied 2 weeks after the surgical procedure, while in Group III no orthodontic tooth movement was applied. Section blocks for histology were made at 1, 3 and 6 weeks after the surgical procedure. RESULTS All experimental sites showed active bone formation...
Purpose: to measure platelet rich fibrin matrix PRFM in enhancing root coverage of Miller class I... more Purpose: to measure platelet rich fibrin matrix PRFM in enhancing root coverage of Miller class I and II gingival recession clinically by coronally advanced flap. Materials and Methods: Eighteen healthy patients were randomly splitted for three groups: group I (Test group): five patients treated using Platelet Rich Fibrin Matrix (PRFM) membrane and coronally advanced flap, group II (Positive Control group): fivepatients treated by collagen membrane and coronally advanced flapand group III (Negative control group):five patients treated only using coronally advanced flap. Recession width(RW), recession depth(RD), and probing pocket depth (PPD)were evaluated at baseline,three months and six months respectively. Results: In comparing all groups at all time intervals recorded in RD and RW, a statistically significant difference was found.A great reduction in PPD were recorded at 6 monthsthan at 3 month (P-value = 0.50). However, this different was statistically insignificant. Conclusion:...
Al-Azhar Dental Journal for Girls
Purpose: to evaluate the effect of PRF system (PRF mixed with bone graft) in the regeneration of ... more Purpose: to evaluate the effect of PRF system (PRF mixed with bone graft) in the regeneration of intra-bony defects by assessing osteocalcin in gingival Crevicular fluid. This study was accomplished on periodontal clinical and Radiographic evaluation. Subjects and Methods: A randomized double blind, split-mouth study was undergone on 12 patients with twenty four intrabony defects the selected defects were randomly divided into Group I (Test group): including eight defects that received PRF mixed with grafting material. Group II (Collagen group): including eight defects that received collagen membrane and PRF mixed with grafting material and Group III (Control group): including eight defects that received open flap debridement only. All subjects were put on a recall schedule where the clinical parameters (PI, GI, PD and CAL) and radiographic assessment(Alveolar bone height and bone density) were measured and recorded at base line and six months postoperatively. Results: Clinical outcomes in defects treated with collagen membrane and PRF mixed with grafting material (group II) showed more pocket reduction, greater gain in attachment level compared to other groups. However, better improvement in radiographic parameters was observed in defects treated with PRF mixed with grafting material (group I). Regarding mean osteocalcin concentration, defects treated with collagen membrane and PRF mixed with grafting material (group II) reported maximum reduction at the end of the study. Conclusions: The use of PRF as a regenerative material in managing periodontal intrabony defects resulted in pocket depth reduction, clinical attachment gain and defect fill with comparable results to bone graft and collagen membrane.
Al-Azhar Dental Journal for Girls
Purpose: This study was carried to evaluate and compare the regenerative capacity of PRF mixed wi... more Purpose: This study was carried to evaluate and compare the regenerative capacity of PRF mixed with bioactive glass versus PRF mixed with bioactive glass combined with collagen membrane in treatment of intrabony defects. This assessment was based on TGF-β1 concentration in GCF. Patients and materials: A sample of 36 intra bony defects were randomaly divided into; Group I: bioactive glass mixed with PRF. Group II: collagen membrane with bioactive glass mixed with PRF. Group III: open flap debridement only. The GCF TGF-β1 was analyzed by ELIZA at base line, one and three months postoperatively and evaluate the efficacy of PRF system in intrabony defects clinically and radiographically at base line, one, three and six months. Results: Mean TGF-β1 concentrations were significantly reduced in one and three months post-surgery when compared to baseline. The mean values of TGF-β1 at 3 months was 0.12± 0, 0.4±0.01 and 0.11±0.01 in groups I, II, III respectively. There was a statistically significant reduction in periodontal probing depth that was 6±0.97 and gain in CAL 3.87±2.14 mm both in group II after 6 months. There was a highest bone density increase was18±5.84 in group I. Conclusions: The assessment of cervical TGF-β1 levels is considered to reflect the ongoing processes in the surrounding periodontal tissues. In addition, utilizing PRF mixed with bioactive glass might be considered a promising regenerative therapy.
