ionan marigomez - Academia.edu (original) (raw)

Papers by ionan marigomez

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the effect of different salinity regime on the burial activity of Mya arenaria, as well as the combined effect of osmotic stress and repeated burrowing on the succinate dehydrogenase and ATPase activity in foot muscle of Mya arenaria.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the concentration on different amino acids and short chain organic acids in the whole body tissue of Mya arenaria.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the trypsin- and chymotrypsin-like proteasomal and lysosomal activity in gill tissue and digestive gland of Mya arenaria.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the activity leucine aminopeptidase in gill tissue and digestive gland of Mya arenaria.

Histology and histopathology, 2007

Metallothioneins (MT) were localized by immunochemistry in different organs and cell compartments... more Metallothioneins (MT) were localized by immunochemistry in different organs and cell compartments of turbot exposed to sublethal concentrations (100 ppb) of Cd for 7 days. The polyclonal rabbit anti-cod MT antibody (NIVA, Norway) applied herein exhibited positive cross-reactivity with turbot MTs. Immunoreactive MTs were localized in the branchial epithelium, in the liver and in the kidney of turbot. In Cd exposed fishes MTs were demonstrated mainly in branchial chloride cells (CC) and to a lesser extend in the area where progenitor cells are located and in the cells of the respiratory epithelium (secondary lamellae). A higher staining intensity for MTs was observed in CC of the interlamellar space of the main branchial epithelium in comparison with control CC. MT-staining was also observed in the chondroblasts of the cartilage and in the erythrocytes within blood vessels both in control and Cd-exposed specimens. MT immunoreaction was high in the liver hepatocytes and weak in the epi...

Bioturbators such as sediment-dwelling marine bivalves are ecosystem engineers that enhance sedim... more Bioturbators such as sediment-dwelling marine bivalves are ecosystem engineers that enhance sediment-water exchange and benthic-pelagic coupling. In shallow coastal areas, bivalves are exposed to frequent disturbance and salinity stress that might negatively affect their activity and physiological performance; however, the mechanisms underlying these effects are not fully understood. We investigated the effects of osmotic stress (low and fluctuating salinity) and repeated burrowing on aerobic and contractile capacity of the foot muscle (assessed by the activity of succinate dehydrogenase and myosin ATPase) as well as the levels of organic osmolytes (free amino acids) and biochemical markers of protein synthesis and proteolysis in key osmoregulatory and energy storing tissues (gills and hepatopancreas, respectively) in a common bioturbator, the soft shell clam Mya arenaria. Our findings indicate that exhaustive exercise and osmotic stress interactively affect whole-body protein homeostasis and functional capacity of the foot muscle in soft shell clams which might contribute to reduced burrowing activity of bivalve bioturbators in osmotically challenging environments such as estuaries and shallow coastal zones.

Frontiers in Microbiology, 2020

Mollusks-IMC International Collaboration IMC infection distributed among different taxa, includin... more Mollusks-IMC International Collaboration IMC infection distributed among different taxa, including the phylum Spirochetes, the families Anaplasmataceae and Simkaniaceae, the genera Mycoplasma and Francisella, and sulfur-oxidizing endosymbionts. Sequences like Francisella halioticida/philomiragia and Candidatus Brownia rhizoecola were also obtained, however, in the absence of ISH studies, the association between those organisms and the IMCs were not confirmed. The sequences identified in this study will allow for further molecular characterization of the microbial community associated with IMC infection in marine mollusks and their correlation with severity of the lesions to clarify their role as endosymbionts, commensals or true pathogens.

Environmental Sciences Europe, 2020

The Partnership for Chemicals Risk Assessment (PARC) is currently under development as a joint re... more The Partnership for Chemicals Risk Assessment (PARC) is currently under development as a joint research and innovation programme to strengthen the scientific basis for chemical risk assessment in the EU. The plan is to bring chemical risk assessors and managers together with scientists to accelerate method development and the production of necessary data and knowledge, and to facilitate the transition to next-generation evidence-based risk assessment, a non-toxic environment and the European Green Deal. The NORMAN Network is an independent, well-established and competent network of more than 80 organisations in the field of emerging substances and has enormous potential to contribute to the implementation of the PARC partnership. NORMAN stands ready to provide expert advice to PARC, drawing on its long experience in the development, harmonisation and testing of advanced tools in relation to chemicals of emerging concern and in support of a European Early Warning System to unravel th...

Environmental Sciences Europe, 2019

This article introduces the EU Horizon 2020 research project GRACE (Integrated oil spill response... more This article introduces the EU Horizon 2020 research project GRACE (Integrated oil spill response actions and environmental effects), which focuses on a holistic approach towards investigating and understanding the hazardous impact of oil spills and the environmental impacts and benefits of a suite of marine oil spill response technologies in the cold climate and ice-infested areas of the North Atlantic and the Baltic Sea. The response methods considered include mechanical collection in water and below ice, in situ burning, use of chemical dispersants, natural biodegradation, and combinations of these. The impacts of naturally and chemically dispersed oil, residues resulting from in situ burning, and non-collected oil on fish, invertebrates (e.g. mussels, crustaceans) and macro-algae are assessed by using highly sensitive biomarker methods, and specific methods for the rapid detection of the effects of oil pollution on biota are developed. By observing, monitoring and predicting oil movements in the sea through the use of novel online sensors on vessels, fixed platforms including gliders and the so-called SmartBuoys together with real-time data transfer into operational systems that help to improve the information on the location of the oil spill, situational awareness of oil spill response can be improved. Methods and findings of the project are integrated into a strategic net environmental benefit analysis tool (environment and oil spill response, EOS) for oil spill response strategy decision making in cold climates and ice-infested areas.

