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Papers by indu rani

Research paper thumbnail of Synthesis, Spectroscopic Characterization, Antioxidant Properties and Antimicrobial Activity of Transition Metal Complexes from Novel Schiff Base

Current Journal of Applied Science and Technology

Benzoylpyridine and nitro benzoic acid hydrazides were used to examine novel Schiff base Co(II), ... more Benzoylpyridine and nitro benzoic acid hydrazides were used to examine novel Schiff base Co(II), Mn(II), Fe(II), and Cu(II) complexes. Elemental analysis, molar conductance, magnetic measurements and other methods that like FT-IR, UV–Vis and 1H NMR studies have been used to deduce the nature of bonding and structure of these complexes. These studies revealed that the Co(II), Mn(II), Fe(II) and Cu(II) coordinated by four donor atoms in all the complexes. Free radical scavenging activity was determined according to the elimination of radicals from 2,2-diphenyl-1-picrylhydrazyl (DPPH). These compounds were compared to known antibiotics ciprofloxacin and fluconazole in their ability to inhibit the growth of pathogenic bacteria Bacillus subtilis (as gramme positive bacteria), Pseudomonas sp. (as gramme negative bacteria), and fungus Rhizoctonia sp., Alternaria sp., and Fusarium oxysporum. As an antibacterial and antioxidant agent, the complexes are more effective than the ligand. The cop...

Research paper thumbnail of Evaluation and comparison of the leaves and stem of Argemone mexicana L. in various solvents for total phenolics, total flavonoids and antioxidant activity

Annals of phytomedicine, Dec 1, 2022

The use of plant leaves and stem is a common practice in traditional medicine. The naturally avai... more The use of plant leaves and stem is a common practice in traditional medicine. The naturally available herbs are used for medicinal purpose to promote health. The leaves and stem of Argemone mexicana L. is one such herb used against different ailments. Studies on the antioxidant, phenolic and flavonoid content of the leaves and stem using various solvent extracts were; however, scarce for the Haryana region. The aim of the research was to assess the antioxidant activity, total phenolics and total flavonoids of A. mexicana leaves and stem. Using the Soxhlet apparatus, extracts were prepared using a variety of solvents, including ethyl acetate, acetone, water and methanol, based on variable polarity. Among all solvents, methanol extract had the highest concentration of total phenolics as well as total flavonoids in leaves compared to stem. A. mexicana leaves and stem displayed a wide range of DPPH free radical scavenging activity, which was higher at higher concentrations (100 mg/ml). The leaves component showed the greatest DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity with IC 50 values of 23.9 µg/ml in methanol extract and stem part with IC 50 values of 26.2 µg/ml in the same extract. FTIR analysis showed that the presence of phenols and flavanoids. In terms of antioxidant potency, higher amounts of total phenolics as well as total flavonoids in methanol extract of leaves and stem was shown to be the most potent antioxidant. The leaves and stem of A. mexicana have a considerable amount of flavanoids, phenolics and antioxidant activity.

Research paper thumbnail of Mineral content, bioactive ingredient identification and antioxidant activity of Argemone mexicana L. flowers extracts

Annals of Phytomedicine: An International Journal

Research paper thumbnail of Determination of total phenolic, free radical scavenging activity and antimicrobial activity of root extracts of Argemone mexicana L. in methanol solvent

Annals of Phytomedicine: An International Journal

Argemone mexicana L. (Papaveraceae), tropical yearly wild plant, called as Mexican prickly poppy ... more Argemone mexicana L. (Papaveraceae), tropical yearly wild plant, called as Mexican prickly poppy is a species of poppy, found in Mexico and now widely naturalized in many parts of the world with broad range of activities. The main aim of this study was to evaluate total phenolic content, antioxidant potential and antimicrobial activity in root part of A. mexicana. Root parts of A. mexicana were extracted with methanol using Soxhlet apparatus and further concentrated on rotatory vacuum evapoprator. The total phenolic content of methanol root extracts was quantified using the Folin-Ciocalteu method, antioxidant activity was assessed using the DPPH method, and antibacterial activity was tested using the agar well diffusion method. Total phenolics in root extracts were found to be 8.91 mg GAE/g. Methanolic root extract of A. mexicana showed that DPPH free radical scavenging activity varied widely and it increased with increase in concentration levels. The IC 50 value of the methanolic extract was lowest 24.98 µg/ml and indicating the highest DPPH free radical scavenging activity. The higher antifungal activity is exhibited by methanol root extracts as comparable to standard. Methanol root extract of A. mexicana was said to be valuable for antioxidant potential as well as for fine antifungal activity.

Research paper thumbnail of Effect of Extraction Techniques and Evaluation of Antimicrobial Activity of Argemone mexicana L. Leaves and Roots Extracts in Different Solvents

Journal of Scientific Research and Reports

Aim: The main objective of the study is to estimate the effect of extraction techniques and antim... more Aim: The main objective of the study is to estimate the effect of extraction techniques and antimicrobial activity of different solvent extract of A. mexicana leaves and roots. Study Design: The research is experimental in nature. Place and Duration of Study: Department of Chemistry and Microbiology at CCSHAU, Hisar, between late 2020 and January 2022. Methodology: The leaves and roots of A. mexicana were harvested for this study. Shade dried roots and leaves were cut into small pieces of 2-3 inches and processed into powder using a mixer grinder. Soxhlet extraction and microwave-assisted extraction procedures were used to extract leaves and roots in solvents such as acetone, methanol, and water. The antimicrobial activity of the roots and leaves extracts were evaluated against Gram +ve bacteria (Xanthomonas campesteris, Bacillus cereus, Staphylococcus aureus) and fungal species (Fusarium oxysporum, Macrophomina phaseolina and Candida albicans) and their zones of inhibition in mm a...

