iliass lahmass - Academia.edu (original) (raw)
Papers by iliass lahmass
Evidence-Based Complementary and Alternative Medicine
The most costly spice in the world, Crocus sativus L. (C. sativus), has been used for more than 3... more The most costly spice in the world, Crocus sativus L. (C. sativus), has been used for more than 3,000 years. It has various beneficial applications in a range of fields, including aromas, colorants, and medications, but its usefulness as a food flavoring and coloring ingredient is the highest. Large quantities of by-products from the processing of saffron are typically thrown as unwanted bio-residues. This study’s goal was to assess and compare the nephroprotective effects of hydroethanolic extracts of C. sativus stigmas, tepals, and leaves on gentamicin (GM)-induced nephrotoxicity in rats. For that, we used a biochemical and histological investigation to propose new pharmaceutical valorizations. Based on the biochemical and histological analyses, it is concluded that all the studied parts of C sativus showed a renoprotective effect. Markedly, tepals revealed the most significant reduction of relative liver weight p < 0.05 , water intake p < 0.05 , plasma creatinine p < 0.0...
Antioxidants
The present study investigated the antioxidant activity, metal chelating ability and genoprotecti... more The present study investigated the antioxidant activity, metal chelating ability and genoprotective effect of the hydroethanolic extracts of Crocus sativus stigmas (STG), tepals (TPL) and leaves (LV). We evaluated the antioxidant and metal (Fe2+ and Cu2+) chelating activities of the stigmas, tepals and leaves of C. sativus. Similarly, we examined the genotoxic and DNA protective effect of these parts on rat leukocytes by comet assay. The results showed that TPL contains the best polyphenol content (64.66 µg GA eq/mg extract). The highest radical scavenging activity is shown by the TPL (DPPH radical scavenging activity: IC50 = 80.73 µg/mL). The same extracts gave a better ferric reducing power at a dose of 50 µg/mL, and better protective activity against β-carotene degradation (39.31% of oxidized β-carotene at a 100 µg/mL dose). In addition, they showed a good chelating ability of Fe2+ (48.7% at a 500 µg/mL dose) and Cu2+ (85.02% at a dose of 500 µg/mL). Thus, the antioxidant activit...
Escherichia coli is involved in many diseases. Its high sensitivity to its envir antibiotic resis... more Escherichia coli is involved in many diseases. Its high sensitivity to its envir antibiotic resistance mechanisms against the most p werful antibiotics. resistance status of avian Escherichia coli intestine (cecum) were taken in the various Oujda t raditional slaughterhouses over a period of twenty each sample, a single Escherichia coli strain was isolated and purified on MacConckey of these strains to twentyone different antibiotics was tested on Mueller Hin ton agar using commercial antibiotic disks. resistance levels were recorde d with alarming rates for antibiotics used preventi vely in poultry, Flumequine (94%), Thiamphenicol (78%), Tetracycline (58%), Amoxicillin (25 %), Sulf amethoxazole/Trimethoprim (55%), Streptomycin (51%) , Amoxicillin/Clavulani Acid (47%), Enrofloxacin (37%), Ciprofloxcin (35%), Doxycycline (51%), Florfenic ol (32%) and Gentamycin (3%).These high prevalences explain, to a large extent, treatment f ailures observed in human and veterinary medicine a nd c...
Mouad El Khoudri*, Iliass Lahmass, Mohammed Ammari, Dounia El Adak, Mohamed Ahlalouch, Abdelkarim... more Mouad El Khoudri*, Iliass Lahmass, Mohammed Ammari, Dounia El Adak, Mohamed Ahlalouch, Abdelkarim Chamrar, Laïla Ben Allal [1] Research Team: Materials, Environment and Sustainable Development (MEDD), Faculty of Sciences and Techniques of Tangier, BP 416 – Tangier, Morocco [2] Laboratory of biochemistry and biotechnology, University 1st, Oujda, Morocco [3] Laboratory of applied geosciences, University Mohammed 1, Oujda, Morocco
Medicinal and Aromatic plants, 2021
The medicinal uses of saffron ( Crocus sativus Linnaeus) have a long history beginning in Asian c... more The medicinal uses of saffron ( Crocus sativus Linnaeus) have a long history beginning in Asian countries since the Late Bronze Age. Recent studies have validated its potential to lower the risk of several diseases. Saffron has been suggested to be effective in the treatment of a wide range of disorders including coronary artery diseases, hypertension, stomach disorders, dysmenorrhea and learning and memory impairments. In addition, different studies have indicated that saffron has anti-inflammatory, anti-atherosclerotic, antidiabetic, antigenotoxic and cytotoxic activities. The anticonvulsant and anti-Alzheimer properties of saffron extract were shown in human and animal studies. The efficacy of Crocus sativus in the treatment of mild to moderate depression was also reported in clinical trial. Many of these medicinal properties of saffron can be attributed to a number of its compounds such as crocetin, crocins and other substances having strong antioxidant and radical scavenger pro...
