hasan yusufoglu - Academia.edu (original) (raw)

Papers by hasan yusufoglu

Indian Journal of Traditional Knowledge, Nov 4, 2021

Bambusa tulda (Poaceae) is one of the most valuable bamboo species in terms of health-promoting e... more Bambusa tulda (Poaceae) is one of the most valuable bamboo species in terms of health-promoting effects. The goal of this research work was to carry out the in-vivo acute toxicity, anti-diabetic and anti-oxidative activities of hydro-methanolic extract of B. tulda leaves. The median lethal dose (LD50) of the hydro-methanolic extract of B. tulda leaves was found to be 6088.13 mg/kg body weight in mice. Supplementing the low (100 mg/kg) and high dose (200 mg/kg) of B. tulda leaf extract showed significant elevation in the endogenous enzymes level of superoxide dismutase (24.81%) and glutathione peroxidase (31.60%) with a decline in malondialdehyde levels (21.90%) when compared to untreated alloxan-induced diabetic control rats. The histopathological assessment of pancreas also showed an increase in β-cells, though not at a significant level. Hence the presence of phyto-constituents substantiates the pharmacological activities of hydro-methanolic extract of B. tulda leaf particularly as a potential candidate for anti-diabetic activity. However, detailed studies are needed to elucidate its exact mechanism of action against diabetes.

Acta Chromatographica, Dec 28, 2017

In this research, a novel method was developed for the matrix solid phase dispersion (MSPD) follo... more In this research, a novel method was developed for the matrix solid phase dispersion (MSPD) followed by high-performance liquid chromatography (HPLC) quantification of four marker constituents (vitamin C, gallic acid, rutin, and ellagic acid) in the freeze-dried pomegranate fruit juice. Various MSPD parameters like type of dispersant, sample-dispersant ratio, solvents, its volume, and time of extraction have been optimized after many trials. Furthermore, HPLC method has been developed and optimized for the analysis of all four components. The HPLC separation was achieved using a 250 × 4.6 mm column, particle size of 5 μm, C 18 reverse phase column, with a mobile phase consisting of acetonitrile and 0.05% H 3 PO 4 , in gradient elution mode with a mobile phase flow rate of 1 mL/min, using ultraviolet (UV)-visible detection at 254 nm. All calibration curves showed good linear regression (r 2 ≥ 0.9925) within test ranges. The extraction recoveries of the marker constituents analyzed by MSPD methods were found as ranging from 97.5% to 103.5%. From comparing the chromatograms, validation data and other parameters like time, labor, and feasibility, we found that MSPD technique was most suitable for the analysis as compared to conventional liquid-liquid extraction technique.

