hasan basri - Academia.edu (original) (raw)
Papers by hasan basri
British Journal of Oral & Maxillofacial Surgery, 2003
Variations in the anatomy of the inferior alveolar nerve were seen in 2 of the 20 dissections of ... more Variations in the anatomy of the inferior alveolar nerve were seen in 2 of the 20 dissections of the infratemporal fossa in 10 cadavers. A connecting nerve branch that originated from the auriculotemporal nerve joined the inferior alveolar nerve on both sides. The second part of the maxillary artery passed between the mandibular nerve, the root of the inferior alveolar nerve, and the connecting nerve branch which formed a loop. The maxillary artery seemed to be entrapped. Neurovascular entrapment can cause pain and numbness. Anatomical variations in this region should be kept in mind, particularly in cases of failed treatment of trigeminal neuralgia.
Journal of Cardiac Surgery, 2006
Abstract Background: The aim of this study was to evaluate the frequency of requirement for perma... more Abstract Background: The aim of this study was to evaluate the frequency of requirement for permanent pacing and related risk factors after aortic valve replacement. Methods: Among 465 patients operated between 1994 and 2004, 19(4.1%) patients with a mean age 49.9 ± 17.2 years required the implantation of a permanent pacemaker. Eleven of them were female (57.9%). The main indication was aortic stenosis (89.5%). Severe annular calcification was documented in 78.9% of them, and the aortic valve was bicuspid in 57.9%. Results: Risk factors for permanent pacing after aortic valve replacement (AVR) identified by univariate analysis were female sex, hypertension, preoperative ejection fraction, aortic stenosis, annular calcification, bicuspid aorta, presence of right bundle branch block (RBBB) or left bundle branch block (LBBB), prolonged aortic cross-clamp and perfusion times, and preoperative use of calcium channel blockers. Multivariate analysis showed that female sex (p = 0.01, OR; 5.21, 95% CI: 1.48-18.34), annular calcification (p < 0.001, OR; 0.05, 95% CI: 0.01-0.24), bicuspid aortic valve (p = 0.02, OR; 0.24, 95% CI: 0.07-0.84), presence of RBBB (p = 0.009, OR; 0.03, 95% CI: 0.003-0.44) or LBBB (p = 0.01, OR; 0.13, 95% CI: 0.02-0.69), hypertension (p = 0.03, OR; 0.22, 95%CI: 0.05-0.89), and total perfusion time (p = 0.002, OR; 1.05, 95% CI: 1.01-1.08) were associated risk factors. Conclusion: Irreversible atrioventricular block requiring a permanent pacemaker implantation is an uncommon complication after AVR. Risk factors are annular calcification, bicuspid aorta, female sex, presence of RBBB or LBBB, prolonged total perfusion time, and hypertension.
Plant Cell Reports, 1999
Shoots of orange jessamine (Murraya paniculata) a member of the Rutaceae family flowered in vitro... more Shoots of orange jessamine (Murraya paniculata) a member of the Rutaceae family flowered in vitro on half-strength MT basal medium containing 5% sucrose. The highest percentage (95%) of flowering was obtained on medium supplemented with 0.1 mg l–1 N6-benzyladenine and pH 5.7. A “floral gradient” was detected among the stem internodes and root segments derived from seedlings, with shoot and flower formation significantly influenced by position on the shoot internodes and root segments. Flower buds originating from shoots derived from seeds but not other tissues developed into normal flowers and produced zygotic embryos.
Journal of Hazardous Materials, 2007
A simple and accurate method was developed for separation and enrichment of trace levels of gold ... more A simple and accurate method was developed for separation and enrichment of trace levels of gold in environmental samples. The method is based on the adsorption of Au(III)-diethyldithiocarbamate complex on Amberlite XAD-2000 resin prior to the analysis of gold by flame atomic absorption spectrometry after elution with 1 mol L −1 HNO 3 in acetone. Some parameters including nitric acid concentration, eluent type, matrix ions, sample volume, sample flow rate and adsorption capacity were investigated on the recovery of gold(III). The recovery values for gold(III) and detection limit of gold were greater than 95% and 16.6 g L −1 , respectively. The preconcentration factor was 200. The relative standard deviation of the method was <6%. The adsorption capacity of the resin was 12.3 mg g −1 . The validation of the presented procedure was checked by the analysis of CRM-SA-C Sandy Soil certified reference material. The presented procedure was applied to the determination of gold in some environmental samples.
Surgical and Radiologic Anatomy, 2000
Bilateral insertion abnormality of pectoralis minimus (sterno-costo- coracoidian muscle) muscle w... more Bilateral insertion abnormality of pectoralis minimus (sterno-costo- coracoidian muscle) muscle was examined. The variant muscle was lying under the pectoralis major muscle and was medial to the pectoralis minor muscle. This muscle started from the first costal cartilage to the manubrium sterni and ended in the upper surface of the shoulder joint on the right side. On the opposite side, it took origin from the second costal cartilage to the manubrium sterni and the second costochondral joint, afterwards became a tendinous structure and divided into two on the coracoid process. The thicker part ended on the upper surface of the articular capsule of the shoulder joint, the thinner part inserted on the lateral third of inferior part of clavicle and fascia of subclavius muscle.