The ecotoxicology is the science which studies the effects of the chemical, physical and biologic... more The ecotoxicology is the science which studies the effects of the chemical, physical and biological factors on all the human beings. Genotoxicity is a word in genetics defined as a destructive effect on a cell's genetic material affecting its integrity. The assessment of genotoxicity represents an essential component of the safety assessment of all types of substances. Regulatory authorities all over the world require data on the genotoxic potential of new drugs, as part of the safety evaluation process. The pre-clinical studies are generally conducted to obtain the basic toxicological profile of new chemical entities. So, the place of the genotoxicity in the field of the ecotoxicology turns out very interesting and very promising. The detection of DNA damage at the level of individual eukaryotic cell warrants significance in fields of toxicology, pharmaceutical genotoxicity testing, environmental/human biomonitoring, diagnosis of genetic disorders etc. This mini review correspo...
Physiology and Pharmacology, 2017
Introduction: In this study, we evaluated the antidiabetic and antidiabetogenic effect of saffron... more Introduction: In this study, we evaluated the antidiabetic and antidiabetogenic effect of saffron against diabetes induced by artificial dye tartrazine on normal male rats. Methods: Dried saffron was macerated for 12 hours in distilled water before usage and crude extract was used to treat male rats. Rats were divided into 5 groups consisted of 6 rats and treatment was performed daily and orally. Group-1 treated with distilled water, group-2 with tartrazine 10mg/kg followed by saffron 120mg/kg until the last day of treatment, group-3 administered with tartrazine, group-4 treated with saffron for 60 days and administered with tartrazine, group-5 with saffron for 105 days. Levels of blood glucose and body weight have been evaluated every 10 days and clinical demonstrations and metabolic parameters were evaluated at the end of the experiment. Results: Levels of blood glucose and body weight have been evaluated every 10 days and clinical demonstrations and metabolic parameters were eval...
The present study deals determination of Tartrazine, which is known as an azo dye, present in thr... more The present study deals determination of Tartrazine, which is known as an azo dye, present in three different kinds of yellow foodstuffs: solid juice powders, solid jelly powders and soft drinks commercially available food items inOujda-Moroccomarkets. Tartrazine present in different kinds of yellow foodstuffs was determined by high-performance liquid chromatography with UV-DAD detection. The following food dye was analyzed within less than 10 min using analytical C18 column (4.6 ×150 mm, 10 μm) at ambient temperature. This method has potential to be used for Tartrazine due to its rapidness, simplicity, reliability, and sensitivity. All studied samples showed dye levels in according to the legislations adopted by the official food control laboratories inMorocco.
Introduction: The present study investigated for the first time, the antihyperglycemic effect of ... more Introduction: The present study investigated for the first time, the antihyperglycemic effect of tepals and leaves of Crocus sativus in streptozotocin-induced diabetic rats. The effect of these by-products were compared with that of saffron stigma and glibenclamide. Methods: Hydroethanolic extracts (stigmas, tepals and leaves) and glibenclamide were administered orally in aqueous solution daily for 21 days. In the present study, 36 male rats were used. Six rats were used in each group. Group 1: normal control rats (N), received distilled water; group 2: diabetic control rats (D), received distilled water; group 3: treated diabetic rats (TPL), received tepal (TPL) extract; group 4: treated diabetic rats (STG), received stigma (STG) extract; group 5: treated diabetic rats (LF), received leaf (LF) extract and group 6: treated diabetic rats (GLB), received glibenclamide (GLB). Blood glucose, body weight, water intake, urine elimination, plasma triglycerides, cholesterol, urea, creatinin...
Physiology and Pharmacology, 2021
Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and ... more Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Ma...
