hooshang khazan - Academia.edu (original) (raw)

Papers by hooshang khazan

Background: Fascioliasis in livestock and humans, caused by Fasciola hepatica and Fasciola gigant... more Background: Fascioliasis in livestock and humans, caused by Fasciola hepatica and Fasciola gigantica, has socioeconomic importance worldwide. Iran is one of the important endemic foci for fascioliasis. Molecular approaches can be precise and reliable for the identi cation and characterization of Fasciola spp. The aims of this study were molecular identi cation of Fasciola hepatica isolates in Golestan province, north of Iran, and then comparative analysis of results using GenBank sequences. Material and Method: Fasciola ukes were isolated from the livers of infected livestock. DNA was extracted from samples using the phenol-chloroform technique. RFLP-PCR using TasI restriction enzyme was performed along with morphometric evaluations for the detection of F. hepatica species. PCR was performed to amplify partial fragments of the internal transcribed spacer 1 (ITS1), cytochrome c oxidase sub.1 (CO1), and NADH dehydrogenase sub.1 (ND1) genes for samples that were con rmed as F. hepatica. Twenty-eight PCR products were sequenced. The phylogenetic tree with MEGA 7 software was drawn for all three genes. Results: Out of 271 ukes collected from sheep and cattle, 126 con rmed as F. hepatica by the PCR-RFLP method. Results based on PCR-RFLP analysis were con rmed by sequence analysis. Only one haplotype for the CO1 gene and four different haplotypes for the ND1 gene were identi ed. Seven sequences of each gene registered on GenBank and accession numbers were received. Conclusions: This study showed that F. hepatica is widely distributed among livestock in Golestan province. It was also found that genetic diversity among the ND1 region in isolates of our study was considerably higher than the CO1 sequence region.

Clinical Epidemiology and Global Health, Mar 1, 2020

Background: Intestinal parasitic infections (IPIs) are confirmed as major public health problems,... more Background: Intestinal parasitic infections (IPIs) are confirmed as major public health problems, worldwide. Northern Iran is highly endemic area for such infections. This research aimed to study on the prevalence of IPIs among people referred to the health care centers in Mazandaran Province, Northern Iran. Methods: In this descriptive cross-sectional survey, 984 stool samples were collected during 2015-2016. Fresh stool samples were collected from all individuals and examined by direct slide smear, formalin-ether concentration, trichrome and modified Ziehl-Neelsen staining techniques. The results were analyzed using SPSS version 16 and Chi-square test. Results: Out of 984 participants, the overall prevalence of IPIs was 12.1% (95 CI, 11.4-12.7; 119/984). The prevalence of protozoa and helminthes infections were 7.9% (95 CI, 7.4-8.3; 109/984) and 5.3% (95 CI, 4.9%-5.5%; 69/984), respectively. The most prevalent protozoan IPs were include Giardia lamblia (3.3%), Entamoeba coli (2%), Blastocystis hominis (1.3%), Entamoeba histolytica/dispar (1.1%), Entamoeba hartmanni (1.1%) and Cryptosporidium spp. (0.6%). Also major helminthic infections were include Trichostrongylus spp. (2.6%), Hookworms (1.7%), Strongyloides stercoralis (1.1%), Hymenolepis nana (0.6%) and Dicrocoelium dendriticum (0.6%). The results of the univariate analysis revealed that residence (rural being [P = 0.017]) and occupation (farmers [0.033]) were statistically significant risk factor for IPIs. Conclusion: In conclusion, we have found, despite persistent improvement in sanitary conditions over the recent years in northern part of Iran, IPIs are still among the main public health problems in this area. Moreover, helminths infection in our study were considerable. Thus, effective control programs are essential to reduce the incidence of IPIs.