Journal of Ethnopharmacology, Jul 1, 2020

Several species of Ferula L. genus have been used in traditional Turkish medicine as aphrodisiac ... more Several species of Ferula L. genus have been used in traditional Turkish medicine as aphrodisiac to treat male sexual dysfunction. Especially, roots and oleo gum resin of F. elaeochytris Korovin, F. communis L., F. assa-foetida L. and F. gummosa Boiss. were claimed to be used for aphrodisiac activity, menstrual regulation and treatment of gastric pain in Anatolia. Ferula L. is represented by 23 taxa in Turkey, 13 of which are endemic species. F. huber-morathii Peşmen (FHM), an endemic plant, is popularly known as ''helizan, çağşır''. Aim of the study: This study aimed to isolate sesquiterpenoids from the roots of Ferula huber-morathii (FHM) and to confirm their aphrodisiac potential in male rats. Material and methods: In a preliminary experiment, the effects of aqueous (H 2 O) and chloroform (CHCl 3) extracts of FHM were tested for their potential aphrodisiac activities in male rats. Then, sesquiterpene derivatives were isolated from the active chloroform extract of FHM roots (FHM-R) and characterized (TLC, 1D, 2D NMR, HR-MS and CD). Moreover, some of the isolates with adequate quantities were evaluated for their possible aphrodisiac effects on male rats. Single doses (10 mg/kg BW) of sildenafil citrate (SC, positive control), gummosin, mogoltavidin, deacetylkellerin, ferukrin acetate with kellerin, elaeochytrin-A and ferutinin were administered orally by gavages to male Wistar albino rats. Mount latency (ML), mount frequency (MF), intromission latency (IL), intromission frequency (IF), ejaculation latency (EL) and postejaculatory interval (PEI) were studied. In addition, copulatory efficiency (CE) and intercopulatory efficiency (ICE) were calculated. Results: The preliminary experiment revealed that the chloroform extract was the main source of the active compounds as it showed the higher aphrodisiac activity while the aqueous extract was found to be inactive. Eleven sesquiterpene derivatives, viz. gummosin, mogoltavidin, farnesiferol A, deacetylkellerin, ferukrin acetate, kellerin, teuclatriol, feruhermonin C, ferutinin, elaeochytrin A and teferidin, were isolated from the FHM-CHCl 3 extract. Oral administration of deacetylkellerin, elaeochytrin-A and ferutinin significantly increased MF and IF. The ML and IL were significantly reduced, and ejaculation latencies were prolonged. Administration of these sesquiterpenoids also reduced the PEI. The present results revealed that ferutinin was the most effective aphrodisiac compound compared to other sesquiterpenoids. The results of 10 mg/kg of ferutinin are comparable to SC, the positive control. The results revealed that gummosin, mogoltavidin and ferukrin acetate with kellerin did not significantly alter the aphrodisiac parameters. Conclusions: This study has established that the CHCl 3 extract of FHM root contains sesquiterpene derivatives, especially coumarin ethers and benzoic esters. Findings of the present study demonstrate that the chloroform extract and some of the sesquiterpene derivatives significantly stimulates sexual behavior in male rats, thus suggesting that F. huber-morathii possesses an aphrodisiac activity.

Journal of The Saudi Pharmaceutical Society, Nov 1, 2019

A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been es... more A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone: water: formic acid, 10:6:2:2 (%, v/v/v/v)) on 20 Â 10 cm glass coated silica gel 60 F 254 plates and scanned at 254 nm for ascorbic acid and gallic acid. Ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice were identified by comparing their single spot at R f = 0.54 ± 0.02 and R f = 0.83 ± 0.01 respectively. The value of regression equation (r 2 ! 0.9992) revealed a good linear relationship between peak area and amount of ascorbic acid and gallic acid in the range of 100-800 ng/band. The method was validated for precision, accuracy, robustness LOD and LOQ. The method proposed can be useful for routine determination of ascorbic acid and gallic acid in various crude as well as herbal formulations as a quality control tool.

Pharmaceutical Biology, Dec 23, 2011

Context: Emex spinosa (L.) Campd. (Polygonaceae), Leptadenia pyrotechnica (Forsk.) Decne (Asclepi... more Context: Emex spinosa (L.) Campd. (Polygonaceae), Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae), Haloxylon salicornicum (Moq.) Bunge ex Bioss. (Chenopodiaceae) and Ochradenus baccatus Delile (Resedaceae) are used in folk medicine for treatment of male sexual disorders. Objective: To investigate the effects of E. spinosa, L. pyrotechnica, H. salicornicum and O. baccatus extracts on the reproductive system of male rats after prolonged period of treatment. Methods: Seventy-eight healthy adult male Wistar rats were divided into 13 groups (6 animals, each). The plant extracts (100, 200 and 400 mg/kg) were given daily by gavage to different groups of rats for 65 days. The thirteenth group (control) received the vehicle only. Test and control rats were mated with estrus female rats on days 30, 45 and 60 of treatment. Body and relative reproductive organ weights, and sperm parameters were recorded. Results: Animals treated with the ethanol extracts of E. spinosa and L. pyrotechnica showed significant improvement of the relative weight of reproductive organs, sperm count, sperm motility and total sperm abnormality. The mean sperm count for E. spinosa group (400 mg/kg) was 233.7 ± 4.50 × 10 6 /mL, for L. pyrotechnica (200 and 400 mg/kg) groups were 237.0 ± 5.22 × 10 6 /mL and 240.3 ± 4.64 × 10 6 /mL, respectively and that of the control group was 218.1 ± 4.28 × 10 6 /mL. The sperm motility of the control group was 77.5 ± 2.12, those of E. spinosa (400 mg/kg) group was 87.3 ± 3.50% and those of L. pyrotechnica (200 and 400 mg/kg) groups were 86.0 ± 3.11 and 89.7 ± 2.90%, respectively. Ethanol extracts of E. spinosa (400 mg/kg) and L. pyrotechnica (200 and 400 mg/kg) significantly elevate the serum levels of testosterone (5.30 ± 0.15, 5.32 ± 0.20 and 5.66 ± 0.19 ng/mL, respectively vs 4.64 ± 0.16 ng/mL) and luteinizing hormone (0.69 ± 0.03, 0.70 ± 0.03 and 0.74 ± 0.03 mIU/mL, respectively vs 0.59 ± 0.02 mIU/mL). On the other hand, no alterations were observed in body and relative organ weights, sperm numbers as well as sperm morphology of the male rats after the exposure to the H. salicornicum and O. baccatus extracts for 65 days. Conclusions: E. spinosa and L. pyrotechnica extracts appear to possess fertility improvement activity in male rats due to their testosterone increasing property. Moreover, the results suggest the absence of male reproductive toxicity of the H. salicornicum and O. baccatus extracts at tested doses.