Science of The Total Environment, 2019

presents regional and seasonal changes that condition responses in mussels. • Food supply dictate... more presents regional and seasonal changes that condition responses in mussels. • Food supply dictated reproductive cycle and consequently biomarker response. • Seasonal variability unequally reflected at different biological organization levels.

Environmental science and pollution research international, Jan 24, 2017

This paper aims to contribute to the use of mangrove cupped oyster, Crassostrea rhizophorae, as a... more This paper aims to contribute to the use of mangrove cupped oyster, Crassostrea rhizophorae, as a biomonitor species for chemical contamination assessment in mangrove-lined Caribbean coastal systems. Sampling was carried out in eight localities (three in Nicaragua and five in Colombia) with different types and levels of contamination. Oysters were collected during the rainy and dry seasons of 2012-2013 and the tissue concentrations of metals, polycyclic aromatic hydrocarbons (PAHs), and persistent organic pollutants (POPs) were determined. Low tissue concentrations of metals (except Hg) and PAHs; moderate-to-high tissue concentrations of Hg, hexachlorocyclohexanes (HCHs), and dichlorodiphenyl-trichloroethanes (DDTs); detectable levels of chlorpyrifos, polychlorinated biphenyls (PCBs) (mainly CB28, CB118, CB138 and CB 153) and brominated diphenyl ethers 85 (BDE85); and negligible levels of musks were recorded in Nicaraguan oysters. A distinct profile of POPs was identified in Colombi...

Proceedings of MOL2NET, International Conference on Multidisciplinary Sciences, 2015

The growing concern due to the presence of plastics, especially micro and nanoplastics, in enviro... more The growing concern due to the presence of plastics, especially micro and nanoplastics, in environmental aquatic media requires the development of new methodologies to study the distribution of these particles and the effects that might cause in many organisms. In this work we have performed experiments using synthetic polystyrene microplastics (6-90 µm diameter) and mussels (Mytilus galloprovincialis) and we have studied the distribution of these particles by different techniques including FTIR and Raman spectroscopy, light and polarized light microscopy after being exposed for different periods of time (1-72 h). As a result of this work we were able to fine tune the preparation of the samples, from conservation to image and spectra analysis, and it was concluded that it was better to freeze the samples and to prepare the cryosections instead of embedding in paraffin. Regarding the light microscopy darkfield illumination offered less background signals than polarized one and therefore it was more suitable for small size particles. Finally, Raman spectroscopy allowed the characterization of the polystyrene particles better than FTIR allowing the development of image analysis techniques.

Estuarine, Coastal and Shelf Science, 2016

Oysters (Crassostrea gigas) farmed in a clean reference estuary were exposed to different combina... more Oysters (Crassostrea gigas) farmed in a clean reference estuary were exposed to different combinations of 63Cu and 107Ag stable isotope spikes in seawater at environmentally relevant concentrations during 28 days in order to study short-term bioaccumulation kinetics. Oysters were sampled, dissected and analyzed for Cu and Ag concentrations and isotope ratios (63/65Cu; 107/109Ag) after 7, 14, 21 and 28 days of exposure. Isotope ratios in oyster tissues showed significant differences from natural isotope ratios even after only 7 days of exposure, with relatively similar signals independent from the tissue. The changes in isotope ratios combined with conventional concentration measurements allowed precise quantification of 63Cu and 107Ag concentrations before experimental exposure, in the same individual oyster tissue. The high sensitivity and the possibility to precisely follow short-term accumulation at environmental levels of several (non radioactive) different metals in individual organisms are major advantages. Relatively constant Cu/Ag ratios in oysters exposed to the lowest Cu and Ag concentrations were observed. However, interactions between Cu and Ag accumulation occurred with (i) a synergy effect increasing Ag accumulation in the presence of high Cu concentrations and (ii) antagonism resulting in lower Cu accumulation at a given Cu exposure when Ag exposure levels increase. Comparison of experimentally accumulated Cu/Ag ratios (63Cuacc/107Agacc) with Cu/Ag in wildlife oysters along the French Atlantic coast (data provided by the national mussel watch program RNO/ROCCH) suggests that such metal ratios reflect, to a certain extent, exposure levels and proportions in the environment. As such, element ratios may indicate contamination sources better than metal concentrations alone. Metal accumulation in the different oyster tissues (mantle, muscle, gills, digestive gland and gonads) was rather homogeneous during the short-term direct exposure experiment, whereas wild oysters from the Gironde Estuary show very strong differences in metal accumulation between tissues, i.e. maximum Ag accumulation in digestive gland. This suggests that the trophic pathway (metals contaminating oysters through the food intake) may play a major role in wildlife oyster contamination.