Journal of Pharmacological Sciences, 2007
Preliminary clinical studies of testosterone therapy in male patients with coronary artery diseas... more Preliminary clinical studies of testosterone therapy in male patients with coronary artery disease obtained promising results. However, little is known about the in vitro effects of testosterone in human isolated arteries. We investigated the effect of testosterone on contractile tone of human isolated radial artery (RA). Testosterone was added (0.1 -300 µM ) cumulatively to organ baths after precontraction with KCl (45 mM) and phenylephrine (PE, 10 µM). Testosterone-induced relaxations were tested in the presence of the cyclooxygenase inhibitor indomethacin (10 µM), nitric oxide synthase inhibitor N ω -nitro-L-arginine methyl ester (L-NAME, 100 µM), non-selective large conductance Ca 2+ -activated and voltage-sensitive K + channel inhibitor tetraethylammonium (TEA, 1 mM), ATP-sensitive K + channel inhibitor glibenclamide (GLI, 10 µM), and voltage-sensitive K + channel inhibitor 4-aminopyridine (4-AP, 1 mM). Testosterone produced relaxation in human RA (E max : 53.03 ± 2.76% and 66.83 ± 1.97% of KCl and PEinduced contraction, respectively). Except for GLI, the relaxation to testosterone is affected by neither K + channel inhibitors (TEA, BaCl 2 , and 4-AP), L-NAME, nor indomethacin. We report for the first time that supraphysiological concentrations of testosterone induces relaxation in RA. This response may occur in part via ATP-sensitive K + channel opening action.
Plant Cell Reports, 1996
A method for somatic embryogenesis and plantlets formation of the Egyptian date palm (cv. Zaghloo... more A method for somatic embryogenesis and plantlets formation of the Egyptian date palm (cv. Zaghlool) was accomplished. Embryogenic callus was established from shoot -tip explants on Murashige and Skoog (MS) medium supplemented with 10 mg/l dichloro-phenoxyacetic acid (2,4-D) + 3 mg/l dimethylaminopurine (2ip). High frequency of conversion to embryos as well as high number of embryos per explant were observed when MS medium devoid of growth regulators was used. Also, subculturing of obtained embryos on MS-hormone-free medium led to regeneration of plantlets. Decreasing sucrose to 2% obviously improved the embryo germination and plantlets elongation. The highest percentages of survival of transferred plantlets to free -living conditions were registered when a mixture of vermiculite and peat moss (1:1) was used.
Clinical Anatomy, 2005
In a routine dissection of the axillary fossa, a muscle originating from the coracoid process of ... more In a routine dissection of the axillary fossa, a muscle originating from the coracoid process of the scapula and extending to the long head of triceps brachii muscle was observed. The mentioned muscle was adhering to both the triceps brachii muscle and the tendinous part of the latissimus dorsi muscle. This anatomical variation is referred to as axillary arch (Langer's muscle or axillopectoral muscle). The muscle mass was measured 9.6 cm in length and 1.4 cm in width. The accessory muscle can be a reason of an axillary mass and can exert pressure on the neighboring neurovascular bundle or lymph routes; thus, exposing a wide range of symptoms. Therefore, variations of this area should be kept in mind in surgical interventions. Clin. Anat. 18:220–223, 2005. © 2005 Wiley-Liss, Inc.
Background. Brucella endocarditis (BE) is a lethal complication of human brucellosis, which is ra... more Background. Brucella endocarditis (BE) is a lethal complication of human brucellosis, which is rarely seen and hardly described.
Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium... more Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium falciparum malaria. We conducted a hospital-based 28-day in vivo test comparing chloroquine/doxycycline to chloroquine or doxycycline alone for treating P. falciparum and Plasmodium vivax malaria in Irian Jaya, Indonesia. Eighty-nine patients with uncomplicated falciparum malaria were randomized to standard dose chloroquine (n ϭ 30), doxycycline (100 mg every 12 hours [7 days], n ϭ 20), or chloroquine with doxycycline (n ϭ 39); corresponding numbers for vivax malaria (n ϭ 63) were 23, 16, 24. Endpoints were parasite sensitivity (S) or resistance (RI/RII/ RIII). Of the 105 evaluable patients, chloroquine/doxycycline cured (S) 20/22 (90.9% [95% CI 78.9-100%]) patients with P. falciparum malaria; 2/22 (9.1% [0-21%]) were RIII resistant. Doxycycline cured 11/17 (64.7% [42.0-87.4%]) patients, and chloroquine 4/20 (20% [2.5-37.5%]). Against P. vivax, chloroquine/doxycycline cured (S) 12/17 (70.6% [48.9-92.2%]) patients, doxycycline 4/12 (33.3% [6.6-59.9%]), and chloroquine 5/17 (29.4% [7.7-51.1%]).
Transactions of The Royal Society of Tropical Medicine and Hygiene, 1996
In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malar... more In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malaria at Nias Island, in the Indian Ocean near north-western Sumatra, Indonesa. The subjects, 21 indigenous males and females (6-50 years old) infected with >40 asexual blood stage parasites ofP. vivax per ~1 of blood, had mild symptoms or none at all. Seven of these patients had > 100 ng/mL whole blood chloroquine levels before the first supervised dose of chloroquine (3 doses of 10 mg/kg, 10 mg/kg, 5 mg/kg of base given at 24 h intervals). Whole blood chloroquine levels on the last day of dosing confirmed normal absorption (range 413-3248, mean 1141, SD 616 ng/mL). Blood films were examined on days 0,2,4,7,11,14,18,21 and 28 after initiating therapy. Three patients had recurrent asexual l? vivax parasitaemias between days 14 and 18, despite effective levels of chloroquine in whole blood (a100 ng/mL) at the time of recurrence. Resistance to standard chloroquine therapy by P. vivax appeared in 14% of infections among residents of Nias.
