hayrunnisa nadaroglu - Independent Researcher (original) (raw)

Papers by hayrunnisa nadaroglu

Annual Research & Review in Biology, 2014

Removal of acid red 37 from aqueous solutions has been studied using red mud waste material after... more Removal of acid red 37 from aqueous solutions has been studied using red mud waste material after its modification with laccase from Russulaceae (Lactarius volemus). Laccase was purified by using saturated precipitate (NH 4) 2 SO 4 , diethylaminoethyl celulose (DEAEcellulose) and immobilized on red mud. The removal of acid red 37 by the laccasemodified red mud has been demonstrated in order to explore its potential use as low-cost adsorbent. The adsorption kinetics of acid red 37 dye on the laccase-modified red mud with respect to pH, contact time, temperature and adsorbent dose were investigated. The optimum results were obtained at pH 4, contact time of 60 min and temperature of 30ºC. The Freundlich equation was found to have the highest value of R 2 compared with the Langmuir model. Thermodynamic parameters including the Gibbs free energy, enthalpy and entropy changes indicated that the adsorption of acid red 37 onto laccase-modified red mud was feasible, spontaneous and endothermic.

Journal of the Hellenic Veterinary Medical Society, 2022

In this study, Enterococcus faecalis proteolytic strains which have the potential to degradation ... more In this study, Enterococcus faecalis proteolytic strains which have the potential to degradation of bovine milk proteins were isolated from Turkish White Pickled Cheeses and milk samples. E. faecalis strains were found to have strong caseinolytic activity. The extracellular protease enzymes produced by E. faecalis strains from 60 different samples were analyzed in the pattern of bands on a stained SDS-PAGE gel. The highest proteolytic activity of E. faecalis isolates were determined at pH 7.0 and 40 ℃ for 24 h. In addition, antimicrobial resistance and the presence of selected virulence genes of isolates were investigated for microbiological safety. These findings further emphasize that the E. faecalis isolates can be effective in the degradation of bovine milk proteins.

Molecules, 2020

Pectinases are an important class of enzymes distributed in many higher plants and microorganisms... more Pectinases are an important class of enzymes distributed in many higher plants and microorganisms. One of these enzymes is pectin lyase which has an important role in industrial applications such as clarification of fruit juices. Pectin lyase was purified with 73% yield from Pseudomonas putida bacteria and was 220.7-fold using three phase precipitation technique. Molecular weight of purified pectin lyase was determined as 32.88 kDa with SDS-polyacrylamide gel electrophoresis. The pectin lyase was immobilized covalently via the L-glutaraldehyde spacer to the cellulosic structures of lily flowers (Lilium candidum L.). The immobilized enzyme was then magnetized by modifying with γ-Fe3O4 nanoparticles and determined the most appropriate immobilization conditions as pH 6 and 30 °C. Purified pectin lyase was connected to magnetized support material after 60 min at the rate of 86.4%. The optimum pH and temperatures for the free and immobilized pectin lyase was found to be 6.0 and 40 °C. pH...

Tumor marker, a substance sometimes found in the blood, other body fluids, or tissues. A high lev... more Tumor marker, a substance sometimes found in the blood, other body fluids, or tissues. A high level of tumor marker may mean that a certain type of cancer is in the body. Examples of tumor markers include CA 125 (ovarian cancer), CA 15-3 (breast cancer), CEA (ovarian, lung, breast, pancreas, and gastrointestinal tract cancers), and PSA (prostate cancer). Tumor marker also called biomarker [1,2]. Measurements of tumor marker levels can be useful when used along with x-rays or other tests in the detection and diagnosis of some types of cancer[3].

Determination of the Synthesis and Activity of CoO Nanoparticles Showing Antimicrobial Properties by the Green Synthesis Method

Unique sizes and high bioavailability of metal oxide nanoparticles (MONP) allow for interacting d... more Unique sizes and high bioavailability of metal oxide nanoparticles (MONP) allow for interacting directly with infectious agents, toxins, different chemical compounds and cell structures (proteins, lipids, nucleic acids). In some studies, it has been found that nanoparticles have the potential to be used in different forms, due to their less toxicity than microscopic materials. Todays, nanoparticle synthesis, which is a large research topic, has gained importance. For this purpose, physical, chemical and biological methods are used. Cobalt oxide (CoO) nanoparticles(NPs) have been used in many fields such as gas sensor, battery production and antimicrobial studies in nano size and has attracted much attention because of its potential applications.

