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Veterinary Pathology, 2020
Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea... more Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream ( Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hinder its research. This study investigated the associated lesions, its detection by quantitative polymerase chain reaction, and the cellular immune response of naturally infected fish. The intensity of infection in the intestine was correlated with stunted growth and reduced body condition. At the beginning of the outbreaks, infection prevalence was highest in intestine and stomach, and in subsequent months, the prevalence decreased in the intestine and increased in hematopoietic organs and stomach. In heavy infections, the intestine had histologic lesions of enterocyte hypercellularity and proliferation of rodlet cells. Infected enterocytes had E. nucleophila spores in the cytoplasm, and a pyknotic nucleus, karyorhexis or karyolysis. Lymphocytes were present at the base of the mucosa, and...
Journal of Fish Diseases, 2019
Microsporidians are minute intracellular parasites that infect all animal phyla and even protists... more Microsporidians are minute intracellular parasites that infect all animal phyla and even protists like amoebae and gregarines (Larsson, 2000; Scheid, Zöller, Pressmar, Richard, & Michel, 2008). Over half of the known genera infect aquatic organisms (Stentiford et al., 2016). With a close phylogenetic relationship with fungi, they share biological features such as the presence of chitin in the spores, the use of trehalose as the main sugar reserve or some similarities during mitosis and meiosis (Han & Weiss, 2017). Like in the Rozellomycota, adaption to obligate intracellular parasitism has led to gene loss, genome reduction and dependence upon the host for cell function (Ndikumana et al., 2017; Quandt et al., 2017). While many species tend to be considered secondary pathogens or linked to immunocompromised condition in human or veterinary medicine, numerous microsporidioses are involved in serious losses in aquaculture set
Veterinary Pathology, 2020
Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea... more Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream ( Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hinder its research. This study investigated the associated lesions, its detection by quantitative polymerase chain reaction, and the cellular immune response of naturally infected fish. The intensity of infection in the intestine was correlated with stunted growth and reduced body condition. At the beginning of the outbreaks, infection prevalence was highest in intestine and stomach, and in subsequent months, the prevalence decreased in the intestine and increased in hematopoietic organs and stomach. In heavy infections, the intestine had histologic lesions of enterocyte hypercellularity and proliferation of rodlet cells. Infected enterocytes had E. nucleophila spores in the cytoplasm, and a pyknotic nucleus, karyorhexis or karyolysis. Lymphocytes were present at the base of the mucosa, and...
Journal of Fish Diseases, 2019
Microsporidians are minute intracellular parasites that infect all animal phyla and even protists... more Microsporidians are minute intracellular parasites that infect all animal phyla and even protists like amoebae and gregarines (Larsson, 2000; Scheid, Zöller, Pressmar, Richard, & Michel, 2008). Over half of the known genera infect aquatic organisms (Stentiford et al., 2016). With a close phylogenetic relationship with fungi, they share biological features such as the presence of chitin in the spores, the use of trehalose as the main sugar reserve or some similarities during mitosis and meiosis (Han & Weiss, 2017). Like in the Rozellomycota, adaption to obligate intracellular parasitism has led to gene loss, genome reduction and dependence upon the host for cell function (Ndikumana et al., 2017; Quandt et al., 2017). While many species tend to be considered secondary pathogens or linked to immunocompromised condition in human or veterinary medicine, numerous microsporidioses are involved in serious losses in aquaculture set