International surgery
Surgical treatment of pulmonary hydatidosis varies from one country to another. Whereas some repo... more Surgical treatment of pulmonary hydatidosis varies from one country to another. Whereas some reports describe marsupialization of the residual cavity others favor capitonnage (imbrication) to decrease complications and hospital stay. Post operative complications and hospital stay were compared in a retrospective study of 53 patients treated surgically at the American University Hospital over a period of 10 years. Forty eight cysts were treated by evacuation after chemical sterilization. Fifteen were marsupialized and 33 were imbricated. Five were excised with lung parenchyma, 5 were delivered intact and one cyst was treated by lobectomy. There was no postoperative mortality. There was no statistical difference in the hospital stay or the complication rate between the marsupialized and imbricated cases. Both imbrication and marsupialization are equally effective methods of treatment of pulmonary hydatid cysts. Anatomic resection is rarely required.
Journal of the International Academy of Periodontology, 2012
The aim of this study was to histologically evaluate the effectiveness of different times of init... more The aim of this study was to histologically evaluate the effectiveness of different times of initiating orthodontic tooth movement on enhancement of bone formation in surgical bony defects. In 18 male guinea pigs, 3-4 months of age, a bony defect was created in the alveolar process midway between the central incisor and mandibular 1st molar. These bony defects were implanted with bioactive glass particles and collagen membrane. According to the application of the orthodontic tooth movement, the animals were divided into three groups, each comprised of six guinea pigs. In Group I, the orthodontic tooth movement was initiated immediately after the surgical procedure. In Group II, the orthodontic tooth movement was applied 2 weeks after the surgical procedure, while in Group III no orthodontic tooth movement was applied. Section blocks for histology were made at 1, 3 and 6 weeks after the surgical procedure. All experimental sites showed active bone formation with plump osteoblast and ...
Journal of the International Academy of Periodontology, 2012
Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone ... more Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone formation. The purpose of this study was to evaluate the effectiveness of different times of initiating the active orthodontic tooth movement on the regenerative potential of the intrabony defects. Fifteen adult patients with at least three intrabony defects and malocclusion were included. A total of 45 defects were divided into three groups and treated in a split mouth design. The defects were treated with combined orthodontic regenerative therapy with immediate application of orthodontic tooth movement or delayed application of orthodontic tooth movement (test groups) or with regenerative therapy alone (control group). The following hard and soft tissue measurements were recorded prior to initial surgery and after six months and one year: probing depth (DP), clinical attachment level (CAL), bone density (BD) and bone fill (BF). The most significant results were greater for the group tr...
Infection and Immunity, 2002
Porphyromonas gingivalis is an oral pathogen that has recently been associated with chronic infla... more Porphyromonas gingivalis is an oral pathogen that has recently been associated with chronic inflammatory diseases such as atherosclerosis. The strength of the epidemiological associations of P. gingivalis with atherosclerosis can be increased by the demonstration that P. gingivalis can initiate and sustain growth in human vascular cells. We previously established that P. gingivalis can invade aortic, heart, and human umbilical vein endothelial cells (HUVEC), that fimbriae are required for invasion of endothelial cells, and that fimbrillin peptides can induce the expression of the chemokines interleukin 8 and monocyte chemotactic protein. In this study, we examined the expression of surface-associated cell adhesion molecules on endothelial cells in response to P. gingivalis infection by fluorescence-activated cell sorting FACS analysis and confocal microscopy. Coculture of HUVEC with P. gingivalis strain 381 or A7436 resulted in the induction in the expression of intercellular adhesi...