Clinical Infectious Diseases, 1999
New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylacti... more New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylactic efficacy of azithromycin against P. falciparum in malaria-immune Kenyans was 83%. We conducted a double-blind, placebo-controlled trial to determine the prophylactic efficacy of azithromycin against multidrug-resistant P. falciparum malaria and chloroquine-resistant Plasmodium vivax malaria in Indonesian adults with limited immunity. After radical cure therapy, 300 randomized subjects received azithromycin (148 subjects, 750-mg loading dose followed by 250 mg/d), placebo (77), or doxycycline (75, 100 mg/d). The end point was slide-proven parasitemia. There were 58 P. falciparum and 29 P. vivax prophylaxis failures over 20 weeks. Using incidence rates, the protective efficacy of azithromycin relative to placebo was 71.6% (95% confidence interval [CI], 50.3 -83.8) against P. falciparum malaria and 98.9% (95% CI, 93.1 -99.9) against P. vivax malaria. Corresponding figures for doxycycline were 96.3% (95% CI, 85.4 -99.6) and 98% (95% CI, 88.0 -99.9), respectively. Daily azithromycin offered excellent protection against P. vivax malaria but modest protection against P. falciparum malaria. Materiel Development Activity and the U.S. Naval Medical Research and Development Command (Department of Defense funding reference; 65807/ were coincidentally reduced [16]. In a malaria prophylaxis trial 849/QG). in Kenyan adults, 250 mg of azithromycin daily had a prophy-Reprints: Publications Office, US NAMRU-2, Box 3, Unit 8132, APO, AP lactic efficacy against P. falciparum malaria based on crude 96520-8132, USA. incidence of 83% (95% CI, 68 -91) [17]. Shropshire, SY1 3HS, United Kingdom (Bobbo1@compuserve.com). Azithromycin can be used by pregnant and breast-feeding Clinical Infectious Diseases 1999;28:74-81
Between January 1998 and December 2000, the Jayapura Provincial Public Hospital in northeastern I... more Between January 1998 and December 2000, the Jayapura Provincial Public Hospital in northeastern Indonesian New Guinea (Papua) admitted 5,936 patients with a diagnosis of malaria. The microscopic diagnosis at admission was Plasmodium falciparum (3,976, 67%), Plasmodium vivax (1,135, 19%), Plasmodium malariae (8, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 1%), and mixed species infections (817, 14%). Approximately 9% (367) of patients were classified as having severe malaria (277 P. falciparum, 36 P. vivax, 53 mixed infections, and 1 P. malariae) and 88 died (79 P. falciparum/mixed infections and 9 P. vivax). Risk of fatal outcomes among severe malaria patients was indistinguishable between those with falciparum versus vivax malaria (OR = 0.89; P = 0.771). Compared with non-pregnant women, pregnant women showed no higher risk of severe malaria (P = 0.643) or death caused by severe malaria (P = 0.748). This study compares admissions per population (based on census data), parasitemia, morbidity, and mortality among children versus adults, pregnant versus non-pregnant women, and urban/suburban versus rural residents.
Clinical Infectious Diseases, 2001
Malaria causes illness or death in unprotected travelers. Primaquine prevents malaria by attackin... more Malaria causes illness or death in unprotected travelers. Primaquine prevents malaria by attacking liver-stage parasites, a property distinguishing it from most chemoprophylactics and obviating 4-week postexposure dosing. A daily adult regimen of 30 mg primaquine prevented malaria caused by Plasmodium falciparum and P. vivax for 20 weeks in 95 of 97 glucose-6-phosphate dehydrogenase (G6PD)-normal Javanese transmigrants in Papua, Indonesia. In comparison, 37 of 149 subjects taking placebo in a parallel trial became parasitemic. The protective efficacy of primaquine against malaria was 93% (95% confidence interval [CI] 71%-98%); against P. falciparum it was 88% (95% CI 48%-97%), and &amp;gt;92% for P. vivax (95% CI &amp;gt;37%-99%). Primaquine was as well tolerated as placebo. Mild methemoglobinemia (mean of 3.4%) returned to normal within 2 weeks. Blood chemistry and hematological parameters revealed no evidence of toxicity. Good safety, tolerance, and efficacy, along with key advantages in dosing requirements, make primaquine an excellent drug for preventing malaria in nonpregnant, G6PD-normal travelers.
Lancet, 1995
Drug resistance has made malaria prevention difficult and the new agents are too expensive for wi... more Drug resistance has made malaria prevention difficult and the new agents are too expensive for widespread use. Primaquine, an established drug for treatment, is potentially useful for prevention. Malaria prophylaxis with primaquine was evaluated in Irian Jaya during one year in Javanese men who were not deficient in glucose-6phosphate dehydrogenase (G-6-PD). 126 volunteers were randomised to receive 0·5 mg/kg primaquine base or placebo daily (double-blinded), or 300 mg chloroquine base weekly (open).