Iranian Journal of Public Health, 1996

Journal of Parasitology Research, 2015

This study reports data on the prevalence, morphology, and morphometry of the nematode Cheilospir... more This study reports data on the prevalence, morphology, and morphometry of the nematode Cheilospirura hamulosa on the basis of light and stereoscopic microscopy and also camera lucida. Specimens were recovered after necropsies of 100 partridges (Alectoris chukar) from Taleqan County in Alborz Province, Iran. The prevalence of C. hamulosa in partridges was of 30% with a mean intensity of 3.9 and range of infection of 1-12. The mean length and width of females were 17.5 ± 2.14 and 0.39 ± 0.04 mm, while those of males were 12.2 ± 0.67 and 0.3 ± 0.06 mm, respectively. The characteristic digitiform tail was observed in females, and the unequal spicules, caudal alae, and ten pairs of caudal papillae were seen in males. The taxonomic characteristic longitudinal cordons and muscular and glandular oesophagus were observed in both sexes. Ratio between cordons and body length in males and females was 1 : 1.33 and 1 : 1.68, respectively. Ratio between long and short spicules in males was 1 : 2.3. The average size of embryonated eggs was 51.25 × 29.5 m. In the present study, C. hamulosa (Nematoda: Acuarioidea) is recorded for the first time from partridges in Iran. Therefore, the morphological characters described in this study will be useful in the future diagnostic and taxonomic studies of Acuarioidea family.

Parasitology Research, Sep 5, 2012

This represents the first study to determine the genetic diversity of Blastocystis sp. among canc... more This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/ AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.

Parasitology Research, 2009

This represents the first study to determine the genetic diversity of Blastocystis sp. among canc... more This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/ AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.

Iranian Journal of Parasitology

Background: After the earthquake in 2017 a few new cases of visceral leishmaniasis (VL) were repo... more Background: After the earthquake in 2017 a few new cases of visceral leishmaniasis (VL) were reported from SarPol-e-Zahab district of Kermanshah Province, western part of Iran. This study was conducted to determine the seroprevalence in Kermanshah Province. Methods: This descriptive cross-sectional study was conducted on children up to 12 years of age from SarPol-e-Zahab County, Kermanshah Province, western part of Iran in 2021. For each individual, a questionnaire including age, sex, clinical features, history of the disease, and contact with canines as reservoir hosts of VL were completed, separately. To determine VL seroprevalence, blood samples were collected from the children and after centrifugation, the sera samples were separated and tested using Direct Agglutination Test (DAT) for detection of anti-L. infantum antibodies. Statistical analyses were performed using SPSS16. Results: Totally, 13 persons were seropositive; 7 samples with titer 1:800, 3 samples had 1:1600, 2 sa...

Journal of Ardabil University of Medical Sciences, 2004

Objective: Successful malaria elimination program calls for enough attention to parasite carriers... more Objective: Successful malaria elimination program calls for enough attention to parasite carriers, especially asymptomatic malaria, as well as early detection and treatment of clinical cases. Patients with asymptomatic malaria do not show any symptoms. thus, these patients play a critical role in the elimination program. The current investigation was conducted to evaluate the presence of asymptomatic malaria cases in Bashagard district, a highly malaria transmission area in Hormozgan Province, Iran. Methods: This Cross sectional study was carried out in Bashagard district, Hormozgan Province of Iran, in July 2009. A total of 500 samples were randomly collected from symptomless residents of Bashagard to evaluate Plasmodium infection rate by using microscopic, serologic and nested-PCR (using 18ssrRNA) techniques. Results: Regarding the microscopic and nested-PCR analysis, no Plasmodium infection was detected among the participants. In this study, 1% of the analyzed population (5/500) ...

مجله دانشگاه علوم پزشکی گرگان, 2017

Background and Objective: Identification of Fasciola species is important. Fascioliasis is one of... more Background and Objective: Identification of Fasciola species is important. Fascioliasis is one of the important diseases in animals and humans caused by genus Fasciola. This study was done to determine the identification of Fasciola species with RFLP-PCR in animal liver in Gorgan City, northern Iran. Methods: In this descriptive study, worms were obtained from the livers of infected sheep and cattle in Gorgan slaughterhouse in northern Iran. DNA of worms was extracted with phenol- chloroform method. Fragment of ITS-1 genome was amplified and TasI enzyme was utilized for amplified fragments then 8 samples were sequenced. Results: A total of 49 Fasciola worms were isolated from infected cattle and sheep. The PCR products of all specimens were affected by the TasI enzyme, and F.hepatica species showed two fragments and F.gigantica species indicated three fragments. The enzyme in F.hepatica species showed a fragment of 151 bp and a fragment of 312, but in the F.gigantica, three fragment...