Planta Medica, Jul 14, 2014

Pharmacognosy Journal, Apr 11, 2014

The anti-inflammatory and hepatoprotective activitiesof the methanolic extract of Anthemis scrobi... more The anti-inflammatory and hepatoprotective activitiesof the methanolic extract of Anthemis scrobicularis(ANS) herbs were evaluated in rats against carrageenan induced inflammation and carbon tetrachloride (CCl 4)induced hepatic injury. To evaluate the anti-inflammatory effects of ANS, twenty male rats were divided into four equal groups. Injection of 100 μl carrageenan in normal saline into the subplantar region of the hind paw of rats clearly induced paw edema. The volume of paw edema was attenuated following oral administration of ANS. For hepatoprotective effects, twenty five rats were equally divided into five groups.The hepatotoxicity, induced by a single dose of CCl 4 , produced significant (p<0.001) increase of the levels of serumtransaminase, phosphatase, bilirubin and a decrease in proteins were also noticed. The oxidative stress marker such as malondialdehyde (MDA)was increased and nonprotein sulfhydryl (NP-SH) was decreased in the hepatotoxic tissues. Pre-medication of CCl 4-intoxicated rats with ANS at the doses 250 and 500 mg/kg reversed the abnormal liver diagnostic stricture. The results showed that ANS is toxicologically safe when orally administered and possess highly significant anti-inflammatory and hepatoprotective activities and the potentials usefulness of Anthemis scrobicularis in hepatic and inflammatory disease.

Chemistry & Biodiversity, Jul 21, 2022

Tropical Journal of Pharmaceutical Research, Feb 15, 2018

Purpose: To explore the anti-inflammatory and hepatoprotective potentials of Silene villosa Caryo... more Purpose: To explore the anti-inflammatory and hepatoprotective potentials of Silene villosa Caryophyllaceae methanol extract in rats. Methods: Toxicity of S. villosa extract was evaluated in rats. Inflammation was induced in rats by injection of 0.1 mL carrageenan (1 %) in the left hind paws. Carbon tetrachloride (CCl4) was used to induce liver damage. Five groups of rat were used. The 1st (normal control) and 2nd (hepatotoxic) groups received the vehicle. The 3rd, 4th, and 5th groups received silymarin, 250 and 500 mg/kg of S. villosa extract, respectively, for 7 days. Liver injury was induced on the 7th day by intraperitoneal administration of 1 mL/kg of CCl4 to rats in groups 2-5. Results: The results showed that S. villosa is safe. It significantly reduced carrageenan-induced edema compared to normal (p < 0.01) and standard (p < 0.01) groups. The extract protected (p < 0.01) rats against the deleterious effect of CCl4. It decreased (p < 0.01) the elevated serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transferase (γ-GT) and alkaline phosphatase (ALP) as well as elevated serum levels of bilirubin (BRN), compared to CCl4 control rats. Reduced activities of the antioxidant enzymes were significantly increased (p < 0.01) in rat liver, compared with CCl4 control group. The results were confirmed by histological findings in rat liver as the extract reduced necrosis and hydropic degeneration of hepatic tissue compared to CCl4 control group. Conclusion: The results suggest that S. villosa possesses anti-inflammatory and hepatoprotective activities in rats, and therefore, has therapeutic potentials in humans.