Research paper thumbnail of Synthesis, Spectroscopic Characterization, Antioxidant Properties and Antimicrobial Activity of Transition Metal Complexes from Novel Schiff Base

Current Journal of Applied Science and Technology

Benzoylpyridine and nitro benzoic acid hydrazides were used to examine novel Schiff base Co(II), ... more Benzoylpyridine and nitro benzoic acid hydrazides were used to examine novel Schiff base Co(II), Mn(II), Fe(II), and Cu(II) complexes. Elemental analysis, molar conductance, magnetic measurements and other methods that like FT-IR, UV–Vis and 1H NMR studies have been used to deduce the nature of bonding and structure of these complexes. These studies revealed that the Co(II), Mn(II), Fe(II) and Cu(II) coordinated by four donor atoms in all the complexes. Free radical scavenging activity was determined according to the elimination of radicals from 2,2-diphenyl-1-picrylhydrazyl (DPPH). These compounds were compared to known antibiotics ciprofloxacin and fluconazole in their ability to inhibit the growth of pathogenic bacteria Bacillus subtilis (as gramme positive bacteria), Pseudomonas sp. (as gramme negative bacteria), and fungus Rhizoctonia sp., Alternaria sp., and Fusarium oxysporum. As an antibacterial and antioxidant agent, the complexes are more effective than the ligand. The cop...

Research paper thumbnail of Evaluation and comparison of the leaves and stem of Argemone mexicana L. in various solvents for total phenolics, total flavonoids and antioxidant activity

Annals of phytomedicine, Dec 1, 2022

The use of plant leaves and stem is a common practice in traditional medicine. The naturally avai... more The use of plant leaves and stem is a common practice in traditional medicine. The naturally available herbs are used for medicinal purpose to promote health. The leaves and stem of Argemone mexicana L. is one such herb used against different ailments. Studies on the antioxidant, phenolic and flavonoid content of the leaves and stem using various solvent extracts were; however, scarce for the Haryana region. The aim of the research was to assess the antioxidant activity, total phenolics and total flavonoids of A. mexicana leaves and stem. Using the Soxhlet apparatus, extracts were prepared using a variety of solvents, including ethyl acetate, acetone, water and methanol, based on variable polarity. Among all solvents, methanol extract had the highest concentration of total phenolics as well as total flavonoids in leaves compared to stem. A. mexicana leaves and stem displayed a wide range of DPPH free radical scavenging activity, which was higher at higher concentrations (100 mg/ml). The leaves component showed the greatest DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity with IC 50 values of 23.9 µg/ml in methanol extract and stem part with IC 50 values of 26.2 µg/ml in the same extract. FTIR analysis showed that the presence of phenols and flavanoids. In terms of antioxidant potency, higher amounts of total phenolics as well as total flavonoids in methanol extract of leaves and stem was shown to be the most potent antioxidant. The leaves and stem of A. mexicana have a considerable amount of flavanoids, phenolics and antioxidant activity.

Research paper thumbnail of Mineral content, bioactive ingredient identification and antioxidant activity of Argemone mexicana L. flowers extracts

Annals of Phytomedicine: An International Journal

Research paper thumbnail of Determination of total phenolic, free radical scavenging activity and antimicrobial activity of root extracts of Argemone mexicana L. in methanol solvent

Annals of Phytomedicine: An International Journal

Argemone mexicana L. (Papaveraceae), tropical yearly wild plant, called as Mexican prickly poppy ... more Argemone mexicana L. (Papaveraceae), tropical yearly wild plant, called as Mexican prickly poppy is a species of poppy, found in Mexico and now widely naturalized in many parts of the world with broad range of activities. The main aim of this study was to evaluate total phenolic content, antioxidant potential and antimicrobial activity in root part of A. mexicana. Root parts of A. mexicana were extracted with methanol using Soxhlet apparatus and further concentrated on rotatory vacuum evapoprator. The total phenolic content of methanol root extracts was quantified using the Folin-Ciocalteu method, antioxidant activity was assessed using the DPPH method, and antibacterial activity was tested using the agar well diffusion method. Total phenolics in root extracts were found to be 8.91 mg GAE/g. Methanolic root extract of A. mexicana showed that DPPH free radical scavenging activity varied widely and it increased with increase in concentration levels. The IC 50 value of the methanolic extract was lowest 24.98 µg/ml and indicating the highest DPPH free radical scavenging activity. The higher antifungal activity is exhibited by methanol root extracts as comparable to standard. Methanol root extract of A. mexicana was said to be valuable for antioxidant potential as well as for fine antifungal activity.

Research paper thumbnail of Effect of Extraction Techniques and Evaluation of Antimicrobial Activity of Argemone mexicana L. Leaves and Roots Extracts in Different Solvents

Journal of Scientific Research and Reports

Aim: The main objective of the study is to estimate the effect of extraction techniques and antim... more Aim: The main objective of the study is to estimate the effect of extraction techniques and antimicrobial activity of different solvent extract of A. mexicana leaves and roots. Study Design: The research is experimental in nature. Place and Duration of Study: Department of Chemistry and Microbiology at CCSHAU, Hisar, between late 2020 and January 2022. Methodology: The leaves and roots of A. mexicana were harvested for this study. Shade dried roots and leaves were cut into small pieces of 2-3 inches and processed into powder using a mixer grinder. Soxhlet extraction and microwave-assisted extraction procedures were used to extract leaves and roots in solvents such as acetone, methanol, and water. The antimicrobial activity of the roots and leaves extracts were evaluated against Gram +ve bacteria (Xanthomonas campesteris, Bacillus cereus, Staphylococcus aureus) and fungal species (Fusarium oxysporum, Macrophomina phaseolina and Candida albicans) and their zones of inhibition in mm a...