Novelty in Biomedicine, 2017

Background:intestinal parasitic infection is one of the most prevalent health problems in develop... more Background:intestinal parasitic infection is one of the most prevalent health problems in developing countries. This study was conducted to determine the prevalence of intestinal parasitic infection and its correlation with socio-demographic parameters in Haji-abad, 2015. M aterialsandMethods: This cross-sectional descriptive study was conducted on 635 samples. After completing questionnaires, stool samples were assessed macroscopically, and microscopically using direct slide smear with saline and lugol, formalin-ether concentration, Ziehl-Neelsen staining to track Cryptosporidium species and Trichrome staining for the samples suspected to amoeba and other indeterminate cases. PCR using specific primers was conducted for Entamoebahistolytica/E. dispar suspected samples. The results were analyzed using SPSS ver.16 software. R es ults: Of total 635 samples, 198 cases (31.2%) were infected by at least one intestinal parasite. The most common parasites in this area were: Blastocystis sp...

Iranian Journal of Public Health, 2020

1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Med... more 1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Community Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran 4. Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 5. Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Novelty in Biomedicine, 2017

Background: Visceral leishmaniasis (VL) or kala-azar is a parasitic disease caused by the species... more Background: Visceral leishmaniasis (VL) or kala-azar is a parasitic disease caused by the species of Leishmania donovani complex. Mediterranean type of the disease is endemic in some parts of Iran and more than 95% of cases were reported in children up to 12 years of age. This study was performed to determine the seroprevalence of VL in the rural areas of the Dashti district from Bushehr province. M aterials and Methods: In this cross-sectional study, a randomized cluster sampling method was used for the collection of blood samples from children up to 12 years old from rural areas of Dashti district. Before sampling; a questionnaire was filled out for each case. All the collected blood samples were examined after the serum separating by Direct Agglutination Test (DAT) for detection of anti- Leishmania infantum antibodies. The cutoff titers of ≥1: 3200 with specific clinical features were supposed to be considered as VL. R es ults: Altogether, 24 out of 1221 (1.96%) blood samples sho...

Novelty in Biomedicine, 2017

Background: Visceral leishmaniasis (VL), caused by Leishmania infantum (L. infantum), is a life-t... more Background: Visceral leishmaniasis (VL), caused by Leishmania infantum (L. infantum), is a life-threatening vector-borne parasitic disease is distributed in some parts of the world. The disease is endemic in some parts of Iran. This study was aimed to determine the seroprevalence of VL among children and domestic dogs (as a reservoir of the parasite) in Dehloran, west of Iran. M aterials and Methods: This cross-sectional study was carried out in Dehloran County. The blood samples of 872 children up to 12 years old and 52 dogs were collected from 10 villages of Dehloran using randomly-clustered sampling method. Sera were separated from all peripheral blood samples and tested by direct agglutination test (DAT). Anti- Leishmania infantum antibodies at titers of ≥1:800 and ≥1:80 were considered as Leishmania infantum infection in human and dog, respectively. R es ults: In general, among 872 human samples, 1.03% of samples had anti- Leishmania antibody with 1:1600 titers and 1.26% had 1:...

Novelty in Biomedicine, 2018

Background: Toxocarosis is a zoonotic disease with worldwide distribution. Humans’ infection occu... more Background: Toxocarosis is a zoonotic disease with worldwide distribution. Humans’ infection occurred by incidental ingestion of eggs shed in feces of dogs or cats. Studies on general population are rare in Iran. In this cross-sectional study, we investigated seroprevalence, and risk factors associated with toxocariasis among the healthy individuals in Tehran, capital if Iran. M aterials and Methods: In total, 374 sera samples were investigated for the presence of anti-Toxocara IgG. We applied ELISA as screening test using available commercial kit. In addition, demographic data were obtained from participant’s questionnaires. Data analysis was performed using SPSS 16 . R es ults: The overall seroprevalence of toxocariasis was found 5.6% (21/374). Regarding the socio-demographic variables, age (P<0.001) and eating unwashed vegetables (P=0.049) were significantly associated to toxocariasis in univariate analysis. In the logistic regression analysis, only age (P<0.001) was identi...