Jpc – Journal Of Planar Chromatography – Modern Tlc, Feb 1, 2020

The proposed research study was undertaken to establish a simple, rapid and sensitive high-perfor... more The proposed research study was undertaken to establish a simple, rapid and sensitive high-performance thin-layer chromatography (HPTLC) method for the quantification of trigonelline (TGN) in methanolic, aqueous and hydroalcoholic extracts of different brands of coffee samples with different colours due to roasting time. The proposed HPTLC methodology was carried out using glass coated silica gel 60 F 254 plates. The mobile phase used was quaternary mixture of ethyl acetate:methanol:water:formic acid 5.4:2.3:1.5:0.8 (%, v / v ) which furnished a compact spot of TGN at Rf 0 . 24 ± 0 . 01. The developed plate was scanned and quantified densitometrically at 267 nm. Linear regression analysis presented a good relationship between peak area and amount of TGN in the range of 100–700 ng/band. The method was validated as per the International Conference on Harmonization (ICH) recommendations. The maximum amount of TGN was recorded in roasted Arabic coffee among different brands studied. The amounts of TGN in methanolic, aqueous and hydroalcoholic extract of roasted Arabic coffee were obtained as 0.74, 0.80 and 0.79% w / w , respectively. The proposed analytical methodology will be useful to enumerate the therapeutic dose of TGN in different herbal products.

Journal of pharmaceutical research international, Dec 20, 2019

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condime... more Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants. Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline. Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a

Evidence-based Complementary and Alternative Medicine, Apr 20, 2023

Background. Quercetin hastraditionally been used in various oxidative and urinary tract dysfuncti... more Background. Quercetin hastraditionally been used in various oxidative and urinary tract dysfunctions. Tecurrent project is consequently set to evaluate the defensive efcacy ofQuercetin against potassium bromate (KBrO3) induced testiculartissue oxidative dysfunctions through biochemical, hormonal, and genotoxicmarkers.Methods. To observe theprotective efcacy of Quercetin against urinogenital oxidative dysfunction inrats, thirty six albino male rats were divided into six groups. Protectiveefcacies of Quercetin were checked on reproductive hormonal levels,antioxidant enzyme activities, lipids peroxidation (LP), and DNA damages. Results. Potassium bromate exposure in experimentalanimals caused a reduction in the activities of antioxidant enzymes and disturbedhormonal secretions while enhancing the peroxidation of lipids andfragmentations of DNA. Cotreatment of Quercetin considerably (P<0.01)reversed these abnormalities with admiration to levels of hormones, antioxidantenzymes activities, and peroxidations of lipids secure to those seen inuntreated rats. (P < 0.01)Conclusion. Te fndings of the current project revealedthat various doses of Quercetin are able to keep the testicular organ fromabnormal free radical dysfunctions. Tese improvements might be due to theantioxidant ability of polyphenolic bioactive constituent, i.e., Quercetin.

Applied sciences, Feb 25, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of The Saudi Pharmaceutical Society, Dec 1, 2019

The aim of this study is the development of validated HPTLC method for the quantification of vite... more The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was developed using ethyl acetate:methanol:water:formic acid 30:4:2:1(%, v/v/v/v) on 20 Â 10 cm glass coated silica gel 60 F 254 plates and the developed plate was scanned and quantified densitometrically at k = 340 nm. Linear regression analysis revealed a good linear relationship between peak area and amount of vitexin in the range of 100-700 ng/spot. The amount of vitexin in nine commercial herbal formulations was successfully quantified by the developed HPTLC method. The developed and validated high performance thin layer chromatographic method offers a new sensitive and reliable tool for quantification of vitexinin in various herbal formulations containing Passiflora foetida.