Polish Journal of Environmental Studies, 2013

Cadmium is a toxic metal widely used in industry and it enters the environment from electroplatin... more Cadmium is a toxic metal widely used in industry and it enters the environment from electroplating, smelting, alloy manufacturing, pigments, plastics, cadmium-nickel batteries, fertilizers, pesticides, mining, pigments and dyes, textile operations, and refining industries. Considering the harmful effects of cadmium, it is necessary to remove cadmium from liquid wastes at least to the limit accepted by regulations. The present study describes removal of cadmium from aqueous solutions using bacteria-modified red mud waste material. Batch adsorption experiments have been performed as a function of pH, contact time, temperature, and adsorbent dosage. The optimum results were obtained at pH 4.0, contact time of 60 min, temperature of 30oC, and an adsorbent dose of 1 mg/mL. The adsorption data was correlated with Langmuir and Freundlich adsorption models. The maximum adsorption capacity obtained from Langmuir adsorption model was 83.034 mg/g. The kinetic processes of cadmium adsorption on...

Investigation of Antimicrobial and Genotoxic Effects of Fe2O3, NiO and CoO NPs Synthesized by Green Synthesis

It is seen that metal nanoparticles are used in many areas due to their antimicrobial effects. Fo... more It is seen that metal nanoparticles are used in many areas due to their antimicrobial effects. For this reason, our study focused on the production of -Fe2O3, NiO and CoO NPs of golden nanoparticles, which are easily obtained with the use of Erzincan grape extract, safe to use, environmentally friendly and cost-effective. Metal ions synthesized by the green synthesis method were characterized using the Scanning Electron Microscope (SEM) analysis. From the SEM diagrams of the synthesized nanoparticles, it was determined that the nanoparticles were approximately 5 to 65 nm in size. Both antimicrobial, genotoxicity and cytotoxicity effects were investigated to determine the rates at which nanoparticles can be used as biosafe. Synthesized -Fe2O3, NiO and CoO NPs showed excellent antibacterial properties on pathogen bacteria againist human. In addition, it was determined that -Fe2O3, NiO and CoO M-NPs showed genotoxic properties in parallel with increasing concentrations. This study, ...

The effects of metals on the ecological system are very important, and their presence on certain ... more The effects of metals on the ecological system are very important, and their presence on certain limit values has negative effects on the living things. In particular, it has been determined that structural and functional changes are present in plants exposed to metals at high rates. The accumulation of heavy metals in the environment is a serious problem. In this study, the effect of heavy metals such as Co2+, Ni2+, Cu2+ and As3+ on the plant growth of the seeds of Ispir bean (Phaseolus vulgaris L.), a species peculiar to the Ispir region of Erzurum and well known by public, was investigated. As a result, it was observed that when the seeds were exposed to heavy metals, the plant showed an unhealthy growth and was adversely affected.

Iron Ores and Iron Oxide Materials, Jul 11, 2018

In this study, loaded Luffa sponge membrane forms were modified with ZnO, Fe 3 O 4 , ZnO/ Fe 3 O ... more In this study, loaded Luffa sponge membrane forms were modified with ZnO, Fe 3 O 4 , ZnO/ Fe 3 O 4 nanoparticles (NPs) to remove of Direct Blue 15 (DB15), which is a carcinogenic azo dye in aqueous solution. ZnO and Fe 3 O 4 NPs were synthesized using purified peroxidase enzymes from Euphorbia amygdaloides using green synthesis method. Adsorption of DB15 azo dyes was separately studied with membrane forms (LS-pure, LS-ZnO, LS-Fe 3 O 4 , and LS-ZnO/Fe 3 O 4). Optimum contact time, optimum pH, optimum temperature, optimum dye concentration, and optimum LS amount were found as 45 min, pH 8.0, 20°C, 200 mg/L, and 0.025 g in line with the optimization studies, respectively. The obtained membrane forms were characterized using SEM, FT-IR, and XRD techniques. According to obtained results, NPs loaded LS membrane forms are promising in removal of DB15 from textile wastewater contaminated water.

Archives of Biological Sciences, 2018

In this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI 4)-induced hepat... more In this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI 4)-induced hepatic injury in the rat was investigated. A hepatotoxic rat model was developed by the injection of CCI 4 dissolved in soybean oil (1 mL/kg/twice a week, intraperitoneal (i.p.) injection). Following the formation of hepatic injury, extracts of M. nigra at doses ranging from 150 to 300 mg/kg were administered to rats by i.p. injection for eight weeks. At the end of administration, rat livers were excised by dissection. The activities of liver enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) were detected in the serum, and the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were established in the liver. Histological changes and immunohistochemical localization of caspase-3 and 8-oxo-2'-deoxyguanosine (8-OhdG) were performed by hematoxylin-eosin (H&E) staining of liver sections and caspase-3 and 8-OhdG immunohistochemical staining. The results showed that the M. nigra extract prevented protein oxidation generated by CCI 4. The extracts demonstrated the ability to modulate the activity of SOD and GPx, and also prevented the CCI 4-induced increase in AST and GGT levels. These results indicate that M. nigra extracts provided significant protection against CCl 4-induced hepatic injury and might also present a novel approach for the treatment of some liver diseases.