Background: Leishmaniasis is an important parasitic disease in the world. We performed a seroepid... more Background: Leishmaniasis is an important parasitic disease in the world. We performed a seroepidemiological study of visceral leishmaniasis (VL) in Dehloran district, Ilam province to determine the seroprevalence of infection among humans and dogs using Direct Agglutination Test (DAT).Methods: In this descriptive analytic study, Sera were collected by a multi-stage randomized cluster sampling from 872 children ≤12 years and 52 dogs in 10 villages from four geographical areas in 2014. Direct Agglutination Test (DAT) was done for all samples after serum separation of blood in the leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. Results: In all, of 872 human, 9 samples were shown anti-Leishmania antibody with titers ≥ 1/1600 and 11 samples with titers ≥ 1/800. Of 52 dog samples, 11 dog samples with titers ≥ 1/320 as cut-off values of DAT and 13 with titers 1/80 and 1/160. Conclusion: None of collected human blood samples showed anti Lei...

Novelty in Biomedicine, 2017

Background: The genus Fasciola parasite causes fascioliasis infection. Fascioliasis is widespread... more Background: The genus Fasciola parasite causes fascioliasis infection. Fascioliasis is widespread all around the world and it is finding in abundance in the northern provinces of Iran. Cattle and sheep are the main hosts of the Fasciola parasite and intermediate hosts are lymnaeid snails such as Galba and Fossaria. Two main species of this genus are F. hepatica and F. gigantica. One of the most important methods of diagnosing this worm is morphological method. The aim of this study is to identify Fasciola through the morphological method in Golestan province. M aterials and Methods: Fasciola worms taken from infected livestock livers were washed three times with PBS and were stained with carmine alum. After staining using Valero و and Periago Periago methods, the worms were measured morphologically by و بcalibrated میکروسکوپ microscope, واستریومیکstereomicroscope, and True Chrome II camera. SPSS version 19 was used for analysis of the data. R es ults: A total of 45 livers from inf...

Food Control, 2018

A systematic review and meta-analysis on the global seroprevalence of Trichinella infection among... more A systematic review and meta-analysis on the global seroprevalence of Trichinella infection among wild boars

Food Control, 2018

Molecular identification of Trichinella spp. in wild boar, and serological survey of high-risk po... more Molecular identification of Trichinella spp. in wild boar, and serological survey of high-risk populations in Iran

Background: Fascioliasis in livestock and humans, caused by Fasciola hepatica and Fasciola gigant... more Background: Fascioliasis in livestock and humans, caused by Fasciola hepatica and Fasciola gigantica, has socioeconomic importance worldwide. Iran is one of the important endemic foci for fascioliasis. Molecular approaches can be precise and reliable for the identi cation and characterization of Fasciola spp. The aims of this study were molecular identi cation of Fasciola hepatica isolates in Golestan province, north of Iran, and then comparative analysis of results using GenBank sequences. Material and Method: Fasciola ukes were isolated from the livers of infected livestock. DNA was extracted from samples using the phenol-chloroform technique. RFLP-PCR using TasI restriction enzyme was performed along with morphometric evaluations for the detection of F. hepatica species. PCR was performed to amplify partial fragments of the internal transcribed spacer 1 (ITS1), cytochrome c oxidase sub.1 (CO1), and NADH dehydrogenase sub.1 (ND1) genes for samples that were con rmed as F. hepatica. Twenty-eight PCR products were sequenced. The phylogenetic tree with MEGA 7 software was drawn for all three genes. Results: Out of 271 ukes collected from sheep and cattle, 126 con rmed as F. hepatica by the PCR-RFLP method. Results based on PCR-RFLP analysis were con rmed by sequence analysis. Only one haplotype for the CO1 gene and four different haplotypes for the ND1 gene were identi ed. Seven sequences of each gene registered on GenBank and accession numbers were received. Conclusions: This study showed that F. hepatica is widely distributed among livestock in Golestan province. It was also found that genetic diversity among the ND1 region in isolates of our study was considerably higher than the CO1 sequence region.