Pharmaceuticals, Aug 31, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

International Journal of Pharmacology, Sep 15, 2018

Planta Medica, Jul 14, 2014

Gels, Feb 24, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of Cosmetic Dermatology, Jan 6, 2023

Healthcare, Sep 13, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Indian Journal of Traditional Knowledge, Nov 4, 2021

Bambusa tulda (Poaceae) is one of the most valuable bamboo species in terms of health-promoting e... more Bambusa tulda (Poaceae) is one of the most valuable bamboo species in terms of health-promoting effects. The goal of this research work was to carry out the in-vivo acute toxicity, anti-diabetic and anti-oxidative activities of hydro-methanolic extract of B. tulda leaves. The median lethal dose (LD50) of the hydro-methanolic extract of B. tulda leaves was found to be 6088.13 mg/kg body weight in mice. Supplementing the low (100 mg/kg) and high dose (200 mg/kg) of B. tulda leaf extract showed significant elevation in the endogenous enzymes level of superoxide dismutase (24.81%) and glutathione peroxidase (31.60%) with a decline in malondialdehyde levels (21.90%) when compared to untreated alloxan-induced diabetic control rats. The histopathological assessment of pancreas also showed an increase in β-cells, though not at a significant level. Hence the presence of phyto-constituents substantiates the pharmacological activities of hydro-methanolic extract of B. tulda leaf particularly as a potential candidate for anti-diabetic activity. However, detailed studies are needed to elucidate its exact mechanism of action against diabetes.

Acta Chromatographica, Dec 28, 2017

In this research, a novel method was developed for the matrix solid phase dispersion (MSPD) follo... more In this research, a novel method was developed for the matrix solid phase dispersion (MSPD) followed by high-performance liquid chromatography (HPLC) quantification of four marker constituents (vitamin C, gallic acid, rutin, and ellagic acid) in the freeze-dried pomegranate fruit juice. Various MSPD parameters like type of dispersant, sample-dispersant ratio, solvents, its volume, and time of extraction have been optimized after many trials. Furthermore, HPLC method has been developed and optimized for the analysis of all four components. The HPLC separation was achieved using a 250 × 4.6 mm column, particle size of 5 μm, C 18 reverse phase column, with a mobile phase consisting of acetonitrile and 0.05% H 3 PO 4 , in gradient elution mode with a mobile phase flow rate of 1 mL/min, using ultraviolet (UV)-visible detection at 254 nm. All calibration curves showed good linear regression (r 2 ≥ 0.9925) within test ranges. The extraction recoveries of the marker constituents analyzed by MSPD methods were found as ranging from 97.5% to 103.5%. From comparing the chromatograms, validation data and other parameters like time, labor, and feasibility, we found that MSPD technique was most suitable for the analysis as compared to conventional liquid-liquid extraction technique.