Al-Azhar Journal of Dentistry, 2024
This study aimed to investigate the possible association of bone mineral density with alveolar bo... more This study aimed to investigate the possible association of bone mineral density with alveolar bone loss in women during postmenopause. Patient and methods: Totally 60 women after menopause between the ages of 45 and 65 were divided equally into the following three bands using a dual radiography absorptiometry scan (DXA): 20 participants in group I were healthy (T-score of À1). Cluster II consisted of 20 women during postmenopause with osteopenia (À1 > T-score > À2.5). 20 osteoporotic females with menopause form cohort III (T-score À2.5 or less). The cementoenamel junction and alveolar crest distance CE-JAC were measured using cone-beam computed tomography images. Results: The relationship between clinical attachment loss and linear measures of bone loss at six locations surrounding the most damaged tooth in the maxilla and mandible was favorable in osteopenic and osteoporotic groups (weak to moderate), while negative association in the normal group (poor to mediate). Conclusions: Osteoporosis and periodontal disease were correlated, with the greatest linkage in women who were in post-menopausal stage. So the oral health promotion, including routine dental examination and oral hygiene care, is very beneficial.
Al-Azhar Journal of Dentistry, Dec 31, 2022
Purpose: The present study aimed to examine serum cortisol concentrations and salivary immunoglob... more Purpose: The present study aimed to examine serum cortisol concentrations and salivary immunoglobulin A (S-IgA) levels under psychological stress during the COVID-19 virus threat and its relationship to periodontal condition. Materials and methods: A total of 30 participants who had particularly high scores on the perceived stress scale (PSS) were involved in this study. Psychological stress was assessed via the PSS questionnaire. The Clinical periodontal evaluation included assessment of gingival index (GI), probing depth (PD) and clinical attachment level (CAL). Biochemical quantification of serum cortisol concentrations and S-IgA levels was performed using the enzyme-linked immunosorbent assays method (ELIZA). Every step was performed at the baseline during the lockdown, 3 and 6 months follow up after lockdown. Results: An increase in stress levels was detected over the course of the study and was statistically significant. Clinically, worsening of GI, PD and CAL records was observed over the follow-ups, yet this increase is non-significant for all indices (P>0.05). The correlation between serum cortisol and stress was positive and statistically significant over the three pickups (P<0.05). A weak negative correlation between stress and S-IgA was reported and wasn't statistically significant. Conclusion: The impact of stress caused by lockdown and fear of pandemic was a continuous process where the time factor was very crucial in this study. High cortisol levels as a stress response and consequently S-IgA diminution were associated with worsening of the periodontal condition. Prolonged periods of stress may have an exaggerated impact on the periodontal condition of the subjects.
Al-Azhar Journal of Dentistry
Al-Azhar Journal of Dentistry
Acta odontológica latinoamericana : AOL, 2014
Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim o... more Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascu... more Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascular Endothelial Growth factor (VEGF) biomarker present in (GCF) in response to LLLT combined with Platelet Rich Fibrin (PRF) & bovine xenograft to treat infra-bony defects. Material and methods: The current study was conducted on twenty (20) sites from ten (10) patients. A spilt-mouth design used, sites were randomly selected from each patient, into two groups: laser group; ten (10) sites received PRF system without irradiation, and control group; ten (10) sites received PRF system and it will be irradiated with low level laser. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), Plaque index (PI), and gingival index (GI). ELISA quantification analysis of VEGF was done. Results: comparison between the groups at 6 months showed significant difference between both Probing depth and clinical attachment level, while Plaque index and Gingival index showe...
Journal of the International Academy of Periodontology, 2012
Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone ... more Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone formation. The purpose of this study was to evaluate the effectiveness of different times of initiating the active orthodontic tooth movement on the regenerative potential of the intrabony defects. Fifteen adult patients with at least three intrabony defects and malocclusion were included. A total of 45 defects were divided into three groups and treated in a split mouth design. The defects were treated with combined orthodontic regenerative therapy with immediate application of orthodontic tooth movement or delayed application of orthodontic tooth movement (test groups) or with regenerative therapy alone (control group). The following hard and soft tissue measurements were recorded prior to initial surgery and after six months and one year: probing depth (DP), clinical attachment level (CAL), bone density (BD) and bone fill (BF). The most significant results were greater for the group tr...