Annals of Tropical Medicine and Parasitology, 2003
The epidemiology of infection by Plasmodium falciparum and P. vivax was investigated among Javane... more The epidemiology of infection by Plasmodium falciparum and P. vivax was investigated among Javanese migrants to an endemic region of Papua, Indonesia. A cohort of 243 migrants from Java was followed for malaria in a new settlement village in the endemic Armopa area of north-eastern Papua, beginning on the day each migrant arrived in the village. The subjects were monitored during home visits (three/week) and by the twice-monthly production of bloodsmears that were checked for malarial parasites. At the end of 33 months, 159 (65%) of the subjects remained under follow-up. The prevalence of parasitaemia in the village declined from 16% among those already living there when the study began in August 1996, to 5% when the study finished in June 1999. Over this period, 596 infections by P. falciparum and 723 by P. vivax occurred in the cohort, 22 and 27 of the subjects each experiencing at least six infections by P. falciparum and P. vivax, respectively. The incidence of malarial infection was higher during the first and second years post-migration (3.2 and 2.7 infections/person-year) than during the third (1.2 infections/person-year). Although the geometric mean parasite counts for P. falciparum increased over time (1209, 1478, and 1830 parasites/microl in the first, second and third years, respectively), the corresponding values for P. vivax (497, 535 and 490 parasites/microl) showed no such trend. Only one of the nine subjects who developed severe malaria (requiring intravenous quinine therapy) was a child, giving an odds ratio for a case of severe malaria being in an adult of 6.1 (P=0.08).
Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium... more Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium falciparum malaria. We conducted a hospital-based 28-day in vivo test comparing chloroquine/doxycycline to chloroquine or doxycycline alone for treating P. falciparum and Plasmodium vivax malaria in Irian Jaya, Indonesia. Eighty-nine patients with uncomplicated falciparum malaria were randomized to standard dose chloroquine (n ϭ 30), doxycycline (100 mg every 12 hours [7 days], n ϭ 20), or chloroquine with doxycycline (n ϭ 39); corresponding numbers for vivax malaria (n ϭ 63) were 23, 16, 24. Endpoints were parasite sensitivity (S) or resistance (RI/RII/ RIII). Of the 105 evaluable patients, chloroquine/doxycycline cured (S) 20/22 (90.9% [95% CI 78.9-100%]) patients with P. falciparum malaria; 2/22 (9.1% [0-21%]) were RIII resistant. Doxycycline cured 11/17 (64.7% [42.0-87.4%]) patients, and chloroquine 4/20 (20% [2.5-37.5%]). Against P. vivax, chloroquine/doxycycline cured (S) 12/17 (70.6% [48.9-92.2%]) patients, doxycycline 4/12 (33.3% [6.6-59.9%]), and chloroquine 5/17 (29.4% [7.7-51.1%]).
Transactions of The Royal Society of Tropical Medicine and Hygiene, 1996
In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malar... more In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malaria at Nias Island, in the Indian Ocean near north-western Sumatra, Indonesa. The subjects, 21 indigenous males and females (6-50 years old) infected with >40 asexual blood stage parasites ofP. vivax per ~1 of blood, had mild symptoms or none at all. Seven of these patients had > 100 ng/mL whole blood chloroquine levels before the first supervised dose of chloroquine (3 doses of 10 mg/kg, 10 mg/kg, 5 mg/kg of base given at 24 h intervals). Whole blood chloroquine levels on the last day of dosing confirmed normal absorption (range 413-3248, mean 1141, SD 616 ng/mL). Blood films were examined on days 0,2,4,7,11,14,18,21 and 28 after initiating therapy. Three patients had recurrent asexual l? vivax parasitaemias between days 14 and 18, despite effective levels of chloroquine in whole blood (a100 ng/mL) at the time of recurrence. Resistance to standard chloroquine therapy by P. vivax appeared in 14% of infections among residents of Nias.
Clinical Infectious Diseases, 1999
New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylacti... more New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylactic efficacy of azithromycin against P. falciparum in malaria-immune Kenyans was 83%. We conducted a double-blind, placebo-controlled trial to determine the prophylactic efficacy of azithromycin against multidrug-resistant P. falciparum malaria and chloroquine-resistant Plasmodium vivax malaria in Indonesian adults with limited immunity. After radical cure therapy, 300 randomized subjects received azithromycin (148 subjects, 750-mg loading dose followed by 250 mg/d), placebo (77), or doxycycline (75, 100 mg/d). The end point was slide-proven parasitemia. There were 58 P. falciparum and 29 P. vivax prophylaxis failures over 20 weeks. Using incidence rates, the protective efficacy of azithromycin relative to placebo was 71.6% (95% confidence interval [CI], 50.3 -83.8) against P. falciparum malaria and 98.9% (95% CI, 93.1 -99.9) against P. vivax malaria. Corresponding figures for doxycycline were 96.3% (95% CI, 85.4 -99.6) and 98% (95% CI, 88.0 -99.9), respectively. Daily azithromycin offered excellent protection against P. vivax malaria but modest protection against P. falciparum malaria. Materiel Development Activity and the U.S. Naval Medical Research and Development Command (Department of Defense funding reference; 65807/ were coincidentally reduced [16]. In a malaria prophylaxis trial 849/QG). in Kenyan adults, 250 mg of azithromycin daily had a prophy-Reprints: Publications Office, US NAMRU-2, Box 3, Unit 8132, APO, AP lactic efficacy against P. falciparum malaria based on crude 96520-8132, USA. incidence of 83% (95% CI, 68 -91) [17]. Shropshire, SY1 3HS, United Kingdom (Bobbo1@compuserve.com). Azithromycin can be used by pregnant and breast-feeding Clinical Infectious Diseases 1999;28:74-81
British Journal of Oral & Maxillofacial Surgery, 2003
Variations in the anatomy of the inferior alveolar nerve were seen in 2 of the 20 dissections of ... more Variations in the anatomy of the inferior alveolar nerve were seen in 2 of the 20 dissections of the infratemporal fossa in 10 cadavers. A connecting nerve branch that originated from the auriculotemporal nerve joined the inferior alveolar nerve on both sides. The second part of the maxillary artery passed between the mandibular nerve, the root of the inferior alveolar nerve, and the connecting nerve branch which formed a loop. The maxillary artery seemed to be entrapped. Neurovascular entrapment can cause pain and numbness. Anatomical variations in this region should be kept in mind, particularly in cases of failed treatment of trigeminal neuralgia.