Annals of Agricultural Sciences
Journal of Functional Foods
Journal of Medical and Allied Sciences, 2017
Waste and Biomass Valorization, 2017
Journal of Medical and Allied Sciences, 2017
Evidence-Based Complementary and Alternative Medicine
The most costly spice in the world, Crocus sativus L. (C. sativus), has been used for more than 3... more The most costly spice in the world, Crocus sativus L. (C. sativus), has been used for more than 3,000 years. It has various beneficial applications in a range of fields, including aromas, colorants, and medications, but its usefulness as a food flavoring and coloring ingredient is the highest. Large quantities of by-products from the processing of saffron are typically thrown as unwanted bio-residues. This study’s goal was to assess and compare the nephroprotective effects of hydroethanolic extracts of C. sativus stigmas, tepals, and leaves on gentamicin (GM)-induced nephrotoxicity in rats. For that, we used a biochemical and histological investigation to propose new pharmaceutical valorizations. Based on the biochemical and histological analyses, it is concluded that all the studied parts of C sativus showed a renoprotective effect. Markedly, tepals revealed the most significant reduction of relative liver weight p < 0.05 , water intake p < 0.05 , plasma creatinine p < 0.0...
Antioxidants
The present study investigated the antioxidant activity, metal chelating ability and genoprotecti... more The present study investigated the antioxidant activity, metal chelating ability and genoprotective effect of the hydroethanolic extracts of Crocus sativus stigmas (STG), tepals (TPL) and leaves (LV). We evaluated the antioxidant and metal (Fe2+ and Cu2+) chelating activities of the stigmas, tepals and leaves of C. sativus. Similarly, we examined the genotoxic and DNA protective effect of these parts on rat leukocytes by comet assay. The results showed that TPL contains the best polyphenol content (64.66 µg GA eq/mg extract). The highest radical scavenging activity is shown by the TPL (DPPH radical scavenging activity: IC50 = 80.73 µg/mL). The same extracts gave a better ferric reducing power at a dose of 50 µg/mL, and better protective activity against β-carotene degradation (39.31% of oxidized β-carotene at a 100 µg/mL dose). In addition, they showed a good chelating ability of Fe2+ (48.7% at a 500 µg/mL dose) and Cu2+ (85.02% at a dose of 500 µg/mL). Thus, the antioxidant activit...
Escherichia coli is involved in many diseases. Its high sensitivity to its envir antibiotic resis... more Escherichia coli is involved in many diseases. Its high sensitivity to its envir antibiotic resistance mechanisms against the most p werful antibiotics. resistance status of avian Escherichia coli intestine (cecum) were taken in the various Oujda t raditional slaughterhouses over a period of twenty each sample, a single Escherichia coli strain was isolated and purified on MacConckey of these strains to twentyone different antibiotics was tested on Mueller Hin ton agar using commercial antibiotic disks. resistance levels were recorde d with alarming rates for antibiotics used preventi vely in poultry, Flumequine (94%), Thiamphenicol (78%), Tetracycline (58%), Amoxicillin (25 %), Sulf amethoxazole/Trimethoprim (55%), Streptomycin (51%) , Amoxicillin/Clavulani Acid (47%), Enrofloxacin (37%), Ciprofloxcin (35%), Doxycycline (51%), Florfenic ol (32%) and Gentamycin (3%).These high prevalences explain, to a large extent, treatment f ailures observed in human and veterinary medicine a nd c...
Mouad El Khoudri*, Iliass Lahmass, Mohammed Ammari, Dounia El Adak, Mohamed Ahlalouch, Abdelkarim... more Mouad El Khoudri*, Iliass Lahmass, Mohammed Ammari, Dounia El Adak, Mohamed Ahlalouch, Abdelkarim Chamrar, Laïla Ben Allal [1] Research Team: Materials, Environment and Sustainable Development (MEDD), Faculty of Sciences and Techniques of Tangier, BP 416 – Tangier, Morocco [2] Laboratory of biochemistry and biotechnology, University 1st, Oujda, Morocco [3] Laboratory of applied geosciences, University Mohammed 1, Oujda, Morocco
Medicinal and Aromatic plants, 2021
The medicinal uses of saffron ( Crocus sativus Linnaeus) have a long history beginning in Asian c... more The medicinal uses of saffron ( Crocus sativus Linnaeus) have a long history beginning in Asian countries since the Late Bronze Age. Recent studies have validated its potential to lower the risk of several diseases. Saffron has been suggested to be effective in the treatment of a wide range of disorders including coronary artery diseases, hypertension, stomach disorders, dysmenorrhea and learning and memory impairments. In addition, different studies have indicated that saffron has anti-inflammatory, anti-atherosclerotic, antidiabetic, antigenotoxic and cytotoxic activities. The anticonvulsant and anti-Alzheimer properties of saffron extract were shown in human and animal studies. The efficacy of Crocus sativus in the treatment of mild to moderate depression was also reported in clinical trial. Many of these medicinal properties of saffron can be attributed to a number of its compounds such as crocetin, crocins and other substances having strong antioxidant and radical scavenger pro...