Perio J, 2021
Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, m... more Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, mixed with a nanohydroxyapatite graft (NHG) covered by an occlusive resorbable collagen membrane (CM) in the surgical treatment of grade II furcation defects and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), as a marker of oxidative injury. Methods: In this randomized controlled clinical study a total of 24 patients with grade II furcation defects were randomly assigned into three equal groups. Furcation defects in group I were managed with lycopene gel mixed with NHG and CM, group II with NHG and CM, and group III with open flap debridement only. Site-specific changes in clinical parameters including probing depth (PD), vertical clinical attachment level (VCAL), horizontal clinical attachment level (HCAL), radiographic maximum vertical depth (MAX V), and maximum horizontal depth (MAX H) were measured at baseline and six months postoperatively. Gingiva...
Infection and Immunity, 2002
Recent cross-sectional and prospective epidemiological studies have demonstrated an association b... more Recent cross-sectional and prospective epidemiological studies have demonstrated an association between periodontal disease and atherosclerosis and human coronary heart disease. Previously, we have established that the periodontal pathogen Porphyromonas gingivalis is capable of invading aortic, heart, and human umbilical vein endothelial cells (HUVEC). Since atherosclerosis is a chronic inflammatory response initiated at the vascular wall, interactions of P. gingivalis with endothelial cells and the subsequent host cell response to infection may be important in the pathogenesis of atherosclerosis. In this study we examined the consequences of P. gingivalis infection of HUVEC on the expression of the chemokines interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1). HUVEC were found to constitutively produce low levels of IL-8 and MCP-1. The addition of P. gingivalis fimbrillin-specific peptides, lipopolysaccharides (LPS), or heat-killed whole cell preparations to HUVEC stim...
Periodontology 2000, 2007
Al-Azhar Dental Journal for Girls, Apr 1, 2020
Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascu... more Purpose: The current clinical study was done to examine clinical and biochemical changes of Vascular Endothelial Growth factor (VEGF) biomarker present in (GCF) in response to LLLT combined with Platelet Rich Fibrin (PRF) & bovine xenograft to treat infra-bony defects. Material and methods: The current study was conducted on twenty (20) sites from ten (10) patients. A spilt-mouth design used, sites were randomly selected from each patient, into two groups: laser group; ten (10) sites received PRF system without irradiation, and control group; ten (10) sites received PRF system and it will be irradiated with low level laser. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), Plaque index (PI), and gingival index (GI). ELISA quantification analysis of VEGF was done. Results: comparison between the groups at 6 months showed significant difference between both Probing depth and clinical attachment level, while Plaque index and Gingival index showed a significance difference starting from 2 weeks postoperatively. VEGF revealed no significant difference among groups. Conclusion: The using of low intensity laser with PRF system showed an improvement in the outcome of infra bony defects treatment.
BACKGROUND The aim of this study was to histologically evaluate the effectiveness of different ti... more BACKGROUND The aim of this study was to histologically evaluate the effectiveness of different times of initiating orthodontic tooth movement on enhancement of bone formation in surgical bony defects. METHODS In 18 male guinea pigs, 3-4 months of age, a bony defect was created in the alveolar process midway between the central incisor and mandibular 1st molar. These bony defects were implanted with bioactive glass particles and collagen membrane. According to the application of the orthodontic tooth movement, the animals were divided into three groups, each comprised of six guinea pigs. In Group I, the orthodontic tooth movement was initiated immediately after the surgical procedure. In Group II, the orthodontic tooth movement was applied 2 weeks after the surgical procedure, while in Group III no orthodontic tooth movement was applied. Section blocks for histology were made at 1, 3 and 6 weeks after the surgical procedure. RESULTS All experimental sites showed active bone formation...