Journal of Cardiac Surgery, 2006
Abstract Background: The aim of this study was to evaluate the frequency of requirement for perma... more Abstract Background: The aim of this study was to evaluate the frequency of requirement for permanent pacing and related risk factors after aortic valve replacement. Methods: Among 465 patients operated between 1994 and 2004, 19(4.1%) patients with a mean age 49.9 ± 17.2 years required the implantation of a permanent pacemaker. Eleven of them were female (57.9%). The main indication was aortic stenosis (89.5%). Severe annular calcification was documented in 78.9% of them, and the aortic valve was bicuspid in 57.9%. Results: Risk factors for permanent pacing after aortic valve replacement (AVR) identified by univariate analysis were female sex, hypertension, preoperative ejection fraction, aortic stenosis, annular calcification, bicuspid aorta, presence of right bundle branch block (RBBB) or left bundle branch block (LBBB), prolonged aortic cross-clamp and perfusion times, and preoperative use of calcium channel blockers. Multivariate analysis showed that female sex (p = 0.01, OR; 5.21, 95% CI: 1.48-18.34), annular calcification (p < 0.001, OR; 0.05, 95% CI: 0.01-0.24), bicuspid aortic valve (p = 0.02, OR; 0.24, 95% CI: 0.07-0.84), presence of RBBB (p = 0.009, OR; 0.03, 95% CI: 0.003-0.44) or LBBB (p = 0.01, OR; 0.13, 95% CI: 0.02-0.69), hypertension (p = 0.03, OR; 0.22, 95%CI: 0.05-0.89), and total perfusion time (p = 0.002, OR; 1.05, 95% CI: 1.01-1.08) were associated risk factors. Conclusion: Irreversible atrioventricular block requiring a permanent pacemaker implantation is an uncommon complication after AVR. Risk factors are annular calcification, bicuspid aorta, female sex, presence of RBBB or LBBB, prolonged total perfusion time, and hypertension.
Plant Cell Reports, 1999
Shoots of orange jessamine (Murraya paniculata) a member of the Rutaceae family flowered in vitro... more Shoots of orange jessamine (Murraya paniculata) a member of the Rutaceae family flowered in vitro on half-strength MT basal medium containing 5% sucrose. The highest percentage (95%) of flowering was obtained on medium supplemented with 0.1 mg l–1 N6-benzyladenine and pH 5.7. A “floral gradient” was detected among the stem internodes and root segments derived from seedlings, with shoot and flower formation significantly influenced by position on the shoot internodes and root segments. Flower buds originating from shoots derived from seeds but not other tissues developed into normal flowers and produced zygotic embryos.
Journal of Hazardous Materials, 2007
A simple and accurate method was developed for separation and enrichment of trace levels of gold ... more A simple and accurate method was developed for separation and enrichment of trace levels of gold in environmental samples. The method is based on the adsorption of Au(III)-diethyldithiocarbamate complex on Amberlite XAD-2000 resin prior to the analysis of gold by flame atomic absorption spectrometry after elution with 1 mol L −1 HNO 3 in acetone. Some parameters including nitric acid concentration, eluent type, matrix ions, sample volume, sample flow rate and adsorption capacity were investigated on the recovery of gold(III). The recovery values for gold(III) and detection limit of gold were greater than 95% and 16.6 g L −1 , respectively. The preconcentration factor was 200. The relative standard deviation of the method was <6%. The adsorption capacity of the resin was 12.3 mg g −1 . The validation of the presented procedure was checked by the analysis of CRM-SA-C Sandy Soil certified reference material. The presented procedure was applied to the determination of gold in some environmental samples.
Surgical and Radiologic Anatomy, 2000
Bilateral insertion abnormality of pectoralis minimus (sterno-costo- coracoidian muscle) muscle w... more Bilateral insertion abnormality of pectoralis minimus (sterno-costo- coracoidian muscle) muscle was examined. The variant muscle was lying under the pectoralis major muscle and was medial to the pectoralis minor muscle. This muscle started from the first costal cartilage to the manubrium sterni and ended in the upper surface of the shoulder joint on the right side. On the opposite side, it took origin from the second costal cartilage to the manubrium sterni and the second costochondral joint, afterwards became a tendinous structure and divided into two on the coracoid process. The thicker part ended on the upper surface of the articular capsule of the shoulder joint, the thinner part inserted on the lateral third of inferior part of clavicle and fascia of subclavius muscle.