Journal of Ethnopharmacology, Jul 1, 2020

Several species of Ferula L. genus have been used in traditional Turkish medicine as aphrodisiac ... more Several species of Ferula L. genus have been used in traditional Turkish medicine as aphrodisiac to treat male sexual dysfunction. Especially, roots and oleo gum resin of F. elaeochytris Korovin, F. communis L., F. assa-foetida L. and F. gummosa Boiss. were claimed to be used for aphrodisiac activity, menstrual regulation and treatment of gastric pain in Anatolia. Ferula L. is represented by 23 taxa in Turkey, 13 of which are endemic species. F. huber-morathii Peşmen (FHM), an endemic plant, is popularly known as ''helizan, çağşır''. Aim of the study: This study aimed to isolate sesquiterpenoids from the roots of Ferula huber-morathii (FHM) and to confirm their aphrodisiac potential in male rats. Material and methods: In a preliminary experiment, the effects of aqueous (H 2 O) and chloroform (CHCl 3) extracts of FHM were tested for their potential aphrodisiac activities in male rats. Then, sesquiterpene derivatives were isolated from the active chloroform extract of FHM roots (FHM-R) and characterized (TLC, 1D, 2D NMR, HR-MS and CD). Moreover, some of the isolates with adequate quantities were evaluated for their possible aphrodisiac effects on male rats. Single doses (10 mg/kg BW) of sildenafil citrate (SC, positive control), gummosin, mogoltavidin, deacetylkellerin, ferukrin acetate with kellerin, elaeochytrin-A and ferutinin were administered orally by gavages to male Wistar albino rats. Mount latency (ML), mount frequency (MF), intromission latency (IL), intromission frequency (IF), ejaculation latency (EL) and postejaculatory interval (PEI) were studied. In addition, copulatory efficiency (CE) and intercopulatory efficiency (ICE) were calculated. Results: The preliminary experiment revealed that the chloroform extract was the main source of the active compounds as it showed the higher aphrodisiac activity while the aqueous extract was found to be inactive. Eleven sesquiterpene derivatives, viz. gummosin, mogoltavidin, farnesiferol A, deacetylkellerin, ferukrin acetate, kellerin, teuclatriol, feruhermonin C, ferutinin, elaeochytrin A and teferidin, were isolated from the FHM-CHCl 3 extract. Oral administration of deacetylkellerin, elaeochytrin-A and ferutinin significantly increased MF and IF. The ML and IL were significantly reduced, and ejaculation latencies were prolonged. Administration of these sesquiterpenoids also reduced the PEI. The present results revealed that ferutinin was the most effective aphrodisiac compound compared to other sesquiterpenoids. The results of 10 mg/kg of ferutinin are comparable to SC, the positive control. The results revealed that gummosin, mogoltavidin and ferukrin acetate with kellerin did not significantly alter the aphrodisiac parameters. Conclusions: This study has established that the CHCl 3 extract of FHM root contains sesquiterpene derivatives, especially coumarin ethers and benzoic esters. Findings of the present study demonstrate that the chloroform extract and some of the sesquiterpene derivatives significantly stimulates sexual behavior in male rats, thus suggesting that F. huber-morathii possesses an aphrodisiac activity.

Journal of The Saudi Pharmaceutical Society, Nov 1, 2019

A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been es... more A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone: water: formic acid, 10:6:2:2 (%, v/v/v/v)) on 20 Â 10 cm glass coated silica gel 60 F 254 plates and scanned at 254 nm for ascorbic acid and gallic acid. Ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice were identified by comparing their single spot at R f = 0.54 ± 0.02 and R f = 0.83 ± 0.01 respectively. The value of regression equation (r 2 ! 0.9992) revealed a good linear relationship between peak area and amount of ascorbic acid and gallic acid in the range of 100-800 ng/band. The method was validated for precision, accuracy, robustness LOD and LOQ. The method proposed can be useful for routine determination of ascorbic acid and gallic acid in various crude as well as herbal formulations as a quality control tool.

Pharmaceutical Biology, Dec 23, 2011

Context: Emex spinosa (L.) Campd. (Polygonaceae), Leptadenia pyrotechnica (Forsk.) Decne (Asclepi... more Context: Emex spinosa (L.) Campd. (Polygonaceae), Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae), Haloxylon salicornicum (Moq.) Bunge ex Bioss. (Chenopodiaceae) and Ochradenus baccatus Delile (Resedaceae) are used in folk medicine for treatment of male sexual disorders. Objective: To investigate the effects of E. spinosa, L. pyrotechnica, H. salicornicum and O. baccatus extracts on the reproductive system of male rats after prolonged period of treatment. Methods: Seventy-eight healthy adult male Wistar rats were divided into 13 groups (6 animals, each). The plant extracts (100, 200 and 400 mg/kg) were given daily by gavage to different groups of rats for 65 days. The thirteenth group (control) received the vehicle only. Test and control rats were mated with estrus female rats on days 30, 45 and 60 of treatment. Body and relative reproductive organ weights, and sperm parameters were recorded. Results: Animals treated with the ethanol extracts of E. spinosa and L. pyrotechnica showed significant improvement of the relative weight of reproductive organs, sperm count, sperm motility and total sperm abnormality. The mean sperm count for E. spinosa group (400 mg/kg) was 233.7 ± 4.50 × 10 6 /mL, for L. pyrotechnica (200 and 400 mg/kg) groups were 237.0 ± 5.22 × 10 6 /mL and 240.3 ± 4.64 × 10 6 /mL, respectively and that of the control group was 218.1 ± 4.28 × 10 6 /mL. The sperm motility of the control group was 77.5 ± 2.12, those of E. spinosa (400 mg/kg) group was 87.3 ± 3.50% and those of L. pyrotechnica (200 and 400 mg/kg) groups were 86.0 ± 3.11 and 89.7 ± 2.90%, respectively. Ethanol extracts of E. spinosa (400 mg/kg) and L. pyrotechnica (200 and 400 mg/kg) significantly elevate the serum levels of testosterone (5.30 ± 0.15, 5.32 ± 0.20 and 5.66 ± 0.19 ng/mL, respectively vs 4.64 ± 0.16 ng/mL) and luteinizing hormone (0.69 ± 0.03, 0.70 ± 0.03 and 0.74 ± 0.03 mIU/mL, respectively vs 0.59 ± 0.02 mIU/mL). On the other hand, no alterations were observed in body and relative organ weights, sperm numbers as well as sperm morphology of the male rats after the exposure to the H. salicornicum and O. baccatus extracts for 65 days. Conclusions: E. spinosa and L. pyrotechnica extracts appear to possess fertility improvement activity in male rats due to their testosterone increasing property. Moreover, the results suggest the absence of male reproductive toxicity of the H. salicornicum and O. baccatus extracts at tested doses.