Purpose: to measure platelet rich fibrin matrix PRFM in enhancing root coverage of Miller class I... more Purpose: to measure platelet rich fibrin matrix PRFM in enhancing root coverage of Miller class I and II gingival recession clinically by coronally advanced flap. Materials and Methods: Eighteen healthy patients were randomly splitted for three groups: group I (Test group): five patients treated using Platelet Rich Fibrin Matrix (PRFM) membrane and coronally advanced flap, group II (Positive Control group): fivepatients treated by collagen membrane and coronally advanced flapand group III (Negative control group):five patients treated only using coronally advanced flap. Recession width(RW), recession depth(RD), and probing pocket depth (PPD)were evaluated at baseline,three months and six months respectively. Results: In comparing all groups at all time intervals recorded in RD and RW, a statistically significant difference was found.A great reduction in PPD were recorded at 6 monthsthan at 3 month (P-value = 0.50). However, this different was statistically insignificant. Conclusion:...
Al-Azhar Dental Journal for Girls
Purpose: to evaluate the effect of PRF system (PRF mixed with bone graft) in the regeneration of ... more Purpose: to evaluate the effect of PRF system (PRF mixed with bone graft) in the regeneration of intra-bony defects by assessing osteocalcin in gingival Crevicular fluid. This study was accomplished on periodontal clinical and Radiographic evaluation. Subjects and Methods: A randomized double blind, split-mouth study was undergone on 12 patients with twenty four intrabony defects the selected defects were randomly divided into Group I (Test group): including eight defects that received PRF mixed with grafting material. Group II (Collagen group): including eight defects that received collagen membrane and PRF mixed with grafting material and Group III (Control group): including eight defects that received open flap debridement only. All subjects were put on a recall schedule where the clinical parameters (PI, GI, PD and CAL) and radiographic assessment(Alveolar bone height and bone density) were measured and recorded at base line and six months postoperatively. Results: Clinical outcomes in defects treated with collagen membrane and PRF mixed with grafting material (group II) showed more pocket reduction, greater gain in attachment level compared to other groups. However, better improvement in radiographic parameters was observed in defects treated with PRF mixed with grafting material (group I). Regarding mean osteocalcin concentration, defects treated with collagen membrane and PRF mixed with grafting material (group II) reported maximum reduction at the end of the study. Conclusions: The use of PRF as a regenerative material in managing periodontal intrabony defects resulted in pocket depth reduction, clinical attachment gain and defect fill with comparable results to bone graft and collagen membrane.
Al-Azhar Dental Journal for Girls
Purpose: This study was carried to evaluate and compare the regenerative capacity of PRF mixed wi... more Purpose: This study was carried to evaluate and compare the regenerative capacity of PRF mixed with bioactive glass versus PRF mixed with bioactive glass combined with collagen membrane in treatment of intrabony defects. This assessment was based on TGF-β1 concentration in GCF. Patients and materials: A sample of 36 intra bony defects were randomaly divided into; Group I: bioactive glass mixed with PRF. Group II: collagen membrane with bioactive glass mixed with PRF. Group III: open flap debridement only. The GCF TGF-β1 was analyzed by ELIZA at base line, one and three months postoperatively and evaluate the efficacy of PRF system in intrabony defects clinically and radiographically at base line, one, three and six months. Results: Mean TGF-β1 concentrations were significantly reduced in one and three months post-surgery when compared to baseline. The mean values of TGF-β1 at 3 months was 0.12± 0, 0.4±0.01 and 0.11±0.01 in groups I, II, III respectively. There was a statistically significant reduction in periodontal probing depth that was 6±0.97 and gain in CAL 3.87±2.14 mm both in group II after 6 months. There was a highest bone density increase was18±5.84 in group I. Conclusions: The assessment of cervical TGF-β1 levels is considered to reflect the ongoing processes in the surrounding periodontal tissues. In addition, utilizing PRF mixed with bioactive glass might be considered a promising regenerative therapy.