Journal of Pharmacological Sciences, 2007
Preliminary clinical studies of testosterone therapy in male patients with coronary artery diseas... more Preliminary clinical studies of testosterone therapy in male patients with coronary artery disease obtained promising results. However, little is known about the in vitro effects of testosterone in human isolated arteries. We investigated the effect of testosterone on contractile tone of human isolated radial artery (RA). Testosterone was added (0.1 -300 µM ) cumulatively to organ baths after precontraction with KCl (45 mM) and phenylephrine (PE, 10 µM). Testosterone-induced relaxations were tested in the presence of the cyclooxygenase inhibitor indomethacin (10 µM), nitric oxide synthase inhibitor N ω -nitro-L-arginine methyl ester (L-NAME, 100 µM), non-selective large conductance Ca 2+ -activated and voltage-sensitive K + channel inhibitor tetraethylammonium (TEA, 1 mM), ATP-sensitive K + channel inhibitor glibenclamide (GLI, 10 µM), and voltage-sensitive K + channel inhibitor 4-aminopyridine (4-AP, 1 mM). Testosterone produced relaxation in human RA (E max : 53.03 ± 2.76% and 66.83 ± 1.97% of KCl and PEinduced contraction, respectively). Except for GLI, the relaxation to testosterone is affected by neither K + channel inhibitors (TEA, BaCl 2 , and 4-AP), L-NAME, nor indomethacin. We report for the first time that supraphysiological concentrations of testosterone induces relaxation in RA. This response may occur in part via ATP-sensitive K + channel opening action.
Plant Cell Reports, 1996
A method for somatic embryogenesis and plantlets formation of the Egyptian date palm (cv. Zaghloo... more A method for somatic embryogenesis and plantlets formation of the Egyptian date palm (cv. Zaghlool) was accomplished. Embryogenic callus was established from shoot -tip explants on Murashige and Skoog (MS) medium supplemented with 10 mg/l dichloro-phenoxyacetic acid (2,4-D) + 3 mg/l dimethylaminopurine (2ip). High frequency of conversion to embryos as well as high number of embryos per explant were observed when MS medium devoid of growth regulators was used. Also, subculturing of obtained embryos on MS-hormone-free medium led to regeneration of plantlets. Decreasing sucrose to 2% obviously improved the embryo germination and plantlets elongation. The highest percentages of survival of transferred plantlets to free -living conditions were registered when a mixture of vermiculite and peat moss (1:1) was used.
Clinical Anatomy, 2005
In a routine dissection of the axillary fossa, a muscle originating from the coracoid process of ... more In a routine dissection of the axillary fossa, a muscle originating from the coracoid process of the scapula and extending to the long head of triceps brachii muscle was observed. The mentioned muscle was adhering to both the triceps brachii muscle and the tendinous part of the latissimus dorsi muscle. This anatomical variation is referred to as axillary arch (Langer's muscle or axillopectoral muscle). The muscle mass was measured 9.6 cm in length and 1.4 cm in width. The accessory muscle can be a reason of an axillary mass and can exert pressure on the neighboring neurovascular bundle or lymph routes; thus, exposing a wide range of symptoms. Therefore, variations of this area should be kept in mind in surgical interventions. Clin. Anat. 18:220–223, 2005. © 2005 Wiley-Liss, Inc.
Background. Brucella endocarditis (BE) is a lethal complication of human brucellosis, which is ra... more Background. Brucella endocarditis (BE) is a lethal complication of human brucellosis, which is rarely seen and hardly described.
Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium... more Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium falciparum malaria. We conducted a hospital-based 28-day in vivo test comparing chloroquine/doxycycline to chloroquine or doxycycline alone for treating P. falciparum and Plasmodium vivax malaria in Irian Jaya, Indonesia. Eighty-nine patients with uncomplicated falciparum malaria were randomized to standard dose chloroquine (n ϭ 30), doxycycline (100 mg every 12 hours [7 days], n ϭ 20), or chloroquine with doxycycline (n ϭ 39); corresponding numbers for vivax malaria (n ϭ 63) were 23, 16, 24. Endpoints were parasite sensitivity (S) or resistance (RI/RII/ RIII). Of the 105 evaluable patients, chloroquine/doxycycline cured (S) 20/22 (90.9% [95% CI 78.9-100%]) patients with P. falciparum malaria; 2/22 (9.1% [0-21%]) were RIII resistant. Doxycycline cured 11/17 (64.7% [42.0-87.4%]) patients, and chloroquine 4/20 (20% [2.5-37.5%]). Against P. vivax, chloroquine/doxycycline cured (S) 12/17 (70.6% [48.9-92.2%]) patients, doxycycline 4/12 (33.3% [6.6-59.9%]), and chloroquine 5/17 (29.4% [7.7-51.1%]).
Transactions of The Royal Society of Tropical Medicine and Hygiene, 1996
In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malar... more In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malaria at Nias Island, in the Indian Ocean near north-western Sumatra, Indonesa. The subjects, 21 indigenous males and females (6-50 years old) infected with >40 asexual blood stage parasites ofP. vivax per ~1 of blood, had mild symptoms or none at all. Seven of these patients had > 100 ng/mL whole blood chloroquine levels before the first supervised dose of chloroquine (3 doses of 10 mg/kg, 10 mg/kg, 5 mg/kg of base given at 24 h intervals). Whole blood chloroquine levels on the last day of dosing confirmed normal absorption (range 413-3248, mean 1141, SD 616 ng/mL). Blood films were examined on days 0,2,4,7,11,14,18,21 and 28 after initiating therapy. Three patients had recurrent asexual l? vivax parasitaemias between days 14 and 18, despite effective levels of chloroquine in whole blood (a100 ng/mL) at the time of recurrence. Resistance to standard chloroquine therapy by P. vivax appeared in 14% of infections among residents of Nias.
