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Biochimica Et Biophysica Acta-biomembranes, 1993
Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when A... more Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when ATP is regenerated by nutrient supplementation. In the presence of the sulfhydryl reducing agent dithiothreitol (DTT), this shape reversal is exaggerated, proceeding beyond normal discoid morphology to stomatocytic forms. DTT-induced stomatocytosis does not correlate consistently with alterations in cell ATP, spectrin phosphorylation, or phosphoinositide metabolism (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). The effect of Dq'T on outer-to-inner-monolayer transport of aminophospholipids was examined by monitoring shape changes induced by dilauroylphosphatidylserine (DLPS). Stomatocytosis induced by transport of this exogenous lipid to the membrane inner monolayer is accelerated and exaggerated by DTI'. The effect of DTI" on DLPS translocation is reversible and temperature dependent, consistent with the intervention of reducing agents in the activity of the aminophospholipid translocator. These findings bear on the relationship between cell redox status and shape regulation.
Biochimica Et Biophysica Acta-biomembranes, 1993
The echinocyte-to-discocyte shape recovery of metabolically depleted erythrocytes is compromised ... more The echinocyte-to-discocyte shape recovery of metabolically depleted erythrocytes is compromised by sulfhydryl reducing agents (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). In the presence of dithiothreitol (DTI') and sugars, crenated cells recover normal discoid shape transiently, but then develop the invaginations and intracellular inclusions of stomatocytes. The stomatogenic effects of D'I'T were investigated in erythrocytes recovering from crenation induced by several independent mechanisms. Cells crenated by direct manipulation of the membrane bilayer (lysophosphatidylcholine incorporation) recovered discoid shape similarly in the presence and absence of the reducing agent. In contrast, resealed ghosts and cells crenated by Mg 2÷ depletion or Ca 2÷ loading did not maintain stable discoid morphology in the presence of DTT, proceeding further to form stomatocytes. Thus cell crenation by expedients that involve cellular metabolic processes develop a redox-related morphological instability that is not found in amphipath-crenated cells.
Biochimica Et Biophysica Acta-biomembranes, 1993
Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when A... more Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when ATP is regenerated by nutrient supplementation. In the presence of the sulfhydryl reducing agent dithiothreitol (DTT), this shape reversal is exaggerated, proceeding beyond normal discoid morphology to stomatocytic forms. DTT-induced stomatocytosis does not correlate consistently with alterations in cell ATP, spectrin phosphorylation, or phosphoinositide metabolism (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). The effect of Dq'T on outer-to-inner-monolayer transport of aminophospholipids was examined by monitoring shape changes induced by dilauroylphosphatidylserine (DLPS). Stomatocytosis induced by transport of this exogenous lipid to the membrane inner monolayer is accelerated and exaggerated by DTI'. The effect of DTI" on DLPS translocation is reversible and temperature dependent, consistent with the intervention of reducing agents in the activity of the aminophospholipid translocator. These findings bear on the relationship between cell redox status and shape regulation.
Biochimica Et Biophysica Acta-biomembranes, 1993
The echinocyte-to-discocyte shape recovery of metabolically depleted erythrocytes is compromised ... more The echinocyte-to-discocyte shape recovery of metabolically depleted erythrocytes is compromised by sulfhydryl reducing agents (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). In the presence of dithiothreitol (DTI') and sugars, crenated cells recover normal discoid shape transiently, but then develop the invaginations and intracellular inclusions of stomatocytes. The stomatogenic effects of D'I'T were investigated in erythrocytes recovering from crenation induced by several independent mechanisms. Cells crenated by direct manipulation of the membrane bilayer (lysophosphatidylcholine incorporation) recovered discoid shape similarly in the presence and absence of the reducing agent. In contrast, resealed ghosts and cells crenated by Mg 2÷ depletion or Ca 2÷ loading did not maintain stable discoid morphology in the presence of DTT, proceeding further to form stomatocytes. Thus cell crenation by expedients that involve cellular metabolic processes develop a redox-related morphological instability that is not found in amphipath-crenated cells.