Journal of the Institute of Brewing, 2017

There is increased interest in using facultative aerobic bacteria to produce bioethanol from wast... more There is increased interest in using facultative aerobic bacteria to produce bioethanol from waste materials, including complex hemicellulosic biomass. In this laboratory study, ethanol was produced by different facultative aerobic bacteria (Lactobacillus plantarum M24, Lactobacillus sakei LB49, Weissella viridescens LB37 and Pediococcus acidilactici M17) from diverse waste materials (corn, corncob, paper and pine cones). Of these, corn was found to be the best substrate for bioethanol production with L. plantarum M24 the most efficient bacterium for most of the selected waste materials.

Asian Journal of Chemistry, 2014

The presence of heavy metals in the aquatic ecosystem causes harmful effects to living organisms ... more The presence of heavy metals in the aquatic ecosystem causes harmful effects to living organisms and causes environmental hazards 1. Inorganic effluents contaminate wastewater with toxic metals such as Ni, Cr, Cd, Hg, Pb and Zn 2-4. These heavy metals are extremely toxic, non-biodegradable and accumulate in the food chain, even in relatively minute quantities. Thus, industries such as electroplating, aerospace and metal finishing are among the most hazardous from a wastewater viewpoint 5-6. For the past several years, high levels of heavy metals in drinking water and foods have been associated with several acute and chronic illnesses in humans throughout the world 7,8. Water pollution is the contamination of water bodies such as lakes, rivers, oceans and groundwater caused by human activities, which can be harmful to organisms and plants, present in water bodies. The presence of heavy metals in streams, lakes and groundwater reservoirs has been responsible for several health problems with plants, animals and human beings 9. Heavy metal contamination exists in aqueous waste stream from many industries such as metal plating, mining, tanneries, painting, car radiator manufacturing, as well as agricultural sources where fertilizers and fungicidal spray are intensively used 10-12. Nickel ion is one such heavy metal frequently encountered in raw wastewater streams from industries, such as non-ferrous

Green Processing and Synthesis, 2017

Nanotechnology is a growing area of research. For example, gold nanoparticles have a wide variety... more Nanotechnology is a growing area of research. For example, gold nanoparticles have a wide variety of applications, including optical, electronic, and medical; they are also used as catalysts in biosensor applications and can be used for scanning, carrier, heat source, and sensors. Therefore, nanoparticle synthesis is of great importance to the country’s economy and to scientific development. Nanoparticle synthesis involves using chemical methods, physical methods, or both chemical and physical methods. The synthesis is performed at high pressures or high temperatures and severe conditions, both of which have a high cost in terms of energy. In our investigation, we used a green synthesis method, which used quail eggs that required more moderate conditions and less energy. The yolk from quail eggs has a high protein and vitamin content. Using quail egg yolks, the reaction conditions were optimized in terms of pH, temperature, and concentration. The morphological properties of the obta...

Archives of Environmental Protection, 2016

The objective of this study was to investigate the possibility of using natural and bacteria-modi... more The objective of this study was to investigate the possibility of using natural and bacteria-modified Erzurum clayey soil with Methylobacterium extorquens as an alternative to high cost commercial adsorbent materials for the removal of copper from aqueous solution. The copper concentrations in the samples of the polluted river water and CuCl

Desalination and Water Treatment, 2015

CyTA - Journal of Food, 2015

Phytase enzyme was purified from the Pinar melkior (Lactarius piperatus) mushroom using ammonium ... more Phytase enzyme was purified from the Pinar melkior (Lactarius piperatus) mushroom using ammonium sulfate precipitation and DEAEsephadex ion exchange chromatography techniques. First, the purified phytase enzyme was covalently bound to the surface of magnetite-CTS NPs in yield of 87%. Then, optimum pHs of the free and immobilized enzymes were determined as 5.0 and 4.0, respectively. Optimum temperature of free and immobilized enzymes was found as 60°C. Also, the effects of some metal ions on activity of free and immobilized phytase enzymes were investigated. Also, research was undertaken as to whether the purified free and immobilized phytase enzyme could hydrolyze the phytic acid in many cereal products or not. And, it was discovered that the immobilized phytase enzyme hydrolyzed the phytic acid at the highest rate (75.02% rate) in wheat. From the findings obtained, that immobilized enzyme was quite resistant to temperature, pH and metal ions.