Clinical Infectious Diseases, 1999
New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylacti... more New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylactic efficacy of azithromycin against P. falciparum in malaria-immune Kenyans was 83%. We conducted a double-blind, placebo-controlled trial to determine the prophylactic efficacy of azithromycin against multidrug-resistant P. falciparum malaria and chloroquine-resistant Plasmodium vivax malaria in Indonesian adults with limited immunity. After radical cure therapy, 300 randomized subjects received azithromycin (148 subjects, 750-mg loading dose followed by 250 mg/d), placebo (77), or doxycycline (75, 100 mg/d). The end point was slide-proven parasitemia. There were 58 P. falciparum and 29 P. vivax prophylaxis failures over 20 weeks. Using incidence rates, the protective efficacy of azithromycin relative to placebo was 71.6% (95% confidence interval [CI], 50.3 -83.8) against P. falciparum malaria and 98.9% (95% CI, 93.1 -99.9) against P. vivax malaria. Corresponding figures for doxycycline were 96.3% (95% CI, 85.4 -99.6) and 98% (95% CI, 88.0 -99.9), respectively. Daily azithromycin offered excellent protection against P. vivax malaria but modest protection against P. falciparum malaria. Materiel Development Activity and the U.S. Naval Medical Research and Development Command (Department of Defense funding reference; 65807/ were coincidentally reduced [16]. In a malaria prophylaxis trial 849/QG). in Kenyan adults, 250 mg of azithromycin daily had a prophy-Reprints: Publications Office, US NAMRU-2, Box 3, Unit 8132, APO, AP lactic efficacy against P. falciparum malaria based on crude 96520-8132, USA. incidence of 83% (95% CI, 68 -91) [17]. Shropshire, SY1 3HS, United Kingdom (Bobbo1@compuserve.com). Azithromycin can be used by pregnant and breast-feeding Clinical Infectious Diseases 1999;28:74-81
Between January 1998 and December 2000, the Jayapura Provincial Public Hospital in northeastern I... more Between January 1998 and December 2000, the Jayapura Provincial Public Hospital in northeastern Indonesian New Guinea (Papua) admitted 5,936 patients with a diagnosis of malaria. The microscopic diagnosis at admission was Plasmodium falciparum (3,976, 67%), Plasmodium vivax (1,135, 19%), Plasmodium malariae (8, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 1%), and mixed species infections (817, 14%). Approximately 9% (367) of patients were classified as having severe malaria (277 P. falciparum, 36 P. vivax, 53 mixed infections, and 1 P. malariae) and 88 died (79 P. falciparum/mixed infections and 9 P. vivax). Risk of fatal outcomes among severe malaria patients was indistinguishable between those with falciparum versus vivax malaria (OR = 0.89; P = 0.771). Compared with non-pregnant women, pregnant women showed no higher risk of severe malaria (P = 0.643) or death caused by severe malaria (P = 0.748). This study compares admissions per population (based on census data), parasitemia, morbidity, and mortality among children versus adults, pregnant versus non-pregnant women, and urban/suburban versus rural residents.
Clinical Infectious Diseases, 2001
Malaria causes illness or death in unprotected travelers. Primaquine prevents malaria by attackin... more Malaria causes illness or death in unprotected travelers. Primaquine prevents malaria by attacking liver-stage parasites, a property distinguishing it from most chemoprophylactics and obviating 4-week postexposure dosing. A daily adult regimen of 30 mg primaquine prevented malaria caused by Plasmodium falciparum and P. vivax for 20 weeks in 95 of 97 glucose-6-phosphate dehydrogenase (G6PD)-normal Javanese transmigrants in Papua, Indonesia. In comparison, 37 of 149 subjects taking placebo in a parallel trial became parasitemic. The protective efficacy of primaquine against malaria was 93% (95% confidence interval [CI] 71%-98%); against P. falciparum it was 88% (95% CI 48%-97%), and &amp;gt;92% for P. vivax (95% CI &amp;gt;37%-99%). Primaquine was as well tolerated as placebo. Mild methemoglobinemia (mean of 3.4%) returned to normal within 2 weeks. Blood chemistry and hematological parameters revealed no evidence of toxicity. Good safety, tolerance, and efficacy, along with key advantages in dosing requirements, make primaquine an excellent drug for preventing malaria in nonpregnant, G6PD-normal travelers.
Lancet, 1995
Drug resistance has made malaria prevention difficult and the new agents are too expensive for wi... more Drug resistance has made malaria prevention difficult and the new agents are too expensive for widespread use. Primaquine, an established drug for treatment, is potentially useful for prevention. Malaria prophylaxis with primaquine was evaluated in Irian Jaya during one year in Javanese men who were not deficient in glucose-6phosphate dehydrogenase (G-6-PD). 126 volunteers were randomised to receive 0·5 mg/kg primaquine base or placebo daily (double-blinded), or 300 mg chloroquine base weekly (open).