Clinical Epidemiology and Global Health, Mar 1, 2020

Background: Intestinal parasitic infections (IPIs) are confirmed as major public health problems,... more Background: Intestinal parasitic infections (IPIs) are confirmed as major public health problems, worldwide. Northern Iran is highly endemic area for such infections. This research aimed to study on the prevalence of IPIs among people referred to the health care centers in Mazandaran Province, Northern Iran. Methods: In this descriptive cross-sectional survey, 984 stool samples were collected during 2015-2016. Fresh stool samples were collected from all individuals and examined by direct slide smear, formalin-ether concentration, trichrome and modified Ziehl-Neelsen staining techniques. The results were analyzed using SPSS version 16 and Chi-square test. Results: Out of 984 participants, the overall prevalence of IPIs was 12.1% (95 CI, 11.4-12.7; 119/984). The prevalence of protozoa and helminthes infections were 7.9% (95 CI, 7.4-8.3; 109/984) and 5.3% (95 CI, 4.9%-5.5%; 69/984), respectively. The most prevalent protozoan IPs were include Giardia lamblia (3.3%), Entamoeba coli (2%), Blastocystis hominis (1.3%), Entamoeba histolytica/dispar (1.1%), Entamoeba hartmanni (1.1%) and Cryptosporidium spp. (0.6%). Also major helminthic infections were include Trichostrongylus spp. (2.6%), Hookworms (1.7%), Strongyloides stercoralis (1.1%), Hymenolepis nana (0.6%) and Dicrocoelium dendriticum (0.6%). The results of the univariate analysis revealed that residence (rural being [P = 0.017]) and occupation (farmers [0.033]) were statistically significant risk factor for IPIs. Conclusion: In conclusion, we have found, despite persistent improvement in sanitary conditions over the recent years in northern part of Iran, IPIs are still among the main public health problems in this area. Moreover, helminths infection in our study were considerable. Thus, effective control programs are essential to reduce the incidence of IPIs.

Iranian Journal of Public Health, 1996

Journal of Parasitology Research, 2015

This study reports data on the prevalence, morphology, and morphometry of the nematode Cheilospir... more This study reports data on the prevalence, morphology, and morphometry of the nematode Cheilospirura hamulosa on the basis of light and stereoscopic microscopy and also camera lucida. Specimens were recovered after necropsies of 100 partridges (Alectoris chukar) from Taleqan County in Alborz Province, Iran. The prevalence of C. hamulosa in partridges was of 30% with a mean intensity of 3.9 and range of infection of 1-12. The mean length and width of females were 17.5 ± 2.14 and 0.39 ± 0.04 mm, while those of males were 12.2 ± 0.67 and 0.3 ± 0.06 mm, respectively. The characteristic digitiform tail was observed in females, and the unequal spicules, caudal alae, and ten pairs of caudal papillae were seen in males. The taxonomic characteristic longitudinal cordons and muscular and glandular oesophagus were observed in both sexes. Ratio between cordons and body length in males and females was 1 : 1.33 and 1 : 1.68, respectively. Ratio between long and short spicules in males was 1 : 2.3. The average size of embryonated eggs was 51.25 × 29.5 m. In the present study, C. hamulosa (Nematoda: Acuarioidea) is recorded for the first time from partridges in Iran. Therefore, the morphological characters described in this study will be useful in the future diagnostic and taxonomic studies of Acuarioidea family.

Parasitology Research, Sep 5, 2012

This represents the first study to determine the genetic diversity of Blastocystis sp. among canc... more This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/ AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.

Parasitology Research, 2009

This represents the first study to determine the genetic diversity of Blastocystis sp. among canc... more This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/ AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.

Iranian Journal of Parasitology

Background: After the earthquake in 2017 a few new cases of visceral leishmaniasis (VL) were repo... more Background: After the earthquake in 2017 a few new cases of visceral leishmaniasis (VL) were reported from SarPol-e-Zahab district of Kermanshah Province, western part of Iran. This study was conducted to determine the seroprevalence in Kermanshah Province. Methods: This descriptive cross-sectional study was conducted on children up to 12 years of age from SarPol-e-Zahab County, Kermanshah Province, western part of Iran in 2021. For each individual, a questionnaire including age, sex, clinical features, history of the disease, and contact with canines as reservoir hosts of VL were completed, separately. To determine VL seroprevalence, blood samples were collected from the children and after centrifugation, the sera samples were separated and tested using Direct Agglutination Test (DAT) for detection of anti-L. infantum antibodies. Statistical analyses were performed using SPSS16. Results: Totally, 13 persons were seropositive; 7 samples with titer 1:800, 3 samples had 1:1600, 2 sa...