Planta Medica, Jul 14, 2014

Pharmacognosy Journal, Apr 11, 2014

The anti-inflammatory and hepatoprotective activitiesof the methanolic extract of Anthemis scrobi... more The anti-inflammatory and hepatoprotective activitiesof the methanolic extract of Anthemis scrobicularis(ANS) herbs were evaluated in rats against carrageenan induced inflammation and carbon tetrachloride (CCl 4)induced hepatic injury. To evaluate the anti-inflammatory effects of ANS, twenty male rats were divided into four equal groups. Injection of 100 μl carrageenan in normal saline into the subplantar region of the hind paw of rats clearly induced paw edema. The volume of paw edema was attenuated following oral administration of ANS. For hepatoprotective effects, twenty five rats were equally divided into five groups.The hepatotoxicity, induced by a single dose of CCl 4 , produced significant (p<0.001) increase of the levels of serumtransaminase, phosphatase, bilirubin and a decrease in proteins were also noticed. The oxidative stress marker such as malondialdehyde (MDA)was increased and nonprotein sulfhydryl (NP-SH) was decreased in the hepatotoxic tissues. Pre-medication of CCl 4-intoxicated rats with ANS at the doses 250 and 500 mg/kg reversed the abnormal liver diagnostic stricture. The results showed that ANS is toxicologically safe when orally administered and possess highly significant anti-inflammatory and hepatoprotective activities and the potentials usefulness of Anthemis scrobicularis in hepatic and inflammatory disease.

Chemistry & Biodiversity, Jul 21, 2022

Tropical Journal of Pharmaceutical Research, Feb 15, 2018

Purpose: To explore the anti-inflammatory and hepatoprotective potentials of Silene villosa Caryo... more Purpose: To explore the anti-inflammatory and hepatoprotective potentials of Silene villosa Caryophyllaceae methanol extract in rats. Methods: Toxicity of S. villosa extract was evaluated in rats. Inflammation was induced in rats by injection of 0.1 mL carrageenan (1 %) in the left hind paws. Carbon tetrachloride (CCl4) was used to induce liver damage. Five groups of rat were used. The 1st (normal control) and 2nd (hepatotoxic) groups received the vehicle. The 3rd, 4th, and 5th groups received silymarin, 250 and 500 mg/kg of S. villosa extract, respectively, for 7 days. Liver injury was induced on the 7th day by intraperitoneal administration of 1 mL/kg of CCl4 to rats in groups 2-5. Results: The results showed that S. villosa is safe. It significantly reduced carrageenan-induced edema compared to normal (p < 0.01) and standard (p < 0.01) groups. The extract protected (p < 0.01) rats against the deleterious effect of CCl4. It decreased (p < 0.01) the elevated serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transferase (γ-GT) and alkaline phosphatase (ALP) as well as elevated serum levels of bilirubin (BRN), compared to CCl4 control rats. Reduced activities of the antioxidant enzymes were significantly increased (p < 0.01) in rat liver, compared with CCl4 control group. The results were confirmed by histological findings in rat liver as the extract reduced necrosis and hydropic degeneration of hepatic tissue compared to CCl4 control group. Conclusion: The results suggest that S. villosa possesses anti-inflammatory and hepatoprotective activities in rats, and therefore, has therapeutic potentials in humans.