International surgery
Surgical treatment of pulmonary hydatidosis varies from one country to another. Whereas some repo... more Surgical treatment of pulmonary hydatidosis varies from one country to another. Whereas some reports describe marsupialization of the residual cavity others favor capitonnage (imbrication) to decrease complications and hospital stay. Post operative complications and hospital stay were compared in a retrospective study of 53 patients treated surgically at the American University Hospital over a period of 10 years. Forty eight cysts were treated by evacuation after chemical sterilization. Fifteen were marsupialized and 33 were imbricated. Five were excised with lung parenchyma, 5 were delivered intact and one cyst was treated by lobectomy. There was no postoperative mortality. There was no statistical difference in the hospital stay or the complication rate between the marsupialized and imbricated cases. Both imbrication and marsupialization are equally effective methods of treatment of pulmonary hydatid cysts. Anatomic resection is rarely required.
Journal of the International Academy of Periodontology, 2012
The aim of this study was to histologically evaluate the effectiveness of different times of init... more The aim of this study was to histologically evaluate the effectiveness of different times of initiating orthodontic tooth movement on enhancement of bone formation in surgical bony defects. In 18 male guinea pigs, 3-4 months of age, a bony defect was created in the alveolar process midway between the central incisor and mandibular 1st molar. These bony defects were implanted with bioactive glass particles and collagen membrane. According to the application of the orthodontic tooth movement, the animals were divided into three groups, each comprised of six guinea pigs. In Group I, the orthodontic tooth movement was initiated immediately after the surgical procedure. In Group II, the orthodontic tooth movement was applied 2 weeks after the surgical procedure, while in Group III no orthodontic tooth movement was applied. Section blocks for histology were made at 1, 3 and 6 weeks after the surgical procedure. All experimental sites showed active bone formation with plump osteoblast and ...
Journal of the International Academy of Periodontology, 2012
Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone ... more Combined orthodontic/regenerative therapy can resolve complex clinical problems and enhance bone formation. The purpose of this study was to evaluate the effectiveness of different times of initiating the active orthodontic tooth movement on the regenerative potential of the intrabony defects. Fifteen adult patients with at least three intrabony defects and malocclusion were included. A total of 45 defects were divided into three groups and treated in a split mouth design. The defects were treated with combined orthodontic regenerative therapy with immediate application of orthodontic tooth movement or delayed application of orthodontic tooth movement (test groups) or with regenerative therapy alone (control group). The following hard and soft tissue measurements were recorded prior to initial surgery and after six months and one year: probing depth (DP), clinical attachment level (CAL), bone density (BD) and bone fill (BF). The most significant results were greater for the group tr...
Infection and Immunity, 2002
Porphyromonas gingivalis is an oral pathogen that has recently been associated with chronic infla... more Porphyromonas gingivalis is an oral pathogen that has recently been associated with chronic inflammatory diseases such as atherosclerosis. The strength of the epidemiological associations of P. gingivalis with atherosclerosis can be increased by the demonstration that P. gingivalis can initiate and sustain growth in human vascular cells. We previously established that P. gingivalis can invade aortic, heart, and human umbilical vein endothelial cells (HUVEC), that fimbriae are required for invasion of endothelial cells, and that fimbrillin peptides can induce the expression of the chemokines interleukin 8 and monocyte chemotactic protein. In this study, we examined the expression of surface-associated cell adhesion molecules on endothelial cells in response to P. gingivalis infection by fluorescence-activated cell sorting FACS analysis and confocal microscopy. Coculture of HUVEC with P. gingivalis strain 381 or A7436 resulted in the induction in the expression of intercellular adhesi...