Journal of Ardabil University of Medical Sciences, 2004

Objective: Successful malaria elimination program calls for enough attention to parasite carriers... more Objective: Successful malaria elimination program calls for enough attention to parasite carriers, especially asymptomatic malaria, as well as early detection and treatment of clinical cases. Patients with asymptomatic malaria do not show any symptoms. thus, these patients play a critical role in the elimination program. The current investigation was conducted to evaluate the presence of asymptomatic malaria cases in Bashagard district, a highly malaria transmission area in Hormozgan Province, Iran. Methods: This Cross sectional study was carried out in Bashagard district, Hormozgan Province of Iran, in July 2009. A total of 500 samples were randomly collected from symptomless residents of Bashagard to evaluate Plasmodium infection rate by using microscopic, serologic and nested-PCR (using 18ssrRNA) techniques. Results: Regarding the microscopic and nested-PCR analysis, no Plasmodium infection was detected among the participants. In this study, 1% of the analyzed population (5/500) ...

مجله دانشگاه علوم پزشکی گرگان, 2017

Background and Objective: Identification of Fasciola species is important. Fascioliasis is one of... more Background and Objective: Identification of Fasciola species is important. Fascioliasis is one of the important diseases in animals and humans caused by genus Fasciola. This study was done to determine the identification of Fasciola species with RFLP-PCR in animal liver in Gorgan City, northern Iran. Methods: In this descriptive study, worms were obtained from the livers of infected sheep and cattle in Gorgan slaughterhouse in northern Iran. DNA of worms was extracted with phenol- chloroform method. Fragment of ITS-1 genome was amplified and TasI enzyme was utilized for amplified fragments then 8 samples were sequenced. Results: A total of 49 Fasciola worms were isolated from infected cattle and sheep. The PCR products of all specimens were affected by the TasI enzyme, and F.hepatica species showed two fragments and F.gigantica species indicated three fragments. The enzyme in F.hepatica species showed a fragment of 151 bp and a fragment of 312, but in the F.gigantica, three fragment...

Novelty in Biomedicine, 2017

Background:intestinal parasitic infection is one of the most prevalent health problems in develop... more Background:intestinal parasitic infection is one of the most prevalent health problems in developing countries. This study was conducted to determine the prevalence of intestinal parasitic infection and its correlation with socio-demographic parameters in Haji-abad, 2015. M aterialsandMethods: This cross-sectional descriptive study was conducted on 635 samples. After completing questionnaires, stool samples were assessed macroscopically, and microscopically using direct slide smear with saline and lugol, formalin-ether concentration, Ziehl-Neelsen staining to track Cryptosporidium species and Trichrome staining for the samples suspected to amoeba and other indeterminate cases. PCR using specific primers was conducted for Entamoebahistolytica/E. dispar suspected samples. The results were analyzed using SPSS ver.16 software. R es ults: Of total 635 samples, 198 cases (31.2%) were infected by at least one intestinal parasite. The most common parasites in this area were: Blastocystis sp...

Iranian Journal of Public Health, 2020

1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Med... more 1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Community Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran 4. Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 5. Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

Novelty in Biomedicine, 2017

Background: Visceral leishmaniasis (VL) or kala-azar is a parasitic disease caused by the species... more Background: Visceral leishmaniasis (VL) or kala-azar is a parasitic disease caused by the species of Leishmania donovani complex. Mediterranean type of the disease is endemic in some parts of Iran and more than 95% of cases were reported in children up to 12 years of age. This study was performed to determine the seroprevalence of VL in the rural areas of the Dashti district from Bushehr province. M aterials and Methods: In this cross-sectional study, a randomized cluster sampling method was used for the collection of blood samples from children up to 12 years old from rural areas of Dashti district. Before sampling; a questionnaire was filled out for each case. All the collected blood samples were examined after the serum separating by Direct Agglutination Test (DAT) for detection of anti- Leishmania infantum antibodies. The cutoff titers of ≥1: 3200 with specific clinical features were supposed to be considered as VL. R es ults: Altogether, 24 out of 1221 (1.96%) blood samples sho...

