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Journal of Plant Physiology, 1996
Plants of napiergrass or elephantgrass (Pennisetum purpureum Schum.) were regenerated from embryo... more Plants of napiergrass or elephantgrass (Pennisetum purpureum Schum.) were regenerated from embryogenic calli derived from leaf and inflorescence segments, cell suspensions, protoplasts, and cryopreserved cell suspension cultures. The induction of somatic embryogenesis in segments of young leaves was found to be under developmental control, allowing a zone of cells competent for somatic embryogenesis to be mapped along the length of young leaves. Somatic embryogenesis was also initiated in segments of immature inflorescences. Factors important for the development of embryogenic suspension cultures were investigated. The starting callus tissues and gradual change of liquid medium were found to be critical for establishing embryogenic suspension cultures, which provided a critical source of protoplasts for the regeneration of plants. Plants regenerated from suspensions as well as protoplasts isolated from them grew to maturity in the field with normal morphology and chromosome numbers. Plants were also regenerated from cryopreserved cells after two and a half years of cryogenic storage, and established in soil. a quality forage for ruminants . Previously, plants of napiergrass have been regenerated from cultured leaf and inflorescence explants. Plantlets were also recovered at very low efficiency from protoplasts isolated from suspension cultures, but they neither continued growth nor could be transplanted to soil . In this report, we confirm the utility of leaf and inflorescence segments for the establishment of highly embryogenic callus cultures, which were utilized for the initiation of embryogenic cell suspension cultures. Plants regenerated from the suspensions as well as protoplasts isolated from them were grown to maturity in soil. Plants were also regenerated from cryopreserved cell suspensions after two and a half years of cryogenic storage.
Journal of Plant Physiology, 1996
Plants of napiergrass or elephantgrass (Pennisetum purpureum Schum.) were regenerated from embryo... more Plants of napiergrass or elephantgrass (Pennisetum purpureum Schum.) were regenerated from embryogenic calli derived from leaf and inflorescence segments, cell suspensions, protoplasts, and cryopreserved cell suspension cultures. The induction of somatic embryogenesis in segments of young leaves was found to be under developmental control, allowing a zone of cells competent for somatic embryogenesis to be mapped along the length of young leaves. Somatic embryogenesis was also initiated in segments of immature inflorescences. Factors important for the development of embryogenic suspension cultures were investigated. The starting callus tissues and gradual change of liquid medium were found to be critical for establishing embryogenic suspension cultures, which provided a critical source of protoplasts for the regeneration of plants. Plants regenerated from suspensions as well as protoplasts isolated from them grew to maturity in the field with normal morphology and chromosome numbers. Plants were also regenerated from cryopreserved cells after two and a half years of cryogenic storage, and established in soil. a quality forage for ruminants . Previously, plants of napiergrass have been regenerated from cultured leaf and inflorescence explants. Plantlets were also recovered at very low efficiency from protoplasts isolated from suspension cultures, but they neither continued growth nor could be transplanted to soil . In this report, we confirm the utility of leaf and inflorescence segments for the establishment of highly embryogenic callus cultures, which were utilized for the initiation of embryogenic cell suspension cultures. Plants regenerated from the suspensions as well as protoplasts isolated from them were grown to maturity in soil. Plants were also regenerated from cryopreserved cell suspensions after two and a half years of cryogenic storage.