The ecotoxicology is the science which studies the effects of the chemical, physical and biologic... more The ecotoxicology is the science which studies the effects of the chemical, physical and biological factors on all the human beings. Genotoxicity is a word in genetics defined as a destructive effect on a cell's genetic material affecting its integrity. The assessment of genotoxicity represents an essential component of the safety assessment of all types of substances. Regulatory authorities all over the world require data on the genotoxic potential of new drugs, as part of the safety evaluation process. The pre-clinical studies are generally conducted to obtain the basic toxicological profile of new chemical entities. So, the place of the genotoxicity in the field of the ecotoxicology turns out very interesting and very promising. The detection of DNA damage at the level of individual eukaryotic cell warrants significance in fields of toxicology, pharmaceutical genotoxicity testing, environmental/human biomonitoring, diagnosis of genetic disorders etc. This mini review correspo...
Physiology and Pharmacology, 2017
Introduction: In this study, we evaluated the antidiabetic and antidiabetogenic effect of saffron... more Introduction: In this study, we evaluated the antidiabetic and antidiabetogenic effect of saffron against diabetes induced by artificial dye tartrazine on normal male rats. Methods: Dried saffron was macerated for 12 hours in distilled water before usage and crude extract was used to treat male rats. Rats were divided into 5 groups consisted of 6 rats and treatment was performed daily and orally. Group-1 treated with distilled water, group-2 with tartrazine 10mg/kg followed by saffron 120mg/kg until the last day of treatment, group-3 administered with tartrazine, group-4 treated with saffron for 60 days and administered with tartrazine, group-5 with saffron for 105 days. Levels of blood glucose and body weight have been evaluated every 10 days and clinical demonstrations and metabolic parameters were evaluated at the end of the experiment. Results: Levels of blood glucose and body weight have been evaluated every 10 days and clinical demonstrations and metabolic parameters were eval...
The present study deals determination of Tartrazine, which is known as an azo dye, present in thr... more The present study deals determination of Tartrazine, which is known as an azo dye, present in three different kinds of yellow foodstuffs: solid juice powders, solid jelly powders and soft drinks commercially available food items inOujda-Moroccomarkets. Tartrazine present in different kinds of yellow foodstuffs was determined by high-performance liquid chromatography with UV-DAD detection. The following food dye was analyzed within less than 10 min using analytical C18 column (4.6 ×150 mm, 10 μm) at ambient temperature. This method has potential to be used for Tartrazine due to its rapidness, simplicity, reliability, and sensitivity. All studied samples showed dye levels in according to the legislations adopted by the official food control laboratories inMorocco.
Introduction: The present study investigated for the first time, the antihyperglycemic effect of ... more Introduction: The present study investigated for the first time, the antihyperglycemic effect of tepals and leaves of Crocus sativus in streptozotocin-induced diabetic rats. The effect of these by-products were compared with that of saffron stigma and glibenclamide. Methods: Hydroethanolic extracts (stigmas, tepals and leaves) and glibenclamide were administered orally in aqueous solution daily for 21 days. In the present study, 36 male rats were used. Six rats were used in each group. Group 1: normal control rats (N), received distilled water; group 2: diabetic control rats (D), received distilled water; group 3: treated diabetic rats (TPL), received tepal (TPL) extract; group 4: treated diabetic rats (STG), received stigma (STG) extract; group 5: treated diabetic rats (LF), received leaf (LF) extract and group 6: treated diabetic rats (GLB), received glibenclamide (GLB). Blood glucose, body weight, water intake, urine elimination, plasma triglycerides, cholesterol, urea, creatinin...
Physiology and Pharmacology, 2021
Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and ... more Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Ma...
Annals of Agricultural Sciences
Journal of Functional Foods
Journal of Medical and Allied Sciences, 2017
Waste and Biomass Valorization, 2017
Journal of Medical and Allied Sciences, 2017