Novelty in Biomedicine, 2017

Background: Visceral leishmaniasis (VL), caused by Leishmania infantum (L. infantum), is a life-t... more Background: Visceral leishmaniasis (VL), caused by Leishmania infantum (L. infantum), is a life-threatening vector-borne parasitic disease is distributed in some parts of the world. The disease is endemic in some parts of Iran. This study was aimed to determine the seroprevalence of VL among children and domestic dogs (as a reservoir of the parasite) in Dehloran, west of Iran. M aterials and Methods: This cross-sectional study was carried out in Dehloran County. The blood samples of 872 children up to 12 years old and 52 dogs were collected from 10 villages of Dehloran using randomly-clustered sampling method. Sera were separated from all peripheral blood samples and tested by direct agglutination test (DAT). Anti- Leishmania infantum antibodies at titers of ≥1:800 and ≥1:80 were considered as Leishmania infantum infection in human and dog, respectively. R es ults: In general, among 872 human samples, 1.03% of samples had anti- Leishmania antibody with 1:1600 titers and 1.26% had 1:...

Novelty in Biomedicine, 2018

Background: Toxocarosis is a zoonotic disease with worldwide distribution. Humans’ infection occu... more Background: Toxocarosis is a zoonotic disease with worldwide distribution. Humans’ infection occurred by incidental ingestion of eggs shed in feces of dogs or cats. Studies on general population are rare in Iran. In this cross-sectional study, we investigated seroprevalence, and risk factors associated with toxocariasis among the healthy individuals in Tehran, capital if Iran. M aterials and Methods: In total, 374 sera samples were investigated for the presence of anti-Toxocara IgG. We applied ELISA as screening test using available commercial kit. In addition, demographic data were obtained from participant’s questionnaires. Data analysis was performed using SPSS 16 . R es ults: The overall seroprevalence of toxocariasis was found 5.6% (21/374). Regarding the socio-demographic variables, age (P<0.001) and eating unwashed vegetables (P=0.049) were significantly associated to toxocariasis in univariate analysis. In the logistic regression analysis, only age (P<0.001) was identi...

Background: Leishmaniasis is an important parasitic disease in the world. We performed a seroepid... more Background: Leishmaniasis is an important parasitic disease in the world. We performed a seroepidemiological study of visceral leishmaniasis (VL) in Dehloran district, Ilam province to determine the seroprevalence of infection among humans and dogs using Direct Agglutination Test (DAT).Methods: In this descriptive analytic study, Sera were collected by a multi-stage randomized cluster sampling from 872 children ≤12 years and 52 dogs in 10 villages from four geographical areas in 2014. Direct Agglutination Test (DAT) was done for all samples after serum separation of blood in the leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. Results: In all, of 872 human, 9 samples were shown anti-Leishmania antibody with titers ≥ 1/1600 and 11 samples with titers ≥ 1/800. Of 52 dog samples, 11 dog samples with titers ≥ 1/320 as cut-off values of DAT and 13 with titers 1/80 and 1/160. Conclusion: None of collected human blood samples showed anti Lei...

Novelty in Biomedicine, 2017

Background: The genus Fasciola parasite causes fascioliasis infection. Fascioliasis is widespread... more Background: The genus Fasciola parasite causes fascioliasis infection. Fascioliasis is widespread all around the world and it is finding in abundance in the northern provinces of Iran. Cattle and sheep are the main hosts of the Fasciola parasite and intermediate hosts are lymnaeid snails such as Galba and Fossaria. Two main species of this genus are F. hepatica and F. gigantica. One of the most important methods of diagnosing this worm is morphological method. The aim of this study is to identify Fasciola through the morphological method in Golestan province. M aterials and Methods: Fasciola worms taken from infected livestock livers were washed three times with PBS and were stained with carmine alum. After staining using Valero و and Periago Periago methods, the worms were measured morphologically by و بcalibrated میکروسکوپ microscope, واستریومیکstereomicroscope, and True Chrome II camera. SPSS version 19 was used for analysis of the data. R es ults: A total of 45 livers from inf...

Food Control, 2018

A systematic review and meta-analysis on the global seroprevalence of Trichinella infection among... more A systematic review and meta-analysis on the global seroprevalence of Trichinella infection among wild boars

Food Control, 2018

Molecular identification of Trichinella spp. in wild boar, and serological survey of high-risk po... more Molecular identification of Trichinella spp. in wild boar, and serological survey of high-risk populations in Iran