Clay Minerals, 2015

In this study, a clinoptilolite modified with apolaccase was used to adsorb Reactive Black 5 (RB5... more In this study, a clinoptilolite modified with apolaccase was used to adsorb Reactive Black 5 (RB5) dye from aqueous solution using the batch procedure. The influences of pH, contact time, temperature and absorbent dosage on the adsorption were investigated. The optimum adsorption was obtained at pH = 6, contact time = 60 min, temperature = 25ºC and adsorbent dosages of 1.62 and 1.59 mg/50 mL per gram of clinoptilolite and of apolaccase-modified clinoptilolite (LMC), respectively). The adsorption experimental data fitted both the Langmuir and Freundlich isotherm models well. In addition, pseudo-first-order and pseudo-second-order kinetics were used to study the kinetics of RB5 dye adsorption onto natural clinoptilolite and LMC. Adsorption appears to follow pseudo-second-order kinetics with a high correlation coefficient. Thermodynamic parameters such as changes in the free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) of adsorption were calculated. The thermodynamic parameters indic...

Indian journal of biochemistry & biophysics, 2007

Four isoenzymes of carbonic anhydrase (CA) were purified from Elephas Irogontherii (steppe elepha... more Four isoenzymes of carbonic anhydrase (CA) were purified from Elephas Irogontherii (steppe elephant) bone (approx 0.3-0.5 million years old) from different locations (outer peripheral, cytosolic, inner peripheral and integral) using Sepharose 4B-L-tyrosine sulphanilamide affinity chromatography and their kinetics properties were investigated and compared with known CA isoenzymes. The purification degree of CAs was monitored by SDS-PAGE. Purification fold for outer peripheral, inner peripheral, cytosolic and integral CA was 395.6, 652.8, 1091 and 429.3 and the molecular mass (as determined by gel filtration chromatography) was 37, 36, 35, and 39 kDa, respectively. The optimal temperature for isozymes was 10-20, 30, 30 and 60 degrees C and optimal pH- was between 7.5-11, 7.5-10, 7.5-10 and 7.5 respectively. K(m) values (at optimum pH and 20 degrees C) for p-nitrophenyl acetate as substrate were 4.83, 6.80, 4.525 and 3.86 mM and the Vmax values for the same substrate were 0.00097, 0.01...

Drug Metabolism Letters, 2014

In this study, serum samples from 50 patients with the diagnosis of Behcet's disease and 20 healt... more In this study, serum samples from 50 patients with the diagnosis of Behcet's disease and 20 healthy volunteers were analyzed. The study consists of three parts. In the first part, paraoxonase (PON) activities were determined in the serum samples of 50 patients with Behcet's disease and 20 healthy people. In the second part, equal volumes of serum samples from 50 patients were pooled and PON enzymes were purified by using Sepharose-4B-L-tyrosine tyrosine-1-naphtylamine affinity column. Optimum temperature, optimum pH, Vmax and Km values of the pure enzymes were determined. The same purification procedure was also performed in the serum samples of 20 healthy people. Electrophoretic mobility was observed (via SDS-PAGE) in the PON enzymes that were purified from the serum samples of patients with Behcet's disease and healthy people. In the third part, in vitro effects of drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of Behcet's disease were tested on the PON enzymes of the patients with Behcet's disease and control group. IC 50 values and K i constant values were measured and inhibition types were determined for the drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of the Behcet's disease and demonstrate in vitro inhibition effects.

International Journal of the Physical Sciences, 2012

This study on the removal of cobalt ions from aqueous solutions by red mud was conducted in batch... more This study on the removal of cobalt ions from aqueous solutions by red mud was conducted in batch condition. Its objective was to investigate the possibility of using red mud waste material as an alternative to high cost commercial adsorbent materials for the removal of cobalt from aqueous solution. In this study, batch experiments were carried out for the adsorption of cobalt onto red mud industry waste. The red mud waste material was successfully used for the removal of cobalt ion from aqueous solution and the technique appears industrially applicable and viable. The potential of economically cheap waste materials like red mud was assessed for cobalt adsorption from aqueous solutions. The operating variables studied were contact time, initial solution pH, initial metal concentration, temperature and adsorbent dosage. The experimental investigation results show that activated red mud has a high level of adsorption capacity for cobalt ion. The adsorption data was correlated with the Langmuir and Freundlich isotherm models. It was found out that the Langmuir and Freundlich isotherms fitted well to the data. Consequently, it was concluded that the red mud can be successfully used for the removal of the cobalt ions from the aqueous solutions with heavy metals.