Bulletin of Environmental Contamination and Toxicology, 2015

The present study was focused upon the assessment of acetyl-cholinesterase (AChE) activity in the... more The present study was focused upon the assessment of acetyl-cholinesterase (AChE) activity in the digestive gland (main metabolic center) and foot (highly innervated organ) of the green garden snail, Cantareus apertus (Born, 1778), exposed to different nominal dietary concentrations of Pb (25 and 2500 mg Pb/Kg), Cd (10 and 100 mg Cd/Kg) and their combination (25 mg Pb + 10 mg Cd/Kg and 2500 mg Pb + 100 mg Cd/ Kg) for 60 days. AChE activity was lower in the foot than in the digestive gland (~50%) and decreased with experimental time in both tissues. In metal treated snails, AChE activity was significantly decreased in both tissues to a 50-60% of the values recorded in control snails. This decrease followed a dose depending trend at each exposure time, albeit the response was attenuated at the long-term (60 d) in comparison with the short-term (7 d). Besides, the combination of both metals provoked interactive effects not seemingly related with the tissue levels of the metals. Thus, it was concluded that model toxic metals such as Pd and Cd cause a reduction in AChE activity in both studied tissues, more markedly at the short-term, although antagonistic effects were elicited by both metals in combination. As a whole, lowered AChE activity in C. apertus can be considered as a useful biomarker of the effects provoked by metals on cell signaling and therefore it may be suitable for ecosystem health assessment in metal polluted soils using this species as sentinel organism.

Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 1997

Recent studies show that the peroxisomal marker enzyme catalase is induced in the digestive epith... more Recent studies show that the peroxisomal marker enzyme catalase is induced in the digestive epithelium of mussels exposed to petroleum-derived hydrocarbons. The aim of the present study was to investigate whether this effect is accompanied by a proliferative response of the peroxisomes. Mussels were treated for 21, 49 and 91 days with three different doses of the water accommodated fraction

Elsevier Oceanography Series, 2004

... molecular, cellular and tissue-level biomarkers" an integrative approach Ionan Marig6mez... more ... molecular, cellular and tissue-level biomarkers" an integrative approach Ionan Marig6meza, Manu Soto~, Amaia Orbeaa, Ibon Cancio~and Miren P. Cajaravillea ... by those pollutants (Coles et al., 1995; Fagotti et al., 1996; Cajaraville et al., 1996; Pipe et al., 1999; Olabarrieta et al ...

Tissue and Cell, 1996

The localization of metals in selected tissues of metal-exposed mussels was investigated by means... more The localization of metals in selected tissues of metal-exposed mussels was investigated by means of autometallography. Mussels collected from a Zn-polluted site were (a) depurated or, alternatively, (b) exposed to either Cu, Zn or Cd for 41 d. Mussels collected from a clean site were used as experimental reference. Autometallographically demonstrated black silver deposits (BSD), indicating the presence of metals, were observed in gills, (a) in frontal cells and haemocytes of Cu-exposed mussels, (b) in secretory postlateral and abfrontal cells and in endothelial cells of Zn-exposed mussels, and (c) in frontal, postlateral and endothelial cells but mainly in abfrontal cells and haemocytes of Cd-exposed mussels. Autometallography also revealed the presence of BSD in connective tissue brown cells underlying the mantle. Additionally, adipogranular cells of the connective tissue surrounding the gonad follicles were positively stained but no BSD was found in gonad tissue. Scarce BSD were found in the cytoplasmic granules of the stomach wall of control mussels whilst depurating and metal-exposed mussels exhibited BSD lining the apex of the stomach epithelial cells. Contrary to the results previously obtained with marine gastropod molluscs, BSD were not found in the basal lamina of digestive tubules, stomach, and ducts of mussels. Highly conspicuous BSD were observed in digestive cell lysosomes of depurating and metal-exposed mussels. Basophilic cells were always devoid of BSD. Finally, BSD were also found in nephrocyte lysosomes and mineralized concretions.

Método para la identificación del sexo en peces. La presente invención se relaciona con el uso de... more Método para la identificación del sexo en peces. La presente invención se relaciona con el uso de una molécula asociada al ARN ribosómico 5S (ARNr 5S), o de una molécula implicada en el transporte de proteínas que intervienen en la regulación de la transcripción del gen que codifica el ARNr 5S, como marcador para identificar el sexo en peces. Asimismo, la invención también se relaciona con un método para clasificar por sexos una población de peces que comprende la determinación del nivel de expresión de una molécula asociada al ARNr 5S, o de una molécula implicada en el transporte de proteínas que intervienen en la regulación de la transcripción del gen que codifica el ARNr 5S.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the concentration on different amino acids and short chain organic acids in the whole body tissue of Mya arenaria.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the trypsin- and chymotrypsin-like proteasomal and lysosomal activity in gill tissue and digestive gland of Mya arenaria.

Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences freque... more Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the activity leucine aminopeptidase in gill tissue and digestive gland of Mya arenaria.