Jpc – Journal Of Planar Chromatography – Modern Tlc, Feb 1, 2020

The proposed research study was undertaken to establish a simple, rapid and sensitive high-perfor... more The proposed research study was undertaken to establish a simple, rapid and sensitive high-performance thin-layer chromatography (HPTLC) method for the quantification of trigonelline (TGN) in methanolic, aqueous and hydroalcoholic extracts of different brands of coffee samples with different colours due to roasting time. The proposed HPTLC methodology was carried out using glass coated silica gel 60 F 254 plates. The mobile phase used was quaternary mixture of ethyl acetate:methanol:water:formic acid 5.4:2.3:1.5:0.8 (%, v / v ) which furnished a compact spot of TGN at Rf 0 . 24 ± 0 . 01. The developed plate was scanned and quantified densitometrically at 267 nm. Linear regression analysis presented a good relationship between peak area and amount of TGN in the range of 100–700 ng/band. The method was validated as per the International Conference on Harmonization (ICH) recommendations. The maximum amount of TGN was recorded in roasted Arabic coffee among different brands studied. The amounts of TGN in methanolic, aqueous and hydroalcoholic extract of roasted Arabic coffee were obtained as 0.74, 0.80 and 0.79% w / w , respectively. The proposed analytical methodology will be useful to enumerate the therapeutic dose of TGN in different herbal products.

Journal of pharmaceutical research international, Dec 20, 2019

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condime... more Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants. Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline. Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a

Evidence-based Complementary and Alternative Medicine, Apr 20, 2023

Background. Quercetin hastraditionally been used in various oxidative and urinary tract dysfuncti... more Background. Quercetin hastraditionally been used in various oxidative and urinary tract dysfunctions. Tecurrent project is consequently set to evaluate the defensive efcacy ofQuercetin against potassium bromate (KBrO3) induced testiculartissue oxidative dysfunctions through biochemical, hormonal, and genotoxicmarkers.Methods. To observe theprotective efcacy of Quercetin against urinogenital oxidative dysfunction inrats, thirty six albino male rats were divided into six groups. Protectiveefcacies of Quercetin were checked on reproductive hormonal levels,antioxidant enzyme activities, lipids peroxidation (LP), and DNA damages. Results. Potassium bromate exposure in experimentalanimals caused a reduction in the activities of antioxidant enzymes and disturbedhormonal secretions while enhancing the peroxidation of lipids andfragmentations of DNA. Cotreatment of Quercetin considerably (P<0.01)reversed these abnormalities with admiration to levels of hormones, antioxidantenzymes activities, and peroxidations of lipids secure to those seen inuntreated rats. (P < 0.01)Conclusion. Te fndings of the current project revealedthat various doses of Quercetin are able to keep the testicular organ fromabnormal free radical dysfunctions. Tese improvements might be due to theantioxidant ability of polyphenolic bioactive constituent, i.e., Quercetin.

Applied sciences, Feb 25, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of The Saudi Pharmaceutical Society, Dec 1, 2019

The aim of this study is the development of validated HPTLC method for the quantification of vite... more The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was developed using ethyl acetate:methanol:water:formic acid 30:4:2:1(%, v/v/v/v) on 20 Â 10 cm glass coated silica gel 60 F 254 plates and the developed plate was scanned and quantified densitometrically at k = 340 nm. Linear regression analysis revealed a good linear relationship between peak area and amount of vitexin in the range of 100-700 ng/spot. The amount of vitexin in nine commercial herbal formulations was successfully quantified by the developed HPTLC method. The developed and validated high performance thin layer chromatographic method offers a new sensitive and reliable tool for quantification of vitexinin in various herbal formulations containing Passiflora foetida.

Pharmaceuticals, Aug 31, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

International Journal of Pharmacology, Sep 15, 2018

Planta Medica, Jul 14, 2014

Gels, Feb 24, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of Cosmetic Dermatology, Jan 6, 2023

Healthcare, Sep 13, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY