hosam elshaer - Academia.edu (original) (raw)

Papers by hosam elshaer

Research paper thumbnail of Genetic diversity and relationship between Egyptian Vigna (Vigna spp. (L.) Walp.) taxa populations via Phenotypic and Molecular profiling

Vegetos/Vegetos - International journal of plant research, Mar 18, 2024

Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.

Research paper thumbnail of Responses of He-Ne Laser Irradiation on Agronomical Characters and Cytological of (Atropa Belladonna L.)

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

The goal of the present work was to investigate the influence of laser irradiation on in vitro an... more The goal of the present work was to investigate the influence of laser irradiation on in vitro and in vivo growth agro-morphological criteria, mitotic activities and the chromosomes on Atropa belladonna L. The influence of laser radiation on Atropa seeds was investigated using helium-neon (He-Ne) were exposed to levels of laser doses (0.0,10,15, 20, 25 and 30 J cm)-2 using 7 m W He-Ne laser (632.8 nm),with a power density of 4.02 m W cm) 2 .The most of morphological, floral parameters germination percentage, plant height (cm), no. of leaves per plant, no. of branches per plant, leaf Length, width of leaf, leaf area and root length were studied in, First, Second and third months. The results showed significant differences among the five doses of laser radiation on seed germination, seedling height, plant height, no. of Leaves per plant at 10,20 and 30 days. The best doses 25 Jcm-2 to have a stimulating effect on these traits. The cytogenetic effects of physical mutagens Laser beam (0.0,10,15, 20, 25 and 30 J cm)-2 were investigated on the root tip cells of Atropa belladonna L., Various types of chromosomal aberrations (multinucleate cells) were observed during the cytological analysis of the root tip cells of both treatments. On the other hand, the high doses caused significant reduction as comparing with the control for all studied traits.

Research paper thumbnail of Molecular Genetic Studies on (Ficus Palmata & Ficus Carica Subsp Rupestris.) Under the Effect of Gamma Radiation by Tissue Culture Techniques

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

Ficus spp. are used as medicine and are used to reducing the risk of cancer and heart disease. Th... more Ficus spp. are used as medicine and are used to reducing the risk of cancer and heart disease. The phytochemical screening of the Ficus palmata plant extracts showed the presence of alkaloids, tannins, flavonoids, terpenoids and cardiac glycosides. This investigation was undertaken by using tissue culture technique on Ficus palmata & Ficus carica subsp rupestris. Study the effect of gamma rays doses of 0.0, 20, 30 and 40 Gy on callus production and the secondary plant constituents. The highest callus fresh weightand callus induction were achieved by callus derived from (leaves & stem) explants, which were cultured on (MS) medium supplemented with different concentrations of 2,4-D at 2.0 (mg/l) and Kin at 0.2 (mg/l). Polymerase chain reaction (PCR) amplification using eight ISSR primers showed polymorphic patterns with different response to gamma radiation doses. found genetic differences at the molecular level using ISSR with gamma doses compared to control. The total number of polymorphic bands was 239 bands (99.58% polymorphism). Found that there was genetic stability between the mother plants and product tissue culture for plants (Ficus palmata & Ficus carica subsp rupestris). The chemical composition of Ficus palmata was studied by means of GC-MS analysis under the effect of gamma radiation. The best doses of gamma were 30 & 40 Gy, as they increased the secondary compounds of Ficus palmata.

Research paper thumbnail of Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

African Journal of Biological Sciences, May 13, 2022

Research paper thumbnail of Evaluation of genetic stability using SCoT markers and SDS-PAGE with gamma radiation on callus of (Atropa belladonna L.) and antioxidant activity

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

Due to the improvement of the mutant germplasm and its use in the creation of new mutant organism... more Due to the improvement of the mutant germplasm and its use in the creation of new mutant organisms, these mutations have been instrumental in meeting global food and nutritional protection challenges. Mutagenic therapies for plant breeding and for drug alkaloids development projects have contributed to a wide range of genetic variability. The primary commercial source of drug alkaloids such as atropine is Atropa belladonna. This paper focuses on the effect of gamma rays (γ-rays) on callus of Atropa belladonna at the molecular level and changes of biochemical metabolisms in callus. Atropa belladonna callus were irradiated with 0.0, 30, 60, 90, 120 and 150 Gy gamma rays after 6 weeks on induction media. Highly significant differences between effect of gamma rays (γ-rays) on callus of Atropa belladonna at 2,4-D 2 mg/L and Kin 0.5 mg/L respectively were recorded on callus fresh weight and callus dry weight. 120 Gy had higher callus fresh weight, dry weight, Dry matter content (%) than the control. The difference in ion levels were measured in control and callus irradiated. The results clearly decrease in ion levels with increase of gamma dose. On the other hand, the deferent of antioxidant activity were cleared between control and gamma ray's callus. The hist antioxidant activity dose 150 Gy which recorded 30.20 comparing with control 20.10. Polyacrylamide gel electrophoresis SDS-protein PAGE profiles were used. In irradiated plants, there were several new protein bands that could be used as markers for each dose. Using DNA-Start codon targeted (SCoT) polymorphism assay. There were two negative molecular markers which were found only in control as compared to irradiated callus, with molecular sizes 940 and 400 bp.

Research paper thumbnail of Detection aflatoxin production by local isolates of Aspergillus spp. and molecular characterization

Archives of Agriculture Sciences Journal, Dec 1, 2021

Five isolates of Aspergillus spp. were isolated from stored wheat cereal and peanuts (groundnuts)... more Five isolates of Aspergillus spp. were isolated from stored wheat cereal and peanuts (groundnuts) in the El-Monofiya governorate in Egypt. The isolates were identified using morphological and microscopic properties. The ITS (internally transcribed spacer) region was found in the location of 600 bp. Results compared with data in GenBank confirmed that three isolates belonged to Aspergillus flavus and two isolates belonged to Aspergillus parasiticus. Three strains of Aspergillus flavus (Af1, Af2 and Af3) and two isolates of Aspergillus parasiticus (Ap4 and Ap5) were tested to produce aflatoxin on Czapek's agar medium. To distinguish between aflatoxigenic and non-aflatoxigenic isolates, TLC (thin layer chromatography) and PCR (polymerase chain reaction) were used. The TLC technique was previously used to quantify aflatoxin (B1, B2, G1 and G2) of five strains of Aspergillus. Three strains Af1, Af2 and Af3 gave B2 (50 μg/kg). The other strains Ap4 and Ap5 gave B1 (50 μg/kg) and G1 (75 μg/kg) for Ap4 and G2 (100 μg/kg) for Ap5. The DNA from all isolates has been extracted and amplified by PCR to encode target genes for the development of toxins (omt-A). It was observed in 3 (60%) of isolates Af1, Af2 & Af3 that the genes O-methyltransferase gene (omt-A) 300 bp. In addition, four SCoT primers and six ISSR primers were used to genetically link five Aspergillus spp. All five strains were divided into two groups for ISSR and SCoT primers. ISSR and SCOT analysis yielded similar results with TLC and aflatoxin-specific genes.

Research paper thumbnail of Studies on Genetic Selection to Produce New Genotypes of Mung bean (Vigna Radiata L.) by Gamma Rays and SCoT Marker

Al-Azhar Journal of Agricultural Research

A power of genetic selection is for producing new pure strains of interest species. The interest ... more A power of genetic selection is for producing new pure strains of interest species. The interest genes that are specifically expressed must be identified to develop the criterion of selection. The short period of Mung bean development could adapt to a broad sense of different factors such as agro-and ecosystems , plant rotation, plantation, and variety of germplasm. Three field experiments were carried out under the condition of Egyptian soil between Branch of

Research paper thumbnail of 16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

Archives of Agriculture Sciences Journal, 2019

Cucumber is one of Egypt's most popular vegetables, although it is sensitive to a number of plant... more Cucumber is one of Egypt's most popular vegetables, although it is sensitive to a number of plant diseases. The current study aimed to develop superior Streptomyces bacteria for improved antagonistic to fungi using mutagenic agents. Two Streptomyces isolates were isolated from Egyptian soil and identified by biochemical methods according to Bergey's Manual and partial 16S rRNA sequencing. These isolates were phenotypically identified as Streptomycetes genera. Mutation experiment by using chemical ethyl methanesulfonate (EMS) mutation methods. Through mutagenesis by (EMS) out of 10 obtained mutants. The mutants of Streptomyces isolates gave height antagonism of cucumber plant fungi Fusarium, Pythium and Rhizoctonia on the plates selected and applied in pots experiments growing cucumber. Streptomyces isolates and their mutants gave high inhibition percentage for the three (the studied) pathogenic fungi compared with the control. In soil infested with Pythium deberianum and Rhizoctonia solani treatments with mutants 6, 7 and Topsin-M70 fungicide increased the percentage of survival plants by up to 85% compared with the control (40%). While the mutants 5. With Fusarium oxysporium 75 %. survival plants. While mutant 4 with Rhizoctonia solani and 2 and 9 Fusarium oxysporium had the lowest effect on increasing cucumber survival (55%) and mutant 2,8 on Pythium deberianum gave 60% compared with other treatments and control. This finding suggests that Streptomyces could be useful in protecting plants such as cucumbers from fungi and reducing environmental contamination with herbicides.

Research paper thumbnail of Molecular genetic studies on Atropa belladonna L. under effect of laser radiation and salicylic acid on Atropine, Scopolamine and Hyoscyamine by tissue culture techniques

African Journal of Biological Sciences

Research paper thumbnail of Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

African Journal of Biological Sciences

Many important substances are produced by some organisms, such as extracellular polysaccharides (... more Many important substances are produced by some organisms, such as extracellular polysaccharides (EPS). The genus Bacillus is an important one in this field. As a result, in this study, a defined strain of Bacillus sp. BAB3450 (Gen Bank) was used to produce EPS with the goal of increasing productivity by introducing acridine orange mutations (AO). The results were obtained from three mutations that were more productive than the wild type. On the other hand, using primers Inter-Simple Sequence Repeat (ISSR) and Start Codon Targeted Polymorphism (SCoT), a comparison with the three mutants and wild type were made at the molecular level. Eleven ISSR primers were used. The results showed that ISSR-19, with 92.86% polymorphism, was the best primer. Using ten SCoT primers, the results showed that SCoT-2 was the best primer, with 90% polymorphism. For the ISSR primer and SCoT primers, the wild type and three mutants were divided into two major groups based on marker analysis with distance 60 to 75% and 55 to 65%, respectively. Cluster analyses were used to create a dendrogram of Bacillus strains and mutants, revealing high genetic variations between the wild type and mutants. Finally, extracellular polysaccharides (EPS) have a wide range of industrial applications in biomedicine, such as anticoagulant. The EPS from wild type and three mutants were tested for anticoagulant comparing with heparin. Mutant No.1 gave the best anticoagulant (15˃%), while the wild type, mutant No.2, mutant No.3 and mutant No.4 gave 10˃% comparing with heparin (80˃%).

Research paper thumbnail of Genetic Diversity Based on SSR Markers and Morphological Analysis of faba bean (Vicia faba L.) in Egypt

Taeckholmia, 2020

Vicia faba L. is the most vital legumes and widely cultivated in all the world. Cultivation of to... more Vicia faba L. is the most vital legumes and widely cultivated in all the world. Cultivation of tolerant genetic variants is the correct way to avoid the influences of drought on crops. Allocated space adequacy of genetic difference in commercial bean cultivars. Molecular markers are benfial tools for choice among sorts. Therefore, the aim of this study was to evaluate the genetic and morphological variations to for further application in plant breeding research. In this study, five simple sequence repeats (SSR) primers were used to explain genetic and morphological diversity from 18 Egyptian faba beans cultivars were collected from different places in Egypt; 14 lines from different population in addition Giza 2, Giza 3 and Giza 716 and T.W from Sudan. The results of this study SSR markers are effective in estimating the germplasm of Egyptian beans. SSR markers results ranged from 15 bp to 202 bp with average of allele number 9.2 allele/markers. In addition, the morphological variati...

Research paper thumbnail of Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources

Polymers, 2021

Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) p... more Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the pr...

Research paper thumbnail of Genetic Diversity of Local Cowpea (Vigna spp. (L.) Walp.) Accessions Cultivated in Some Regions of Egypt

Jordan Journal of Biological Sciences, 2021

This work described the ten cowpea accessions using morphological, agronomical, and molecular cha... more This work described the ten cowpea accessions using morphological, agronomical, and molecular characterization and select the desirable cowpea accession for improvement. Twenty-four characteristics were quantitative attributes. Data demonstrated by the Principal Component Analysis (PCA) increasing in some components associated with a decrease in eigenvalues, which gave its max at three factors. The PCA was arranged into three main components, which altogether valued at 63.40%. The matrix of PCA data is standardized. The coordinates were calculated for Bi-plot mapping using the Perceptual Mapping (PERMAP). Radiata accessions (1 and 2) fell into a group. Unguiculata accessions are classified further into two sub-groups. The accessions (4 and 6) dropped into a sub-group, and the rest gathered into another sub-group. The PCR-based marker technique, SSR markers, were also analyzed. These markers gave a total of 64 alleles with 51.32% of polymorphism. A total of 26 alleles were polymorphi...

Research paper thumbnail of Genetic diversity and relationship between Egyptian Vigna (Vigna spp. (L.) Walp.) taxa populations via Phenotypic and Molecular profiling

Vegetos/Vegetos - International journal of plant research, Mar 18, 2024

Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.

Research paper thumbnail of Responses of He-Ne Laser Irradiation on Agronomical Characters and Cytological of (Atropa Belladonna L.)

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

The goal of the present work was to investigate the influence of laser irradiation on in vitro an... more The goal of the present work was to investigate the influence of laser irradiation on in vitro and in vivo growth agro-morphological criteria, mitotic activities and the chromosomes on Atropa belladonna L. The influence of laser radiation on Atropa seeds was investigated using helium-neon (He-Ne) were exposed to levels of laser doses (0.0,10,15, 20, 25 and 30 J cm)-2 using 7 m W He-Ne laser (632.8 nm),with a power density of 4.02 m W cm) 2 .The most of morphological, floral parameters germination percentage, plant height (cm), no. of leaves per plant, no. of branches per plant, leaf Length, width of leaf, leaf area and root length were studied in, First, Second and third months. The results showed significant differences among the five doses of laser radiation on seed germination, seedling height, plant height, no. of Leaves per plant at 10,20 and 30 days. The best doses 25 Jcm-2 to have a stimulating effect on these traits. The cytogenetic effects of physical mutagens Laser beam (0.0,10,15, 20, 25 and 30 J cm)-2 were investigated on the root tip cells of Atropa belladonna L., Various types of chromosomal aberrations (multinucleate cells) were observed during the cytological analysis of the root tip cells of both treatments. On the other hand, the high doses caused significant reduction as comparing with the control for all studied traits.

Research paper thumbnail of Molecular Genetic Studies on (Ficus Palmata & Ficus Carica Subsp Rupestris.) Under the Effect of Gamma Radiation by Tissue Culture Techniques

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

Ficus spp. are used as medicine and are used to reducing the risk of cancer and heart disease. Th... more Ficus spp. are used as medicine and are used to reducing the risk of cancer and heart disease. The phytochemical screening of the Ficus palmata plant extracts showed the presence of alkaloids, tannins, flavonoids, terpenoids and cardiac glycosides. This investigation was undertaken by using tissue culture technique on Ficus palmata & Ficus carica subsp rupestris. Study the effect of gamma rays doses of 0.0, 20, 30 and 40 Gy on callus production and the secondary plant constituents. The highest callus fresh weightand callus induction were achieved by callus derived from (leaves & stem) explants, which were cultured on (MS) medium supplemented with different concentrations of 2,4-D at 2.0 (mg/l) and Kin at 0.2 (mg/l). Polymerase chain reaction (PCR) amplification using eight ISSR primers showed polymorphic patterns with different response to gamma radiation doses. found genetic differences at the molecular level using ISSR with gamma doses compared to control. The total number of polymorphic bands was 239 bands (99.58% polymorphism). Found that there was genetic stability between the mother plants and product tissue culture for plants (Ficus palmata & Ficus carica subsp rupestris). The chemical composition of Ficus palmata was studied by means of GC-MS analysis under the effect of gamma radiation. The best doses of gamma were 30 & 40 Gy, as they increased the secondary compounds of Ficus palmata.

Research paper thumbnail of Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

African Journal of Biological Sciences, May 13, 2022

Research paper thumbnail of Evaluation of genetic stability using SCoT markers and SDS-PAGE with gamma radiation on callus of (Atropa belladonna L.) and antioxidant activity

Al-Azhar Journal of Agricultural Research (Online), Dec 1, 2021

Due to the improvement of the mutant germplasm and its use in the creation of new mutant organism... more Due to the improvement of the mutant germplasm and its use in the creation of new mutant organisms, these mutations have been instrumental in meeting global food and nutritional protection challenges. Mutagenic therapies for plant breeding and for drug alkaloids development projects have contributed to a wide range of genetic variability. The primary commercial source of drug alkaloids such as atropine is Atropa belladonna. This paper focuses on the effect of gamma rays (γ-rays) on callus of Atropa belladonna at the molecular level and changes of biochemical metabolisms in callus. Atropa belladonna callus were irradiated with 0.0, 30, 60, 90, 120 and 150 Gy gamma rays after 6 weeks on induction media. Highly significant differences between effect of gamma rays (γ-rays) on callus of Atropa belladonna at 2,4-D 2 mg/L and Kin 0.5 mg/L respectively were recorded on callus fresh weight and callus dry weight. 120 Gy had higher callus fresh weight, dry weight, Dry matter content (%) than the control. The difference in ion levels were measured in control and callus irradiated. The results clearly decrease in ion levels with increase of gamma dose. On the other hand, the deferent of antioxidant activity were cleared between control and gamma ray's callus. The hist antioxidant activity dose 150 Gy which recorded 30.20 comparing with control 20.10. Polyacrylamide gel electrophoresis SDS-protein PAGE profiles were used. In irradiated plants, there were several new protein bands that could be used as markers for each dose. Using DNA-Start codon targeted (SCoT) polymorphism assay. There were two negative molecular markers which were found only in control as compared to irradiated callus, with molecular sizes 940 and 400 bp.

Research paper thumbnail of Detection aflatoxin production by local isolates of Aspergillus spp. and molecular characterization

Archives of Agriculture Sciences Journal, Dec 1, 2021

Five isolates of Aspergillus spp. were isolated from stored wheat cereal and peanuts (groundnuts)... more Five isolates of Aspergillus spp. were isolated from stored wheat cereal and peanuts (groundnuts) in the El-Monofiya governorate in Egypt. The isolates were identified using morphological and microscopic properties. The ITS (internally transcribed spacer) region was found in the location of 600 bp. Results compared with data in GenBank confirmed that three isolates belonged to Aspergillus flavus and two isolates belonged to Aspergillus parasiticus. Three strains of Aspergillus flavus (Af1, Af2 and Af3) and two isolates of Aspergillus parasiticus (Ap4 and Ap5) were tested to produce aflatoxin on Czapek's agar medium. To distinguish between aflatoxigenic and non-aflatoxigenic isolates, TLC (thin layer chromatography) and PCR (polymerase chain reaction) were used. The TLC technique was previously used to quantify aflatoxin (B1, B2, G1 and G2) of five strains of Aspergillus. Three strains Af1, Af2 and Af3 gave B2 (50 μg/kg). The other strains Ap4 and Ap5 gave B1 (50 μg/kg) and G1 (75 μg/kg) for Ap4 and G2 (100 μg/kg) for Ap5. The DNA from all isolates has been extracted and amplified by PCR to encode target genes for the development of toxins (omt-A). It was observed in 3 (60%) of isolates Af1, Af2 & Af3 that the genes O-methyltransferase gene (omt-A) 300 bp. In addition, four SCoT primers and six ISSR primers were used to genetically link five Aspergillus spp. All five strains were divided into two groups for ISSR and SCoT primers. ISSR and SCOT analysis yielded similar results with TLC and aflatoxin-specific genes.

Research paper thumbnail of Studies on Genetic Selection to Produce New Genotypes of Mung bean (Vigna Radiata L.) by Gamma Rays and SCoT Marker

Al-Azhar Journal of Agricultural Research

A power of genetic selection is for producing new pure strains of interest species. The interest ... more A power of genetic selection is for producing new pure strains of interest species. The interest genes that are specifically expressed must be identified to develop the criterion of selection. The short period of Mung bean development could adapt to a broad sense of different factors such as agro-and ecosystems , plant rotation, plantation, and variety of germplasm. Three field experiments were carried out under the condition of Egyptian soil between Branch of

Research paper thumbnail of 16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

Archives of Agriculture Sciences Journal, 2019

Cucumber is one of Egypt's most popular vegetables, although it is sensitive to a number of plant... more Cucumber is one of Egypt's most popular vegetables, although it is sensitive to a number of plant diseases. The current study aimed to develop superior Streptomyces bacteria for improved antagonistic to fungi using mutagenic agents. Two Streptomyces isolates were isolated from Egyptian soil and identified by biochemical methods according to Bergey's Manual and partial 16S rRNA sequencing. These isolates were phenotypically identified as Streptomycetes genera. Mutation experiment by using chemical ethyl methanesulfonate (EMS) mutation methods. Through mutagenesis by (EMS) out of 10 obtained mutants. The mutants of Streptomyces isolates gave height antagonism of cucumber plant fungi Fusarium, Pythium and Rhizoctonia on the plates selected and applied in pots experiments growing cucumber. Streptomyces isolates and their mutants gave high inhibition percentage for the three (the studied) pathogenic fungi compared with the control. In soil infested with Pythium deberianum and Rhizoctonia solani treatments with mutants 6, 7 and Topsin-M70 fungicide increased the percentage of survival plants by up to 85% compared with the control (40%). While the mutants 5. With Fusarium oxysporium 75 %. survival plants. While mutant 4 with Rhizoctonia solani and 2 and 9 Fusarium oxysporium had the lowest effect on increasing cucumber survival (55%) and mutant 2,8 on Pythium deberianum gave 60% compared with other treatments and control. This finding suggests that Streptomyces could be useful in protecting plants such as cucumbers from fungi and reducing environmental contamination with herbicides.

Research paper thumbnail of Molecular genetic studies on Atropa belladonna L. under effect of laser radiation and salicylic acid on Atropine, Scopolamine and Hyoscyamine by tissue culture techniques

African Journal of Biological Sciences

Research paper thumbnail of Mutagenic effect of acridine orange on the production of EPS in Bacillus sp., its applications as anticoagulant and genetic diversity by ISSR and SCoT technique

African Journal of Biological Sciences

Many important substances are produced by some organisms, such as extracellular polysaccharides (... more Many important substances are produced by some organisms, such as extracellular polysaccharides (EPS). The genus Bacillus is an important one in this field. As a result, in this study, a defined strain of Bacillus sp. BAB3450 (Gen Bank) was used to produce EPS with the goal of increasing productivity by introducing acridine orange mutations (AO). The results were obtained from three mutations that were more productive than the wild type. On the other hand, using primers Inter-Simple Sequence Repeat (ISSR) and Start Codon Targeted Polymorphism (SCoT), a comparison with the three mutants and wild type were made at the molecular level. Eleven ISSR primers were used. The results showed that ISSR-19, with 92.86% polymorphism, was the best primer. Using ten SCoT primers, the results showed that SCoT-2 was the best primer, with 90% polymorphism. For the ISSR primer and SCoT primers, the wild type and three mutants were divided into two major groups based on marker analysis with distance 60 to 75% and 55 to 65%, respectively. Cluster analyses were used to create a dendrogram of Bacillus strains and mutants, revealing high genetic variations between the wild type and mutants. Finally, extracellular polysaccharides (EPS) have a wide range of industrial applications in biomedicine, such as anticoagulant. The EPS from wild type and three mutants were tested for anticoagulant comparing with heparin. Mutant No.1 gave the best anticoagulant (15˃%), while the wild type, mutant No.2, mutant No.3 and mutant No.4 gave 10˃% comparing with heparin (80˃%).

Research paper thumbnail of Genetic Diversity Based on SSR Markers and Morphological Analysis of faba bean (Vicia faba L.) in Egypt

Taeckholmia, 2020

Vicia faba L. is the most vital legumes and widely cultivated in all the world. Cultivation of to... more Vicia faba L. is the most vital legumes and widely cultivated in all the world. Cultivation of tolerant genetic variants is the correct way to avoid the influences of drought on crops. Allocated space adequacy of genetic difference in commercial bean cultivars. Molecular markers are benfial tools for choice among sorts. Therefore, the aim of this study was to evaluate the genetic and morphological variations to for further application in plant breeding research. In this study, five simple sequence repeats (SSR) primers were used to explain genetic and morphological diversity from 18 Egyptian faba beans cultivars were collected from different places in Egypt; 14 lines from different population in addition Giza 2, Giza 3 and Giza 716 and T.W from Sudan. The results of this study SSR markers are effective in estimating the germplasm of Egyptian beans. SSR markers results ranged from 15 bp to 202 bp with average of allele number 9.2 allele/markers. In addition, the morphological variati...

Research paper thumbnail of Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources

Polymers, 2021

Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) p... more Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the pr...

Research paper thumbnail of Genetic Diversity of Local Cowpea (Vigna spp. (L.) Walp.) Accessions Cultivated in Some Regions of Egypt

Jordan Journal of Biological Sciences, 2021

This work described the ten cowpea accessions using morphological, agronomical, and molecular cha... more This work described the ten cowpea accessions using morphological, agronomical, and molecular characterization and select the desirable cowpea accession for improvement. Twenty-four characteristics were quantitative attributes. Data demonstrated by the Principal Component Analysis (PCA) increasing in some components associated with a decrease in eigenvalues, which gave its max at three factors. The PCA was arranged into three main components, which altogether valued at 63.40%. The matrix of PCA data is standardized. The coordinates were calculated for Bi-plot mapping using the Perceptual Mapping (PERMAP). Radiata accessions (1 and 2) fell into a group. Unguiculata accessions are classified further into two sub-groups. The accessions (4 and 6) dropped into a sub-group, and the rest gathered into another sub-group. The PCR-based marker technique, SSR markers, were also analyzed. These markers gave a total of 64 alleles with 51.32% of polymorphism. A total of 26 alleles were polymorphi...