Annals of Tropical Medicine and Parasitology, 2003
The epidemiology of infection by Plasmodium falciparum and P. vivax was investigated among Javane... more The epidemiology of infection by Plasmodium falciparum and P. vivax was investigated among Javanese migrants to an endemic region of Papua, Indonesia. A cohort of 243 migrants from Java was followed for malaria in a new settlement village in the endemic Armopa area of north-eastern Papua, beginning on the day each migrant arrived in the village. The subjects were monitored during home visits (three/week) and by the twice-monthly production of bloodsmears that were checked for malarial parasites. At the end of 33 months, 159 (65%) of the subjects remained under follow-up. The prevalence of parasitaemia in the village declined from 16% among those already living there when the study began in August 1996, to 5% when the study finished in June 1999. Over this period, 596 infections by P. falciparum and 723 by P. vivax occurred in the cohort, 22 and 27 of the subjects each experiencing at least six infections by P. falciparum and P. vivax, respectively. The incidence of malarial infection was higher during the first and second years post-migration (3.2 and 2.7 infections/person-year) than during the third (1.2 infections/person-year). Although the geometric mean parasite counts for P. falciparum increased over time (1209, 1478, and 1830 parasites/microl in the first, second and third years, respectively), the corresponding values for P. vivax (497, 535 and 490 parasites/microl) showed no such trend. Only one of the nine subjects who developed severe malaria (requiring intravenous quinine therapy) was a child, giving an odds ratio for a case of severe malaria being in an adult of 6.1 (P=0.08).
Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium... more Combination therapy is one method of overcoming the global challenge of drug-resistant Plasmodium falciparum malaria. We conducted a hospital-based 28-day in vivo test comparing chloroquine/doxycycline to chloroquine or doxycycline alone for treating P. falciparum and Plasmodium vivax malaria in Irian Jaya, Indonesia. Eighty-nine patients with uncomplicated falciparum malaria were randomized to standard dose chloroquine (n ϭ 30), doxycycline (100 mg every 12 hours [7 days], n ϭ 20), or chloroquine with doxycycline (n ϭ 39); corresponding numbers for vivax malaria (n ϭ 63) were 23, 16, 24. Endpoints were parasite sensitivity (S) or resistance (RI/RII/ RIII). Of the 105 evaluable patients, chloroquine/doxycycline cured (S) 20/22 (90.9% [95% CI 78.9-100%]) patients with P. falciparum malaria; 2/22 (9.1% [0-21%]) were RIII resistant. Doxycycline cured 11/17 (64.7% [42.0-87.4%]) patients, and chloroquine 4/20 (20% [2.5-37.5%]). Against P. vivax, chloroquine/doxycycline cured (S) 12/17 (70.6% [48.9-92.2%]) patients, doxycycline 4/12 (33.3% [6.6-59.9%]), and chloroquine 5/17 (29.4% [7.7-51.1%]).
Transactions of The Royal Society of Tropical Medicine and Hygiene, 1996
In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malar... more In February 1995 we surveyed resistance to chloroquine among patients with Plasmodium vivax malaria at Nias Island, in the Indian Ocean near north-western Sumatra, Indonesa. The subjects, 21 indigenous males and females (6-50 years old) infected with >40 asexual blood stage parasites ofP. vivax per ~1 of blood, had mild symptoms or none at all. Seven of these patients had > 100 ng/mL whole blood chloroquine levels before the first supervised dose of chloroquine (3 doses of 10 mg/kg, 10 mg/kg, 5 mg/kg of base given at 24 h intervals). Whole blood chloroquine levels on the last day of dosing confirmed normal absorption (range 413-3248, mean 1141, SD 616 ng/mL). Blood films were examined on days 0,2,4,7,11,14,18,21 and 28 after initiating therapy. Three patients had recurrent asexual l? vivax parasitaemias between days 14 and 18, despite effective levels of chloroquine in whole blood (a100 ng/mL) at the time of recurrence. Resistance to standard chloroquine therapy by P. vivax appeared in 14% of infections among residents of Nias.
Clinical Infectious Diseases, 1999
New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylacti... more New drugs are needed for preventing drug-resistant Plasmodium falciparum malaria. The prophylactic efficacy of azithromycin against P. falciparum in malaria-immune Kenyans was 83%. We conducted a double-blind, placebo-controlled trial to determine the prophylactic efficacy of azithromycin against multidrug-resistant P. falciparum malaria and chloroquine-resistant Plasmodium vivax malaria in Indonesian adults with limited immunity. After radical cure therapy, 300 randomized subjects received azithromycin (148 subjects, 750-mg loading dose followed by 250 mg/d), placebo (77), or doxycycline (75, 100 mg/d). The end point was slide-proven parasitemia. There were 58 P. falciparum and 29 P. vivax prophylaxis failures over 20 weeks. Using incidence rates, the protective efficacy of azithromycin relative to placebo was 71.6% (95% confidence interval [CI], 50.3 -83.8) against P. falciparum malaria and 98.9% (95% CI, 93.1 -99.9) against P. vivax malaria. Corresponding figures for doxycycline were 96.3% (95% CI, 85.4 -99.6) and 98% (95% CI, 88.0 -99.9), respectively. Daily azithromycin offered excellent protection against P. vivax malaria but modest protection against P. falciparum malaria. Materiel Development Activity and the U.S. Naval Medical Research and Development Command (Department of Defense funding reference; 65807/ were coincidentally reduced [16]. In a malaria prophylaxis trial 849/QG). in Kenyan adults, 250 mg of azithromycin daily had a prophy-Reprints: Publications Office, US NAMRU-2, Box 3, Unit 8132, APO, AP lactic efficacy against P. falciparum malaria based on crude 96520-8132, USA. incidence of 83% (95% CI, 68 -91) [17]. Shropshire, SY1 3HS, United Kingdom (Bobbo1@compuserve.com). Azithromycin can be used by pregnant and breast-feeding Clinical Infectious Diseases 1999;28:74-81